CN103356738A - Skin disinfection gel and its application - Google Patents
Skin disinfection gel and its application Download PDFInfo
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- CN103356738A CN103356738A CN2013103052272A CN201310305227A CN103356738A CN 103356738 A CN103356738 A CN 103356738A CN 2013103052272 A CN2013103052272 A CN 2013103052272A CN 201310305227 A CN201310305227 A CN 201310305227A CN 103356738 A CN103356738 A CN 103356738A
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Abstract
Belonging to the technical field of disinfection, the invention discloses a skin disinfection gel to solve the disadvantages of existing disinfection technologies, especially preoperative disinfection technologies. The skin disinfection gel is composed of the following active components by mass: 1.5%-6% of chitosan, 1.5-10% of chitosan oligosaccharide, 2%-8% of baicalin, 0.5%-2% of chlorhexidine, 0.5%-1.5% of sodium alginate, 2%-5% of agarose, and 1.5%-5% of a mint extract. And the disinfection effect is good. The disinfection gel disclosed in the invention can be used for hand disinfection or skin disinfection in sanitary disinfection or preoperative disinfection, and with excellent disinfection effects and lasting function, it has very good application prospects and economic benefits.
Description
Technical field
The invention belongs to the disinfectant technical field, be specifically related to a kind of skin degerming gel and application thereof.
Background technology
Antibacterial on the skin can be divided into stays bacterium and resident bacterium temporarily.Resident bacterium is lived in below the keratodermatitis epithelial cell, also can find at skin surface.Resident bacterium has: resists external microorganism and in microecosystem, competes two major protective functions of nutrition, and generally seldom relevant with infection.Stay temporarily bacterium, generally be attached to the skin epithelial layer, can skin surface survival and part breeding.Stay temporarily bacterium and often directly contact near the environmental surfaces that obtains or contact the patient by medical personnel and patient and pollute, itself and hospital infection are closely related.Therefore clinical various treatments, diagnosis all need skin degerming; Sterilization is the important step of infection control, and the success or not of sterilization directly affects therapeutic quality.Select the skin antiseptic of high-efficiency low-toxicity then to become whether successful key factor of sterilization.
Skin antiseptic is of a great variety in the market, and clinical common skin antiseptic mainly contains ethanol, iodine and povidone iodine, chlorhexidine, quaternary ammonium compound, triclosan etc.Wherein 1) ethanol has reliable bactericidal action, wherein the alcoholic solution bactericidal effect of 60. 0%-80.0% valid density is best, is clinical the most frequently used concentration, but because its fungicidal spectrum is narrower, killing effect is relatively poor, uses separately the alcoholic solution sterilization can not satisfy the needs of clinical disinfection.2) iodine and Iodic agent are by the skin antiseptic of wide clinical application.Clinical Iodic agent commonly used mainly is iodine tincture, iodine water and povidone iodine.Povidone iodine is the current topmost iodine skin antiseptic that contains, and is the indefinite form complex that iodine and surfactant are combined into.Its contained surface active ingredient can change solution to the wettability of object, can form one deck sterilization thin film as thin as a wafer at skin surface.Have the iodine of assistance and penetrate the Organic substance effect, and the energy chyle fat, slowly lasting release available iodine is strengthened the bactericidal action of iodine.
Chlorhexidine belongs to the biguanides antibacterial, has the characteristics such as toxicity is low, zest is little, has a broad antifungal spectrum, mainly is used as the antimicrobial treatment of skin and mucosa.Itself is insoluble in water chlorhexidine, but the form of gluconic acid is water miscible.Antibacterial activity is to stick and destroy the cell serous coat, causes the cellular content precipitation.At once the antibacterial activity of chlorhexidine is slower than ethanol.It has good anti-gram positive bacteria effect, and is little to the Mycobacterium effect a little less than the effect to gram-negative bacteria and fungus, invalid to the brood cell.In vitro tests shows effective to virus such as herpesvirus, HIV, cytomegalovirus, influenza virus and RSV that film is arranged, but obviously lower to virus such as rotavirus, enterovirus and adenovirus effectiveness without film.
Quaternary ammonium salt is large class formation chemical compound complicated and changeable.Wherein alkyl Benzalkonii Chloridum (benzalkonium bromide) is widely used clinical.Other chemical compounds as disinfectant comprise Benzalkonii Chloridum, benzalkonium bromide.Once be widely used in the every field such as health care.Although the quaternary ammonium salt antibacterial bacteriostasis such as discovered in recent years benzalkonium bromide are very strong, fungicidal spectrum is narrower: the effect to gram-negative bacterias such as Pseudomonas aeruginosas is relatively poor, and is then substantially invalid to mycobacterium tuberculosis and bacterial spore; Only but the deactivation lipophilic is viral, and relatively poor to the hydrophilic viruses effect.
