CN111893030B - Solid-state fermentation liquid strain inoculation device - Google Patents
Solid-state fermentation liquid strain inoculation device Download PDFInfo
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- CN111893030B CN111893030B CN202010793121.1A CN202010793121A CN111893030B CN 111893030 B CN111893030 B CN 111893030B CN 202010793121 A CN202010793121 A CN 202010793121A CN 111893030 B CN111893030 B CN 111893030B
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M21/00—Bioreactors or fermenters specially adapted for specific uses
- C12M21/16—Solid state fermenters, e.g. for koji production
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/46—Means for fastening
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/48—Holding appliances; Racks; Supports
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/52—Mobile; Means for transporting the apparatus
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
- C12M27/10—Rotating vessel
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M37/00—Means for sterilizing, maintaining sterile conditions or avoiding chemical or biological contamination
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
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Abstract
The invention discloses a solid fermentation liquid strain inoculation device, and relates to the technical field of solid fermentation equipment; the upper side of the inside of the box body is provided with a feeding mechanism, the upper end of a telescopic pipe in the feeding mechanism is connected with the lower end of a discharge pipe, the lower side of the feeding mechanism is provided with a fixing mechanism, the center of the lower surface of a placing plate in the fixing mechanism is fixedly connected with a rotating rod in the rotating mechanism, the lower end of the rotating rod is screwed with the lower side wall of the box body through a bearing, the box body is of a front side open structure, the front side of the box body is internally provided with a door body, the left side and the right side of the middle side of the door body are respectively provided with a through hole, rubber gloves are arranged in the through holes, and the outer periphery of the top ends of the rubber gloves is fixedly connected with the inner peripheral wall of the through holes; before the fermentation cylinder cools off to certain temperature, will place the fermentation cylinder inside this device, after its cooling is accomplished, add the fermentation cylinder with the microorganism bacterial again in, need not to shift, directly rock the fermentation cylinder for the speed of mixing, labour saving and time saving when being difficult for contacting other bacteria.
Description
Technical Field
The invention relates to the technical field of solid-state fermentation equipment, in particular to a solid-state fermentation liquid strain inoculation device.
Background
The solid state fermentation is a process of culturing microorganisms by using an insoluble solid matrix, and is a submerged fermentation process of suspending the solid state in liquid, wherein in the fermentation production, a certain amount of pure microorganism strains are required to be cultured in a sterile environment before the fermentation of the microorganisms is started, when a fermentation tank is put into a prepared culture medium, the culture medium is sterilized at high temperature and then cooled to the temperature required by the process, the prepared microorganism strains are transferred into the fermentation tank for inoculation, but after inoculation, the prepared microorganism strains are required to be transferred onto a turntable to drive the fermentation tank to shake so as to accelerate the mixing speed, the fermentation cannot be completed in one device, other bacteria are easy to contact in the taking process, the mixing effect is affected, and the process is time-consuming and labor-consuming.
Disclosure of Invention
Aiming at the defects and shortcomings of the prior art, the invention provides the solid fermentation liquid strain inoculation device which is reasonable in design and convenient to use, when the fermentation tank is cooled to a certain temperature, the fermentation tank is placed in the device, after the fermentation tank is cooled, the microorganism strain is added into the fermentation tank, the transfer is not needed, the fermentation tank is directly rocked, the mixing speed is increased, and the time and the labor are saved while other bacteria are not easy to contact.
