CN111826333A - Culture method for treating prochloraz high-salt mixed wastewater strain - Google Patents
Culture method for treating prochloraz high-salt mixed wastewater strain Download PDFInfo
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- CN111826333A CN111826333A CN202010714471.4A CN202010714471A CN111826333A CN 111826333 A CN111826333 A CN 111826333A CN 202010714471 A CN202010714471 A CN 202010714471A CN 111826333 A CN111826333 A CN 111826333A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/36—Adaptation or attenuation of cells
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
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- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/02—Separating microorganisms from their culture media
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- C02F2101/38—Organic compounds containing nitrogen
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Abstract
The invention belongs to the technical field of chemical wastewater treatment, and particularly relates to a culture method for a strain for treating prochloraz high-salt mixed wastewater, which comprises the following steps: (1) collecting a sample; (2) performing amplification culture; (3) and (5) domesticating strains. The invention takes the active bottom mud of a prochloraz factory as a raw material, and obtains the strain suitable for treating the prochloraz high-salt mixed wastewater after aerobic acclimation treatment and screening. The method is used for treating the prochloraz high-salt mixed wastewater with the COD content of 20000-25000 mg/L, and the removal rate is more than 96%. The method can be used for directionally and stably screening, separating and culturing the strain capable of treating the prochloraz high-salt mixed wastewater, has low investment cost, obviously improves the microbial activity and improves the treatment efficiency of the prochloraz high-salt mixed wastewater.
Description
Technical Field
The invention belongs to the technical field of chemical wastewater treatment, and particularly relates to a culture method for a strain for treating prochloraz high-salt mixed wastewater.
Background
The prochloraz is N-propyl-N- [2- (2, 4, 6-trichlorophenoxy) ethyl-1H-imidazole-1-formamide, is a broad-spectrum bactericide, has obvious control effect on diseases of various crops caused by ascomycetes and imperfect fungi, and can also be mixed with most bactericides, insecticides and herbicides to achieve better control effect. It has therapeutic and eradicating effects on various diseases of field crops, fruits and vegetables, turf and ornamental plants. The bactericide is popularized and used in China at the end of the 20 th century, is widely applied to the production, storage, transportation and other processes of agricultural and forestry products such as grains, vegetables, fruits, flowers and the like at present, has obvious control effect, and is a bactericide which is widely applied in recent years.
The preparation method of prochloraz is multiple, and the process route is complicated. At present, domestic manufacturers adopt a production method of etherifying 2, 4, 6-trichlorophenol and dichloroethane in sodium hydroxide, then carrying out amination, acyl chlorination and condensation, the production processes are basically the same, and the process flow is as follows: taking 2, 4, 6-trichlorophenol as a starting material, adding liquid alkali to dissolve the trichlorophenol, and then carrying out pressure etherification on the trichlorophenol and dichloroethane; washing with water to remove dichloroethane; refluxing and aminating in propylamine, reinforcing alkali after aminating, steaming to remove propylamine, cooling, and adding hydrochloric acid to obtain secondary amine salt precipitate; filtering to separate insoluble substance, dehydrating the filter cake with toluene, and adding trichloromethyl carbonate (BTC) for acyl chlorination; then condensing with imidazole in the presence of triethylamine; washing the condensation liquid, refining, and removing toluene in an organic layer in vacuum to obtain the prochloraz original drug.
The prochloraz high-salt mixed wastewater comprises etherification, amination and condensation section wastewater and triethylamine wastewater, the wastewater of each section has the function of inhibiting or poisoning the growth of microorganisms, and the discharge of a large amount of standard wastewater can pollute the surrounding environment and a receiving water body and endanger the surrounding sea area, thereby causing serious threat and influence on human life and production.
The biological strengthening technology is characterized in that microorganisms with specific functions are added into a biological treatment system to improve the treatment effect of the original treatment system, and the added microorganisms can be derived from the original treatment system.
Researchers at home and abroad apply the technology to the treatment of the refractory toxic and harmful substances in industrial wastewater, surface water and underground water or improve the treatment effect of the wastewater, and can obviously improve the activity of microorganisms and improve the treatment efficiency of high-salinity wastewater.
The strain adopted for treating the prochloraz high-salt mixed wastewater by the biological method is an aerobic strain, the carbon source is toluene, and the nitrogen source is n-propylamine and triethylamine, so that the dominant strain for degrading the toluene, the n-propylamine and the triethylamine can be obtained, and the treatment efficiency is improved.
