CN111840123A - Application of glycyrrhetinic acid in hair blackening - Google Patents
Application of glycyrrhetinic acid in hair blackening Download PDFInfo
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- CN111840123A CN111840123A CN202010896729.7A CN202010896729A CN111840123A CN 111840123 A CN111840123 A CN 111840123A CN 202010896729 A CN202010896729 A CN 202010896729A CN 111840123 A CN111840123 A CN 111840123A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/63—Steroids; Derivatives thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/56—Compounds containing cyclopenta[a]hydrophenanthrene ring systems; Derivatives thereof, e.g. steroids
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P17/00—Drugs for dermatological disorders
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q5/00—Preparations for care of the hair
- A61Q5/02—Preparations for cleaning the hair
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q5/00—Preparations for care of the hair
- A61Q5/10—Preparations for permanently dyeing the hair
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q5/00—Preparations for care of the hair
- A61Q5/12—Preparations containing hair conditioners
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/74—Biological properties of particular ingredients
- A61K2800/78—Enzyme modulators, e.g. Enzyme agonists
Abstract
The invention relates to the technical field of cosmetics and discloses application of glycyrrhetinic acid in hair blacking or prevention and/or treatment of premature graying of hair. And in particular discloses a hair-blacking method, a composition with a hair-blacking function and a hair care product with a hair-blacking function. The method provided by the invention can effectively improve the early-whitening symptom of hair, and has the advantages of quick response, good effect and no obvious stimulation or toxic and side effect on scalp and hair follicle.
Description
Technical Field
The invention relates to the technical field of cosmetics, in particular to application of glycyrrhetinic acid in hair blacking or prevention and/or treatment of premature graying of hair and preparation of a hair care product with a hair blacking function, a hair blacking method, a composition with a hair blacking function and a hair washing and caring product with a hair blacking function.
Background
With the continuous improvement of the living standard of the modern society, the life rhythm of people is gradually accelerated, and the white hair phenomenon is gradually younger caused by the problems of increased living pressure, irregular diet, environmental pollution and the like. The early graying of hair not only brings serious influence to the appearance of people, but also causes great stress to the spirit and the mind of people. The white hair is caused by the reduction or air-filling of melanin granules in the hair's medulla and cortex. Under normal conditions, the inside of the hair papilla is rich in blood vessels, so that sufficient nutrition is provided for the hair papilla and hair bulb, and melanin granules are smoothly synthesized. When melanin granules are formed in the papilla or the bulbar portion of the hair, and are not transported to the hair due to some factors, thereby reducing or eliminating melanin granules in the medullary or cortex portion of the hair, there occurs a problem that the hair is early white, for example, gray, grayy, white, etc. At present, the treatment means for early graying of hair is limited, and the problems of high cost, slow effect, poor effect, easy repetition and the like exist. In addition, most people suffering from the problem of early graying of hair choose to dye hair in order to meet the requirement of beauty treatment, but frequent hair dyeing is expensive, and most of hair dyes sold in the market at present contain aniline substances, so that allergy is easily caused, the hair dyes can further damage the scalp and hair follicles, the disease condition is aggravated, and other hair problems such as alopecia are caused. Therefore, a new hair-blackening product is needed to solve the problem of early-whitening of hair economically, quickly and effectively.
Glycyrrhetinic acid (also called Glycyrrhetinic acid) is a triterpenoid saponin compound extracted from root and rhizome of Glycyrrhiza uralensis Fisch (Glycyrrhexis glabra L.) of Leguminosae, and has the following structural formula:
glycyrrhetinic acid has antibacterial and antiinflammatory effects, can inhibit capillary permeability, has certain antiallergic effect, and has the advantages of high sebum removing efficiency, no irritation to skin, and wide application range. Therefore, glycyrrhetinic acid is commonly used for preparing anti-inflammatory, antibacterial, and antiallergic drugs, or added to skin care products, oral hygiene products, lipsticks, and facial cleansing products. At present, no record exists that glycyrrhetinic acid can be used in hair blackening products.
