CN104535748A - Outdoor icerya purchasi simulation biological activity assay and pesticide effectiveness evaluation method and application thereof - Google Patents
Outdoor icerya purchasi simulation biological activity assay and pesticide effectiveness evaluation method and application thereof Download PDFInfo
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- CN104535748A CN104535748A CN201410842556.5A CN201410842556A CN104535748A CN 104535748 A CN104535748 A CN 104535748A CN 201410842556 A CN201410842556 A CN 201410842556A CN 104535748 A CN104535748 A CN 104535748A
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Abstract
The invention discloses an outdoor icerya purchasi simulation biological activity assay and pesticide effectiveness evaluation method. The method includes the steps that natural inoculation and feeding are conducted on tested insects; supplied test fungicides are prepared into liquid of a set concentration; branches with insects of potted nursery stock are integrally soaked into the prepared liquid, and then the soaked branches with insects are enveloped by a thin and soft gauze element; the potted nursery stock which is soaked with pesticide is placed in a phytotron with the light dark period being L:D=14:10 h, the temperature being 25+/-1 DEG C and the relative humidity being 75% to be fed; the death and survival condition of the tested icerya purchasi is examined after the icerya purchasi is fed for 24 hours, and the death number and the survival number are recorded; a living body plant is used for cultivating the tested insects which are soaked with the liquid, the food freshness of the icerya purchasi is guaranteed, sufficient nutrients are provided for the icerya purchasi, testing is conducted under a natural environment growth condition where the icerya purchasi is placed, the accuracy of biological assay results is effectively improved, hence, the icerya purchasi population resistance level can be known comprehensively, accurately and precisely, and a basis is provided for reasonably utilizing pesticide and making a resistance treatment plan accurately.
Description
Technical field
The present invention relates to insect pest of the plant prevention and control field, particularly outside a kind of simulating chamber, blow cotton a red-spotted lizard biological activity determination and evaluation of pesticide effectiveness method and application thereof.
Background technology
Blow cotton a red-spotted lizard and belong to Arthropoda, Insecta, Homoptera, Shuo Jie section, blow cotton a red-spotted lizard and belong to.Blow the plants such as cotton a red-spotted lizard harm Rutaceae, pulse family, composite family, the rose family and Solanaceae.Suck tree body juice, bring out dark mildew, cause fallen leaves, spike top, tree vigo(u)r weak.Blow cotton a red-spotted lizard 1st generation adult and occur in the 5-6 month, 2nd generation is in late July to early August, and the 3rd generation is in October.In Chongqing, within 1 year, there is 3-4 generation.Female adult pest with 4,7-8,10-11 be maximum.Female adult pest focuses mostly on greatly and fixes a place, the cotton-shaped wax of abdomen end secretion white cotton, lays eggs in secretion wax silk limit, limit.Blowing the cotton a red-spotted lizard egg-laying season reached more than January.In natural situation, male worm quantity is few, seeks single-female generation more.After casting off a skin for twice, change residence once.After 2nd age, forward to gradually on limb and live in concentrated communities harm, suck resin, drain honeydew simultaneously.Indoor bioassay be a kind of can more comprehensively, more accurately understand the means of insect field population resistance level, also be a kind of method of comparatively traditional understanding resistance, now widely used various bioassay method, seedling method, drop method is soaked as being with leaf, to large for examination insect damage, contrast mortality ratio is large, the raw accuracy surveying result of impact.
Summary of the invention
In view of this, the object of the present invention is to provide and blow cotton a red-spotted lizard biological activity determination and evaluation of pesticide effectiveness method outside a kind of simulating chamber, live plant is adopted to cultivate the examination worm soaking liquid, ensure to blow the fresh of cotton a red-spotted lizard food and the nutrient that abundance is provided for blowing cotton a red-spotted lizard, the outdoor elements growth conditions that cotton a red-spotted lizard is blown in simulation is tested, the accuracy of effective raising bioassay results and accuracy, thus cotton a red-spotted lizard population resistance level is blown in comparatively comprehensively accurate and accurate understanding, for reasonable employment agricultural chemicals, formulation resistance management scheme provide foundation comparatively accurately.