Disclosure of Invention
The invention aims to solve the defects of high investment cost and easy generation of secondary pollutants of a prochloraz high-salt mixed wastewater treatment method in the prior art, and provides a culture method for treating prochloraz high-salt mixed wastewater strains.
In order to achieve the purpose, the technical scheme adopted by the invention is as follows:
a culture method for treating a prochloraz high-salt mixed wastewater strain comprises the following steps:
(1) collecting samples: collecting active bottom mud of a prochloraz production plant by using an aseptic sampling bottle, and putting the active bottom mud into a thermostat for later use, wherein the temperature is 28-35 ℃;
(2) amplification culture: respectively adding toluene, n-propylamine and triethylamine into the active bottom mud treated in the step (1) under an aerobic condition, and performing stable culture at the temperature of 28-35 ℃ for 2-3 days;
wherein the adding amount of the toluene, the n-propylamine and the triethylamine is 6-10 g/L respectively;
(3) the strain domestication comprises the following steps:
a. mixing prochloraz high-salt mixed wastewater with COD of 10000-15000 mg/L with the active bottom mud treated in the step (2) to ensure that the concentration of the active bottom mud is 30000-35000 mg/L, blowing oxygen, controlling the temperature to be within the range of 30-35 ℃, adding an enrichment medium while stirring, acclimating by a shaking table at constant temperature for 10-15 days under aerobic conditions, stopping stirring, standing for 1-2 hours to settle the active bottom mud, and pouring out upper water and floating mud;
b. on the basis of the step a, adding prochloraz high-salt mixed wastewater with COD of 20000-25000 mg/L, supplementing active bottom mud, enabling the concentration of the active bottom mud to be 30000-35000 mg/L, blowing air, controlling the temperature to be within the range of 30-35 ℃, adding an enrichment medium while stirring, acclimating for 10-15 days at constant temperature by using a shaking table, stopping stirring, standing for 1-2 hours to enable the active bottom mud to settle, pouring out upper-layer water and floating mud, and taking the lower-layer active bottom mud as a prochloraz high-salt mixed wastewater strain.
Preferably, in the step (3), the enrichment medium consists of 15-16 g of beef extract, 1.5-2.5 g of monopotassium phosphate, 3-5 g of magnesium sulfate, 2-3 g of calcium chloride, 0.002-0.003 g of ferric sulfate and 0.2-0.3 g of urea.
Preferably, in the step (3) a, the addition amount of the enrichment medium is 0.05-2% of the total mass of the prochloraz high-salt mixed wastewater and the active bottom mud.
Preferably, in the step (3) b, the addition amount of the enrichment medium is 0.01-1% of the total mass of the prochloraz high-salt mixed wastewater and the active bottom mud.
Preferably, in the step (3), the pH value of the prochloraz high-salt mixed wastewater is adjusted to be 5.2-6.8.
Preferably, in the step (3) a, the dissolved oxygen amount of the prochloraz high-salt mixed wastewater is controlled within the range of 0.5-5.0 mg/L by blowing oxygen.
Preferably, in the step (3) b, the dissolved oxygen amount of the prochloraz high-salt mixed wastewater is controlled within the range of 0.3-2.0 mg/L by blowing air.
Preferably, the COD of the prochloraz high-salt mixed wastewater is: 20000-25000 mg/L, TDS: 99.6-99.9 g/L.
Compared with the prior art, the invention has the following advantages and positive effects:
the active bottom mud of a prochloraz production plant is taken as a raw material, and the strain suitable for treating the prochloraz high-salt mixed wastewater is obtained after aerobic acclimation treatment and screening. The method is used for treating the prochloraz high-salt mixed wastewater with the COD content of 20000-25000 mg/L, and the removal rate is more than 96%.
The method can be used for directionally and stably screening, separating and culturing the strain capable of treating the high-salt mixed prochloraz wastewater, has low investment cost, obviously improves the microbial activity and improves the treatment efficiency of the high-salt mixed prochloraz wastewater.
Detailed Description
The following is a detailed description of specific embodiments of the invention.
