CN112034097A - Method for determining trapping and killing effect of prodenia litura adults - Google Patents
Method for determining trapping and killing effect of prodenia litura adults Download PDFInfo
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Abstract
The invention discloses a method for determining the trapping and killing effect of spodoptera litura adults, which comprises the following steps of (1) designing a test cell: preparing a plurality of closed test cells without prodenia litura, and equally dividing the test cells into a trapping and killing treatment region and a blank control region; (2) planting host plants: uniformly planting prodenia litura host plants in each test cell, wherein the prodenia litura host plants have the same growth vigor; (3) trapping and killing treatment; (4) inoculating insect pupa; (5) recording data; (6) and (5) calculating the trapping and killing effect. The method eliminates uncertain factors of outdoor evaluation, designs a larger closed cell, can meet the physiological life, mating and spawning activities of the prodenia litura, and can reflect the actual production situation, and other situations except that the prodenia litura cannot fly out and fly in are basically consistent with the natural state, so that the test data is ensured to be close to the natural state, the field test area is reduced, and the field test period is shortened.
Description
Technical Field
The invention belongs to the field of attractant evaluation, and particularly relates to a method for determining the trapping and killing effect of prodenia litura adults.
Background
Spodoptera litura Fabricius belongs to Lepidoptera, Spodoptera frugiperda, also called Neurospora litura, is a leaf-eating pest with strong overeating and omnivorous properties, and the harmed plants comprise dicotyledonous and monocotyledonous plants, and the total number is 389 in 109 families. The prodenia litura can eat leaves, buds, flowers and fruits of plants, and the larvae which are hatched initially eat the lower epidermis and mesophyll of the leaves, so that the epidermis of the leaves is transparent spots after the larvae are damaged; after four years of age, the patient enters the overeating period, and only the main veins are left after the leaves are eaten. Prodenia litura is a main pest on soybeans in south China.
At present, the main method for preventing and controlling prodenia litura still adopts chemical pesticide for prevention and control, but the large amount of chemical pesticide used for many years brings the results of reduction of the number of natural enemies, enhancement of the drug resistance of pests, environmental pollution, overproof agricultural product residue and the like.
The sex pheromone is a kind of measuring and forecasting means and new technology for controlling pests in the 60's of 20 th century, and it has the advantages of simplicity, high efficiency, no toxicity, no harm to beneficial pests, no environmental pollution, no resistance, etc. it is widely used in prediction, forecast, trapping and control of pests. The trapping and killing device for the prodenia litura is provided with the frequency vibration type insecticidal lamp at present, can effectively reduce the egg falling amount of pests, and has little damage to natural enemies, so the trapping and killing device is widely used for trapping and controlling the prodenia litura. However, the control effect of the sex attractant and the insecticidal lamp is not easy to determine, and most of the current determination methods are carried out in the field. The reason is that:
1. the adult flying capability is strong, and the control effect can not be reflected without large-area continuous control; however, when the control area is large enough, other control measures and blank control fields without control measures are not easy to find, even if found, the varieties and the growth of crops, the water and soil conditions and the insect source base number are different, and the determination of the control effect is difficult. The method is a common thing in production practice and also a difficulty in popularizing the pest adult trapping and killing technology.
2. The common sex-luring technology is generally used for luring and killing male insects, whether the male insects to be lured and killed are mated with female insects before being lured and killed is not well determined, if mating is finished, the male insects to be lured and killed cannot achieve the control effect, and the number of the male insects to be lured and killed is counted to serve as the control effect.
3. The light trapping and killing technology is not easy to determine whether mating or spawning is finished before the adult insect trapping and killing lamp is trapped and killed; if the male insect finishes mating or the female insect finishes laying eggs, whether the male insect is trapped and killed has no influence on the control effect, and generally, the number of trapped and killed adults can only be counted.
4. The harm situation of target pests in field is often investigated in production as the control effect, and the problem is that the adult pests can fly, and whether the adult pests are caused by the target pests flying from other areas or the target pests in the control area cannot be determined.
