CN111956702A - Green composition for preventing and treating African swine fever and application thereof - Google Patents

Green composition for preventing and treating African swine fever and application thereof Download PDF

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CN111956702A
CN111956702A CN202010672931.1A CN202010672931A CN111956702A CN 111956702 A CN111956702 A CN 111956702A CN 202010672931 A CN202010672931 A CN 202010672931A CN 111956702 A CN111956702 A CN 111956702A
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vitamin
swine fever
african swine
preventing
formula
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肖文
刘伟
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Hunan Emperor World Technology Co ltd
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Hunan Emperor World Technology Co ltd
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Abstract

The invention provides a green composition for preventing and treating African swine fever and application thereof, wherein the green composition for preventing and treating African swine fever comprises the following components in parts by weight: the first microbial formula comprises 20-25 weight percent of: bacillus licheniformis, bacillus subtilis, enterococcus faecalis and saccharomyces cerevisiae; the second traditional Chinese medicine formula is 70-75 wt%, and comprises: radix astragali, radix Codonopsis, radix Angelicae sinensis, Glycyrrhrizae radix, bupleuri radix, pericarpium Citri Tangerinae, radix Isatidis, flos Lonicerae, fructus crataegi, radix et rhizoma Rhei, and herba Epimedii; and a third nutritional formula, wherein the weight percentage is 3-6, and the third nutritional formula comprises the following components: vitamin A and vitamin D3Vitamin K3Vitamin B1Vitamin B2Vitamin B6Vitamin B12Vitamin C, nicotinamide, D-calcium pantothenate, folic acid, biotin; and fully and uniformly stirring all the standby raw materials of the microorganism formula I, the traditional Chinese medicine formula II and the nutrition formula III according to a conventional method.

Description

Green composition for preventing and treating African swine fever and application thereof
Technical Field
The invention belongs to the technical field of animal medicine, and particularly relates to a green composition for preventing and treating African swine fever and application thereof.
Background
African swine fever, ASFV for short, is an acute, virulent and hemorrhagic infectious disease only occurring in pigs caused by African swine fever virus infecting domestic pigs or wild pigs, and once the pigs are infected, the death rate is as high as 100%. China is the first county of pig raising in the world. China produces about 7 hundred million live pigs each year (before African swine fever), and the live pigs account for about 50 percent of the world. The world's first african swine fever was discovered in kenya in 1921 and spread worldwide starting in the middle of the 20 th century. In 2018, 8 and 2, the first African swine fever in China is found in Shenyang, so that the African swine fever is transferred from northeast to the original position and is diffused from the original position to the whole country, and the stock rate of the live pigs in China falls to the lowest point of nearly ten years due to the influence of the African swine fever in 2019.
Therefore, how to safely and effectively prevent and treat the African swine fever becomes a worldwide problem in the animal medical field, so that a green composition for preventing and treating the African swine fever is urgently needed to solve the worldwide problem in the animal medical field.
Disclosure of Invention
The first purpose of the invention is to provide a green composition for preventing and treating African swine fever.
A green composition for preventing and treating African swine fever and an application thereof comprise:
the first microbial formula comprises 20-25 weight percent of: bacillus licheniformis, bacillus subtilis, enterococcus faecalis and saccharomyces cerevisiae;
the second traditional Chinese medicine formula is 70-75 wt%, and comprises: radix astragali, radix Codonopsis, radix Angelicae sinensis, Glycyrrhrizae radix, bupleuri radix, pericarpium Citri Tangerinae, radix Isatidis, flos Lonicerae, fructus crataegi, radix et rhizoma Rhei, and herba Epimedii;
and a third nutritional formula, wherein the weight percentage is 3-6, and the third nutritional formula comprises the following components: vitamin A and vitamin D3Vitamin K3Vitamin B1Vitamin B2Vitamin B6Vitamin B12Vitamin C, nicotinamide, D-calcium pantothenate, folic acid, biotin;
and fully and uniformly stirring all the standby raw materials of the microorganism formula I, the traditional Chinese medicine formula II and the nutrition formula III according to a conventional method.
Preferably, the green composition for preventing and treating African swine fever comprises:
the first microbial formula comprises 53g of bacillus licheniformis, 43g of bacillus subtilis, 33g of enterococcus faecalis and 111g of saccharomyces cerevisiae,
the preparation method of the microbial formula I comprises the following steps of fully and uniformly stirring the 4 raw materials according to a conventional method, wherein the total weight is 240g for later use;
700g of astragalus membranaceus, 820g of codonopsis pilosula, 210g of angelica sinensis, 460g of liquorice, 290g of radix bupleuri, 140g of pericarpium citri reticulatae, 300g of radix isatidis, 400g of honeysuckle, 200g of hawthorn, 600g of rheum officinale and 200g of herba epimedii, and the preparation method of the second traditional Chinese medicine formula comprises the following steps of preparing the 11 raw materials into 6: 1, and then fully stirring the mixture evenly according to a conventional method to obtain 720g (crude drug 4320g ÷ 6 ═ 720g) for later use;
nutritional formula III, vitamin A6.15 g and vitamin D31.8g, vitamin K32.2gVitamin B11g, vitamin B20.6g, vitamin B60.575g and vitamin B120.009g, vitamin C13.8g, nicotinamide 3g, D-calcium pantothenate 2.5g, folic acid 0.2g, biotin 8.166g, the preparation method of the third nutrition formulation is that the 12 raw materials are fully and evenly stirred according to the conventional method, and the total amount is 40g for standby;
fully and uniformly stirring all the standby raw materials of the microorganism formula I, the traditional Chinese medicine formula II and the nutrition formula III according to a conventional method, wherein the total amount of the raw materials is 1000 g.
Preferably, the raw materials in the first formula of the microorganism are all 1g containing 1000 hundred million units of colonies.
The African swine fever virus mainly attacks five physiological systems of pigs, namely the digestive system, the respiratory system, the blood circulation system, the excretory system and the immune system.
1) A digestive system: african swine fever viruses enter the digestive system of pigs through feed, drinking water and the like. Here, physiological changes are summarized in terms of pathological changes that are more typical in clinical manifestations.
