CN111743913A

CN111743913A - Baby shower gel, antibacterial agent for babies and preparation method thereof

Info

Publication number: CN111743913A
Application number: CN202010678102.4A
Authority: CN
Inventors: 苏格格
Original assignee: Individual
Current assignee: Individual
Priority date: 2020-07-15
Filing date: 2020-07-15
Publication date: 2020-10-09

Abstract

The invention provides baby shower gel, an antibacterial agent for babies and a preparation method thereof. The preparation method of the antibacterial agent for the infants comprises the following steps: s1, processing chitin; s2, preparing magnetic chitin hydrogel; s3, interlayer containing TiO₂The magnetic chitin hydrogel is prepared. The antibacterial agent is a plant-derived bacteriostatic agent, can be externally used for treating infectious skin diseases caused by various fungi or bacteria and the like, relieving wound infection and reducing skin pain or itching. The baby bath lotion prepared by the invention has no toxicity, no irritation, safety and effectiveness to skin, and has good bacteriostatic and antibacterial effects.

Description

Baby shower gel, antibacterial agent for babies and preparation method thereof

Technical Field

The invention relates to the technical field of antibacterial agents, and particularly relates to baby shower gel, an antibacterial agent for a baby and a preparation method of the antibacterial agent.

Background

Along with the improvement of the living standard of people, the consciousness of people to health is continuously strengthened. However, various pathogenic microorganisms are widely distributed in nature, and their growth and reproduction seriously threaten human health, especially infants, and the resistance is not high, and the environmental requirements are more strict. Thus, there is an increasing interest in the research and development of safe, highly effective and low cost antimicrobial agents.

Most of the existing antibacterial agents are release type, are usually loaded in a carrier, and enter the bacterial body through continuous release to further exert the antibacterial action. This leads to the decrease in antibacterial performance until the disappearance, and therefore, it is important to develop a non-releasing type antibacterial agent. The non-releasing antibacterial agent does not need to release the drug, but only needs to directly contact with the bacteria to achieve the purpose of killing the bacteria. The service time is prolonged as much as possible while the antibacterial requirement is met; in addition, the problem of drug toxicity residue can be avoided. Therefore, the modified antibacterial agent has lasting and stable antibacterial performance, low toxicity or no toxicity, and obviously improves the safety and health of human beings.

Disclosure of Invention

The invention aims to provide an antibacterial agent for infants and a preparation method thereof, which can be externally used for treating infectious skin diseases caused by various fungi or bacteria and the like, relieving wound infection and reducing skin pain or itching.

The technical scheme of the invention is realized as follows:

the invention provides a preparation method of an antibacterial agent for infants, which comprises the following steps:

s1, processing chitin: adding chitin powder into 5-10 wt% hydrochloric acid, stirring for reaction for 10-20 hr, and washing with water to neutrality; adding the mixture into 2-4 wt% NaOH solution, stirring for reaction for 10-20h, and washing with water to neutrality; adding into 20-45 wt% ethanol solution, stirring for 20-40h, washing with water to neutrality, and oven drying to constant weight to obtain pretreated chitin powder;

s2, preparing the magnetic chitin hydrogel: dissolving pretreated chitin powder in a solvent, adding cobalt chloride powder, stirring to dissolve to form hydrogel, adding 5-10 wt% NaOH solution, reacting for 0.5-2h, and washing with water to neutrality to obtain magnetic chitin hydrogel;

s3, interlayer containing TiO₂The preparation of the magnetic chitin hydrogel comprises the following steps: mixing magnetic chitin hydrogel and tetrabutyl titanate, and performing ultrasonic dispersion under a sealed condition until the magnetic chitin hydrogel and tetrabutyl titanate fully react; drying to obtain the antibacterial agent for infants.

s1, processing chitin: adding chitin powder into 5-10 wt% hydrochloric acid, stirring for reaction for 10-20 hr to remove calcium, filtering with deionized water, and repeatedly washing until the filtrate is neutral; adding the mixture into 2-4 wt% NaOH solution, stirring for reaction for 10-20h to remove residual protein, and washing to neutrality; adding into 20-45 wt% ethanol solution, stirring for 20-40h to remove color, washing with deionized water to neutrality, and oven drying to constant weight to obtain pretreated chitin powder;

s2, preparing the magnetic chitin hydrogel: dissolving pretreated chitin powder in a solvent, continuously stirring and adding cobalt chloride powder, stirring and dissolving, storing for a period of time at 30-40 ℃ to form hydrogel, adding the hydrogel into 5-10 wt% NaOH solution, continuously stirring and reacting for 0.5-2h, filtering, and washing the gel with water until the pH value is neutral to obtain magnetic chitin hydrogel;

s3, interlayer containing TiO₂The preparation of the magnetic chitin hydrogel comprises the following steps: mixing magnetic chitin hydrogel and tetrabutyl titanate, carrying out ultrasonic dispersion under a sealed condition until the mixture is fully reacted, and cleaning with absolute alcohol and carrying out suction filtration once; drying to obtain the interlayer containing TiO₂The magnetic chitin hydrogel, and the finally prepared interlayer TiO-containing₂The magnetic chitin hydrogel is an antibacterial agent for infants.