Patents and journals presenting the above problems we have found: 1. screening and drug effect comparison of prodenia litura biological insecticide, disclosed in Julipa and the like, volume 41, stage 8 in 2013 of Jiangsu agricultural science, page 127-128: and (3) performing a field efficacy test in a test field of a gardening station of Jinling science and technology institute in 2012 and 9-11 months, and designing a random block. Each variety is repeated for 3 times, and the total number of the varieties is 21 cells, and each cell is 5-10 m2And planting 10-15 eggplants, and performing conventional cultivation management. The treatment of the test medicament and blank control in the plot adopts random block arrangement, more than 80% of prodenia litura in the field is in 2-3 instars, and the plant is uniformly sprayed. The amount of the liquid medicine is 900kg/hm2. Respectively investigating all the insect quantities in the cell 1, 3 and 5 days after treatment, and calculating the prevention and treatment effect; 2. application No.: 201710161644.2, title of the invention: prodenia litura sex pheromone composition and lure, specification [0054]The method disclosed in the paragraph: sixthly, field investigation and field test are carried out on the experimental site set point of agricultural science research institute in Hanzhong city of Shaanxi province from 7 months to 8 months in 2015. The traps of different treatments are randomly arranged in a chessboard shape, 3 bamboo poles are taken to be made into a tripod, the tripod is placed in a vegetable field, a basin is placed on the tripod, the basin is about 80cm away from the ground, the diameter of the basin is 24cm, the basin is filled with water, and about 1g of washing powder is placed in the basin. Then the lure core is fixed above the basin by threading the iron wire, so that the lure core is about 1cm away from the water surface. The setting density of the lure cores is 5 per mu, the lure cores are arranged in a straight line, the distance between the lure cores is 20m, and the protection rows (6.5m) are arranged on the periphery. The attracted insects are counted and cleaned every morning, and meanwhile, the water in the pot is supplemented and the height of the lure is adjusted. The lure was changed every three days to reduce the influence of the position.
In order to solve the technical problem of influence of outdoor factors, the application number is as follows: 201710286317.X, title of invention: a method for biologically preventing and controlling the harm of dendrobium officinale prodenia litura, which is applied in the specification [0052 ]]Paragraph proposes a method of determination: in this example, the control effect of different test treatments was measured outdoors by the following specific method: inoculating Spodoptera litura test insects to healthy plants in a healthy dendrobium officinale planting greenhouse every 5m2As a test cell, 20 test insects are placed in each cell, and the test insects are repeated for 3 times, and each cell is separated by an insect-proof net. After the test insects normally feed, the stock solutions obtained in example 1 and example 2 and 50% phoxim emulsion are diluted 1000 times and applied to the cells with the test insects, and the mortality rate is counted. However, this method does not distinguish between males and females of the test insects, and has the problems described above in the 2 nd and 3 rd points.
Disclosure of Invention
The invention aims to solve the technical problems and provides a method for measuring the trapping and killing effect of spodoptera litura imagoes, which is close to a natural state, has more accurate measuring result, shortens the field test period and reduces the test area.
In order to achieve the purpose, the technical scheme of the invention is as follows:
a method for determining the trapping and killing effect of spodoptera litura comprises the following steps:
(1) designing a test cell: preparing a plurality of closed test cells without prodenia litura (if prodenia litura occurs, cleaning is needed in advance, so that prodenia litura does not exist in the test cells), and equally dividing the test cells into a trapping and killing treatment region and a blank control region;
(2) planting host plants: uniformly planting prodenia litura host plants in each test cell, wherein the prodenia litura host plants have the same growth vigor;
(3) trapping and killing treatment: installing a trapping device to be measured in the trapping treatment area;
(4) and (3) worm pupa inoculation: classifying pupas of prodenia litura according to male and female, placing the prodenia litura pupas in test cells 2 days before eclosion, placing the prodenia litura pupas in each test cell according to the ratio of male to female of 1:1, and placing food which needs to be eaten by eclosion adults near the pupas;
(5) and (3) data recording: adult food is supplemented every day in time, the number a of female adults after eclosion in each test cell (namely, the number of pupa shells left after female pupa eclosion) is counted, and the whole test is finished after all the adults die and eggs are hatched. Putting the egg mass of each test cell into a numbered container, after hatching, respectively recording the number b of the larvae of each test cell, and counting the number A of the female adults in the trapping and killing treatment areaP(i.e. the sum of the numbers a of the female adults in each trapping and killing area) and hatchingLarva number Bp(i.e. sum of larva number b of each trapping and killing treatment area), and female adult number A in blank control area0(i.e., the sum of the numbers of female adults a in the placebo zone) and the number of hatched larvae B0(i.e. the sum of the larva number b of the blank control area), and then calculating the larva number C of each female adult egg-laying hatching in each trapping and killing treatment areap＝Bp/APThe average number of larvae C hatched by each female adult egg laying station in the blank control area0＝B0/A0；
(6) Calculating the trapping and killing effect: taking the steps (1) - (5) as a test, repeating the test for n times under the same condition, wherein n is more than or equal to 3, calculating the average value of the number of larvae incubated by each female adult egg-laying station, namely the average value of the number of larvae, and the average value of the number of larvae in each trapping and killing treatment area
Average number of larvae in blank control zone
Further calculating the trapping and killing effect D of the trapping and killing agent of each trapping and killing device to be measured,
as a further technical scheme, the trapping and killing device is a light trapping and killing device or a sex trapping and killing device.