In order to achieve the above purpose, the invention adopts the following technical scheme: the automatic feeding device comprises a box body, supporting feet, a storage box and a discharge pipe, wherein the supporting feet are fixed at four corners of the lower surface of the box body, the storage box is fixed on the upper side wall of the box body, the discharge pipe is inserted on the lower side wall of the storage box, and the lower end of the discharge pipe is suspended on the upper side of the inside of the box body after penetrating through the upper side wall of the box body; the automatic feeding device comprises a box body, a feeding mechanism, a fixing mechanism and a rotating mechanism, wherein the feeding mechanism is arranged on the upper side of the inside of the box body, the upper end of a telescopic pipe in the feeding mechanism is connected with the lower end of a discharge pipe, the fixing mechanism is arranged on the lower side of the feeding mechanism, the fixing mechanism is arranged on the middle side of the inside of the box body, the center of the lower surface of a placing plate in the fixing mechanism is fixedly connected with a rotating rod in the rotating mechanism, the lower end of the rotating rod is in screwed connection with the lower side wall of the box body through a bearing, the box body is of a front side open structure, a door body is arranged in the front side of the box body, the outer side wall of the door body is in sealed arrangement with the box body through a sealing strip, one side of the door body is in screwed connection with the front side wall of one side of the box body through a hinge, through holes are formed in the left side and the right side of the middle side of the door body, rubber gloves are arranged in the through holes, the outer periphery of the top ends of the rubber gloves are fixedly connected with the inner peripheral walls of the through holes, and the finger ends of the rubber gloves are suspended in the box body;
the feeding mechanism further comprises a spring, a fixing plate, a first electric push rod, a storage battery, a feeding pipe, a stop lever and a control switch, wherein the spring is fixed on the inner side wall of the upper side of the box body, the fixing plate is fixed at the lower end of the spring, the fixing plate is suspended in the box body, one side of the fixing plate is fixed on one side of the outer side wall of the feeding pipe, the stop lever is inserted in the feeding pipe, the other end of the stop lever penetrates through one side wall of the feeding pipe and then is fixedly connected with the first electric push rod, the first electric push rod is fixed on the lower surface of the fixing plate, the storage battery is fixed on the other side of the lower surface of the fixing plate, the storage battery is connected with the first electric push rod, the first electric push rod is connected with the control switch, and the control switch is fixed on the other side of the outer annular wall of the feeding pipe;
the fixing mechanism further comprises a sleeve, a plug rod, a limiting frame and a limiting plate, wherein the sleeve is fixed on the upper surface of the placing plate at equal angles, the plug rods are inserted into the sleeve and fixed through bolts, the upper ends of the plug rods penetrate through the upper ends of the sleeve and are fixedly connected with the lower surface of the limiting frame, the limiting frame is in a U-shaped arrangement, the limiting frame is sleeved on the outer edge of the limiting plate at equal angles, a plurality of limiting holes are formed in the limiting plate at equal angles, the limiting holes are sleeved on the bottleneck of the fermentation tank, and the fermentation tank is placed on the placing plate;
above-mentioned rotary mechanism still contains motor and universal wheel, and the motor is fixed on the lateral wall of box downside, and the motor is connected with external power source, and the output shaft of motor inserts and establishes in the lateral wall of box, and the output shaft of motor and the lower extreme fixed connection of bull stick, and the outside equiangle of bull stick upper end is equipped with the several universal wheel, and the connecting rod equiangular on the universal wheel is fixed on the lower surface of placing the board, and the downside activity of universal wheel sets up on the inside wall of box downside.
Further, the middle end of fermentation cylinder all overlap and be equipped with the packing ring, this packing ring is fixed on the lower surface of limiting plate, and the packing ring is the same centre of a circle setting with spacing hole, when fixing, keeps apart fermentation cylinder and limiting plate through the packing ring, reduces the harm.
Further, the left and right sides of the upper side of the discharge pipe are respectively provided with a guide plate, one side of each guide plate is fixed on the inner side wall of the lower side of the storage box, and after the other side of each guide plate is respectively inclined upwards, the guide plates are fixed on the inner side walls of the left and right sides of the storage box, and guide strains in the storage box through the guide plates.
Further, a side wall of the box body is inserted with an air inlet pipe, the outer end of the air inlet pipe is provided with a first check valve, the left side and the right side of the upper side wall of the box body are respectively inserted with an air outlet pipe, the upper end of the air outlet pipe is exposed out of the upper side of the box body, the outer end of the air outlet pipe is provided with a second check valve, when the box body is used, the fermentation tank is placed in the box body, high-temperature steam is connected through the air inlet pipe, the inside of the box body is disinfected through the high-temperature steam, and the air is discharged through the air outlet pipe after the disinfection.
Further, the upside of limiting plate hang and be equipped with the disinfection board, equiangular on this disinfection board is equipped with the circular hole of several, the circular hole sets up corresponding with the limiting hole, and the centre of a circle of circular hole is same perpendicular with the centre of a circle of limiting hole and sets up, the embedded heating collar that is equipped with in circular hole, this heating collar is connected with external power source, equiangular on the upper surface of disinfection board is fixed with the electric putter No. two, no. two electric putter is connected with external power source, no. two electric putter's upper end is fixed with the backup pad respectively, the backup pad is fixed respectively on the inner peripheral wall of box, when adding the bacterial in the fermentation cylinder, promote disinfection board downwardly through No. two electric putter for the heating collar is located the bottleneck department of fermentation cylinder, then starts the heating collar, clear away peripheral bacterium through the heating collar.
