CN111991532A - Medicine for treating aquatic vibriosis - Google Patents
Medicine for treating aquatic vibriosis Download PDFInfo
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- CN111991532A CN111991532A CN201911292124.0A CN201911292124A CN111991532A CN 111991532 A CN111991532 A CN 111991532A CN 201911292124 A CN201911292124 A CN 201911292124A CN 111991532 A CN111991532 A CN 111991532A
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Abstract
The invention discloses a medicine for treating aquatic vibriosis, which is used in the field of aquatic vibriosis prevention and treatment and comprises the following components in parts by weight: 1-3 parts of dendrobium officinale, 7-8 parts of golden cypress, 6-11 parts of eucalyptus leaves, 5-10 parts of Chinese tallow tree, 2-10 parts of humifuse euphorbia herb, 10-20 parts of common andrographis herb, 2-5 parts of honeysuckle, 2-7 parts of wild chrysanthemum, 1-5 parts of Chinese pulsatilla root, 2-6 parts of isatis root, 3-7 parts of hibiscus flower, 10-20 parts of cactus, 5-10 parts of fructus amomi and 10-15 parts of reed rhizome. Can effectively kill pathogenic vibriosis bacteria. Can avoid the problems of antibiotic abuse and ecological environment caused by the use of a large amount of antibiotics, and is beneficial to the healthy and sustainable development of the aquaculture industry with large water production.
Description
Technical Field
The invention relates to the field of prevention and treatment of aquatic vibriosis, in particular to a medicine for treating aquatic vibriosis.
Background
The vibriosis is a common disease of seawater aquatic products, is easily damaged by the vibriosis during the culture of fish and shrimp seedlings, has the pathogeny mainly of vibrio bacteria, and has the main symptoms: diseased fish and shrimp are mainly characterized by body surface ulcer, and are rotten due to head osculum, body surface bleeding and visceral organ lesion is obvious sometimes. The disease occurs every month, the period of 7-8 months is a high incidence period, and the death rate can reach more than 80 percent when the death rate is high. The current commonly used treatment means is to mix antibacterial drugs such as norfloxacin, ciprofloxacin, chloramphenicol, gentamicin and the like into bait, the dosage of the antibacterial drugs is 1-3 percent of the bait dosage, and the antibacterial drugs are continuously fed for 5-10 days. However, the use of antibiotics in large quantities not only can lead to the abuse of antibiotics and influence the ecological environment, but also can finally influence the health of human beings, thus being not beneficial to the healthy and sustainable development of the aquaculture industry with large water production.
Disclosure of Invention
Aiming at the problems and defects in reality, the invention provides a medicine for treating vibriosis in aquatic products.
In order to solve the technical problems, the technical scheme of the invention is as follows:
the medicine for treating the vibriosis in the aquatic products is characterized by comprising the following components in parts by weight: 1-3 parts of dendrobium officinale, 7-8 parts of golden cypress, 6-11 parts of eucalyptus leaves, 5-10 parts of Chinese tallow tree, 2-10 parts of humifuse euphorbia herb, 10-20 parts of common andrographis herb, 2-5 parts of honeysuckle, 2-7 parts of wild chrysanthemum, 1-5 parts of Chinese pulsatilla root, 2-6 parts of isatis root, 3-7 parts of hibiscus flower, 10-20 parts of cactus, 5-10 parts of fructus amomi and 10-15 parts of reed rhizome.
Preferably, the traditional Chinese medicine composition comprises, by weight, 1 part of dendrobium officinale, 7 parts of golden cypress, 10 parts of eucalyptus grandiflorum leaves, 8 parts of Chinese tallow tree, 6 parts of humifuse euphorbia herb, 11 parts of common andrographis herb, 2 parts of honeysuckle, 2 parts of wild chrysanthemum, 3 parts of Chinese pulsatilla root, 2 parts of isatis root, 4 parts of hibiscus flower, 12 parts of cactus, 5 parts of fructus amomi and 13 parts of reed rhizome.