1.1) bleeding due to gastric erosion: the stomach has four functions, one is to store food, the other is to digest mechanically, the third is to digest chemically, and the fourth is to empty. When the stomach is invaded by the African swine fever virus, the normal operation of the whole digestive system is seriously influenced, and the African swine fever virus also enters the blood circulation system through the erosion part of the stomach.
1.2) dense bleeding points on the outer membrane of the small intestine or dense bleeding points on the inner membrane of the small intestine: only a small portion of food is digested and absorbed in the stomach, and about 98% is digested and absorbed in the small intestine. When the small intestine is invaded by the African swine fever virus, not only the digestion of food and the absorption of nutrition by the small intestine are seriously influenced, but also the African swine fever virus enters the blood circulation system through the small intestine erosion part.
1.3) the main function of the large intestine is to absorb water, most of which is absorbed in the large intestine. When the large intestine is invaded by the African swine fever virus, the large intestine can absorb too much or too little water to cause constipation or diarrhea, and the African swine fever virus also enters the blood circulation system through the large intestine erosion part.
1.4) the most prominent function of the liver is the detoxification function. Toxic substances are absorbed by small intestine and enter liver, and are detoxified by liver to become nontoxic or less toxic substances and then enter blood circulation system. When the detoxifying function of the liver is completely lost, the African swine fever virus enters a blood circulation system through a small intestine 'one-way green light', and invades various tissues and organs of a body through the blood circulation, so that the pig can die in about 50 hours.
2) A respiratory system: african swine fever viruses enter the respiratory system of pigs through dust, rain fog, spray, etc. suspended in the air. Here, physiological changes are summarized in terms of pathological changes that are more typical in clinical manifestations.
2.1) tracheal annular congestion or exudation: the trachea has the function of protecting the bronchus and the lung. When the trachea is invaded by African swine fever virus, a second defense line is opened for the lung invaded by the African swine fever virus.
2.2) pulmonary swelling and congestion: the lungs are responsible for the transport of oxygen and the elimination of carbon dioxide to the organs throughout the body, and are the "hub" of the respiratory system. The upper respiratory tract (nose, pharynx and larynx) forms a first defense line for protecting the lung, the lower respiratory tract (trachea, bronchi and terminal bronchiole) forms a second defense line for protecting the lung, alveoli of the lung are bottom lines which cannot be invaded, and when the bottom lines are invaded, pneumonia, hypoxia and systemic infection are inevitable to occur; when the lungs stop working, it represents the cessation of breathing and the end of life.
3) A blood circulation system: when the digestive system and the respiratory system of the pig are invaded by the African swine fever virus, the African swine fever virus enters a blood circulation system through the digestive system and the respiratory system to destroy the immune function of blood and invade various tissues and organs of a body through the blood circulation. Here, physiological changes are summarized in terms of pathological changes that are more typical in clinical manifestations.
3.1) the pig ear tip is purple, and the skin of the head and the abdomen has bleeding spots or bleeding points: these skin lesions clinically represent three points: the first is that the blood cells in the blood are damaged by African swine fever virus, and the protective function of the blood on the body is lost; second, it represents a severe decrease in platelets that has led to the rupture of the subcutaneous capillary wall; and third, the loss of the excretory function of the skin.
3.2) hemorrhagic lesions of various tissues and organs: the African swine fever virus not only destroys the protective function of blood cells to the organism and the immunologic function of blood plasma to the organism, but also damages each tissue and organ through blood circulation, thereby causing hemorrhagic lesions of each tissue and organ.
3.3) the chest cavity and the abdominal cavity are filled with red effusion: the African swine fever virus destroys the normal blood circulation and causes the chest cavity and the abdominal cavity to be filled with red effusion.
3.4) yellow fat in the heart, congestion in heart failure, particularly dense bleeding spots in the auricle: the heart is the motile organ for blood circulation. Heart failure with congestion, representing an impaired blood circulation; once the heart ceases to function, it represents the cessation of blood circulation and the end of life.
4) An excretion system: when swine is invaded by african swine fever virus in the digestive system, respiratory system, and blood circulation system, the excretion burden of the excretory organs such as lungs, skin, digestive tract, and kidneys, particularly, the excretion burden of the kidneys is increased, and thus, each excretory organ is damaged. Here, physiological changes are summarized in terms of pathological changes that are more typical in clinical manifestations.
4.1) enlargement of the kidney, enlargement of the renal papilla, congestion: the kidney is vertically divided from abdominal aorta, is the most important excretory organ in pig body, excretes most metabolites in body and foreign matters, drugs, toxins and the like in blood in the form of urine generation and excretion, and not only has large excretion amount, but also has many varieties. When both kidneys were completely necrotic, the representation of the pig was undoubtedly.
4.2) the bladder has dense bleeding spots: urine production is accomplished by the coordinated activities of the nephron and the collecting duct. The bladder is the organ that stores urine. Bladder bleeding not only affects normal urination, but also indicates that the kidney has been severely affected by African swine fever virus.
5) The immune system: the African swine fever virus firstly attacks immune organs of a digestive system and a respiratory system and then attacks various immune organs of a body through blood circulation, so that the immune function of the pig is gradually weakened until the immune function is completely lost. Here, physiological changes are summarized in terms of pathological changes that are more typical in clinical manifestations.
5.1) spleen necrosis and enlargement 3-5 times: the spleen is the biggest immune organ in the pig body, has four functions of immunity, hematopoiesis, blood filtration and blood storage, and is a defending army of pig immunity. When the spleen dies, the immune function representing the pig falls down first.
5.2) swelling and congestion of lymph nodes: the 300-600 lymph nodes in the pig are distributed on the route of the lymph circulation of the organism, and are the 'local troops' of pig immunity. When the lymph nodes are swollen and congested, the swine represents that the immune function of the swine is 'full-force covering and extinguishing'.
In summary, since the African swine fever virus mainly attacks five physiological systems of pigs, such as the digestive system, the respiratory system, the blood circulation system, the excretory system, the immune system and the like, the invention specifically and purposefully protects the five physiological systems from being attacked by the African swine fever virus, thereby achieving the final aim of successfully preventing and treating the African swine fever.
