CN106069740A - A kind of screening salt tolerance Jerusalem artichoke method - Google Patents
A kind of screening salt tolerance Jerusalem artichoke method Download PDFInfo
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- CN106069740A CN106069740A CN201610499511.1A CN201610499511A CN106069740A CN 106069740 A CN106069740 A CN 106069740A CN 201610499511 A CN201610499511 A CN 201610499511A CN 106069740 A CN106069740 A CN 106069740A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/04—Processes of selection involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection
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Abstract
The invention provides a kind of method screening salt tolerance Jerusalem artichoke.In the inventive method, by with Jerusalem artichoke tuber as raw material, use the method that comparison is cultivated simultaneously, and it is analyzed statistics according to the various trait parameter of Jerusalem artichoke growth further, thus draw the salt tolerance index of variety classes Jerusalem artichoke, and filter out the species of jeruselem artichoke with different salt tolerance, and there is breeding method easy, screening technique science, efficiency is high and the result advantage such as accurately.
Description
Technical field
The present invention relates to salt tolerance of crop screening field, screen salt tolerance Jerusalem artichoke in particular to one.
Background technology
In soil, too high in salinity can affect crop growth, and this be also cause crop production reduction main abiotic because of
Element.At present, whole world tilth salinization day by day serious, and the growth of crops and the production of peasant household and life are also brought by this
The biggest impact.
Jerusalem artichoke is one perennial root herbaceous plant for many years, has good drought resisting tolerance to cold and drought resisting anti-blown sand.Chrysanthemum
The fructose polymers such as the underground stem block rich in starch of taro bulk and inulin, it is also possible to be further used for producing starch and ethanol is former
Material.Meanwhile, Jerusalem artichoke also has good anti-blown sand ability and stronger vitality, additionally it is possible to sand ground is carried out soil improvement.Cause
This, Jerusalem artichoke is also a kind of to be very suitable for the crop at vast desert popularizing planting.
Owing to soil, Desert Area saline and alkaline is higher, therefore, filter out and there is more preferable salt tolerance species of jeruselem artichoke for Jerusalem artichoke
Popularizing planting and further desert transformation be all the most significant.But, existing Jerusalem artichoke salt tolerance evaluating method
It is all with Jerusalem artichoke in seedling stage for testing raw material and using the method in the time of infertility to carry out, but, these methods need substantial amounts of former
Expecting and the longest, the plantation repeatedly and the detection that generally require many seasons just can draw effective result.
Therefore, develop and a kind of the method for rapid screening salt tolerance Jerusalem artichoke can the most just become current technology urgently to be resolved hurrily
Problem.
In view of this, the special proposition present invention.
Summary of the invention
The first object of the present invention is to provide a kind of method screening salt tolerance Jerusalem artichoke, in described method, by with
Jerusalem artichoke tuber is raw material, uses the method that comparison is cultivated simultaneously, and carries out according to the various trait parameter of Jerusalem artichoke growth further
Analytic statistics, thus draw the salt tolerance index of variety classes Jerusalem artichoke, and filter out the species of jeruselem artichoke with different salt tolerance, enter
And time-consumingly long etc. technical problem can be solved the while that prior art screening technique needing material many.The inventive method has cultivation
The advantages such as method simplicity, screening technique science, efficiency height and result are accurate.
In order to realize the above-mentioned purpose of the present invention, spy by the following technical solutions:
A kind of method screening salt tolerance Jerusalem artichoke, described method comprises the steps: to choose variety classes and eye is not subject to
The Jerusalem artichoke tuber of injury, cuts various Jerusalem artichoke tuber respectively and obtains organizing different types of Jerusalem artichoke kind that eye quantity is consistent more
Block；
Different types of Jerusalem artichoke kind block is planted respectively, to part Jerusalem artichoke kind block nutrient solution in each group as right
According to group；Meanwhile, the add identical nutritional solution of sodium chloride extra to the pouring of remaining Jerusalem artichoke kind block is as experimental group, and trains simultaneously
Educate；
The property such as width that after cultivating each group respectively, the Jerusalem artichoke plant of gained carries out plant height, root is heavy, stem weight, leaf are heavy and leaf comes into leaves
The parameter of shape is measured, and is contrasted with each character parameter of plant in experimental group by matched group in each group of cultivation group respectively
Statistical analysis, obtains the relevance parameter between the parameter relative value of each character, each character and can embody the master of Jerusalem artichoke salt tolerance
Want character；
Relevance parameter between the major traits of the embodied Jerusalem artichoke salt tolerance drawn according to analytic statistics and each character with
And each character parameter relative value, draw the salt tolerance index of variety classes Jerusalem artichoke, and filter out the Jerusalem artichoke with different salt tolerance
Kind.