Preferably, the feed also comprises 10-15 parts of a product of composite probiotic fermented scutellaria baicalensis, wherein the components of the composite probiotic fermented scutellaria baicalensis are as follows: 3% of lactobacillus casei liquid, 4% of bacillus subtilis liquid, 15% of scutellaria baicalensis, 20% of corn powder, 30% of soybean meal powder, 5% of wheat bran, 5% of brown sugar and 18% of water; the method for fermenting the scutellaria baicalensis by the composite probiotics comprises the steps of uniformly mixing the components, fermenting at 37 ℃ for 72 hours to obtain a product of the scutellaria baicalensis fermented by the composite probiotics.
Preferably, the preparation method of the lactobacillus casei bacterial liquid comprises the following steps:
uniformly mixing 15g of peptone, 10g of yeast extract powder, 20g of glucose, tween-801 ml, 0.2g of magnesium sulfate, 2g of sodium acetate, 2g of monopotassium phosphate, 2g of sodium citrate and 0.05g of manganese sulfate, then using distilled water to fix the volume to 1L, and adding 20g of agar; sterilizing the culture medium for 20 minutes by high-pressure steam at the temperature of 121 ℃ under the pressure of 0.15MPa to obtain an MRS solid culture medium for later use;
② after the lactobacillus casei is cultured, screened and purified by MRS solid culture medium, inoculating to MRS liquid culture medium for propagation, static culturing at 37 ℃ for 24 hours to obtain lactobacillus casei liquid, adjusting the effective viable count to 1x109cfu/ml。
Preferably, the preparation method of the bacillus subtilis liquid comprises the following steps:
uniformly mixing 10g of yeast extract powder, 20g of peptone and 20g of glucose, then using distilled water to fix the volume to 1L, adding 20g of agar, and sterilizing for 20 minutes by high-pressure steam at the temperature of 121 ℃ under the pressure of 0.15MPa to obtain an YDP solid culture medium for later use;
② after bacillus subtilis is cultured, screened and purified by YDP solid culture medium, inoculating the bacillus subtilis to YDP liquid culture medium for propagation, statically culturing the bacillus subtilis at 37 ℃ for 24 hours to obtain bacillus subtilis liquid, and adjusting the effective viable bacteria number to be 1x109cfu/ml。
In conclusion, the invention has the following beneficial effects: the medicament prepared from dendrobium officinale, golden cypress, eucalyptus leaves, Chinese tallow tree, humifuse euphorbia herb, common andrographis herb, honeysuckle flower, chrysanthemum indicum, Chinese pulsatilla root, isatis root, hibiscus flower, cactus, fructus amomi, reed rhizome and a product of composite probiotic fermented scutellaria baicalensis can effectively kill pathogenic vibriosis bacteria. Can avoid the problems of antibiotic abuse and ecological environment caused by the use of a large amount of antibiotics, and is beneficial to the healthy and sustainable development of the large aquaculture industry.
Detailed Description
Here, the description of the embodiments is provided to help understanding of the present invention, but the present invention is not limited thereto. In addition, the technical features involved in the embodiments of the present invention described below may be combined with each other as long as they do not conflict with each other.
In the description of the present invention, it should be noted that the terms "upper", "lower", "inner", "outer", "front", "rear", "both ends", "one end", "the other end", and the like indicate orientations or positional relationships only for convenience in describing the present invention and simplifying the description, but do not indicate or imply that the referred device or element must have a specific orientation, be constructed in a specific orientation, and be operated, and thus, should not be construed as limiting the present invention. Furthermore, the terms "first" and "second" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance.
In the description of the present invention, it should be noted that, unless otherwise explicitly stated or limited, the terms "mounted," "disposed," "connected," and the like are to be construed broadly, and for example, "connected" may be fixedly, detachably, or integrally connected; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meaning of the above terms in the present invention can be understood in specific cases to those skilled in the art.
