CN112022991A

CN112022991A - Fumigation liquid for yin deficiency and damp heat type xerophthalmia and preparation method thereof

Info

Publication number: CN112022991A
Application number: CN202011137085.XA
Authority: CN
Inventors: 翁晔; 楼何美子; 韩姝阳; 孟舒扬; 裘生梁; 刘艳莉; 熊阳; 王越颐; 李若淇; 杜郑雨桐; 李澳乐; 朱轶
Original assignee: Zhejiang Chinese Medicine University ZCMU
Current assignee: Zhejiang Chinese Medicine University ZCMU
Priority date: 2020-10-22
Filing date: 2020-10-22
Publication date: 2020-12-04

Abstract

The invention discloses a fumigating liquid for xerophthalmia caused by yin deficiency and damp-heat and a preparation method thereof, belongs to the technical field of traditional Chinese medicine extraction, and relates to a fumigating liquid for xerophthalmia caused by yin deficiency and damp-heat and a preparation method thereof. Then pulverizing respectively with Chinese medicinal material pulverizer, and sieving with 40 mesh sieve. Then the obtained Chinese medicinal powder is put into a container to be fully mixed and then is put into an extraction kettle of a supercritical device. In the extraction process, a composite modifier consisting of ethanol with the concentration of 80vt percent 300ml and Tween 80 with the concentration of 10ml is added through a liquid adding pump, after a period of extraction, extract liquor is collected from a separation kettle I and a separation kettle II and then is mixed and shaken evenly, which can increase local water liquid, is also beneficial to local vasodilatation, promotes blood circulation, and is beneficial to local nutrition supply and inflammation elimination.

Description

Fumigation liquid for yin deficiency and damp heat type xerophthalmia and preparation method thereof

Technical Field

The invention relates to the technical field of traditional Chinese medicine extraction, and particularly relates to a fumigating liquid for yin deficiency and damp heat type xerophthalmia and a preparation method thereof.

Background

Dry eye syndrome (dry-eye syndrome), also known as keratoconjunctival xerosis (KCS), is a general term for a variety of diseases characterized by ocular surface disorders due to decreased tear film stability caused by abnormalities in tear quality and quantity or kinetics, accompanied by ocular discomfort. Epidemiological and clinical studies have found that the incidence of dry eye is increasing. The western medicine treatment methods are various, wherein the tear substitution therapy is a main medicine for treating xerophthalmia at present due to convenient use, definite curative effect and small side effect, but no artificial tear liquid can completely accord with the components of the tear, and the preservative in the artificial tear, especially benzalkonium chloride, has certain damage to the ocular surface through research verification. Anti-inflammatory drugs are available for short-term treatment of dry eye, but are also associated with corneal epithelial toxicity. Androgens can maintain lacrimal glands and other ocular surface tissues in a non-inflammatory state, down-regulate immune activity, promote protein synthesis, have an amplifying effect on the lacrimal secretion process, and the like, but systemic application of sex hormones may cause more serious side effects. Surgical treatments are also not widely used due to the risks involved. Xerophthalmia due to yin deficiency and damp-heat is shown as follows: dryness, pain, blurred vision, thready and sticky eye gum, foul breath, constipation, dark and short urine, red or marginal tongue with teeth, yellowish or thick and greasy coating, and thready, soft and rapid pulse. The applicant obtains better curative effect for treating xerophthalmia by applying the traditional Chinese medicine in clinical practice for many years. However, the traditional method for extracting the effective components of the traditional Chinese medicine mainly comprises the following steps: impregnation, precooking, reflux extraction, steam distillation, distillation under reduced pressure, sublimation, etc. These methods have the disadvantages of complicated extraction process, low product purity, organic residue, and easy damage to heat-sensitive substances, which can also result in reduction of drug effect of the drugs. Therefore, the fumigation solution for treating yin deficiency and damp heat type xerophthalmia and the preparation method thereof are designed to solve the problems.

Disclosure of Invention

1. Technical problem to be solved

Aiming at the problems in the prior art, the first purpose of the invention is to provide the fumigating liquid for the yin deficiency and damp heat type xerophthalmia, and the fumigating liquid can increase local water, is beneficial to local blood vessel expansion, promotes blood circulation, and is beneficial to local nutrition supply and inflammation elimination in the using process.

The second purpose is to provide a preparation method of the fumigation liquid for the yin deficiency damp-heat type xerophthalmia, which has the advantages of simple process, convenient operation, low energy consumption, no pollution, no residual solvent, high product purity and difficult damage of effective components.

2. Technical scheme

In order to solve the above problems, the present invention adopts the following technical solutions.

A fumigation solution for yin deficiency and damp heat type xerophthalmia comprises active ingredients which are synchronously extracted from the following traditional Chinese medicinal materials in parts by weight by an SFE extraction method in 75-80 vt% ethanol per 100 parts by volume:

8-15 parts of rhizoma atractylodis

5-10 parts of phellodendron bark

5-15 parts of achyranthes root

10-20 parts of almond

5-10 parts of fructus amomi

8-15 parts of yerbadetajo herb

5-10 parts of lophatherum gracile

5-10 parts of prepared rehmannia root.