The technical principle of the invention is realized by the combination of three technical fields of micro-ecological technology, traditional Chinese medicine technology, nutrition technology and the like, and the respective action principles, components and component functions of the three technical fields are introduced:
1) the technical field of microorganisms:
1.1) action principle: by pertinently supplementing the variety and the number of beneficial bacteria in the stomach and intestine, the disease resistance of the stomach and intestine is enhanced, the mass propagation of the beneficial bacteria in the stomach and intestine and the regeneration and self-repair of gastrointestinal mucosa cells are promoted, and the mucosa barrier with super-strong immunity is reconstructed, so that the aim of preventing the stomach and intestine from being invaded by African swine fever viruses is fulfilled.
This is the first line of defense against african swine fever.
Under the action of the technical field, even if the pigs eat feed, drinking water and the like polluted by the African swine fever virus, the African swine fever virus is difficult to penetrate through gastrointestinal mucosa barriers to enter capillaries and is discharged out of the body along with excrement; secondly, under the action of the technical field, harmful substances absorbed by the small intestine are obviously reduced, so that the detoxification burden of the liver is relieved, and the liver is not easily attacked by the African swine fever virus; thirdly, under the action of the technical field, the nutrition of the feed is fully absorbed by the small intestine, thereby not only increasing the detoxication nutrition of the liver, but also increasing the nutrition required by each tissue and organ.
1.2) composition components: bacillus subtilis, bacillus licheniformis, enterococcus faecalis and saccharomyces cerevisiae.
1.3) introduction of main functions of the components:
1.3.1) Bacillus subtilis: can reduce the number of harmful bacteria such as escherichia coli, clostridium perfringens, salmonella and the like in the intestinal tract, and increase beneficial bacteria in the organism.
1.3.2) Bacillus licheniformis: the product can rapidly colonize and reproduce after entering intestine and stomach, consume oxygen, form a low oxygen environment, and provide beneficial conditions for growth and reproduction of beneficial microorganisms such as Bacillus bifidus and lactobacillus in intestine, thereby inhibiting growth of harmful bacteria.
1.3.3) enterococcus faecalis: can soften the fiber in the feed, improve the conversion rate of the feed and simultaneously can produce various antibacterial substances.
1.3.4) Saccharomyces cerevisiae: promoting digestion and absorption of intestinal tract to feed, reducing foul smell, and enhancing immunity and disease resistance.
2) The technical field of traditional Chinese medicine is as follows:
2.1) action principle: the health of the respiratory system and the blood circulation system of the pig is protected by the unique mutual synergistic effect of the traditional Chinese medicines of clearing heat and detoxicating, strengthening body resistance and eliminating dampness, strengthening middle warmer and replenishing qi, activating blood circulation to dissipate blood stasis and softening hardness and eliminating tumor. Under the mutual synergistic effect that the two physiological systems are always in good working states, the capability of phagocytizing pathogens by macrophages is enhanced, and an ultrastrong immune mechanism is reconstructed, so that the aim of preventing the two physiological systems from being invaded by African swine fever viruses is fulfilled.
This is the second line of defense against african swine fever.
In order to more clearly illustrate the operation principle of the technical field on the respiratory system and the blood circulation system, the technical field is specifically illustrated.
2.1.1) acting on the respiratory system: under the action of the technical field, once foreign matters such as dust, water mist, spray and the like which are suspended in the air and polluted by the African swine fever virus enter the pig nose, the foreign matters can immediately stimulate the upper respiratory tract (nose, pharynx and throat) to generate defense response and are quickly discharged out of the body; secondly, under the action of the technical field, once foreign matters with the African swine fever virus enter the trachea, the foreign matters can stimulate the lower respiratory tract (trachea, bronchus and small bronchus) to generate defense response immediately, and the foreign matters are sent to the upper respiratory tract and are discharged out of the body through the upper respiratory tract; thirdly, under the action of the technical field, even if foreign matters containing the African swine fever virus enter the lung, macrophages in the lung are stimulated to generate defense and phagocytic response immediately, and the foreign matters are sent to a lower respiratory tract and then discharged out of the body through an upper respiratory tract, so that the foreign matters containing the African swine fever virus are prevented from entering the lung circulation and the blood circulation system through a respiratory system.
2.1.2) acts on the blood circulation system, under the action of the technical field, even if the African swine fever virus enters the blood circulation system through the digestive system, the respiratory system or the mosquito bite and the like, the African swine fever virus immediately stimulates the immune globulin in the blood to generate superstrong immunity, stimulates the platelet to generate superstrong protection function and stimulates the leukocyte to generate superstrong phagocytosis function, thereby protecting the blood from the invasion of the African swine fever virus; secondly, under the action of the technical field, the blood is healthy, the blood pressure is normal, and various hematopoietic organs work well, so that the heart of a power organ with blood circulation is protected from being invaded by African swine fever virus; thirdly, under the action of the technical field, the heart is always in a benign working state, power is provided to promote the circulation and flow of blood, various cells of the organism are continuously conveyed with living substances, including nutrient substances and oxygen, cell metabolite carbon dioxide is also taken away, and meanwhile, a plurality of hormones, antigens, immune synthetic substances and other information reach target organs of the organism in time through the blood circulation, so that the capability of the whole organism to resist African swine fever viruses is improved.
2.2) composition components: astragalus root, pilose asiabell root, Chinese angelica root, licorice root, bupleurum root, tangerine peel, isatis root, lonicera flower, rhubarb, haw and epimedium herb.
2.3) introduction of main functions of the components:
2.3.1) radix astragali: invigorating qi, invigorating yang, consolidating superficial resistance, arresting sweating, replenishing water, relieving swelling, resisting toxic materials, expelling pus, healing sore, and promoting granulation. It can be used for treating deficiency of lung and spleen qi, decline of middle-warmer energy, spontaneous perspiration due to exterior deficiency, edema due to qi deficiency, sore and carbuncle, and intractable ulcer.
2.3.2) radix Codonopsis: tonify middle-jiao and Qi, invigorate spleen and benefit lung. Can be used for treating weakness of spleen and stomach, anorexia, diarrhea, cough with asthma due to lung deficiency, asthenia, and qi deficiency with prolapse.
2.3.3) Angelica sinensis: tonify blood, nourish blood, activate blood and alleviate pain, moisten dryness and relax bowels. It can be used for treating blood deficiency, fatigue, blood stasis, pain, traumatic injury, carbuncle, swelling, herpetic infection, constipation, and fetal diseases.