Disclosure of Invention
The invention aims to overcome the problems of slow effect, poor effect and the like of hair blackening products in the prior art, provides the application of glycyrrhetinic acid in hair blackening or prevention and/or treatment of premature graying of hair and preparation of hair washing and/or caring products with the hair blackening function, and provides a hair blackening method, a composition with the hair blackening function and a hair washing and caring product with the hair blackening function. By adopting the technical scheme provided by the invention, the conditions of hair follicles and hair roots can be obviously improved, and a good basic environment is provided for synthesis and transportation of melanin particles, so that the early-whitening symptom of hair is improved, and the hair blackening agent has the advantages of good hair blackening effect, no obvious irritation to scalp and the like.
In order to achieve the above objects, the present invention provides, in one aspect, use of glycyrrhetinic acid for blackening hair or preventing and/or treating premature graying of hair.
In a second aspect, the present invention provides the use of glycyrrhetinic acid in the preparation of a hair washing and/or caring product having a hair-blacking function.
In a third aspect of the invention there is provided a method of darkening hair, the method comprising contacting glycyrrhetinic acid, or a hair washing and/or care product containing glycyrrhetinic acid, with the skin of the hair in which the hair is located.
The invention provides a composition with a hair blacking function, which comprises glycyrrhetinic acid, auxiliary materials and a solvent.
The fifth aspect of the invention provides a washing and caring product with a hair blackening function, which comprises an active ingredient, an auxiliary material and a solvent, wherein the active ingredient is glycyrrhetinic acid.
According to a sixth aspect of the present invention, there is provided the use of glycyrrhetinic acid, a composition as described above and a lotion product as described above for activating tyrosinase or promoting melanin production or for preventing and/or treating premature graying of hair, in particular for activating tyrosinase or promoting melanin production in vitro.
Through the technical scheme, the invention can obtain the following beneficial technical effects:
(1) the washing and protecting product with the hair blackening function provided by the invention adopts the glycyrrhetinic acid as a main functional component, is mild, does not stimulate and damage scalp and hair follicles, can effectively avoid other secondary hair problems such as hair loss, hair follicle damage and the like compared with hair dyeing, and does not generate toxic or side effect on human bodies;
(2) the washing and caring product with the hair blackening function provided by the invention directly acts on the scalp, activates tyrosinase, induces the synthesis of melanin in scalp cells and hair follicles, and improves the melanin transportation condition. Compared with the traditional Chinese medicine or health food, the product has quick response (the hair of a guinea pig test area can be observed to turn black after the guinea pig is treated by glycyrrhetinic acid for 8 days in a guinea pig experiment), and the hair blackening effect is good;
(3) the glycyrrhetinic acid directly contacts with the scalp by the method and/or the washing and caring product provided by the invention, the tyrosinase in hair follicles is activated, the synthesis of melanin is induced, the melanin transportation condition is improved, and the early-white problems of hair (such as grey hair, full white hair and the like) of various degrees can be effectively relieved and improved.
Detailed Description
While specific embodiments of the present invention will be described and illustrated below, it should be understood that the following specific embodiments are only illustrative and explanatory of the invention, and are not restrictive thereof.
The endpoints of the ranges and any values disclosed herein are not limited to the precise range or value, and such ranges or values should be understood to encompass values close to those ranges or values. For ranges of values, between the endpoints of each of the ranges and the individual points, and between the individual points may be combined with each other to give one or more new ranges of values, and these ranges of values should be considered as specifically disclosed herein.
Interpretation of terms:
in the present invention, unless otherwise specified, the term "blacking hair" means: so as to relieve and improve the problems of gray hair, gray white hair, white hair and the like caused by the deficiency of melanin in the hair and restore the natural blackness of the hair.
In the present invention, unless otherwise specified, the term "premature graying of hair" means: the problems of grey, grey and white hair and the like caused by insufficient synthesis of melanin in hair follicles and/or unsmooth melanin transportation paths.
The inventor of the invention skillfully discovers in the research process that glycyrrhetinic acid can activate tyrosinase, is beneficial to melanin synthesis, can induce hair follicles to enter the hair growth period, has no irritation and sensitization to skin and hair follicles, and is very suitable for developing novel hair-blacking products.
The invention provides, in a first aspect, the use of glycyrrhetinic acid for blackening hair or for preventing and/or treating premature graying of hair, in particular for blackening hair for non-therapeutic purposes.
