CN111801568A - 测定血小板浓度的方法及系统 - Google Patents
测定血小板浓度的方法及系统 Download PDFInfo
- Publication number
- CN111801568A CN111801568A CN201980011879.0A CN201980011879A CN111801568A CN 111801568 A CN111801568 A CN 111801568A CN 201980011879 A CN201980011879 A CN 201980011879A CN 111801568 A CN111801568 A CN 111801568A
- Authority
- CN
- China
- Prior art keywords
- platelet
- suspension
- histogram
- blood sample
- distribution
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000000034 method Methods 0.000 title claims abstract description 169
- 210000001772 blood platelet Anatomy 0.000 claims abstract description 866
- 239000000725 suspension Substances 0.000 claims abstract description 326
- 210000004369 blood Anatomy 0.000 claims abstract description 320
- 239000008280 blood Substances 0.000 claims abstract description 320
- 238000009826 distribution Methods 0.000 claims abstract description 156
- 210000000265 leukocyte Anatomy 0.000 claims abstract description 110
- 210000003743 erythrocyte Anatomy 0.000 claims abstract description 109
- 230000003287 optical effect Effects 0.000 claims abstract description 86
- 238000000149 argon plasma sintering Methods 0.000 claims abstract description 48
- 238000004159 blood analysis Methods 0.000 claims abstract description 48
- 210000004027 cell Anatomy 0.000 claims abstract description 38
- 238000004458 analytical method Methods 0.000 claims abstract description 24
- 239000003085 diluting agent Substances 0.000 claims abstract description 23
- 239000007850 fluorescent dye Substances 0.000 claims abstract description 23
- 238000003860 storage Methods 0.000 claims abstract description 13
- 239000003219 hemolytic agent Substances 0.000 claims abstract description 10
- 238000001514 detection method Methods 0.000 claims description 76
- 238000000926 separation method Methods 0.000 claims description 55
- 238000012545 processing Methods 0.000 claims description 35
- 239000003153 chemical reaction reagent Substances 0.000 claims description 20
- 238000004883 computer application Methods 0.000 claims description 17
- 239000003795 chemical substances by application Substances 0.000 claims description 14
- 210000003979 eosinophil Anatomy 0.000 claims description 14
- 210000004698 lymphocyte Anatomy 0.000 claims description 14
- 210000001616 monocyte Anatomy 0.000 claims description 14
- 210000000440 neutrophil Anatomy 0.000 claims description 14
- 230000002949 hemolytic effect Effects 0.000 claims description 12
- 230000002934 lysing effect Effects 0.000 claims description 12
- 210000003651 basophil Anatomy 0.000 claims description 9
- 238000010186 staining Methods 0.000 claims description 9
- 238000004891 communication Methods 0.000 claims description 6
- 238000010586 diagram Methods 0.000 claims description 5
- 210000003924 normoblast Anatomy 0.000 claims description 2
- 238000004820 blood count Methods 0.000 claims 6
- 239000000523 sample Substances 0.000 description 176
- 238000007430 reference method Methods 0.000 description 35
- 230000004927 fusion Effects 0.000 description 25
- 230000002159 abnormal effect Effects 0.000 description 21
- 210000000601 blood cell Anatomy 0.000 description 16
- 238000002847 impedance measurement Methods 0.000 description 13
- 230000008569 process Effects 0.000 description 13
- 230000000875 corresponding effect Effects 0.000 description 10
- 239000000126 substance Substances 0.000 description 9
- -1 alkali metal salts Chemical class 0.000 description 8
- 238000005259 measurement Methods 0.000 description 8
- 239000002245 particle Substances 0.000 description 8
- 239000004065 semiconductor Substances 0.000 description 8
- 239000007864 aqueous solution Substances 0.000 description 7
- 239000003093 cationic surfactant Substances 0.000 description 6
- 239000000975 dye Substances 0.000 description 6
- 230000006870 function Effects 0.000 description 6
- 230000002101 lytic effect Effects 0.000 description 6
- 150000007523 nucleic acids Chemical class 0.000 description 6
- 102000039446 nucleic acids Human genes 0.000 description 6
- 108020004707 nucleic acids Proteins 0.000 description 6