As a further technical scheme, the test community is a closed net room capable of preventing prodenia litura from flying out and flying in, and one end of each net room is provided with an inlet room convenient for testers to enter and exit the net room.
As a further technical scheme, when the trapping and killing device is a lamplight trapping and killing device, the net chamber of the trapping and killing treatment area is a long net chamber, the length of the long net chamber is 90-100 meters, the width of the long net chamber is 3-4 meters, the net chamber of the blank control area is a short net chamber, the length of the short net chamber is 10-15 meters, and the width of the short net chamber is 3-4 meters; when the trapping device is a sexual trapping device, the net chambers of the trapping treatment area and the blank control area are both short net chambers, and the short net chambers are 10-15 meters long and 3-4 meters wide.
As a further technical scheme, the light trapping and killing device is an insect killing lamp capable of trapping and killing prodenia litura; the sex attractant trapping and killing device is a trapping core (containing sex attractant for prodenia litura) for the sex attraction of prodenia litura and a corresponding trapping bottle thereof. These are all products commonly used in the market, and therefore, the structure or the components thereof will not be described in detail.
As a further technical scheme, the prodenia litura host plant is a taro seedling or a corn seedling.
As a further technical scheme, the number of clusters of taro seedlings or corn seedlings in each net room is calculated according to the following formula:
Y＝(L/5)+1；
wherein, Y: the cluster number and cluster of taro seedlings or corn seedlings in each net room;
l: length of each wire chamber, meter;
each group of taro seedlings or corn seedlings is 10-30, and each group of taro seedlings or corn seedlings is uniformly distributed in a Z shape in the length direction in the net room.
As a further technical scheme, the noctuid twill pupae are inoculated into a room according to the proportion that two moth pupae are inoculated on each cluster of taro seedlings or corn seedlings; the two-pier pupas comprise 1-pier male pupa and 1-pier female pupa (the male-female ratio of the prodenia litura raised in nature and laboratories is simulated and is generally 1:1) which are equal in quantity, and the quantity of the pupas in each pier is 10-20; the number of the twill noctuids pupae and the number of the taro seedlings or the corn seedlings in the unit area of each test cell are equal, so that the consistency of each treatment test condition is ensured.
As a further technical scheme, the food eaten by the adults is honey water, and the volume content of honey in the honey water is 10-15%.
As a further technical scheme, the entrance chamber comprises an inner door, a containing room and an outer door, the containing room is a completely closed room, and the containing room is connected to one end of the net room; the inner door is arranged at the joint of the net room and the accommodating room and is communicated with the net room and the accommodating room; the outer door is arranged on the other side of the accommodating room and is communicated with the outside; the trapping and killing device is arranged at one end of the net room in the length direction, and the inlet room is arranged at the other end of the net room in the length direction.
Compared with the prior art, the invention has the beneficial effects that:
1. the method eliminates uncertain factors of outdoor evaluation, designs a larger closed cell, can meet the physiological life, mating and spawning activities of the prodenia litura, and also conforms to the actual production situation, except that the prodenia litura cannot fly out and fly in, other situations are basically consistent with the natural state, so that the test data is ensured to be close to the natural state, the field test area is reduced, and the field test period is shortened.
2. Because the sex luring and killing device is specially used for luring and killing male prodenia litura adults, the insecticidal lamp can lure and kill male and female prodenia litura adults, the prodenia litura is placed according to the classification of male and female (the male-female ratio of the prodenia litura raised in the nature and laboratories is simulated and is generally 1:1), and because the average densities of host plants and prodenia litura in each cell are equal, the luring and killing effect of the final luring and killing device is determined by calculating the number of larvae hatched by each female adult egg. (1) The statistics of trapping and killing the device to prodenia litura control effect has been simplified, has reflected the production practice, and the prodenia litura is that the harm that causes the crop is got to eat through the larva and is caused, and other insect modes of the prodenia litura such as adult, pupa and ovum do not do harm to the crop, and the larva quantity how much has decided the prodenia litura is to the harm degree of crop, and the purpose that people controlled prodenia litura just reduces and causes harm to the crop. (2) The net room completely isolates the prodenia litura inside and outside the test area, so that the test error caused by the fact that the prodenia litura imagoes in the test area fly out of the test area (larvae of the prodigy and hatching of the prodenia litura do not harm the crops in the test area) and the prodenia litura imagoes outside the test area fly into the test area (larvae of the prodigy and hatching of the prodigy and the crops in the. (3) In the production practice, male adults complete mating or female adults complete spawning and are trapped and killed, so that if the trapping and killing effect is evaluated by counting the adults, although the adults are killed, the offspring larvae still damage crops, and the trapping and killing effect of the trapping and killing device is higher in evaluation, the trapping and killing effect D of the final trapping and killing device is determined by calculating the number of larvae hatched by each female adult spawning, the higher the D is, the smaller the average number of larvae in a trapping and killing treatment area is, and the better the trapping and killing effect of the to-be-determined trapping and killing device on prodenia litura adults is; the lower the D is, the more the average value of the number of the larvae in the trapping and killing processing area is, the poorer the trapping and killing effect of the trapping and killing device to be determined on the prodenia litura imagoes is, the statistical avoidance of the statistics of the number of the trapping and killing imagoes as the index of the control effect is realized, and the error is reduced.