In one embodiment, the medicament for treating the vibriosis in the aquatic products comprises the following components in parts by weight: 1 part of dendrobium officinale, 7 parts of golden cypress, 6 parts of eucalyptus leaves, 5 parts of Chinese tallow tree, 2 parts of humifuse euphorbia herb, 10 parts of common andrographis herb, 2 parts of honeysuckle, 2 parts of wild chrysanthemum, 1 part of Chinese pulsatilla root, 2 parts of isatis root, 3 parts of hibiscus flower, 10 parts of cactus, 5 parts of fructus amomi, 10 parts of reed rhizome and 10 parts of a product of composite probiotic fermented scutellaria baicalensis.
The weight ratio of the composite probiotic fermented scutellaria baicalensis is as follows: 3% of lactobacillus casei bacterial liquid, 4% of bacillus subtilis bacterial liquid, 15% of scutellaria baicalensis, 20% of corn flour, 30% of soybean meal, 5% of wheat bran, 5% of brown sugar and 18% of water; the method for fermenting the scutellaria baicalensis by the composite probiotics comprises the steps of uniformly mixing the components, fermenting at 37 ℃ for 72 hours to obtain a product of the scutellaria baicalensis fermented by the composite probiotics.
The preparation method of the lactobacillus casei bacterial liquid comprises the following steps:
uniformly mixing 15g of peptone, 10g of yeast extract powder, 20g of glucose, tween-801 ml, 0.2g of magnesium sulfate, 2g of sodium acetate, 2g of monopotassium phosphate, 2g of sodium citrate and 0.05g of manganese sulfate, then using distilled water to fix the volume to 1L, and adding 20g of agar; sterilizing the culture medium for 20 minutes by high-pressure steam at the temperature of 121 ℃ under the pressure of 0.15MPa to obtain an MRS solid culture medium for later use;
② after the lactobacillus casei is cultured, screened and purified by MRS solid culture medium, inoculating to MRS liquid culture medium for propagation, static culturing at 37 ℃ for 24 hours to obtain lactobacillus casei liquid, adjusting the effective viable count to 1x109cfu/ml。
The preparation method of the bacillus subtilis liquid comprises the following steps:
uniformly mixing 10g of yeast extract powder, 20g of peptone and 20g of glucose, then using distilled water to fix the volume to 1L, adding 20g of agar, and sterilizing for 20 minutes by high-pressure steam at the temperature of 121 ℃ under the pressure of 0.15MPa to obtain an YDP solid culture medium for later use;
② after bacillus subtilis is cultured, screened and purified by YDP solid culture medium, inoculating the bacillus subtilis to YDP liquid culture medium for propagation, statically culturing the bacillus subtilis at 37 ℃ for 24 hours to obtain bacillus subtilis liquid, and adjusting the effective viable bacteria number to be 1x109cfu/ml。
In a second embodiment, the medicine for treating vibriosis in aquatic products comprises the following components in parts by weight: 3 parts of dendrobium officinale, 8 parts of golden cypress, 11 parts of eucalyptus leaves, 10 parts of Chinese tallow tree, 10 parts of humifuse euphorbia herb, 20 parts of common andrographis herb, 5 parts of honeysuckle, 7 parts of wild chrysanthemum, 5 parts of Chinese pulsatilla root, 6 parts of isatis root, 7 parts of hibiscus flower, 20 parts of cactus, 10 parts of fructus amomi, 15 parts of reed rhizome and 15 parts of a product of composite probiotic fermented scutellaria baicalensis.
The weight ratio of the composite probiotic fermented scutellaria baicalensis is as follows: 3% of lactobacillus casei bacterial liquid, 4% of bacillus subtilis bacterial liquid, 15% of scutellaria baicalensis, 20% of corn flour, 30% of soybean meal, 5% of wheat bran, 5% of brown sugar and 18% of water; the method for fermenting the scutellaria baicalensis by the composite probiotics comprises the steps of uniformly mixing the components, fermenting at 37 ℃ for 72 hours to obtain a product of the scutellaria baicalensis fermented by the composite probiotics.