Triclosan is a kind of high-efficiency broad spectrum antibacterial.In the later development sixties in 20th century, be widely used in the consumer goodss such as soap, toothpaste, cleansing milk, handwashing liquid for medical personnel and public place.Antibiotic concentration is 0. 2%-2. 0%.The antibacterial activity of triclosan is to enter bacterial cell and affect cell serous coat and RNA, fatty acid and protein synthesis.Triclosan has certain antimicrobial spectrum, but is partial to antibacterial.Triclosan is better than gram negative bacilli to the effect of gram positive bacteria (comprising M RSA), particularly Pseudomonas aeruginosa.Triclosan has certain activity to Mycobacterium and Candida, but to the activity of filament fungus a little less than.Studies show that in a large number, lower than chlorhexidine, povidone iodine and alcohol product with the wash one's hands reduction number of rear bacterium colony of triclosan.
The reappraise safety of triclosan of U.S. FDA in 2011 and EPA comes from the product that contains triclosan in case react with chloride tap water, can form the material of a kind of being called as " chloroform ", i.e. chloroform.The chloroform animal experiment finds to have slight teratogenecity to heart and liver injury, can induce white mice generation hepatocarcinoma, but there is no human carcinogen's research data.For the sake of assurance, IARC and the U.S. all classify chloroform as the suspect carcinogen that human body is had potential threat.In clinical use, because abuse of antibiotics is carried out natural selection to the variation antibacterial, thereby produced " superbacteria ", it can make malignant bacteria incomparably powerful, resists all antibiotic.
Therefore; exploitation safety, wide spectrum, efficient, working concentration is low, environmental friendliness, user of service's skin is had the disinfectant of protection is the important of following clinical demand always; along with the increase of testing microorganism and disinfectant unsafe factor; requirement for experiment condition is increasingly strict, causes the new disinfectant development difficulty large, expensive many.Also be no lack of on the market the Chinese medicine extract of containing gel, adopt several kinds of Chinese medicinal materials composite more, the effect complicated, mechanism is indefinite.The invention provides a kind of definite ingredients, have the plant dry-cleaning disinfecting gel of excellent antibacterial effect.
Chitosan (chitosan) is to be obtained through deacetylation by the chitin (chitin) that nature extensively exists, chemical name is Chitosan (1-4)-2-amino-B-D glucose, from 1859, after Frenchman Rouget at first obtains chitosan, biological functionality and the compatibility of this natural polymer, blood compatibility, safety, the premium properties such as microbic resolvability are by all trades and professions extensive concern, at medicine, food, chemical industry, cosmetics, water treatment, METAL EXTRACTION and recovery, the applied research of the numerous areas such as biochemistry and biomedical engineering has been obtained major progress.The bacteriostatic activity chitosan of chitosan in the weak acid solvent, be easy to the dissolving, it is worthy of note especially the dissolving after solution in contain amino (NH2+), these amino are by coming anti-bacteria in conjunction with negatron.The Antibacterial activity of chitosan makes it have a wide range of applications in fields such as medicine, weaving and food.Chitosan and derivant thereof have preferably antibacterial activity, can suppress the growth and breeding of some funguses, antibacterial and virus.Think that at present its possible mechanisms has three: one, because the polycation of chitosan is easy to the group effect of fungal cell's surface band negative charge, thereby change flowability and the permeability of pathogen cell membrane; The 2nd, disturb copying and transcribing of DNA; The 3rd, the metabolism of blocking-up pathogen.In recent years, have many researcheres to propose chitosans by inducing pathogenesis-related proteins, the modes such as accumulation secondary metabolite and signal conduction reach the viewpoint of antibiotic purpose.
Chinese patent CN1309304C discloses a kind of disinfecting gel and its preparation method and application, and it comprises staphylococcus lysozyme 0.0001-0.01wt%, lysozyme 0.01-1wt%, thickening agent 1-20wt%, emollient 0.1-15wt%, water surplus.It can be used for the cleaning and disinfection of hand skin, but this disinfecting gel in use long-term killing effect is not good.Chinese patent application CN1498538A discloses a kind of marine organism sterilizing agent take chitin, alginic acid element, agaroidin gel sugar, DP300 DP300, cedar wood alcohol as effective ingredient, this disinfectant is non-stimulated, abnormal smells from the patient delicate fragrance, but its same existence is defective of virus killing poor effect for a long time, is difficult to adapt to the needs of prolonged operations.