As a further improvement of the invention, the drying condition is 100-105 ℃ drying for 1-3 h.

As a further improvement of the invention, the solvent in step S2 is an aqueous solution containing 2-5 wt% of NaOH, 0.5-1 wt% of melamine and 10-20 wt% of ethanol.

As a further improvement of the invention, the mass ratio of the pretreated chitin powder to the cobalt chloride powder is 10: (3-5).

As a further improvement of the invention, the mass ratio of the magnetic chitin hydrogel to tetrabutyl titanate is 10: (1-3).

As a further improvement of the invention, the ultrasonic power is 1000-2000W.

The invention further protects the antibacterial agent for the infant prepared by the preparation method.

As a further improvement of the invention, the ice cream also comprises borneol.

As a further development of the invention, the interlayer contains TiO₂The mass ratio of the magnetic chitin hydrogel to the borneol is 10: (0.5-2).

As a further improvement of the invention, the dosage form is ointment or lotion.

The invention also provides baby bath cream which comprises the antibacterial agent for babies.

As a further improvement of the invention, the baby bath lotion comprises a surfactant and the antibacterial agent for babies, wherein the content of the antibacterial agent for babies is 0.05-1 wt%. The surfactant may be anionic surfactant, nonionic surfactant. The content of the surfactant is 5-20 wt%. The baby bath cream may also contain additives commonly used in the art, such as perfumes, pigments, thickeners, stabilizers, and the like.

Preferably, the baby bath lotion comprises the following components in percentage by weight: 3-6% of sodium cocoamidopropionate; 1-4% of sodium cocoyl methyl taurate; 3-6% of cocoyl amphodiacetate disodium; 0.05-0.2% of antibacterial agent for infants; the balance being water.

The invention has the following beneficial effects: the antibacterial agent interlayer prepared by the invention contains TiO₂Magnetic carapace ofTiO of plain hydrogel under photocatalysis₂The antibacterial agent can perform broad-spectrum antibacterial and has stable chemical properties, when no ultraviolet radiation exists, chitin contained in the antibacterial agent performs high-efficiency antibacterial, so that microbial protein is denatured, energy synthesis is blocked, cell metabolism is interfered, and a safe antibacterial effect is achieved;

chitin is a natural plant source antibacterial agent, the solubility of the chitin can be fully improved by dissolving the chitin in a solvent containing melamine to form hydrogel, cobalt chloride is added and fully stirred to be dissolved, inorganic particle points containing cobalt ions are formed in the chitin hydrogel, NaOH is further added to generate magnetic particle cobalt oxide, and thus magnetic hydrogel is prepared, the magnetic hydrogel and tetrabutyl titanate are subjected to hydrolysis reaction under appropriate water content to generate TiO₂，TiO₂Is a photocatalytic antibacterial agent, has broad-spectrum antibacterial effect, and further improves the antibacterial property of the material.

The antibacterial agent provided by the invention has obvious bacteriostatic and bactericidal effects, can inhibit and kill staphylococcus aureus, candida albicans and escherichia coli, has broad antibacterial spectrum and wide applicability, and can overcome the antibacterial drug resistance of antibiotics.

The antibacterial agent disclosed by the invention is prepared by mixing two components, can be used for treating infectious skin diseases caused by various fungi or bacteria and the like by external application, relieving wound infection and reducing skin pain or itching, is a plant-derived antibacterial agent, is natural, free of toxicity and irritation to skin, safe and effective, and has a good antibacterial effect and a small dosage. The baby bath lotion prepared by the invention has no toxicity, no irritation, safety and effectiveness to skin, and has good bacteriostatic and antibacterial effects.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

Chitin, CAS number 1398-64-1, available from Zhejiang gold shell biochemistry, Inc.;

cobalt chloride, CAS number 7646-79-9, purchased from the national pharmaceutical group;

melamine, CAS number 108-78-1, available from the national pharmaceutical group;

tetrabutyl titanate, CAS No. 3087-36-3, available from the national institutes of medicine.

Borneol, natural borneol sold by Hecheng Natural spice oil of Jiangxi Co., Ltd.

