CN111826333B - Culture method for treating prochloraz high-salt mixed wastewater strain - Google Patents
Culture method for treating prochloraz high-salt mixed wastewater strain Download PDFInfo
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- CN111826333B CN111826333B CN202010714471.4A CN202010714471A CN111826333B CN 111826333 B CN111826333 B CN 111826333B CN 202010714471 A CN202010714471 A CN 202010714471A CN 111826333 B CN111826333 B CN 111826333B
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- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
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Abstract
The invention belongs to the technical field of chemical wastewater treatment, and in particular relates to a culture method for treating prochloraz high-salt mixed wastewater strains, which comprises the following steps: (1) sample collection; (2) amplification culture; and (3) domesticating the strain. The invention takes active sediment of prochloraz production plants as raw materials, and obtains bacterial suitable for treating prochloraz high-salt mixed wastewater after aerobic domestication treatment and screening. The prochloraz high-salt mixed wastewater with the COD content of 20000-25000 mg/L is treated by the method, and the removal rate is more than 96%. The method can directionally, stably screen and separate the strains which can treat the prochloraz high-salt mixed wastewater, has low input cost, remarkably improves the microbial activity and improves the prochloraz high-salt mixed wastewater treatment efficiency.
Description
Technical Field
The invention belongs to the technical field of chemical wastewater treatment, and particularly relates to a culture method for treating prochloraz high-salt mixed wastewater strains.
Background
The prochloraz has the chemical name of N-propyl-N- [2- (2, 4, 6-trichlorophenoxy) ethyl-1H-imidazole-1-carboxamide, is a broad-spectrum bactericide, has obvious prevention effect on diseases caused by ascomycetes and half-known bacteria of various crops, and can also be mixed with most bactericides, pesticides and herbicides, and has good prevention and treatment effects. Has the functions of treating and eradicating various diseases on field crops, fruits and vegetables, turf and ornamental plants. The 20 th century is popularized and used in China, is widely applied to the production, storage, transportation and other processes of agriculture and forestry products such as grains, vegetables, fruits, flowers and the like, has remarkable prevention effect, and is a bactericide widely applied in recent years.
The prochloraz has a plurality of preparation methods and a great deal of process routes. At present, domestic manufacturers adopt production methods of etherification, amination, acyl chlorination and condensation of 2,4, 6-trichlorophenol and dichloroethane in sodium hydroxide, the production processes are basically the same, and the process flow is as follows: 2,4, 6-trichlorophenol is taken as a starting material, firstly added with liquid alkali for dissolution, and then pressurized and etherified with dichloroethane; removing dichloroethane after washing; then reflux amination is carried out in propylamine, then alkali is reinforced for evaporating propylamine after amination, and hydrochloric acid is added for cooling to obtain secondary amine salt precipitate; separating insoluble substances by suction filtration, dehydrating a filter cake by toluene, and adding trichloromethyl carbonate (BTC) for acyl chlorination; then condensing with imidazole in the presence of triethylamine; the condensed liquid is washed and refined, toluene is removed from the organic layer in vacuum, and prochloraz technical is obtained.
The prochloraz high-salt mixed wastewater comprises etherification, amination and condensation section wastewater and triethylamine wastewater, the section wastewater has inhibition or toxic action on microorganism growth, and the discharge of a large amount of standard wastewater can pollute the surrounding environment and the receiving water body, endanger the surrounding sea area and further cause serious threat and influence on human life and production.
The biological strengthening technology belongs to biological treatment technology, and the biological strengthening technology refers to adding microorganisms with specific functions into a biological treatment system to improve the treatment effect of the original treatment system, wherein the added microorganisms can be derived from the original treatment system.
Researchers at home and abroad have applied the technology to the treatment of difficult-to-degrade toxic and harmful substances in industrial wastewater, surface water and underground water or to improve the wastewater treatment effect, can obviously improve the microbial activity and improve the high-salt wastewater treatment efficiency.
The bacteria used for treating the prochloraz high-salt mixed wastewater by the biological method are aerobic bacteria, toluene is selected as a carbon source, n-propylamine and triethylamine are selected as a nitrogen source, so that the dominant bacteria for degrading the toluene, the n-propylamine and the triethylamine can be obtained, and the treatment efficiency is improved.