2.3.4) licorice: invigorating spleen and replenishing qi, eliminating phlegm and relieving cough, regulating the middle warmer and relieving urgency, removing toxic substance, harmonizing the effects of the above drugs, and relieving drug toxicity and toxicity. It can be used for treating weakness of spleen and stomach, asthenia, cough, asthma, sore throat, poisoning, and pyocutaneous disease.
2.3.5) radix bupleuri: sending out the interior and exterior, lifting yang-qi, soothing the liver and relieving depression. It can be used for treating common cold, fever, alternating cold and heat, spleen deficiency, chronic diarrhea, metroptosis, and proctoptosis.
2.3.6) dried orange peel: regulate qi to invigorate spleen, dry dampness and resolve phlegm. Mainly treats anorexia, abdominal pain, abdominal distension, diarrhea, phlegm-damp cough.
2.3.7) radix isatidis: clear heat and remove toxicity, cool blood and relieve sore throat. Can be used for treating wind-heat type common cold, sore throat, aphtha, sore yellow swelling, and toxic materials.
2.3.8) honeysuckle flower: clearing away heat and toxic material, dispelling wind and dissipating heat. Can be used for treating epidemic febrile disease, fever, wind-heat type common cold, cough due to lung heat, sore throat, heat toxin, dysentery with bloody stool, breast swelling and pain, carbuncle, and skin sore.
2.3.9) rhubarb: purge heat and unblock intestines, cool blood and remove toxicity, break accumulation and remove blood stasis. Can be used for treating constipation due to excessive heat, skin ulcer, furunculosis, conjunctival congestion, swelling and pain, burn, scald, and traumatic injury. Rotten skin, enteritis.
2.3.10) hawthorn fruit: dispel qi and resolve accumulation, move qi and dissipate blood stasis. It can be used for treating abdominal distention due to overeating, dyspepsia, and puerperal lochiorrhea.
2.3.11) herba Epimedii: tonify kidney yang, strengthen tendons and bones, dispel wind-damp. Can be used for treating sexual impotence, spermatorrhea, asthenia of waist and hip, rheumatalgia, numbness, spasm, and hypertension.
3) The technical field of nutrition:
3.1) action principle: the nutrition is the basis of all life activities of pig such as survival, growth, reproduction, milk production, immunity and the like, and the nutrition cannot be separated in the whole life process. Vitamin nutrition cannot be synthesized in pigs and must be provided by the feed, or its precursors. Because the required amount of each vitamin of the pigs is different, the consumption of each vitamin can cause the metabolic disturbance of the body, generate a series of deficiency symptoms, influence the health and the production performance of the pigs and can cause the death of the pigs in serious cases; the nutrition can also cause metabolism and production disorder and even toxic death (for example, the excess vitamin can cause dyspepsia and diarrhea, and the excess fat-soluble vitamin can cause metabolism, production disorder and even toxic death), so the technical field of nutrition scientifically and comprehensively supplements the vitamin nutrition of the swine for resisting African swine fever virus by combining years of clinical experience based on the theory of nutrition.
This is the third line of defense against African swine fever!
Under the action of the technical field, the nutritional requirements of three functions of liver digestion, metabolism and detoxification are met; the nutritional requirements of the hematopoietic function of the bone marrow are met; meets the nutritional requirement of the kidney for producing the erythropoietin. Because the nutritional requirements of the liver, the bone marrow, the kidney and other organs are met, the capability of resisting the African swine fever virus of pigs is improved.
3.2) composition components: vitamin A and vitamin D3Vitamin K3Vitamin B1Vitamin B2Vitamin B6Vitamin B12Vitamin C, nicotinamide, D-calcium pantothenate, folic acid and biotin.
3.3) introduction of main functions of the components:
3.3.1) vitamin A: the substances necessary for maintaining epithelial tissue health can reduce the occurrence of pig common cold, pneumonia, nephritis and cystitis.
3.3.2) vitamin D3: vitamin D3Is necessary for normal calcification of bones.
3.3.3) vitamin K3: vitamin K3Belongs to the procoagulant drugs. Can be used for treating vitamin K3Hemorrhagic disease caused by deficiency.
3.3.4) vitamin B1: the main function is to participate in carbohydrate metabolism. Pig is deficient in vitamin B1Manifested by appetite and weight loss, slow pulse, low body temperature, neurological symptoms, myocardial edema and enlarged heart.
3.3.5) vitamin B2: lack of reserve vitamin B in animal body2The ability of the cell to perform. Lack of control in pigs often manifests itself as bending of the legs, stiffness, pachynsis, rashes, spillage on the back and sides of the skin, etc.
3.3.6) vitamin B6: the main functions are linked to the enzyme system of protein metabolism, and also participate in carbohydrate and fat metabolism, involving more than 50 enzymes in the body. Pig is deficient in vitamin B6Poor appetite and slow growth are observed.
3.3.7) vitamin B12: vitamin B12In vivo, it is mainly involved in various metabolic activities in the form of two coenzymes, dideoxyadenosine cobalamin and methylcobalamin. Lack of vitamin B12The most obvious symptom of pigs is impaired growth, which is then manifested as uncoordinated and unstable gait; pig breeding can also be affected.
3.3.8) vitamin C: the main function is to participate in collagen synthesis. Under stress conditions such as high temperature, transportation, disease, etc., the demand for vitamin C in animals increases.
3.3.9) nicotinamide: nicotinamide is involved in the metabolism of carbohydrates, lipids and proteins primarily through NAD and NADP, and plays an important role in inter alia reactions that supply energy for metabolism in vivo. Nicotinamide deficiency, pigs manifest as heaviness of the head, diarrhea, vomiting, dermatitis, and anemia of normal red blood cells.
3.3.10) D-calcium pantothenate: the lack of calcium D-pantothenate in pigs is manifested by increased skin and dandruff, capillary sensation, brown secretion around the eyes, gastrointestinal disorders, and slow growth.
3.3.11) folic acid: folate deficiency causes the growth of erythrocytes to stay in the megaloblastic stage, eventually leading to megaloblastic anemia; it also affects the formation of leukocytes in the blood, resulting in thrombocytopenia and leukopenia. Folate is essential for maintaining the normal function of the immune system.