According to a preferred embodiment of the present invention, wherein the application may include development of hair-blacking products such as hair-care products, external medicines, and the like. Also included is the study of a method of treating premature graying of hair using glycyrrhetinic acid.
According to a preferred embodiment of the present invention, wherein the blackening or preventing and/or treating of hair premature graying comprises: improve the problems of gray hair, gray white hair, white hair and the like caused by the reduction of melanin in the hair, and restore the natural black hair.
In a second aspect, the present invention provides the use of glycyrrhetinic acid in the preparation of a hair care product having a hair-blacking function.
According to a preferred embodiment of the present invention, the hair care product may include at least one of a shampoo product, a hair care essence, and a hair tonic.
According to a preferred embodiment of the present invention, the hair blackening function comprises improving the early-white problem of hair (such as grey, white, etc.) and restoring natural hair blackening.
In a third aspect the present invention provides a method of blacking hair (particularly a non-therapeutic hair blacking method) comprising contacting glycyrrhetinic acid or a hair care product containing glycyrrhetinic acid with the skin on which the hair is located.
According to a preferred embodiment of the present invention, wherein the composition and/or hair care product is used in an amount of 0.05-10mg/cm, calculated as glycyrrhetinic acid2. Preferably 0.05-6.5mg/cm2。
According to a preferred embodiment of the present invention, wherein the composition and/or hair care product is applied at a frequency of 16 to 24 h/time.
The invention provides a composition with hair blacking function, which contains glycyrrhetinic acid, auxiliary materials and solvents.
According to a preferred embodiment of the present invention, the composition can be used for preparing a leave-on hair care product having a hair blackening function, such as hair essence, hair conditioner, hair mask, etc.
According to a preferred embodiment of the present invention, wherein the glycyrrhetinic acid is contained in an amount of 0.01 to 1% by weight, based on the total weight of the composition.
According to a preferred embodiment of the present invention, wherein the content of the adjuvant (solubilizer) is 0.05 to 0.5% by weight, based on the total weight of the composition.
According to a preferred embodiment of the present invention, wherein the solvent is contained in an amount of 98.5 to 99.94% by weight, based on the total weight of the composition.
According to a preferred embodiment of the present invention, wherein the composition contains glycyrrhetinic acid only as an active ingredient.
According to a preferred embodiment of the present invention, wherein the auxiliary material may be a solubilizer.
Preferably, the solubilizer is selected from at least one of hydrogenated castor oil, polysorbate, and butanol polyether.
More preferably, the solubilizer is a mixture of hydrogenated castor oil and polysorbate, wherein the weight ratio of hydrogenated castor oil to polysorbate is 1-2: 1.
According to a preferred embodiment of the present invention, wherein the hydrogenated castor oil is at least one selected from the group consisting of hydrogenated castor oil PEG-40 and hydrogenated castor oil PEG-60.
According to a preferred embodiment of the present invention, wherein the polysorbate is at least one selected from polysorbate-20 and polysorbate-80.
According to a preferred embodiment of the present invention, wherein the solvent is at least one selected from the group consisting of ethanol, water and propylene glycol.
Preferably, the solvent is a mixture of ethanol and water, wherein the weight ratio of ethanol to water is 1: 2-5.
The fifth aspect of the invention provides a hair washing and caring product with a hair blackening function, which comprises an active ingredient, an auxiliary material and a solvent, wherein the active ingredient is glycyrrhetinic acid.
The hair washing and caring product can be any form of hair washing and caring product existing in the field, for example, shampoo, hair lotion, hair conditioner, hair care essence, etc.
According to a preferred embodiment of the present invention, wherein the auxiliary material is selected from at least one of a surfactant, a viscosity modifier, a humectant, a stabilizer, a preservative, a conditioning agent, and an acid-base modifier.
According to a preferred embodiment of the present invention, wherein the solvent is at least one selected from the group consisting of water, ethanol and a polyhydric alcohol having 3 to 6 carbon atoms.
Preferably, the solvent is selected from at least one of water, ethanol and propylene glycol.