Further, the downside equiangular of disinfection board be equipped with the several stopper, this stopper sets up in the outside on fermentation cylinder top, the stopper is fixed respectively on the inner peripheral wall of box, the in-process of disinfection board downstream carries out spacingly to the disinfection board through the stopper.
The working principle of the invention is as follows: during use, a microbial strain is placed in a storage tank, then a door body is opened, a plug rod at the lower end of a limiting plate is taken out from a sleeve, a plurality of fermentation tanks are placed on a placing plate at equal angles, the limiting holes in the limiting plate are positioned and the limiting holes in the limiting plate are corresponding to each other, the limiting holes in the limiting plate are sleeved on the bottleneck at the top end of the fermentation tank, the plug rod is inserted into the sleeve until the lower surface of the limiting plate abuts against the bottle belly of the fermentation tank, a bolt on the sleeve is screwed down, the door body is closed, the high temperature on the fermentation tank can sterilize the interior of the tank, after the fermentation tank is cooled, a worker passes through a through hole in the door body respectively, and inserts into rubber gloves, and wears the rubber gloves, then a sealing plug on the fermentation tank is taken down, the other hand holds a conveying pipe, and one finger is placed on a control switch, then the conveying pipe is pulled to the upper side of the fermentation tank, a control switch is pressed down, the control switch is started, an electric push rod is driven to move the stop lever, the stop lever is pulled out of the conveying pipe is pulled out from the bottle belly, a motor is pulled down, a sealing plug is driven by the control switch is driven to rotate the inner side of the fermentation tank, the fermentation tank is further pulled down, the inner side is driven to rotate the fermentation tank is pulled down, the fermentation tank is further rotated, the inner side is pulled up, and the elastic plug is further is pulled up, and the fermentation tank is pulled down, and the inner side is pulled down, and the fermentation tank is turned down, and the inner is turned down, and the machine is turned down and the machine is turned down and is turned, and then the strain in the fermentation tank and the culture medium are mixed.
After the structure is adopted, the beneficial effects of the invention are as follows:
1. before the fermentation tank is cooled to a certain temperature, the fermentation tank is placed in the device, after the fermentation tank is cooled, the microbial strain is added into the fermentation tank, the fermentation tank is directly rocked without transferring, the mixing speed is increased, other bacteria are not easy to contact, and time and labor are saved;
2. the fermentation tank is fixed on the placing plate through the fixing mechanism, and the limiting plate is connected with the sleeve on the placing plate through the inserted link, so that the stability of the fermentation tank is improved while the removal and the fixation of the fermentation tank are not hindered;
3. the lower surface of the placing plate is fixed with a plurality of universal wheels at equal angles, so that the stability of the placing plate is improved while the placing plate is not prevented from rotating.
Drawings
Fig. 1 is a schematic structural view of the present invention.
Fig. 2 is a cross-sectional view A-A of fig. 1.
Fig. 3 is a top view of fig. 1.
Fig. 4 is a sectional view of B-B in fig. 3.
Fig. 5 is an enlarged view of a portion C in fig. 4.
Fig. 6 is an enlarged view of the portion D in fig. 4.
Fig. 7 is a schematic structural view of the sterilizing panel according to the present invention.
Fig. 8 is a schematic view of the bottom structure of the limiting plate in the present invention.
Reference numerals illustrate:
the device comprises a box body 1, supporting legs 2, a storage box 3, a discharge pipe 4, a feeding mechanism 5, a telescopic pipe 5-1, a spring 5-2, a fixed plate 5-3, an electric push rod 5-4, a storage battery 5-5, a feeding pipe 5-6, a stop lever 5-7, a control switch 5-8, a fixed mechanism 6, a placing plate 6-1, a sleeve 6-2, a plug rod 6-3, a limiting frame 6-4, a limiting plate 6-5, a limiting hole 6-6, a rotating mechanism 7, a rotating rod 7-1, a motor 7-2, a universal wheel 7-3, a door body 8, a through hole 9, rubber gloves 10, a fermentation tank 11, a gasket 12, a guide plate 13, an air inlet pipe 14, a one-way valve 15, an air outlet pipe 16, a one-way valve 17, a sterilizing plate 18, a circular hole 19, a heating ring 20, an electric push rod 21, a supporting plate 22 and a limiting block 23.
