CN111919569A - Fertilizing method applied to seedling strengthening of cinnamomum camphora - Google Patents
Fertilizing method applied to seedling strengthening of cinnamomum camphora Download PDFInfo
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- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
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- A—HUMAN NECESSITIES
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- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
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Abstract
The invention discloses a fertilizing method applied to seedling strengthening of cinnamomum camphora, which comprises the following steps: a. selecting experimental cinnamomum leopardi cutting seedlings, and cultivating the seedlings in an experimental place; b. setting influencing factors comprising N, P, K, Fe, Zn and Mg influencing the growth of the seedlings of the cinnamomum camphora; c. setting a control group, wherein no influencing factor influencing the growth of the cinnamomum camphora seedlings in the step b is applied to the control group; d. setting not less than four experimental groups, wherein different quantities of influencing factors influencing the growth of the seedlings of the cinnamomum pantherinum are applied to each experimental group; e. fertilizing once no less than 45 days in all experimental groups, wherein the fertilizing amount of each experimental group is equal; f. after the growth time of the seedlings of the cinnamomum pantherinum is not less than 6 months, equivalently randomly selecting the seedlings of the cinnamomum pantherinum in a control group and an experimental group to determine morphological indexes and physiological indexes; g. and analyzing the measurement result and drawing a conclusion.
Description
Technical Field
The invention relates to the field of plant fertilization amount, in particular to a fertilization method applied to seedling strengthening of cinnamomum camphora.
Background
A Mao Bao Pierre (Litsea coreana var. lanuginosa) is a plant of Litsea of Lauraceae, commonly known as Litsea coreana and white tea [8 ]. According to records of compendium of materia medica, the cinnamomum panthainanense has the efficacies of relieving cough, eliminating phlegm, relieving asthma, relieving summer heat and quenching thirst and the like, and is deeply favored by markets and shows higher drinking value and economic value by the characteristics of pure nature, no pollution, unique flavor and the like of the litsea coreana. In the production of nursery gardens, the cinnamomum pantherinum seedlings have the problems of uneven and thick seedlings, low survival rate of transplanted seedlings, poor seedling resistance and the like, greatly contusion the enthusiasm of planters, and inhibition of further popularization of the cinnamomum pantherinum. With the development of the returning-to-farm engineering, the cinnamomum pantherinum serves as a multipurpose tree species and is positioned as one of the first choice tree species for returning to the farm.
Disclosure of Invention
Aiming at the problems, the invention provides a fertilizing method applied to the strong seedlings of the cinnamomum koehmannii, which has the advantages of improving the quality of cinnamomum koehmannii seedlings and improving the current production situation.
The technical scheme of the invention is as follows:
a fertilizing method applied to seedling strengthening of cinnamomum camphora, comprising the following steps:
a. selecting experimental cinnamomum leopardi cutting seedlings, and cultivating the seedlings in an experimental place;
b. setting experimental factors comprising N, P, K, Fe, Zn and Mg for influencing the growth of the seedlings of the cinnamomum camphora;
c. setting a control group, wherein the control group is not applied with the experimental factors influencing the growth of the cinnamomum pantherinum seedlings in the step b;
d. setting not less than four experimental groups, wherein different amounts of experimental factors influencing the growth of the seedlings of the cinnamomum pantherinum are applied to each experimental group;
e. fertilizing once no less than 45 days in all experimental groups, wherein the fertilizing amount of each experimental group is equal;
f. after the growth time of the seedlings of the cinnamomum pantherinum is not less than 6 months, equivalently randomly selecting the seedlings of the cinnamomum pantherinum in a control group and an experimental group to determine morphological indexes and physiological indexes;
g. and analyzing the measurement result and drawing a conclusion.
The working principle of the technical scheme is as follows:
the test material is annual Litsea cubeba seedling cutting seedling, N, P, K, Fe, Zn and Mg are set, 6 factor 5 level, and L is adopted25(56) The orthogonal design is used for fertilizing, the total number of the fertilizing units is 25, and 50 plants and 1250 plants are arranged in each treating unit. The fertilizers used in the test are urea, calcium magnesium phosphate, potassium chloride, ferrous sulfate heptahydrate, magnesium sulfate heptahydrate and zinc sulfate heptahydrate; adopting average fertilization, ditching among rows of the processing unit for furrow application, applying fertilizer for the first time at the bottom of 3 months every year, applying fertilizer once every 45 days, and applying fertilizer for 4 times; randomly extracting 1 seedling in each processing unit at the end of 9 months, obtaining 25 seedlings in total, and measuring morphological index (seedling height, ground diameter, lateral root length, overground part dry mass and root dry mass) and physiological index (soluble sugar content, soluble protein content, chlorophyll total content and total flavone) of the seedlings; through the method, the influence factors (namely the optimal dosage of N fertilizer, P fertilizer, K fertilizer, Fe fertilizer, Zn fertilizer and Mg fertilizer) of the growth of the cuttage seedlings of the cinnamomum camphora seedlings which are most suitable for one year can be measured.
