CN111837505B - Method for germinating cyperus esculentus seeds - Google Patents
Method for germinating cyperus esculentus seeds Download PDFInfo
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- CN111837505B CN111837505B CN202010732122.5A CN202010732122A CN111837505B CN 111837505 B CN111837505 B CN 111837505B CN 202010732122 A CN202010732122 A CN 202010732122A CN 111837505 B CN111837505 B CN 111837505B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
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Abstract
The invention discloses a method for germinating cyperus esculentus seeds. Belongs to the technical field of plant propagation. Comprises the following steps: pretreatment: cleaning cyperus esculentus seeds, and selecting full, non-pest, non-damaged and mature seeds for later use; nutrient solution soaking treatment: soaking the seeds in 6-BA solution; soaking the seeds in a 6-BA solution with the volume of 2-3 times of the seeds; soaking the seeds in purified water, adding EMS solution into the purified water to obtain a mixed solution, and mixing and soaking; culture in a culture medium: and (4) washing the seeds, and placing the seeds in a germination culture medium for germination. The method obviously improves the germination rate of the cyperus esculentus on the basis of keeping the quality of the cyperus esculentus, and has simple and easy-to-implement seed treatment method and good benefit.
Description
Technical Field
The invention relates to the technical field of plant propagation, in particular to a method for germinating cyperus esculentus seeds.
Background
Cyperus esculentus (Cyperus esculentus L.) is a plant of the genus Cyperus of the family Cyperaceae. The stem and leaf are clustered, the tillering force is strong, the stem is triangular, the stem is wrapped by the leaves, and the plant height can reach 100 cm. The single leaves are intergrown, the leaves are long and narrow, the average length is 65 cm, and the width is 0.5 cm. The stem and the fruit are oval, the length of the stem and the fruit is 1-2 cm, and the diameter of the stem and the fruit is 0.7-2 cm. Has knots and scales, and the scales at the bud end are fine and dense. And a small number of plants bloom, bracts grow in inflorescences, flowers grow at the top ends of main stems, the flowers are amphoteric, and each ear has 8-30 flowers.
Cyperus esculentus is originally produced in northern Africa and mediterranean coastal zones, and is widely cultivated in Spain, Italy, south Africa, south America and the like. The Beijing botanical garden of the institute of plant of Chinese academy of sciences in 1960 was successfully introduced by Bagalia and then successfully introduced in inner Mongolia, Liaoning, Guangdong, Guangxi, Fujian, Xinjiang, Gansu, etc. Belongs to positive plants, and is lucidient. Drought tolerance, waterlogging tolerance, barren tolerance and saline-alkali tolerance.
The cyperus esculentus is an economic crop integrating grain, oil, grazing and feeding, and has high quality, high yield and wide comprehensive utilization prospect. Is not only a good oil crop, but also a good feed for livestock, and can be used as a good pasture for development and planting. In addition, it can be used as lubricating oil and soap.
Before planting cyperus esculentus, seeds of the cyperus esculentus need to be treated, but after conventional treatment, the germination rate of the cyperus esculentus seeds is unstable, so that the yield of the cyperus esculentus is further influenced, the planting enthusiasm of part of growers is limited, and the economic benefit is reduced.
Therefore, how to provide a method for germinating cyperus esculentus seeds is a problem to be solved urgently by the technical personnel in the field.
Disclosure of Invention
In view of the above, the invention provides a method for germinating cyperus esculentus seeds, and by adopting the seed treatment method, the germination rate of the seeds can be improved, the acre yield of the cyperus esculentus can be improved, and the seedling death rate can be reduced.
In order to achieve the purpose, the invention adopts the following technical scheme:
a method for germinating cyperus esculentus seeds comprises the following steps:
(1) pretreatment: cleaning cyperus esculentus seeds, and selecting full, non-pest, non-damaged and mature seeds A for later use;
(2) nutrient solution soaking treatment:
21) soaking the seeds A in a 6-BA solution with the seed volume of 2-3 times to obtain seeds B;
22) soaking the seeds B in purified water C, adding EMS solution into the purified water C to obtain a mixed solution D, and mixing and soaking to obtain seeds E;
(3) culture in a culture medium: and (4) washing the seeds E, and placing the seeds E in a germination culture medium for germination.
Has the advantages that: firstly, cleaning and removing residual seeds, diseases or damaged seeds to create conditions for further nutrient solution soaking, and then carrying out continuous two-step nutrient soaking treatment to improve the germination rate of the cyperus esculentus seeds, earlier researches find that only 6-BA solution or only EMS solution is used for treating the seeds, which has various advantages and disadvantages, the improvement efficiency is not satisfactory only by using the 6-BA solution, but only EMS solution is used to inhibit the growth of the cyperus esculentus, and the plant height of the cyperus esculentus is greatly influenced at the later stage and is not beneficial to improving the yield, so that the continuous two-step nutrient soaking treatment is adopted; finally, the germination result is further stabilized through culture medium culture, and the occurrence of dead seedling is reduced.