Further, the medicinal materials comprise the following raw material components:

10 portions of rhizoma atractylodis

6 portions of phellodendron

10 parts of achyranthes root

15 parts of almond

6 portions of amomum fruit

12 portions of yerbadetajo herb

Lophatherum gracile 9 parts

9 parts of prepared rehmannia root.

A preparation method of fumigation liquid for yin deficiency and damp heat type xerophthalmia is characterized by comprising the following steps:

a. rinsing the above Chinese medicinal materials with clear water, drying at 60-70 deg.C, and sterilizing.

b. B, respectively crushing the Chinese medicinal raw materials obtained in the step a by using a Chinese medicinal material crusher, and sieving the crushed Chinese medicinal raw materials by using a 20-40-mesh sieve for later use.

c. And c, putting the Chinese medicinal powder obtained in the step b into a container, fully mixing, and then putting into an extraction kettle of a supercritical device.

d. Adding a composite modifier consisting of 300ml of 75-80 vt% ethanol and 10ml of Tween 80 into the extraction kettle by a liquid adding pump in the extraction process, extracting for 2-3 hours, and collecting the extract liquor from the separation kettle I and the separation kettle II.

e. And d, mixing the extraction liquids obtained in the two separation kettles in the step d, shaking up, and packaging to obtain the mixed liquid, namely the fumigating liquid for treating yin deficiency and damp heat type xerophthalmia.

Further, the supercritical fluid used in the supercritical device in step c may be any one of carbon dioxide, nitrous oxide, sulfur hexafluoride, methane, ethane, heptane, ammonia, and the like.

Further, the supercritical fluid extraction conditions in the step c are that the extraction temperature is 15-60 ℃ and the extraction pressure is 20-50 MPa.

Further, the extraction liquid in the step d is separated under the conditions that the pressure of the separation kettle I and the pressure of the separation kettle II are 6-15 MPa and the separation temperature is 20-45 ℃.

Furthermore, in the pressure relief process in the step d, the pressure relief speed in the extraction kettle is 0.001-0.10 MPa/s.

3. Advantageous effects

Compared with the prior art, the invention has the advantages that:

the fumigation liquid in the scheme can increase local water liquid in the using process, is beneficial to local blood vessel expansion and blood circulation promotion, is beneficial to local nutrition supply and inflammation elimination, can disperse exogenous evil through hot water vapor, dredge channels and collaterals, promote qi and blood circulation, eliminate red and reduce swelling, tear and relieve itching, has good effect, is simple and easy to operate, and can be used for long-term insisting treatment on patients.

Secondly, the fumigation solution in the scheme adopts a Supercritical Fluid Extraction (SFE) technology, and has the advantages of low energy consumption, simple process flow, no environmental pollution, easy parameter control, high Extraction efficiency, good product purity and the like. Meanwhile, the method is suitable for separating and extracting volatile components and fat-soluble components which are unstable and easy to oxidize.

Thirdly, crushing the raw materials of the traditional Chinese medicines, so that the powdery medicinal materials can be more fully mixed in later-stage processing, and the extraction efficiency is higher.

And fourthly, before the traditional Chinese medicines are crushed, the traditional Chinese medicines are washed by clear water and then dried for sterilization, so that the quality of the prepared fumigation liquid can be ensured, and unnecessary side effects cannot be brought to patients.

And fifthly, in the pressure relief process, the pressure relief speed in the extraction kettle is 0.001-0.10 MPa/s, so that the traditional Chinese medicine powder cannot escape into the separation kettle along with the supercritical fluid penetrating through the plugging net, and the pipeline is polluted.

Drawings

FIG. 1 is a flow chart of the fumigant preparation of the present invention;

Detailed Description

The technical scheme in the embodiment of the invention is clearly and completely described below; obviously; the described embodiments are only some of the embodiments of the invention; but not all embodiments, are based on the embodiments of the invention; all other embodiments obtained by a person skilled in the art without making any inventive step; all fall within the scope of protection of the present invention.

Example 1:

a fumigating liquid for yin deficiency and damp heat type xerophthalmia is prepared from the following medicinal materials:

8 portions of rhizoma atractylodis

8 portions of phellodendron bark

Achyranthes and cyathula root 11 parts

13 parts of almond

7 portions of amomum fruit

13 portions of yerbadetajo herb

Lophatherum gracile 9 parts

8 parts of prepared rehmannia root.

