CN104655800A - Outdoor simulation citrus aphid biological activity and pesticide effect testing method and application thereof - Google Patents
Outdoor simulation citrus aphid biological activity and pesticide effect testing method and application thereof Download PDFInfo
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- CN104655800A CN104655800A CN201410840376.3A CN201410840376A CN104655800A CN 104655800 A CN104655800 A CN 104655800A CN 201410840376 A CN201410840376 A CN 201410840376A CN 104655800 A CN104655800 A CN 104655800A
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Abstract
The invention discloses an outdoor simulation citrus aphid biological activity and pesticide effect testing method and the application thereof. The method comprises the steps of preparing an insecticide for the test into liquid medicine with set concentration; soaking a whole branch with aphids of a potted nursery stock into the prepared liquid medicine, and then sealing and sleeving the soaked branch with the aphids by fine and soft gauze; putting the potted nursery stock soaked in the insecticide into a phytotron, and raising, wherein the light dark period L: D is equal to 14: 10 hours, the temperature is 25+/-1 DEG C, and the relative humidity is 75%; after raising for 24 hours, checking the death and survival conditions of the tested aphids, and recording the death number and the survival number; culturing the tested aphids soaked in the liquid medicine by a living plant, maintaining the food of the aphids to be fresh and providing enough nutrient for the aphids. According to the method, the aphids are tested under the condition that the natural growth environment conditions are simulated, and the aphids are prevented from escaping, so that the accuracy of biological assay results can be effectively improved, and a relatively accurate basis is provided for relatively comprehensively and accurately knowing the population resistance level of the aphids, reasonably using pesticides and formulating a resistance treatment scheme.
Description
Technical field
The present invention relates to insect pest of the plant prevention and control field, the citrus aphid biological activity determination particularly outside a kind of simulating chamber and evaluation of pesticide effectiveness method and application thereof.
Background technology
Aphid is the insect of orange young, be clustered on young sprout tender leaf with adult and nymph and suck juice, shrinkage curling by the young sprout tender leaf endangered, shortened internodes, can not normal extension, time serious, tender top drying is withered, and causes young fruit to come off and a large amount of young sprout cannot be extracted out, the not only underproduction then, also can affect lower annual production, " honeydew " of aphid excretion can bring out Sooty mould, affects leaf photosynthesis, citrus aphid or the communication media of citrus decline.Indoor bioassay be a kind of can more comprehensively, more accurately understand the means of aphid field population resistance level, also be a kind of method of comparatively traditional understanding resistance, now widely used various bioassay method, seedling method, drop method is soaked as being with leaf, to large for the damage of examination aphid, contrast mortality ratio is large, the raw accuracy surveying result of impact.The blade medicine embrane method of current use is a kind of ideal aphid bioassay method, but generally all use glass culture dish or batch cultur ware, airtight space, aphid are easily escaped, and it is large to take up room, accurately cannot determine the size of the blade that biologicall test is used, and be not all that the live plant adopted is cultivated examination worm, affect bioassay results accuracy.The method of aphid indoor bioassay disclosed in CN103675255, what it adopted is raised by rice paper sealing after blade leaching medicine, blade is from strain blade, the nutrient loss in the freshness of blade and blade cannot be held, there is very big-difference in its feeding environment and aphid self-sow environment, and though rice paper sealing can solve the problem of escaping, but its gas penetration potential is still not good, be unable to catch up with the aphid gas penetration potential environment under natural conditions far away, therefore cause the accuracy poor effect of its bioassay results, degree of accuracy is not high.
Summary of the invention
In view of this, the object of the present invention is to provide citrus aphid biological activity determination outside a kind of simulating chamber and evaluation of pesticide effectiveness method and application thereof, live plant is adopted to cultivate the examination worm soaking liquid, ensure the fresh of aphid food and provide sufficient nutrient for aphid, test under the physical environment growth conditions residing for aphid, aphid can be avoided again to escape simultaneously, the accuracy of effective raising bioassay results and accuracy, thus comparatively comprehensively accurate and accurate understanding aphis population resistance level, for reasonable employment agricultural chemicals, formulate resistance management scheme and provide foundation comparatively accurately.
Citrus aphid biological activity determination outside simulating chamber of the present invention and evaluation of pesticide effectiveness method, comprise the following steps:
A. make up a prescription: liquid reagent agent being mixed with setting concentration;
B. medicine is soaked: be soaked in the liquid prepared by whole for the band worm branch of nursery stock, the soft gauze big envelope of band worm branch then will soaked;
C. indoor feeding: the potted plant nursery stock of dipped medicament is placed on light dark period L:D=14: 10h, temperature is 25 ± 1 DEG C, and relative humidity is raise in the phytotron of 75%;
D. result inspection: check the death of tested aphid after raising 24h and survive situation, records its death toll and survives number;
Further, the soak time of described band worm branch is 10 seconds;
Further, also comprise examination worm to cultivate: before aphid occurrence in peak period, outdoor potted plant nursery stock is put in the middle of land for growing field crops, take the method that nature is inoculated, make nursery stock branch infect aphid in a large number, test after then concentrating a large airtight solarium to carry out natural feeding 2 generation the potted plant nursery stock having infected aphid;
Further, described nursery stock is the annual above potted plant nursery stock with spring tip branch then;
(at the beginning of 3 months, being concentrated by potted plant for the outdoor of more than 1 year nursery stock pruning branch to put the tip be put in the middle of land for growing field crops)
The present invention also discloses citrus aphid biological activity determination outside a kind of simulating chamber and the application of evaluation of pesticide effectiveness method in plant protection art.