In the inventive method, by with Jerusalem artichoke tuber as raw material, use the method that comparison is cultivated, and basis further simultaneously
The various trait parameter of Jerusalem artichoke growth is analyzed statistics, thus calculates the salt tolerance index of variety classes Jerusalem artichoke, and sieves
Select the species of jeruselem artichoke with different salt tolerance, there is breeding method easy, screening technique science, efficiency is high and result accurately etc.
Advantage.
Optionally, in the present invention, described different types of Jerusalem artichoke is 12 kinds of Jerusalem artichokes, is specially blue or green taro No. 1, blue or green taro No. 2, green grass or young crops
Taro No. 3, corridor taro No. 1 and Xuzhou, Shanxi Lvliang City, Xinyu, Jiangxi, Huanggang, Lanzhou, Gansu, Jingning, Gansu and Shandong
Local species of jeruselem artichoke such as 8, Weifang etc..
Optionally, in the present invention, described cultivation is carried out in indoor.
Optionally, in the present invention, after respectively each group being cultivated the Jerusalem artichoke plant of gained carry out plant height, root fresh weight and dry weight,
Stem fresh weight and dry weight, leaf fresh weight and dry weight, the parameter of the character of the length and width of 2 leaves are measured.
Optionally, in the present invention, still further comprise the Jerusalem artichoke plant of gained after respectively each group being cultivated carry out germination percentage,
The statistics of the shape such as germinating energy and germination index or calculating.
Optionally, in the present invention, described method is additionally included in and cuts various Jerusalem artichoke tuber respectively and obtain organizing eye number more
After measuring consistent different types of Jerusalem artichoke kind block, Jerusalem artichoke tuber sterilized and rinses, then carrying out the step planted.
Optionally, in the present invention, described sterilization is with HgCl that concentration is 0.05～0.15%2Clean Jerusalem artichoke tuber to carry out
Sterilization；Preferably, in the present invention, described sterilization is with HgCl that concentration is 0.08～0.10%2Clean Jerusalem artichoke tuber to disappear
Poison.
In the present invention, by sterilization solvent for use and the adjustment of solvent strength and optimization, such that it is able to realize right
While Jerusalem artichoke tuber sufficiently sterilised, also will not be too high and Jerusalem artichoke tuber is brought damage due to sterilization reagent concentration.
Optionally, in the present invention, the time of described sterilization is 5～15min；Preferably, in the present invention, described sterilization time
Between be 8～12min, can be such as 9,10 or 11min etc..
In the present invention, by the selection of disinfecting time and adjustment such that it is able to while Jerusalem artichoke tuber sufficiently sterilised,
Also will not be due to long the caused damage to Jerusalem artichoke tuber of disinfecting time and the reduction of overall screening efficiency.
Optionally, in the present invention, described nutritional solution is 1/2Hogland nutritional solution.
In the present invention, by nutrient solution used selection and adjustment, and select the nutritional solution with abundant mineral, from
And ensure that the abundant growth of Jerusalem artichoke.
Optionally, in the present invention, in described nutritional solution, the concentration of the extra sodium chloride added is 100～180mM；Preferably
, in the present invention, in described nutritional solution, the concentration of the extra sodium chloride added is 120～150mM.
In the present invention, by the selection of added sodium chloride concentration and adjustment, thus can Validity Test and screening resistance to
While salt Jerusalem artichoke, also will not due to too high the caused Jerusalem artichoke of added sodium chloride concentration cannot normal growth.