Example 1

S1, processing chitin: adding 100g of chitin powder into 100mL of 5 wt% hydrochloric acid, stirring for reaction for 10h to remove calcium, filtering with deionized water, and repeatedly washing until the filtrate is neutral; then adding the mixed solution into 100mL of 2 wt% NaOH solution, stirring and reacting for 10h to remove residual protein, and washing to be neutral; adding the chitin into 100mL of 20 wt% ethanol solution, stirring for reaction for 20h to remove color, washing with deionized water to be neutral, and drying to constant weight to obtain pretreated chitin powder;

s2, preparing the magnetic chitin hydrogel: dissolving 100g of pretreated chitin powder in 500mL of solvent (the solvent is an aqueous solution containing 2 wt% of NaOH, 0.5 wt% of melamine and 10 wt% of ethanol), continuously stirring and adding 30g of cobalt chloride powder, stirring and dissolving, storing for a period of time at 30 ℃ to form hydrogel, adding 200mL of 5 wt% of NaOH solution, continuously stirring and reacting for 0.5h, filtering, washing the gel with water until the pH value is neutral, and obtaining the magnetic chitin hydrogel;

s3, interlayer containing TiO₂The preparation of the magnetic chitin hydrogel comprises the following steps: mixing 100g of magnetic chitin hydrogel and 10g of tetrabutyl titanate, carrying out ultrasonic dispersion under a sealed condition until full reaction is achieved, and cleaning with absolute alcohol and carrying out suction filtration once; drying to obtain the interlayer containing TiO₂The magnetic chitin hydrogel, and the finally prepared interlayer TiO-containing₂The magnetic chitin hydrogel is the antibacterial agent for the infant in the embodiment.

In the embodiment, the drying condition is drying for 1h at 100 ℃, and the ultrasonic power is 1000W.

Example 2

S1, processing chitin: adding 100g of chitin powder into 100mL of 10 wt% hydrochloric acid, stirring for reaction for 20h to remove calcium, filtering with deionized water, and repeatedly washing until the filtrate is neutral; then adding the mixed solution into 100mL of 4 wt% NaOH solution, stirring and reacting for 20 hours to remove residual protein, and washing to be neutral; adding the chitin into 100mL of 45 wt% ethanol solution, stirring for reaction for 40h to remove color, washing with deionized water to be neutral, and drying to constant weight to obtain pretreated chitin powder;

s2, preparing the magnetic chitin hydrogel: dissolving 100g of pretreated chitin powder in 500mL of solvent (the solvent is aqueous solution containing 5 wt% of NaOH, 1 wt% of melamine and 20 wt% of ethanol), continuously stirring and adding 40g of cobalt chloride powder, stirring and dissolving, storing for a period of time at 40 ℃ to form hydrogel, adding 200mL of 10 wt% of NaOH solution, continuously stirring and reacting for 2 hours, filtering, washing the gel with water until the pH value is neutral, and obtaining the magnetic chitin hydrogel;

s3, interlayer containing TiO₂The preparation of the magnetic chitin hydrogel comprises the following steps: mixing 100g of magnetic chitin hydrogel and 30g of tetrabutyl titanate, carrying out ultrasonic dispersion under a sealed condition until full reaction is achieved, and cleaning with absolute alcohol and carrying out suction filtration once; drying to obtain the interlayer containing TiO₂The magnetic chitin hydrogel, and the finally prepared interlayer TiO-containing₂The magnetic chitin hydrogel is the antibacterial agent for the infant in the embodiment.

In the embodiment, the drying condition is drying at 105 ℃ for 3h, and the ultrasonic power is 2000W.

Example 3

S1, processing chitin: adding 100g of chitin powder into 100mL of 7 wt% hydrochloric acid, stirring for reaction for 15h to remove calcium, and washing with deionized water to be neutral; then adding the mixed solution into 100mL of 3 wt% NaOH solution, stirring and reacting for 15h to remove residual protein, and washing with deionized water to be neutral; adding 100mL of 32 wt% ethanol aqueous solution, stirring for reaction for 30h to remove color, washing with deionized water to neutrality, and drying to constant weight to obtain pretreated chitin powder;

s2, preparing the magnetic chitin hydrogel: dissolving 100g of pretreated chitin powder in 500mL of solvent (the solvent is an aqueous solution containing 3.5 wt% of NaOH, 0.7 wt% of melamine and 15 wt% of ethanol), continuously stirring and adding 40g of cobalt chloride powder, stirring and dissolving, storing at 35 ℃ for 20min to form hydrogel, adding 200mL of 7 wt% of NaOH solution, continuously stirring and reacting for 1.5h, filtering, washing the gel with water until the pH value is neutral, and obtaining the magnetic chitin hydrogel;

s3, interlayer containing TiO₂The preparation of the magnetic chitin hydrogel comprises the following steps: mixing 100g of the magnetic chitin hydrogel with 20g of tetrabutyl titanate, carrying out ultrasonic dispersion under a sealed condition until the magnetic chitin hydrogel and the tetrabutyl titanate fully react, and cleaning with absolute alcohol and carrying out suction filtration once; drying to constant weight to obtain interlayer TiO-containing material₂The magnetic chitin hydrogel, and the finally prepared interlayer TiO-containing₂The magnetic chitin hydrogel is the antibacterial agent for the infant in the embodiment.