Blow cotton a red-spotted lizard biological activity determination and evaluation of pesticide effectiveness method outside simulating chamber of the present invention, comprise the following steps:
A. try worm and naturally inoculate raising: contain aspire to field acquisition occurrence injury serious band worm branches and leaves age blowing cotton a red-spotted lizard, band worm branches and leaves are docked on potted plant nursery stock to be tested;
B. make up a prescription: liquid reagent agent being mixed with setting concentration;
C. medicine is soaked: be soaked in the liquid prepared by whole for the band worm branches and leaves of potted plant nursery stock;
D. indoor feeding: the potted plant nursery stock of dipped medicament is placed on light dark period L:D=14: 10h, temperature is 25 ± 1 DEG C, and relative humidity is raise in the phytotron of 75%;
E. result inspection: raise and to check tested death of blowing cotton a red-spotted lizard after 24h and survive situation, record its death toll and survive number;
Further, in step a, worm branches and leaves will be with by colligation on potted plant nursery stock, and then potted plant nursery stock be moved into airtight solarium and carry out nature inoculation raising;
Further, in step c, the soak time of described band worm branches and leaves is 10 seconds.
The present invention also discloses and blows cotton a red-spotted lizard biological activity determination and the application of evaluation of pesticide effectiveness method in plant protection art outside a kind of simulating chamber.
Beneficial effect of the present invention: blow cotton a red-spotted lizard biological activity determination and evaluation of pesticide effectiveness method outside a kind of simulating chamber of the present invention, band worm branches and leaves are utilized to carry out nature inoculation by bind in the mode of nursery stock to be tested, survival rate is higher, and do not damage examination worm, live plant is adopted to cultivate the examination worm soaking liquid, ensure to blow the fresh of cotton a red-spotted lizard food and the nutrient that abundance is provided for blowing cotton a red-spotted lizard, tested by the simulation outdoor elements growth conditions blown residing for cotton a red-spotted lizard, the accuracy of effective raising bioassay results and accuracy, thus cotton a red-spotted lizard population resistance level is blown in comparatively comprehensively accurate and accurate understanding, for reasonable employment agricultural chemicals, formulate resistance management scheme and provide foundation comparatively accurately.
Embodiment
Blow cotton a red-spotted lizard biological activity determination and evaluation of pesticide effectiveness method outside the simulating chamber of the present embodiment, comprise the following steps:
A. try worm and naturally inoculate raising: contain aspire to field acquisition occurrence injury serious band worm branches and leaves age blowing cotton a red-spotted lizard, band worm branches and leaves are docked on potted plant nursery stock to be tested;
B. make up a prescription: liquid reagent agent being mixed with setting concentration;
C. medicine is soaked: be soaked in the liquid prepared by whole for the band worm branches and leaves of potted plant nursery stock;
D. indoor feeding: the potted plant nursery stock of dipped medicament is placed on light dark period L:D=14: 10h, temperature is 25 ± 1 DEG C, and relative humidity is raise in the phytotron of 75%;
E. result inspection: raise and to check tested death of blowing cotton a red-spotted lizard after 24h and survive situation, record its death toll and survive number; Adopt the band worm branches and leaves live plant leaching medicine the mode of raising that soak potted plant nursery stock, the self-sow environment blowing cotton a red-spotted lizard is createed with this, that gas penetration potential and institute's phytophagous freshness etc. are all with natural conditions and indifference, by the outer natural conditions of simulating chamber, accuracy and the accuracy of bioassay results is improved with this, thus cotton a red-spotted lizard population resistance level is blown in comparatively comprehensively accurate and accurate understanding, for reasonable employment agricultural chemicals, formulation resistance management scheme provide foundation comparatively accurately.
In the present embodiment, the soak time of described band worm branch is 10 seconds;
In the present embodiment, in step a, by band worm branches and leaves by upper in potted plant nursery stock of colligation, then potted plant nursery stock is moved into airtight solarium and carry out nature inoculation and raise.