Referring to fig. 1, a method for preparing a fumigation solution for dry eye due to yin deficiency and damp-heat includes the following steps:

a. the Chinese medicinal raw materials are dried at 60 ℃ after being rinsed with clear water and then are sterilized, so that the quality of the prepared fumigation liquid can be ensured, and unnecessary side effects can not be brought to patients.

b. B, respectively crushing the Chinese medicinal raw materials obtained in the step a by using a Chinese medicinal material crusher, and sieving the crushed Chinese medicinal raw materials by using a 30-mesh sieve for later use, so that the powdery Chinese medicinal materials can be more fully mixed, and the extraction efficiency is higher.

d. Adding a composite modifier consisting of ethanol with the concentration of 80vt percent and tween 80 with the concentration of 10ml into the extraction process by a liquid adding pump, extracting for 2 hours, and collecting extract liquor from a separation kettle I and a separation kettle II.

Further, the supercritical fluid extraction conditions in the step c are that the extraction temperature is 35 ℃ and the extraction pressure is 40 MPa.

Further, the extraction liquid in the step d is separated under the conditions that the pressure of the separation kettle I and the separation kettle II is 8MPa, and the separation temperature is 35 ℃.

Furthermore, in the pressure relief process in the step d, the pressure relief speed in the extraction kettle is slower to 0.05MPa/s, so that the traditional Chinese medicine powder cannot escape into the separation kettle along with the penetration of the supercritical fluid through the plugging net, and the pipeline is polluted.

Example 2:

10 portions of rhizoma atractylodis

7 portions of phellodendron bark

Achyranthes and cyathula root 8 parts

13 parts of almond

8 portions of amomum fruit

14 portions of yerbadetajo herb

Lophatherum gracile 8 parts

9 parts of prepared rehmannia root.

a. the Chinese medicinal raw materials are dried at 45 ℃ after being rinsed with clear water and then are sterilized, so that the quality of the prepared fumigation liquid can be ensured, and unnecessary side effects can not be brought to patients.

b. And (b) respectively crushing the Chinese medicinal raw materials obtained in the step (a) by using a Chinese medicinal material crusher and sieving the crushed Chinese medicinal raw materials by using a 35-mesh sieve for later use, so that the powdery Chinese medicinal materials can be more fully mixed, and the extraction efficiency is higher.

d. And adding a composite modifier consisting of ethanol with the concentration of 75vt percent and 10ml of Tween 80 into the extraction kettle by a liquid adding pump in the extraction process, extracting for 3 hours, and collecting extract liquor from a separation kettle I and a separation kettle II.

Further, the supercritical fluid extraction conditions in the step c are that the extraction temperature is 45 ℃ and the extraction pressure is 50 MPa.

Further, the extraction liquid in the step d is separated under the conditions that the pressure of the separation kettle I and the separation kettle II is 10MPa and the separation temperature is 40 ℃.

Furthermore, in the pressure relief process in the step d, the pressure relief speed in the extraction kettle is slower to 0.08MPa/s, so that the traditional Chinese medicine powder cannot escape into the separation kettle along with the penetration of the supercritical fluid through the net plug, and the pipeline is polluted.

The above; but are merely preferred embodiments of the invention; the scope of the invention is not limited thereto; any person skilled in the art is within the technical scope of the present disclosure; the technical scheme and the improved concept of the invention are equally replaced or changed; are intended to be covered by the scope of the present invention.

Claims

1. A fumigating liquid for xerophthalmia caused by yin deficiency and damp heat is characterized in that: the ethanol with the concentration of 75-80 vt% per 100 volume parts contains active ingredients synchronously extracted from the following traditional Chinese medicinal materials in parts by weight by an SFE extraction method:

2. the fumigating liquid for dry eye with syndrome of yin deficiency and dampness-heat according to claim 1, wherein the raw materials of the medicinal materials comprise:

3. the method for preparing fumigation solution for dry eye syndrome due to yin deficiency and dampness-heat as recited in claim 1, comprising the following steps:

4. The method for preparing fumigation solution for dry eye syndrome due to yin deficiency and dampness-heat as claimed in claim 3, wherein: the supercritical fluid used in the supercritical device in step c may be any one of carbon dioxide, nitrous oxide, sulfur hexafluoride, methane, ethane, heptane, ammonia, etc.

5. The method for preparing fumigation solution for dry eye syndrome due to yin deficiency and dampness-heat as claimed in claim 3, wherein: in the step c, the supercritical fluid extraction conditions are that the extraction temperature is 15-60 ℃ and the extraction pressure is 20-50 MPa.

6. The method for preparing fumigation solution for dry eye syndrome due to yin deficiency and dampness-heat as claimed in claim 3, wherein: and d, separating the extraction liquid in the step d under the conditions that the pressure of the separation kettle I and the pressure of the separation kettle II are 6-15 MPa and the separation temperature is 20-45 ℃.

7. The method for preparing fumigation solution for dry eye syndrome due to yin deficiency and dampness-heat as claimed in claim 3, wherein: and d, in the pressure relief process in the step d, the pressure relief speed in the extraction kettle is 0.001-0.10 MPa/s.