- 230000015654 memory Effects 0.000 description 5
- 238000012360 testing method Methods 0.000 description 5
- 150000001449 anionic compounds Chemical class 0.000 description 4
- 238000000684 flow cytometry Methods 0.000 description 4
- 239000012634 fragment Substances 0.000 description 4
- 230000002452 interceptive effect Effects 0.000 description 4
- 239000002280 amphoteric surfactant Substances 0.000 description 3
- 125000000129 anionic group Chemical group 0.000 description 3
- 125000002091 cationic group Chemical group 0.000 description 3
- 230000002596 correlated effect Effects 0.000 description 3
- 230000004069 differentiation Effects 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 239000004094 surface-active agent Substances 0.000 description 3
- 206010018910 Haemolysis Diseases 0.000 description 2
- 239000003945 anionic surfactant Substances 0.000 description 2
- 230000009089 cytolysis Effects 0.000 description 2
- 238000010790 dilution Methods 0.000 description 2
- 239000012895 dilution Substances 0.000 description 2
- 238000009472 formulation Methods 0.000 description 2
- 230000008588 hemolysis Effects 0.000 description 2
- 238000000338 in vitro Methods 0.000 description 2
- 238000001698 laser desorption ionisation Methods 0.000 description 2
- 238000012417 linear regression Methods 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000000569 multi-angle light scattering Methods 0.000 description 2
- 239000002736 nonionic surfactant Substances 0.000 description 2
- 239000012192 staining solution Substances 0.000 description 2
- 238000010998 test method Methods 0.000 description 2
- 206010027540 Microcytosis Diseases 0.000 description 1
- 229910052783 alkali metal Inorganic materials 0.000 description 1
- 230000000740 bleeding effect Effects 0.000 description 1
- 239000007853 buffer solution Substances 0.000 description 1
- 210000000170 cell membrane Anatomy 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- 238000012937 correction Methods 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 230000023077 detection of light stimulus Effects 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 238000004090 dissolution Methods 0.000 description 1
- 238000001917 fluorescence detection Methods 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 238000004599 local-density approximation Methods 0.000 description 1
- 210000002652 macrocyte Anatomy 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 230000000877 morphologic effect Effects 0.000 description 1
- 150000002894 organic compounds Chemical class 0.000 description 1
- 239000011148 porous material Substances 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 239000011164 primary particle Substances 0.000 description 1
- 230000000630 rising effect Effects 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 210000002325 somatostatin-secreting cell Anatomy 0.000 description 1
Images
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/483—Physical analysis of biological material
- G01N33/487—Physical analysis of biological material of liquid biological material
- G01N33/49—Blood
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/06—Investigating concentration of particle suspensions
- G01N15/0656—Investigating concentration of particle suspensions using electric, e.g. electrostatic methods or magnetic methods
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/1031—Investigating individual particles by measuring electrical or magnetic effects
- G01N15/12—Investigating individual particles by measuring electrical or magnetic effects by observing changes in resistance or impedance across apertures when traversed by individual particles, e.g. by using the Coulter principle
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Optical investigation techniques, e.g. flow cytometry
- G01N15/1456—Optical investigation techniques, e.g. flow cytometry without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals
- G01N15/1459—Optical investigation techniques, e.g. flow cytometry without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals the analysis being performed on a sample stream