By outside this simulating chamber blow cotton a red-spotted lizard biological activity determination and evaluation of pesticide effectiveness method is applied in plant protection art.
Below by specific embodiment, the present invention is further elaborated.
Embodiment one
1. try worm condition of culture
Blowing the cotton coccid egg hatching Sheng phase in mid-June (Chongqing June), the branch that field acquisition occurrence injury is serious, take back indoor, the branches and leaves of clip appropriate length, tie up and naturally inoculate on indoor pot nursery stock, then concentrate a large airtight solarium to carry out nature inoculation potted plant nursery stock to raise preparation and test, during test, select ceroplastes rubens and from generation to generation neat potted plant nursery stock branch occurs and blade is tested, reject inactive and generation irregular individuality, only retain and grow more neat individuality from generation to generation, then be dipped in the liquid of the variable concentrations prepared whole for band worm branch, dip time 10 seconds, the nursery stock of each medicament variable concentrations process is concentrated and saves, be convenient to the death toll of medicine " Invest, Then Investigate " aphid, then the potted plant nursery stock of dipped medicament being placed on light dark period is 14: 10h, (25 ± 1) DEG C, in the phytotron of relative humidity 75%, often process and repeat for 4 times, mortality ratio is checked after normal raising 24h, laboratory is gone back to the branch of the dipped medicament of scissors clip, mortality ratio is checked with pin.
2. reagent
2.1 test medicine
25%JS7119EW is provided by client
3% Acetamiprid
Common name: acetamiprid is provided by client
2.2 contrast medicaments
40% crystallization Rogor
Common name dimethoate Chongqing insecticide factory provides
2.3 other reagent
Dimethyl formamide (solvent)
Common name Dimethylformamid Chengdu Hong He chemical reagent factory
3 test process
3.1 dosage are arranged
3.2 tests repeat
Test arranges 4 repetitions, the insect population of each repetition more than 30.
3.3 processing mode
3.3.1 processing time and number of times
Adopt infusion process to process the cotton a red-spotted lizard that blows of raising, 24 hours " Invest, Then Investigate " death condition, carry out statistical study.
3.3.2 apparatus and administrated method is used
With graduated cylinder, first the apparatuses such as beaker will be diluted to the mother liquor of desired concn for the former medicine of examination, then dilute with water mother liquor is to the concentration preset, and application infusion process processes examination worm.
3.3.2 disposal route
Two medicaments are made into 5 ~ 7 dilute concentrations according to a certain percentage respectively, the best dilution intervals that reagent agent test is selected in pilot study is first done before test, soak immersing with the branch blowing cotton a red-spotted lizard in the liquid converted 10 seconds according to the concentration range that pilot study is selected, separately do 4 leaching clear water in contrast, then dipped potted plant nursery stock being placed on light dark period is 14: 10h, (25 ± 1) DEG C, in the phytotron of relative humidity 75%, often process and repeat for 4 times, mortality ratio is checked after normal raising 24h, laboratory is gone back to the branch of the dipped medicament of scissors clip, mortality ratio is checked with pin.Virulence regression equation, LC is calculated with probit analysis
50, standard error is equivalent.
4. computing method
Become by concentration conversion ppm to input computing machine (the irm software that plant protection institute of Agricultural University Of Southwest makes) later, obtain virulence regression equation, LC
50value, standard error SD, according to LC
50value and standard error calculate 95% confidence limit (see table 1).
5. evaluate
The LC of JS7119
50value is greater than contrast medicament and 3% Acetamiprid, illustrates that its activity is lower, and its b value is large compared with two contrast medicaments, and illustrate that degree of scatter is less, citrus icerya purchasi is more even to its reaction.
Table 125%JS7119EW and Acetamiprid blow cotton a red-spotted lizard indoor biological determination of activity regression equation, the lethal concentration of 50 and co-toxicity coefficient to citrus
Table 225%JS7119EW and Acetamiprid control citrus blow cotton a red-spotted lizard indoor biological determination of activity raw data table
What finally illustrate is, above embodiment is only in order to illustrate technical scheme of the present invention and unrestricted, although with reference to preferred embodiment to invention has been detailed description, those of ordinary skill in the art is to be understood that, can modify to technical scheme of the present invention or equivalent replacement, and not departing from aim and the scope of technical solution of the present invention, it all should be encompassed in the middle of right of the present invention.