In the embodiment, the drying condition is drying at 102 ℃ for 2h, and the ultrasonic power is 1500W.

Comparative example 1

Compared to example 3, no melamine was added, and the other conditions were unchanged.

s2, preparing the magnetic chitin hydrogel: dissolving 100g of pretreated chitin powder in 500mL of solvent (the solvent is aqueous solution containing 3.5 wt% of NaOH and 15 wt% of ethanol), continuously stirring and adding 40g of cobalt chloride powder, stirring and dissolving, storing at 35 ℃ for 20min to form hydrogel, adding 200mL of 7 wt% of NaOH solution, continuously stirring and reacting for 1.5h, filtering, and washing the gel with water until the pH value is neutral to obtain the magnetic chitin hydrogel;

Comparative example 2

Compared with example 3, no cobalt chloride was added, and other conditions were not changed.

s2, preparing the magnetic chitin hydrogel: dissolving 100g of pretreated chitin powder in 500mL of solvent (the solvent is an aqueous solution containing 3.5 wt% of NaOH, 0.7 wt% of melamine and 15 wt% of ethanol), storing at 35 ℃ for 20min to form hydrogel, adding into 200mL of 7 wt% NaOH solution, continuously stirring and reacting for 1.5h, filtering, and washing the gel with water until the pH value is neutral to obtain chitin hydrogel;

s3, interlayer containing TiO₂The preparation of the chitin hydrogel comprises the following steps: mixing 100g of the chitin hydrogel and 20g of tetrabutyl titanate, carrying out ultrasonic dispersion under a sealed condition until the chitin hydrogel and the tetrabutyl titanate fully react, and cleaning with absolute alcohol and carrying out suction filtration once; drying to constant weight to obtain interlayer TiO-containing material₂The magnetic chitin hydrogel, and the finally prepared interlayer TiO-containing₂The magnetic chitin hydrogel is the antibacterial agent for the infant in the embodiment.

Comparative example 3

In comparison with example 3, tetrabutyl titanate was not added, and other conditions were not changed.

s2, preparing the magnetic chitin hydrogel: dissolving 100g of pretreated chitin powder in 500mL of solvent (the solvent is an aqueous solution containing 3.5 wt% of NaOH, 0.7 wt% of melamine and 15 wt% of ethanol), continuously stirring and adding 40g of cobalt chloride powder, stirring and dissolving, storing at 35 ℃ for 20min to form hydrogel, adding 200mL of 7 wt% of NaOH solution, continuously stirring and reacting for 1.5h, filtering, washing the gel with water until the pH value is neutral, and obtaining the magnetic chitin hydrogel, namely the antibacterial agent for infants in the embodiment.

Example 4

The formula is as follows:

main drugs: interlayer TiO-containing layer from example 3₂10g of magnetic chitin hydrogel and 1.5g of borneol;

matrix: 90g of vaseline and 10g of lanolin;

the preparation process of the ointment preparation comprises the following steps:

(1) weighing the main drugs according to the formula ratio respectively, and grinding the borneol therein;

(2) weighing vaseline and lanolin with a weight of half of the formula, and grinding the vaseline and lanolin uniformly to obtain ointment matrix;

(3) the interlayer contains TiO₂The magnetic chitin hydrogel and the borneol are uniformly mixed and added in an equivalent and increasing modeAdding into ointment matrix, grinding to obtain compound Borneolum antibacterial ointment, sealing, and storing in shade or cold storage.

Example 5

The formula is as follows:

main drugs: interlayer TiO-containing coating prepared in comparative example 1₂10g of magnetic chitin hydrogel and 1.5g of borneol;

matrix: 90g of vaseline and 10g of lanolin;

(3) the interlayer contains TiO₂The magnetic chitin hydrogel and borneol are mixed uniformly, added into the ointment matrix in an equivalent and increasing mode, and ground uniformly to obtain the compound borneol antibacterial ointment, and the compound borneol antibacterial ointment is sealed and stored in a cool place or in a cold storage place.

Example 6

The formula is as follows:

main drugs: interlayer TiO-containing prepared in comparative example 2₂10g of chitin hydrogel and 1.5g of borneol;

matrix: 90g of vaseline and 10g of lanolin;

(3) the interlayer contains TiO₂Mixing the chitin hydrogel and Borneolum Syntheticum, adding into ointment matrix in the form of equivalent increment, grinding, and sealing, and storing in shade or cold storage.

Example 7

The formula is as follows:

main drugs: 10g of the magnetic chitin hydrogel prepared in the embodiment 3 and 1.5g of borneol;

matrix: 90g of vaseline and 10g of lanolin;

(3) mixing magnetic chitin hydrogel and Borneolum Syntheticum, adding into ointment matrix in the form of equivalent increment, grinding to obtain compound Borneolum antibacterial ointment, sealing, and storing in shade or cold storage.

Comparative example 4

Compared with example 4, borneol is not added in the main drug.

