CN112006952A

CN112006952A - Application of rose extract extracted by using skin beautifying liquid as extraction solvent

Info

Publication number: CN112006952A
Application number: CN202010468643.4A
Authority: CN
Inventors: 黄俊永; 白秉烈; 吴杻真; 崔向兑
Original assignee: Amorepacific Corp
Current assignee: Amorepacific Corp
Priority date: 2019-05-30
Filing date: 2020-05-28
Publication date: 2020-12-01
Also published as: KR20200137432A

Abstract

The present specification discloses the use of a rose extract extracted with a skin cosmetic liquid as an extraction solvent for the preparation of a composition for moisturizing the skin. Specifically, the composition of the present invention comprises a rose extract using an effective and safe extraction solvent, thereby enabling excellent skin safety and usability, and excellent skin moisturizing effect. In addition, the composition of the present invention comprises a rose extract extracted using a skin cosmetic liquid that can be used as a cosmetic ingredient as an extraction solvent, thereby being capable of maintaining the fragrance of rose flowers excellently without additional fragrance.

Description

Application of rose extract extracted by using skin beautifying liquid as extraction solvent

Technical Field

The present disclosure relates to a skin moisturizing composition comprising rose extract extracted from skin beauty fluid as an extraction solvent as an active ingredient.

Background

Extraction (extraction) is an operation of dissolving only a specific component from a solid or liquid mixture mainly using a liquid solvent to separate the component, and is a very important separation method not only in chemical and biological experiments but also in industry. In this case, chemical reactions such as acid-base or chelate formation are used, or simply the solubility difference can be used. The case of extraction from solids is called solid-liquid extraction, the case of extraction from liquids is called liquid-liquid extraction, and there are also cases where solid-liquid extraction is called leaching.

In the extraction, the solvent extraction refers to the separation of a solid or liquid sample by dissolving one (or two or more in some cases) of the component substances with a solvent. In chemical analysis or using a mixture, there are cases where a component of a certain test material is readily soluble in a specific solvent and other components are insoluble in the solvent.

The extraction solvent refers to a liquid solvent used when a specific substance is dissolved and separated from a solid mixture or a liquid mixture, and water, alcohol, ether, petroleum ether, benzene, ethyl acetate, chloroform, mercury, and the like correspond thereto. Recently, an extraction method using a supercritical fluid (SCF) as a solvent is also frequently used.

Although the extraction thus uses only a solvent, it is also extracted from the mixture by a chemical reaction based on an acid-base reaction or chelate formation. When the solid mixture was extracted with a solvent, a Soxhlet extractor (Soxhlet extractor) was used, and when the liquid mixture was extracted, a separatory funnel was used.

Conventional cosmetic compositions containing plant extracts are used in a method of extracting a raw material by a generally used and widely used extraction method, reducing the raw material again, and putting the reduced raw material into a cosmetic composition. As the extraction method widely used in the above-mentioned fields, there is generally used a method of extracting with an organic solvent, water or a mixture thereof as a solvent, evaporating and removing the solvent from the extracted liquid phase, and then re-processing the solvent into a form usable for cosmetics to use the same for cosmetics.

However, although the use of such a general extraction method is effective in terms of cost and time, there is a possibility that only a component having solubility in a solvent among components having an original substance to be extracted is partially extracted or a part of the component is lost in an evaporation process after extraction. In addition, the organic solvent used may remain in a small amount, and an adverse reaction may occur when the extract is applied to the skin, and for this reason, the skin efficacy of the extract may be reduced, and it may be difficult to perform the function of the cosmetic composition, and there is a possibility that a problem of skin safety may occur.

Disclosure of Invention

An object of one aspect of the present invention is to provide a composition comprising a rose extract using an effective and safe extraction solvent, thereby being excellent in skin safety and usability and excellent in anti-inflammatory and moisturizing effects.

In addition, it is an object of an aspect of the present invention to provide a composition comprising a rose extract extracted using a skin cosmetic liquid that can be used as a cosmetic ingredient as an extraction solvent, thereby excellently maintaining the fragrance of rose flowers without additional fragrance.

In order to achieve the above object, one aspect of the present invention provides a skin moisturizing composition comprising a rose extract extracted from a skin cosmetic liquid as an extraction solvent as an active ingredient.