3. The determination method can be used for the prodenia litura and can also be used for determining the trapping and killing effects of other trapping and killing devices on other similar pests and imagoes, such as the pests of spodoptera frugiperda, tryporyza incertulas, cotton bollworms, spodoptera exigua and the like.
4. In the production practice, the pest control area of one pest killing lamp is 30-50 mu. In the test, it is very difficult to find a repetitive region where other conditions including crop species, crop growth, soil moisture, cultivation management, and the like are consistent. According to the invention, 3 net rooms with the length of 95 meters are prepared for the trapping and killing test of the insecticidal lamps, the circular area with the radius of 95 meters is about 42.52 mu according to a circular area calculation formula, the insecticidal lamps are placed at one end of the net room, the control effect of the insecticidal lamps on pests at the other end can be investigated, the problem of the area of one insecticidal lamp is solved, and the requirements of other factors on basic consistency are also met. In the production practice, the control area of the spodoptera litura sex trapping and killing device on the spodoptera litura is generally 1 mu, and the requirement can be met by a short net room with the length of 15 meters.
Drawings
FIG. 1 is a schematic structural diagram of a long net chamber in a trapping and killing treatment area in the method for determining the trapping and killing effect of the imago of prodenia litura of the present invention;
FIG. 2 is a schematic structural diagram of a short net chamber in a trapping and killing treatment area in the method for determining the trapping and killing effect of the spodoptera litura;
FIG. 3 is a schematic structural diagram of a short net room in a blank control area in the method for determining the adult prodenia litura trapping and killing effect of the present invention;
reference numerals: 1-long net room, 2-host plant, 3-entrance room, 31-inner door, 32-holding room, 33-outer door, 4-light trapping device, 5-short net room, 6-sex trapping device.
Detailed Description
The present invention will be described in further detail with reference to examples, but the embodiments of the present invention are not limited to the scope of the examples.
As shown in fig. 1-3, a method for determining the trapping and killing effect of spodoptera litura comprises the following steps:
(1) designing a test cell: preparing a plurality of closed test cells without prodenia litura (if prodenia litura occurs, cleaning is needed in advance, so that prodenia litura does not exist in the test cells), and equally dividing the test cells into a trapping and killing treatment region and a blank control region;
(2) planting host plants: uniformly planting prodenia litura host plants in each test cell, wherein the prodenia litura host plants have the same growth vigor;
(3) trapping and killing treatment: installing a trapping device to be measured in the trapping treatment area;
(4) and (3) worm pupa inoculation: classifying pupas of prodenia litura according to male and female, placing the prodenia litura pupas in test cells 2 days before eclosion, placing the prodenia litura pupas in each test cell according to the ratio of male to female of 1:1, and placing food which needs to be eaten by eclosion adults near the pupas;
(5) and (3) data recording: adult food is supplemented every day in time, the number a of female adults after eclosion in each test cell (namely, the number of pupa shells left after female pupa eclosion) is counted, and the whole test is finished after all the adults die and eggs are hatched. Putting the egg mass of each test cell into a numbered container, after hatching, respectively recording the number b of the larvae of each test cell, and counting the number A of the female adults in the trapping and killing treatment areaP(i.e. the sum of the number a of female adults in each trapping and killing area) and the number B of hatched larvaep(i.e., sum of larvae number b of each trap-treated area), blank control areaNumber of female adults A0(i.e., the sum of the numbers of female adults a in the placebo zone) and the number of hatched larvae B0(i.e. the sum of the larva number b of the blank control area), and then calculating the larva number C hatched by each female adult egg laying of the trapping and killing treatment areap＝Bp/APThe number of larvae C hatched by each female adult egg-laying station in the blank control area0＝B0/A0；
(6) Calculating the trapping and killing effect: taking the steps (1) - (5) as a test, repeating the test for n times under the same condition, wherein n is more than or equal to 3, and calculating the average value of the number of larvae hatched by each female adult egg-laying station, namely the average value of the number of larvae, and the average value of the number of larvae in a trapping and killing treatment area
Average number of larvae in blank control zone
C0n) N, further calculating the trapping and killing effect D of each trapping and killing device to be measured,
the trapping device is a light trapping device or a sex trapping device. The test district is the closed net room that can prevent the prodenia litura departure and fly into, and the one end of every net room is equipped with the entry room 3 that is convenient for the tester to pass in and out the net room. When the trapping and killing device is a lamplight trapping and killing device 4, the net chamber of the trapping and killing processing area is a long net chamber 1, the long net chamber 1 is 90-100 meters long and 3-4 meters wide, the net chamber of the blank control area is a short net chamber 5, the short net chamber 5 is 10-15 meters long and 3-4 meters wide; when the trapping device is a sex trapping device 6, the net chambers of the trapping treatment area and the blank control area are both short net chambers 5, the length of each short net chamber is 10-15 meters, and the width of each short net chamber is 3-4 meters.
