CN111849731A - A kind of gene amplification tube and detection cartridge - Google Patents

Abstract

The invention provides a gene amplification tube and a detection card box, wherein the gene amplification tube comprises a tube body and a cover, the top of the tube body is provided with a tube opening, the bottom of the tube body is sealed, the cover seals the tube opening at the top of the tube body, the tube body comprises a side tube wall and a bottom tube wall, the side tube wall comprises a cylindrical side tube wall and a conical side tube wall positioned below the cylindrical side tube wall, a concave area which is concave towards the inside of the tube body is arranged on the bottom tube wall, and the thickness of the bottom tube wall in the concave area is smaller than that of the side tube wall. By adopting the gene amplification tube, the bottom of the gene amplification tube is provided with the recess, and the wall thickness of the bottom is thinner, so that the gene amplification tube can be effectively prevented from being accidentally broken in the using process compared with the prior art in which the recess and the nick outside the tube body are protruded.

Description

A kind of gene amplification tube and detection cartridge

technical field

The invention relates to the field of detection, in particular to a gene amplification tube and a detection cartridge.

Background technique

Nucleic acid diagnosis is one of the most dynamic segments in the future IVD (in vitro diagnostic) industry. The increase in the prevention and control of infectious diseases in my country, the promotion of nucleic acid detection by blood screening, and the development of individualized medicine are the main driving forces for the development of domestic nucleic acid diagnosis. Driven by these factors, the future growth rate of domestic nucleic acid diagnosis will be between 25-30%, significantly exceeding the average growth rate of the domestic IVD industry. On the one hand, nucleic acid diagnosis benefits large medical centers and realizes early, rapid, specific, and high-throughput detection of pathogens and genetic diseases.

POCT (point-of-care testing), which means "point-of-care testing" in Chinese, is an emerging industry segment of in vitro diagnosis (IVD). , a new class of methods to get test results quickly. The main criteria of POCT are that no fixed testing site is required, reagents and instruments are portable, and can be operated in a timely manner. POCT undertakes the functions of a laboratory but does not require traditional hospital laboratory equipment, and can serve patients 24 hours a day regardless of time and location.

However, these nucleic acid amplification methods have the problem that amplification products are prone to cross-contamination, and false positive signals generated by product contamination can cause erroneous interpretation of detection results. Cross-contamination between samples is often seen during target nucleic acid amplification operations. The contamination may come from known or unknown positive substances introduced during the processing of negative samples, which may cause false positive reactions through air pollution or aerosols.

In the prior art, a series of methods have been developed to prevent cross-contamination of amplification products. For example, reference 1 (CN105199940A) discloses a pollution-proof portable genetic detection method and device, through which the amplification products can be detected. After the PCR tube is put into the device for sealing, the PCR tube is punctured to realize the detection. Prevent nucleic acid amplification product contamination and avoid false positives.

SUMMARY OF THE INVENTION

In order to facilitate puncturing the bottom of the gene amplification tube, the fully enclosed detection cartridge in the prior art generally has a notch at the bottom, which protrudes from the outside of the gene amplification tube, compared with the traditional gene amplification tube. However, before the gene amplification tube is inserted into the detection cartridge, it may break along the incision when moving or performing the amplification reaction, causing the solution of the sample to be tested to leak, causing great harm.

The purpose of the present invention is to solve the problem that the solution tube of the sample to be tested is easily damaged in the prior art. In order to solve the above problems, the present invention discloses a gene amplification tube and detection cartridge with a brand-new structure. Through the gene amplification tube and detection cartridge, the possibility of leakage of the sample to be tested is greatly reduced, and the safety of even detection is ensured. sex.

The invention discloses a gene amplification tube, which includes a tube body and a cover. The top of the tube body is provided with a tube opening and the bottom is sealed. The cover seals the top tube opening of the tube body. The tube body includes a side tube wall and a bottom tube wall. The pipe wall includes a cylindrical side pipe wall and a conical side pipe wall located below the cylindrical side pipe wall. The bottom pipe wall is provided with a concave area recessed into the interior of the pipe body, and the thickness of the bottom pipe wall in the concave area is smaller than that of the side pipe. wall thickness.