The composition of one aspect of the present invention comprises a rose extract using an effective and safe extraction solvent, and thus can be excellent in skin safety and usability, and excellent in skin moisturizing effect.

In addition, the composition of an aspect of the present invention includes a rose extract extracted using a skin cosmetic liquid that can be used as a cosmetic ingredient as an extraction solvent, thereby being capable of excellently maintaining the fragrance of rose flowers without additional fragrance.

Drawings

Fig. 1 shows the results of evaluating the skin moisturizing effect of the rose extract of test example 2 of the present invention.

Detailed Description

In the present specification, the term "skin cosmetic liquid" is a generic term for a composition having fluidity which can be applied to the skin for various cosmetic purposes, and mainly includes liquid phase or essential oil type such as soft lotion, nutritional lotion, emulsion, etc., and may include oils, solubilizers, moisturizers, water and other agents.

In the present specification, the term "extract" includes all substances obtained by extracting components of natural products regardless of the extraction method, the extraction solvent, the extracted components, or the form of the extract, and is a broad concept including all substances that can be obtained by processing or treating substances obtained by extracting components of natural products by other methods after extraction, and specifically, the processing or treatment may be additional fermentation or enzymatic treatment of the extract. Therefore, in the present specification, the term "extract" is a broad concept including a fermented product, a concentrated product, and a dried product.

In the present specification, a "glycerol derivative" is a compound similar to glycerol obtained by chemically changing a part of glycerol. Refers to a compound in which a hydrogen atom or a specific atomic group is replaced with another atom or atomic group in glycerin.

The present invention will be described in detail below.

In one aspect, the present invention may relate to a composition for moisturizing skin comprising a rose (Rosa hybrid) flower extract extracted from a skin cosmetic liquid as an extraction solvent as an effective ingredient.

In another aspect, the present invention may relate to the use of a rose extract extracted from a skin cosmetic liquid as an extraction solvent for preparing a composition for moisturizing skin.

As an example, the skin cosmetic liquid may comprise a cosmetic composition consisting of₂To C₂₀Diol of (2) and C₂To C₂₀More than one selected from the group consisting of trihydric alcohols of (a).

As other examples, the glycol may be one or more selected from the group consisting of butylene glycol, propylene glycol, and hexylene glycol. In addition, the trihydric alcohol may be one or more of glycerin and a glycerin derivative.

As other examples, the butanediol may be 1, 3-butanediol (1,3-butylene glycol), the propanediol may be 1, 3-propanediol (1, 3-propanediol), and the hexanediol may be 1, 2-hexanediol (1, 2-hexandiol).

As another example, the glycerol derivative may be ethylhexylglycerol (ethylhexylglycerol).

As yet another example, the skin cosmetic liquid may include glycerin, butylene glycol, propylene glycol, hexylene glycol, and ethylhexylglycerin.

As yet another example, the skin cosmetic liquid may further include at least one of D-glucose (D-glucose) and betaine (betain).

As an example, the content of the butylene glycol may be 10 to 25% by weight, compared to the total weight of the skin cosmetic liquid. If the amount exceeds 25% by weight, the resulting composition may not be used because of increased viscosity, inability to be filtered, etc., and if the amount is less than 10% by weight, the extraction effect may be significantly reduced. In one aspect, the butylene glycol may be present in an amount of 10 wt% or more, 12 wt% or more, 14 wt% or more, 16 wt% or more, 18 wt% or more, 20 wt% or more, 22 wt% or more, or 24 wt% or more, based on the total weight of the skin cosmetic liquid. The content may be 25 wt% or less, 22 wt% or less, 20 wt% or less, 18 wt% or less, 16 wt% or less, 14 wt% or less, or 12 wt% or less.

As other examples, the propylene glycol may be contained in an amount of 1 to 20% by weight, compared to the total weight of the skin cosmetic liquid. If the amount exceeds 20 wt%, the resulting composition may not be used because of increased viscosity, inability to be filtered, etc., and if the amount is less than 1 wt%, the extraction effect may be significantly reduced. In one aspect, the content may be 1 wt% or more, 4 wt% or more, 6 wt% or more, 8 wt% or more, 10 wt% or more, 12 wt% or more, 14 wt% or more, 16 wt% or more, or 18 wt% or more. The content may be 20 wt% or less, 18 wt% or less, 16 wt% or less, 14 wt% or less, 12 wt% or less, 10 wt% or less, 8 wt% or less, 6 wt% or less, 4 wt% or less, or 2 wt% or less.