Summary of the invention
Large in order to overcome the skin irritation that exists in the existing skin degerming process, the prior art defective that fungicidal spectrum is narrow and action time is short, the invention provides a kind of new skin degerming gel, skin degerming of the present invention is sterilized or skin degerming with the hands that gel can be used in disinfecting or the surgery preoperative disinfection, its bactericidal effect is excellent, effect is lasting, has good application prospect and economic benefit.
The present invention adopts following technical scheme to realize:
A kind of skin degerming gel of the present invention, its active component is comprised of chitosan, oligochitosan, baicalin, chlorhexidine, sodium alginate, agarose and Folium Menthae extract.
Wherein: chitosan is by β-(1,4) the glucosamine macromole natural polymer of glycosidic bond connection, it is the unique polysaccharide with the positive charge effect of occurring in nature, chitosan is by the entrained negative charge interaction of self entrained positive charge and microbial cell, destroy the bacterial cell wall construction, cause cell component to leak and play antibacterial action; To there not being the microorganism of cell membrane; similar flocculation occurs by penetrating in the cell with biomacromolecule with anion; upset the normal physiological function of cell; the blocking dna biosynthesis; thereby the Growth and Reproduction of anti-bacteria does not produce drug resistance, significantly improves Effects on local immunological functions, improves mucosa hyperemia, protects mucosa, promotes epithelial cell growth.
Oligochitosan also is Chitosan oligosaccharide, also claim chitin oligosaccharide, formal name used at school β-Isosorbide-5-Nitrae-oligosaccharide-glucamine, being a kind of brand-new product that chitosan is obtained through special biological enzyme technology processing, is the low molecular weight product that water solublity is better, function is large, biological activity is high.It has the unexistent higher solubility of chitosan and easily by the function of many uniquenesses such as organism absorption, and it act as 14 times of chitosan.Prevention or treatment are by bacterial Animal diseases such as staphylococcus aureus, Escherichia coli, Actinobacillus, streptococcus mutanses.But the disease resistance of inducing plant produces immunity and killing action to multiple fungus, antibacterial and virus.Antioxidation: can eliminate the human body oxygen-derived free radicals, the activation equipment somatic cell, slow down aging suppresses the skin surface harmful bacteria and grows, and performance of keeping humidity is excellent.
Baicalin (Baicalin) is to extract a kind of flavone compound separate from scutellariae,radix, has significant biological activity, has antibacterial, diuresis, antiinflammatory, resistance attitude and spasmolysis, and has the stronger physiological potencies such as antitumor response.Occupied critical role at clinical medicine.Baicalin can also absorbing ultraviolet, removes oxygen-derived free radicals, and therefore generation that again can check melanin both can be used for medicine, also can be used for cosmetics, was a kind of good functional cosmetics raw material.Be one of Radix Scutellariae antimicrobial component, contain the clinical trachoma that is used for the treatment of of collyrium of 3% baicalin, medicament benemicin is similar preferably to treatment trachoma for curative effect.
Chlorhexidine is white crystalline powder, is a kind of surfactant, has great broad-spectrum antibacterial, bactericidal action, and various bacteria is had effect, and externally used antimicrobial agent and cationic surfactant are to most gram positive bacterias and gram negative bacteria tool killing action.Except can be antibacterial, but also kill bacteria.The chlorhexidine sterilizing mechanisms mainly is to destroy permeability barrier on the antibacterial endochylema film (but not former hypothesis, ATP enzyme lose activity and reach cause bactericidal effect), and low concentration can cause part endochylema seepage, high concentration then can cause endochylema cohesion degeneration, thus sterilization.Its advantage be zest than other externally used antimicrobial agent be low, seldom anaphylactic reaction appears.Chlorhexidine toxicity is low, zest is little, non-corrosiveness, is applicable to disinfecting of skin mucosa and wound surface, is widely used in the sterilization of mucosa, skin, operation, wound surface, apparatus, apparatus etc.
The molecular formula of sodium alginate is C5H7O4COONa, and CAS number is 9005-38-3.Also can be described as sodium alginate, alginate jelly.A kind of macromolecular compound of high viscosity, molecular formula are (C6H7NaO6) n.Product is faint yellow or yellowish-brown ambiguity powder.Typical hydrophilic colloidal sol.Be widely used in the industries such as food, printing and dyeing, medicine, papermaking.Sodium alginate, a kind of natural polysaccharide has the required stability of pharmaceutical preparation adjuvant, dissolubility, viscosity and safety.1881, Englishize scholar E.C.Stanford at first carried out scientific research to the alginate extract in the brown seaweed.He finds that this alignic extract has the ability of concentrated solution, formation gel and film forming.
