CN112004932A - 一种CRISPR/Cas效应蛋白及系统 - Google Patents

一种CRISPR/Cas效应蛋白及系统 Download PDF

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CN112004932A
CN112004932A CN201980027152.1A CN201980027152A CN112004932A CN 112004932 A CN112004932 A CN 112004932A CN 201980027152 A CN201980027152 A CN 201980027152A CN 112004932 A CN112004932 A CN 112004932A
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sequence
protein
nucleic acid
cell
seq
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CN112004932B (zh
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赖锦盛
周英思
朱金洁
张湘博
赵海铭
宋伟彬
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China Agricultural University
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome

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Abstract

Cas效应蛋白，包含此类蛋白的融合蛋白，以及编码它们的核酸分子。用于核酸编辑(例如，基因或基因组编辑)的复合物和组合物，其包含Cas效应蛋白或融合蛋白，或编码它们的核酸分子。还涉及用于核酸编辑(例如，基因或基因组编辑)的方法，其使用包含Cas效应蛋白或融合蛋白。

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PCT国内申请，说明书已公开。

Claims (48)

  1. PCT国内申请，权利要求书已公开。
CN201980027152.1A 2018-04-20 2019-04-19 一种CRISPR/Cas效应蛋白及系统 Active CN112004932B (zh)

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CN2018103602877 2018-04-20
CN201810360287 2018-04-20
PCT/CN2019/083418 WO2019201331A1 (zh) 2018-04-20 2019-04-19 一种CRISPR/Cas效应蛋白及系统

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113373130A (zh) * 2021-05-31 2021-09-10 复旦大学 Cas12蛋白、含有Cas12蛋白的基因编辑系统及应用
WO2022247873A1 (zh) * 2021-05-27 2022-12-01 中国科学院动物研究所 工程化的Cas12i核酸酶、效应蛋白及其用途

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE202019005567U1 (de) 2018-03-14 2021-02-16 Arbor Biotechnologies, Inc. Neue CRISPR-DNA-Targeting-Enzyme und -Systeme
CN113462672A (zh) * 2018-11-15 2021-10-01 中国农业大学 CRISPR-Cas12j酶和系统
US20230193243A1 (en) * 2020-05-29 2023-06-22 Arbor Biotechnologies, Inc. Compositions comprising a cas12i2 polypeptide and uses thereof
CN114277015B (zh) * 2021-03-16 2023-12-15 山东舜丰生物科技有限公司 Crispr酶以及应用
CN114480383B (zh) * 2021-06-08 2023-06-30 山东舜丰生物科技有限公司 一种具有碱基突变的同向重复序列及其应用

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2016186946A1 (en) * 2015-05-15 2016-11-24 Pioneer Hi-Bred International, Inc. Rapid characterization of cas endonuclease systems, pam sequences and guide rna elements
WO2017143071A1 (en) * 2016-02-18 2017-08-24 The Regents Of The University Of California Methods and compositions for gene editing in stem cells
WO2017176806A1 (en) * 2015-04-03 2017-10-12 Dana-Farber Cancer Institute, Inc. Composition and methods of genome editing of b cells
CN107304435A (zh) * 2016-04-22 2017-10-31 中国科学院青岛生物能源与过程研究所 一种Cas9/RNA系统及其应用

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2017176806A1 (en) * 2015-04-03 2017-10-12 Dana-Farber Cancer Institute, Inc. Composition and methods of genome editing of b cells
WO2016186946A1 (en) * 2015-05-15 2016-11-24 Pioneer Hi-Bred International, Inc. Rapid characterization of cas endonuclease systems, pam sequences and guide rna elements
WO2017143071A1 (en) * 2016-02-18 2017-08-24 The Regents Of The University Of California Methods and compositions for gene editing in stem cells
CN107304435A (zh) * 2016-04-22 2017-10-31 中国科学院青岛生物能源与过程研究所 一种Cas9/RNA系统及其应用

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
WINSTON X YAN 等: "Functionally diverse type V CRISPR-Cas systems", 《SCIENCE》 *

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2022247873A1 (zh) * 2021-05-27 2022-12-01 中国科学院动物研究所 工程化的Cas12i核酸酶、效应蛋白及其用途
CN113373130A (zh) * 2021-05-31 2021-09-10 复旦大学 Cas12蛋白、含有Cas12蛋白的基因编辑系统及应用
CN113373130B (zh) * 2021-05-31 2023-12-22 复旦大学 Cas12蛋白、含有Cas12蛋白的基因编辑系统及应用

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WO2019201331A1 (zh) 2019-10-24

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