As yet another example, the hexylene glycol may be contained in an amount of 0.1 to 5% by weight, compared to the total weight of the skin cosmetic liquid. If the amount exceeds 5 wt%, the resulting composition cannot be used because of increased viscosity, inability to be filtered, etc., whereas if the amount is less than 0.1 wt%, the extraction effect may be significantly reduced. In one aspect, the content may be 0.1 wt% or more, 0.4 wt% or more, 0.6 wt% or more, 0.8 wt% or more, 1 wt% or more, 1.2 wt% or more, 1.5 wt% or more, 2 wt% or more, 2.5 wt% or more, 3 wt% or more, 3.5 wt% or more, 4 wt% or more, or 4.5 wt% or more. The content may be 5 wt% or less, 4.5 wt% or less, 4 wt% or less, 3.5 wt% or less, 3 wt% or less, 2.5 wt% or less, 2 wt% or less, 1.5 wt% or less, 1.2 wt% or less, 1 wt% or less, 0.8 wt% or less, 0.6 wt% or less, 0.4 wt% or less, or 0.2 wt% or less.

As yet another example, the glycerin may be contained in an amount of 5 to 20% by weight, as compared to the total weight of the skin cosmetic liquid. If the amount exceeds 20 wt%, the resulting composition may not be used because of increased viscosity, inability to be filtered, etc., and if the amount is less than 5 wt%, the extraction effect may be significantly reduced. In one aspect, the content may be 5 wt% or more, 7 wt% or more, 9 wt% or more, 11 wt% or more, 13 wt% or more, 15 wt% or more, 17 wt% or more, or 19 wt% or more. The content may be 20 wt% or less, 18 wt% or less, 16 wt% or less, 14 wt% or less, 12 wt% or less, 10 wt% or less, 8 wt% or less, or 6 wt% or less.

As still another example, the content of the glycerin derivative may be 0.01 to 10% by weight, compared to the total weight of the skin cosmetic liquid. If the amount exceeds 10 wt%, the resulting composition may not be used because of increased viscosity, inability to be filtered, etc., and if the amount is less than 0.01 wt%, the extraction effect may be significantly reduced. In one aspect, the content may be 0.01 wt% or more, 0.05 wt% or more, 0.08 wt% or more, 0.1 wt% or more, 0.12 wt% or more, 0.14 wt% or more, 0.2 wt% or more, 0.4 wt% or more, 0.6 wt% or more, 0.8 wt% or more, 1 wt% or more, 4 wt% or more, 6 wt% or more, or 8 wt% or more. Further, the content may be 8 wt% or less, 6 wt% or less, 4 wt% or less, 1 wt% or less, 0.8 wt% or less, 0.6 wt% or less, 0.4 wt% or less, 0.2 wt% or less, 0.14 wt% or less, 0.12 wt% or less, 0.1 wt% or less, 0.08 wt% or less, 0.05 wt% or less, or 0.02 wt% or less.

According to an aspect of the invention, the weight ratio of glycerin to butylene glycol may be 1:0.5 to 5. In one aspect, the ratio may be 1:0.5 or more, 1:0.7 or more, 1:0.9 or more, 1:1.0 or more, 1:1.2 or more, 1:1.4 or more, 1:1.6 or more, 1:1.8 or more, 1:2 or more, 1:2.5 or more, 1:2.8 or more, 1:3 or more, 1:3.5 or more, 1:4 or more, or 1:4.5 or more. The ratio may be 1:5 or less, 1:4.5 or less, 1:4 or less, 1:3.5 or less, 1:3 or less, 1:2.5 or less, 1:2 or less, 1:1.5 or less, 1:1.2 or less, 1:1 or less, 1:0.8 or less, or 1:0.6 or less.

As an example, the weight ratio of butylene glycol to propylene glycol may be 1:0.04 to 2. In one aspect, the ratio may be 1:0.04 or more, 1:0.06 or more, 1:0.08 or more, 1:0.1 or more, 1:0.2 or more, 1:0.4 or more, 1:0.6 or more, 1:0.8 or more, 1:1 or more, 1:1.2 or more, 1:1.4 or more, 1:1.6 or more, or 1:1.8 or more. The ratio may be 1:2 or less, 1:1.8 or less, 1:1.6 or less, 1:1.4 or less, 1:1.2 or less, 1:1 or less, 1:0.8 or less, 1:0.6 or less, 1:0.4 or less, 1:0.2 or less, 1:0.1 or less, 1:0.08 or less, or 1:0.06 or less.