Beneficial effect of the present invention: the citrus aphid biological activity determination outside a kind of simulating chamber of the present invention and evaluation of pesticide effectiveness method and application thereof, live plant is adopted to cultivate the examination worm soaking liquid, ensure the fresh of aphid food and provide sufficient nutrient for aphid, carry out test by the self-sow environmental baseline simulating aphid by the self-sow environmental baseline simulating Unaspis yanonensis to test, aphid can be avoided again to escape simultaneously, the accuracy of effective raising bioassay results and accuracy, thus comparatively comprehensively accurate and accurate understanding aphis population resistance level, for reasonable employment agricultural chemicals, formulate resistance management scheme and provide foundation comparatively accurately.
Embodiment
Citrus aphid biological activity determination outside the simulating chamber of the present embodiment and evaluation of pesticide effectiveness method, comprise the following steps:
A. make up a prescription: liquid reagent agent being mixed with setting concentration;
B. medicine is soaked: be soaked in the liquid prepared by whole for the band worm branch of potted plant nursery stock, the soft gauze big envelope of band worm branch then will soaked;
C. indoor feeding: the potted plant nursery stock of dipped medicament is placed on light dark period L:D=14: 10h, temperature is 25 ± 1 DEG C, and relative humidity is raise in the phytotron of 75%;
D. result inspection: check the death of tested aphid after raising 24h and survive situation, records its death toll and survives number; Adopt the band worm branch live plant leaching medicine the mode of raising that soak potted plant nursery stock, and adopt the mode of soft gauze big envelope band worm branch to create the self-sow environment of aphid with this, that gas penetration potential and institute's phytophagous freshness etc. are all with natural conditions and indifference, accuracy and the accuracy of bioassay results is improved with this, thus comparatively comprehensively accurate and accurate understanding aphis population resistance level, for reasonable employment agricultural chemicals, formulation resistance management scheme provide foundation comparatively accurately.
In the present embodiment, the soak time of described band worm branch is 10 seconds;
In the present embodiment, also comprise examination worm to cultivate: before aphid occurrence in peak period, outdoor potted plant nursery stock is put in the middle of land for growing field crops, take the method that nature is inoculated, make potted plant nursery stock branch infect aphid in a large number, test after then concentrating a large airtight solarium to carry out natural feeding 2 generation the potted plant nursery stock having infected aphid.During test, there is neat potted plant nursery stock branch and test in the aphid selecting enough tests from generation to generation, rejects inactive and generation irregular individuality, only retain and grow more neat individuality from generation to generation.
In the present embodiment, described potted plant nursery stock is more than the 1 year nursery stock with spring tip branch then.
(at the beginning of 3 months, being concentrated by potted plant for the outdoor of more than 1 year nursery stock pruning branch to put the tip be put in the middle of land for growing field crops)
Citrus aphid biological activity determination outside this simulating chamber and evaluation of pesticide effectiveness method are applied in plant protection art.
Below by specific embodiment, the present invention is further elaborated.
Embodiment one
1. try worm condition of culture
Before citrus aphid occurrence in peak period (May is occurrence in peak period), outdoor potted plant nursery stock (more than life in 1 year, because aphid is liked endangering the tender tip, tender leaf, is taked at the beginning of 3 months.Potted plant nursery stock is concentrated and prunes branch, put once slightly) be put in the middle of land for growing field crops, take the method that nature is inoculated, potted plant nursery stock branch is made to infect aphid in a large number, then concentrate a large airtight solarium to carry out natural feeding the potted plant nursery stock having infected aphid to prepare to test, during test, there is neat potted plant nursery stock branch from generation to generation and test in the aphid selecting enough tests, reject inactive and generation irregular individuality, only retain and grow more neat individuality from generation to generation, then be dipped in the liquid of the variable concentrations prepared whole for band worm branch, dip time 10 seconds, the branch of each medicament variable concentrations process is got up with soft gauze net respectively, be convenient to the death toll of medicine " Invest, Then Investigate " aphid, then the potted plant nursery stock of dipped medicament being placed on light dark period is 14: 10h, (25 ± 1) DEG C, in the phytotron of relative humidity 75%, often process and repeat for 4 times, mortality ratio is checked after normal raising 24h, laboratory is gone back to the net branch of the dipped medicament of scissors clip, open net writing brush and stir polypide, can not stand if fallen down and namely be considered as death.