According to a preferred embodiment of the present invention, wherein the glycyrrhetinic acid is contained in an amount of 0.01 to 3 wt% based on the total weight of the hair wash and care product. Preferably 0.01 to 1% by weight.
According to a preferred embodiment of the present invention, the content of the auxiliary materials is 10 to 30 wt%, preferably 10 to 20 wt%, based on the total weight of the hair washing and caring product.
Preferably, the surfactant is present in an amount of 5 to 25 wt.%, based on the total weight of the hair wash and care product.
Preferably, the viscosity modifier is present in an amount of 0.1 to 1 wt%, based on the total weight of the hair wash and care product.
Preferably, the humectant is present in an amount of 1 to 5 wt%, based on the total weight of the hair wash and care product.
Preferably, the stabilizer is contained in an amount of 0.01 to 0.8 wt% based on the total weight of the hair wash and care product.
Preferably, the preservative is present in an amount of 0.1 to 1% by weight, based on the total weight of the hair care product.
Preferably, the conditioning agent is present in an amount of 0.1 to 5 wt%, based on the total weight of the hair wash and care product.
Preferably, the content of the acid-base regulator is 0.02-3 wt% based on the total weight of the hair washing and caring product.
More preferably, the surfactant is present in an amount of 10 to 15 wt.%, based on the total weight of the hair wash and care product.
More preferably, the viscosity modifier is present in an amount of 0.2 to 0.5 wt.%, based on the total weight of the hair wash and care product.
More preferably, the humectant is present in an amount of 1.8 to 2.5 wt%, based on the total weight of the hair wash and care product.
More preferably, the stabilizer is present in an amount of 0.05 to 0.2 wt%, based on the total weight of the hair wash and care product.
More preferably, the preservative is present in an amount of 0.5 to 1.5% by weight, based on the total weight of the hair wash product.
More preferably, the conditioning agent is present in an amount of from 0.5 to 3% by weight, based on the total weight of the hair wash and care product.
More preferably, the content of the acid-base regulator is 0.1-1 wt% based on the total weight of the hair washing and caring product.
Any surfactant known in the art may be suitable for use in the rinse and hair care products provided herein. According to a preferred embodiment of the present invention, wherein, in the hair care product, the surfactant is selected from at least one of sodium laureth sulfate, lauramidopropyl hydroxysultaine and cocamidomethylethanolamine.
According to a preferred embodiment of the present invention, wherein, in the hair care product, the surfactant is a mixture of sodium laureth sulfate, lauramidopropyl hydroxysultaine and cocamidomethylethanolamine.
Preferably, the content of the sodium laureth sulfate is 5-10 wt%, the content of the sodium laureth sulfate is 1-3 wt%, the content of the lauramidopropyl hydroxysultaine is 1-5 wt%, and the content of the cocamidomethylethanolamine is 0.1-1.5 wt%, based on the total weight of the hair care product.
More preferably, the sodium laureth sulfate is present in an amount of from 8 to 10 wt%, the sodium laureth sulfate is present in an amount of from 1 to 2 wt%, the lauramidopropyl hydroxysultaine is present in an amount of from 2 to 3 wt%, and the cocamidomethylethanolamine is present in an amount of from 0.5 to 1 wt%, based on the total weight of the hair wash and care product.
Any viscosity modifier known in the art may be suitable for use in the rinse and hair care products provided herein. According to a preferred embodiment of the present invention, wherein, in the hair care product, the viscosity modifier is selected from at least one of sodium chloride, ammonium chloride, hydroxypropyl methylcellulose and carbomer.
According to a preferred embodiment of the present invention, wherein, in the hair care and wash product, the viscosity modifier is sodium chloride; preferably, the sodium chloride is present in an amount of 0.3 to 0.5 wt.%.
Any moisturizer known in the art may be suitable for use in the hair care products provided herein. According to a preferred embodiment of the present invention, wherein, in the hair care product, the moisturizer is at least one selected from diglycerin, glycerin and squalane.
According to a preferred embodiment of the present invention, in the hair care product, the humectant is a mixture of diglycerin and glycerin.
Preferably, the diglycerin is contained in an amount of 1-5 wt% based on the total weight of the hair care product. The content of the glycerol is 0.01-0.5 wt%.