As other examples, the weight ratio of propylene glycol to hexylene glycol may be 1:0.005 to 5. In one aspect, the ratio can be 1:0.005 or more, 1:0.01 or more, 1:0.05 or more, 1:0.1 or more, 1:0.5 or more, 1:1 or more, 1:2 or more, 1:3 or more, or 1:4 or more. The ratio may be 1:5 or less, 1:4 or less, 1:3 or less, 1:2 or less, 1:1 or less, 1:0.5 or less, 1:0.1 or less, 1:0.05 or less, 1:0.01 or less, or 1:0.008 or less.

As other examples, the weight ratio of hexylene glycol to glycerin derivative may be 1:0.002 to 100. In one aspect, the ratio may be 1:0.002 or more, 1:0.005 or more, 1:0.01 or more, 1:0.05 or more, 1:0.1 or more, 1:0.5 or more, 1:1 or more, 1:5 or more, 1:10 or more, 1:20 or more, 1:30 or more, 1:40 or more, 1:50 or more, or 1:80 or more. The ratio may be 1:100 or less, 1:80 or less, 1:60 or less, 1:40 or less, 1:30 or less, 1:20 or less, 1:10 or less, 1:5 or less, 1:1 or less, 1:0.5 or less, 1:0.1 or less, 1:0.05 or less, 1:0.01 or less, or 1:0.005 or less.

As still another example, the content of D-glucose may be 0.1 to 10% by weight, compared to the total weight of the skin cosmetic liquid. If the content exceeds 10 wt%, the use thereof is not possible due to an increase in viscosity, inability to filter, etc., and if it is less than 0.01 wt%, the extraction effect may be significantly reduced. In one aspect, the content of D-glucose may be 0.1 wt% or more, 0.5 wt% or more, 1 wt% or more, 1.5 wt% or more, 2 wt% or more, 2.5 wt% or more, 3 wt% or more, 3.5 wt% or more, 4 wt% or more, 4.5 wt% or more, 5 wt% or more, 5.5 wt% or more, 6 wt% or more, 7 wt% or more, 8 wt% or more, or 9 wt% or more. The content may be 10 wt% or less, 9 wt% or less, 8 wt% or less, 7 wt% or less, 6 wt% or less, 5.5 wt% or less, 5 wt% or less, 4.5 wt% or less, 4 wt% or less, 3.5 wt% or less, 3 wt% or less, 2.5 wt% or less, 2 wt% or less, 1.5 wt% or less, 1 wt% or less, or 0.5 wt% or less.

As yet another example, the betaine may be contained in an amount of 0.1 to 10 wt% compared to the total weight of the skin cosmetic liquid. If the content exceeds 10 wt%, the use thereof is not possible due to an increase in viscosity, inability to filter, etc., and if it is less than 0.01 wt%, the extraction effect may be significantly reduced. In one aspect, the betaine content may be 0.1 wt% or more, 0.5 wt% or more, 1 wt% or more, 1.5 wt% or more, 2 wt% or more, 2.5 wt% or more, 3 wt% or more, 3.5 wt% or more, 4 wt% or more, 4.5 wt% or more, 5 wt% or more, 5.5 wt% or more, 6 wt% or more, 7 wt% or more, 8 wt% or more, or 9 wt% or more. The content may be 10 wt% or less, 9 wt% or less, 8 wt% or less, 7 wt% or less, 6 wt% or less, 5.5 wt% or less, 5 wt% or less, 4.5 wt% or less, 4 wt% or less, 3.5 wt% or less, 3 wt% or less, 2.5 wt% or less, 2 wt% or less, 1.5 wt% or less, 1 wt% or less, or 0.5 wt% or less.

As an example, the rose flower extract may be extracted by ultrasonic extraction, normal temperature extraction, cold soaking extraction, or reflux cooling extraction, but the extraction method is not limited thereto.