Examples
A culture method for treating a prochloraz high-salt mixed wastewater strain comprises the following steps:
(1) collecting samples: collecting active bottom mud of a prochloraz production plant by using an aseptic sampling bottle, marking the active bottom mud, sending the active bottom mud back to a laboratory, and putting the active bottom mud into a thermostat for later use, wherein the temperature is 28-35 ℃;
(2) amplification culture: oxygenating the collected sediment by using an aeration device, respectively adding toluene, n-propylamine and triethylamine into the active sediment treated in the step (1), and performing stable culture at the temperature of 28-35 ℃ for 2-3 days;
wherein the addition amount of toluene is 10g/L, the addition amount of n-propylamine is 6g/L, and the addition amount of triethylamine is 8 g/L;
(3) the strain domestication comprises the following steps:
a. mixing the prochloraz high-salt mixed wastewater with the COD of 12500mg/L with the active bottom mud treated in the step (2) to ensure that the concentration of the active bottom mud is 30000-35000 mg/L, blowing oxygen, controlling the temperature to be within the range of 30-35 ℃, adding an enrichment medium while stirring, acclimating by a shaking table at constant temperature for 10-15 days under aerobic conditions, stopping stirring, standing for 1-2 hours to settle the active bottom mud, and pouring out upper water and floating mud;
b. on the basis of the step a, adding prochloraz high-salt mixed wastewater with COD of 22270mg/L, supplementing active bottom mud to enable the concentration of the active bottom mud to be 30000-35000 mg/L, blowing air, controlling the temperature to be within the range of 30-35 ℃, adding an enrichment medium while stirring, acclimating for 10-15 days by using a shaking table at constant temperature, stopping stirring, standing for 1-2 hours to enable the active bottom mud to be settled, pouring out upper water and floating mud, and taking the lower active bottom mud as a prochloraz high-salt mixed wastewater strain.
And (3) the enrichment medium consists of 15.5g of beef extract, 2.0g of monopotassium phosphate, 4.0g of magnesium sulfate, 2.5g of calcium chloride, 0.0025g of ferric sulfate and 0.25g of urea.
In the step (3), the adding amount of the enrichment culture medium is 1.05 percent of the total mass of the prochloraz high-salt mixed wastewater and the active bottom mud.
In the step (3) b, the addition amount of the enrichment culture medium is 0.05 percent of the total mass of the prochloraz high-salt mixed wastewater and the active bottom mud.
And (3) adjusting the pH value of the prochloraz high-salt mixed wastewater to be 6.3.
And (3) a, controlling the dissolved oxygen amount of the prochloraz high-salt mixed wastewater to be within the range of 0.5-5.0 mg/L by blowing oxygen.
And (3) b, controlling the dissolved oxygen amount of the prochloraz high-salt mixed wastewater to be within the range of 0.3-2.0 mg/L by blowing air.
Verification example
500mL of prochloraz high-salt mixed wastewater with a COD value of 22270mg/L is put into a 1000mL reaction bottle provided with a mechanical stirring device, a thermometer, an oxygen aeration device and an air bubbling device, activated sludge obtained by domestication in the embodiment is added to enable the concentration of the activated sludge to reach 30000-35000 mg/L, the temperature in a water bath is controlled to be 30-35 ℃ under slow stirring, oxygen is blown in to enable the dissolved oxygen amount of the wastewater in the reaction bottle to be 3-4 mg/L. After 72 hours of reaction, sampling and detecting, the COD value in the waste water is reduced to 12000mg/L (the operation is aerobic operation). And (3) continuing the reaction, blowing air to ensure that the dissolved oxygen of the wastewater in the reaction bottle reaches 0.5-1.2 mg/L, and stirring and reacting for 48 hours at the temperature of 30-35 ℃. Water sample analysis is carried out, the COD of the wastewater is reduced to 850mg/L, and the removal rate of the COD reaches 96.2 percent (the above is an oxygen consumption process).
The above embodiments are merely preferred embodiments of the present invention, and any simple modification, modification and substitution changes made to the above embodiments according to the technical spirit of the present invention are within the scope of the technical solution of the present invention.
Claims (8)
1. A culture method for treating prochloraz high-salt mixed wastewater strains is characterized by comprising the following steps:
(1) collecting samples: collecting active bottom mud of a prochloraz production plant by using an aseptic sampling bottle, and putting the active bottom mud into a thermostat for later use, wherein the temperature is 28-35 ℃;
(2) amplification culture: respectively adding toluene, n-propylamine and triethylamine into the active bottom mud treated in the step (1) under an aerobic condition, and performing stable culture at the temperature of 28-35 ℃ for 2-3 days;
wherein the adding amount of the toluene, the n-propylamine and the triethylamine is 6-10 g/L respectively;
(3) the strain domestication comprises the following steps:
a. mixing prochloraz high-salt mixed wastewater with COD of 10000-15000 mg/L with the active bottom mud treated in the step (2) to ensure that the concentration of the active bottom mud is 30000-35000 mg/L, blowing oxygen, controlling the temperature to be within the range of 30-35 ℃, adding an enrichment medium while stirring, acclimating by a shaking table at constant temperature for 10-15 days under aerobic conditions, stopping stirring, standing for 1-2 hours to settle the active bottom mud, and pouring out upper water and floating mud;
b. on the basis of the step a, adding prochloraz high-salt mixed wastewater with COD of 20000-25000 mg/L, supplementing active bottom mud, enabling the concentration of the active bottom mud to be 30000-35000 mg/L, blowing air, controlling the temperature to be within the range of 30-35 ℃, adding an enrichment medium while stirring, acclimating for 10-15 days at constant temperature by using a shaking table, stopping stirring, standing for 1-2 hours to enable the active bottom mud to settle, pouring out upper-layer water and floating mud, and taking the lower-layer active bottom mud as a prochloraz high-salt mixed wastewater strain.