The formula is as follows:

main drugs: interlayer TiO-containing layer from example 3₂11.5g of the magnetic chitin hydrogel;

matrix: 90g of vaseline and 10g of lanolin;

(1) weighing the main medicines according to the formula ratio;

(3) the interlayer contains TiO₂The magnetic chitin hydrogel is added into ointment matrix in an equivalent increasing manner, and is uniformly ground to obtain the compound borneol antibacterial ointment, and the compound borneol antibacterial ointment is sealed and stored in a cool place or refrigerated storage.

Comparative example 5

Compared with the embodiment 4, the interlayer TiO-containing main drug is not added₂The magnetic chitin hydrogel.

The formula is as follows:

main drugs: 11.5g of borneol;

matrix: 90g of vaseline and 10g of lanolin;

(1) weighing the main medicines according to the formula ratio, and grinding the borneol therein;

(3) adding Borneolum into ointment matrix in the form of equivalent increment, grinding, and sealing, and storing in shade or cold storage.

Test example 1: in vitro antibacterial experiments

1 Experimental strains

Staphylococcus aureus (Staphylocoussureus, ATCC6538), Candida albicans (ATCC 10231), Escherichia coli (Migula) Castellaniet Churmer, ATCC 8099);

2 drugs and reagents

Sodium chloride (analytically pure AR), tryptone, yeast extract, agar powder, and PBS buffer solution

Culture medium: beef extract soup liquid culture medium: self-made beef extract soup liquid culture medium (bacterial culture medium) is prepared: weighing beef extract 2.0g, peptone 2.0g and sodium chloride 1.0g, placing into a beaker, adding a small amount of distilled water, heating to melt, adding distilled water to 200ml, adjusting pH to 7.4-7.6 with 20g/dl sodium hydroxide solution, filtering with filter paper, packaging with a triangular flask, and sterilizing at 103.4kPa for 20 minutes.

Liquid saxabeka medium (SD) (fungal medium): purchased from institute of microbiology, academy of sciences, Guangdong province, and autoclaved at 115 ℃ for 15 minutes for future use.

Equipment: test tubes, pipettes, test tube racks, small Erlenmeyer flasks, inoculating loops, 50 and 100 μ L sample applicators, clean benches, small electrothermal sterilizers, and biochemical incubators.

Reagent: 1mol/L hydrochloric acid; 200g/L of sodium hydroxide solution; methanol.

3 antimicrobial agent

Antibacterial agents obtained in examples 4 to 7 and comparative examples 4 and 5

Penicillin sodium 80 ten thousand units (BS);

ketoconazole (KCZ);

compound beclomethasone camphor cream (BZ);

under aseptic operation, the antibacterial agents and the reference substance are respectively dissolved by methanol and the volume is determined to prepare stock solution with the drug content of 10mg/ml, and the stock solution is sealed and stored in a refrigerator at 4 ℃ in dark for later use.

4 Experimental methods

(1) anti-Staphylococcus aureus test

Diluting the liquid medicine by a two-fold dilution method of the liquid medicine in each row of test tubes, namely a broth dilution method, adding 10mg/ml of stock solution of the antibacterial agent of example 4, 10mg/ml of stock solution of the antibacterial agent of comparative example 1, 10mg/ml of stock solution of the antibacterial agent of comparative example 2, 10mg/ml of stock solution of penicillin sodium, 10mg/ml of stock solution of ketoconazole and 10mg/ml of stock solution of compound beclomethasone camphor cream into a beef extract liquid culture medium to obtain a concentration of 1280 mu g/ml, adding 2ml of the diluted culture solution containing different antibacterial liquid medicines into each row of test tubes 1 and 2, mixing the test tubes 2, sucking out 2ml of liquid into a test tube 3, mixing the test tubes 3, sucking out the test tubes 4, sequentially diluting the test tubes 10 mu g to obtain a concentration of 2560 g/ml of the antibacterial liquid medicine, inoculating the antibacterial liquid to a concentration of staphylococcus aureus of 355 mu g/ml, inoculating the staphylococcus aureus suspension to a concentration of 355 g/ml of 2.5 g/ml of the bacteria suspension 2 g/ml, and inoculating the staphylococcus aureus to a concentration of each test tube 10.5 g/ml of 10 g/ml of the test tubes 2 g/ml of the test tubes 10.5 g/ml of the mixture of the test tubes 2 g/ml of the bacteria suspension 2 g/ml, and the test tubes 2 g/ml of the bacteria are added into the test tubes respectively⁴CFU/ml), adding 2ml of beef soup culture solution into each row of test tubes No. 12 as blank control, and placing all the test tubes in a constant temperature incubator at 37 ℃ for 18 h.

The highest dilution of the drug, which is observed by naked eyes without turbidity or precipitation and color change, namely the lowest drug concentration without visible bacteria growth by naked eyes is taken as the Minimum Inhibitory Concentration (MIC) of the drug to the strain.