In a further technical scheme, in the step a, an annual leopard cinnamomum camphora cutting seedling is selected, and the soil physicochemical property of an experimental land is as follows: purple soil, pH 6.6, water alkaline nitrogen 93.26mg/kg, quick-acting potassium 53.50mg/kg and available phosphorus 40.17 mg/kg.
In a further technical scheme, each experimental group and the control group respectively form a treatment unit, and the number of seedlings in each treatment unit is more than or equal to 50.
In a further technical scheme, the distance between every two processing units is more than or equal to 20cm, and the distance between every two seedlings in every processing unit is more than or equal to 5 cm.
In a further technical scheme, the fertilizing amount of each seedling in the first experimental group in the step d is as follows: n fertilizer: 5 parts of fertilizer P: 5 parts of K fertilizer: 5 parts of Fe fertilizer: 1 part and Zn fertilizer: 1 part of Mg fertilizer: 1 part;
the fertilizing amount of each seedling in the second experimental group is twice of that of the first experimental group; the fertilizing amount of each seedling in the third experimental group is three times of that of the first experimental group; the amount of fertilizer applied per seedling in the fourth experimental group was four times that in the first experimental group.
In a further technical scheme, the N fertilizer is urea, and the P fertilizer is calcium magnesium phosphate; the K fertilizer is potassium chloride, and the Fe fertilizer is ferrous sulfate heptahydrate; the Zn fertilizer is zinc sulfate heptahydrate; the Mg fertilizer is magnesium sulfate heptahydrate.
In a further technical scheme, in the step f, a seedling is randomly selected from each processing unit.
In a further technical scheme, in the step f, the measured seedling morphology indexes comprise seedling height, ground diameter, lateral root length, overground part dry mass and root dry mass; the physiological indexes of the seedlings are measured to comprise soluble sugar content, soluble protein content, chlorophyll total content and total flavone.
In a further technical scheme, in the step g, the test data are simply sorted, statistically analyzed and graphed by using Microsoft Excel and SPSS, the data among seedlings are subjected to multiple comparisons by using a Duncan method, and the variance analysis and range analysis are performed by using a general linear statistical model.
The invention has the beneficial effects that:
1. the test material is annual Litsea cubeba seedling cutting seedling, N, P, K, Fe, Zn and Mg are set, 6 factor 5 level, and L is adopted25(56) Orthogonal design for fertilizing treatment, 25 sitesAnd the number of the treatment units is 50, and the total number is 1250. The fertilizers used in the test are urea, calcium magnesium phosphate, potassium chloride, ferrous sulfate heptahydrate, magnesium sulfate heptahydrate and zinc sulfate heptahydrate; adopting average fertilization, ditching among rows of the processing unit for furrow application, applying fertilizer for the first time at the bottom of 3 months every year, applying fertilizer once every 45 days, and applying fertilizer for 4 times; randomly extracting 1 seedling in each processing unit at the end of 9 months, obtaining 25 seedlings in total, and measuring morphological index (seedling height, ground diameter, lateral root length, overground part dry mass and root dry mass) and physiological index (soluble sugar content, soluble protein content, chlorophyll total content and total flavone) of the seedlings; by the method, the influence factors (namely the optimal dosage of N fertilizer, P fertilizer, K fertilizer, Fe fertilizer, Zn fertilizer and Mg fertilizer) of the growth of the cuttage seedlings of the cinnamomum camphora seedlings which are most suitable for one year can be measured;
2. under different soil conditions, the quantity of the applied influencing factors is different, so that the quantity of the corresponding influencing factors can be applied according to the field situation after various soil conditions are detected during testing;
3. the experimental group and the control group which are applied with the same score influence factors are respectively provided with a processing unit, so that the representativeness of the sample can be increased;
4. the morphological index and the physiological index measured by the extracted sample seedling reflect the growth state of the seedling according to various data, and directly reflect the influence of the quantity of the influencing factors on the growth state of the seedling.
Detailed Description
The following examples further illustrate the invention.
Example (b):
a fertilizing method applied to seedling strengthening of cinnamomum camphora, comprising the following steps:
a. selecting experimental cinnamomum leopardi cutting seedlings, and cultivating the seedlings in an experimental place;
b. setting influencing factors comprising N, P, K, Fe, Zn and Mg influencing the growth of the seedlings of the cinnamomum camphora;
c. setting a control group, wherein no influencing factor influencing the growth of the cinnamomum camphora seedlings in the step b is applied to the control group;
d. setting not less than four experimental groups, wherein different quantities of influencing factors influencing the growth of the seedlings of the cinnamomum pantherinum are applied to each experimental group;
e. fertilizing once no less than 45 days in all experimental groups, wherein the fertilizing amount of each experimental group is equal;
f. after the growth time of the seedlings of the cinnamomum pantherinum is not less than 6 months, equivalently randomly selecting the seedlings of the cinnamomum pantherinum in a control group and an experimental group to determine morphological indexes and physiological indexes;
g. and analyzing the measurement result and drawing a conclusion.