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/483—Physical analysis of biological material
- G01N33/487—Physical analysis of biological material of liquid biological material
- G01N33/48707—Physical analysis of biological material of liquid biological material by electrical means
-
- G—PHYSICS
- G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
- G16H—HEALTHCARE INFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR THE HANDLING OR PROCESSING OF MEDICAL OR HEALTHCARE DATA
- G16H10/00—ICT specially adapted for the handling or processing of patient-related medical or healthcare data
- G16H10/40—ICT specially adapted for the handling or processing of patient-related medical or healthcare data for data related to laboratory analysis, e.g. patient specimen analysis
-
- G—PHYSICS
- G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
- G16H—HEALTHCARE INFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR THE HANDLING OR PROCESSING OF MEDICAL OR HEALTHCARE DATA
- G16H15/00—ICT specially adapted for medical reports, e.g. generation or transmission thereof
-
- G—PHYSICS
- G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
- G16H—HEALTHCARE INFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR THE HANDLING OR PROCESSING OF MEDICAL OR HEALTHCARE DATA
- G16H40/00—ICT specially adapted for the management or administration of healthcare resources or facilities; ICT specially adapted for the management or operation of medical equipment or devices
- G16H40/60—ICT specially adapted for the management or administration of healthcare resources or facilities; ICT specially adapted for the management or operation of medical equipment or devices for the operation of medical equipment or devices
- G16H40/63—ICT specially adapted for the management or administration of healthcare resources or facilities; ICT specially adapted for the management or operation of medical equipment or devices for the operation of medical equipment or devices for local operation
-
- G—PHYSICS
- G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
- G16H—HEALTHCARE INFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR THE HANDLING OR PROCESSING OF MEDICAL OR HEALTHCARE DATA
- G16H40/00—ICT specially adapted for the management or administration of healthcare resources or facilities; ICT specially adapted for the management or operation of medical equipment or devices
- G16H40/60—ICT specially adapted for the management or administration of healthcare resources or facilities; ICT specially adapted for the management or operation of medical equipment or devices for the operation of medical equipment or devices
- G16H40/67—ICT specially adapted for the management or administration of healthcare resources or facilities; ICT specially adapted for the management or operation of medical equipment or devices for the operation of medical equipment or devices for remote operation
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/06—Investigating concentration of particle suspensions
- G01N15/075—Investigating concentration of particle suspensions by optical means
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/01—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials specially adapted for biological cells, e.g. blood cells
- G01N2015/016—White blood cells
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/01—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials specially adapted for biological cells, e.g. blood cells
- G01N2015/018—Platelets
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N2015/1006—Investigating individual particles for cytology
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N2015/1019—Associating Coulter-counter and optical flow cytometer [OFC]
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Optical investigation techniques, e.g. flow cytometry
- G01N2015/1486—Counting the particles
Landscapes
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Pathology (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Biochemistry (AREA)
- Analytical Chemistry (AREA)
- Hematology (AREA)
- Primary Health Care (AREA)
- Public Health (AREA)