By adopting the technical solution of the present invention, by setting a depression at the bottom of the gene amplification tube and setting the wall thickness of the bottom to be thinner, compared with the notch on the outside of the tube in the prior art, it can be effectively avoided. The gene amplification tube accidentally ruptured during use.

According to another specific embodiment of the present invention, the thickness of the conical side pipe wall is smaller than that of the cylindrical side pipe wall, and the thickness of the bottom pipe wall in the recessed area is smaller than the thickness of the conical side pipe wall.

According to another specific embodiment of the present invention, the thickness of the conical side wall is less than 0.3 mm.

According to another specific embodiment of the present invention, the thickness of the bottom tube wall in the recessed area is less than 0.3 mm.

According to another specific embodiment of the present invention, the thickness of the bottom pipe wall in the recessed area is 0.1-0.2 mm.

According to another specific embodiment of the present invention, the present invention also discloses a detection cartridge, comprising an outer casing and any one of the aforementioned gene amplification tubes, the outer casing is provided with a accommodating cavity, and the upper surface of the outer casing is provided with an intubation port , the accommodating cavity below the intubation port is provided with a detection jam and a tube-breaking part from bottom to top. After the gene amplification tube is inserted into the intubation port, the accommodating cavity is sealed, and the tube-breaking part pierces the gene amplification tube, The nucleic acid solution flows into the accommodating chamber.

According to another specific embodiment of the present invention, a nucleic acid removal part is further provided in the accommodating cavity, and the nucleic acid removal part can be opened to allow the destruction liquid to flow into the accommodating cavity.

Description of drawings

Below in conjunction with the accompanying drawings and specific embodiments, the present invention will be described in further detail:

1 is a cross-sectional view of a gene amplification tube provided by the present invention;

Fig. 2 is the structural representation of the gene amplification tube provided by the present invention;

3 is a cross-sectional view of the gene amplification tube provided by the present invention and the detection cartridge in a matched state;

4 is a cross-sectional view of the gene amplification tube and the detection cartridge provided by the present invention in another state of cooperation;

5 is a schematic structural diagram of the gene amplification tube and the detection cassette provided by the present invention in a state of cooperation;

6 is a schematic diagram of the explosion of the gene amplification tube and the detection cassette provided by the present invention;

Fig. 7 is the structural representation of the broken pipe part provided by the present invention;

FIG. 8 is a schematic structural diagram of the broken pipe part provided by the present invention.

Reference number:

Gene Amplification Tube 100

side wall 110

first protrusion 111

Bottom tube wall 120

Recessed area 121

cover 130

Nucleic acid solution storage chamber 140

seal ring 150

temperature indicator 160

Shell 200

accommodating cavity 210

Nucleic acid removal section 220

Liquid Seals 230

Detect jams 240

Pipe Breaker 250

Positioning Section 251

Fourth limit part 2511

The third protrusion 2512

Pierced 252

fluid channel 253

Intubation port 260

first groove 261

The third limit part 262

Test result observation area 270

Elastic press down structure 280

Detailed ways

The embodiments of the present invention are described below by specific embodiments, and those skilled in the art can easily understand other advantages and effects of the present invention from the contents disclosed in this specification. Although the description of the invention will be presented in conjunction with the preferred embodiment, this does not mean that the features of the invention are limited to this embodiment. On the contrary, the purpose of introducing the invention in conjunction with the embodiments is to cover other options or modifications that may be extended based on the claims of the invention. The following description will contain numerous specific details in order to provide a thorough understanding of the present invention. The invention may also be practiced without these details. Furthermore, some specific details will be omitted from the description in order to avoid obscuring or obscuring the gist of the present invention. It should be noted that the embodiments of the present invention and the features of the embodiments may be combined with each other under the condition of no conflict.