More preferably, the diglycerin is present in an amount of 1.5 to 2.5 wt%, based on the total weight of the hair care and wash product. The content of the glycerol is 0.05-0.15 wt%.
Any stabilizer known in the art may be suitable for use in the rinse and hair care products provided herein. According to a preferred embodiment of the present invention, wherein, in the hair care product, the stabilizer is at least one selected from disodium ethylenediaminetetraacetate (EDTA-2Na), tetrasodium ethylenediaminetetraacetate (EDTA-4 Na).
According to a preferred embodiment of the present invention, wherein, in the hair care and washing product, the stabilizer is EDTA-2 Na; preferably, the EDTA-2Na is contained in an amount of 0.05 to 0.15 wt%.
Any preservative known in the art may be suitable for use in the rinse and hair care products provided herein. According to a preferred embodiment of the present invention, wherein, in the hair care product, the preservative is at least one selected from phenoxyethanol, sodium benzoate and paraben.
According to a preferred embodiment of the present invention, wherein, in the hair care and washing product, the preservative is phenoxyethanol. Preferably, the content of the phenoxyethanol is 0.4 to 0.6 percent by weight.
Any conditioning agent known in the art may be suitable for use in the rinse and hair care products provided herein. According to a preferred embodiment of the present invention, wherein, in the hair care product, the conditioning agent is at least one selected from the group consisting of polyquaternium-10 and polyquaternium-7.
According to a preferred embodiment of the present invention, wherein, in the hair care and washing product, the conditioning agent is polyquaternium-10. Preferably, the polyquaternium-10 is present in an amount of 0.3 to 0.5 wt%.
Any preservative known in the art may be suitable for use in the rinse and hair care products provided herein. According to a preferred embodiment of the present invention, wherein, in the hair care and washing product, the pH regulator is at least one selected from citric acid, lactic acid and arginine.
According to a preferred embodiment of the present invention, in the hair washing and caring product, the pH regulator is citric acid. Preferably, the citric acid is contained in an amount of 0.01 to 0.1 wt%.
According to a sixth aspect of the present invention, there is provided the use of glycyrrhetinic acid, a composition as described above and a hair wash and care product as described above for activating tyrosinase or promoting melanin production or for preventing and/or treating premature graying of hair, in particular for activating tyrosinase or promoting melanin production in vitro.
The present invention will be described in detail below by way of examples. In the following examples, glycyrrhetinic acid was purchased from Beijing Chenfeng corporation and had a purity of 98% (by titration). PBS was purchased from TaKaRa. Tyrosinase and levodopa were purchased from SIGMA. The absorbance was measured by a microplate reader model Synergy LX from BioTek.
Example 1
This example illustrates the activation of tyrosinase by glycyrrhetinic acid.
Preparing 200U/mL tyrosinase solution and 10mM levodopa solution by using a phosphate buffer solution with the pH value of 6.8, and placing the prepared tyrosinase solution on ice for later use.
Glycyrrhetinic acid solutions of 0.25mg/mL, 0.5mg/mL and 1mg/mL were prepared using a phosphate buffer solution having a pH of 6.8, respectively.
To each well of the 96-well plate, 100. mu.L of a 10mM levodopa solution and 100. mu.L of a glycyrrhetinic acid solution were added, and the mixture was allowed to stand at room temperature for 5 min. Adding 100 mu L of tyrosinase solution of 200U/mL, immediately mixing, placing in a microorganism incubator at 37 ℃ for 25min, and measuring the absorbance OD value of each hole at 475nm of a microplate reader. Each set of samples was repeated 3 times. The tyrosinase activation rate of the sample was calculated as follows:
tyrosinase activation rate (%) - (OD)3－OD4)－(OD1－OD2)]/(OD1－OD2)×100％
OD1The absorbance measured by the mixed solution with enzyme but without the sample to be measured;
OD2absorbance measured by a mixed solution without adding a sample to be measured or an enzyme;
OD3adding the absorbance measured by the mixed solution of the sample to be measured and the enzyme;
OD4the absorbance measured by the mixed solution added with the sample to be measured and without the enzyme.
See table 1 for specific experimental results.