3.3.12) biotin: many animals are caused by biotin deficiency by feeding raw egg white, biotin-free feed or by adding sulfa drugs (which inhibit intestinal microbial synthesis). The symptoms of the deficiency are generally manifested as poor growth.
The invention has the common action principle in three technical fields:
the health of the pig digestive system is protected through the technical field of microorganisms, and a first defense line for resisting African swine fever is formed; the health of a pig respiratory system and a blood circulation system is protected through the technical field of traditional Chinese medicines, and a second defense line for resisting African swine fever is formed; meets the nutritional requirements of three major systems of pigs through the technical field of nutrition, and forms a third defense line for resisting African swine fever. The three defense lines act with each other and supplement each other to form a combined defense line for resisting African swine fever. Under the action of the combined defense line, the excretion system of the pig is protected from being invaded by the African swine fever virus, the immune system of the pig is protected from being invaded by the African swine fever virus, and the aim of successfully preventing and treating the African swine fever is finally achieved.
The invention includes but is not limited to one or more of the technical fields of the microorganism, the traditional Chinese medicine and the nutrition, and all technical schemes using the same action principle and composition as the technical fields or component replacement without influencing actual effect within the technical scope of the technical personnel in the field belong to the protection scope of the invention. For example, the research and development of products by using the action principle in the technical field of traditional Chinese medicines, the product publicity by using the action effect of the first microbial formula and the like are all infringements of the invention.
In order to more clearly illustrate the action principle of joint defense on the excretory system and the immune system, the specific description is provided herein.
1) Acting on the drainage system: under the action of a joint defense line, the pig excretion system is always in a benign working state and mainly shows the following four aspects: firstly, the intestinal tract smoothly excretes hemoglobin metabolites, calcium, magnesium, iron and the like from blood, thereby relieving the excretion burden of the kidney; second, foreign substances, drugs, toxins, etc. in the blood are significantly reduced, thereby significantly reducing the burden of excretion of the kidney and the entire urinary system; thirdly, the lungs excrete carbon dioxide and water smoothly; fourth, the skin smoothly drains metabolites such as water, sodium chloride, and urea.
2) Acting on the immune system: under the action of the combined defense line, the nonspecific active immune function of the immune system is improved under the benign working state of the excretion system of the pig. It mainly appears in the following three aspects: firstly, the invading African swine fever virus can be quickly identified and eliminated; secondly, tumor cells, senescent cells, dead cells or other harmful components which are mutated in vivo can be rapidly identified and eliminated; thirdly, immune cells and damaged organs and tissues can be rapidly repaired to restore normal functions.
The second purpose of the invention is to provide the application of the green composition for preventing and treating African swine fever.
The green composition for preventing and treating African swine fever is suitable for normal prevention, emergency prevention and treatment of African swine fever.
1000g of the green composition for preventing and treating African swine fever is mixed with 1000 jin of materials or 2000 jin of drinking water and is continuously used until marketing.
1000g of the green composition for preventing and treating African swine fever is mixed with 500 jin of materials or 1000 jin of drinking water for 7 days continuously, and the mixture is continuously used according to the normal prevention dosage after 7 days until marketing.
The green composition for preventing and treating African swine fever is fed by 5g of piglets, 15g of middle pigs and 25g of big pigs twice a day in a daily amount, the feeding is continuously carried out for 7 days, half feeding is carried out for 7 days after 7 days, the feeding is carried out for 7 days according to an emergency prevention amount after half feeding is carried out for 7 days, and the feeding is carried out for 7 days according to the emergency prevention amount and then is continuously used for marketing according to a normal prevention amount.
Compared with the prior art, the invention has the beneficial effects that:
1. so far, no vaccine for safely and effectively preventing and treating African swine fever exists globally, so that the vaccine is a 'vaccine' without the vaccine, even if the vaccine comes into the market in the future, the vaccine for generating passive immunity also has the defects, and the vaccine is reused for immunization on the basis of the invention for generating active immunity, so that the defects of the vaccine can be overcome, and a more comprehensive and more definite prevention and treatment effect can be realized.
2. The invention not only can prevent and treat African swine fever, but also can simultaneously solve eleven major problems commonly existing in the breeding industry at present, which is the most existing in the prior art.
3. The invention can not only prevent and treat diseases, but also promote the growth of animals (the growth speed is improved by about 10%), which is the only thing in the prior art.
4. The raw materials related by the invention do not contain any antibiotic, banned components and animal-derived components, thereby realizing the aims of green, safety, health and high efficiency and providing a reliable technical support for accelerating the development of nonreactive breeding and green food in China.
Drawings
FIG. 1 is a schematic diagram of a green composition for preventing and treating African swine fever and an application technical scheme thereof.
Detailed Description
The present invention will be further described with reference to the accompanying drawings so as to facilitate the understanding of the present invention by those skilled in the art.
It should be noted that all the directional indicators (such as up, down, left, right, front, and rear … …) in the embodiment of the present invention are only used to explain the relative position relationship between the components, the movement situation, etc. in a specific posture (as shown in the drawing), and if the specific posture is changed, the directional indicator is changed accordingly.
In addition, the descriptions related to "first", "second", etc. in the present invention are only for descriptive purposes and are not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include at least one such feature. In the description of the present invention, "a plurality" means at least two, e.g., two, three, etc., unless specifically limited otherwise.
The preparation method of the invention is a conventional method.
The materials, reagents and the like used in the preparation method of the invention can be obtained from commercial sources.
As shown in figure 1, the formula and the preparation method of the green composition for preventing and treating African swine fever are as follows:
the formula and the preparation method thereof (hereinafter referred to as formula one) in the technical field of microorganism are as follows:
the formula is as follows: 53g of bacillus licheniformis, 43g of bacillus subtilis, 33g of enterococcus faecalis and 111g of saccharomyces cerevisiae (the raw materials in the formula are all 1g of colony containing 1000 hundred million units).
The preparation method comprises the following steps: the 4 raw materials are fully and uniformly stirred according to a conventional method, and the total weight is 240g for standby.
The formula and the preparation method thereof (the formula II is abbreviated as follows) in the technical field of traditional Chinese medicines:
the formula is as follows: 700g of astragalus, 820g of codonopsis pilosula, 210g of angelica, 460g of liquorice, 290g of radix bupleuri, 140g of dried orange peel, 300g of isatis root, 400g of honeysuckle, 200g of hawthorn, 600g of rhubarb and 200g of epimedium herb.