0.05ml of the broth from the above test tube in which no bacteria were visible to the naked eye were streaked onto nutrient agar plates, incubated at 35 ℃ for 24 hours in an incubator, and the nutrient agar plates incubated at 35 ℃ for 24 hours were removed, and the lowest bactericidal concentration (end point of MBC) was determined as the lowest drug concentration (highest dilution) for growth of the colonies on the plates.

(2) anti-Candida albicans experiment

The same procedure as in example 1 was followed for Staphylococcus aureus resistance except that the added species Staphylococcus aureus was replaced with Candida albicans and 10mg/ml penicillin sodium stock solution was replaced with 10mg/ml ketoconazole stock solution, and the results are shown in Table 1 below.

(3) Experiment for resistance to Escherichia coli

Except that the added strain staphylococcus aureus is replaced by escherichia coli, 10mg/ml of penicillin sodium stock solution, penicillin sodium and compound beclomethasone camphor cream are removed for the experiment of the antibacterial performance, and the experiment is reduced to 5 rows of test tubes, the method is the same as the method for the experiment of the staphylococcus aureus resistance in example 1, and the result is shown in the following table 1.

TABLE 1 results of antibacterial assay

Strain name	Medicine	MIC(μg/mL)	MBC(μg/mL)
				Staphylococcus aureus	Example 4	40	80
Staphylococcus aureus	Example 5	160	320
				Staphylococcus aureus	Example 6	80	160
Staphylococcus aureus	Example 7	320	640
				Staphylococcus aureus	Comparative example 1	320	640
Staphylococcus aureus	Comparative example 2	640	1280
				Staphylococcus aureus	BS	80	320
Staphylococcus aureus	KCZ	320	640
				Staphylococcus aureus	BZ	160	320
Candida albicans	Example 4	20	40
				Candida albicans	Example 5	80	160
Candida albicans	Example 6	40	80
				Candida albicans	Example 7	160	320
Candida albicans	Comparative example 1	160	320
				Candida albicans	Comparative example 2	320	640
Candida albicans	BS	320	640
				Candida albicans	KCZ	40	80
Candida albicans	BZ	80	640
				Escherichia coli	Example 4	10	40
Escherichia coli	Example 5	40	160
				Escherichia coli	Example 6	20	80
Escherichia coli	Example 7	80	320
				Escherichia coli	Comparative example 1	160	320
Escherichia coli	Comparative example 2	80	160
				Escherichia coli	BS	320	640
Escherichia coli	KCZ	640	1280
				Escherichia coli	BZ	320	640

Note: penicillin sodium (BS), Ketoconazole (KCZ) and compound beclomethasone camphor cream (BZ).

As can be seen from table 1, the antibacterial agent provided in example 4 of the present invention has inhibitory and killing effects on 3 common pathogenic strains, i.e., staphylococcus aureus, candida albicans, and escherichia coli, and the bacteriostatic effect is similar to or better than that of antibiotics. Example 5 interlayer TiO-containing prepared using comparative example 1₂The magnetic chitin hydrogel in comparative example 1 has no melamine added, and the dissolubility of chitin is reduced, so that the interlayer contains TiO₂The chitin content in the magnetic chitin hydrogel is reduced, so that the antibacterial property of the magnetic chitin hydrogel is reduced; example 6 interlayer TiO-containing Using comparative example 2₂The antibacterial property of the chitin hydrogel is not obviously reduced, and the hydrogel does not contain magnetic particles, so that the antibacterial property is not greatly influenced, and the removal convenience of the hydrogel is mainly influenced to a certain extent; example 7 the magnetic chitin hydrogel prepared in comparative example 3, which did not contain photocatalytic bacteriostatic agent TiO, was used₂The antibacterial property of the chitin is obviously reduced, and the antibacterial effect of the chitin alone is only the antibacterial effect of the chitin alone.

The components provided by the invention are compatible, compared with borneol and TiO contained in the interlayer₂The magnetic chitin hydrogel and other single components have better bacteriostatic effects on staphylococcus aureus, candida albicans and escherichia coli, and show the superiority of the composite component in bacteriostatic effect and antibacterial broad spectrum.

Test example 2: in vitro toxicology test

(1) Skin irritation test

1 laboratory animal

White big-ear rabbits, all males, weighing 3.0 + -0.2 Kg, were provided by the pharmaceutical research institute of Jiangxi province. The feeding was observed in the laboratory for one week before the experiment. During the test, the animals were raised in cages with 1 cage per cage, and water was freely fed. The temperature of the animal feeding room is 20 +/-4 ℃, and the relative humidity is 50% +/-10%.

2 method of experiment

The irritant effect of the antimicrobial of example 4 on damaged skin and intact skin was observed using the in vivo self control.