Example 2
This example illustrates the effect of glycyrrhetinic acid in promoting melanin production.
The density of the B16 melanoma cell suspension was adjusted to 1X 1051 mL/mL of the culture medium was inoculated into a 12-well plate, and the cell density was adjusted to 5X 104Each well was cultured in an incubator (37 ℃ C., 5% carbon dioxide) for 24 hours. And taking out the 12-hole plate, removing the culture medium, adding 1mL of culture medium containing the sample to be detected, adding 1mL of culture medium containing no sample to be detected into the blank control group and the zero adjustment group, continuously placing the blank control group and the zero adjustment group in an incubator for culturing for 12 hours, and removing the culture medium. The experimental group and the zero-setting group were added with 1mL of 0.25mg/mL, 0.5mg/mL, and 1mg/mL glycyrrhetinic acid solution prepared with RPMI1640 medium, respectively, and the blank group was added with 1mL of RPMI1640 medium (containing no glycyrrhetinic acid). After culturing for 36 hours in an incubator, the medium was aspirated, washed twice with 1mL of PBS, 100. mu.L of a 1M NaOH solution containing 10% DMSO (prepared with PBS) was added to each well, and after water bath for 1 hour at 80 ℃, the cell lysates of the respective groups in the 12-well plate were transferred to a 96-well plate, and the absorbance OD of each well was measured at 475nm using a microplate reader. The formula for calculating the intracellular melanin content is as follows:
intracellular melanin content ═ OD5-OD7)/(OD6-OD7)×100％
OD5: absorbance values of the test groups
OD6: absorbance values of blank control
OD7: absorbance values of zeroing groups
See table 1 for specific experimental results.
TABLE 1 Glycyrrhetinic acid in vitro test results
| Glycyrrhetinic acid dosage (mg/ml) | Tyrosinase activation rate (%) | Melanin content (%) |
| 1 | 388.45±16.24 | 2.12±0.34 |
| 0.5 | 245.12±8.46 | 1.22±0.26 |
| 0.25 | 126.21±6.21 | 0.61±0.03 |
The color of hair depends mainly on the content of melanocytes and melanin in the hair bulb and papilla. Melanocytes, which are mainly present in the basal layer of the epidermis and hair follicles, among which tyrosinase is the most important factor determining melanin synthesis. The early formation of hair graying is mostly caused by the decrease of melanocytes and melanosomes or the decrease of tyrosinase activity causing melanin synthesis disorders.
As can be seen from the results in table 1, glycyrrhetinic acid has a good activating effect on tyrosinase, promoting melanin synthesis; and the effect is more obvious along with the increase of the concentration in a certain concentration.
Example 3
This example uses animal experiments to demonstrate the hair-blacking effect of glycyrrhetinic acid.
Selecting 45 healthy black and colored guinea pigs with the weight of 300-: normal control group (no treatment); group B: model control group (no drug after molding); group C: glycyrrhetinic acid experimental group (drug application after molding). Groups B-C the guinea pig test area was overcoated with 5% hydrogen peroxide for 40 days 2 times a day with 2ml each time, and the model was made (the guinea pig hair was made white). After the molding is successful, the group C is coated with 0.1 wt% glycyrrhetinic acid 1 time per day, 2ml each time, and continuously coated for 30 days.
After the modeling experiment is finished, one guinea pig in each group is randomly cut into skin tissues in an experimental area with a proper size to be pathological sections, and the melanin changes of the skin tissues and hair follicles are observed. The pathological section observation skin tissue and hair follicle melanin change method: cutting skin tissue of an experimental area with proper size, fixing with 10% formaldehyde, dehydrating the conventional tissue, embedding paraffin, HE staining, performing light microscopic examination, and observing indexes related to hair follicle melanin growth of the skin tissue of the guinea pig. ② the dermis thickness measurement, the skin is thickened in the hair growth period, so the measurement of the dermis thickness (the distance between a basement membrane and a muscle membrane) can indirectly reflect the hair growth condition. And counting hair follicles, namely counting the number of the hair follicles in a high-power visual field (200 times). The number of capillaries in the dermis layer is 200 times that in the high visual field. Counting the number of cells containing melanin granules per 100 basal cells under a high power lens (400 times). Sixthly, observing 50 hair follicles at random under a high power microscope (400 times), and counting the number of melanin hair follicles.