Claims (4)
1. blow cotton a red-spotted lizard biological activity determination and an evaluation of pesticide effectiveness method outside simulating chamber, it is characterized in that:
Comprise the following steps:
A. try worm and naturally inoculate raising: contain aspire to field acquisition occurrence injury serious band worm branches and leaves age blowing cotton a red-spotted lizard, band worm branches and leaves are docked on potted plant nursery stock to be tested;
B. make up a prescription: liquid reagent agent being mixed with setting concentration;
C. medicine is soaked: be soaked in the liquid prepared by whole for the band worm branches and leaves of potted plant nursery stock;
D. indoor feeding: the potted plant nursery stock of dipped medicament is placed on light dark period L:D=14: 10h, temperature is 25 ± 1 DEG C, and relative humidity is raise in the phytotron of 75%;
E. result inspection: raise and to check tested death of blowing cotton a red-spotted lizard after 24h and survive situation, record its death toll and survive number.
2. blow cotton a red-spotted lizard biological activity determination and evaluation of pesticide effectiveness method outside simulating chamber according to claim 1, it is characterized in that: in step a, worm branches and leaves will be with by colligation on potted plant nursery stock, then potted plant nursery stock is moved into airtight solarium and carry out nature inoculation and raise.
3. blow cotton a red-spotted lizard biological activity determination and evaluation of pesticide effectiveness method outside simulating chamber according to claim 2, it is characterized in that: in step c, the soak time of described band worm branches and leaves is 10 seconds.
4. outside the simulating chamber described in any one of claims 1 to 3, blow cotton a red-spotted lizard biological activity determination and the application of evaluation of pesticide effectiveness method in plant protection art.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112034097A (en) * | 2019-07-31 | 2020-12-04 | 广西壮族自治区植保站 | Method for determining trapping and killing effect of prodenia litura adults |
Citations (3)
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| CN101653141A (en) * | 2009-09-17 | 2010-02-24 | 西华大学 | Application of 3,7,11-trimethyl-2,6,10-dodecatriene-1-ol in stellera chamaejasme to pesticides. |
| CN101999418A (en) * | 2010-11-30 | 2011-04-06 | 中南林业科技大学 | Plant source pesticide for preventing and controlling golden autumn pear diseases and insect pests and preparation and application thereof |
| CN104413038A (en) * | 2013-09-06 | 2015-03-18 | 沈阳化工研究院有限公司 | Binary insecticide composition and application |
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2014
- 2014-12-30 CN CN201410842556.5A patent/CN104535748A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101653141A (en) * | 2009-09-17 | 2010-02-24 | 西华大学 | Application of 3,7,11-trimethyl-2,6,10-dodecatriene-1-ol in stellera chamaejasme to pesticides. |
| CN101999418A (en) * | 2010-11-30 | 2011-04-06 | 中南林业科技大学 | Plant source pesticide for preventing and controlling golden autumn pear diseases and insect pests and preparation and application thereof |
| CN104413038A (en) * | 2013-09-06 | 2015-03-18 | 沈阳化工研究院有限公司 | Binary insecticide composition and application |
Non-Patent Citations (2)
| Title |
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| 刘浩强等: "昆虫生长调节剂对柑桔潜叶蛾的毒力测定及药效评价", 《西南师范大学学报（自然科学版）》 * |
| 刘浩强等: "炔螨特与哒螨灵不同配比对桔全爪螨室内毒力测定", 《中国南方果树》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112034097A (en) * | 2019-07-31 | 2020-12-04 | 广西壮族自治区植保站 | Method for determining trapping and killing effect of prodenia litura adults |
| CN112034097B (en) * | 2019-07-31 | 2022-06-07 | 广西壮族自治区植保站 | Method for determining trapping and killing effect of prodenia litura adults |
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