In the description of this embodiment, it should be noted that the orientations or positional relationships indicated by the terms "upper", "lower", "inside", "bottom", etc. are based on the orientations or positional relationships shown in the drawings, or are The orientation or positional relationship that the product of the invention is usually placed in use is only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, Therefore, it should not be construed as a limitation of the present invention.

The terms "first", "second", "third", etc. are only used to differentiate the description and should not be construed as indicating or implying relative importance.

In the description of this embodiment, it should also be noted that, unless otherwise expressly specified and limited, the terms "arranged", "connected" and "connected" should be understood in a broad sense, for example, it may be a fixed connection or a Removable connection, or integral connection; can be mechanical connection, can also be electrical connection; can be directly connected, can also be indirectly connected through an intermediate medium, can be internal communication between two components. For those of ordinary skill in the art, the specific meanings of the above terms in this embodiment can be understood in specific situations.

In order to make the objectives, technical solutions and advantages of the present invention clearer, the embodiments of the present invention will be further described in detail below with reference to the accompanying drawings.

As shown in FIG. 1 and FIG. 2, the present invention discloses a gene amplification tube 100, which includes a tube body and a cover 130, the top of the tube body is provided with a tube opening and the bottom is sealed, and the cover 130 seals the top tube opening of the tube body, The pipe body includes a side pipe wall 110 and a bottom pipe wall 120. The side pipe wall 110 includes a cylindrical side pipe wall and a conical side pipe wall located below the cylindrical side pipe wall. The bottom pipe wall 120 is provided with a depression toward the inside of the pipe body. In the concave area 121, the thickness of the bottom pipe wall 120 in the concave area 121 is smaller than the thickness of the side pipe wall 110.

By adopting the technical solution of the present invention, by providing a depression at the bottom of the gene amplification tube 100 and setting the wall thickness of the bottom to be thinner, compared with the protruding notch outside the tube in the prior art, it can effectively Avoid accidental rupture of the gene amplification tube 100 during use.

In addition, the lower part of the gene amplification tube 100 is set as a conical tube body, which can effectively increase the heat exchange area of the reaction system in the gene amplification tube 100, and ensure that the amplification reaction proceeds quickly and uniformly.

According to another specific embodiment of the present invention, in order to further reduce the waiting time for heating, the thickness of the conical side pipe wall is preferably smaller than the thickness of the cylindrical side pipe wall.

Further, because the thickness of the conical side tube wall is small, in order to avoid the deformation of the bottom of the gene amplification tube 100 to the inside of the tube body when the detection cartridge is inserted, so that the broken tube 250 cannot pierce the gene amplification tube smoothly. Therefore, the thickness of the bottom tube wall in the concave area 121 at the bottom of the gene amplification tube 100 is smaller than the thickness of the tapered side tube wall.

Further, in order to increase the heating efficiency of the gene amplification tube 100, the thickness of the tapered side tube wall and the bottom tube wall in the concave area should be less than 0.3 mm. More preferably, the thickness of the bottom tube wall in the recessed area is 0.1-0.2 mm.

According to another specific embodiment of the present invention, the gene amplification tube 100 in the present invention is a specially-made PCR tube, and the side wall 110 of the gene amplification tube 100 can be composed of a series of materials, which preferably have good thermal conductivity and high strength , Made of materials with good fluidity, such as metals, alloys, thermally conductive plastics and organic composite materials, the height is 1-3cm, preferably 2cm, and the general shape can be similar to ordinary PCR tubes, but there are differences.

According to another specific embodiment of the present invention, the gene amplification tube 100 of the present invention is provided with a temperature indicating part 160, and the temperature indicating part 160 may be a reversible temperature test paper. A suitable reversible temperature test paper can be selected according to the temperature of the nucleic acid amplification reaction. For example, when the nucleic acid amplification reaction temperature is 38 degrees, you can choose THERMAX reversible temperature test paper of 0 to 50 °C; if the nucleic acid amplification reaction requires 63 degrees, you can choose THERMAX reversible temperature test paper of 50 to 100 °C . In addition, special test strips can also be customized to suppliers according to the actual situation.