The center of the selected application part of other guinea pigs is 4cm2The effect is judged in the observation area. The criteria for determination are as follows: "you" is the pigment recovery area in the observation area and the black hair in the new hair>75 percent; "good" refers to the area of pigment appearing in the observation area and new black hair>50 percent; the middle part is the area where pigment appears in the observation area and new black hair appears>25 percent; "poor" is a pale or leukoplakia, hairless growth or new white vellus hair in the observation area. The total effective rate is calculated by the proportion of 'excellent' and 'good'.
The results of this example are as follows:
the results of the general histological examination show that the test area is completely covered by squamous epithelium, the keratinized layer is not thickened, the dermis is not provided with vasodilatation congestion, inflammatory cell infiltration and the like, which indicates that the guinea pig skin has complete structure, and the glycyrrhetinic acid has no stimulation to the skin and is safe to use.
The results of observation of the effect of glycyrrhetinic acid on improving early graying of hair are shown in table 2, and the results of the effect on the skin and hair follicle melanin of guinea pigs are shown in table 3.
TABLE 2 Observation of Effect of Glycyrrhetinic acid on improving premature graying of Hair
TABLE 3 Effect of Glycyrrhetinic acid on Guinea pig skin and Hair follicle Melanin
| Group of | n | Number of basal cells containing melanin granules | Hair follicle number with melanin |
| Group A | 15 | 78±2.12 | 45.93±2.63 |
| Group B | 15 | 31.1±2.6 | 22.1±3.68 |
| Group C | 15 | 76.1±7.52 | 43.1±2.69 |
As can be seen from the results of tables 2 and 3, the total effective rate of improving hair whitening in the guinea pigs in the glycyrrhetinic acid-applied experimental group was significantly higher than that in the model control group, and the number of basal cells containing melanin granules and the number of hair follicles containing melanin in the skin sections were also higher than those in the model control group. Therefore, the glycyrrhetinic acid has a good effect of improving the early-graying condition of hair.
Example 4
This example illustrates the hair-blackening effect of the wash care product provided by the present invention.
Guinea pigs were molded according to the method of example 3, and hair-blackening effect experiments were performed on the successfully molded guinea pigs using the hair-blackening shampoos in table 4. The specific method comprises the following steps: guinea pigs were divided into three groups, group a being a normal control group (without any treatment); group B was a model control group (no drug was used after molding); group C: glycyrrhetinic acid experimental group (drug application after molding). After the group B-C modeling is successful, the group B washes the hair of the tested area once by warm water every 24 hours for 90 days continuously; in group C, the hair of the test area was washed once every 24 hours with 2mL of shampoo of the formula in Table 4 for 90 consecutive days.
The above experiment achieved similar effects as in example 3. The percentage of black hair in the new hair was significantly increased in the guinea pigs of group C compared to the guinea pigs of group B. Therefore, the hair blackening shampoo provided by the invention has a good effect of improving the early graying of hair. In the experimental process, no obvious anaphylactic reaction occurs to the skin of the guinea pig, and the hair-blacking shampoo provided by the invention is proved to be mild and non-irritant to the skin.
TABLE 4 Glycyrrhetinic hair-blackening shampoo formula
The preferred embodiments of the present invention have been described above in detail, but the present invention is not limited thereto. Within the scope of the technical idea of the invention, many simple modifications can be made to the technical solution of the invention, including combinations of various technical features in any other suitable way, and these simple modifications and combinations should also be regarded as the disclosure of the invention, and all fall within the scope of the invention.
Claims (10)
1. Use of glycyrrhetinic acid for blackening hair or preventing and/or treating premature graying of hair.
2. Use of glycyrrhetinic acid in the preparation of a hair washing and/or caring product having hair blackening effect.
3. A method of darkening hair, the method comprising contacting glycyrrhetinic acid, or a hair washing and/or care product containing glycyrrhetinic acid, with the skin on which the hair is located.