The preparation method comprises the following steps: preparing the above 11 raw materials into 6: 1, and then fully stirring the mixture evenly according to a conventional method to obtain a total of 720g (crude drug 4320g ÷ 6 ═ 720g) for later use.
The formula and the preparation method thereof (the formula is hereinafter referred to as the formula III) in the technical field of nutrition:
the formula is as follows: vitamin A6.15 g and vitamin D31.8g, vitamin K32.2g, vitamin B11g, vitamin B20.6g, vitamin B60.575g and vitamin B120.009g, vitamin C13.8g, nicotinamide 3g, D-calcium pantothenate 2.5g, folic acid 0.2g, and biological agent8.166g of biotin.
The preparation method comprises the following steps: the 12 raw materials are fully and uniformly stirred according to a conventional method, and the total weight is 40g for standby.
The raw materials for standby in the formula I, the formula II and the formula III are fully and uniformly stirred by a conventional method to be 1000g in total, and the formula and the preparation of the technology are satisfactorily finished.
The dosage and usage are different according to the different severity of the swine fever epidemic situation.
1. And (3) normal prevention: 1000g of the product is mixed with 1000 jin of material or 2000 jin of drinking water, and the mixture is continuously used until marketing.
2. Emergency prevention (other swine farms within 3 km had an infection of african swine fever): 1000g of the product is stirred for 500 jin or drunk for 1000 jin and is continuously used for 7 days, and after 7 days, the normal prevention dosage is changed to be continuously used until the product is put to market.
3. Treatment: one pig, one day, 5g of piglet; 15g of middle pigs; feeding 25g of big pigs twice a day, continuously feeding for 7 days, halving the feeding for 7 days after 7 days, feeding for 7 days according to the emergency prevention amount after 7 days, and continuously feeding for 7 days according to the emergency prevention amount until the big pigs are discharged.
Meanwhile, the following cautions should be followed when the product is put into practical use:
1. the pig farm is recommended to carry out 'closed prevention and control, gate-keeping disinfection and prevention and control by the invention'. The closed prevention and control means that each fence house is totally closed, and meanwhile, a water curtain is installed to control the temperature and an exhaust fan is installed to ventilate; the door-keeping disinfection means that any materials, personnel and the like entering a big door of a pig farm and a small door of a stall house can enter the pig farm only after strict disinfection; the prevention and treatment of the present invention means that the prevention and treatment are carried out strictly by the use amount and the precautions of the present invention.
2. Before treatment, pigs with infection symptoms (do not eat) are eliminated, then pigs which do not show the infection symptoms (do not eat) are treated, and during the treatment period, the pigs with the infection symptoms (do not eat) are immediately broken and subjected to harmless 'tooth extraction', namely, the pigs are eliminated at one end, and the good treatment can be firmly eliminated, but the pigs are kept in a good mood. Success cases show that following this implementation, pigs with symptoms of infection (no food) are generally reduced substantially after 7 days.
3. During the treatment period, please stop using all antibiotics. Clinical cases show that pigs die faster and more frequently in a pig farm where antibiotics are used during treatment.
4. During the prevention period, please strictly clean and disinfect the inside and outside of the pig farm and the pigsty for 1 time a day; during the treatment period, the inside and outside of the pig farm and the pigsty are strictly cleaned and disinfected, and the concentration of the disinfectant is increased for disinfection, 2 times a day.
The results of clinical comparative tests are as follows:
in order to verify the effective results of the invention, the following comparative tests were carried out under the supervision of the relevant governing departments, and the test methods and results were as follows:
example 1: control test for prevention of African swine fever.
Overview of pig farm: in 2019, 6 and 9 days, 16 pig farms are arranged in 10 kilometers of a certain county, the 16 pig farms are randomly divided into 2 groups, each group comprises 8 pig farms, the pig farms prevented by the method are used as a test group, the pig farms prevented by the method without the method are used as a control group, 180 days (6 and 9 months to 12 and 8 months in 2019) are observed, and the results are recorded as follows:
Figure RE-GDA0002727703730000111
example 2: pig farm trials to treat pigs in close contact with African Swine fever but who have not yet exhibited symptoms of infection (no food).
Overview of pig farm: in 2019, 4 and 8 days, 50 replacement gilts are self-bred and autotrophic in a certain sow farm in stock, and each replacement gilt weighs about 140 jin. In the same month, 150 middle pigs were introduced from other places and were arranged to be fed in a pen next to the self-bred autotrophic replacement gilts, 150 middle pigs introduced from other places were attacked from the 2 nd day of the entrance to the 4 th and 13 th days (attack 5 th day), 10 deaths were accumulated, and the swine fever infected in African swine fever was confirmed by clinical examination.
The treatment measures are as follows:
and 4, 13 days, immediately performing harmless treatment on 150 middle pigs introduced from other places.
② 4 months and 13 days, 50 self-reproduction autotrophic replacement gilts which do not show infection symptoms (do not eat) are treated continuously for 14 days (4 months and 13 days-4 months and 26 days in 2019) by using the invention immediately.
The results are reported in the following table:
Figure RE-GDA0002727703730000112
Figure RE-GDA0002727703730000121
blood sample test results
Figure RE-GDA0002727703730000122
Figure RE-GDA0002727703730000123
Figure RE-GDA0002727703730000131
The African swine fever is detected as negative by a national approved third-party detection organization Hunanzhongke gene technology Limited company 42 days after the day of treatment.
Secondly, in order to protect the privacy of the pig farm, part of information related to the pig farm is hidden in an inspection and detection report.
Example 3: pig farm test for 7 days of treatment of African swine fever.
Overview of pig farm: on the 7 th and 3 rd days in 2019, 1 piglet with the weight of less than 50 jin begins to develop in a certain pig farm, and on the 7 th and 7 th days (the 5 th day of the development), the swine fever infected in African is confirmed by clinical examination.
The treatment measures are as follows:
and 7, 7 months and 7 days, all pigs showing infection symptoms (not eating) are subjected to harmless treatment.
② 7 months and 7 days, pigs which do not show infection symptoms (do not eat) are treated for 14-21 days (7 months and 7 days-7 months and 27 days in 2019, wherein the middle and small pigs are treated for 14 days, and the pregnant sows are treated for 21 days) immediately by using the invention.