In another embodiment:
in the step a, annual leopard cinnamomum camphora cutting seedlings are selected, and the soil physicochemical property of the experimental land is as follows: purple soil, pH 6.6, water alkaline nitrogen 93.26mg/kg, quick-acting potassium 53.50mg/kg and available phosphorus 40.17 mg/kg.
In another embodiment:
the control group and each experimental group are respectively provided with five groups, each group is provided with one processing unit, and the number of seedlings in each processing unit is more than or equal to 50.
In another embodiment:
the distance between every two processing units is more than or equal to 20cm, and the distance between every two seedlings in every processing unit is more than or equal to 5 cm.
In another embodiment:
the fertilizing amount of each seedling in the first experiment group in the step d is as follows: n fertilizer: 5 parts of fertilizer P: 5 parts of K fertilizer: 5 parts of Fe fertilizer: 1 part and Zn fertilizer: 1 part of Mg fertilizer: 1 part;
the fertilizing amount of each seedling in the second experimental group is twice of that of the first experimental group; the fertilizing amount of each seedling in the third experimental group is three times of that of the first experimental group; the amount of fertilizer applied per seedling in the fourth experimental group was four times that in the first experimental group.
In another embodiment:
the N fertilizer is urea, and the P fertilizer is calcium magnesium phosphate; the K fertilizer is potassium chloride, and the Fe fertilizer is ferrous sulfate heptahydrate; the Zn fertilizer is zinc sulfate heptahydrate; the Mg fertilizer is magnesium sulfate heptahydrate.
In another embodiment:
in step f, a seedling is randomly selected from each processing unit.
In another embodiment:
in the step f, determining the shape indexes of the seedlings, including the height of the seedlings, the ground diameter, the lateral root length, the dry mass of the overground part and the dry mass of the roots; the physiological indexes of the seedlings are measured to comprise soluble sugar content, soluble protein content, chlorophyll total content and total flavone.
In another embodiment:
in the step g, the test data are simply collated, statistically analyzed and graphed by using Microsoft Excel and SPSS, the data among seedlings are subjected to multiple comparisons by using a Duncan method, and variance analysis and range analysis are performed by using a general linear statistical model.
The specific experimental mode is as follows:
in the step a, selecting annual leopard cinnamomum camphora cutting seedlings, experimentally selecting the seedlings in Zhongzhou town (E: 102 degrees, 51-103 degrees, 12 degrees and N: 29 degrees, 40-30 degrees, 14 degrees) of Yaan city, Sichuan province, at the west edge of Sichuan basin, in the midstream of Qingyi river, in the transition zone from Chengdu plain to Qinghai-Tibet plateau, in subtropical climate zones, with the annual precipitation amount of 1000-1800 mm and the annual average temperature of 14-16 ℃; basic physicochemical properties of the soil to be tested: purple soil, pH 6.6, water alkaline nitrogen 93.26 mg/kg-1, quick-acting potassium 53.50 mg/kg-1 and available phosphorus 40.17 mg/kg-1; the method adopts a variable control method to carry out the test, selects the condition suitable for the growth of the fur leopard cinnamomum camphora, and controls the influence of the climate condition and the soil condition on the fur leopard cinnamomum camphora under the same change to carry out the test.
Respectively arranging five groups of a control group and each experimental group, wherein each group is arranged as a processing unit, the number of seedlings in each processing unit is 50, the total number is 1250, the row spacing and the line spacing of each plant are 5cm and 7cm, and the spacing between the processing units is 20 cm; set up reasonable row spacing and row interval, can guarantee that every seedling can both receive sufficient illumination and rainwater, the influence factor diffusion to adjacent processing unit that exerts can be avoided to the interval between the great processing unit.