As other examples, the rose extract may be extracted at 25 to 30 ℃. For example, the rose extract may be extracted at 25 ℃ or higher, 26 ℃ or higher, 27 ℃ or higher, 28 ℃ or higher, or 29 ℃ or higher, or may be extracted at 30 ℃ or lower, 29 ℃ or lower, 28 ℃ or lower, 27 ℃ or lower, or 26 ℃ or lower.

As yet another example, the rose extract may be extracted for 150 to 210 minutes. For example, the rose extract may be extracted for 150 minutes or more, 160 minutes or more, 170 minutes or more, 180 minutes or more, 190 minutes or more, or 200 minutes or more, or may be extracted for 210 minutes or less, 200 minutes or less, 190 minutes or less, 180 minutes or less, 170 minutes or less, or 160 minutes or less.

As an example, the rose extract may be included in an amount of 1 to 100 wt% compared to the total weight of the composition for the purpose of target effect. As another example, the content of the rose extract may be 1 wt% or more, 5 wt% or more, 10 wt% or more, 15 wt% or more, 20 wt% or more, 30 wt% or more, 40 wt% or more, 50 wt% or more, 60 wt% or more, 70 wt% or more, 80 wt% or more, or 90 wt% or more, as compared to the total weight of the composition, and may be 100 wt% or less, 90 wt% or less, 80 wt% or less, 70 wt% or less, 60 wt% or less, 50 wt% or less, 40 wt% or less, 30 wt% or less, 20 wt% or less, 15 wt% or less, 10 wt% or less, or 5 wt% or less.

As an example, the composition may be a cosmetic composition.

The cosmetic composition of one aspect of the invention may contain a cosmetically or dermatologically acceptable medium or base. It is of all formulations suitable for topical application, and may be provided, for example, in the form of solutions, gels, solids, anhydrous dough products, emulsions obtained by dispersing an oily phase in an aqueous phase, suspensions, microemulsions, microcapsules, finely divided particulate spheres or dispersions of ionic (liposomes) and non-ionic vesicles, or in the form of creams, lotions, emulsions, powders, ointments, sprays or concealer sticks. These compositions may be prepared according to methods common in the art. The cosmetic composition may also be used in the form of an aerosol composition further comprising a propellant compressed into a foam (foam) form.

The cosmetic composition is not particularly limited in its formulation, and may be appropriately selected according to the purpose. For example, it can be formulated into skin lotion, skin softener, skin toner, milky lotion, milk lotion, moisturizing lotion, nourishing lotion, massage cream, nourishing cream, moisturizing cream, hand cream, foundation, essence, nourishing essence, pack, soap, cleansing foam, cleansing lotion, cleansing cream, cleansing water, powder, body lotion, body cream, body oil, body wash, body essence, and the like.

When the composition is in the form of a paste, cream or gel, animal fibers, plant fibers, wax, paraffin, starch, tragacanth, cellulose derivatives, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide can be used as a carrier component.

When the composition is prepared as a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate, polyamide powder, or the like can be used as a carrier component, and particularly, when the composition is sprayed, a propellant such as chlorofluorocarbon, propane/butane, or dimethyl ether can be added.

When the composition is prepared as a solution or emulsion, a solvent, solubilizer, emulsifier, or the like can be used as the carrier component, and for example, water, ethanol, isopropyl alcohol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1,3-butylene glycol oil, glycerol aliphatic ester, polyethylene glycol, or fatty acid ester of sorbitan is present.

When the composition is prepared as a suspension, water, a liquid diluent such as ethanol or propylene glycol, a suspension such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester or polyoxyethylene sorbitol ester, microcrystalline cellulose, aluminum metahydroxide, bentonite, agar or tragacanth, or the like can be used as a carrier component.

When the composition is formulated as a surfactant-containing face cleanser, fatty alcohol sulfate, fatty alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyl taurate, sarcosinate, fatty acid amide ether sulfate, alkylamino betaine, fatty alcohol, fatty acid glyceride, fatty acid diethanolamide, vegetable oil, lanolin derivative, ethoxylated glycerin fatty acid ester, or the like can be used as the carrier component.

In the cosmetic composition, functional additives and ingredients contained in general cosmetic compositions may be additionally included in addition to the rose extract. As the functional supplement, a component selected from the group consisting of water-soluble vitamins, fat-soluble vitamins, polymer peptides, polymer polysaccharides, sphingolipids, and seaweed extracts may be included.