Disclosure of Invention
The invention aims to solve the defects of high investment cost and easiness in generating secondary pollutants of a prochloraz high-salt mixed wastewater treatment method in the prior art, and provides a culture method for treating prochloraz high-salt mixed wastewater strains.
In order to achieve the above purpose, the invention adopts the following technical scheme:
a culture method for treating prochloraz high-salt mixed wastewater strains comprises the following steps:
(1) Sample collection: collecting active sediment of prochloraz production plants by using a sterile sampling bottle, and placing the active sediment into an incubator for standby at the temperature of 28-35 ℃;
(2) Amplification culture: under the aerobic condition, toluene, n-propylamine and triethylamine are respectively added into the active sediment treated in the step (1) for constant culture at 28-35 ℃ for 2-3 days;
wherein, the addition amount of toluene, n-propylamine and triethylamine is 6-10 g/L respectively;
(3) The strain domestication method comprises the following steps:
a. mixing the prochloraz high-salt mixed wastewater with the COD of 10000-15000 mg/L with the active sediment treated in the step (2), leading the concentration of the active sediment to be 30000-35000 mg/L, bubbling oxygen, controlling the temperature to be 30-35 ℃, stirring, adding an enrichment culture medium, carrying out shaking table constant temperature domestication for 10-15 days under an aerobic condition, stopping stirring, standing for 1-2 h to enable the active sediment to be settled, and pouring out supernatant water and floating mud;
b. on the basis of the step a, adding prochloraz high-salt mixed wastewater with COD of 20000-25000 mg/L, supplementing active sediment, leading the concentration of the active sediment to be 30000-35000 mg/L, bubbling air, controlling the temperature to be 30-35 ℃, stirring, adding enrichment culture medium, carrying out constant-temperature domestication on a shaking table for 10-15 days, stopping stirring, standing for 1-2 h to enable the active sediment to settle, pouring out upper water and floating mud, and leading the lower active sediment to be prochloraz high-salt mixed wastewater strain.
Preferably, in the step (3), the enrichment medium consists of 15-16 g of beef extract, 1.5-2.5 g of monopotassium phosphate, 3-5 g of magnesium sulfate, 2-3 g of calcium chloride, 0.002-0.003 g of ferric sulfate and 0.2-0.3 g of urea.
Preferably, in the step (3) a, the addition amount of the enrichment medium is 0.05-2% of the total mass of the prochloraz high-salt mixed wastewater and the active sediment.
Preferably, in the step (3) b, the addition amount of the enrichment medium is 0.01-1% of the total mass of the prochloraz high-salt mixed wastewater and the active sediment.
Preferably, in the step (3), the pH value of the prochloraz high-salt mixed wastewater is adjusted to be 5.2-6.8.
Preferably, in the step (3) a, oxygen is blown to control the dissolved oxygen amount of the prochloraz high-salt mixed wastewater to be in the range of 0.5-5.0 mg/L.
Preferably, in the step (3) b, the dissolved oxygen amount of the prochloraz high-salt mixed wastewater is controlled to be in the range of 0.3-2.0 mg/L by blowing air.
Preferably, the prochloraz high-salt mixed wastewater has a COD: 20000-25000 mg/L, TDS: 99.6-99.9 g/L.
Compared with the prior art, the invention has the following advantages and positive effects:
the active sediment of the prochloraz production plant is used as a raw material, and after aerobic domestication treatment and screening, the strain suitable for treating prochloraz high-salt mixed wastewater is obtained. The prochloraz high-salt mixed wastewater with the COD content of 20000-25000 mg/L is treated by the method, and the removal rate is more than 96%.
The method can directionally, stably screen and separate the strains capable of treating the prochloraz high-salt mixed wastewater, has low input cost, remarkably improves the microbial activity and improves the prochloraz high-salt mixed wastewater treatment efficiency.
Detailed Description
Specific embodiments of the present invention are described in detail below.