The fertilizers used in the test are urea (containing 46 percent of N element) and calcium magnesium phosphate (containing P)2O512 percent of potassium chloride (containing K)260% of O), 20.14% of ferrous sulfate heptahydrate (containing Fe element), 9.76% of magnesium sulfate heptahydrate (containing Mg element), 22.65% of zinc sulfate heptahydrate (containing Zn element), and applying corresponding fertilizers according to the element weight in the table 1 to each different treatment unit; adopting average fertilization, ditching among rows of the processing unit for furrow application, applying fertilizer for the first time at the bottom of 3 months, applying fertilizer once every 45 days, and applying fertilizer for 4 times;
TABLE 1L25(56) Orthogonality test factors and levels
Tab.1 The factors and levels of L25(56)orthogonal experiment design
Note that in the table, the levels of N, P, K, Mg, Zn and Fe are the element dosage;
randomly extracting 1 seedling from each processing unit at the end of 9 months in the same year, dividing the total number of the seedlings into five groups according to the quantity of influence factors exerted by experiments, and measuring the morphological indexes of the seedlings, including the height of the seedlings, the ground diameter, the lateral root length, the dry mass of the overground part and the dry mass of the roots; measuring physiological indexes of seedlings including soluble sugar content, soluble protein content, chlorophyll total content and total flavone;
the method comprises the following steps of carrying out simple arrangement, statistical analysis and chart making on test data by using Microsoft Excel and SPSS, carrying out multiple comparison on data among seedlings by using a Duncan method, and carrying out variance analysis and range analysis by using a general linear statistical model;
the influence of the influencing factors on the shape indexes of the cinnamomum camphora is as follows:
influence of influencing factors on seedling height of cinnamomum camphora seedlings:
as can be seen from Table 2, the average height of the seedlings of the cinnamomum camphora after the influence factor treatment is 7.5-13.71 cm; the seedling height of each treatment unit, except for treatment units 14 and 18, increased significantly after the influencing factor was applied, the differences between treatment units 4, 5, 8, 11, 16, 21, 23 and 25 were insignificant, but the seedling height was significantly higher than in the control group; the heights of the seedlings treated by the treatment units 2, 3, 7, 10, 19, 20, 6, 12, 13, 17, 9, 15, 22 and 24 are not different from those of the control group, but are all higher than those of the control group; treatment unit 14 and treatment unit 18 were not significantly different from the control group, both slightly lower than the control; the overall explanation shows that the application of influencing factors has a promoting effect on the height of seedlings;
TABLE 2 influence of fertilization on the morphological index of Cinnamomum robustum
Tab.2 The effects of fertilization on morphological indices of L.coreana var.lanuginosa
Note that different lower case letters indicate that each index is significantly different at the 5% level.
From tables 3 and 4, it can be seen that the N element and the K element have significant (P <0.05) influence on the seedling height of the annual cutting seedling of cinnamomum camphora, and the P element, the Mg element, the Zn element and the Fe element have no significant (P >0.05) influence on the seedling height of cinnamomum camphora; the influence of different influence factors on the height of the seedling can be obtained from the range value to obtain a preliminary conclusion; the larger the range, the larger the effect on the height growth of seedlings.
As can be seen from Table 3, the sequence of the magnitude of the analytical range difference of the seedling heights of the six influencing factors, namely the sequence of the influence of the six influencing factors on the seedling height of the annual cutting seedling of the cinnamomum camphora is K > N > Mg > P > Fe > Zn, and by comparing the mean values of all levels (K1, K2, K3, K4 and K5), the theoretically optimal fertilization combination for the height growth of the annual cutting seedling of the cinnamomum camphora is the processing unit 25, namely 8.59 g/plant of urea, 7.69 g/plant of calcium magnesium phosphate fertilizer, 5.74 g/plant of potassium chloride, 4.10 g/plant of magnesium sulfate heptahydrate and 0.88 g/plant of zinc sulfate heptahydrate.
TABLE 3 range analysis of morphological indices
Tab.3 morphology of the range analysis
Note that K1-K5 represent the mean values of the different levels and R represents the difference values.
TABLE 4 analysis of the principal Effect variance of morphological indices
Tab.4 morphology of between-subjects effects
Note:indicatessignificance at the 0.05 level.
The influence factors influence the soluble sugar content of the leaves of the cinnamomum camphora seedlings:
from table 5, the lowest soluble sugar content of the control group after fertilization treatment was 11.011mg/g, which indicates that the application of influencing factors has a positive effect on increasing the soluble sugar content of annual cutting seedlings of cinnamomum camphora leopard; the soluble sugar content of treatment units 6, 10, 12 and 25 did not differ significantly from the control group, and there were significant differences between the remaining treatments and the control group, with treatment unit 4 and treatment unit 19 differing most significantly, 25.756 mg/g and 26.664mg/g, respectively, and 14.745mg/g and 15.653mg/g, respectively, from the control group.
TABLE 5 influence of fertilization on the content index of Cinnamomum robustum
Tab.5 The effects of fertilization on contents indices of L.coreana var.lanuginosa
Note that the difference in the level of 5% for different lower case letters is significant.