Optionally, in the present invention, described cultivation is carried out under the conditions of illumination alternate cycle 12h/12h, the most described training
Educate is to carry out under the alternation condition of each 12h of illumination and dark.
In the present invention, by the selection of illumination condition in cultivating process and adjustment, so that Jerusalem artichoke can enter
One step improves the speed of growth of Jerusalem artichoke.
Optionally, in the present invention, the ambient temperature of described cultivation is 15～25 DEG C, and ambient humidity is 40～60%；Preferably
, in the present invention, the temperature of described cultivation is 18～22 DEG C, and ambient humidity is 45-55%；It is furthermore preferred that in the present invention, at light
Under the conditions of according to, the temperature of described cultivation is 20～22 DEG C, and under dark condition, the temperature of described cultivation is 18～19 DEG C, cultivates
During the humidity of environment be 50%.
In the present invention, by the temperature cultivated and the selection of the condition such as humidity and adjustment such that it is able to carry further
The speed of growth of high Jerusalem artichoke.
Optionally, in the present invention, the time of described cultivation is at the beginning of the Jerusalem artichoke kind block after plantation is sent out after bud, then cultivate 12～
20d；Preferably, in the present invention, after the time of described cultivation is bud at the beginning of the Jerusalem artichoke kind block after plantation is sent out, then cultivate 14～16d.
In the present invention, by cultivating the selection of time and adjustment such that it is able to effectively Jerusalem artichoke is being carried out salt tolerance
While test and screening, additionally it is possible to avoid the overall screening efficiency caused due to cultivation overlong time too low.
Optionally, in the present invention, bud at the beginning of described Jerusalem artichoke kind block is sent out is that first bud of Jerusalem artichoke kind block exposes, and exposes cultivation with bud
Stromal surface 1cm used by kind is standard.
Optionally, in the present invention, it is molten that described method also includes watering salt in plantation substrate used in experimental group further
The step of liquid.
In the present invention, by watering saline solution in plantation substrate used, thereby may be ensured that saline solution fully permeates base
Matter.
Optionally, in the present invention, the described saline solution that waters in plantation substrate used is that pouring to saline solution just separates out.
Compared with prior art, the invention have the benefit that
(1) in the inventive method, by with Jerusalem artichoke tuber as raw material, and carry out according to the various trait parameter of Jerusalem artichoke growth
Analytic statistics, thus filter out the species of jeruselem artichoke with different salt tolerance, there is breeding method easy, screening technique science, effect
Rate is high and the result advantage such as accurately.
(2) in the inventive method, by cultivating adjustment and the optimization of the breeding conditions such as temperature and time, thus effectively
Jerusalem artichoke is carried out salt tolerance test and screening while, additionally it is possible to avoid owing to cultivating the overall screening that overlong time is caused
Efficiency is too low.
Accompanying drawing explanation
Fig. 1 is Jerusalem artichoke salt tolerance Cluster Classification figure.
Detailed description of the invention
Below in conjunction with embodiment, embodiment of the present invention are described in detail, but those skilled in the art will
Understanding, the following example is merely to illustrate the present invention, and is not construed as limiting the scope of the present invention.In embodiment unreceipted specifically
Condition person, the condition advised according to normal condition or manufacturer is carried out.Agents useful for same or instrument unreceipted production firm person, be
Can be by the commercially available conventional products bought and obtain.
Embodiment 1
12 kinds of Jerusalem artichokes, are specially blue or green taro No. 1, blue or green taro No. 2, blue or green taro No. 3, Xuzhou local varieties 1, ground, Xuzhou
Side kind 2, Shanxi Lvliang City local varieties, corridor taro No. 1, Xinyu, Jiangxi local varieties, Huanggang local varieties, Lanzhou, Gansu
Local varieties, Jingning, Gansu local varieties and Weifang, Shandong kind, be denoted as 1-12 group respectively.
Choose the Jerusalem artichoke tuber of the 1-12 group that eye is not injured respectively, clean with distilled water flushing, cut 1-the most respectively
The Jerusalem artichoke tuber of 12 groups, obtains organizing the Jerusalem artichoke kind block of the consistent 1-12 group of eye quantity more, and concentration is 0.10%
HgCl2After solution is respectively to the Jerusalem artichoke kind block cleaning and sterilizing 10min of obtained 1-12 group cleaner with distilled water flushing.