The light trapping and killing device 4 is an insect killing lamp capable of trapping and killing prodenia litura; the sex attractant killing device 6 is an attraction core (containing sex attractant for prodenia litura) aiming at sex attraction of prodenia litura and a corresponding attraction bottle. These are all products commonly used in the market, and therefore, the structure or the components thereof will not be described in detail. The prodenia litura host plant 2 is taro seedling or corn seedling. The number of clusters of taro seedlings or corn seedlings in each net chamber is calculated according to the following formula:
Y＝(L/5)+1；
wherein, Y: the cluster number and cluster of taro seedlings or corn seedlings in each net room;
l: length of each wire chamber, meter;
each group of taro seedlings or corn seedlings is 10-30, and each group of taro seedlings or corn seedlings is uniformly distributed in a Z shape in the length direction in the net room.
Inoculating two pupas of Tsuga twill into the room according to the proportion of each cluster of taro seedlings or corn seedlings inoculated with the two pupas of Tsuga twill; the two-pier pupa comprises 1 pier male pupa and 1 pier female pupa (the male-female ratio of the prodenia litura raised in nature and laboratories is simulated and is generally 1:1) with equal amount, and the number of pupas in each pier is 10-20; the number of the twill noctuids pupae and the number of the taro seedlings or the corn seedlings in the unit area of each test cell are equal, so that the consistency of each treatment test condition is ensured.
The food for adult is Mel solution, and the Mel content in the Mel solution is 10-15% by volume.
The entrance room comprises an inner door 31, a containing room 32 and an outer door 33, the containing room 32 is a completely closed room, and the containing room 32 is connected with one end of the net room; the inner door 31 is arranged at the joint of the net room and the accommodating room 32 and is communicated with the net room and the accommodating room 32; an outer door 33 installed at the other side of the receiving room 32 to communicate the receiving room 32 with the outside; the trapping and killing device is arranged at one end of the net room in the length direction, and the inlet room 3 is arranged at the other end of the net room in the length direction.
Example 1:
1. web room design and processing arrangement.
The width of the net room is uniformly set to be 3 meters.
3 long net rooms 1 are prepared for the trapping and killing test of the insecticidal lamp, the length is 95 meters, and according to a circular area calculation formula, the circular area with the radius of 95 meters is about 2.84 hectares (about 42.53 mu), which is equivalent to the prevention and control area of 30-50 mu of an insecticidal lamp in production practice. (95 m × 3 m 285 m, 285 x 3 m 855 m)
6 short wire chambers 5 are prepared, 15 meters in length, more than one sixth of 95 meters. The same area of the net chamber is put into the same number of test insects, 3 of which are used for sex attraction test and 3 of which are treated by blank control. (15 m.times.3 m.45 m.square, 45 x.6 m.270 m.square)
Total area of mesh chamber: 855+270 ═ 1125 square (about 1.69 mu)
Three treatments were designed: insecticidal lamp trap (purchased from cortexas multidisciplinary trade, ltd.), sex attractant trap (purchased from ningbon biotechnology ltd) and blank control, each treatment was 3 replicates for 9 cells.
2. Host plant (corn seedling)
Starting from the opposite direction of the trapping and killing device, corn seedlings are placed (the distance is calculated by the central point of the cluster), 4 clusters can be placed in a short net chamber with the length of 15 meters, 20 clusters can be placed in a short net chamber with the length of 95 meters, 4 multiplied by 6 is 24 (cluster), 20 multiplied by 3 is 60 (cluster), and 84 clusters are counted in a small scale. The square is placed, the groups are uniformly distributed in a Z shape, and sidewalks are reserved for the square to facilitate investigation. 25 maize seedlings per colony.