According to another specific embodiment of the present invention, the surface of the gene amplification tube 100 is coated with at least two kinds of reversible thermochromic materials. The discoloration temperature of the thermochromic material can be set according to actual needs, and the specific reversible thermochromic material can be a commercially available product. For a certain amplification reaction, if the reaction temperature needs to be between the first temperature T1 and the second temperature T2, two kinds of temperature-sensitive color-changing materials can be coated on the surface of the gene amplification tube 100, and the first temperature-sensitive color-changing material can be selected. The discoloration temperature of the material is the first temperature T1, and the discoloration temperature of the second thermochromic material is the second temperature T2. In this way, during the amplification reaction, when the first temperature-sensitive material changes color but the second temperature-sensitive material does not change color, it indicates that the temperature is suitable for the amplification reaction in the gene amplification tube 100 to be performed. In this way, the gene amplification tube 100 can be directly put into the thermos, and the amount of hot and cold water can be adjusted to control the temperature of the water in the thermos, so that the amplification reaction can be maintained. No constant temperature equipment is required, and the samples can be tested anytime, anywhere. detection.

For example, if the optimal reaction temperature is around 38 degrees, two temperature-sensitive coatings can be used, which are greater than 38 and less than 38 degrees, preferably 37 and 39 degrees. If the optimal reaction temperature is 63 degrees, then the temperature The temperature-sensitive paint can be selected as 62 and 64, and the shape of the temperature-sensitive paint can be arbitrary, but it is preferably an Arabic numeral corresponding to the temperature, such as a temperature-sensitive material with a color change of 38 degrees, which is displayed as "38". In this way, the temperature of the gene amplification tube 100 can be more directly reflected.

Further, if the gene amplification tube 100 is used in various amplification reaction systems with different temperatures, various temperature-sensitive color-changing materials can be provided.

According to another specific embodiment of the present invention, the general gene amplification tube 100 has an open cap structure, that is, the cap 130 is an openable cap, and the sample to be amplified and the reagents related to the amplification reaction system are placed in the After being put into the gene amplification tube 100, cover the lid to achieve sealing. In addition, the upper part of the gene amplification tube 100 can also be directly a closed structure, that is, the lid 130 and the side tube wall 110 are fixedly connected, or even directly integrally formed and cannot be opened. When in use, a syringe with a fine needle pierces the lid 130 of the gene amplification tube 100, injects the reaction system, and then seals the breach with a parafilm or a wax drop with a higher melting point, so as to better realize the function of the gene amplification tube 100. seal.

According to another specific embodiment of the present invention, one or more first protrusions 111 are provided on the outer surface of the side pipe wall 110 , if there are multiple first protrusions 111 , the multiple first protrusions 111 may be located at On the same cross section of the side pipe wall 110, at least part of the side pipe wall 110 is a cylindrical side pipe wall, the outer surface of the cylindrical side pipe wall is provided with an external thread (not shown in the figure), and the external thread is located on the first convex surface. below the section 111.

The structure of the first convex portion 111 is not particularly limited, as long as it can fit the first groove 261 to limit the position of the gene amplification tube 100 relative to the first channel in the axial direction, and the first convex portion 111 can It is a convex point, it can also be a convex strip extending in the circumferential direction, or a complete circular convex ring. From the perspective of more effective and more stable restriction of the position of the gene amplification tube 100 in the axial direction, preferably the first convex portion 111 is a complete circular convex ring, or a plurality of them are uniform along the circumferential direction of the side tube wall 110 Distributed bumps or ridges.