Being inoculated into respectively by the Jerusalem artichoke kind block of the 1-12 group rinsed well equipped with in 15 hole dishes of quartz sand, every hole is inoculated
1 part of Jerusalem artichoke kind block, every kind of Jerusalem artichoke kind block 6 hole tray of inoculation.Then, 3 caves in watering each group with 1/2Hogland nutritional solution
Dish, and as a control group；Meanwhile, remaining 3 cave during the 1/2Hogland nutritional solution with NaCl concentration as 125mM waters each group
Dish, and as experimental group.
Then, in indoor environment, periodicity of illumination 12h/12h, illumination temperature is 22 DEG C, dark illumination temperature is 18 DEG C,
Under conditions of indoor humidity 50%, each group of Jerusalem artichoke kind block is cultivated.
Then, when each group Jerusalem artichoke first bud of kind block self-discovery exposes, add up the germinative number of each group, and continue every day
Cultivate 14 days.Then at the 14th day, each hole tray often organized randomly selects 2 strain Jerusalem artichoke plant, and measures each group of chrysanthemum respectively
The length and width of the plant height of taro plant, root fresh weight and dry weight, stem fresh weight and dry weight, leaf fresh weight and dry weight, 2 leaves, wherein, sends out
Bud exposes stromal surface 1cm as standard with bud.
Meanwhile, calculate respectively and 1-12 group is respectively organized experimental group and the germination percentage of matched group Jerusalem artichoke kind block and germinating energy；Its
In, germination percentage=14th day germination Jerusalem artichoke kind block number/Jerusalem artichoke kind block total quantity × 100%, germinating energy=4th day germination Jerusalem artichoke kind
Block number/Jerusalem artichoke kind block total quantity × 100%, germination index (GI)=∑ (Gt/Dt), wherein Gt is t day chitting piece number,
Dt is the number of days that germinates accordingly.Then cultivate, to each group, each character of Jerusalem artichoke plant and germination percentage obtained and germinating energy enters
Row statistics, and calculate the meansigma methods of each group of each character of Jerusalem artichoke, it being designated as tree characteristics statistical value, result is as shown in table 1 below:
Table 1 1-12 group Jerusalem artichoke experimental group and matched group tree characteristics statistical value
Wherein, CK is the matched group in 1-12 group, and S is the experimental group in 1-12 group.
But, due to the difference of each group of Jerusalem artichoke kind, thus the system of the statistical value (or absolute value) of each character of Jerusalem artichoke in table 1
Meter meaning is the most little.Thus, further with the relative value of each characteristic index, i.e. the ratio of experimental group/matched group is as statistics
Analysis foundation data, and this salt tolerance also being able to preferably reflect different species of jeruselem artichoke.
According to each character statistical value in table 1 1-12 group Jerusalem artichoke experimental group and matched group tree characteristics, calculate each further
The statistics relative value (i.e. the ratio * 100 of experimental group/matched group) of the group each character of Jerusalem artichoke, and statistic record, result such as table 2 below institute
Show:
Table 2 1-12 group Jerusalem artichoke experimental group and matched group tree characteristics statistics relative value
Wherein, in table 2, after numeral, letter representation 5% level difference is the most notable.
According to the statistics relative value of each character of Jerusalem artichoke in table 2, carry out correlation analysis between character, analysis result further
As shown in table 3:
Table 3 each character correlation coefficient
Wherein, * and * * represents the significant level of 5% and 1% respectively
From correlation coefficient in table 3, although have employed statistics relative value and the statistical method of correlation coefficient, but
Owing to Germination characteristics under the conditions of same concentrations saline solution of the Jerusalem artichoke of different cultivars and growth characteristics data are more discrete.In order to
Preferably analyze the impact of each character pair Jerusalem artichoke salt tolerance, further with the principal component analysis in Chemical Measurement with fuzzy
Clustering method is analyzed, to discrete data standardization.