The specific embodiment is as follows:
the following description of the embodiments of the present invention will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present invention, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
As shown in fig. 1 to 8, the present embodiment adopts the following technical scheme: the device comprises a box body 1, supporting legs 2, a storage box 3 and a discharge pipe 4, wherein the supporting legs 2 are welded and fixed at four corners of the lower surface of the box body 1, the storage box 3 is fixed on the upper side wall of the box body 1 through bolts, the discharge pipe 4 is inserted and fixed on the lower side wall of the storage box 3, the lower end of the discharge pipe 4 passes through the upper side wall of the box body 1 and then is suspended on the upper side of the interior of the box body 1, guide plates 13 are arranged on the left side and the right side of the upper side of the discharge pipe 4, one side of each guide plate 13 is welded and fixed on the inner side wall of the lower side of the storage box 3, and after the other side of each guide plate 13 is respectively inclined upwards, the other side of each guide plate 13 is welded and fixed on the inner side walls of the left side and the right side of the storage box 3, so that strains are prevented from being stagnated on the two sides of the interior of the storage box 3; the automatic feeding device comprises a feeding mechanism 5, a fixing mechanism 6 and a rotating mechanism 7, wherein the feeding mechanism 5 is arranged on the upper side of the inside of a box body 1, the upper end of a telescopic pipe 5-1 in the feeding mechanism 5 is connected with the lower end of a discharge pipe 4, the feeding mechanism 5 further comprises a spring 5-2, a fixing plate 5-3, a first electric push rod 5-4, a storage battery 5-5, a feed pipe 5-6, a stop rod 5-7 and a control switch 5-8, the spring 5-2 is fixedly welded on the inner side wall of the upper side of the box body 1, the fixing plate 5-3 is fixedly welded at the lower end of the spring 5-2, the fixing plate 5-3 is suspended in the inside of the box body 1, the left side of the fixing plate 5-3 is fixedly welded on the right side of the outer side wall of the feed pipe 5-6, the stop rod 5-7 is movably inserted in the inside of the feed pipe 5-6, the right end of the stop rod 5-7 passes through the right side wall of the feed pipe 5-6 and is fixedly connected with the first electric push rod 5-4 through a bolt, the first electric push rod 5-4 is fixedly connected with the lower surface of the fixing plate 5-3 through a bolt, the electric push rod 5-3 is fixedly connected with the right side of the electric push rod 5-4 through the bolt, the right side of the electric push rod 5-6 is fixedly connected with the right side of the control switch 5-4 through the right side of the electric push rod 5-6 through the right side 5-4 through the control switch, and the control switch is fixedly connected with the right side 5-4 through the right side of the electric push rod 5-4;
the lower side of the feeding mechanism 5 is provided with a fixing mechanism 6, the fixing mechanism 6 is arranged at the middle side of the inside of the box body 1, the fixing mechanism 6 is composed of a placing plate 6-1, a sleeve 6-2, an inserting rod 6-3, a limiting frame 6-4 and a limiting plate 6-5, the sleeve 6-2 is welded and fixed on the upper surface of the placing plate 6-1 at equal angles, the inserting rods 6-3 are inserted into the sleeve 6-2, the inserting rods 6-3 are fixed through bolts, the upper ends of the inserting rods 6-3 penetrate through the upper ends of the sleeve 6-2 and are welded and fixed with the lower surface of the limiting frame 6-4, the limiting frame 6-4 is U-shaped, the limiting frame 6-4 is sleeved on the outer edge of the limiting plate 6-5 at equal angles, a plurality of limiting holes 6-6 are formed in the limiting plate 6 at equal angles, the limiting holes 6-6 are sleeved on the fermentation tank 11, the fermentation tank 11 is placed on the placing plate 6-1, the middle ends of the fermentation tank 11 are sleeved with gaskets 12, the gaskets 12 are adhered and fixed on the lower surface of the limiting plate 6-5, the gaskets 12 and the same in shape as the limiting plate 6-5, and the limiting holes 6-5 are arranged at equal angles, so that the damage to the fermentation tank can be prevented from being caused to the lower than the limit tank 6-5;