2. reagent
2.1 test medicine
The former medicine of 96.5% Acetamiprid
Common name: acetamiprid is provided by client
2.2 contrast medicaments
98% crystallization Rogor
Common name dimethoate Chongqing insecticide factory provides
2.3 other reagent
Dimethyl formamide (solvent)
Common name Dimethylformamid Chengdu Hong He chemical reagent factory
3 test process
3.1 dosage are arranged
3.2 tests repeat
Test arranges 4 repetitions, the insect population of each repetition more than 30.
3.3 processing mode
3.3.1 processing time and number of times
Adopt infusion process to process the aphid of raising, 24 hours " Invest, Then Investigate " death condition, carry out statistical study.
3.3.2 apparatus and administrated method is used
With graduated cylinder, first the apparatuses such as beaker are diluted to for the former medicine of examination the mother liquor that desired concn is 3% Acetamiprid, 40% Rogor, and then dilute with water mother liquor is to the concentration preset, and application infusion process processes examination worm.
3.3.2 disposal route
Two medicaments are made into 5 ~ 7 dilute concentrations according to a certain percentage respectively, the best dilution intervals that reagent agent test is selected in pilot study is first done before test, branch with aphid immerses in the liquid converted and soaks 10 seconds by the concentration range selected according to pilot study, separately do 4 leaching clear water in contrast, then dipped potted plant nursery stock being placed on light dark period is 14: 10h, (25 ± 1) DEG C, in the phytotron of relative humidity 75%, often process and repeat for 4 times, mortality ratio is checked after normal raising 24h, laboratory is gone back to the net branch of the dipped medicament of scissors clip, open net writing brush and stir polypide, can not stand if fallen down and namely be considered as death.Virulence regression equation, LC is calculated with probit analysis
50, standard error is equivalent.
4. computing method
Become by concentration conversion ppm to input computing machine (the irm software that plant protection institute of Agricultural University Of Southwest makes) later, obtain virulence regression equation, LC
50value, standard error SD, according to LC
50value and standard error calculate 95% confidence limit (see table 1).
5. evaluate
The LC of Acetamiprid
50value is less than contrast medicament Rogor, illustrates that its activity is higher, and its b value is little compared with Rogor, SE value is comparatively large, and illustrate that degree of scatter is comparatively large, citrus aphid is larger to its response difference.
Table 1 Acetamiprid is to citrus aphid indoor biological determination of activity regression equation, the lethal concentration of 50 and co-toxicity coefficient
Table 2 Trials of Acetamiprid EC against Citrus Aphides indoor biological determination of activity raw data table
What finally illustrate is, above embodiment is only in order to illustrate technical scheme of the present invention and unrestricted, although with reference to preferred embodiment to invention has been detailed description, those of ordinary skill in the art is to be understood that, can modify to technical scheme of the present invention or equivalent replacement, and not departing from aim and the scope of technical solution of the present invention, it all should be encompassed in the middle of right of the present invention.
Claims (5)
1. the citrus aphid biological activity determination outside simulating chamber and an evaluation of pesticide effectiveness method, is characterized in that: comprise the following steps:
A. make up a prescription: liquid reagent agent being mixed with setting concentration;
B. medicine is soaked: be soaked in the liquid prepared by whole for the band worm branch of nursery stock, the soft gauze big envelope of band worm branch then will soaked;
C. indoor feeding: the nursery stock of dipped medicament is placed on light dark period L:D=14: 10h, temperature is 25 ± 1 DEG C, and relative humidity is raise in the phytotron of 75%;
D. result inspection: check the death of tested aphid after raising 24h and survive situation, records its death toll and survives number.
2. the citrus aphid biological activity determination outside simulating chamber according to claim 1 and evaluation of pesticide effectiveness method, is characterized in that: the soak time of described band worm branch is 10 seconds.
3. the citrus aphid biological activity determination outside simulating chamber according to claim 2 and evaluation of pesticide effectiveness method, it is characterized in that: also comprise examination worm and cultivate: before aphid occurrence in peak period, nursery stock is put in the middle of land for growing field crops, take the method that nature is inoculated, make potted plant nursery stock branch infect aphid in a large number, test after then concentrating a large airtight solarium to carry out natural feeding 2 generation the potted plant nursery stock having infected aphid.
4. the citrus aphid biological activity determination outside simulating chamber according to claim 3 and evaluation of pesticide effectiveness method, is characterized in that: described nursery stock is the annual above potted plant nursery stock with spring tip branch then.
5. the citrus aphid biological activity determination outside the simulating chamber described in any one of Claims 1 to 4 and the application of evaluation of pesticide effectiveness method in plant protection art.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN112790161A (en) * | 2020-12-30 | 2021-05-14 | 中国农业大学 | Indoor biological assay method for myzus persicae |
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| CN112790161A (en) * | 2020-12-30 | 2021-05-14 | 中国农业大学 | Indoor biological assay method for myzus persicae |
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