CN111789103A - 一种冷冻保存用解冻液及解冻方法 - Google Patents
一种冷冻保存用解冻液及解冻方法 Download PDFInfo
- Publication number
- CN111789103A CN111789103A CN201910282421.0A CN201910282421A CN111789103A CN 111789103 A CN111789103 A CN 111789103A CN 201910282421 A CN201910282421 A CN 201910282421A CN 111789103 A CN111789103 A CN 111789103A
- Authority
- CN
- China
- Prior art keywords
- thawing
- solution
- pva
- thawing solution
- ice
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000010257 thawing Methods 0.000 title claims abstract description 151
- 238000005138 cryopreservation Methods 0.000 title claims abstract description 24
- 238000000034 method Methods 0.000 title claims abstract description 18
- 239000000463 material Substances 0.000 claims abstract description 32
- 239000011664 nicotinic acid Substances 0.000 claims abstract description 25
- 230000003993 interaction Effects 0.000 claims abstract description 18
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 16
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 13
- 125000000524 functional group Chemical group 0.000 claims abstract description 6
- 239000000243 solution Substances 0.000 claims description 130
- 239000004372 Polyvinyl alcohol Substances 0.000 claims description 71
- 229920002451 polyvinyl alcohol Polymers 0.000 claims description 71
- 239000007853 buffer solution Substances 0.000 claims description 31
- OZFAFGSSMRRTDW-UHFFFAOYSA-N (2,4-dichlorophenyl) benzenesulfonate Chemical compound ClC1=CC(Cl)=CC=C1OS(=O)(=O)C1=CC=CC=C1 OZFAFGSSMRRTDW-UHFFFAOYSA-N 0.000 claims description 22
- 239000012591 Dulbecco’s Phosphate Buffered Saline Substances 0.000 claims description 22
- 229940024606 amino acid Drugs 0.000 claims description 21
- 235000001014 amino acid Nutrition 0.000 claims description 21
- 150000001413 amino acids Chemical class 0.000 claims description 21
- 239000005720 sucrose Substances 0.000 claims description 21
- 229920001184 polypeptide Polymers 0.000 claims description 18
- 102000004196 processed proteins & peptides Human genes 0.000 claims description 18
- 108090000765 processed proteins & peptides Proteins 0.000 claims description 18
- 229930006000 Sucrose Natural products 0.000 claims description 17
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims description 17
- 239000002253 acid Substances 0.000 claims description 15
- ONIBWKKTOPOVIA-BYPYZUCNSA-N L-Proline Chemical compound OC(=O)[C@@H]1CCCN1 ONIBWKKTOPOVIA-BYPYZUCNSA-N 0.000 claims description 12
- AYFVYJQAPQTCCC-GBXIJSLDSA-N L-threonine Chemical compound C[C@@H](O)[C@H](N)C(O)=O AYFVYJQAPQTCCC-GBXIJSLDSA-N 0.000 claims description 11
- AYFVYJQAPQTCCC-UHFFFAOYSA-N Threonine Natural products CC(O)C(N)C(O)=O AYFVYJQAPQTCCC-UHFFFAOYSA-N 0.000 claims description 9
- 239000004473 Threonine Substances 0.000 claims description 9
- 239000004475 Arginine Substances 0.000 claims description 8
- ODKSFYDXXFIFQN-UHFFFAOYSA-N arginine Natural products OC(=O)C(N)CCCNC(N)=N ODKSFYDXXFIFQN-UHFFFAOYSA-N 0.000 claims description 8
- 229960003121 arginine Drugs 0.000 claims description 8
- 239000000203 mixture Substances 0.000 claims description 7
- KDXKERNSBIXSRK-YFKPBYRVSA-N L-lysine Chemical compound NCCCC[C@H](N)C(O)=O KDXKERNSBIXSRK-YFKPBYRVSA-N 0.000 claims description 6
- RWRDLPDLKQPQOW-UHFFFAOYSA-N Pyrrolidine Chemical compound C1CCNC1 RWRDLPDLKQPQOW-UHFFFAOYSA-N 0.000 claims description 6
- PHOQVHQSTUBQQK-SQOUGZDYSA-N D-glucono-1,5-lactone Chemical compound OC[C@H]1OC(=O)[C@H](O)[C@@H](O)[C@@H]1O PHOQVHQSTUBQQK-SQOUGZDYSA-N 0.000 claims description 4
- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 claims description 4
- ODKSFYDXXFIFQN-BYPYZUCNSA-P L-argininium(2+) Chemical compound NC(=[NH2+])NCCC[C@H]([NH3+])C(O)=O ODKSFYDXXFIFQN-BYPYZUCNSA-P 0.000 claims description 4
- CKLJMWTZIZZHCS-REOHCLBHSA-N L-aspartic acid Chemical compound OC(=O)[C@@H](N)CC(O)=O CKLJMWTZIZZHCS-REOHCLBHSA-N 0.000 claims description 4
- ZDXPYRJPNDTMRX-VKHMYHEASA-N L-glutamine Chemical compound OC(=O)[C@@H](N)CCC(N)=O ZDXPYRJPNDTMRX-VKHMYHEASA-N 0.000 claims description 4
- 239000004472 Lysine Substances 0.000 claims description 4
- KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 claims description 4
- GVUVRRPYYDHHGK-VQVTYTSYSA-N Pro-Thr Chemical compound C[C@@H](O)[C@@H](C(O)=O)NC(=O)[C@@H]1CCCN1 GVUVRRPYYDHHGK-VQVTYTSYSA-N 0.000 claims description 4
- ONIBWKKTOPOVIA-UHFFFAOYSA-N Proline Natural products OC(=O)C1CCCN1 ONIBWKKTOPOVIA-UHFFFAOYSA-N 0.000 claims description 4
- HYLXOQURIOCKIH-VQVTYTSYSA-N Thr-Arg Chemical compound C[C@@H](O)[C@H](N)C(=O)N[C@H](C(O)=O)CCCNC(N)=N HYLXOQURIOCKIH-VQVTYTSYSA-N 0.000 claims description 4
- QOLYAJSZHIJCTO-VQVTYTSYSA-N Thr-Pro Chemical compound C[C@@H](O)[C@H](N)C(=O)N1CCC[C@H]1C(O)=O QOLYAJSZHIJCTO-VQVTYTSYSA-N 0.000 claims description 4
- 235000003704 aspartic acid Nutrition 0.000 claims description 4
- OQFSQFPPLPISGP-UHFFFAOYSA-N beta-carboxyaspartic acid Natural products OC(=O)C(N)C(C(O)=O)C(O)=O OQFSQFPPLPISGP-UHFFFAOYSA-N 0.000 claims description 4
- 239000000872 buffer Substances 0.000 claims description 4
- 239000003795 chemical substances by application Substances 0.000 claims description 4
- 230000009881 electrostatic interaction Effects 0.000 claims description 4
- 239000012634 fragment Substances 0.000 claims description 4
- 235000012209 glucono delta-lactone Nutrition 0.000 claims description 4
- 229910052739 hydrogen Inorganic materials 0.000 claims description 4
- 239000001257 hydrogen Substances 0.000 claims description 4
- 230000007062 hydrolysis Effects 0.000 claims description 4
- 238000006460 hydrolysis reaction Methods 0.000 claims description 4
- 230000002209 hydrophobic effect Effects 0.000 claims description 4
- 125000002887 hydroxy group Chemical group [H]O* 0.000 claims description 4
- 125000001997 phenyl group Chemical group [H]C1=C([H])C([H])=C(*)C([H])=C1[H] 0.000 claims description 4
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 claims description 4
- 230000003592 biomimetic effect Effects 0.000 claims description 3
- HDTRYLNUVZCQOY-UHFFFAOYSA-N α-D-glucopyranosyl-α-D-glucopyranoside Natural products OC1C(O)C(O)C(CO)OC1OC1C(O)C(O)C(O)C(CO)O1 HDTRYLNUVZCQOY-UHFFFAOYSA-N 0.000 claims description 2
- JKMHFZQWWAIEOD-UHFFFAOYSA-N 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid Chemical compound OCC[NH+]1CCN(CCS([O-])(=O)=O)CC1 JKMHFZQWWAIEOD-UHFFFAOYSA-N 0.000 claims description 2
- WHUUTDBJXJRKMK-UHFFFAOYSA-N Glutamic acid Natural products OC(=O)C(N)CCC(O)=O WHUUTDBJXJRKMK-UHFFFAOYSA-N 0.000 claims description 2
- 239000004471 Glycine Substances 0.000 claims description 2
- -1 L-Arg-L-Thr (RT) Chemical compound 0.000 claims description 2
- ODKSFYDXXFIFQN-BYPYZUCNSA-N L-arginine Chemical compound OC(=O)[C@@H](N)CCCN=C(N)N ODKSFYDXXFIFQN-BYPYZUCNSA-N 0.000 claims description 2
- DCXYFEDJOCDNAF-REOHCLBHSA-N L-asparagine Chemical compound OC(=O)[C@@H](N)CC(N)=O DCXYFEDJOCDNAF-REOHCLBHSA-N 0.000 claims description 2