the center of the lower surface of the placing plate 6-1 in the fixing mechanism 6 is welded and fixed with a rotating rod 7-1 in the rotating mechanism 7, the lower end of the rotating rod 7-1 is screwed and connected with the lower side wall of the box body 1 through a bearing, the bearing is embedded in the lower side wall of the box body 1, the outer ring of the bearing is welded and fixed with the inner side wall of the lower side of the box body 1, the inner ring of the bearing is welded and fixed with the rotating rod 7-1, the rotating mechanism 7 also comprises a motor 7-2 and universal wheels 7-3, the motor 7-2 is fixed on the outer side wall of the lower side of the box body 1 through bolts, the model of the motor 7-2 is 50KTYZ, the motor 7-2 is connected with an external power supply, the output shaft of the motor 7-2 is inserted in the lower side wall of the box body 1, the output shaft of the motor 7-2 is welded and fixed with the lower end of the rotating rod 7-1, the outer side of the upper end of the rotating rod 7-1 is provided with a plurality of universal wheels 7-3, the connecting rod on the universal wheel 7-3 is welded and fixed on the lower surface of the placing plate 6-1 at equal angles, the lower side of the universal wheel 7-3 is movably arranged on the inner side wall of the lower side of the box body 1, the box body 1 is of a front-side open structure, a door body 8 is arranged in the front side of the box body 1, the outer side wall of the door body 8 is arranged in a sealing way with the box body 1 through a sealing strip, the right side of the door body 8 is screwed with the front side wall of the right side of the box body 1 through a hinge, the right side of the hinge is fixedly connected with the front side wall of the right side wall of the box body 1 through a bolt, the left side of the hinge is fixedly connected with the front side wall of the right side of the box body 8 through a cross lock, the cross lock is embedded in the left side of the door body 8, a lock cylinder of the cross lock is embedded in the left side wall of the box body 1 after penetrating through the left side wall of the door body 8, the left and right sides of the middle side of the door body 8 are provided with through holes 9, rubber gloves 10 are arranged in the through holes 9, the outer periphery of the top ends of the rubber gloves 10 are fixedly adhered to the inner peripheral wall of the through holes 9, the finger ends of the rubber gloves 10 are suspended in the box body 1, an air inlet pipe 14 is inserted and welded on the left side wall of the box body 1, a first check valve 15 is arranged at the outer end of the air inlet pipe 14, air outlet pipes 16 are inserted on the left and right sides of the upper side wall of the box body 1, the upper ends of the air outlet pipes 16 are exposed on the upper side of the box body 1, and second check valves 17 are arranged at the outer ends of the air outlet pipes 16, so that the interior of the box body 1 can be disinfected, and bacteria in the box body 1 can be reduced;
the upper side of limiting plate 6-5 hangs and is equipped with disinfection board 18, the equiangular round hole 19 of having seted up on this disinfection board 18, round hole 19 and limiting hole 6-6 correspond the setting, and the centre of a circle of round hole 19 and limiting hole 6-6's centre of a circle are the same perpendicular setting, the embedded heating collar 20 that is equipped with of round hole 19, this heating collar 20 is connected with external power source, equiangular electric putter 21 of a plurality of No. two on the upper surface of disinfection board 18 through the bolt fastening, no. two electric putter 21 is connected with external power source, no. two electric putter 21's upper end is fixed with backup pad 22 through the bolt fastening respectively, backup pad 22 welded fastening respectively on the interior perisporium of box 1, can conveniently eliminate the bacterium of fermentation cylinder 11 periphery, the downside of disinfection board 18 is equipped with a plurality of stopper 23, this stopper 23 sets up in the outside on fermentation cylinder 11 top, stopper 23 welded fastening respectively on the interior perisporium of box 1, can prevent disinfection board 18 cover to the top of fermentation cylinder 11, cause the injury to fermentation cylinder 11.