Preferably: in the step 21), the concentration of the 6-BA solution is 60-80 mg/L, and the soaking time is 1-1.5 h.
Has the advantages that: the 6-BA solution is adopted to carry out primary treatment on the seeds, so that the germination efficiency is improved finally, the treatment time of the 6-BA solution is too long, the EMS solution treatment effect is reduced, and the pretreatment effect cannot be achieved due to too short treatment time.
Preferably: in the step 22), the pure water C is soaked for 15-18 h.
Has the advantages that: so that the seeds can absorb sufficient water, thereby facilitating the further EMS solution soaking treatment.
Preferably: in the step 22), the EMS solution concentration in the mixed solution D is 0.2%, and the mixing and soaking time is 4.5-5.5 h.
The method has the advantages that the EMS concentration is too high or too low, the soaking time is too long or too short, the germination of the cyperus esculentus seeds is not favorably improved, the EMS solution dosage and the concentration which are limited by the method are matched with the 6-BA solution soaking germination rate can reach more than 95%, and partial repeated experiments can reach 100% germination.
Preferably: in the step (3), the germination medium consists of the following components: 12-15 g/L sucrose, 100-150 mg/LGA3And 8-10 g/L agar.
Preferably: the culture conditions in the step (3) are as follows: at 25-30 ℃, the illumination intensity is 2000-.
Has the advantages that: the cyperus esculentus seeds are placed in a limited environment and are cultured by combining a culture medium, so that the germination rate is guaranteed, and the yield is improved.
According to the technical scheme, compared with the prior art, the method for germinating the cyperus esculentus seeds has the advantages that the germination rate is remarkably improved on the basis of keeping the quality of the cyperus esculentus, and the seed treatment method is simple and easy to implement and good in benefit. The EMS solution dosage and concentration limited by the invention are matched with the 6-BA solution to soak the germination rate to be more than 95 percent, partial repeated experiments can achieve 100 percent germination, and the generation of dead seedlings is reduced and the yield is further improved by the culture of the culture medium and the control of the culture conditions.
Detailed Description
The following will clearly and completely describe the technical solutions in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
The embodiment of the invention discloses a method for germinating cyperus esculentus seeds.
In the examples, the raw materials and reagents involved are purchased from conventional commercial channels, no requirement is made on brand sources, and the experimental methods not mentioned are conventional experimental methods, which are not described herein again.
Example 1
A method for germinating cyperus esculentus seeds comprises the following steps:
(1) pretreatment: cleaning cyperus esculentus seeds, and selecting full, non-pest, non-damaged and mature seeds A for later use;
(2) nutrient solution soaking treatment:
21) soaking the seeds A in a 6-BA solution with the concentration of 60mg/L for 1.5h, wherein the volume of the seeds is 2 times that of the seeds. Obtaining seeds B;
22) soaking the seeds B in purified water C for 15h, adding EMS solution into the purified water C to obtain mixed solution D, mixing and soaking, wherein the final concentration of the EMS solution is 0.2%, and the soaking time is 5.5 to obtain seeds E;
(3) culture in a culture medium: and washing the seeds E, and putting the seeds E in a germination culture medium for completing the culture.
Wherein, the germination culture medium comprises the following components: 15g/L sucrose, 100mg/LGA3And 8g/L agar.
The culture conditions were: illumination intensity 3000lx, humidity 55% at 25 ℃ for 2 d.
Example 2
A method for germinating cyperus esculentus seeds comprises the following steps:
(1) pretreatment: cleaning cyperus esculentus seeds, and selecting full, non-pest, non-damaged and mature seeds A for later use;
(2) nutrient solution soaking treatment:
21) and (3) soaking the seeds A in 6-BA solution with the concentration of 70mg/L for 1.2h, wherein the volume of the seeds is 2.5 times that of the seeds. Obtaining seeds B;
22) soaking the seeds B in purified water C for 16h, adding EMS solution into the purified water C to obtain mixed solution D, mixing and soaking, wherein the final concentration of the EMS solution is 0.2%, and soaking for 5 to obtain seeds E;
(3) culture in a culture medium: and washing the seeds E, and putting the seeds E in a germination culture medium for completing the culture.
Wherein, the germination culture medium comprises the following components: 13g/L sucrose, 120mg/LGA3And 9g/L agar.
The culture conditions were: at 27 deg.C, the illumination intensity is 2500lx, the humidity is 60%, and the time is 3 d.
Example 3
A method for germinating cyperus esculentus seeds comprises the following steps:
(1) pretreatment: cleaning cyperus esculentus seeds, and selecting full, non-pest, non-damaged and mature seeds A for later use;
(2) nutrient solution soaking treatment:
21) soaking the seeds A in 6-BA solution with the concentration of 80mg/L for 1h, wherein the volume of the seeds A is 3 times that of the seeds. Obtaining seeds B;
22) soaking the seeds B in purified water C for 18h, adding EMS solution into the purified water C to obtain mixed solution D, mixing and soaking, wherein the final concentration of the EMS solution is 0.2%, and the soaking time is 4.5 to obtain seeds E;
(3) culture in a culture medium: and washing the seeds E, and putting the seeds E in a germination culture medium for completing the culture.