Grouping: randomly taking 12 white rabbits with similar body weight, and dividing the rabbits into 2 groups, 6 rabbits in each group, and respectively taking the rabbits in each half of male and female groups as a complete skin group and a damaged skin group.

The experimental method comprises the following steps: in 1 day before the experiment, the white rabbits with big ears are prepared with skin, and the two sides of the spinal column at the back are unhaired, and the area of the unhaired rabbit occupies about 10% of the whole body. On the day of the experiment, the affected skin groups were disinfected with alcohol on the depilated area and 2 local anesthetized sites were randomly placed on the backs of the white big-ear rabbits with lidocaine hydrochloride solution. After 5 minutes, 2 crosshairs are scratched by a scalpel in the hair removing area, and skin lesions on the left side and the right side are consistent as much as possible by taking mild blood seepage as a standard. After the blood flow had stopped, the animals were each treated by applying 1ml of the antibacterial ointment (the antibacterial agent of example 4 above) to the depilated area on the left side and an equal amount of the base (the same as in the antibacterial agent of example 4 above, except that no main agent was added) to the right side. Wrapping and fixing with sterile gauze, and breeding in cages. The intact skin group was similarly used with the autologous control. After 24 hours, the drugs were washed away with warm water, and the presence of erythema edema at the administration site was observed at 1 hour, 12 hours, 24 hours, 48 hours, 72 hours, 4 days, and 5 days after the removal of the drugs. The evaluation was performed according to the skin irritation response scoring criteria in the guidelines for the study of new drugs in Chinese medicine (pharmacy, pharmacology, toxicology), and the mean values were calculated, the results are shown in table 2.

The observation results show that the left and right side areas of the intact skin and the damaged skin groups after being coated with the antibacterial agent provided by the invention have no obvious erythema and water phenomenon, and the coated parts have no pigmentation and bleeding points, and in addition, the antibacterial agent provided by the invention has no irritation to the skin as can be seen from the table 2.

(2) Skin allergy test

Grouping: the weight of the big-ear white rabbit is 3.0 +/-0.2 Kg, which is provided by the pharmaceutical research institute in Jiangxi province. The feeding was observed in the laboratory for one week before the experiment. During the test, the animals were raised in cages with 1 cage per cage, and water was freely fed. The temperature of the animal feeding room is 20 +/-4 ℃, and the relative humidity is 50% +/-10%.

Randomly taking 18 big-ear white rabbits with similar body weights, and equally dividing the rabbits into 3 groups, wherein each group comprises 6 rabbits, and the male rabbits and the female rabbits are respectively half-female rabbits: group a-compound borneol antimicrobial (example 4 antimicrobial above), group B-matrix (same as in example 4 antimicrobial above, except that no primary drug was added), group C-control (1% sensitization concentration 2, 4-dinitrochlorobenzene solution, DNCB).

② Experimental method, 1 day before administration, big ear white rabbit is preserved skin, and its back spine left side is unhaired with 3 × 3cm of each side². On the day of experiment, the compound borneol antibacterial agent, the matrix and 0.2ml of 1% DNCB are respectively smeared on the hair removal area on the left side of A, B, C groups of animals, bound and fixed by gauze, and raised in cages. After 6 hours of administration, the corresponding material was washed off. The experiment was repeated 1 time each on day 7 and 14 in the same manner for a total of 3 stimulations. 14 days after the last administration, the right side of each group of rabbits was dehaired and coated with the corresponding treatment in the same manner. Removing the test substance after 6 hr, immediately observing skin allergy, and observing skin allergy 1, 24, 48, and 72 hr after removing the medicine while observing whether the animal has severe systemic anaphylaxis such as asthma, unstable standing or shock.

The experimental results were evaluated according to the scoring criteria for the degree of skin allergy in the guidelines for research on new drugs in Chinese medicine (pharmacy, pharmacology, toxicology), and the results are shown in table 2.

TABLE 2 results of skin allergy test for antibacterial agent

The observation result shows that no erythema edema phenomenon appears on the skins of both sides of the white rabbits coated with the antibacterial agent provided by the invention, and the table 2 also shows that the antibacterial agent provided by the invention has no sensitization to the skins.

In conclusion, the antibacterial agent provided by the invention has good antibacterial and bacteriostatic effects and broad antibacterial spectrum, can be externally used, has no irritation and allergy to skin, and is safe and nontoxic.