According to another specific embodiment of the present invention, as shown in FIG. 1 and FIG. 2 , the first convex portion 111 on the side wall 110 of the gene amplification tube 100 is a circular convex ring, and the gene amplification portion under the first convex portion 111 is a circular convex ring. The side pipe wall 110 of the extension pipe 100 is a cylindrical side pipe wall, and the cylindrical side pipe wall is provided with an external thread matched with the internal thread of the positioning part 251 of the broken pipe part 250 .

Further, as shown in FIG. 3 to FIG. 6 , a detection cartridge is also disclosed in the present invention, which includes a housing 200 and any one of the aforementioned gene amplification tubes 100 . The housing 200 is provided with a accommodating cavity 210 . The upper surface is provided with an intubation port 260, and the accommodating cavity 210 below the intubation port 260 is provided with a detection jam 240 and a tube-breaking portion 250 in sequence from bottom to top, and the intubation port 260 is set for gene amplification. The tube 100 is inserted, and after the gene amplification tube 100 is inserted into the intubation port 260 , the accommodating cavity 210 is sealed, the broken tube 250 pierces the bottom tube wall 130 of the gene amplification tube 100 , and the nucleic acid solution flows into the accommodating cavity 210 .

In some poor or backward areas, the gene amplification tube 100 provided by the present invention is used to complete the nucleic acid amplification reaction, and then the gene amplification tube 100 can be inserted into the intubation port 260 to quickly complete the nucleic acid detection, and Since the gene amplification tube 100 and the intubation port 260 are sealed, it can effectively prevent the sample from volatilizing into the air and polluting the environment.

According to another specific embodiment of the present invention, the accommodating cavity 210 is further provided with a nucleic acid removing part 220 , and the nucleic acid removing part 220 can be opened to allow the destruction liquid to flow into the accommodating cavity 210 . Use nucleic acid destruction reagents to remove residual nucleic acids in the detection cartridge, further avoiding possible contamination in subsequent processes.

According to another specific embodiment of the present invention, the housing 200 is provided with a accommodating cavity 210, and the accommodating cavity 210 is provided with a nucleic acid removal part 220, a detection cardboard 240 and a tube breaking part 250 in order from bottom to top, and the nucleic acid removal part The upper surface of 220 is provided with an opening, and the opening is sealed by a liquid seal 230, the liquid seal 230 can be opened, and the nucleic acid removal part 220 can store nucleic acid destruction reagents, such as sodium hypochlorite solution or commercial DNA detergents.

The upper surface of the housing 200 is provided with an intubation port 260 and a detection result observation area 270. The intubation port 260 includes a cylindrical first channel, the first channel includes an insertion end and a non-insertion end, and the insertion end is configured for gene amplification. The extension tube 100 is inserted, and the shape of the insertion end matches the gene amplification tube 100 to seal the accommodating cavity 210. The insertion end of the first channel is provided with an annular first groove 261. When the gene amplification tube 100 is inserted into the first channel In the channel, the first convex portion 111 and the first groove 261 cooperate with each other to limit the axial movement of the gene amplification tube 100 relative to the first channel.

As shown in FIG. 7 and FIG. 8 , the broken tube portion 250 includes a cylindrical positioning portion 251 and a piercing portion 252 located on the central axis of the positioning portion 251. The broken tube portion 250 is further provided with a fluid channel 253. The non-insertion end is provided with a third limiting portion 262 , and the positioning portion 251 is provided with a fourth limiting portion 2511 . The central axis is the rotation axis, the inner surface of the positioning portion 251 is provided with an inner thread (not shown in the figure), and the outer surface of the gene amplification tube 100 is provided with an outer thread matched with the inner thread.