3. Pupa of test insect prodenia litura
Is placed above the corn seedlings and is far away from the trapping and killing device, namely, is placed on the opposite side of the square corn seedlings relative to the trapping and killing device. One cluster of taros and two piers of pupae (15 per pier with separated male and female), the specific quantity is calculated by the net room area, and the pupae quantity per unit area is equal.
4. Procedure of the test
The end (94 meters, relative to 41.64 mu of control) of 3 long net rooms 1 (namely the lamp trapping treatment area) is provided with one insecticidal lamp to be detected for trapping and killing effects, and the lamp is turned on every day in the dark and turned off every day; 3 short net chambers (namely sex-attracting treatment areas) are provided with spodoptera litura sex-attracting bottles and corresponding attracting cores to be detected for trapping and killing effects at 14.5 meters (equivalent to controlling 1.0 mu). 3 short net chambers (i.e., blank control treatment zones) did not contain a trap.
Classifying the pupae of the prodenia litura according to male and female, uniformly placing the prodenia litura pupae into a test net room according to a proportion that the male and female in a square area are respectively placed in a test net room 2 days before eclosion, placing 10% honey water near the pupae for adult insects to eat, and waiting for eclosion.
And observing and recording the eclosion of pupae, trapping imagoes, oviposition of the imagoes and the like at 8 am every day, and finishing the whole test after all the imagoes die and the eggs are hatched.
5. Test record and its concrete process
Surveying at 8 am every day, starting from the opposite side of the trapping and killing device, firstly checking and recording the eclosion condition of pupae, and taking out pupae shells, wherein 2 persons form a group; checking whether the nutrient-supplemented honey water is enough, and adding a little if the honey water is dry; and finally checking the egg laying condition, picking off egg blocks, counting the number of the egg blocks, recording, putting into a vessel, labeling, and putting into a box.
Calculating the average value of the number of larvae of each female adult laying eggs and hatching in the insecticidal lamp processing area through the original data record
p＝(Cp1+Cp2+…+Cpn) V, average number of larvae hatched per egg laying of female adults in sex attractant treatment area
C’p2+…+C’pn) V, average number of larvae hatched and oviposited per female adult in the placebo zone
Further calculating the trapping and killing effect D of the insecticidal lamp processing area,
the trapping and killing effect D' of the sexual trapping and killing treatment area,
the trapping and killing effect of the insecticidal lamp treatment area is as follows: 20.56 percent
The trapping and killing effect of the sexual trapping and killing treatment area is as follows: 13.34 percent.
The above-described embodiments are only specific examples for further explaining the object, technical solution and advantageous effects of the present invention in detail, and the present invention is not limited thereto. Any modification, equivalent replacement, improvement and the like made within the scope of the present disclosure are included in the protection scope of the present invention.
Claims (10)
1. A method for measuring the trapping and killing effect of spodoptera litura adults is characterized by comprising the following steps:
(1) designing a test cell: preparing a plurality of closed test cells without prodenia litura, and equally dividing the test cells into a trapping and killing treatment region and a blank control region;
(2) planting host plants: uniformly planting prodenia litura host plants in each test cell, wherein the prodenia litura host plants have the same growth vigor;
(3) trapping and killing treatment: installing a trapping device to be measured in the trapping treatment area;
(4) and (3) worm pupa inoculation: classifying pupas of prodenia litura according to male and female, placing the prodenia litura pupas in test cells 2 days before eclosion, placing the prodenia litura pupas in each test cell according to the ratio of male to female of 1:1, and placing food which needs to be eaten by eclosion adults near the pupas;
(5) and (3) data recording: supplementing adult food every day in time, counting the number a of the female adults after eclosion in each test cell, and finishing the whole test after all the adults die and eggs are hatched; in the test process, the egg mass of each test cell is placed in a numbered container, after incubation, the number b of the larva of each test cell is recorded, and the number A of the female adults in the trapping and killing treatment area is countedPAnd the number of hatched larvae BpAnd the number of female adults in the blank control area A0And the number of hatched larvae B0And then calculating the oviposition of each female adult in the trapping and killing treatment areaNumber of hatched larvae Cp＝Bp/APThe number of larvae C hatched by each female adult egg-laying station in the blank control area0＝B0/A0；
(6) Calculating the trapping and killing effect: taking the steps (1) to (5) as a test, repeating the test for n times under the same condition, wherein n is more than or equal to 3, calculating the average value of the number of larvae incubated by each female adult egg, namely the average value of the number of larvae, and the average value of the number of larvae in each trapping and killing treatment area
Average number of larvae in blank control zone
Further calculating the trapping and killing effect D of the trapping and killing agent of each trapping and killing device to be measured,
2. the method for determining the trapping and killing effect of the spodoptera litura imago as claimed in claim 1, wherein the method comprises the following steps: the trapping and killing device is a light trapping and killing device or a sex trapping and killing device.