The working principle of the specific embodiment is as follows: when in use, microorganism strains are placed in the storage box 3, then the door body 8 is opened, the inserted link 6-3 at the lower end of the limiting plate 6-5 is taken out from the sleeve 6-2, then a plurality of fermentation tanks 11 are placed on the placing plate 6-1 at equal angles, the positions and the number of the limiting holes 6-6 on the limiting plates 6-5 are corresponding respectively, the limiting holes 6-6 on the limiting plates 6-5 are sleeved on the bottleneck at the top end of the fermentation tanks 11, the inserted link 6-3 is inserted into the sleeve 6-2 respectively until the lower surface of the limiting plates 6-5 is abutted on the bottle belly of the fermentation tanks 11, the bolts on the sleeve 6-2 are screwed, the door body 8 is closed, the high temperature on the fermentation tanks 11 can disinfect the inside of the box 1, after the fermentation tanks 11 are cooled, the staff passes through the through holes 9 on the door body 8 respectively, inserted into the rubber glove 10 and worn, then the sterilizing plate 18 is pushed downwards by the second electric push rod 21, so that the heating ring 20 is positioned at the bottleneck of the fermentation tank 11, then the heating ring 20 is started, peripheral bacteria are removed by the heating ring 20, the sealing plug on the fermentation tank 11 is removed by one hand, the feeding pipe 5-6 is held by the other hand, one finger is placed on the control switch 5-8, then the feeding pipe 5-6 is pulled to the upper side of the fermentation tank 11, the control switch 5-8 is pressed, the control switch 5-8 starts the first electric push rod 5-4, the stop lever 5-7 is driven to move outwards by the first electric push rod 5-4, after the stop lever 5-7 is pulled out from the feeding pipe 5-6, bacteria in the telescopic pipe 5-1 drop downwards, after a proper amount of bacteria are dropped, the control switch 5-8 is released, the control switch 5-8 closes the first electric push rod 5-4, the first electric push rod 5-4 drives the stop rod 5-7 to move inwards, the stop rod 5-7 is inserted into the feed pipe 5-6, strains on the upper side of the telescopic pipe 5-1 are blocked again, then sealing plugs on the fermentation tank 11 are covered again, the operation of the next fermentation tank 11 is continued, and the like until all the fermentation tanks 11 are filled with strains, the feed pipe 5-6 is released again, the feed pipe 5-6 is pulled upwards due to the elasticity of the springs 5-2, hands of a worker are pulled out of the rubber glove 10, the motor 7-2 is started again, the motor 7-2 drives the rotary rod 7-1 to rotate, the rotary rod 7-1 drives the placing plate 6-1 to rotate, and the placing plate 6-1 drives the fermentation tank 11 to rotate, so that the strains and the culture medium in the fermentation tank 11 are mixed.
After adopting above-mentioned structure, this embodiment's beneficial effect is as follows:
1. before the fermentation tank 11 is cooled to a certain temperature, the fermentation tank 11 is placed in the device, after the cooling is completed, the microorganism strain is added into the fermentation tank 11, the fermentation tank 11 is directly rocked without transferring, the mixing speed is increased, and the time and the labor are saved while other bacteria are not easy to contact;
2. the fermentation tank 11 is fixed on the placing plate 6-1 through the fixing mechanism 6, and the limiting plate 6-5 is connected with the sleeve 6-2 on the placing plate 6-1 through the inserted rod 6-3, so that the stability of the fermentation tank 11 is improved while the removal and the fixation of the fermentation tank are not hindered;
3. a plurality of universal wheels 7-3 are fixed on the lower surface of the placing plate 6-1 at equal angles, so that the stability of the placing plate 6-1 is improved while the rotation of the placing plate 6-1 is not hindered;
4. the upper side of the limiting plate 6-5 is provided with a sterilizing plate 18, and the sterilizing plate 18 can move to the upper side of the fermentation tank 11 when strains are added, so that the periphery of the strains is sterilized in the process of adding the strains, and the strains are prevented from being infected by other bacteria.
Although the present invention has been described with reference to the foregoing embodiments, it will be apparent to those skilled in the art that modifications may be made to the embodiments described, or equivalents may be substituted for elements thereof, and any modifications, equivalents, improvements and changes may be made without departing from the spirit and principles of the present invention.