List correlation matrix according to correlation coefficient, and calculate characteristic root and corresponding characteristic vector；Further,
The character factor that interference is bigger in order to excretion is less, and improve the accuracy rate of analysis, from 12 characteristic roots, select 3
Bigger characteristic root and corresponding characteristic vector, and be further used as major influence factors and be analyzed, picked out main one-tenth
Divide (principal element on the impact of Jerusalem artichoke salt tolerance) and corresponding contribution rate thereof, as shown in table 4:
Table 4 main constituent and contribution rate
| Main constituent | Initial characteristic values | Contribution rate (%) | Accumulation contribution rate (%) |
| 1 | 5.644 | 47.029 | 47.029 |
| 2 | 2.220 | 18.498 | 65.528 |
| 3 | 1.965 | 16.373 | 81.901 |
From table 4 main constituent and contribution rate, the accumulation contribution rate of three kinds of main constituents can reach more than 80%, and this is sufficient
To show the variation tendency of Jerusalem artichoke plant salt tolerance, comply fully with the requirement of principal component analysis, so taking this three kinds of component conducts
The effective ingredient of data analysis.
Meanwhile, the relative coefficient of 3 main constituents of computational analysis and all character statistics relative value, result such as table 5 below institute
Show:
Table 5 main constituent and all characters correlation coefficients
1st main constituent can explain the change of 11 salt-tolerance characters 47.029%, and main reflection is that overground part grows shape
Condition, its with plant height, the correlation coefficient of stem fresh weight, leaf fresh weight, stem weight and leaf dry weight is mostly more than 0.8.
2nd main constituent has bigger positive correlation with root fresh weight and root dry weight, the main root growth reflecting Jerusalem artichoke
Situation.
3rd main constituent is with the factor loading of each index, and the loading with germination percentage is maximum, is 0.681, secondly for germinateing
Index and germinating energy, mainly reflect the sprouting state of Jerusalem artichoke.
Then, add up relative value's correlative relationship according to 3 main constituents with all character, calculate 3 main constituents
Scoring systems matrix, result is as shown in table 6 below
6 three principal component scores sytem matrixes of table
Scoring systems matrix according to 3 main constituents, can obtain the score formula of each factor:
F1=0.089X1+0.128X2+0.111X3+0.143X4+0.069X5+0.149X6+0.148 X7+0.076X8+
0.139X9+0.146X10+0.104X11+0.119X12；
F2=-0.158X1-0.077X2-0.050X3-0.114X4+0.392X5-0.069X6+0.14 8X7+0.389X8-
0.075X9+0.146X10-0.165X11-0.135X12；
F3=0.347X1+0.293X2+0.334X3-0.081X4+0.056X5-0.008X6-0.138 X7+0.055X8-
0.010X9-0.132X10-0.258X11-0.275X12；
According to the value of F1, F2 and F3, calculate the comprehensive salt tolerance score formula of each kind, salt tolerant score F=further
0.47029F1+0.18498F2+0.16373F3。
Further, by calculating the salt tolerant score F value of each group of Jerusalem artichoke, 12 groups of Jerusalem artichokes are carried out salt tolerance sequence, result
As shown in table 7 below:
Table 7 each kind Jerusalem artichoke salt tolerance sorts
| Kind | F1 | F2 | F3 | F | Salt tolerance sorts |
| 1 | 109.97 | 38.82 | 60.92 | 68.87 | 1 |
| 2 | 61.87 | -10.18 | 50.47 | 35.48 | 9 |
| 3 | 68.01 | -25.58 | 36.57 | 33.24 | 10 |
| 4 | 59.33 | -2.70 | 26.56 | 31.75 | 11 |
| 5 | 104.85 | 33.56 | 30.93 | 60.58 | 2 |
| 6 | 97.81 | -16.21 | 19.32 | 46.16 | 5 |
| 7 | 109.35 | -28.40 | -2.73 | 45.73 | 6 |
| 8 | 45.82 | -9.87 | -8.90 | 18.27 | 12 |
| 9 | 86.90 | -45.35 | 68.92 | 43.76 | 7 |
| 10 | 106.75 | -44.20 | 31.56 | 47.19 | 4 |
| 11 | 88.65 | -31.81 | 15.36 | 38.32 | 8 |
| 12 | 108.77 | -39.39 | 41.36 | 50.64 | 3 |
Further, utilize averaging method between the class of systematic analytic method, species of jeruselem artichoke is carried out Cluster Classification, classification results
As it is shown in figure 1, and 12 species of jeruselem artichoke are divided into 4 big classes.Concrete, the 1st, 5 groups of Jerusalem artichokes are the 1st class, and this group has best
Salt tolerance；6th, 7,9,10,12 groups is the 2nd class, and this group salt tolerance is slightly worse；2nd, 3,4,11 is the 3rd group, and this group salt tolerance is relatively
Difference；8th group is the 4th group, and this group salt tolerance is worst.