- WHUUTDBJXJRKMK-VKHMYHEASA-N L-glutamic acid Chemical compound OC(=O)[C@@H](N)CCC(O)=O WHUUTDBJXJRKMK-VKHMYHEASA-N 0.000 claims description 2
- HNDVDQJCIGZPNO-YFKPBYRVSA-N L-histidine Chemical compound OC(=O)[C@@H](N)CC1=CN=CN1 HNDVDQJCIGZPNO-YFKPBYRVSA-N 0.000 claims description 2
- HDTRYLNUVZCQOY-WSWWMNSNSA-N Trehalose Natural products O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-WSWWMNSNSA-N 0.000 claims description 2
- HDTRYLNUVZCQOY-LIZSDCNHSA-N alpha,alpha-trehalose Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-LIZSDCNHSA-N 0.000 claims description 2
- 125000003368 amide group Chemical group 0.000 claims description 2
- 150000001720 carbohydrates Chemical class 0.000 claims description 2
- 125000003178 carboxy group Chemical group [H]OC(*)=O 0.000 claims description 2
- 125000002843 carboxylic acid group Chemical group 0.000 claims description 2
- 150000001875 compounds Chemical class 0.000 claims description 2
- 229960003681 gluconolactone Drugs 0.000 claims description 2
- 235000013922 glutamic acid Nutrition 0.000 claims description 2
- 239000004220 glutamic acid Substances 0.000 claims description 2
- ZDXPYRJPNDTMRX-UHFFFAOYSA-N glutamine Natural products OC(=O)C(N)CCC(N)=O ZDXPYRJPNDTMRX-UHFFFAOYSA-N 0.000 claims description 2
- 150000004676 glycans Chemical class 0.000 claims description 2
- HNDVDQJCIGZPNO-UHFFFAOYSA-N histidine Natural products OC(=O)C(N)CC1=CN=CN1 HNDVDQJCIGZPNO-UHFFFAOYSA-N 0.000 claims description 2
- 229940124280 l-arginine Drugs 0.000 claims description 2
- 150000002840 non-reducing disaccharides Chemical class 0.000 claims description 2
- 229920001282 polysaccharide Polymers 0.000 claims description 2
- 239000005017 polysaccharide Substances 0.000 claims description 2
- 125000000719 pyrrolidinyl group Chemical group 0.000 claims description 2
- 230000002528 anti-freeze Effects 0.000 claims 2
- 239000007788 liquid Substances 0.000 abstract description 25
- 210000002966 serum Anatomy 0.000 abstract description 10
- 239000013078 crystal Substances 0.000 abstract description 4
- 239000003344 environmental pollutant Substances 0.000 abstract description 4
- 230000003071 parasitic effect Effects 0.000 abstract description 4
- 231100000719 pollutant Toxicity 0.000 abstract description 4
- 230000008859 change Effects 0.000 abstract description 3
- 238000011084 recovery Methods 0.000 abstract description 3
- 241001465754 Metazoa Species 0.000 abstract description 2
- 230000001988 toxicity Effects 0.000 abstract description 2
- 231100000419 toxicity Toxicity 0.000 abstract description 2
- 210000000287 oocyte Anatomy 0.000 description 21
- LYCAIKOWRPUZTN-UHFFFAOYSA-N Ethylene glycol Chemical compound OCCO LYCAIKOWRPUZTN-UHFFFAOYSA-N 0.000 description 16
- 210000001161 mammalian embryo Anatomy 0.000 description 13
- 210000004027 cell Anatomy 0.000 description 12
- 230000000052 comparative effect Effects 0.000 description 12
- 230000004083 survival effect Effects 0.000 description 11
- 238000007710 freezing Methods 0.000 description 10
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 8
- 229960002429 proline Drugs 0.000 description 8
- 210000002257 embryonic structure Anatomy 0.000 description 7
- 230000008014 freezing Effects 0.000 description 7
- 239000000126 substance Substances 0.000 description 7
- 238000002156 mixing Methods 0.000 description 5
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 4
- 238000003760 magnetic stirring Methods 0.000 description 4