Borneol (Borneol), also known as Borneol. Borneol plays an important unique and assistant role as a representative medicine of aromatic resuscitation inducing medicines. At present, many studies and reports on the antibacterial property of borneol at home and abroad are carried out, and in vitro antibacterial experiments on 14 common pathogenic strains show that borneol has obvious antibacterial effect on staphylococcus aureus, drug-resistant staphylococcus aureus and staphylococcus albus, has the antibacterial effect at a lower concentration and the antibacterial effect at a high concentration, and the antibacterial effect is enhanced along with the increase of action time and is weakened along with the reduction of drug concentration. (Unlum, Daferera D, DonmezE, Polissioum, TepeB, Sokmean. compositions and the in vitro antimicrobial activities of the infection oils of Achilleaceae and Achillea tertifolia [ J]JEthnopharmacol, 2002; 83: 117-21; study on in vitro antibacterial action of borneol and isoborneol, such as muji bowl, Yanghua, Sun Yumei, etc. [ J ]]20-22 in journal of Waxis pharmacy, 1989,4 (l); study on in vitro antibacterial activity of 3 dosage forms of Borneolum Syntheticum [ J)]The Proc of Jiangxi traditional Chinese medicine college, 2005,17(1):63-65.), simultaneously, borneol shows good effect against fungi, fungus strains such as aspergillus niger and the like are separated from secretion of external auditory canal of patients with suppurative otitis media, and observation by an electron microscope shows that under the action of borneol, the cell membrane and cytoplasm of aspergillus niger are damaged to generate a large number of electron blank areas (ETA), mitochondria deform, hypha shrink, distortion deformation, and fungus dissolution and death (Changsiping, Liyuchun and the like, the influence of borneol on the superfine structure of fungus cells and clinical application for treating suppurative otitis media [ J]InChina Chinese medicine journal, 2000,25(5): 306-. The natural borneol mainly comprises d-borneol and also comprises various sesquiterpenes including humulene, caryophyllene and the like. The natural borneol contains triterpenoid compounds such as oleanolic acid, hordenic acid and the like besides sesquiterpene, the chitin is also a natural plant source antibacterial agent, the natural borneol and the chitin are combined, the antibacterial mechanism is mainly dissolved and active, so that the microbial protein is denatured, the energy synthesis is blocked, the cell metabolism is interfered, and meanwhile, the natural borneol contains a photocatalytic antibacterial agent TiO₂And the antibacterial effect is further enhanced under the irradiation of ultraviolet light, so that the dosage of the antibacterial agent is effectively reduced, and the synergistic effect is realized.

Compared with the prior art, the antibacterial agent interlayer prepared by the invention contains TiO₂The magnetic chitin hydrogel is TiO under photocatalysis₂The antibacterial agent can perform broad-spectrum antibacterial and has stable chemical properties, when no ultraviolet radiation exists, chitin contained in the antibacterial agent performs high-efficiency antibacterial, so that microbial protein is denatured, energy synthesis is blocked, cell metabolism is interfered, and a safe antibacterial effect is achieved;

The antibacterial agent disclosed by the invention is prepared by mixing two components, can be used for treating infectious skin diseases caused by various fungi or bacteria and the like by external application, relieving wound infection and reducing skin pain or itching, is a plant-derived antibacterial agent, is natural, free of toxicity and irritation to skin, safe and effective, and has a good antibacterial effect and a small dosage.

Example 8

The baby bath lotion is produced by adopting a conventional method in the industry, and has the following specific formula: (percent by mass)

Sodium cocoamidopropionate 5%;

sodium cocoyl methyl taurate 3%;

5% of disodium cocoyl amphodiacetate;

0.1 wt% of the antibacterial agent for infants of example 7;

the balance being water.

The baby bath lotion prepared by the invention has no toxicity, no irritation, safety and effectiveness to skin, and has good bacteriostatic and antibacterial effects.

The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents, improvements and the like that fall within the spirit and principle of the present invention are intended to be included therein.

Claims

1. A method for preparing an antibacterial agent for infants, comprising the steps of:

2. A method for preparing an antibacterial agent for infants, comprising the steps of:

3. The method for preparing the antibacterial agent for infants according to claim 1 or 2, wherein the drying condition is 100-105 ℃ drying for 1-3 h.

4. The method of claim 1 or 2, wherein the solvent in step S2 is an aqueous solution containing 2-5 wt% NaOH, 0.5-1 wt% melamine, and 10-20 wt% ethanol.

5. The method for preparing an antibacterial agent for infants according to claim 1 or 2, wherein the mass ratio of the pretreated chitin powder to the cobalt chloride powder is 10: (3-5).

6. The method for preparing an antibacterial agent for infants according to claim 1 or 2, wherein the mass ratio of the magnetic chitin hydrogel to the tetrabutyl titanate is 10: (1-3); the ultrasonic power is 1000-2000W.

7. An antibacterial agent for infants prepared by the preparation method according to any one of claims 1 to 6.

8. The antibacterial agent for infants according to claim 7, further comprising borneol.

9. The infant antiseptic of claim 8 wherein said interlayer comprises TiO₂The mass ratio of the magnetic chitin hydrogel to the borneol is 10: (0.5-2).

10. A baby bath comprising a surfactant and the infant antibacterial agent of any one of claims 1 to 9, wherein the surfactant is present in an amount of 5 to 20 wt%, and the infant antibacterial agent is present in an amount of 0.05 to 1 wt%;