From tables 6 and 7, it can be seen that the application of the three influencing factors of the element N, the element P and the element Mg has a significant influence on the soluble sugar content of the annual cutting seedlings of the cinnamomum camphora (P <0.05), and the application of the three influencing factors of the element K, the element Zn and the element Fe has an insignificant influence on the soluble sugar content of the annual cutting seedlings of the cinnamomum camphora (P > 0.05). The effect of different influencing factors on soluble sugars can lead to preliminary conclusions from the magnitude of the range. The greater the range, the greater its effect on soluble sugars. As can be seen from table 6, the order of the magnitude of the very different values of the soluble sugar analysis of the six factors, that is, the order of the magnitude of the influence of the six influencing factors on the soluble sugar content of the annual cutting seedlings of the cinnamomum pantherinum, is: n > Mg > P > Fe > Zn > K, and the growth of soluble sugar of annual cutting seedlings of the cinnamomum leoparum is theoretically optimally combined into a treatment unit 4 by comparing the mean values of all levels (K1, K2, K3, K4 and K5), namely 5.77 g/plant of calcium magnesium phosphate fertilizer, 5.74 g/plant of potassium chloride, 6.15 g/plant of magnesium sulfate heptahydrate, 2.98 g/plant of ferrous sulfate heptahydrate and 2.64 g/plant of zinc sulfate heptahydrate.
Table 6 analysis of the main effect variance of the inclusion
Tab.6 contents of between-subjects effects
Note:indicatessignificance at the 0.05 level.
Table 7 analysis of the main effect variance of the inclusion
Tab.7 contents of the range analysis
Note that K1-K5 represent the mean values of the different levels and R represents the difference values.
The influence factors influence the soluble protein content of the leaves of the cinnamomum camphora seedlings by:
as can be seen from table 5, there was no significant difference between the soluble protein content of the remaining treatment units and the control, except that treatment unit 19 had a significant difference in soluble protein content from the control. Wherein the soluble protein content of treatment units 2, 15 and 24, treatment units 3, 6 and 16, treatment units 5, 18 and 22, and treatment unit 21 was not significantly different from the control group, but was higher than the control group.
From tables 6 and 7, it can be seen that three influencing factors of the K element, the Mg element and the Fe element have significant influence on the soluble protein of the annual cutting seedling of the cinnamomum parviflorum (P <0.05), and three influencing factors of the N element, the P element and the Zn element do not have significant influence on the content of the soluble protein (P > 0.05). Similarly, the order of the influence of the factors on the content of the soluble protein can be known by comparing the range of the influence factors: fe > K > Mg > Zn > P > N, wherein the influence of Fe element is the largest, and through the average comparison of all levels, the growth theory of the content of soluble protein of the annual cutting seedling of the cinnamomum camphora leoparum is the best treatment element 19, namely 6.44g of urea, 5.77g of calcium magnesium phosphate fertilizer, 1.91g of potassium chloride, 6.15g of magnesium sulfate heptahydrate, 2.98g of ferrous sulfate heptahydrate and 2.64g of zinc sulfate heptahydrate.
Influence factors influence the chlorophyll content of leaves of cinnamomum camphora seedlings:
the total chlorophyll amount can reflect the photosynthesis intensity, and as can be seen from table 5, the chlorophyll content of the treatment unit 22 is the lowest and is 1.148mg/g, the chlorophyll contents of the treatment units 12, 14, 16, 20 and 22 are not significantly different from the control group, and the remaining treatment units are significantly different from the control group. The treatment units 12, 22 and the control group were at the same level. Except the treatment unit 22, the chlorophyll content of the other treatment units is higher than that of the control group, which indicates that the application of influencing factors has a promoting effect on the chlorophyll content of the annual cutting seedlings of the cinnamomum pantherinum.
As can be seen from tables 6 and 7, the chlorophyll content of the annual cutting seedling of the cinnamomum pantherinum is significantly influenced by N element, P element, K element, Mg element, Zn element and Fe element (P <0.05), and the influence sequence of each influencing factor is as follows through range comparison of each influencing factor: k is the most affected, the influence of K elements is the most, the optimal fertilization combination of the annual cutting seedling chlorophyll content growth theory of the cinnamomum leoparum can be obtained by combining the average values of all levels, and the optimal fertilization combination is the processing unit 2, namely 1.92 g/plant of calcium magnesium phosphate fertilizer, 1.91 g/plant of potassium chloride, 8.20 g/plant of magnesium sulfate heptahydrate, and 1.77 g/plant of zinc sulfate heptahydrate.
The fertilization has influence on the flavone content of the leaves of the cinnamomum camphora seedlings:
the flavonoids widely exist in the plant world, are substances with various physiological and pharmaceutical effects, have the effects of improving blood circulation, reducing blood pressure, reducing blood sugar and resisting cancer, and can be seen from table 5 that the treatment units 3, 10 and 13, the treatment unit 5 and the treatment units 14 and 16 have larger difference between the flavone content of the treatment 15 and the content of the control group, wherein the treatment units 3, 10 and 13 are obviously higher than the control group, and the treatment units 5, 14 and 16 have the flavone content of the treatment 15 which is obviously lower than the control group. The treatment units 2, 6, 7, 9, 17, 20 and 25, the treatment unit 11, the flavone content and the control group have no great difference, but are all higher than the control group, and the rest treatment units have no significant difference with the control group and are lower than the control group.