Agarose is linear polymer, basic structure be 1,3 β that links-D-gala furanose and Isosorbide-5-Nitrae link 3, the 6-α-L-gala furanose that dewaters.Agarose especially has significant steadiness, stagnates and spend and hysteresis quality, and easily absorb moisture because special gelling property is arranged, and special stabilizing effect is arranged; The fields such as edible, medical, chemical industry, weaving, national defence have been widely used in.In recent years because its good biocompatibility is widely used in again the production of bio-separation medium.
The present more application of Herba Menthae is to utilize its aromatic substances such as menthol to do medicine etc. as spice and effective component extracting.Research aspect Herba Menthae antibacterial mainly concentrates on Herba Menthae quintessence oil aspect.Studies show that the Herba Menthae quintessence oil has obvious inhibitory action to gram positive bacteria and gram negative bacteria, to the inhibitory action of fungus even be better than inhibitory action to antibacterial, also have the scholar to separate to have obtained the bacteriostatic active ingredients of Herba Menthae quintessence oil.Folium Menthae extract of the present invention both can become the Herba Menthae quintessence oil for mainly containing effect, also contained other the antipathogenic composition that extracts in the Herba Menthae, also can become for the peppermint essence line of oils is only arranged.Folium Menthae extract take the Herba Menthae quintessence oil as main effective ingredient in the prior art all can be used for the present invention.For clearer expression Folium Menthae extract of the present invention, the applicant provides a kind of preparation method for preparing above-mentioned Folium Menthae extract, its step comprises: get dry Herba Menthae, pulverize, sampling 10g is contained in the beaker, adds to extract solvent 120mL, with sodium hydroxide solution or hydrochloric acid solution the pH value of lixiviating solution is transferred to designated value, soak 16～24h.Solution colour becomes yellowish-brown or 4 layers of filtered through gauze of brown rear usefulness, gets 55mL filtrate and be distilled to 5mL with rotary evaporator under 50 ℃.Cold preservation, for subsequent use.
The present invention makes gel preparation with above-mentioned active component composition according to optimal proportion, through test of many times and clinical verification, can either broad-spectrum high efficacy kill common pathogenic flora, reduce the clinical infection probability has good histocompatibility simultaneously, and skin irritation is little.Four kinds of drug components share, and have significant synergism aspect the common pathogen killing, and stop sticking, breed of pathogenic bacterium, have also avoided using the drug resistance, superinfection, anaphylaxis of antibiotic initiation and to the drawbacks such as toxic and side effects of body.
The inventor explores constituent content in the above-mentioned gel as evaluation criterion the killing effects of pathogenic bacterium take above-mentioned gel, the inventor finds that component weight percentage is in above-mentioned gel: chitosan 1.5%-6%, oligochitosan 1.5-10%, baicalin 2%-8%, chlorhexidine 0.5%～2%, sodium alginate are 0.5%～1.5%, agarose 2%-5%, Folium Menthae extract is 1.5%-5%, surplus is water, and described gel is better to the killing effect of pathogenic bacterium.Wherein work as gel and contain chitosan 3.5%, oligochitosan 6%, baicalin 5%, chlorhexidine 1.4%, sodium alginate are 1%, agarose 3%, and when Folium Menthae extract was 3.5%, its killing effect to pathogenic bacterium was best.
Gel of the present invention can also contain wetting agent, thickening agent and pH regulator except containing active component.All can be used for gel of the present invention about wetting agent, thickening agent and pH regulator in the prior art.Wherein said wetting agent is preferably glycerol.Described thickening agent is preferably carbomer, specifically can select carbomer 934, Acritamer 940 or the Carbopol 941 of different viscosities.Described pH regulator is preferably triethanolamine, triisopropanolamine, diisopropanolamine (DIPA) or its combination.The pH of described gel is preferably 5.0-6.5.
Gel preparation of the present invention can be widely used in the skin degerming field, and its mechanism of action is:
A) chitosan is macromolecule polyalcohol, and the strand disperse is stretched, and is great swelling state, and has viscosity; Sodium alginate has stronger biocidal property and film property.Disinfecting gel agent of the present invention can form the gelatinous mass of the thickness of clarification, and gel is attached to skin surface and forms protecting film, and sterilization face is isolated from the outside, and avoids the secondary cross infection.
B) this gel is subacidity, be not subjected to microbiological effect, particularly multiple bacteriostatic active ingredients coupling in the gel of the present invention, not only can enlarge the anti-pathogenic bacterium spectrum of gel, and can aspect antibacterial activity, have significant collaborative reinforced effects, thereby suppress the pathogen breeding, strengthen diseased region to resistivity and the immunocompetence of pathogenic bacteria, virus.
C) characteristics of the equal tool water-soluble base of chitosan in performance subjective antisepsis activity, can slowly discharge parcel chlorhexidine, baicalin and Folium Menthae extract wherein, prolong the virus killing effect of compositions.