Claims (3)
1. The solid fermentation liquid strain inoculation device is characterized by comprising a box body (1), wherein supporting legs (2) are fixed at four corners of the lower surface of the box body (1), a storage box (3) is fixed on the upper side wall of the box body (1), a discharge pipe (4) is inserted into the lower side wall of the storage box (3), and the lower end of the discharge pipe (4) penetrates through the upper side wall of the box body (1) and then is suspended at the upper side inside the box body (1); the box body (1) is of a front side open structure, a door body (8) is arranged in the front side of the box body (1), the outer side wall of the door body (8) is in sealing arrangement with the box body (1) through a sealing strip, one side of the door body (8) is connected with the front side wall of one side of the box body (1) in a screwed mode through a hinge, the other side of the door body (8) is fixedly connected with the front side wall of the other side of the box body (1) through a cross lock, through holes (9) are formed in the left side and the right side of the middle side of the door body (8), rubber gloves (10) are arranged in the through holes (9), the outer periphery of the top ends of the rubber gloves (10) are fixedly connected with the inner peripheral wall of the through holes (9), and finger ends of the rubber gloves (10) are suspended in the box body (1); an air inlet pipe (14) is inserted into one side wall of the box body (1), a first check valve (15) is arranged at the outer end of the air inlet pipe (14), air outlet pipes (16) are respectively inserted into the left side and the right side of the upper side wall of the box body (1), the upper ends of the air outlet pipes (16) are exposed out of the upper side of the box body (1), a second check valve (17) is respectively arranged at the outer ends of the air outlet pipes (16), the air inlet pipe (14) is connected with high-temperature steam, and the high-temperature steam is used for disinfecting the inside of the box body (1) and is discharged through the air outlet pipes (16) after disinfection;
the device also comprises a feeding mechanism (5), a fixing mechanism (6) and a rotating mechanism (7);
the feeding mechanism (5) is arranged at the upper side of the inside of the box body (1); the feeding mechanism (5) comprises a telescopic pipe (5-1), a spring (5-2), a fixed plate (5-3), a first electric push rod (5-4), a storage battery (5-5), a feeding pipe (5-6), a stop lever (5-7) and a control switch (5-8); the upper end of the telescopic pipe (5-1) is connected with the lower end of the discharge pipe (4), guide plates (13) are arranged on the left side and the right side of the upper side of the discharge pipe (4), one side of each guide plate (13) is fixed on the inner side wall of the lower side of the storage box (3), the other side of each guide plate (13) is fixed on the inner side walls of the left side and the right side of the storage box (3) after being inclined upwards respectively, and strains in the storage box (3) are guided through the guide plates (13); the spring (5-2) is fixed on the inner side wall of the upper side of the box body (1), the lower end of the spring (5-2) is fixedly provided with a fixing plate (5-3), the fixing plate (5-3) is suspended in the box body (1), one side of the fixing plate (5-3) is fixed on one side of the outer side wall of the feeding pipe (5-6), a stop lever (5-7) is inserted in the feeding pipe (5-6), the other end of the stop lever (5-7) penetrates through one side wall of the feeding pipe (5-6) and is fixedly connected with a first electric push rod (5-4), the first electric push rod (5-4) is fixed on the lower surface of the fixing plate (5-3), the other side of the lower surface of the fixing plate (5-3) is fixedly provided with a storage battery (5-5), the storage battery (5-5) is connected with the first electric push rod (5-4), the first electric push rod (5-4) is connected with a control switch (5-8), and the control switch (5-8) is fixed on the other side of the outer ring wall of the feeding pipe (5-6);
the fixing mechanism (6) is arranged at the lower side of the feeding mechanism (5), and the fixing mechanism (6) is arranged at the middle side of the inside of the box body (1); the fixing mechanism (6) comprises a placing plate (6-1), a sleeve (6-2), an inserting rod (6-3), a limiting frame (6-4) and a limiting plate (6-5); the novel fermentation tank is characterized in that the sleeves (6-2) are fixed on the upper surface of the placement plate (6-1) at equal angles, the inserted rods (6-3) are inserted into the sleeves (6-2) and fixed through bolts, the upper ends of the inserted rods (6-3) penetrate through the upper ends of the sleeves (6-2) and are fixedly connected with the lower surface of the limiting frames (6-4), the limiting frames (6-4) are arranged in a U shape, the limiting frames (6-4) are sleeved on the outer edges of the limiting plates (6-5) at equal angles, a plurality of limiting holes (6-6) are formed in the limiting plates (6-5) at equal angles, the limiting holes (6-6) are sleeved on the bottleneck of the fermentation tank (11), and the fermentation tank (11) is placed on the placement plate (6-1); the middle ends of the fermentation tanks (11) are respectively sleeved with a gasket (12), the gaskets (12) are fixed on the lower surfaces of the limiting plates (6-5), the gaskets (12) and the limiting holes (6-6) are arranged at the same circle center, and the fermentation tanks (11) and the limiting plates (6-5) are isolated by the gaskets (12) during fixation so as to reduce damage;