2. The culture method for treating the prochloraz high-salt mixed wastewater strain according to claim 1, wherein in the step (3), the enrichment medium comprises 15-16 g of beef extract, 1.5-2.5 g of monopotassium phosphate, 3-5 g of magnesium sulfate, 2-3 g of calcium chloride, 0.002-0.003 g of ferric sulfate and 0.2-0.3 g of urea.
3. The culture method for treating the prochloraz high-salt mixed wastewater strain according to claim 1, wherein in the step (3) a, the addition amount of the enrichment medium is 0.05-2% of the total mass of the prochloraz high-salt mixed wastewater and the active bottom mud.
4. The culture method for treating the prochloraz high-salt mixed wastewater strain according to claim 1, wherein in the step (3) b, the addition amount of the enrichment medium is 0.01-1% of the total mass of the prochloraz high-salt mixed wastewater and the active bottom mud.
5. The culture method for treating the bacterial spawn of the prochloraz high-salt mixed wastewater as claimed in claim 1, wherein in the step (3), the pH of the prochloraz high-salt mixed wastewater is adjusted to be 5.2-6.8.
6. The culture method of the bacterial strain for treating the prochloraz-high-salt mixed wastewater as claimed in claim 1, wherein in the step (3) a, the dissolved oxygen amount of the prochloraz-high-salt mixed wastewater is controlled to be within the range of 0.5-5.0 mg/L by blowing oxygen.
7. The culture method of the bacterial strain for treating the prochloraz-high-salt mixed wastewater as claimed in claim 1, wherein in the step (3) b, the dissolved oxygen amount of the prochloraz-high-salt mixed wastewater is controlled to be within the range of 0.3-2.0 mg/L by blowing air.
8. The culture method for treating the prochloraz-high-salt mixed wastewater strain according to claim 1, wherein the COD of the prochloraz-high-salt mixed wastewater is: 20000-25000 mg/L, TDS: 99.6-99.9 g/L.
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2003099010A1 (en) * | 2002-05-29 | 2003-12-04 | Basf Agro B.V., Arnhem(Nl), Wädenswil-Branch | Fungicidal seed treatment agent for oilseed rape |
| CN103173400A (en) * | 2013-03-04 | 2013-06-26 | 上海其新生物科技有限公司 | Culturing method of strain used for treating high-concentration tert-butyl alcohol wastewater |
| CN103898024A (en) * | 2014-04-03 | 2014-07-02 | 普罗生物技术（上海）有限公司 | Cultivation method of system volatile mixed organic waste gas strain for treating petrochemical wastewater |
| CN108892302A (en) * | 2018-07-23 | 2018-11-27 | 绍兴齐英膜科技有限公司 | The integrated conduct method of Prochloraz production waste water |
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- 2020-07-22 CN CN202010714471.4A patent/CN111826333B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2003099010A1 (en) * | 2002-05-29 | 2003-12-04 | Basf Agro B.V., Arnhem(Nl), Wädenswil-Branch | Fungicidal seed treatment agent for oilseed rape |
| CN103173400A (en) * | 2013-03-04 | 2013-06-26 | 上海其新生物科技有限公司 | Culturing method of strain used for treating high-concentration tert-butyl alcohol wastewater |
| CN103898024A (en) * | 2014-04-03 | 2014-07-02 | 普罗生物技术（上海）有限公司 | Cultivation method of system volatile mixed organic waste gas strain for treating petrochemical wastewater |
| CN108892302A (en) * | 2018-07-23 | 2018-11-27 | 绍兴齐英膜科技有限公司 | The integrated conduct method of Prochloraz production waste water |
Non-Patent Citations (2)
| Title |
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| 陈定盛等: "活性污泥法处理甲苯废气吸收液的研究" * |
| 马慧峰等: "高盐废水对好氧活性污泥工艺的影响分析" * |
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