The results are reported in the following table:
Figure RE-GDA0002727703730000132
example 4: meanwhile, the invention is used for treating swine fever infected in Africa for 3 days and 14 days.
Overview of pig farm: no. 3 and No. 30 in 2019, pigs in a certain small and medium pigsty of a certain pig farm start to get sick, the pig farm is injected with astragalus polysaccharide, cephalosporins, tilmicosin, sulfonamides and other medicaments, no effect is caused, the death rate is increased continuously, and No. 4 and No. 11 (day 12 of the sick) are diagnosed as the African swine fever infected by clinical caesarean section. Here, the group 1 is set.
No. 4 and No. 9 in 2019, pigs in another small and medium pigsty of the pig farm start to develop diseases, the symptoms are consistent with those of the group 1, the pig farm is dare to be treated by any medicine till No. 4 and No. 11 (the disease development day 3), and the swine fever infected with African swine fever is confirmed by clinical examination. Here set as group 2.
The treatment measures are as follows:
no. 4 and No. 11, all pigs showing symptoms (not eating) are subjected to harmless treatment.
② No. 4-11, pigs which do not show symptoms (do not eat) are continuously treated for 14 days (4-11-4-24 days in 2019) by adopting the invention.
The results are reported in the following table:
Figure RE-GDA0002727703730000141
and (3) blood sample detection results:
Figure RE-GDA0002727703730000142
Figure RE-GDA0002727703730000151
Figure RE-GDA0002727703730000152
the African swine fever is detected as negative by a national approved third-party detection organization Hunanzhongke gene technology Limited company 42 days after the day of treatment.
Secondly, in order to protect the privacy of the pig farm, part of information related to the pig farm is hidden in an inspection and detection report.
The invention mainly solves eleven problems of failure in preventing African swine fever, high risk and cost in preventing African swine fever, failure in treating African swine fever, single infection or mixed infection of multiple viral diseases of pigs and the like.
1. Solves the problem of failure in preventing African swine fever: since the onset of African swine fever, many researchers and pig breeders in the world have tried various drugs for preventing African swine fever, but most of them have failed. The success rate of a plurality of pig farms using the invention to prevent African swine fever reaches 100 percent.
2. Solves the problems of high risk and high cost of preventing African swine fever: through the combined efforts of numerous researchers and extensive pig breeders, although some pig farms have been successful in preventing African swine fever through a series of measures such as modifying the pig farms by investing huge amounts of capital and adopting physical prevention and control, disinfection prevention and control and the like, the pig farms bear huge risks and pay expensive prevention cost. The risk of the product is zero when the product is used for preventing the African swine fever, and the product is only required to be added for about 500 yuan per 1 ton of feed, so that the risk and the cost for preventing the African swine fever are greatly reduced.
3. Solves the problem of failure in treating African swine fever: once a pig farm is infected with African swine fever, the most direct prevention and control measure is complete killing. Although some pig farms are actively taking measures for treatment, not only is the treatment cost wasted, but the final mortality rate is almost 100% because there are no safe and effective therapeutic drugs. The application of the invention to the treatment of pig farms which are in close contact with the African swine fever pigs within 5 days but do not show infection symptoms (do not eat), the cure rate reaches 100 percent; the treatment is carried out on a pig farm infected with the African swine fever for 1-3 days, the cure rate is more than 80 percent and can reach 90 percent; the treatment is carried out on a pig farm infected with African swine fever for 4-6 days, the cure rate is more than 70%, and can reach 80%; the pig farm infected with African swine fever for 7-14 days is treated, the cure rate is over 50 percent and can reach 60 percent, and the earlier the pig farm is found, the earlier the pig farm is treated, the higher the cure rate is.
4. Solves the problem of single infection or mixed infection of various viral diseases of pigs: the invention can be used for preventing and treating African swine fever, and also can be used for preventing and treating single infection or mixed infection of viral diseases such as porcine reproductive and respiratory syndrome, porcine circovirus disease, porcine pox, porcine foot and mouth disease, porcine vesicular disease and the like. Is especially used for treating single infection or mixed infection of porcine reproductive and respiratory syndrome and porcine circovirus, and can control death in 5 days and cure in 7 days.
5. Solves the problem that the porcine reproductive and respiratory syndrome and the circovirus disease can not be purified for a long time: according to big data, the porcine reproductive and respiratory syndrome vaccines and the circovirus disease vaccines are inoculated in the pig farm according to the regulations, almost 100% of the pig farm still has the porcine reproductive and respiratory syndrome and the circovirus disease to different degrees, so that the porcine reproductive and respiratory syndrome and the circovirus disease are not purified by any pig farm until now. The pig farm of the invention can not only avoid vaccines of the porcine reproductive and respiratory syndrome and the circovirus disease, but also purify the porcine reproductive and respiratory syndrome and the circovirus disease of the pig farm after continuous use for 180 days.
6. Solves the problem of single infection or mixed infection of various viral diseases of the poultry: the invention can be used for preventing and treating single infection or mixed infection of viral diseases such as low-pathogenicity avian influenza, atypical fowl plague, infectious bursal disease, infectious bronchitis, infectious laryngotracheitis, egg drop syndrome, chicken pox and the like. Particularly, the product of the invention is matched with common antibiotics (for preventing secondary infection of bacteria) to treat infectious bursal disease, the effect is achieved within 24 hours generally, and the death rate is controlled within 48 hours; the product of the invention is used for treating low pathogenic avian influenza, atypical fowl plague or mixed infection of the two by matching with common antibiotics (preventing secondary infection of bacteria), generally takes effect in 48 hours, and controls mortality in 96 hours.
7. Solves the problem of single infection or mixed infection of a plurality of viral diseases of cattle: the invention can prevent single infection or mixed infection of viral diseases such as bovine foot and mouth disease, bovine rotavirus infection, bovine coronavirus infection, bovine viral diarrhea, bovine herpetic stomatitis, bovine infectious rhinotracheitis and the like. Particularly, the product of the invention is matched with common antibiotics (for preventing secondary bacterial infection) to treat single infection or mixed infection of the diseases, and the cure rate is higher than that of the traditional treatment medicines by more than 20 percent.