- 229910052757 nitrogen Inorganic materials 0.000 description 4
- 230000035558 fertility Effects 0.000 description 3
- 239000012530 fluid Substances 0.000 description 3
- 210000000056 organ Anatomy 0.000 description 3
- 230000003204 osmotic effect Effects 0.000 description 3
- 238000004321 preservation Methods 0.000 description 3
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 2
- 229910002092 carbon dioxide Inorganic materials 0.000 description 2
- 239000001569 carbon dioxide Substances 0.000 description 2
- 238000012258 culturing Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 239000012091 fetal bovine serum Substances 0.000 description 2
- 239000001963 growth medium Substances 0.000 description 2
- 238000010438 heat treatment Methods 0.000 description 2
- WGCNASOHLSPBMP-UHFFFAOYSA-N hydroxyacetaldehyde Natural products OCC=O WGCNASOHLSPBMP-UHFFFAOYSA-N 0.000 description 2
- 238000006116 polymerization reaction Methods 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 230000035939 shock Effects 0.000 description 2
- 210000001519 tissue Anatomy 0.000 description 2
- 150000008064 anhydrides Chemical class 0.000 description 1
- 239000012620 biological material Substances 0.000 description 1
- 210000000625 blastula Anatomy 0.000 description 1
- 230000032823 cell division Effects 0.000 description 1
- 210000000170 cell membrane Anatomy 0.000 description 1
- 230000019522 cellular metabolic process Effects 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 239000002577 cryoprotective agent Substances 0.000 description 1
- 230000006866 deterioration Effects 0.000 description 1
- 239000003085 diluting agent Substances 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 235000013601 eggs Nutrition 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000009472 formulation Methods 0.000 description 1
- 210000004602 germ cell Anatomy 0.000 description 1
- 230000002710 gonadal effect Effects 0.000 description 1
- 210000005260 human cell Anatomy 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 229920000724 poly(L-arginine) polymer Polymers 0.000 description 1
- 229920001308 poly(aminoacid) Polymers 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 238000001953 recrystallisation Methods 0.000 description 1
- 230000001172 regenerating effect Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
- 238000004062 sedimentation Methods 0.000 description 1
- 238000002054 transplantation Methods 0.000 description 1
- 238000004017 vitrification Methods 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/02—Preservation of living parts
- A01N1/0205—Chemical aspects
- A01N1/021—Preservation or perfusion media, liquids, solids or gases used in the preservation of cells, tissue, organs or bodily fluids
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/02—Preservation of living parts
- A01N1/0278—Physical preservation processes
- A01N1/0284—Temperature processes, i.e. using a designated change in temperature over time
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Dentistry (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Environmental Sciences (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
Description
物质 | 含量 |
PVA(mg mL<sup>-1</sup>) | 20 |
蔗糖(mol L<sup>-1</sup>) | 1.0 |
DPBS(V) | 余量 |
物质 | 含量 |
PVA(mg mL<sup>-1</sup>) | 20 |
蔗糖(mol L<sup>-1</sup>) | 0.5 |
DPBS(V) | 余量 |
物质 | 含量 |
PVA(mg mL<sup>-1</sup>) | 20 |
蔗糖(mol L<sup>-1</sup>) | 0.25 |
DPBS(V) | 余量 |
物质 | 含量 |
PVA(mg mL<sup>-1</sup>) | 20 |