- Epidemiology (AREA)
- Medical Informatics (AREA)
- Molecular Biology (AREA)
- Biophysics (AREA)
- Urology & Nephrology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Dispersion Chemistry (AREA)
- General Business, Economics & Management (AREA)
- Business, Economics & Management (AREA)
- Ecology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Investigating Or Analyzing Materials By The Use Of Electric Means (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
Abstract
一种分析血液样本中血小板浓度的方法、血液分析系统(100)及存储介质，测定血液样本中血小板浓度的方法包括：将血液样本的第一份与稀释液混合，形成第一悬浮液；将血液样本的第二份与溶血剂和荧光染料混合以溶解红细胞和染色白细胞，形成第二悬浮液；测量第一悬浮液流过小孔的直流阻抗信号；测量第二悬浮液流过光学流动室(20)的光散射信号和荧光信号；分析第一悬浮液的该直流阻抗信号以获取第一血小板分布；分析第二悬浮液的光散射信号和荧光信号以区分血小板和白细胞并获取第二血小板分布；基于第一血小板分布和第二血小板分布确定血液样本的血小板分析数据，如血小板浓度。
Description
PCT国内申请，说明书已公开。
Claims (69)
- PCT国内申请，权利要求书已公开。
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNPCT/CN2018/085197 | 2018-04-28 | ||
| CN2018085197 | 2018-04-28 | ||
| PCT/CN2019/084687 WO2019206313A1 (zh) | 2018-04-28 | 2019-04-26 | 测定血小板浓度的方法及系统 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN111801568A true CN111801568A (zh) | 2020-10-20 |
| CN111801568B CN111801568B (zh) | 2024-05-24 |
Family
ID=68294401
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201980011879.0A Active CN111801568B (zh) | 2018-04-28 | 2019-04-26 | 测定血小板浓度的方法及系统 |
Country Status (4)
| Country | Link |
|---|---|
| US (1) | US11841358B2 (zh) |
| EP (1) | EP3789751B1 (zh) |
| CN (1) | CN111801568B (zh) |
| WO (1) | WO2019206313A1 (zh) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114047109A (zh) * | 2022-01-11 | 2022-02-15 | 深圳市帝迈生物技术有限公司 | 一种样本分析仪及其计数方法 |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111337392A (zh) * | 2020-02-28 | 2020-06-26 | 清华大学深圳国际研究生院 | 一种悬浮颗粒偏振荧光同步测量装置 |
| WO2023010447A1 (zh) * | 2021-08-05 | 2023-02-09 | 深圳迈瑞动物医疗科技股份有限公司 | 一种样本分析装置、动物用分析装置和样本分析方法 |
| CN116952805A (zh) | 2022-04-14 | 2023-10-27 | 深圳迈瑞生物医疗电子股份有限公司 | 样本分析仪以及血小板计数方法 |
Citations (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5874310A (en) * | 1997-11-21 | 1999-02-23 | Coulter International Corp. | Method for differentiation of nucleated red blood cells |
| CN1323395A (zh) * | 1998-10-20 | 2001-11-21 | 库尔特国际公司 | 鉴别网状细胞的试剂组合物和方法 |
| US6525807B1 (en) * | 1999-01-21 | 2003-02-25 | Sysmex Corporation | Particle analyzing apparatus |
| US20040038413A1 (en) * | 2002-08-23 | 2004-02-26 | Kramer Donald L. | Method and apparatus for differentiating blood cells using back-scatter |
| US20060160229A1 (en) * | 2004-02-10 | 2006-07-20 | Beckman Coulter, Inc. | Method of measurement of nucleated red blood cells |
| CN103472034A (zh) * | 2013-08-23 | 2013-12-25 | 深圳中科强华科技有限公司 | 一种血细胞分析芯片、分析仪及分析方法 |
| CN103472216A (zh) * | 2013-08-23 | 2013-12-25 | 深圳中科强华科技有限公司 | 一种血细胞分析芯片、分析仪及分析方法 |
| CN103491771A (zh) * | 2011-03-17 | 2014-01-01 | 格雷莱格技术有限责任公司 | 用于自体生物疗法的系统和方法 |
| US20140186874A1 (en) * | 2012-12-31 | 2014-07-03 | Beckman Coulter, Inc. | Immature platelet enumeration systems and methods |
| CN104541149A (zh) * | 2012-07-05 | 2015-04-22 | 贝克曼考尔特公司 | 用于确定白血细胞计数的方法和装置 |
| CN106018771A (zh) * | 2015-03-27 | 2016-10-12 | 希森美康株式会社 | 血液分析装置及血液分析方法 |
| EP3258274A1 (en) * | 2016-06-17 | 2017-12-20 | Sysmex Corporation | Sample analyzer using a patient-specific detection history |
Family Cites Families (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US2656508A (en) | 1949-08-27 | 1953-10-20 | Wallace H Coulter | Means for counting particles suspended in a fluid |
| US3810011A (en) | 1970-05-25 | 1974-05-07 | Coulter Electronics | Apparatus and method for analyzing the particle volume distribution for a plurality of particles of different size in a quantity of liquid |
| JP3707620B2 (ja) * | 1993-05-14 | 2005-10-19 | コールター インターナショナル コーポレイション | 光散乱技術を使用した網赤血球分析方法と装置 |
| EP1412740B1 (en) * | 2001-07-27 | 2015-07-08 | Beckman Coulter, Inc. | Method for measurement of nucleated red blood cells |
| US7344890B2 (en) * | 2005-11-09 | 2008-03-18 | Beckman Coulter, Inc. | Method for discriminating platelets from red blood cells |
| CN101078721B (zh) | 2006-05-23 | 2010-12-22 | 深圳迈瑞生物医疗电子股份有限公司 | 对白细胞进行分类的试剂及方法 |