The preparation method of the lactobacillus casei bacterial liquid comprises the following steps:
uniformly mixing 15g of peptone, 10g of yeast extract powder, 20g of glucose, tween-801 ml, 0.2g of magnesium sulfate, 2g of sodium acetate, 2g of monopotassium phosphate, 2g of sodium citrate and 0.05g of manganese sulfate, then using distilled water to fix the volume to 1L, and adding 20g of agar; sterilizing the culture medium for 20 minutes by high-pressure steam at the temperature of 121 ℃ under the pressure of 0.15MPa to obtain an MRS solid culture medium for later use;
② after the lactobacillus casei is cultured, screened and purified by MRS solid culture medium, inoculating to MRS liquid culture medium for propagation, static culturing at 37 ℃ for 24 hours to obtain lactobacillus casei liquid, adjusting the effective viable count to 1x109cfu/ml。
The preparation method of the bacillus subtilis liquid comprises the following steps:
uniformly mixing 10g of yeast extract powder, 20g of peptone and 20g of glucose, then using distilled water to fix the volume to 1L, adding 20g of agar, and sterilizing for 20 minutes by high-pressure steam at the temperature of 121 ℃ under the pressure of 0.15MPa to obtain an YDP solid culture medium for later use;
② after bacillus subtilis is cultured, screened and purified by YDP solid culture medium, inoculating the bacillus subtilis to YDP liquid culture medium for propagation, statically culturing the bacillus subtilis at 37 ℃ for 24 hours to obtain bacillus subtilis liquid, and adjusting the effective viable bacteria number to be 1x109cfu/ml。
In a third embodiment, the medicine for treating vibriosis in aquatic products comprises the following components in parts by weight: 2 parts of dendrobium officinale, 7 parts of golden cypress, 8 parts of eucalyptus leaves, 8 parts of Chinese tallow tree, 6 parts of humifuse euphorbia herb, 15 parts of common andrographis herb, 4 parts of honeysuckle, 5 parts of wild chrysanthemum, 3 parts of Chinese pulsatilla root, 4 parts of isatis root, 5 parts of hibiscus flower, 15 parts of cactus, 8 parts of fructus amomi, 13 parts of reed rhizome and 15 parts of a product of composite probiotic fermented scutellaria baicalensis.
The weight ratio of the composite probiotic fermented scutellaria baicalensis is as follows: 3% of lactobacillus casei bacterial liquid, 4% of bacillus subtilis bacterial liquid, 15% of scutellaria baicalensis, 20% of corn flour, 30% of soybean meal, 5% of wheat bran, 5% of brown sugar and 18% of water; the method for fermenting the scutellaria baicalensis by the composite probiotics comprises the steps of uniformly mixing the components, fermenting at 37 ℃ for 72 hours to obtain a product of the scutellaria baicalensis fermented by the composite probiotics.