the rotating mechanism (7) comprises a rotating rod (7-1), a motor (7-2) and a universal wheel (7-3); the lower end of the rotating rod (7-1) is screwed with the lower side wall of the box body (1) through a bearing, and the center of the lower surface of the placing plate (6-1) in the fixing mechanism (6) is fixedly connected with the rotating rod (7-1) in the rotating mechanism (7); the motor (7-2) is fixed on the outer side wall of the lower side of the box body (1), the motor (7-2) is connected with an external power supply, an output shaft of the motor (7-2) is inserted into the lower side wall of the box body (1), and the output shaft of the motor (7-2) is fixedly connected with the lower end of the rotating rod (7-1); the outer side of the upper end of the rotating rod (7-1) is provided with a plurality of universal wheels (7-3) at equal angles, connecting rods on the universal wheels (7-3) are fixed on the lower surface of the placing plate (6-1) at equal angles, and the lower side of the universal wheels (7-3) is movably arranged on the inner side wall of the lower side of the box body (1);
the using process of the solid fermentation liquid strain inoculation device comprises the following steps: placing microorganism strains in a storage box (3), opening a door body (8), taking out a plug rod (6-3) at the lower end of a limiting plate (6-5) from a sleeve (6-2), then placing a plurality of fermentation tanks (11) on the storage box (6-1) at equal angles, wherein the limiting holes (6-6) on the limiting plate (6-5) are positioned and the limiting holes are corresponding to each other, sleeving the limiting holes (6-6) on the limiting plate (6-5) on the bottleneck at the top end of the fermentation tank (11), respectively inserting the plug rod (6-3) into the sleeve (6-2) until the lower surface of the limiting plate (6-5) is abutted against the bottle belly of the fermentation tank (11), screwing down a bolt on the sleeve (6-2), closing the door body (8), sterilizing the inside of the box (1) by utilizing high-temperature steam, respectively inserting a worker into a rubber glove (10) through a through hole (9) on the door body (8) after the fermentation tank (11) is cooled, respectively holding the fermentation tank (11) on one hand, holding the other hand (5) on the other hand (5), holding the other hand (5) on the other hand (5) and holding the other hand (5) on the hand (5), the control switch (5-8) starts the first electric push rod (5-4), the first electric push rod (5-4) drives the stop lever (5-7) to move outwards, the stop lever (5-7) is pulled out of the feeding pipe (5-6), the strains in the telescopic pipe (5-1) drop downwards, after a proper number of strains are dropped, the control switch (5-8) is released, the control switch (5-8) is closed, the first electric push rod (5-4) drives the stop lever (5-7) to move inwards, the stop lever (5-7) is inserted into the feeding pipe (5-6) to stop the strains on the upper side of the telescopic pipe (5-1) again, a sealing plug on the fermentation tank (11) is covered again, the operation of the next fermentation tank (11) is continued, and the like until all the fermentation tanks (11) are filled with the strains; then loosening the feeding pipe (5-6), pulling up the feeding pipe (5-6) by the elastic force of the spring (5-2), pulling out hands of a worker from the rubber glove (10), starting the motor (7-2), driving the rotating rod (7-1) to rotate by the motor (7-2), driving the placing plate (6-1) to rotate by the rotating rod (7-1), and driving the fermentation tank (11) to rotate by the placing plate (6-1), so that strains and culture mediums in the fermentation tank (11) are mixed.
2. The solid state fermentation liquid seed inoculant device of claim 1, wherein: the upper side of the limiting plate (6-5) is suspended with a sterilizing plate (18), a plurality of round holes (19) are formed in the sterilizing plate (18) at equal angles, the round holes (19) are arranged corresponding to the limiting holes (6-6), the circle centers of the round holes (19) and the circle centers of the limiting holes (6-6) are arranged in the same vertical plane, a heating ring (20) is embedded in the round holes (19), the heating ring (20) is connected with an external power supply, a plurality of second electric push rods (21) are fixed on the upper surface of the sterilizing plate (18) at equal angles, the second electric push rods (21) are connected with the external power supply, supporting plates (22) are respectively fixed at the upper ends of the second electric push rods (21), and the supporting plates (22) are respectively fixed on the inner peripheral wall of the box body (1); when strains are added into the fermentation tank (11), the sterilizing plate (18) is pushed downwards through the second electric push rod (21) to enable the heating ring (20) to be positioned at the bottle mouth of the fermentation tank (11), then the heating ring (20) is started, and bacteria on the periphery of the bottle mouth are removed through the heating ring (20).
3. The solid state fermentation liquid seed inoculum apparatus according to claim 2, wherein: a plurality of limiting blocks (23) are arranged on the lower side of the disinfection plate (18) at equal angles, the limiting blocks (23) are arranged on the outer side of the top end of the fermentation tank (11), and the limiting blocks (23) are respectively fixed on the inner peripheral wall of the box body (1); and the sterilizing plate (18) is limited by a limiting block (23) in the process of downwards moving the sterilizing plate (18).
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CN111925920A (en) * | 2020-08-14 | 2020-11-13 | 常州惠农生物科技有限公司 | Culture bottle for anaerobic bacteria fermentation |
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