DPBS(V) | 余量 |
物质 | 含量 |
聚-L-脯氨酸/(mg mL<sup>-1</sup>) | 10 |
PVA(mg mL<sup>-1</sup>) | 20 |
蔗糖(mol L<sup>-1</sup>) | 1.0 |
DPBS(V) | 余量 |
物质 | 含量 |
聚-L-脯氨酸/(mg mL<sup>-1</sup>) | 5.0 |
PVA(mg mL<sup>-1</sup>) | 20 |
蔗糖(mol L<sup>-1</sup>) | 0.5 |
DPBS(V) | 余量 |
物质 | 含量 |
PVA(mg mL<sup>-1</sup>) | 20 |
DPBS(V) | 余量 |
编号 | 平衡液 | 冷冻液 | 解冻液 | 冻卵总数 | 2小时后存活率% |
应用实例1 | a | A | 实施例1 | 53 | 96.5 |
应用实例2 | b | B | 实施例2 | 60 | 98.6 |
应用实例3 | c | C | 实施例1 | 44 | 94.7 |
对比实例1 | a | A | 对比例1 | 50 | 93.4 |
对比实例2 | b | B | 对比例1 | 39 | 89.7 |
对比实例3 | c | C | 对比例1 | 96 | 81.9 |
编号 | 平衡液 | 冷冻液 | 解冻液 | 胚胎总数 | 2小时后存活率% |
对比实例4 | c | C | 对比例1 | 39 | 82.0 |
应用实例4 | b | B | 实施例2 | 39 | 97.4 |
应用实例5 | a | A | 实施例1 | 37 | 97.1 |
Claims (10)
Priority Applications (8)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201910282421.0A CN111789103B (zh) | 2019-04-09 | 2019-04-09 | 一种冷冻保存用解冻液及解冻方法 |
SG11202110901QA SG11202110901QA (en) | 2019-04-09 | 2020-03-02 | Thawing fluid, preparation method therefor and use thereof |
KR1020217033906A KR20210142698A (ko) | 2019-04-09 | 2020-03-02 | 해동액 및 그 제조 방법과 응용 |
US17/594,345 US20220192180A1 (en) | 2019-04-09 | 2020-03-02 | Thawing fluid, preparation method therefor and use thereof |
EP20787891.9A EP3939429A4 (en) | 2019-04-09 | 2020-03-02 | DEFASTING FLUID, PROCESS FOR ITS PRODUCTION AND ITS USE |
PCT/CN2020/077475 WO2020207153A1 (zh) | 2019-04-09 | 2020-03-02 | 一种解冻液及其制备方法与应用 |
AU2020256873A AU2020256873B2 (en) | 2019-04-09 | 2020-03-02 | Thawing fluid, preparation method therefor and use thereof |
JP2021560654A JP7459130B2 (ja) | 2019-04-09 | 2020-03-02 | 融解液及びその調製方法並びに応用 |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201910282421.0A CN111789103B (zh) | 2019-04-09 | 2019-04-09 | 一种冷冻保存用解冻液及解冻方法 |
Publications (2)
Publication Number | Publication Date |
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CN111789103A true CN111789103A (zh) | 2020-10-20 |
CN111789103B CN111789103B (zh) | 2022-05-27 |
Family
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Family Applications (1)
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CN201910282421.0A Active CN111789103B (zh) | 2019-04-09 | 2019-04-09 | 一种冷冻保存用解冻液及解冻方法 |
Country Status (1)
Country | Link |
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CN (1) | CN111789103B (zh) |
Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2009247276A (ja) * | 2008-04-04 | 2009-10-29 | Erii Kk | 生体供給用、生体移植用、生体添加用、生体投与用の解凍器官若しくは組織または解凍細胞群とその製造方法及びそれらに用いる過冷却液並びに製造装置 |
CN103918642A (zh) * | 2014-04-17 | 2014-07-16 | 中国农业科学院北京畜牧兽医研究所 | 一种猪精液冷冻方法及其专用冷冻液 |
CN108244102A (zh) * | 2018-04-17 | 2018-07-06 | 北京大学第三医院 | 一种生殖冷冻用玻璃化冷冻试剂、试剂盒及其使用方法 |
CN108753698A (zh) * | 2018-06-05 | 2018-11-06 | 瑞柏生物(中国)股份有限公司 | 一种玻璃化解冻液及其制备方法 |
-
2019
- 2019-04-09 CN CN201910282421.0A patent/CN111789103B/zh active Active
Patent Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2009247276A (ja) * | 2008-04-04 | 2009-10-29 | Erii Kk | 生体供給用、生体移植用、生体添加用、生体投与用の解凍器官若しくは組織または解凍細胞群とその製造方法及びそれらに用いる過冷却液並びに製造装置 |
CN103918642A (zh) * | 2014-04-17 | 2014-07-16 | 中国农业科学院北京畜牧兽医研究所 | 一种猪精液冷冻方法及其专用冷冻液 |
CN108244102A (zh) * | 2018-04-17 | 2018-07-06 | 北京大学第三医院 | 一种生殖冷冻用玻璃化冷冻试剂、试剂盒及其使用方法 |
CN108753698A (zh) * | 2018-06-05 | 2018-11-06 | 瑞柏生物(中国)股份有限公司 | 一种玻璃化解冻液及其制备方法 |
Also Published As
Publication number | Publication date |
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CN111789103B (zh) | 2022-05-27 |
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