3. The method for determining the trapping and killing effect of the spodoptera litura imago as claimed in claim 2, wherein the method comprises the following steps: the test community is the closed net room that can prevent the prodenia litura departure and fly into, and the one end of every net room is equipped with the entry room that is convenient for the tester business turn over net room.
4. The method for determining the trapping and killing effect of the prodenia litura adults according to claim 3, which is characterized in that: when the trapping and killing device is a lamplight trapping and killing device, the net chamber of the trapping and killing processing area is a long net chamber, the length of the long net chamber is 90-100 meters, the width of the long net chamber is 3-4 meters, the net chamber of the blank comparison area is a short net chamber, the length of the short net chamber is 10-15 meters, and the width of the short net chamber is 3-4 meters; when the trapping device is a sexual trapping device, the net chambers of the trapping treatment area and the blank control area are both short net chambers, and the short net chambers are 10-15 meters long and 3-4 meters wide.
5. The method for determining the trapping and killing effect of the prodenia litura adults according to claim 4, which is characterized in that: the lamplight trapping and killing device is an insecticidal lamp capable of trapping and killing prodenia litura; the sex attractant trapping and killing device is a trapping core aiming at sex attraction of prodenia litura and a corresponding trapping bottle thereof.
6. The method for determining the trapping and killing effect of the prodenia litura adults according to claim 3, which is characterized in that: the prodenia litura host plant is a taro seedling or a corn seedling.
7. The method for determining the trapping and killing effect of the prodenia litura imagoes, according to claim 6, is characterized in that: the number of clusters of taro seedlings or corn seedlings in each net chamber is calculated according to the following formula:
Y＝(L/5)+1；
wherein, Y: the cluster number and cluster of taro seedlings or corn seedlings in each net room;
l: length of each wire chamber, meter;
each group of taro seedlings or corn seedlings is 10-30, and each group of taro seedlings or corn seedlings is uniformly distributed in a Z shape in the length direction in the net room.
8. The method for determining the trapping and killing effect of the spodoptera litura imago as claimed in claim 7, wherein the method comprises the following steps: the noctuid twill pupas are inoculated into the room according to the proportion that two dollops of pupas are inoculated to each cluster of taro seedlings or corn seedlings; the two-pier pupas comprise 1-pier male pupa and 1-pier female pupa which are equal in quantity, and the number of the pupas in each pier is 10-20; the number of the twill noctuid pupae and the number of the taro seedlings or the corn seedlings in the unit area of each test cell are equal.
9. The method for determining the trapping and killing effect of the spodoptera litura imago as claimed in claim 1, wherein the method comprises the following steps: the food eaten by the adults is honey water, and the volume content of honey in the honey water is 10-15%.
10. The method for determining the trapping and killing effect of the prodenia litura imagoes according to any one of claims 3 to 9, wherein the method comprises the following steps: the entrance chamber comprises an inner door, a containing room and an outer door, the containing room is a completely closed room, and the containing room is connected to one end of the net room; the inner door is arranged at the joint of the net room and the accommodating room and is communicated with the net room and the accommodating room; the outer door is arranged on the other side of the accommodating room and is communicated with the outside; the trapping and killing device is arranged at one end of the net room in the length direction, and the inlet room is arranged at the other end of the net room in the length direction.