Wherein, the germination culture medium comprises the following components: 12g/L sucrose, 150mg/LGA3And 10g/L agar.
The culture conditions were: at 30 ℃, the illumination intensity is 2000lx, the humidity is 65%, and the time is 4 d.
Comparative example 1
The difference from the example 2 is that the step of adding the EMS solution mixing soaking treatment in the step 22) is omitted, and the rest is the same as the example 2.
Comparative example 2
The difference from example 2 is that the culture medium culture and the limitation of culture conditions are omitted, wherein the culture medium in step (3) is replaced by soil mainly comprising humus, garden soil, a small amount of vermiculite and the like, and the culture is carried out in a natural environment for 3 d.
Comparative example 3
The difference from example 2 is that the final concentration of EMS solution is 0.6%, and the soaking time is 6h, which is the same as example 2.
Control experiment:
the methods provided in examples 1-3 and comparative examples 1-3 were used to treat cyperus esculentus seeds, respectively, and the seeds were planted in the experimental field (the steps of weeding, fertilizer, water, drug management, etc. were substantially identical in the later stage), and the results (averaged) are shown in table 1. Wherein, after the seed treatment in the step (3) is finished, the germination rate of the seed is counted; and (5) counting the dead seedling rate after the healthy buds are transplanted into the field for 5 days.
TABLE 1
The experimental result shows that the elimination of the EMS solution treatment step can obviously reduce the germination rate and influence the future yield; however, the EMS solution concentration is too high, the germination of the cyperus esculentus seeds is seriously influenced by too long soaking time, the yield is seriously influenced, the EMS solution dosage and the concentration limited by the method are matched with the 6-BA solution to soak the cyperus esculentus seeds, the germination rate can reach more than 95 percent (100 percent germination can be reached by partial repeated experiments), and the yield is relatively stable; omission of environmental control and medium culture steps slightly reduces germination rate, but increases the incidence of dead shoots.
The embodiments in the present description are described in a progressive manner, each embodiment focuses on differences from other embodiments, and the same and similar parts among the embodiments are referred to each other.
The previous description of the disclosed embodiments is provided to enable any person skilled in the art to make or use the present invention. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the generic principles defined herein may be applied to other embodiments without departing from the spirit or scope of the invention. Thus, the present invention is not intended to be limited to the embodiments shown herein but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.
Claims (1)
1. A method for germinating cyperus esculentus seeds is characterized by comprising the following steps:
(1) pretreatment: cleaning cyperus esculentus seeds, and selecting full, non-pest, non-damaged and mature seeds A for later use;
(2) nutrient solution soaking treatment:
21) soaking the seeds A in a 6-BA solution with the seed volume of 2-3 times to obtain seeds B;
22) soaking the seeds B in purified water C, adding EMS solution into the purified water C to obtain a mixed solution D, and mixing and soaking to obtain seeds E;
(3) culture in a culture medium: washing the seeds E, and placing the seeds E in a germination culture medium for germination;
in the step 21), the concentration of the 6-BA solution is 60-80 mg/L, and the soaking time is 1-1.5 h;
in the step 22), soaking the pure water C for 15-18 h;
in the step 22), the final concentration of the EMS solution in the mixed solution D is 0.2%, and the mixing and soaking time is 4.5-5.5 h;
in the step (3), the germination medium consists of the following components: 12-15 g/L sucrose, 100-150 mg/LGA3And 8-10 g/L agar;
the culture conditions in the step (3) are as follows: at 25-30 ℃, the illumination intensity is 2000-.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN109588316A (en) * | 2019-01-24 | 2019-04-09 | 中国科学院植物研究所 | A kind of method for tissue culture of cyperue esculentus |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN109588316A (en) * | 2019-01-24 | 2019-04-09 | 中国科学院植物研究所 | A kind of method for tissue culture of cyperue esculentus |
Non-Patent Citations (4)
| Title |
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| EMS诱变对油莎豆种子萌发特性的影响研究;何嘉欣等;《广东石油化工学院学报》;20191215;第29卷(第06期);第40-42，47页"摘要，第40-41页第1节" * |
| Establishment of rapid propagation system for Saponaria officinalis L. progeny after EMS mutation;Yang Xue et al.;《Journal of Northeast Agricultural University》;20121231;第19卷(第01期);第21-26页 * |
| 植物生长调节剂及不同温度处理对油莎豆块茎萌发的影响;沈雁等;《西南农业学报》;20101028;第23卷(第05期);第1464-1467页"第1464页右栏第1节，第1465页第2.3节，第1466页表3以及第3节" * |
| 油莎豆快速繁殖体系的研究;李佳婷等;《热带亚热带植物学报》;20190715;第27卷(第04期);第446-451页"第447页第1.1-1.2节，第2.1节，第448页图1" * |
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