Based on this, the invention provides a kind of purposes of above-mentioned gel, namely above-mentioned gel is used in hands sterilization or skin degerming, and wherein said hands sterilization or skin degerming can be disinfecting or surgery preoperative disinfection.The embodiment of the invention 7 shows that disinfecting gel agent of the present invention all has stronger killing action to most pathogenic bacterium certificate.The embodiment of the invention 8 shows that disinfecting gel of the present invention can effectively kill staphylococcus aureus (Staphyloccocus aureus), pseudomonas aeruginosa (Pseudomonas aeruginosa), escherichia coli (Escherichia coli) and Candida albicans (Candida albicans) etc., and the sterilization 15min after and 200min after suppression ratio all reach more than 98.5%, be better than reference embodiment 1 and reference embodiment 2.On disinfecting time, disinfecting gel agent of the present invention is more lasting action time, and the bacterium colony degree of killing of 15min and 200min is more or less the same, and the bacterium colony killing rate of reference embodiment 1 and reference embodiment 2 is along with the prolongation of disinfecting time fast reducing then.Disinfecting gel agent of the present invention long operation preferably is used.The embodiment of the invention 9 experimental results show that repeatedly medication is very little to skin irritation in disinfecting gel agent of the present invention, with the matched group there was no significant difference.
The using method of gel of the present invention is: 1) surgery preoperative disinfection: after washing one's hands with soap lye by standard, flowing water washes down, and dries with sterile towel, then gets this product stock solution and evenly is applied to hands and forethiga one time, about 5 ~ the 10ml of every hands arm acts on 3 minutes both hands rubbings to doing.2) disinfecting of hands: get this product 3ml and be applied to 1 minute rubbing of hand effect to doing.3) skin health sterilization: get this product and be applied in right amount or be sprayed on skin surface to moistening, act on 3 minutes natural dryings.
When gel of the present invention is used for hands sterilization or skin degerming, compared with prior art have following technical advantage:
1) gel that makes by rational formula of the present invention is by the multiple bacteriostatic active ingredients of coupling, each component obtains remarkable synergism aspect antibacterial when significantly enlarging antimicrobial spectrum, thereby suppress the pathogen breeding, strengthen diseased region to resistivity and the immunocompetence of pathogenic bacteria, virus.
2) active component mainly comes from nature in the gel of the present invention, good biocompatibility, and side effect is low, the drug resistance of having avoided using antibiotic to cause, superinfection, anaphylaxis and to the drawbacks such as toxic and side effects of body.
3)When gel of the present invention was used for colpitis and the reparation of skin skin ulcer face, its medicine irritation was little, and bactericidal activity is lasting, can significantly improve the therapeutic effect of medical and nursing work person's compliance and medicine, greatly reduces postoperative infection.
The specific embodiment
Below further describe the present invention by the specific embodiment, but the present invention is not limited only to following examples.Within the scope of the invention or within not breaking away from content of the present invention, spirit and scope, sanitizing composition of the present invention is suitably improved, replaces the identical component of effect, will become apparent to those skilled in the art that they all are deemed to be included within the scope of the present invention.
Embodiment 1: skin degerming gel of the present invention
A kind of for the skin degerming gel, active component comprises chitosan, oligochitosan, chlorhexidine, baicalin, sodium alginate, agarose, Folium Menthae extract.Wherein chitosan concentration is 3%, oligochitosan concentration is 4%, chlorhexidine concentration is 1.2%, Determination of baicalin is 3.7%, sodium alginate concentration is 1%, agarose concentration is 2.5%, Folium Menthae extract 3%.
To prepare 1000 gram gels as example, contain 30g chitosan, 40g oligochitosan, 12g chlorhexidine, 37g baicalin, 10g sodium alginate, 25g agarose in every kilogram of gel, the 30g Folium Menthae extract.Concrete preparation method is that 30g chitosan, 25g agarose and 40g oligochitosan are added in 816 milliliters of purified water, with high speed dispersor it is fully dissolved; While is well-dispersed in the 12g chlorhexidine in 50 milliliters of propylene glycol, scattered chlorhexidine and adds in the solution of above-mentioned abundant dissolving, continues to stir to make mix homogeneously, and the pH value of solution is between 5.0 to 6.0, and product becomes gel; Use at last the aqueous solution of 0.22um microporous filter membrane aseptic filtration sodium alginate and the aqueous solution of Folium Menthae extract, add in the gel according to the content in every kilogram of gel again, add at last wetting agent, thickening agent and pH adjusting agent and namely can be made into finished product.