Examples
A culture method for treating prochloraz high-salt mixed wastewater strains comprises the following steps:
(1) Sample collection: collecting active sediment of prochloraz production plants by using a sterile sampling bottle, taking the active sediment as a mark, sending the mark back to a laboratory, and placing the laboratory in an incubator for standby at 28-35 ℃;
(2) Amplification culture: oxygenating the collected sediment by an aerator, and respectively adding toluene, n-propylamine and triethylamine into the active sediment treated in the step (1) to perform constant culture at 28-35 ℃ for 2-3 days;
wherein, the addition amount of toluene is 10g/L, the addition amount of n-propylamine is 6g/L, and the addition amount of triethylamine is 8g/L;
(3) The strain domestication method comprises the following steps:
a. mixing the prochloraz high-salt mixed wastewater with the COD of 12500mg/L with the active sediment treated in the step (2), leading the concentration of the active sediment to be 30000-35000 mg/L, bubbling oxygen, controlling the temperature to be 30-35 ℃, stirring, adding an enrichment culture medium, carrying out constant-temperature domestication on a shaking table for 10-15 days under an aerobic condition, stopping stirring, standing for 1-2 h to enable the active sediment to settle, and pouring out upper water and floating mud;
b. on the basis of the step a, adding the prochloraz high-salt mixed wastewater with COD of 22270mg/L, supplementing active sediment, leading the concentration of the active sediment to be 30000-35000 mg/L, bubbling air, controlling the temperature to be 30-35 ℃, stirring, adding an enrichment culture medium, carrying out constant-temperature domestication on a shaking table for 10-15 days, stopping stirring, standing for 1-2 hours to enable the active sediment to settle, pouring out upper water and floating mud, and leading the lower active sediment to be prochloraz high-salt mixed wastewater strains.
The enrichment medium in the step (3) consists of 15.5g of beef extract, 2.0g of monopotassium phosphate, 4.0g of magnesium sulfate, 2.5g of calcium chloride, 0.0025g of ferric sulfate and 0.25g of urea.
And (3) a, wherein the addition amount of the enrichment medium is 1.05% of the total mass of the prochloraz high-salt mixed wastewater and the active sediment.
And (3) b, wherein the addition amount of the enrichment medium is 0.05% of the total mass of the prochloraz high-salt mixed wastewater and the active sediment.
And (3) regulating the pH value of the prochloraz high-salt mixed wastewater to 6.3.
And (3) a, controlling the dissolved oxygen amount of the prochloraz high-salt mixed wastewater to be in the range of 0.5-5.0 mg/L by blowing oxygen.
And (3) b, controlling the dissolved oxygen content of the prochloraz high-salt mixed wastewater within the range of 0.3-2.0 mg/L by blowing air.
Verification example
500mL of prochloraz high-salt mixed wastewater with the COD value of 22270mg/L is put into a 1000mL reaction bottle provided with a mechanical stirring device, a thermometer, an oxygen aeration device and an air bubbling device, activated sludge obtained by domestication in the embodiment is added to enable the concentration of the activated sludge to reach 30000-35000 mg/L, and under slow stirring, the internal temperature of a water bath is controlled at 30-35 ℃ and oxygen is blown in to enable the dissolved oxygen of the wastewater in the reaction bottle to be 3-4 mg/L. After 72 hours of reaction, sampling and detection are carried out, and the COD value in the wastewater is reduced to 12000mg/L (the above operation is aerobic operation). Continuing the reaction, blowing air to make the dissolved oxygen content of the wastewater in the reaction bottle reach 0.5-1.2 mg/L, and stirring and reacting for 48 hours at 30-35 ℃. And (3) analyzing the water sample, wherein the COD of the wastewater is reduced to 850mg/L, and the COD removal rate is 96.2% (the oxygen consumption process is the above).
The above embodiments are merely preferred embodiments of the present invention, and any simple modification, modification and substitution changes made to the above embodiments according to the technical substance of the present invention are all within the scope of the technical solution of the present invention.