| CN101750274B (zh) | 2008-12-17 | 2014-06-25 | 深圳迈瑞生物医疗电子股份有限公司 | 白细胞分类计数试剂、试剂盒以及白细胞分类计数的方法 |
| CN102115456B (zh) | 2009-12-30 | 2014-08-20 | 深圳迈瑞生物医疗电子股份有限公司 | 花菁类化合物，包含所述化合物的组合物及其在细胞检测中的用途 |
| CN103471980B (zh) * | 2013-08-23 | 2015-08-26 | 深圳中科强华科技有限公司 | 一种芯片式血细胞分析装置及方法 |
| CN103471982B (zh) * | 2013-08-23 | 2015-11-25 | 深圳中科强华科技有限公司 | 一种血细胞分析芯片、分析仪及分析方法 |
-
2019
- 2019-04-26 CN CN201980011879.0A patent/CN111801568B/zh active Active
- 2019-04-26 WO PCT/CN2019/084687 patent/WO2019206313A1/zh unknown
- 2019-04-26 EP EP19792132.3A patent/EP3789751B1/en active Active
-
2020
- 2020-10-20 US US17/075,603 patent/US11841358B2/en active Active
Patent Citations (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5874310A (en) * | 1997-11-21 | 1999-02-23 | Coulter International Corp. | Method for differentiation of nucleated red blood cells |
| CN1323395A (zh) * | 1998-10-20 | 2001-11-21 | 库尔特国际公司 | 鉴别网状细胞的试剂组合物和方法 |
| US6525807B1 (en) * | 1999-01-21 | 2003-02-25 | Sysmex Corporation | Particle analyzing apparatus |
| US20040038413A1 (en) * | 2002-08-23 | 2004-02-26 | Kramer Donald L. | Method and apparatus for differentiating blood cells using back-scatter |
| US20060160229A1 (en) * | 2004-02-10 | 2006-07-20 | Beckman Coulter, Inc. | Method of measurement of nucleated red blood cells |
| CN103491771A (zh) * | 2011-03-17 | 2014-01-01 | 格雷莱格技术有限责任公司 | 用于自体生物疗法的系统和方法 |
| CN104541149A (zh) * | 2012-07-05 | 2015-04-22 | 贝克曼考尔特公司 | 用于确定白血细胞计数的方法和装置 |
| US20140186874A1 (en) * | 2012-12-31 | 2014-07-03 | Beckman Coulter, Inc. | Immature platelet enumeration systems and methods |
| CN104903699A (zh) * | 2012-12-31 | 2015-09-09 | 贝克曼考尔特公司 | 未成熟血小板的计数系统和方法 |
| CN103472216A (zh) * | 2013-08-23 | 2013-12-25 | 深圳中科强华科技有限公司 | 一种血细胞分析芯片、分析仪及分析方法 |
| CN103472034A (zh) * | 2013-08-23 | 2013-12-25 | 深圳中科强华科技有限公司 | 一种血细胞分析芯片、分析仪及分析方法 |
| CN106018771A (zh) * | 2015-03-27 | 2016-10-12 | 希森美康株式会社 | 血液分析装置及血液分析方法 |
| EP3258274A1 (en) * | 2016-06-17 | 2017-12-20 | Sysmex Corporation | Sample analyzer using a patient-specific detection history |
Non-Patent Citations (4)
| Title |
|---|
| KUNICKA, ET AL: "Improved Platelet Counting Using Two-Dimensional Laser Light Scatter", vol. 114, pages 283 - 289 * |
| 施紫微等: "MindrayBC-6800全自动血液细胞分析仪低值血小板临床性能评价", vol. 14, no. 14, pages 23 - 26 * |
| 李惠卿等: "血细胞分析仪计数血小板假性减少原因分析及对策探讨", vol. 13, no. 13, pages 764 - 767 * |
| 王凤等: "根据中国人群血小板生物参考区间修改及验证显微镜复检规则", 国际检验医学杂志, vol. 37, no. 09, 31 May 2016 (2016-05-31), pages 1190 - 1191 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114047109A (zh) * | 2022-01-11 | 2022-02-15 | 深圳市帝迈生物技术有限公司 | 一种样本分析仪及其计数方法 |
| CN114047109B (zh) * | 2022-01-11 | 2022-06-21 | 深圳市帝迈生物技术有限公司 | 一种样本分析仪及其计数方法 |
Also Published As
| Publication number | Publication date |
|---|---|
| US20210033593A1 (en) | 2021-02-04 |
| US20240133864A1 (en) | 2024-04-25 |
| WO2019206313A1 (zh) | 2019-10-31 |
| US11841358B2 (en) | 2023-12-12 |
| CN111801568B (zh) | 2024-05-24 |
| EP3789751B1 (en) | 2024-06-26 |
| EP3789751A1 (en) | 2021-03-10 |
| EP3789751A4 (en) | 2021-06-30 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US11105742B2 (en) | Nucleated red blood cell warning method and device, and flow cytometer using the same | |
| CN111801568B (zh) | 测定血小板浓度的方法及系统 | |
| CN111656161B (zh) | 样本分析仪异常的报警方法、系统及存储介质 | |
| US7208319B2 (en) | Method of measurement of nucleated red blood cells | |
| US7008792B2 (en) | Method of measurement of nucleated red blood cells | |
| US8148101B2 (en) | Method for classifying and counting bacteria in body fluids | |
| US6472215B1 (en) | Method of analyzing nucleated red blood cells in a blood sample | |
| CN111684264B (zh) | 血液分析方法、血液分析系统及存储介质 | |
| CN111684263A (zh) | 血液分析系统、血液分析仪、血液分析方法及存储介质 | |
| US9797824B2 (en) | Method for hematology analysis | |
| EP2694960A2 (en) | Identifying and enumerating early granulated cells (egcs) | |
| CN114450589A (zh) | 分析血液样本中红细胞方法及血液分析系统 | |
| US20240230621A9 (en) | Methods and systems for determining platelet concentration | |
| CN115201155A (zh) | 血液样本的检测方法和样本分析仪 | |
| CN115201156A (zh) | 分析血液样本中红细胞的方法及血液分析系统 | |
| CN115201269A (zh) | 应用电阻抗检测血小板的方法和血液分析系统 |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |