CN111728935A

CN111728935A - Probiotic-containing micro-ecological particle and preparation method and application thereof

Info

Publication number: CN111728935A
Application number: CN202010649662.7A
Authority: CN
Inventors: 陆锦平; 丛琳; 罗嵘; 陈晓朋; 李雪竹
Original assignee: Aussia Australia Ltd; Guangzhou Ousia Industrial Co ltd
Current assignee: Aussia Australia Ltd; Guangzhou Ousia Industrial Co ltd
Priority date: 2020-07-08
Filing date: 2020-07-08
Publication date: 2020-10-02

Abstract

The invention belongs to the field of skin care products, and particularly relates to probiotics-containing micro-ecological particles and a preparation method and application thereof. The probiotic-containing microecological particle at least comprises the following components in parts by weight: 60-90 parts of deionized water, 5-20 parts of oily components, 0.2-3 parts of probiotic composition and 1-5 parts of surfactant. The probiotics-containing microecological particle provided by the invention can accelerate the peeling of old cutin of skin, promote the growth of new cells, regulate the microecological balance of skin, maintain the microecological balance of skin flora for a longer time, improve skin barrier and achieve the effects of long-acting moisture preservation and the like, can be directly added into cosmetics of different formulations such as astringent, essence, emulsion and the like, and effectively exert various effect characteristics of probiotics; the probiotic-containing microecological particles prepared by the invention have uniform particle size, the average particle size of 0.1-1mm and good stability.

Description

Probiotic-containing micro-ecological particle and preparation method and application thereof

Technical Field

The invention belongs to the field of skin care products, and particularly relates to probiotics-containing micro-ecological particles and a preparation method and application thereof.

Background

The skin is constantly in contact with the environment, and the stratum corneum is constantly challenged by the environment, such as temperature, humidity, pathogens, pollution, sunlight, ultraviolet rays, and the like. If the skin is not protected, the stratum corneum is lack of water, the growth environment of the microbial flora of the skin is destroyed, the number of the microbial flora is reduced, and then various problems of barrier damage occur.

On a normal skin surface, a large number of microorganisms, including microbial populations such as bacteria, fungi and viruses, are present, forming a mutually restricted and balanced microbial population. Normally, microorganisms are present orderly on the skin surface, and not only the microecological environment on the skin surface is maintained stable, but also bioactive substances used for the skin are secreted and produced. The probiotic is used in cosmetics, and can balance skin epidermal flora and repair skin barrier. The probiotic bacteria can be used for treating atopic dermatitis by external application, namely inhibiting the proliferation of other bacteria to balance the microbial flora on the skin surface. Meanwhile, the probiotics can also reduce the release of inflammatory mediators and promote the repair of skin barriers by external application.

Currently, skin care products containing probiotic metabolites are in the market, but the effects of most commercial products are only limited in the publicity level. Some commodities are added with natural extracts, but the probiotic metabolite solution and other extracts are simply mixed, so that long-acting moistening and water replenishing cannot be realized, long-acting moistening effects cannot be realized, and the stability of the natural extracts and the like at high temperature and under illumination is poor.

Disclosure of Invention

In order to solve the technical problem, the first aspect of the present invention provides a probiotic-containing microecological particle, which comprises the following components in parts by weight: 60-90 parts of deionized water, 5-20 parts of oily components, 0.2-3 parts of probiotic composition and 1-5 parts of surfactant; (ii) a The oily component comprises solid oil component and liquid oil component; the mass ratio of the solid oil component to the liquid oil component is 1: (2-4).

As a preferable technical scheme, the average grain diameter of the probiotics-containing micro-ecological particles is 0.1-1 mm.

As a preferred technical scheme, the probiotic composition is selected from one or more of La Flora EC-12, repairComplex CLR PF and Fermentage ChardonnaY BG.

As a preferred embodiment, the oily component includes a solid oil component and a liquid oil component; the liquid oil component comprises silicone oil and other liquid oil components.

As a preferred solution, the other liquid oil component is selected from one or more of olive oil, sweet almond oil, grape seed oil, glycerol tri (ethyl hexanoate), squalane, pentaerythritol tetraisostearate, pentaerythritol tetra (ethyl hexanoate), caprylic/capric triglyceride, isononyl isononanoate, cetyl ethyl hexanoate.

As a preferred technical scheme, the HLB value of the surfactant is 2-5.

As a preferable technical scheme, the component containing the probiotic micro-ecological particles also comprises 0.01 to 0.05 weight part of thickening agent.

As a preferable technical scheme, the thickening agent is selected from one or more of carbomer, xanthan gum, hydroxyethyl cellulose, triethanolamine, sodium polyacrylate starch, sodium alginate, acrylic acid (ester)/C10-30 alkanol acrylate cross-linked polymer and locust bean gum.

The second aspect of the present invention provides a method for preparing the probiotic-containing micro-ecological particle, which at least comprises the following steps:

(1) heating deionized water;

(2) adding the thickening agent into silicone oil, homogenizing and dispersing, adding the mixture into a water phase, and stirring;

(3 mixing the rest oily components except the silicone oil and the surfactant, dissolving at 65-90 ℃, adding the probiotic composition, stirring, and transferring into a water phase;

(4) cooling to 30-50 ℃, stopping stirring, and standing to obtain the probiotic-containing micro-ecological particles.

According to a third aspect of the invention, the probiotic-containing micro-ecological particles are applied to cosmetics, wherein the cosmetics are selected from one or more of face cream, facial mask, essence, toner, emulsion, facial cleanser, moisturizing liquid and skin base liquid.

Has the advantages that: the probiotics-containing micro-ecological particle provided by the invention is coated in the oil phase particle by adopting a particle coating manufacturing technology, the probiotics contained in the particle can accelerate the peeling of old cutin of skin, promote the growth of new cells and regulate the micro-ecological balance of the skin, and meanwhile, the particle acts on the skin, compared with the direct action of the existing probiotics, the micro-ecological particle containing the probiotics can maintain the micro-ecological balance of skin flora for a longer time, can improve skin barrier and achieve the effects of long-acting moisture retention and the like, can be directly added into cosmetics of different formulations such as astringent, essence, emulsion and the like, and effectively exerts various effect characteristics of the probiotics; the probiotic-containing microecological particles prepared by the invention have uniform particle size, average particle size of 0.1-1mm and good stability, and do not have the phenomena of particle deformation or trace oil production and the like after being stored for 1 week, 2 weeks and 1 month at normal temperature, 45 ℃ and-10 ℃ under the illumination condition.

Detailed Description

The technical features of the technical solutions provided by the present invention are further clearly and completely described below with reference to the specific embodiments, and the scope of protection is not limited thereto.

The term "prepared from …" as used herein is synonymous with "comprising". The terms "comprises," "comprising," "includes," "including," "has," "having," "contains," "containing," or any other variation thereof, as used herein, are intended to cover a non-exclusive inclusion. For example, a composition, process, method, article, or apparatus that comprises a list of elements is not necessarily limited to only those elements but may include other elements not expressly listed or inherent to such composition, process, method, article, or apparatus.

The conjunction "consisting of …" excludes any unspecified elements, steps or components. If used in a claim, the phrase is intended to claim as closed, meaning that it does not contain materials other than those described, except for the conventional impurities associated therewith. When the phrase "consisting of …" appears in a clause of the subject matter of the claims rather than immediately after the subject matter, it defines only the elements described in the clause; other elements are not excluded from the claims as a whole.

When an amount, concentration, or other value or parameter is expressed as a range, preferred range, or as a range of upper preferable values and lower preferable values, this is to be understood as specifically disclosing all ranges formed from any pair of any upper range limit or preferred value and any lower range limit or preferred value, regardless of whether ranges are separately disclosed. For example, when the range "1 to 5" is disclosed, the described range should be construed as including the ranges "1 to 4", "1 to 3", "1 to 2", "2 to 3" and "3 to 4", "4 to 5" and "3 to 5", etc. When a range of values is described herein, unless otherwise stated, the range is intended to include the endpoints thereof and all integers and fractions within the range.

The singular forms "a", "an" and "the" include plural referents unless the context clearly dictates otherwise. "optional" or "any" means that the subsequently described event or events may or may not occur, and that the description includes instances where the event occurs and instances where it does not.

In addition, the indefinite articles "a" and "an" preceding an element or component of the invention are not intended to limit the number requirement (i.e., the number of occurrences) of the element or component. Thus, "a" or "an" should be read to include one or at least one, and the singular form of an element or component also includes the plural unless the stated number clearly indicates that the singular form is intended.

In order to solve the technical problem, the invention provides a probiotic-containing microecological particle, which comprises the following components in parts by weight: 60-90 parts of deionized water, 5-20 parts of oily components, 0.2-3 parts of probiotic composition and 1-5 parts of surfactant.

In one embodiment, the probiotic micro-ecological particle-containing component comprises at least, in parts by weight: 75 parts of deionized water, 13 parts of oily components, 1.6 parts of probiotic composition and 3 parts of surfactant.

In one embodiment, the probiotic-containing microecological particles have an average particle size of 0.1 to 1 mm.

Silicone oil

In one embodiment, the silicone oil is selected from one or more of cyclopentadimethylsiloxane, polydimethylsiloxane, and octylmethicone.

Preferably, the silicone oil is polydimethylsiloxane.

In the present invention, the Polydimethylsiloxane (Polydimethylsiloxane) is a hydrophobic silicone material. The dimethyl silicone oil in the chemical state of the polydimethylsiloxane is colorless or light yellow liquid, is tasteless, has high transparency, has heat resistance, cold resistance, small viscosity change along with temperature, is waterproof, has small surface tension and has thermal conductivity.

In one embodiment, the polydimethylsiloxane is available under the brand KF-96A-6T, purchased from the Japan shin-Etsu group.

Oily component

In one embodiment, the oily component comprises a solid oil and a liquid oil.

In one embodiment, the mass ratio of the solid oil component to the liquid oil component is 1: (2-4).

Preferably, the mass ratio of the solid oil component to the liquid oil component is 1: 3.

(solid oil component)

In one embodiment, the solid oil component is selected from one or more of cetyl palmitate, ethylene glycol distearate, polyglycerol-3 beeswax, candelilla wax, cetyl alcohol, cetostearyl alcohol, behenyl alcohol, batyl alcohol, jojoba esters.

Preferably, the solid oil component is a mixture of behenyl alcohol and batyl alcohol.

In one embodiment, the mass ratio of behenyl alcohol to batyl alcohol is (2.5-3.5): 1.

preferably, the mass ratio of the behenyl alcohol to the batyl alcohol is 3.1: 1.

in the invention, the behenyl alcohol is a chemical substance with a molecular formula of C₂₂H₄₆O, molecular weight 326.6, CAS 661-19-8, is a solid skin-moistening and fat-imparting agent, and has excellent viscosity stabilizer for moistening skin, and making skin smooth.

In the present invention, the batyl alcohol is isolated from shark liver oil, and is also present in animal yellow bone marrow. Is an inherent substance in animal body, has high content in bone marrow hematopoietic tissue, may be one of hematopoietic factors in vivo, can increase the number of megakaryocytes and granulocytes reduced by radiation, and can prolong life time. Has effects in promoting leukocyte proliferation and resisting radiation, preventing and treating leukopenia, and resisting hematopoietic system inhibition caused by benzene poisoning and cytotoxic drugs, and the CAS number of batyl alcohol is 544-62-7.

(liquid oil component)

In one embodiment, the liquid oil component includes silicone oil and other liquid oil components.

Preferably, the silicone oil is polydimethylsiloxane.

In the present invention, the other liquid oil component means a remaining liquid oil component other than the silicone oil.

In one embodiment, the other liquid oil component is selected from one or more of olive oil, sweet almond oil, grape seed oil, glycerol tri (ethylhexanoate), squalane, pentaerythritol tetraisostearate, pentaerythritol tetra (ethylhexanoate), caprylic/capric triglyceride, isononyl isononanoate, cetyl ethylhexanoate.

Preferably, the other liquid oil is a mixture of squalane and pentaerythritol tetraisostearate.

In one embodiment, the mass ratio of squalane to pentaerythritol tetraisostearate is 1: (1.1-1.5).

Preferably, the mass ratio of the squalane to the pentaerythritol tetraisostearate is 1: 1.3.

in the invention, the squalane is hydrocarbon oil prepared by hydrogenating squalene extracted from deep sea shark liver, so the squalane is also called deep sea shark liver oil. The CAS number of the squalane is 111-01-3; the Squalane (Squalane) can strengthen and repair the epidermis, effectively form a natural protective film and help the balance between the skin and the sebum; squalane is a lipid closest to sebum of a human body, has strong affinity, can be integrated with the sebum membrane of the human body, and forms a natural barrier on the skin surface; squalane can also inhibit peroxidation of skin lipid, can effectively permeate into skin, can promote proliferation of skin basal cells, and has obvious physiological effects of delaying skin aging, improving and eliminating chloasma; squalane also can open skin pores, promote blood microcirculation, promote cell metabolism, and help repair damaged cells.

In the invention, the pentaerythritol tetraisostearate is liquid grease with large molecular weight, and the molecular formula is C₇₇H₁₄₈O₈The hair conditioner has the molecular weight of 1201.99 and the CAS number of 62125-22-8, is insoluble in water, provides moist and smooth hand feeling for hair, and provides very moist and non-greasy skin feeling for skin. Can be widely used in cream, lotion, shampoo, hair conditioner, topical ointment, etc.

In one embodiment, the liquid oil is a mixture of polydimethylsiloxane, squalane, pentaerythritol tetraisostearate.

In one embodiment, the mass ratio of polydimethylsiloxane, squalane, pentaerythritol tetraisostearate is (0.5-0.9): 1: (1.1-1.5).

Preferably, the mass ratio of the polydimethylsiloxane to the squalane to the pentaerythritol tetraisostearate is 0.7: 1: 1.3.

probiotic composition

In one embodiment, the probiotic composition is selected from one or more of La Flora EC-12, Repair ComplexCLR PF, and nutrient ChardonnaY BG.

Preferably, the probiotic composition is a mixture of La Flora EC-12, Repair Complex CLR PF, and nutrient Chardonnay BG.

In one embodiment, the La Flora EC-12, Repair complete CLR PF, and FermentageChardonnaY BG are in a mass ratio of 1: (8-12): (4-8).

Preferably, the mass ratio of the La Flora EC-12, the Repair Complex CLR PF and the nutrient ChardonnaYBG is 1: 10: 6.

in the present invention, the La Flora EC-12 is a lactic acid bacteria fermentation product, purchased from Nippon mono Bow.

In the present invention, the Repair complete CLR PF is a split yeast fermentation lysate, purchased from CLR, Germany.

In the present invention, the nutrient Chardonnay BG comprises fermented grape extract, glutathione, and lactobacillus, and is purchased from Nippon mono Bolus.

Surface active agent

In one embodiment, the surfactant is selected from one or more of a betaine type surfactant, an alkyl glucoside type surfactant, and a surfactant containing a stearate type structure.

In one embodiment, the betaine-type surfactant is selected from one or more of lauramidopropyl betaine, cocamidopropyl betaine, stearamidopropyl betaine, lauryl hydroxypropyl phosphate betaine, and myristyl dimethyl hydroxypropyl phosphate betaine.

In one embodiment, the alkyl glucoside type surfactant is selected from one or more of octyl glucoside, decyl glucoside, dodecyl glucoside, tetradecyl glucoside, hexadecyl glucoside, and octadecyl glucoside.

In one embodiment, the surfactant containing a stearate structure is selected from one or more of polyglyceryl 2-isostearate, polyglyceryl 3-diisostearate, polyglyceryl-10 stearate, glyceryl isostearate, glyceryl diisostearate, PEG-20 glyceryl triisostearate, and sorbitan isostearate.

In a preferred embodiment, the surfactant is a surfactant containing a stearate structure.

In a preferred embodiment, the surfactant has an HLB value of from 2 to 5.

The HLB value is as follows: the surfactant is an amphiphilic molecule with a hydrophilic group and a lipophilic group, and the amount of the size and the force balance degree between the hydrophilic group and the lipophilic group in the surfactant molecule is defined as the hydrophilic-lipophilic balance value of the surfactant.

In a more preferred embodiment, the surfactant is glycerol diisostearate (CAS number 68958-48-5).

In one embodiment, the mass ratio of the glycerin diisostearate to the oil phase component is 1: (4-5).

Preferably, the mass ratio of the glycerol diisostearate to the oil phase components is 1: 4.3.

in the research process, the inventor finds that emulsifiers such as glycerol stearate, glycerol isostearate, polyglycerol-10 diisostearate, polyglycerol-10 stearate, sorbitan isostearate, sorbitan laurate, PEG-20 glycerol triisostearate and the like can not produce probiotic-containing microecological particles with uniform particle sizes, and the inventor thinks that the emulsifiers are possibly poor in compatibility with oily components and thickening agents due to strong hydrophilicity, are not beneficial to forming round particles and are more difficult to form probiotic-containing microecological particles with uniform particle sizes. But the non-ionic surfactant with the HLB value of 2-5 is adopted, particularly the glycerol diisostearate is adopted, and the probiotics-containing micro-ecological particles prepared by the non-ionic surfactant are uniform, so that the reason considered by the inventor is probably that the glycerol diisostearate has stronger lipophilicity and lower HLB value, so that the glycerol diisostearate is favorable for forming emulsified particles which can wrap probiotics with oily components, the activity of the probiotics is improved, the colony structure of the skin is effectively improved, the balance of the skin micro-ecological flora is conditioned, and the skin barrier is improved to achieve long-acting moisture retention; in addition, the applicant found that when the mass ratio of the glycerin diisostearate to the oil phase components is 1: (4-5), the stability of the system can be obviously improved, and the reason that the inventor guesses is that the compatibility of the oil phase components of the diisostearate is good, the system is stable, and the formed probiotics-containing microecological particles are uniform.

In one embodiment, the probiotic micro-ecological particle containing component further comprises 0.01 to 0.05 parts by weight of a thickener.

Preferably, the component containing the probiotic micro-ecological particles further comprises 0.02 part by weight of a thickening agent.

Thickening agent

In one embodiment, the thickener is selected from one or more of carbomer, xanthan gum, hydroxyethyl cellulose, triethanolamine, sodium polyacrylate starch, sodium alginate, acrylic acid/C10-30 alkanol acrylate crosspolymer, locust bean gum.

Preferably, the thickening agent is a mixture of sodium alginate and acrylic acid/C10-30 alkanol acrylate cross-linked polymers.

In one embodiment, the mass ratio of sodium alginate to acrylic acid/C10-30 alkanol acrylate crosspolymer is (4-7): 1.

preferably, the mass ratio of the sodium alginate to the acrylic acid (ester)/C10-30 alkanol acrylate cross-linked polymer is 5: 1.

in the invention, the sodium alginate is a byproduct after iodine and mannitol are extracted from kelp or gulfweed of brown algae, the molecules of the sodium alginate are connected by beta-D-mannuronic acid (beta-D-mannuronic, M) and alpha-L-guluronic acid (alpha-L-guluronic acid, G) according to a (1 → 4) bond, and the sodium alginate is a natural polysaccharide. Sodium alginate is slightly soluble in water and insoluble in most organic solvents. It is dissolved in an alkaline solution to make the solution viscous. The sodium alginate powder becomes wet in water, and the surface of the sodium alginate powder has viscosity due to the hydration of the particles. The particles then quickly bind together to form agglomerates, which slowly hydrate and dissolve completely. Sodium alginate is more difficult to dissolve in water if it contains other compounds that compete with alginate for hydration. Sugar, starch or protein in the water will reduce the hydration rate of sodium alginate and longer mixing times are necessary.

In one embodiment, the sodium alginate is available under the brand name KIMICA ALGIN I-8-150, available from Kimica corporation, Japan.

In the present invention, the acrylic/C10-30 Alkyl Acrylate crosslinked Polymer (acrylics C10-30Alkyl Acrylate polymers) is a film forming agent (A film forming agent) which can be used as a thickener (thickner) in cosmetics, mainly in transparent gels and liquids, and in some sunscreen and facial scrub products, and the formed film can provide a water locking function for the product without adverse reaction or irritation.

In one embodiment, the acrylic/C10-30 alkanol acrylate crosspolymer is available under the trademark Pemulen TR-2Polymeric Emulsifier, available from Loborun oil group, Inc., USA.

(1) heating deionized water;

(3) mixing the rest oily components except silicone oil with surfactant, dissolving at 65-90 deg.C, adding probiotic composition, stirring, and transferring into water phase;

In the present invention, the oily components other than silicone oil include solid oily components and other liquid oily components, that is, the oily components other than silicone oil include solid oily components and remaining liquid oily components other than silicone oil.

In one embodiment, the preparation method of the probiotic-containing micro-ecological particle comprises the following steps:

(1) heating deionized water to 70-90 deg.C;

(2) adding a thickening agent into silicone oil, homogenizing and dispersing at a high speed, adding the mixture into a water phase, and stirring; the rotation speed of the homogeneous dispersion is 7000-9000 rpm; the stirring speed is 250-350 rpm;

(3) mixing the rest oily components except silicone oil with surfactant, dissolving at 65-90 deg.C, adding probiotic composition, stirring, transferring into water phase, and controlling particle size by rotation speed;

(4) cooling to 30-50 ℃, stopping stirring, and standing for 6-24h to obtain the probiotic-containing micro-ecological particles.

In a preferred embodiment, the preparation method of the probiotic-containing micro-ecological particle comprises the following steps:

(1) heating deionized water to 80 ℃;

(2) adding the thickening agent into silicone oil, homogenizing and dispersing, adding the mixture into a water phase, and stirring; the rotation speed of the homogeneous dispersion is 8000 rpm; the stirring rate was 320 rpm;

(3) mixing the rest oily components except the silicone oil and the surfactant, dissolving at 80 ℃, adding the probiotic composition, stirring, immediately transferring into a water phase, stirring, and controlling the stirring speed to be 310 rpm;

(4) and (3) cooling to 40 ℃, stopping stirring, and standing for 12h to obtain the probiotic-containing micro-ecological particles.

The inventor finds in the research that the addition of the solid oil and the liquid oil together can only form an emulsion, and it is difficult to form round particles. But the solid oil component and the liquid oil component are added separately, namely, the thickening agent is added into the liquid oil component, the thickening agent and the surfactant are added into the water phase after being uniformly dispersed, the solid oil component is stirred, the probiotic composition is added, the mixture is stirred and transferred into the water phase, the stirring speed is controlled to be 300-310rpm, the activity of the probiotics coated by the emulsified particles can be improved, particles with uniform particle size can be formed, the average particle size is 0.1-1mm, the system stability is good, and the phenomena of particle deformation or trace oil discharge and the like can not occur after the probiotics-containing micro-ecological particles are placed under the conditions of normal temperature, 45 ℃, minus 10 ℃ and light irradiation for 1 week, 2 weeks and 1 month. The reason the inventor guesses may be that the lipophilicity of the system is improved by the liquid oil and the glycerol diisostearate, and the thickening agent is added into the liquid oil to facilitate the formation of probiotic-containing microecological particles; the behenyl alcohol and the batyl alcohol can form a natural protective film to improve the affinity of probiotics, the surface tension of polydimethylsiloxane, squalane, pentaerythritol tetraisostearate and the like is small, the oil compatibility is good, the stability of probiotics-containing micro-ecological particles is improved under the action of sodium alginate and acrylic acid (ester)/C10-30 alkanol acrylate cross-linked polymer, and the particles do not deform or slightly produce oil after being stored for 1 week, 2 weeks and 1 month at normal temperature and 45 ℃ to 10 ℃ under the illumination condition.

In one embodiment, the name of the cosmetic product to which the probiotic-containing micro-ecological particles are added may be listed as: a gelan-Francisella-Dolichi ocean energy water supplement liquid, a gelan-Francisella-Dolichi ocean energy water supplement essence, a HOLA-Freund-whitening skin base liquid, and a HOLA-milk-popping-up-water.

In one embodiment, the probiotic-containing micro-ecological particles are added in an amount of 1-10 wt% based on the mass of the cosmetic.

The present invention will be specifically described below by way of examples. It should be noted that the following examples are only for illustrating the present invention and should not be construed as limiting the scope of the present invention, and that the insubstantial modifications and adaptations of the present invention by those skilled in the art based on the above disclosure are still within the scope of the present invention.

In addition, the starting materials used are all commercially available, unless otherwise specified.

Examples

Example 1

Embodiment 1 provides a probiotic-containing micro-ecological particle, which comprises the following components in parts by weight: 60 parts of deionized water, 5 parts of oily components, 0.2 part of probiotic composition, 1 part of surfactant and 0.01 part of thickening agent;

the oily component is a mixture of solid oil and liquid oil; the mass ratio of the solid oil component to the liquid oil component is 1: 2;

the solid oil component is a mixture of behenyl alcohol and batyl alcohol; the mass ratio of the behenyl alcohol to the batyl alcohol is 2.5: 1; the CAS of the behenyl alcohol is 661-19-8; the CAS number of the batyl alcohol is 544-62-7;

the liquid oil is a mixture of polydimethylsiloxane, squalane and pentaerythritol tetraisostearate; the mass ratio of the polydimethylsiloxane to the squalane to the pentaerythritol tetraisostearate is 0.5: 1: 1.1; the grade of the polydimethylsiloxane is KF-96A-6T, and the polydimethylsiloxane is purchased from the Japan shin-Etsu group; the CAS number of the squalane is 111-01-3; the CAS number of the pentaerythritol tetraisostearate is 62125-22-8;

the probiotic composition is a mixture of La Flora EC-12, Repair complete CLR PF and FermentageChardonnaY BG; the mass ratio of the La Flora EC-12, the Repair complete CLR PF and the FermentageChardonNAY BG is 1: 8: 4; the La Flora EC-12 is a lactic acid bacteria fermentation product, purchased from Nippon mono-pill company; the Repair Complex CLR PF is a split yeast fermentation product lysate purchased from CLR, Inc.; the nutrient Chardonnay BG comprises fermented grape extract, glutathione and lactobacillus, and is purchased from Nippon mono-pill company;

the surfactant is glycerol diisostearate (CAS number is 68958-48-5);

the thickening agent is a mixture of sodium alginate and acrylic acid (ester)/C10-30 alkanol acrylate cross-linked polymer; the mass ratio of the sodium alginate to the acrylic acid (ester)/C10-30 alkanol acrylate cross-linked polymer is 1: 4; the sodium alginate is KIMICA ALGIN I-8-150, and is purchased from Kimica corporation of Japan; the acrylic/C10-30 alkanol acrylate crosspolymer was sold under the trademark Pemulen TR-2Polymeric Emulsifier and was purchased from Luborun oil group, Inc., USA.

The preparation method of the probiotic-containing microecological particle comprises the following steps:

(1) heating deionized water to 70 ℃;

(2) adding a thickening agent into polydimethylsiloxane, homogenizing and dispersing, adding the mixture into a water phase, and stirring; the rotation speed of the homogeneous dispersion is 7000 rpm; the stirring rate was 250 rpm;

(3) mixing the rest oily components except for the polydimethylsiloxane and the surfactant, dissolving at 80 ℃, adding the probiotic composition, stirring uniformly, immediately transferring into a water phase, stirring, and controlling the stirring speed to be 250 rpm;

(4) cooling to 30 ℃, stopping stirring, and standing for 6h to obtain the probiotic-containing micro-ecological particles.

Example 2

Embodiment 2 provides a probiotic-containing micro-ecological particle, which comprises the following components in parts by weight: 90 parts of deionized water, 20 parts of oily components, 3 parts of probiotic composition, 5 parts of surfactant and 0.05 part of thickening agent;

the oily component is a mixture of solid oil and liquid oil; the mass ratio of the solid oil component to the liquid oil component is 1: 4;

the solid oil component is a mixture of behenyl alcohol and batyl alcohol; the mass ratio of the behenyl alcohol to the batyl alcohol is 3.5: 1; the CAS of the behenyl alcohol is 661-19-8; the CAS number of the batyl alcohol is 544-62-7;

the liquid oil is a mixture of polydimethylsiloxane, squalane and pentaerythritol tetraisostearate; the mass ratio of the polydimethylsiloxane to the squalane to the pentaerythritol tetraisostearate is (0.9: 1: 1.5; the trademark of the polydimethylsiloxane is KF-96A-6T and is purchased from Japan shin-Etsu group; the CAS number of the squalane is 111-01-3; and the CAS number of the pentaerythritol tetraisostearate is 62125-22-8;

the probiotic composition is a mixture of La Flora EC-12, Repair complete CLR PF and FermentageChardonnaY BG; the mass ratio of the La Flora EC-12, the Repair complete CLR PF and the FermentageChardonNAY BG is 1: 12: 8; the La Flora EC-12 is a lactic acid bacteria fermentation product, purchased from Nippon mono-pill company; the Repair Complex CLR PF is a split yeast fermentation product lysate purchased from CLR, Inc.; the nutrient Chardonnay BG comprises fermented grape extract, glutathione and lactobacillus, and is purchased from Nippon mono-pill company;

the surfactant is glycerol diisostearate (CAS number is 68958-48-5);

the thickening agent is a mixture of sodium alginate and acrylic acid (ester)/C10-30 alkanol acrylate cross-linked polymer; the mass ratio of the sodium alginate to the acrylic acid (ester)/C10-30 alkanol acrylate cross-linked polymer is 1: 7; the sodium alginate is KIMICA ALGIN I-8-150, and is purchased from Kimica corporation of Japan; the acrylic/C10-30 alkanol acrylate crosspolymer was sold under the trademark Pemulen TR-2Polymeric Emulsifier and was purchased from Luborun oil group, Inc., USA.

(1) heating deionized water to 90 ℃;

(2) adding a thickening agent into polydimethylsiloxane, homogenizing and dispersing, adding the mixture into a water phase, and stirring; the rotation speed of the homogeneous dispersion is 9000 rpm; the stirring rate was 350 rpm;

(3) mixing the rest oily components except for the polydimethylsiloxane and the surfactant, dissolving at 80 ℃, adding the probiotic composition, stirring uniformly, immediately transferring into a water phase, stirring, and controlling the stirring speed to be 350 rpm;

(4) and (3) cooling to 50 ℃, stopping stirring, and standing for 24 hours to obtain the probiotic-containing micro-ecological particles.

Example 3

Embodiment 3 provides a probiotic-containing micro-ecological particle, which comprises the following components in parts by weight: 75 parts of deionized water, 13 parts of oily components, 1.6 parts of probiotic composition, 3 parts of surfactant and 0.02 part of thickening agent;

the oily component is a mixture of solid oil and liquid oil; the mass ratio of the solid oil component to the liquid oil component is 1: 3;

the solid oil component is a mixture of behenyl alcohol and batyl alcohol; the mass ratio of the behenyl alcohol to the batyl alcohol is 3.1: 1; the CAS of the behenyl alcohol is 661-19-8; the CAS number of the batyl alcohol is 544-62-7;

the liquid oil is a mixture of polydimethylsiloxane, squalane and pentaerythritol tetraisostearate; the mass ratio of the polydimethylsiloxane to the squalane to the pentaerythritol tetraisostearate is 0.7: 1: 1.3; the grade of the polydimethylsiloxane is KF-96A-6T, and the polydimethylsiloxane is purchased from the Japan shin-Etsu group; the CAS number of the squalane is 111-01-3; the CAS number of the pentaerythritol tetraisostearate is 62125-22-8;

the probiotic composition is a mixture of La Flora EC-12, Repair complete CLR PF and FermentageChardonnaY BG; the mass ratio of the La Flora EC-12, the Repair complete CLR PF and the FermentageChardonNAY BG is 1: 10: 6; the La Flora EC-12 is a lactic acid bacteria fermentation product, purchased from Nippon mono-pill company; the Repair Complex CLR PF is a split yeast fermentation product lysate purchased from CLR, Inc.; the nutrient Chardonnay BG comprises fermented grape extract, glutathione and lactobacillus, and is purchased from Nippon mono-pill company;

the surfactant is glycerol diisostearate (CAS number is 68958-48-5);

the thickening agent is a mixture of sodium alginate and acrylic acid (ester)/C10-30 alkanol acrylate cross-linked polymer; the mass ratio of the sodium alginate to the acrylic acid (ester)/C10-30 alkanol acrylate cross-linked polymer is 1: 5; the sodium alginate is KIMICA ALGIN I-8-150, and is purchased from Kimica corporation of Japan; the acrylic/C10-30 alkanol acrylate crosspolymer was sold under the trademark Pemulen TR-2Polymeric Emulsifier and was purchased from Luborun oil group, Inc., USA.

(1) heating deionized water to 80 ℃;

(2) adding a thickening agent into polydimethylsiloxane, homogenizing and dispersing, adding the mixture into a water phase, and stirring; the rotation speed of the homogeneous dispersion is 8000 rpm; the stirring rate was 320 rpm;

(3) mixing the rest oily components except for the polydimethylsiloxane and the surfactant, dissolving at 80 ℃, adding the probiotic composition, stirring uniformly, immediately transferring into a water phase, stirring, and controlling the stirring speed to be 310 rpm;

Example 4

Example 4 provides a probiotic-containing probiotic micro-ecological particle, the only difference being that the surfactant is glyceryl isostearate (CAS No. 61332-02-3) as in example 3.

The preparation method of the probiotic-containing micro-ecological particles is the same as that of example 3.

Example 5

Example 5 provides a probiotic bacteria-containing probiotic bacteria-.

Example 6

Example 6 provides a probiotic-containing probiotic micro-ecological particle, the same as example 3, except that the surfactant is polyglycerol-10 diisostearate (CAS No. 63705-03-3).

Example 7

Example 7 provides a probiotic-containing probiotic micro-ecological particle, the same as example 3, except that the surfactant is polyglycerol-10 stearate (CAS No. 72347-89-8).

Example 8

Example 8 provides a probiotic bacteria-containing micro-ecological particle, the same as example 3, except that no surfactant is present.

(1) heating deionized water to 80 ℃;

Example 9

Example 9 provides a probiotic-containing micro-ecological particle, the only difference being that the thickening agent is sodium alginate, as in example 3.

Example 10

Example 10 provides a probiotic bacteria-containing micro-ecological particle as in example 3.

(1) heating deionized water to 80 ℃;

(2) adding a thickening agent into polydimethylsiloxane, homogenizing and dispersing, adding the mixture into a water phase, and stirring; the rotation speed of the homogeneous dispersion is 8000 rpm; the stirring rate was 200 rpm;

(3) mixing the rest oily components except for the polydimethylsiloxane and the surfactant, dissolving at 80 ℃, adding the probiotic composition, stirring uniformly, immediately transferring into a water phase, stirring, and controlling the stirring speed to be 200 rpm;

Example 11

Example 11 provides a probiotic bacteria-containing micro-ecological particle as in example 3.

(1) heating deionized water to 120 ℃;

(3) mixing the rest oily components except for the polydimethylsiloxane and the surfactant, dissolving at 80 ℃, adding the probiotic composition, stirring, immediately transferring into a water phase, stirring, and controlling the stirring speed to be 310 rpm;

Example 12

Example 12 provides a probiotic bacteria-containing micro-ecological particle as in example 3.

(1) heating deionized water to 80 ℃;

(3 mixing the rest oily components except the polydimethylsiloxane with a surfactant, dissolving at 80 ℃, adding the probiotic composition, stirring, immediately transferring into a water phase, and stirring at the speed of 310 rpm;

Example 13

Example 13 provides a probiotic bacteria-containing micro-ecological particle as in example 3.

(1) heating deionized water to 80 deg.C, adding thickener, oily component (mixing solid oil component, polydimethylsiloxane, and other liquid oil components except polydimethylsiloxane), probiotic composition, and surfactant, and homogenizing at 8000 rpm;

(2) then stirring; the stirring rate was 320 rpm;

(3) and (3) cooling to 40 ℃, stopping stirring, and standing for 12h to obtain the probiotic-containing micro-ecological particles.

Performance testing

1. Storage stability performance: the probiotic-containing micro-ecological particles described in examples 1 to 13 were stored at room temperature, 45 ℃ and-10 ℃ under light for 1 week, 2 weeks and 1 month, and the appearance of the particles was visually compared with the samples prepared immediately before, and the stability was evaluated according to the following criteria:

a: no significant change in the sample was observed under this condition;

b: deformation or slight oil production is confirmed when less than 15% of the particles exist;

c: more than 15% of the particles are confirmed to be deformed or obviously oiled;

the test results are shown in tables 1 to 4, in which table 1 shows the storage stability at room temperature, table 2 shows the storage stability at 45 ℃, table 3 shows the storage stability at-10 ℃, and table 4 shows the storage stability under light conditions;

TABLE 1

TABLE 2

TABLE 3

TABLE 4

2. Average particle size: the probiotic-containing micro-ecological particles described in examples 1 to 13 were observed on a biological microscope, and particle size data were recorded, wherein an average particle size of 0.1 to 1mm was denoted as a, an average particle size of less than 0.1mm was denoted as B, an average particle size of more than 1mm was denoted as C, and an unformed particle was denoted as D, and the test results are shown in table 5.

TABLE 5

3. Testing the moisturizing effect: adding the probiotic-containing micro-ecological particles of examples 1-3 to a gram-free fran-polyli ocean energy supplement solution (the probiotic-containing micro-ecological particles of examples 1-3 are added in an amount of 2 wt%); the test method comprises the following steps: selecting 50 subjects of 25-35 years of age, and randomly dividing the subjects into 5 groups of 10 persons; the specific method comprises the following steps:

after cleansing the face, the subjects rested in a constant environment for 3 minutes, and then tested for stratum corneum moisture content using a stratum corneum moisture content tester (Corneometer CM825, Courage & Khazaka, germany). The average value is taken by 3 times of continuous tests, and the higher the parameter value, the higher the water content of the stratum corneum of the skin is. Then uniformly smearing a test sample by using a latex glove, and starting timing; the change of the measurement values with respect to time according to the experimental design is shown in Table 6.

TABLE 6

4. Human skin testing

Adding the probiotic-containing microecological particles described in example 3 into a Glan Mayland Dolisi ocean energy moisturizing essence (the probiotic-containing microecological particles described in example 3 are added in an amount of 2 wt%) and applying the essence to the surface of human skin; after 5 female subjects with the ages of 25-40 years are selected and skin conditions are recorded, the essence prepared in the embodiment is continuously used for one week, the skin condition of each female subject is observed and compared with the previous skin condition, and the experimental results of some female subjects are as follows:

1. the product is used for one week after trial, the skin is obviously and evenly moistened, the skin color is brightened, and the phenomenon of allergy does not occur.

2. The acne mark on the skin is obvious in 31 years old, the acne mark still has the phenomenon of acne growing at present, after the product is tried for one week, the acne mark becomes obviously light, the skin is gradually improved, leveled and smooth, and the phenomenon of acne growing is relieved.

3. The product is used for one week after the product is tried, the fine lines are obviously reduced, and the skin is moist, bright and free from tightness.

4. The product is oily in the T area and dry in the cheek and has sensitive skin phenomenon in 30 years old, and after the product is continuously used for one week, the T area and the cheek phenomenon are improved, the skin is not tight, and the skin has certain glossiness and is not allergic.

5. The skin care product is 26 years old, has mixed skin and thinner cuticle, and has a certain amount of pox on the face which are all obviously red, after the product is tried for one week, the pox and red phenomena are all relieved, and the skin is moist without allergic phenomena.

The foregoing examples are merely illustrative and serve to explain some of the features of the method of the present invention. The appended claims are intended to claim as broad a scope as is contemplated, and the examples presented herein are merely illustrative of selected implementations in accordance with all possible combinations of examples. Accordingly, it is applicants' intention that the appended claims are not to be limited by the choice of examples illustrating features of the invention. Also, where numerical ranges are used in the claims, subranges therein are included, and variations in these ranges are also to be construed as possible being covered by the appended claims.

Claims

1. The probiotic-containing microecological particle is characterized by comprising the following components in parts by weight: 60-90 parts of deionized water, 5-20 parts of oily components, 0.2-3 parts of probiotic composition and 1-5 parts of surfactant; the oily component comprises solid oil component and liquid oil component; the mass ratio of the solid oil component to the liquid oil component is 1: (2-4).

2. The probiotic-containing micro-ecological particle according to claim 1, characterized in that the average particle size of the probiotic-containing micro-ecological particle is 0.1-1 mm.

3. The probiotic-containing micro-ecological particle according to claim 1, wherein the probiotic composition is selected from one or more of La Flora EC-12, Repair Complex CLR PF, and nutrient CHARDONNAY BG.

4. Probiotic-containing micro-ecological particles according to any of claims 1-3, characterized in that said liquid oil content comprises silicone oil and other liquid oil content.

5. Probiotic bacteria-containing micro-ecological particles according to claim 4, characterized in that the other liquid oil components are selected from one or more of olive oil, sweet almond oil, grape seed oil, glycerol tri (ethylhexanoate), squalane, pentaerythritol tetraisostearate, pentaerythritol tetra (ethylhexanoate), caprylic/capric triglyceride, isononyl isononanoate, cetyl ethylhexanoate.

6. Probiotic bacteria-containing micro-ecological particle according to any of claims 1 to 3, characterized in that the surfactant has an HLB value of 2 to 5.

7. The probiotic-containing micro-ecological particle according to claim 4, characterized in that the composition of the probiotic-containing micro-ecological particle further comprises 0.01-0.05 parts by weight of a thickening agent.

8. The probiotic-containing micro-ecological particle according to claim 7, wherein the thickener is selected from one or more of carbomer, xanthan gum, hydroxyethyl cellulose, triethanolamine, sodium polyacrylate starch, sodium alginate, acrylic acid/C10-30 alkanol acrylate crosspolymer, locust bean gum.

9. A method for preparing probiotic bacteria-containing micro-ecological particles according to claim 7 or 8, characterized in that it comprises at least the following steps:

(1) heating deionized water;

10. Use of probiotic bacteria-containing micro-ecological particles in a cosmetic according to any one of claims 1 to 8, wherein the cosmetic is selected from one or more of a cream, a mask, a serum, a toner, a lotion, a face milk, a moisturizing liquid, a skin lotion.