In addition, in the composition, components contained in a general cosmetic composition may be combined with the functional additives as needed. Examples of the formulation components to be further contained include an oil component, a humectant, a lubricant, a surfactant, organic and inorganic pigments, organic powders, an ultraviolet absorber, a preservative, a bactericide, an antioxidant, a plant extract, a pH adjuster, alcohol, a pigment, a perfume, a blood circulation promoter, a cold feeling agent, an antiperspirant, purified water, and the like.

The present invention will be described in more detail below with reference to examples and test examples. However, the examples and test examples are only provided for understanding the contents of the present invention, and the scope of the present invention is not limited to the examples and test examples, and modifications, substitutions, and insertions, which are generally known in the art, may be performed, and are also included in the scope of the present invention.

Examples and comparative examples preparation of Rose flower extract

Rose extracts respectively extracted with the extraction solvents of examples 1 and 2 and comparative examples 1 to 5 having the compositions shown in table 1 below were prepared.

Specifically, 0.2g of Rosa damascona (Korea) domestically produced in Korea was immersed in 1kg of the extraction solvent of examples 1 and 2 and comparative examples 1 to 5, respectively, and extracted under reflux at 25 ℃ for 180 minutes, and then filtered through a 0.45 μm filter to prepare a rose extract.

[ Table 1]

Test example 1 evaluation of skin stability and extraction efficiency

The rose extracts extracted with the extraction solvents of examples 1 and 2 and comparative examples 1 to 5 were evaluated for skin stability and extraction efficiency.

Specifically, skin stability was evaluated for 32 healthy adult women with an average age of 36.36 years (people who had removed Psoriasis (Psoriasis), acne (ace), eczema (eczema), other skin diseases, etc. before the test, or pregnant and lactating women, or people who were taking contraceptives, antihistamines, etc.), and a closed patch test (co-site) was performed on each extract. Each extract was applied in an amount of 20 μ l/chamber (IQ chamber), and after 24 hours of patch removal, it was checked for 30 minutes and 24 hours. Skin reactions were evaluated according to the Frosch & Kligman, CTFA guidelines.

In addition, the extraction efficiency was evaluated by comparing the remaining amount (residual) after drying 1g of each sample at 105 ℃ for 4 hours, the more the remaining amount, the higher the extraction amount, and considering whether or not the rose fragrance was maintained (+ ++++: the highest extraction efficiency/+: the lowest extraction efficiency).

The results are shown in table 2 below.

[ Table 2]

From the results of table 2 above, it was confirmed that when examples 1 and 2, which are skin cosmetic liquids of the present invention, were used as extraction solvents, the requirements for skin safety were satisfied, and the extraction efficiency was also excellent, whereas when alcohol, ethyl acetate (ethyl acetate), n-hexane (hexane), or isopropyl alcohol (comparative examples 2 to 5), which are organic solvents widely used in general extraction methods, were used as extraction solvents, the extraction efficiency was high, but the conditions for skin safety were not satisfied. In addition, it was confirmed that when purified water (comparative example 1) was used as the extraction solvent, there was no problem in terms of skin safety, but the extraction efficiency was greatly reduced.

That is, it is understood that examples 1 and 2 of the present invention are combinations of substances usable as cosmetics, and can be used as skin cosmetic liquids (cosmetics) by themselves, and unlike organic solvents such as alcohol, ethyl acetate, n-hexane, and isopropyl alcohol, they are not irritating to the skin and can be used safely, and therefore, they are possible to be applied as cosmetics without solvent removal or additional processing. Therefore, it was confirmed that the extracted rose extract was possible to be directly applied to the skin as a cosmetic composition without additional processing, and thus examples 1 and 2 were significantly superior in terms of usability compared to comparative examples using existing solvents. In addition, in the case of using the above examples 1 and 2 as an extraction solvent, the useful components of roses can be efficiently extracted and the processing process can be minimized, so that the storage stability of the extracted components is remarkably superior to that in the case of using the conventional solvent.

[ test example 2] evaluation of skin moisturizing efficacy of Rose flower extract

The skin moisturizing effect of the rose extracts extracted using the extraction solvents of example 1 and comparative example 1 was evaluated.