When the gene amplification tube 100 is inserted into the first channel and the gene amplification tube 100 is rotated, as shown in FIG. 3 , since the first convex portion 111 fits with the annular first groove 261 , the gene amplification tube 100 can only rotated without being able to be inserted inwardly relative to the first channel. And as the gene amplification tube 100 rotates, since the outer thread on the side wall 110 of the gene amplification tube 100 is matched with the inner thread on the positioning part 251 , the positioning part 251 will drive the piercing part 252 to the gene amplification tube 100 . Move to the position shown in FIG. 4 (in FIG. 4 , the liquid seal 230 has been opened, but the liquid seal 230 cannot be opened before the detection is completed, only the broken tube 250 and the gene amplification tube 100 are shown here in FIG. 4 . relative positional relationship), the gene amplification tube 100 is punctured, and the nucleic acid solution flows into the accommodating cavity 210 from the fluid channel 253 . The detection card 240 in the accommodating cavity 210 can detect the sample, and the detection personnel can observe the detection result through the detection result observation area 270 .

With the above technical solution, during the detection process, the accommodating cavity 210 is always kept sealed to prevent the amplification product from leaking to the outside. Furthermore, after recording the detection results, as shown in FIG. 4 , the liquid seal 230 can be opened, and the detection card 240 can react with the nucleic acid destroying reagent to completely remove the nucleic acid remaining in the detection cartridge. In this way, even in the subsequent process, the gene amplification tube 100 falls off accidentally, or the detection cartridge is damaged, resulting in internal exposure, without causing any pollution.

In addition, in the prior art in which the gene amplification tube 100 is inserted downward while the tube breaking portion 250 remains stationary, since the gas in the casing 200 is compressed, amplification products may be amplified from the gene. There is leakage in the gap where the tube 100 and the casing 200 are connected, causing contamination. However, through the closed detection cartridge with a new structure provided by the present invention, the device allows the gene amplification tube 100 to remain stationary in the axial direction, but to puncture the gene amplification tube 100 by rotating the tube breaking part 250 to move upward. , During the puncturing process, the air pressure in the casing 200 remains unchanged, and the aerosol of the amplification product will not leak to the outside, which can better avoid contamination. In addition, the gene amplification tube 100 and the housing 200 are connected by threads, the connection structure is more stable, and it is not easy to fall off, which further avoids the exposure of the amplification product and the possibility of contamination. And finally, the nucleic acid destruction reagent is used to remove the residual nucleic acid in the detection cartridge, so as to further avoid possible contamination in the subsequent process.

According to another specific embodiment of the present invention, the third limiting portion 262 is a second groove extending in the axial direction, and the fourth limiting portion 2511 is a second convex portion. The second convex portion may be a convex point or a convex strip extending in the axial direction; and the length of the second groove should allow the breaking tube portion 250 to move upward to the position where the gene amplification tube 100 is pierced. Before the gene amplification tube 100 is inserted into the positioning portion 251 and starts to rotate, the second convex portion is located at the lowermost end of the second groove. Move upward along the second groove until the broken tube part 250 pierces the bottom tube wall 120 of the gene amplification tube 100 .

Further, from a more reliable point of view, a plurality of second protrusions and a second groove extending along the axial direction can be provided. Preferably, a plurality of second protrusions and a matching groove along the axis The second extending grooves are uniformly distributed along the circumference of the first channel.

It is easily conceivable that the third limiting portion 262 can also be set as a second convex portion, and the fourth limiting portion 2511 can be set as a second groove extending in the axial direction.

According to another specific embodiment of the present invention, as shown in FIG. 3 to FIG. 6 , the casing 200 is provided with a through hole, and the liquid seal 230 is penetrated on the casing 200 through the through hole. The operating part and the sealing part located in the housing 200, the sealing part seals the upper surface of the nucleic acid removal part 220, and the liquid sealing member 230 has a first position and a second position, when the detection cartridge is not in use, the liquid sealing member 230 is located in Fig. In the first position shown in 3, through the operation part, the liquid seal 230 can be moved to the second position shown in FIG. 4, and the nucleic acid removal part 220 can be opened. During the whole process, the liquid seal 230 is always sealed with the housing 200. , the accommodating cavity 210 is always in a sealed state, and the amplification product will not leak.