4. The method according to claim 3, wherein said hair washing and/or hair care product is present in an amount of 0.05-10mg/cm, measured as glycyrrhetinic acid2；
And/or the frequency of use of the hair washing and/or hair care product is 16-24 h/time.
5. A composition with hair blackening function is characterized by comprising glycyrrhetinic acid, auxiliary materials and a solvent.
6. The composition of claim 5, wherein said glycyrrhetinic acid is present in an amount ranging from 0.01% to 1% by weight, based on the total weight of the composition;
and/or, the content of the auxiliary material is 0.05-0.5 wt% based on the total weight of the composition;
and/or, the solvent is present in an amount of 98.5 to 99.94% by weight, based on the total weight of the composition;
and/or, the composition contains glycyrrhetinic acid only as an active ingredient;
and/or the auxiliary material is a solubilizer;
and/or, the solvent is selected from at least one of ethanol, water and propylene glycol;
preferably, the solubilizer is selected from at least one of hydrogenated castor oil, polysorbate, and butanolpolyether;
more preferably, the solubilizer is a mixture of hydrogenated castor oil and polysorbate, wherein the weight ratio of hydrogenated castor oil to polysorbate is 1-2: 1;
more preferably, the solvent is a mixture of ethanol and water, wherein the weight ratio of ethanol to water is 1: 2-5;
further preferably, the hydrogenated castor oil is at least one selected from the group consisting of hydrogenated castor oil PEG-40 and hydrogenated castor oil PEG-60;
further preferably, the polysorbate is at least one selected from polysorbate-20 and polysorbate-80.
7. A hair washing and caring product with a hair blackening function is characterized by comprising an active ingredient, an auxiliary material and a solvent, wherein the active ingredient is glycyrrhetinic acid.
8. The hair wash and care product according to claim 7, wherein the auxiliary material is selected from at least one of a surfactant, a viscosity modifier, a humectant, a stabilizer, a preservative, a conditioning agent, and an acid-base modifier;
and/or the solvent is selected from at least one of water, ethanol and polyhydric alcohol with 3-6 carbon atoms;
preferably, the solvent is selected from at least one of water, ethanol and propylene glycol;
preferably, the surfactant is selected from at least one of sodium laureth sulfate, lauramidopropyl hydroxysultaine, and cocamidomethylethanolamine;
preferably, the viscosity modifier is selected from at least one of sodium chloride, ammonium chloride, hydroxypropyl methylcellulose, and carbomer;
preferably, the humectant is selected from at least one of diglycerin, glycerin, and squalane;
preferably, the stabilizer is at least one selected from disodium ethylene diamine tetraacetate and tetrasodium ethylene diamine tetraacetate;
preferably, the preservative is selected from at least one of phenoxyethanol, sodium benzoate and paraben;
preferably, the conditioning agent is selected from at least one of polyquaternium-10 and polyquaternium-7;
preferably, the pH adjusting agent is selected from at least one of citric acid, lactic acid and arginine.
9. The hair wash and care product according to claim 7 or 8, wherein the glycyrrhetinic acid is contained in an amount of 0.01 to 3 wt% based on the total weight of the hair wash and care product;
and/or, the content of the auxiliary material is 10-30 wt%;
preferably, the surfactant is contained in an amount of 5 to 25 wt% based on the total weight of the hair wash and care product;
preferably, the content of the viscosity regulator is 0.1-1 wt% based on the total weight of the hair washing and caring product;
preferably, the content of the humectant is 1-5 wt% based on the total weight of the hair washing and caring product;
preferably, the content of the stabilizer is 0.01 to 0.8 percent by weight based on the total weight of the hair washing and caring product;
preferably, the preservative is present in an amount of from 0.1 to 1% by weight, based on the total weight of the hair care product;
preferably, the content of the conditioning agent is 0.1-5 wt% based on the total weight of the hair washing and caring product;
preferably, the content of the acid-base regulator is 0.02-3 wt% based on the total weight of the hair washing and caring product.
10. Use of glycyrrhetinic acid, a composition according to claim 5 or 6 and a hair wash and care product according to any one of claims 7 to 9 for activating tyrosinase or promoting melanogenesis or for preventing and/or treating premature graying of hair, in particular for activating tyrosinase or promoting melanogenesis in vitro.
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