The preparation method of the lactobacillus casei bacterial liquid comprises the following steps:
uniformly mixing 15g of peptone, 10g of yeast extract powder, 20g of glucose, tween-801 ml, 0.2g of magnesium sulfate, 2g of sodium acetate, 2g of monopotassium phosphate, 2g of sodium citrate and 0.05g of manganese sulfate, then using distilled water to fix the volume to 1L, and adding 20g of agar; sterilizing the culture medium for 20 minutes by high-pressure steam at the temperature of 121 ℃ under the pressure of 0.15MPa to obtain an MRS solid culture medium for later use;
② Lactobacillus casei processAfter MRS solid culture medium culture, screening and purification, inoculating into MRS liquid culture medium for propagation, static culturing at 37 deg.C for 24 hr to obtain lactobacillus casei bacterial liquid, adjusting effective viable count to 1x109cfu/ml。
The preparation method of the bacillus subtilis liquid comprises the following steps:
uniformly mixing 10g of yeast extract powder, 20g of peptone and 20g of glucose, then using distilled water to fix the volume to 1L, adding 20g of agar, and sterilizing for 20 minutes by high-pressure steam at the temperature of 121 ℃ under the pressure of 0.15MPa to obtain an YDP solid culture medium for later use;
② after bacillus subtilis is cultured, screened and purified by YDP solid culture medium, inoculating the bacillus subtilis to YDP liquid culture medium for propagation, statically culturing the bacillus subtilis at 37 ℃ for 24 hours to obtain bacillus subtilis liquid, and adjusting the effective viable bacteria number to be 1x109cfu/ml。
In the fourth embodiment, the medicine for treating the aquatic vibriosis comprises the following components, by weight, 1 part of dendrobium officinale, 7 parts of golden cypress, 10 parts of eucalyptus grandiflorum leaves, 8 parts of Chinese tallow tree, 6 parts of humifuse euphorbia herb, 11 parts of common andrographis herb, 2 parts of honeysuckle, 2 parts of wild chrysanthemum, 3 parts of Chinese pulsatilla root, 2 parts of isatis root, 4 parts of hibiscus flower, 12 parts of cactus, 5 parts of fructus amomi, 13 parts of reed rhizome and 15 parts of a product of composite probiotic fermented scutellaria baicalensis.
The weight ratio of the composite probiotic fermented scutellaria baicalensis is as follows: 3% of lactobacillus casei bacterial liquid, 4% of bacillus subtilis bacterial liquid, 15% of scutellaria baicalensis, 20% of corn flour, 30% of soybean meal, 5% of wheat bran, 5% of brown sugar and 18% of water; the method for fermenting the scutellaria baicalensis by the composite probiotics comprises the steps of uniformly mixing the components, fermenting at 37 ℃ for 72 hours to obtain a product of the scutellaria baicalensis fermented by the composite probiotics.
The preparation method of the lactobacillus casei bacterial liquid comprises the following steps:
uniformly mixing 15g of peptone, 10g of yeast extract powder, 20g of glucose, tween-801 ml, 0.2g of magnesium sulfate, 2g of sodium acetate, 2g of monopotassium phosphate, 2g of sodium citrate and 0.05g of manganese sulfate, then using distilled water to fix the volume to 1L, and adding 20g of agar; sterilizing the culture medium for 20 minutes by high-pressure steam at the temperature of 121 ℃ under the pressure of 0.15MPa to obtain an MRS solid culture medium for later use;
② the lactobacillus casei is cultured, screened and purified by MRS solid culture medium toThen inoculating into MRS liquid culture medium for propagation, static culturing at 37 deg.C for 24 hr to obtain lactobacillus casei bacterial liquid with effective viable count of 1x109cfu/ml。
The preparation method of the bacillus subtilis liquid comprises the following steps:
uniformly mixing 10g of yeast extract powder, 20g of peptone and 20g of glucose, then using distilled water to fix the volume to 1L, adding 20g of agar, and sterilizing for 20 minutes by high-pressure steam at the temperature of 121 ℃ under the pressure of 0.15MPa to obtain an YDP solid culture medium for later use;
② after bacillus subtilis is cultured, screened and purified by YDP solid culture medium, inoculating the bacillus subtilis to YDP liquid culture medium for propagation, statically culturing the bacillus subtilis at 37 ℃ for 24 hours to obtain bacillus subtilis liquid, and adjusting the effective viable bacteria number to be 1x109cfu/ml。
The invention is not limited to the described embodiments. It will be apparent to those skilled in the art that various changes, modifications, substitutions and alterations can be made in the embodiments without departing from the principles and spirit of the invention, and the scope of the invention is to be accorded the broadest interpretation so as to encompass all such modifications and alterations as is within the broadest scope of the claims set forth herein.