According to another specific embodiment of the present invention, the lower end of the positioning portion 251 is provided with a third convex portion 2512, and the liquid sealing member 230 is provided with a corresponding third groove. Before the broken pipe portion 250 moves upward, the third convex portion 2512 is disposed in the third groove. Due to the cooperation between the third convex portion 2512 and the third groove, the liquid seal 230 is always in the first position and cannot be moved to the second position. Insert the positioning portion 251 into the gene amplification tube 100 and rotate and lift the tube breaking portion 250 Afterwards, the third protruding portion 2512 also moves upwards and disengages from the third groove. At this time, the testing personnel can move the liquid sealing member 230 from the first position to the second position through the operation part, open the nucleic acid removal part 220 , and the detection cardboard 240 can be in contact with the destruction liquid in the nucleic acid removal part 220 .

According to another specific embodiment of the present invention, an elastic depression structure 280 is disposed above the detection cardboard 240 . When the liquid seal 230 is opened, the elastic depression structure 280 presses at least part of the detection cardboard 240 into the nucleic acid removal part 220 . The elastic pressing structure 280 is not particularly limited, and may be a spring or an elastic sheet.

Further, the specific structure for realizing the sealing of the accommodating cavity 210 can refer to any existing method in the prior art, which is not repeated in the present invention. After the gene amplification tube 100 is inserted into the intubation port 260 , the surfaces of the two are abutted to achieve the sealing of the accommodating cavity 210 . According to another specific embodiment of the present invention, in order to more effectively prevent the expansion product from leaking into the air, a sealing ring 140 made of elastomer may also be provided at the insertion end of the first channel, or the side of the gene amplification tube 100 may be provided A sealing ring 140 made of elastomer is provided outside the pipe wall 110 .

According to another specific embodiment of the present invention, the detection result observation area 270 is made of transparent material.

Although the present invention has been illustrated and described by referring to some preferred embodiments of the present invention, those of ordinary skill in the art should understand that the above content is a further detailed description of the present invention in conjunction with specific embodiments, and it cannot be assumed that Embodiments of the present invention are limited only by these descriptions. Those skilled in the art may make various changes in form and details, including making several simple deductions or substitutions, without departing from the spirit and scope of the present invention.

Claims (7)

1. The utility model provides a gene amplification tube, its characterized in that, includes body and lid, open at the body top has mouth of pipe and bottom seal, the lid is sealed the top mouth of pipe of body, the body includes side pipe wall and bottom tube wall, the side pipe wall includes cylindrical side pipe wall and is located the toper side pipe wall of cylindrical side pipe wall below, be equipped with on the bottom tube wall to the sunken depressed area in the body inside, the thickness of the bottom pipe wall in the depressed area is less than the thickness of side pipe wall.

2. The gene amplification tube of claim 1, wherein the tapered side wall has a thickness less than that of the cylindrical side wall, and the bottom wall in the recessed region has a thickness less than that of the tapered side wall.

3. The gene amplification tube of claim 1, wherein the tapered side wall has a thickness of less than 0.3 mm.

4. The gene amplification tube of claim 1, wherein the thickness of the wall of the bottom tube in the recessed region is less than 0.3 mm.

5. The gene amplification tube of claim 1, wherein the thickness of the bottom wall in the recessed region is 0.1 to 0.2 mm.

6. A detection card box, characterized by, including shell and the gene amplification tube of any one of claims 1-5, be equipped with the holding chamber in the shell, the shell upper surface has seted up the intubate mouth, holding intracavity below the intubate mouth from the bottom up is equipped with detection card paper and broken pipe portion in proper order, after the gene amplification tube inserted the intubate mouth, the holding chamber is sealed, broken pipe portion punctures the gene amplification tube, nucleic acid solution flows into the holding chamber.

7. The detection cartridge of claim 6, wherein the receiving chamber further comprises a nucleic acid removing portion, the nucleic acid removing portion being openable to allow the destructive liquid to flow into the receiving chamber.

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