Claims (5)
1. The culture method for treating the prochloraz high-salt mixed wastewater strain is characterized by comprising the following steps of:
(1) Sample collection: collecting active sediment of a prochloraz production plant by using a sterile sampling bottle, and placing the active sediment into an incubator for standby at 28-35 ℃;
(2) Amplification culture: under an aerobic condition, toluene, n-propylamine and triethylamine are respectively added into the active sediment treated in the step (1), and the constant-temperature culture is carried out for 2-3 days at the temperature of 28-35 ℃;
wherein the addition amount of toluene, n-propylamine and triethylamine is 6-10 g/L respectively;
(3) The strain domestication method comprises the following steps:
a. mixing high-salt prochloraz mixed wastewater with the COD of 10000-15000 mg/L with the active sediment treated in the step (2), so that the concentration of the active sediment is 30000-35000 mg/L, blowing oxygen, controlling the dissolved oxygen content of the prochloraz high-salt mixed wastewater to be 0.5-5.0 mg/L by blowing oxygen, controlling the temperature to be 30-35 ℃, stirring, adding an enrichment culture medium, wherein the enrichment culture medium consists of 15-16 g of beef extract, 1.5-2.5 g of monopotassium phosphate, 3-5 g of magnesium sulfate, 2-3 g of calcium chloride, 0.002-0.003g of ferric sulfate and 0.2-0.3 g of urea, carrying out constant-temperature domestication for 10-15 days under aerobic conditions, stopping stirring, standing for 1-2 h to enable the active sediment to settle, and pouring out upper water and floating mud;
b. on the basis of the step a, adding prochloraz high-salt mixed wastewater with COD of 20000-25000 mg/L, supplementing active sediment, enabling the concentration of the active sediment to be 30000-35000 mg/L, blowing air, controlling the dissolved oxygen of the prochloraz high-salt mixed wastewater to be in the range of 0.3-2.0 mg/L by blowing air, controlling the temperature to be in the range of 30-35 ℃, stirring, adding an enrichment culture medium, carrying out constant-temperature domestication on a shaking table for 10-15 days, stopping stirring, standing for 1-2 h to enable the active sediment to settle, pouring out supernatant water and floating mud, and enabling the lower active sediment to be prochloraz high-salt mixed wastewater strains.
2. The culture method for treating prochloraz high-salt mixed wastewater strains according to claim 1, wherein in the step (3) a, the addition amount of an enrichment medium is 0.05-2% of the total mass of prochloraz high-salt mixed wastewater and active sediment.
3. The culture method for treating prochloraz high-salt mixed wastewater strains according to claim 1, wherein in the step (3) b, the addition amount of the enrichment medium is 0.01-1% of the total mass of prochloraz high-salt mixed wastewater and active sediment.
4. The method for culturing the bacterial strain for treating the prochloraz high-salt mixed wastewater according to claim 1, wherein the pH of the prochloraz high-salt mixed wastewater in the step (3) is adjusted to be 5.2-6.8.
5. The method for culturing a strain for treating prochloraz high-salt mixed wastewater according to claim 1, wherein the prochloraz high-salt mixed wastewater has a COD: 20000-25000 mg/L, TDS: 99.6-99.9 g/L.
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2003099010A1 (en) * | 2002-05-29 | 2003-12-04 | Basf Agro B.V., Arnhem(Nl), Wädenswil-Branch | Fungicidal seed treatment agent for oilseed rape |
| CN103173400A (en) * | 2013-03-04 | 2013-06-26 | 上海其新生物科技有限公司 | Culturing method of strain used for treating high-concentration tert-butyl alcohol wastewater |
| CN103898024A (en) * | 2014-04-03 | 2014-07-02 | 普罗生物技术（上海）有限公司 | Cultivation method of system volatile mixed organic waste gas strain for treating petrochemical wastewater |
| CN108892302A (en) * | 2018-07-23 | 2018-11-27 | 绍兴齐英膜科技有限公司 | The integrated conduct method of Prochloraz production waste water |
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Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2003099010A1 (en) * | 2002-05-29 | 2003-12-04 | Basf Agro B.V., Arnhem(Nl), Wädenswil-Branch | Fungicidal seed treatment agent for oilseed rape |
| CN103173400A (en) * | 2013-03-04 | 2013-06-26 | 上海其新生物科技有限公司 | Culturing method of strain used for treating high-concentration tert-butyl alcohol wastewater |
| CN103898024A (en) * | 2014-04-03 | 2014-07-02 | 普罗生物技术（上海）有限公司 | Cultivation method of system volatile mixed organic waste gas strain for treating petrochemical wastewater |
| CN108892302A (en) * | 2018-07-23 | 2018-11-27 | 绍兴齐英膜科技有限公司 | The integrated conduct method of Prochloraz production waste water |
Non-Patent Citations (1)
| Title |
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| 马慧峰等.高盐废水对好氧活性污泥工艺的影响分析.《工业用水与废水》.2019,第50卷(第4期),49-51和69页. * |
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