From table 6 and table 7, it can be seen that N, P, K, Mg, Zn, and Fe all affect the flavone content of the annual cutting seedling of sassafras pilosella leopard significantly (P <0.05), and from the extreme differences, the order of the effects of each influencing factor is as follows: zn, Mg, P, K, Fe and N, and the average value of all levels shows that the optimal combination of the annual cutting seedling flavone content and the growth of the cinnamomum leoparum is the processing unit 2, namely 1.92 g/plant of calcium magnesium phosphate fertilizer, 1.91 g/plant of potassium chloride, 2.05 g/plant of magnesium sulfate heptahydrate, 0.99 g/plant of zinc sulfate heptahydrate and 0.88 g/plant of zinc sulfate heptahydrate.
And (4) conclusion:
different quantities of influence factors are applied to cause the difference of various indexes of annual cutting seedlings of the cinnamomum camphora leopard among different treatments, which shows that the application of the influence factors has great influence on the growth of the cutting seedlings. Among the 25 treatments in the present invention, some treatments were not effective, and it is important to explain the ratio of the influencing factors.
The influence of fertilization on the morphological indexes of the cinnamomum camphora:
the result analysis shows that the influence factor N has significant influence on the seedling height, the ground diameter, the lateral root length and the biomass content of the cinnamomum camphora seedlings (P <0.05), the influence factor P has significant influence on the lateral root length and the dry weight of the underground part (P <0.05), the influence factor K has significant influence on the seedling height, the lateral root length and the dry weight of the underground part (P <0.05), the influence factor Mg has no significant influence on the form index (P >0.05), the influence factor Zn has significant influence on the lateral root length and the dry weight of the underground part (P <0.05), and the influence factor Fe has significant influence on the lateral root length and the biomass (P <0.05), which indicates that the influence factor N has the greatest influence on the plants.
The P element is a main nutrient element indispensable to the growth and development of plants, is a composition of an important organic compound in the bodies of crops, and participates in physiological processes in the bodies of the plants in multiple ways. The P element is an indispensable element for forming cell nucleus protein, lecithin and the like, and can accelerate cell division and promote the growth of root systems and overground parts. When the P element is lacked, the root system of the plant firstly feels stress, and the result of the invention shows that the lateral root length of the cinnamomum pantherinum is increased along with the increase of the application of the P element which is an influencing factor.
During the growth and development of plants, K is involved in the processes of activation of more than sixty enzyme systems, photosynthesis, transport of assimilation products, carbohydrate metabolism, protein synthesis and the like. The mean value comparison of different levels shows that the seedling height, the lateral root length and the dry weight of the overground part of the cinnamomum kombuci hayata are increased and then decreased along with the increase of the horizontal concentration of the K element, which indicates that the K element has a promoting effect on the growth of the cinnamomum kombuci hayata within a certain concentration range, but the inhibition phenomenon can occur beyond the range.
Zn element is one of the essential trace elements of plants. The invention shows that with the increase of the application amount of Zn element which is an influencing factor, the lateral root length and the biomass of the underground part of the cinnamomum pantherinum show the trend of increasing first and then decreasing.
Fe is one of essential nutrient elements of plants, and one of physiological functions of Fe is participating in oxidation-reduction reaction and electron transfer in plant cells. The invention shows that the side root length and the biomass of the cinnamomum pantherinum show the trend of increasing firstly and then decreasing with the increase of the application amount of the iron fertilizer, and the increase of the iron fertilizer in a certain concentration has the effect of promoting the growth of the cinnamomum pantherinum.
Combining with each index analysis, the optimal combination of the height and the ground diameter growth of the cinnamomum leopardi seedlings is a processing unit 25; the optimal combination of the lateral root length is the processing unit 15, the lateral root length of the processing unit 25 is not obviously different from that of the processing unit 15, and meanwhile, the two processing units are obviously higher than the control group; the optimal combination of the dry weight of the aerial parts is the processing unit 4, the dry weight of the aerial parts of the processing unit 25 has no significant difference with the dry weight of the aerial parts of the processing unit 4, and the dry weights are all higher than that of the control group; the other treatment units of the underground part dry weight have no obvious difference with the control group, the underground biomass of the treatment unit 25 is higher than that of the control group, and comprehensively, the optimal influencing factors of the shape index of the leopard skin camphor are the treatment unit 25, namely 8.59 g/strain of urea, 7.69 g/strain of calcium magnesium phosphate fertilizer, 5.74 g/strain of potassium chloride, 4.10 g/strain of magnesium sulfate heptahydrate and 0.88 g/strain of zinc sulfate heptahydrate.