In the inventive method, by with Jerusalem artichoke tuber as raw material, and carry out point according to the various trait parameter of Jerusalem artichoke growth
Analysis statistics, thus filter out the species of jeruselem artichoke with different salt tolerance, there is breeding method easy, screening technique science, efficiency
The advantages such as high and result is accurate
Although illustrate and describing the present invention with specific embodiment, but it will be appreciated that without departing substantially from the present invention's
May be made that in the case of spirit and scope many other change and amendment.It is, therefore, intended that in the following claims
Including all such changes and modifications belonged in the scope of the invention.
Claims (10)
1. the method screening salt tolerance Jerusalem artichoke, it is characterised in that described method comprise the steps: to choose variety classes and
Eye unharmed Jerusalem artichoke tuber, cuts various Jerusalem artichoke tuber obtaining respectively and organizes consistent different types of of eye quantity more
Jerusalem artichoke kind block；
Different types of Jerusalem artichoke kind block is planted respectively, to part Jerusalem artichoke kind block nutrient solution in each group as comparison
Group；Meanwhile, the add identical nutritional solution of sodium chloride extra to the pouring of remaining Jerusalem artichoke kind block is as experimental group, and trains simultaneously
Educate；
Come into leaves wide character parameter respectively to the plant height of Jerusalem artichoke plant of gained, root weight, stem weight, leaf weight and leaf after each group of cultivation
It is measured, and carries out contrasting statistical with each character parameter of plant in experimental group by matched group in each group of cultivation group respectively
Analysis, obtains the relevance parameter between the parameter relative value of each character, each character and can embody the major traits of Jerusalem artichoke salt tolerance；
Relevance parameter between the major traits of the embodied Jerusalem artichoke salt tolerance drawn according to analytic statistics and each character and each
Character parameter relative value, draws the salt tolerance index of variety classes Jerusalem artichoke, and filters out the species of jeruselem artichoke with different salt tolerance.
Method the most according to claim 1, it is characterised in that described method also includes cutting various Jerusalem artichoke tuber respectively also
After obtaining organizing different types of Jerusalem artichoke kind block that eye quantity is consistent more, Jerusalem artichoke tuber is sterilized and rinses, then carry out planting
Step.
Method the most according to claim 2, it is characterised in that described sterilization is with HgCl that concentration is 0.05～0.15%2
Clean Jerusalem artichoke tuber to carry out disinfection.
Method the most according to claim 3, it is characterised in that the time of described sterilization is 5～15min.
Method the most according to claim 1, it is characterised in that described nutritional solution is 1/2Hogland nutritional solution.
Method the most according to claim 1, it is characterised in that in described nutritional solution, the concentration of the extra sodium chloride added is
100～180mM.
Method the most according to claim 1, it is characterised in that described cultivation is under the conditions of illumination alternate cycle 12/12h
Carry out.
Method the most according to claim 7, it is characterised in that the ambient temperature of described cultivation is 15～25 DEG C, environmental wet
Degree is 40～60%.
Method the most according to claim 1, it is characterised in that the time of described cultivation is at the beginning of the Jerusalem artichoke kind block after plantation is sent out
After bud, then cultivate 12～20d.
Method the most according to claim 1, it is characterised in that described method also includes planting in experimental group further
Substrate used is watered the step of saline solution.
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