Embodiment 2-6 skin degerming gel of the present invention
The skin antiseptic prescription is as shown in table 1, and preparation method is with embodiment 1 skin degerming gel preparation method.
The percentage ratio of table 1 embodiment 2-embodiment 6 skin degerming gels forms
The In Vitro Bacteriostasis experiment of embodiment 7 sanitizing compositions of the present invention
This tests employed strain and culture medium is respectively α-hemolytic streptococcus (32209), beta hemolytic streptococcus (32210), colon bacillus (44102), proteus vulgaris (49101), pseudomonas aeruginosa (Pseudomonas aeruginosa), Candida albicans (Candida albicans) Salmonella paratyphi A (50001), shigella dysenteriae (51252), staphylococcus aureus [26111 (5)], hemophilus influenza (58529-4), nutrient agar (in be stranded pharmaceutical biological product calibrating institute); Opacity tube (Chinese bacterial turbidity standard, the biological combinations calibrating of Chinese medicine institute).
In Vitro Bacteriostasis experiment: 1. prepare the pastille agar plate.Tested material is pressed the culture medium that doubling dilution is prepared different pharmaceutical concentration, heating, modulation is required pH value separately, and 121 ℃ of sterilization 30 min are chilled to 45 ℃.Pour into into aseptic flat board (plate d=65 mm, 10 ml/ plates), finally make the extension rate that contains tested material stock solution (2g/ml) in the culture medium be respectively l:2,1:4 ... 1:1024.Do simultaneously blank culture medium separately.2. inoculation.Get the bacterium in young age that reaches the third generation, be the bacterium liquid to be checked of 108 cfu/ml by standard opacity tube corrected concentrations with 0.9% sodium chloride solution respectively, be inoculated in respectively the separately pastille agar surface of different doubling dilutions, inoculate simultaneously parallel point 3 points, make contain 2 bacterium liquid at every.The liquid-drop diameter of each vaccination is 5-8 mm.After the vaccination drying, upset is dull and stereotyped cultivates 20 h observed results (doing simultaneously blank) in 36 ℃ of constant incubators.3. criterion.Minimum dilution tested material concentration on the agar plate that bacterium colony do not occur is as its minimum inhibitory concentration (MIC).
The concentration of disinfecting gel agent is that sterilize in disinfecting gel agent chitosan, oligochitosan, baicalin, chlorhexidine, sodium alginate, agarose and Folium Menthae extract is as shown in table 2 to the minimal inhibitory concentration of each pathogenic strain in this experiment:
Table 2 sanitizing composition of the present invention is to the minimal inhibitory concentration of pathogenic strain
The result shows that disinfecting gel agent of the present invention all has stronger killing action to most pathogenic bacterium certificate,
Reference embodiment 1 adopts the disinfecting gel of CN1309304C embodiment 1
Preparation method is: the content 0.01g(0.01 wt% that at first takes by weighing staphylococcus lysozyme), the content 0.3g(0.3 wt% of lysozyme), the content 3g(3wt% of hydroxy propyl cellulose), glycerol 5g(5wt%), surplus is water, and mix homogeneously can obtain required disinfecting gel.
Reference embodiment 2 adopts among the Chinese patent application CN1498538A and fills a prescription three, and its prescription consists of chitin 3.0-6.0, alginic acid element 2.0-5.0, agaroidin gel sugar 1.0-2.0, DP300 DP300 0.5-0.8, cedar wood alcohol 1.8-2.0, purified water adds to 100.
The Disinfection Effect of embodiment 8 disinfecting gel agent of the present invention relatively
Use bacterium liquid to be measured evenly to be coated onto medical apparatus surface, and get one (0.05ml) bacterium liquid to be measured and put into culture medium flat plate and cultivate, coat rapidly disinfecting gel agent of the present invention on the apparatus surface.Respectively at placing flat board to cultivate from apparatus surface scraping 1 (0.05ml) behind the 15min and behind the 200min.Each bacterium liquid is cultivated respectively 48 hours respectively countings at flat board under each bacterium preference temperature.Calculating is coated with the bacterium colony killing rate behind the 15min and 200min behind the gel.
The Disinfection Effect of table 3 sanitizing composition of the present invention relatively
Disinfecting gel free from admixture of the present invention, without particulate matter.As can be seen from Table 3, it can effectively kill staphylococcus aureus (Staphyloccocus aureus), pseudomonas aeruginosa (Pseudomonas aeruginosa), escherichia coli (Escherichia coli) and Candida albicans (Candida albicans) etc., and the sterilization 15min after and 200min after suppression ratio all reach more than 98.5%, be better than reference embodiment 1 and reference embodiment 2.On disinfecting time, disinfecting gel agent of the present invention is more lasting action time, and the bacterium colony degree of killing of 15min and 200min is more or less the same, and the bacterium colony killing rate of reference embodiment 1 and reference embodiment 2 is along with the prolongation of disinfecting time fast reducing then.Disinfecting gel agent of the present invention long operation preferably is used.