Influence of fertilization on the content of the cinnamomum camphora content:
soluble sugar, soluble protein and chlorophyll can be used as indexes of cold resistance of plants. The result analysis of the invention shows that the influencing factors N and P have obvious influence on the content of the soluble sugar, the total chlorophyll and the flavone of the leopard cinnamomum camphora (P <0.05), the influencing factors K and Fe have obvious influence on the content of the soluble protein, the total chlorophyll and the flavone (P <0.05), the influencing factor Mg has obvious influence on the indexes of four inclusions (P <0.05), and the influencing factor Zn only has obvious influence on the content of the chlorophyll and the flavone of the leopard cinnamomum camphora (P < 0.05).
The result of the invention shows that the increase of the influence factor N element in a certain range can increase the content of soluble sugar and the content of chlorophyll in the cinnamomum pantherinum, and can effectively improve the cold resistance of the cinnamomum pantherinum. Under the treatment of different influencing factor N element levels, the total flavone content in the seedlings of the cinnamomum pantherinum is different, the content is the maximum content in a control group, the flavone content is gradually reduced along with the increase of the influencing factor N element level, the increase of the influencing factor N element level promotes the increase of biomass, nutrient elements for promoting the generation of flavone are diluted, and the synthesis of flavone is limited.
The influencing factor P can promote root development and improve cold resistance and drought resistance of trees. The invention shows that the application of the influencing factor P has obvious influence on the soluble sugar content and the total chlorophyll content of the cinnamomum pantherinum, and the soluble sugar content and the total chlorophyll content of the cinnamomum pantherinum after the influencing factor P is treated are substantially higher than those of a control group; the flavone content of the cinnamomum pantherinum increases firstly and then decreases along with the increase of the level of the P element which is an influencing factor, and reaches the maximum value at the K3 level of phosphorus, namely 2 g/strain.
The influencing factor K is an activator of various enzymes, promotes photosynthesis, participates in the synthesis, transportation and transformation of sugar and starch, and participates in the synthesis of protein. The invention shows that the influencing factor K has obvious influence on the content of soluble protein, chlorophyll total amount and flavone of the cinnamomum pantherinum (P is less than 0.05). Along with the increase of the level of the element K of the application influencing factor, the content of soluble protein and the total content of chlorophyll of the cinnamomum pantherinum show the trend of rising firstly and then falling, which shows that the potassium fertilizer is suitable for being applied to promote the growth and development of the cinnamomum pantherinum and improve the quality of plants.
The invention shows that the content of soluble sugar, soluble protein and chlorophyll of the leopard cinnamomum camphora is increased by the level of the influencing factor Mg, and shows that the stress resistance of the leopard cinnamomum camphora can be promoted by the increase of the influencing factor Mg with low level. According to the invention, with the increase of the amount of Mg element which is an influencing factor, the flavone content of the leaf of the cinnamomum pantherinum shows an overall decreasing trend.
Experiments show that the soluble protein content and the total chlorophyll content of the cinnamomum pantherinum can be improved by the aid of the influence factor Fe element in a certain concentration range. The content of flavone in the leaves of the cinnamomum pantherinum is increased and then reduced along with the increase of the level of the Fe element, which is an influencing factor, and the influence factor Fe element has a promoting effect on the content of the leaves of the cinnamomum pantherinum within a certain range.
The invention shows that the total content of the chlorophyll of the cinnamomum pantherinum shows a trend of increasing and then reducing along with the increase of the affecting factor Zn element, and the flavone of the cinnamomum pantherinum leaves also shows a trend of increasing and then reducing along with the increase of the level of the affecting factor Zn element, thereby showing that the affecting factor Zn element with low concentration promotes the synthesis of the flavone of the leaves, and the affecting factor Zn element with high concentration can become one of the reasons for limiting the synthesis of the flavone.
Combining with each index analysis, the optimal combination of the gross amount of chlorophyll of the leopard cortex cinnamomi camphora and the content of flavone in the leaves is a processing unit 2; the optimal combination of soluble sugars is the processing unit 4, and the soluble proteins of the processing unit 2 and the processing unit 4 have no significant difference and are all significantly higher than those of the control group; the optimal combination of soluble proteins is treatment unit 19, and the soluble proteins in treatment unit 2 and the soluble proteins in treatment unit 19 have no significant difference and are all higher than those in the control group. In conclusion, the optimal fertilization combination for the content index of the cinnamomum camphora leoparum is the treatment unit 2, namely 1.92 g/plant of calcium magnesium phosphate fertilizer, 1.91 g/plant of potassium chloride, 2.05 g/plant of magnesium sulfate heptahydrate, 1.99 g/plant of zinc sulfate heptahydrate and 0.88 g/plant of zinc sulfate heptahydrate.