Claims (5)
1. The medicine for treating the vibriosis in the aquatic products is characterized by comprising the following components in parts by weight: 1-3 parts of dendrobium officinale, 7-8 parts of golden cypress, 6-11 parts of eucalyptus leaves, 5-10 parts of Chinese tallow tree, 2-10 parts of humifuse euphorbia herb, 10-20 parts of common andrographis herb, 2-5 parts of honeysuckle, 2-7 parts of wild chrysanthemum, 1-5 parts of Chinese pulsatilla root, 2-6 parts of isatis root, 3-7 parts of hibiscus flower, 10-20 parts of cactus, 5-10 parts of fructus amomi and 10-15 parts of reed rhizome.
2. The medicament for treating vibriosis aquaticus as claimed in claim 1, wherein: the traditional Chinese medicine composition comprises, by weight, 1 part of dendrobium officinale, 7 parts of golden cypress, 10 parts of eucalyptus leaves, 8 parts of Chinese tallow tree, 6 parts of humifuse euphorbia herb, 11 parts of common andrographis herb, 2 parts of honeysuckle, 2 parts of wild chrysanthemum, 3 parts of Chinese pulsatilla root, 2 parts of isatis root, 4 parts of hibiscus flower, 12 parts of cactus, 5 parts of fructus amomi and 13 parts of reed rhizome.
3. The medicament for treating vibriosis aquaticus according to any one of claims 1 or 2, wherein: the composite probiotic fermented scutellaria baicalensis also comprises 10-15 parts of a product of composite probiotic fermented scutellaria baicalensis, wherein the composite probiotic fermented scutellaria baicalensis comprises the following components in parts by weight: 3% of lactobacillus casei bacterial liquid, 4% of bacillus subtilis bacterial liquid, 15% of scutellaria baicalensis, 20% of corn flour, 30% of soybean meal, 5% of wheat bran, 5% of brown sugar and 18% of water; the method for fermenting the scutellaria baicalensis by the composite probiotics comprises the steps of uniformly mixing the components, fermenting at 37 ℃ for 72 hours to obtain a product of the scutellaria baicalensis fermented by the composite probiotics.
4. The medicament for treating vibriosis aquaticus as claimed in claim 3, wherein: the preparation method of the lactobacillus casei bacterial liquid comprises the following steps:
uniformly mixing 15g of peptone, 10g of yeast extract powder, 20g of glucose, tween-801 ml, 0.2g of magnesium sulfate, 2g of sodium acetate, 2g of monopotassium phosphate, 2g of sodium citrate and 0.05g of manganese sulfate, then using distilled water to fix the volume to 1L, and adding 20g of agar; sterilizing the culture medium for 20 minutes by high-pressure steam at the temperature of 121 ℃ under the pressure of 0.15MPa to obtain an MRS solid culture medium for later use;
② after the lactobacillus casei is cultured, screened and purified by MRS solid culture medium, inoculating to MRS liquid culture medium for propagation, static culturing at 37 ℃ for 24 hours to obtain lactobacillus casei liquid, adjusting the effective viable count to 1x109cfu/ml。
5. The medicament for treating vibriosis aquaticus as claimed in claim 3, wherein: the preparation method of the bacillus subtilis liquid comprises the following steps:
uniformly mixing 10g of yeast extract powder, 20g of peptone and 20g of glucose, then using distilled water to fix the volume to 1L, adding 20g of agar, and sterilizing by high-pressure steam at the temperature of 121 ℃ and under the pressure of 0.15MPa to obtain a YDP solid culture medium for later use;
② after bacillus subtilis is cultured, screened and purified by YDP solid culture medium, inoculating the bacillus subtilis to YDP liquid culture medium for propagation, statically culturing the bacillus subtilis at 37 ℃ for 24 hours to obtain bacillus subtilis liquid, and adjusting the effective viable count to be 1x109 cfu/ml。
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