The repeatedly irritation test of embodiment 9 sanitizing compositions of the present invention
Laboratory animal selects regular grade white New Zealand to plant 4 of rabbit, 24h shaves off back part of animal spinal column diamond wool before the test, shave a mao scope left and right sides Ge Yue 3cm * 3cm, the disinfecting gel agent of the present invention that during test tested material 0.5ml(is prepared by the embodiment of the invention 3) evenly be applied on the 2.5cm * 2.5cm skin of left side, the right side compares with distilled water.Smear every day once, continuously 14d.From second day, smear front unhairing every day, clean with warm water, observe dermoreaction behind the 1h and carry out skin irritation reaction scoring, after the off-test, calculate respectively 14 days total marks, 14 days every animal integral mean values, every day, every animal integral mean value and carried out the skin irritation strength grading.Repeatedly the irritation test result is as shown in table 4 in disinfecting gel agent of the present invention.
Table 4 sanitizing composition of the present invention is the irritation test result repeatedly
Show that by table 3 experimental result repeatedly medication is very little to skin irritation in disinfecting gel agent of the present invention, with the matched group there was no significant difference.
Claims (10)
1. skin degerming gel, it is characterized in that: its active component is comprised of chitosan, oligochitosan, baicalin, chlorhexidine, sodium alginate, agarose and Folium Menthae extract.
2. skin degerming gel as claimed in claim 1, it is characterized in that: described each component weight percentage of gel active component is: chitosan 1.5%-6%, oligochitosan 1.5-10%, baicalin 2%-8%, chlorhexidine 0.5%～2%, sodium alginate are 0.5%～1.5%, agarose 2%-5%, Folium Menthae extract are 1.5%-5%.
3. skin degerming gel as claimed in claim 2, it is characterized in that: described each component weight percentage of gel active component is: chitosan 3.5%, oligochitosan 6%, baicalin 5%, chlorhexidine 1.4%, sodium alginate are 1%, and agarose 3%, Folium Menthae extract are 3.5%.
4. such as the arbitrary described skin degerming gel of claim 1-3, it is characterized in that: Folium Menthae extract is the compositions that comprises the Herba Menthae quintessence oil in the described gel, the preparation method of described Folium Menthae extract comprises the steps: to get dry Herba Menthae, pulverize, sampling 10g is contained in the beaker, adds to extract solvent 120mL, with sodium hydroxide solution or hydrochloric acid solution the pH value of lixiviating solution is transferred to designated value, soak 16～24h; Solution colour becomes yellowish-brown or 4 layers of filtered through gauze of brown rear usefulness, and get 55mL filtrate and under 50 ℃, be distilled to 5mL with rotary evaporator, cold preservation, and get final product.
5. skin degerming gel as claimed in claim 4, it is characterized in that: Folium Menthae extract is the Herba Menthae quintessence oil in the described gel.
6. such as the arbitrary described skin degerming gel of claim 1-3, it is characterized in that: described gel contains wetting agent, thickening agent and pH regulator.
7. skin degerming gel as claimed in claim 6, it is characterized in that: wetting agent is glycerol in the described gel, described thickening agent is carbomer, and described pH regulator is one or more the combination in triethanolamine, triisopropanolamine, the diisopropanolamine (DIPA).
8. such as the arbitrary described skin degerming gel of claim 1-3, it is characterized in that: the pH of described gel is 5.0-6.5.
9. the arbitrary described skin degerming gel of claim 1-3, the application in hands sterilization or skin degerming.
10. skin degerming gel as claimed in claim 9 is characterized in that: described hands sterilization or skin degerming are disinfecting or surgery preoperative disinfection.