Skin moisturizing efficacy was evaluated for the amount of Filaggrin (FLG) gene expression in human keratinocytes (NHEK-neo, Lonza, USA). Specifically, KBM-Gold basal medium (Lonza, USA) supplemented with growth-promoting agent (KGM-Gold Single-Quot Kit, Lonza, USA) was used at 37 deg.C with 5% CO₂Under the condition of CO₂After culturing human keratinocytes in the medium, the respective rose extract treatments were performed, and after 24 hours, intracellular RNA was isolated using Trizol (Trizol reagent, Invitrogen Carlsbad, CA, USA). The isolated RNA was quantified, and cDNA was synthesized using a Reverse transcription kit (Superscript Reverse Transcriptase (RT) II kit, Invitrogen), and then quantitatively analyzed by real-time polymerase chain reaction (RT-PCR). Use of silk fibroin expression pattern variation

Gene expression kit (gene expression assay kit) for evaluationAnd (4) price. The results of using the RPLPO gene as an internal control (internal control) gene are shown in fig. 1. In fig. 1% all are weight%.

From the results of fig. 1, it was confirmed that the rose extract of example 1 treated at the same concentration had a predominant expression level of the filaggrin gene compared to the rose extract of comparative example 1 treated at 1 wt%, and that the expression level of the filaggrin gene increased in proportion to the concentration in the case of the rose extract of example 1, and particularly, the expression level of the filaggrin gene significantly increased in the case of example 1 treated at 4 wt%.

[ PREPARATION MODE EXAMPLE 1] nutritious toning lotion

Using example 1 of one aspect of the present invention, 1 wt% of rose was extracted, and 5 wt% (compared to the total weight of the extract) of squalane was added to the extract to prepare a nutritional lotion.

[ PREPARATION EXAMPLE 2] softening toning lotion

Using example 1 of one aspect of the present invention, 5 wt% of rose flowers were extracted, and 0.5 wt% (compared to the total weight of the extract) of liquid paraffin, 5 wt% of caprylic/capric triglyceride, 1.5 wt% of polysorbate 60, 5 wt% of squalane, 4 wt% of wax were added to the extract to prepare a soft lotion.

[ formulation example 3] facial mask

Using example 1 of one aspect of the present invention, 3 wt% of rose flowers were extracted, and a mask was prepared by adding 0.3 wt% (compared to the total weight of the extract) of PEG 12 nonylphenyl ether, 0.1 wt% of allantoin, 13 wt% of polyvinyl alcohol to the extract and applying it to the surface of the mask.

Claims

1. Use of a rose extract extracted with a skin cosmetic liquid as an extraction solvent for preparing a composition for moisturizing skin.

2. The use according to claim 1, wherein,

the skin cosmetic liquid comprises: one or more selected from the group consisting of butylene glycol, propylene glycol and hexylene glycol; and one or more of glycerin and glycerin derivatives.

3. The use according to claim 2, wherein,

the butanediol is 1, 3-butanediol, the propylene glycol is 1, 3-propanediol, and the hexylene glycol is 1, 2-hexanediol.

4. The use according to claim 2, wherein,

the glycerol derivative is ethylhexyl glycerol.

5. The use according to claim 2, wherein,

the skin cosmetic liquid further comprises at least one of D-glucose and betaine.

6. The use according to claim 2, wherein,

the butylene glycol is present in an amount of 10 to 25 wt% compared to the total weight of the skin cosmetic liquid;

the propylene glycol is present in an amount of 1 to 20% by weight, compared to the total weight of the skin cosmetic liquid; or

The content of hexylene glycol is 0.1 to 5% by weight, compared to the total weight of the skin cosmetic liquid.

7. The use according to claim 2, wherein,

the content of glycerin is 5 to 20% by weight compared to the total weight of the skin cosmetic liquid; or

The content of the glycerin derivative is 0.01 to 10% by weight, compared to the total weight of the skin cosmetic liquid.

8. The use according to claim 5, wherein,

the content of D-glucose is 0.1 to 10% by weight compared to the total weight of the skin cosmetic liquid; or

The betaine is contained in an amount of 0.1 to 10 wt% compared to the total weight of the skin cosmetic liquid.

9. The use according to claim 1, wherein,

the rose extract is included in an amount of 1 to 100 wt% compared to the total weight of the composition.

10. The use according to claim 1, wherein,

the composition is a cosmetic composition.