8. Solves the problem of single infection or mixed infection of various viral diseases of sheep: the invention can be used for preventing viral diseases such as foot-and-mouth disease, sheep pox, sheep aphtha (infectious pustule), bluetongue disease and the like. Particularly, the product of the invention is matched with common antibiotics (for preventing secondary bacterial infection) to treat single infection or mixed infection of the diseases, and the cure rate is higher than that of the traditional treatment medicines by more than 20 percent.
9. Solves the problem of single infection or mixed infection of various viral diseases of aquatic animals: the invention can be used for preventing single infection or mixed infection of viral diseases such as grass carp hemorrhage disease, carp pox herpes disease, channel catfish virus disease, koi herpes virus disease, carp spring viremia, infectious hematopoietic necrosis, viral hemorrhagic septicemia, lymphocystis disease, infectious pancreatic necrosis and the like. Particularly, the product of the invention is matched with common antibiotics (for preventing secondary bacterial infection) to treat single infection or mixed infection of the diseases, and the cure rate is higher than that of the traditional treatment medicines by more than 20 percent.
10. Solves the problems that animal diseases are not easy to cure or relapse easily after being cured: for various reasons, the problem that more and more animal diseases are difficult to cure or easy to relapse after curing has become a common clinical problem. The product of the invention is used as a component of treatment medicine and health care medicine after healing, which not only can reduce the death rate and accelerate the rehabilitation, but also is not easy to relapse after healing.
11. The problems of poor disease resistance and slow growth speed of animals are solved: because the invention can comprehensively improve the nonspecific active immune function of animals, the invention not only can solve the problem of poor disease resistance of animals (the disease incidence is reduced by more than 80 percent, the survival rate is improved by about 5 percent) but also can simultaneously solve the problem of slow growth speed (the growth speed is improved by about 10 percent) after long-term use.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and should not be taken as limiting the invention, and the description should not be taken as limiting the invention, since the scope of the invention will be changed by those skilled in the art according to the spirit of the present invention.

Claims (7)

1. A green composition for preventing and treating African swine fever, which is characterized by comprising the following components in part by weight:
the first microbial formula comprises 20-25 weight percent of: bacillus licheniformis, bacillus subtilis, enterococcus faecalis and saccharomyces cerevisiae;
the second traditional Chinese medicine formula is 70-75 wt%, and comprises: radix astragali, radix Codonopsis, radix Angelicae sinensis, Glycyrrhrizae radix, bupleuri radix, pericarpium Citri Tangerinae, radix Isatidis, flos Lonicerae, fructus crataegi, radix et rhizoma Rhei, and herba Epimedii;
and a third nutritional formula, wherein the weight percentage is 3-6, and the third nutritional formula comprises the following components: vitamin A and vitamin D3Vitamin K3Vitamin B1Vitamin B2Vitamin B6Vitamin B12Vitamin C, nicotinamide, D-calcium pantothenate, folic acid, biotin;
and fully and uniformly stirring all the standby raw materials of the microorganism formula I, the traditional Chinese medicine formula II and the nutrition formula III according to a conventional method.
2. The green composition for preventing and treating African swine fever according to claim 1, wherein the green composition for preventing and treating African swine fever comprises:
the preparation method comprises the following steps of preparing a first microbial formula, namely 53g of bacillus licheniformis, 43g of bacillus subtilis, 33g of enterococcus faecalis and 111g of saccharomyces cerevisiae, wherein the 4 raw materials are fully and uniformly stirred by a conventional method to obtain 240g of the first microbial formula for later use;
700g of astragalus membranaceus, 820g of codonopsis pilosula, 210g of angelica sinensis, 460g of liquorice, 290g of radix bupleuri, 140g of pericarpium citri reticulatae, 300g of radix isatidis, 400g of honeysuckle, 200g of hawthorn, 600g of rheum officinale and 200g of herba epimedii, and the preparation method of the second traditional Chinese medicine formula comprises the following steps of preparing the 11 raw materials into 6: 1, and then fully stirring the mixture evenly according to a conventional method to obtain 720g (crude drug 4320g ÷ 6 ═ 720g) for later use;
nutritional formula III, vitamin A6.15 g and vitamin D31.8g, vitamin K32.2g, vitamin B11g, vitamin B20.6g, vitamin B60.575g and vitamin B120.009g, vitamin C13.8g, nicotinamide 3g, D-calcium pantothenate 2.5g, folic acid 0.2g, biotin 8.166g, the preparation method of the third nutrition formulation is that the 12 raw materials are fully and evenly stirred according to the conventional method, and the total amount is 40g for standby;
fully and uniformly stirring all the standby raw materials of the microorganism formula I, the traditional Chinese medicine formula II and the nutrition formula III according to a conventional method, wherein the total amount of the raw materials is 1000 g.
3. The green composition for preventing and treating African swine fever according to claim 2, wherein the raw materials in the first formula of the microorganism are all 1g containing 1000 hundred million units of bacterial colony.
4. The use of the green composition for preventing and treating African swine fever according to claim 3, wherein the green composition for preventing and treating African swine fever is suitable for normal prevention, emergency prevention and treatment of African swine fever.
5. The use of the green composition for preventing and treating African swine fever according to claim 4, wherein 1000g of the green composition for preventing and treating African swine fever is mixed with 1000 jin of materials or 2000 jin of drinking water and is continuously used until marketing.
6. The use of the green composition for preventing and treating African swine fever according to claim 4, wherein 1000g of the green composition for preventing and treating African swine fever is mixed with 500 jin of materials or 1000 jin of drinking water, and is continuously used for 7 days, and after 7 days, the normal prevention dosage is changed and the green composition is continuously used until marketing.
7. The use of the green composition for preventing and treating African swine fever according to claim 4, wherein the green composition for preventing and treating African swine fever is fed by 5g of piglets, 15g of middle pigs and 25g of big pigs in twice a day, the feeding is carried out for 7 days continuously, half the feeding is carried out for 7 days after 7 days, the emergency prevention amount is fed for 7 days after 7 days, and the normal prevention amount is continuously used for marketing after 7 days after the emergency prevention amount is fed.
CN202010672931.1A 2020-07-14 2020-07-14 Green composition for preventing and treating African swine fever and application thereof Pending CN111956702A (en)

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