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Cited By (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105250513A (en) * | 2015-11-19 | 2016-01-20 | 朱坤福 | Skin bactericidal solution and preparation method thereof |
| WO2016059653A1 (en) * | 2014-10-13 | 2016-04-21 | Nex Medical Antiseptics S.R.L. | Blue dyed chlorhexidine antimicrobial composition for skin disinfection |
| CN105997648A (en) * | 2016-06-21 | 2016-10-12 | 江苏奇力康皮肤药业有限公司 | Skin protection gel containing natural extracts |
| CN106963977A (en) * | 2017-05-26 | 2017-07-21 | 广东海洋大学 | A kind of Breviscapinun/chitosan composite aquogel for suppressing cicatrization and preparation method thereof |
| CN107158272A (en) * | 2017-06-08 | 2017-09-15 | 苏州凌科特新材料有限公司 | A kind of anti-inflammation medical gel agent and preparation method thereof |
| CN107158273A (en) * | 2017-06-08 | 2017-09-15 | 苏州凌科特新材料有限公司 | Medical disinfection gel and preparation method thereof |
| CN107530468A (en) * | 2015-03-10 | 2018-01-02 | 科发龙技术公司 | The local method for reducing microbial skin flora |
| CN107970259A (en) * | 2017-12-19 | 2018-05-01 | 魏立梅 | A kind of medical composition and its use for treating acute pulpitis |
| CN108553337A (en) * | 2018-04-27 | 2018-09-21 | 普宁康特生物科技有限公司 | Special type resists antibacterial thimerosal |
| CN108815381A (en) * | 2018-06-13 | 2018-11-16 | 上海长海医院 | It is a kind of for the Chinese medicine metatitanic acid silver composite porous material and preparation method of burn wound healing and application |
| CN109908066A (en) * | 2019-04-04 | 2019-06-21 | 派能生物科技（深圳）有限公司 | A kind of preparation method of the composition for skin repair |
| WO2020043665A1 (en) | 2018-08-27 | 2020-03-05 | Claudia Eder | Antiseptic gel |
| CN114831924A (en) * | 2021-03-17 | 2022-08-02 | 武汉恒信源药业有限公司 | Urotropine and vaginal local administration preparation of compound thereof and application |
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Cited By (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2016059653A1 (en) * | 2014-10-13 | 2016-04-21 | Nex Medical Antiseptics S.R.L. | Blue dyed chlorhexidine antimicrobial composition for skin disinfection |
| CN107530468A (en) * | 2015-03-10 | 2018-01-02 | 科发龙技术公司 | The local method for reducing microbial skin flora |
| CN105250513A (en) * | 2015-11-19 | 2016-01-20 | 朱坤福 | Skin bactericidal solution and preparation method thereof |
| CN105997648A (en) * | 2016-06-21 | 2016-10-12 | 江苏奇力康皮肤药业有限公司 | Skin protection gel containing natural extracts |
| CN106963977A (en) * | 2017-05-26 | 2017-07-21 | 广东海洋大学 | A kind of Breviscapinun/chitosan composite aquogel for suppressing cicatrization and preparation method thereof |
| CN106963977B (en) * | 2017-05-26 | 2018-03-30 | 广东海洋大学 | A kind of Breviscapinun/chitosan composite aquogel for suppressing cicatrization and preparation method thereof |
| CN107158273A (en) * | 2017-06-08 | 2017-09-15 | 苏州凌科特新材料有限公司 | Medical disinfection gel and preparation method thereof |
| CN107158272A (en) * | 2017-06-08 | 2017-09-15 | 苏州凌科特新材料有限公司 | A kind of anti-inflammation medical gel agent and preparation method thereof |
| CN107970259A (en) * | 2017-12-19 | 2018-05-01 | 魏立梅 | A kind of medical composition and its use for treating acute pulpitis |
| CN108553337A (en) * | 2018-04-27 | 2018-09-21 | 普宁康特生物科技有限公司 | Special type resists antibacterial thimerosal |
| CN108815381A (en) * | 2018-06-13 | 2018-11-16 | 上海长海医院 | It is a kind of for the Chinese medicine metatitanic acid silver composite porous material and preparation method of burn wound healing and application |
| WO2020043665A1 (en) | 2018-08-27 | 2020-03-05 | Claudia Eder | Antiseptic gel |
| CN109908066A (en) * | 2019-04-04 | 2019-06-21 | 派能生物科技（深圳）有限公司 | A kind of preparation method of the composition for skin repair |
| CN109908066B (en) * | 2019-04-04 | 2022-01-21 | 合肥贝壳派创新科技有限公司 | Preparation method of composition for skin repair |
| CN114831924A (en) * | 2021-03-17 | 2022-08-02 | 武汉恒信源药业有限公司 | Urotropine and vaginal local administration preparation of compound thereof and application |
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| CN103356738B (en) | 2015-05-20 |
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Inventor after: Wang Weijuan Inventor after: Jiao Nianqiang Inventor after: Liu Zhongwei Inventor after: Du Guangqing Inventor before: Jiao Nianqiang Inventor before: Liu Zhongwei Inventor before: Du Guangqing |
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Application publication date: 20131023 Assignee: Shandong ocean Biotechnology Co., Ltd. Assignor: Rizhao Zhongsheng Marine Biotechnology Co., Ltd. Contract record no.: 2015370000088 Denomination of invention: Skin disinfection gel and its application License type: Exclusive License Record date: 20150511 |
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