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Citations (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2004028248A1 (en) * | 2002-09-26 | 2004-04-08 | James Daniel Forehand | Method and apparatus for killing insects by trapping larvae |
| US20070056207A1 (en) * | 2005-09-14 | 2007-03-15 | Chi-Wen Chen | Electric insect trap |
| US20070193109A1 (en) * | 2004-02-28 | 2007-08-23 | Cesco Co., Ltd. Cesco B/D | Cockroach trap with improved capturing rate and remote monitoring system using the same |
| CN101113977A (en) * | 2007-03-27 | 2008-01-30 | 广州甘蔗糖业研究所 | Method for rapidly identifying resistance of transgenic Bt sugarcane to borer |
| CN101984799A (en) * | 2010-11-09 | 2011-03-16 | 浙江省农业科学院 | Method for continuously propagating large amount of Cnaphalocrocis medinalis in field |
| CN102175845A (en) * | 2011-02-22 | 2011-09-07 | 山东农业大学 | Method for monitoring drug resistance of spodostera exigua hubner to insecticides |
| CN103782965A (en) * | 2014-02-08 | 2014-05-14 | 云南农业大学 | Method for testing influence of corn volatile matter on oviposition behavior of Asiatic corn borer |
| CN104322461A (en) * | 2014-11-15 | 2015-02-04 | 云南省农业科学院甘蔗研究所 | Indoor artificial feeding method for sesamia inferen |
| CN104535748A (en) * | 2014-12-30 | 2015-04-22 | 中国农业科学院柑桔研究所 | Outdoor icerya purchasi simulation biological activity assay and pesticide effectiveness evaluation method and application thereof |
| CN105960979A (en) * | 2016-05-05 | 2016-09-28 | 浙江省农业科学院 | Method for improving sesamia inferen control effect by coordinative application of trap crop and sex attractant |
| CN106416715A (en) * | 2016-10-10 | 2017-02-22 | 南京农业大学 | Identification method for selection resistance of prodenia litura larva living bodies at soybean seedling stage |
| GB201813146D0 (en) * | 2018-08-13 | 2018-09-26 | Starfield Enhance S Pte Ltd | Mosquito eradicator |
| CN109121903A (en) * | 2018-06-21 | 2019-01-04 | 张家港市金阳生态农业科技有限公司 | A kind of method of efficient integrated control Kiwi berry prodenia litura |
-
2020
- 2020-07-23 CN CN202010727660.5A patent/CN112034097B/en active Active
Patent Citations (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2004028248A1 (en) * | 2002-09-26 | 2004-04-08 | James Daniel Forehand | Method and apparatus for killing insects by trapping larvae |
| US20070193109A1 (en) * | 2004-02-28 | 2007-08-23 | Cesco Co., Ltd. Cesco B/D | Cockroach trap with improved capturing rate and remote monitoring system using the same |
| US20070056207A1 (en) * | 2005-09-14 | 2007-03-15 | Chi-Wen Chen | Electric insect trap |
| CN101113977A (en) * | 2007-03-27 | 2008-01-30 | 广州甘蔗糖业研究所 | Method for rapidly identifying resistance of transgenic Bt sugarcane to borer |
| CN101984799A (en) * | 2010-11-09 | 2011-03-16 | 浙江省农业科学院 | Method for continuously propagating large amount of Cnaphalocrocis medinalis in field |
| CN102175845A (en) * | 2011-02-22 | 2011-09-07 | 山东农业大学 | Method for monitoring drug resistance of spodostera exigua hubner to insecticides |
| CN103782965A (en) * | 2014-02-08 | 2014-05-14 | 云南农业大学 | Method for testing influence of corn volatile matter on oviposition behavior of Asiatic corn borer |
| CN104322461A (en) * | 2014-11-15 | 2015-02-04 | 云南省农业科学院甘蔗研究所 | Indoor artificial feeding method for sesamia inferen |
| CN104535748A (en) * | 2014-12-30 | 2015-04-22 | 中国农业科学院柑桔研究所 | Outdoor icerya purchasi simulation biological activity assay and pesticide effectiveness evaluation method and application thereof |
| CN105960979A (en) * | 2016-05-05 | 2016-09-28 | 浙江省农业科学院 | Method for improving sesamia inferen control effect by coordinative application of trap crop and sex attractant |
| CN106416715A (en) * | 2016-10-10 | 2017-02-22 | 南京农业大学 | Identification method for selection resistance of prodenia litura larva living bodies at soybean seedling stage |
| CN109121903A (en) * | 2018-06-21 | 2019-01-04 | 张家港市金阳生态农业科技有限公司 | A kind of method of efficient integrated control Kiwi berry prodenia litura |
| GB201813146D0 (en) * | 2018-08-13 | 2018-09-26 | Starfield Enhance S Pte Ltd | Mosquito eradicator |
Non-Patent Citations (4)
| Title |
|---|
| ROBERT L.ET AL.: ""Trapping noctuid moths with synthetic floral volatile lures"", 《ENTOMOLOGIA EXPERIMENTALIS ET APPLICATA》, vol. 3, no. 103, 1 June 2002 (2002-06-01), pages 219 - 226 * |
| WILSON BARROS-PARADA ET AL.: ""An evaluation of orange and clear traps with pear ester to monitor codling moth (Lepidoptera: Tortricidae) in apple orchards"", 《CIECIA INVESTIGACION AGRARIA》, vol. 40, no. 2, 7 November 2013 (2013-11-07), pages 307 - 315 * |
| 王建军 等: ""单波长太阳能灯诱杀核桃楸大蚕蛾"", 《东北林业大学学报》, vol. 42, no. 11, 6 November 2014 (2014-11-06), pages 134 - 137 * |
| 王恩国 等: ""性诱剂对西兰花主要害虫综合控制效果及其田间诱杀技术研究"", 《农业学报》, vol. 2, no. 1, 20 January 2012 (2012-01-20), pages 18 - 25 * |
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