In conclusion, the optimal application influencing factors for the shape indexes of the cinnamomum camphora serve as the processing unit 25, the optimal fertilization of the inclusions serves as the processing unit 2, and in agricultural production, farmers can use the processing unit 25 to apply the influencing factors to serve as base fertilizer, so that the seedlings grow fast, the processing unit 2 is used for applying fertilizer when the seedlings grow vigorously in the later stage of seedling growing, the inclusions of the seedlings are increased, and the stress resistance of the seedlings is improved. The comprehensive fertilization can not only cultivate high-quality nursery stocks in a planned and purposeful way, but also greatly save the cost in production and improve the economic benefit of farmers.
The above-mentioned embodiments only express the specific embodiments of the present invention, and the description thereof is more specific and detailed, but not construed as limiting the scope of the present invention. It should be noted that, for a person skilled in the art, several variations and modifications can be made without departing from the inventive concept, which falls within the scope of the present invention.
Claims (9)
1. A fertilizing method applied to seedling strengthening of cinnamomum camphora, which is characterized by comprising the following steps:
a. selecting experimental cinnamomum leopardi cutting seedlings, and cultivating the seedlings in an experimental place;
b. setting experimental factors comprising N, P, K, Fe, Zn and Mg for influencing the growth of the seedlings of the cinnamomum camphora;
c. setting a control group, wherein the control group is not applied with the experimental factors influencing the growth of the cinnamomum pantherinum seedlings in the step b;
d. setting not less than four experimental groups, wherein different amounts of experimental factors influencing the growth of the seedlings of the cinnamomum pantherinum are applied to each experimental group;
e. fertilizing once no less than 45 days in all experimental groups, wherein the fertilizing amount of each experimental group is equal;
f. after the growth time of the seedlings of the cinnamomum pantherinum is not less than 6 months, equivalently randomly selecting the seedlings of the cinnamomum pantherinum in a control group and an experimental group to determine morphological indexes and physiological indexes;
g. and analyzing the measurement result and drawing a conclusion.
2. The fertilizing method applied to the young seedlings of the cinnamomum pantherinum according to claim 1, wherein in the step a, annual young seedlings of the cinnamomum pantherinum are selected, and the physicochemical properties of experimental soil are as follows: purple soil, pH 6.6, water alkaline nitrogen 93.26mg/kg, quick-acting potassium 53.50mg/kg and available phosphorus 40.17 mg/kg.
3. The fertilizing method applied to the seedlings of cinnamomum pantherinum according to claim 1, wherein each experimental group and the control group form a treatment unit, and the number of seedlings in each treatment unit is greater than or equal to 50.
4. The fertilizing method applied to young seedlings of Cinnamomum pantherinum according to claim 3, wherein the distance between each processing unit is greater than or equal to 20cm, and the distance between each seedling in each processing unit is greater than or equal to 5 cm.
5. The fertilizing method applied to the young seedlings of cinnamomum pantherinum according to claim 1, wherein the fertilizing amount per seedling in the first experimental group in the step d is as follows: n fertilizer: 5 parts of fertilizer P: 5 parts of K fertilizer: 5 parts of Fe fertilizer: 1 part and Zn fertilizer: 1 part of Mg fertilizer: 1 part;
the fertilizing amount of each seedling in the second experimental group is twice of that of the first experimental group; the fertilizing amount of each seedling in the third experimental group is three times of that of the first experimental group; the amount of fertilizer applied per seedling in the fourth experimental group was four times that in the first experimental group.
6. The fertilizing method applied to the seedlings and the strong seedlings of cinnamomum pantherinum according to claim 5, wherein the N fertilizer is urea, and the P fertilizer is calcium magnesium phosphate; the K fertilizer is potassium chloride, and the Fe fertilizer is ferrous sulfate heptahydrate; the Zn fertilizer is zinc sulfate heptahydrate; the Mg fertilizer is magnesium sulfate heptahydrate.
7. The fertilizing method applied to the young seedlings of cinnamomum pantherinum according to claim 5, wherein in step f, a seedling is randomly selected from each processing unit.
8. The fertilizing method applied to the young seedlings of cinnamomum pantherinum according to claim 1, wherein in the step f, the measured seedling morphology indexes comprise seedling height, ground diameter, lateral root length, overground part dry mass and root dry mass; the physiological indexes of the seedlings are measured to comprise soluble sugar content, soluble protein content, chlorophyll total content and total flavone.
9. The fertilizing method for young seedlings of cinnamomum pantherinum according to any one of claims 1 to 8, wherein in the step g, Microsoft Excel and SPSS are used for simple arrangement, statistical analysis and charting of test data, multiple comparisons of data among seedlings are performed by a Duncan method, and analysis of variance and range analysis are performed by a general linear statistical model.
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Application publication date: 20201113 |