CN111728915A - Membrane patch for improving neck wrinkles and preparation method thereof - Google Patents

Membrane patch for improving neck wrinkles and preparation method thereof Download PDF

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CN111728915A
CN111728915A CN202010797664.0A CN202010797664A CN111728915A CN 111728915 A CN111728915 A CN 111728915A CN 202010797664 A CN202010797664 A CN 202010797664A CN 111728915 A CN111728915 A CN 111728915A
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parts
purified water
phase
stirring
patch
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李剑平
马关保
刘悦
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Heilongjiang Pharmaceutical Co ltd
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Heilongjiang Pharmaceutical Co ltd
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    • A61K8/96Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
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    • A61K8/02Cosmetics or similar toiletry preparations characterised by special physical form
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    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
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    • A61K8/00Cosmetics or similar toiletry preparations
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    • A61K8/8129Compositions of homopolymers or copolymers of compounds having one or more unsaturated aliphatic radicals, each having only one carbon-to-carbon double bond, and at least one being terminated by an alcohol, ether, aldehydo, ketonic, acetal or ketal radical; Compositions of hydrolysed polymers or esters of unsaturated alcohols with saturated carboxylic acids; Compositions of derivatives of such polymers, e.g. polyvinylmethylether
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    • AHUMAN NECESSITIES
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Abstract

A membrane patch for improving neck wrinkles and a preparation method thereof belong to the technical field of biology. It has solved the poor problem of continuous effect of moisturizing that current neck membrane subsides exist. Film pasting: is prepared from polyvinyl alcohol 1788, folium kaki powder, sodium hyaluronate, glycerol, titanium dioxide, polyvinylpyrrolidone, sodium carboxymethylcellulose, carbomer dry powder, orchid essence and purified water. The method comprises the following steps: weighing raw materials; preparing phase A from carbomer dry powder and purified water; preparing a B phase from polyvinyl alcohol 1788, purified water, polyvinylpyrrolidone and sodium carboxymethyl cellulose; adding phase B into phase A, stirring to obtain persimmon leaf powder and sodium hyaluronate, and stirring to obtain phase C; mixing titanium dioxide, glycerol, orchid essence and purified water, adding into phase C, and stirring. The film patch for improving the neck wrinkles, prepared by the invention, can realize film forming, ventilation, long-acting moisture preservation, wrinkle removal, whitening, neck line reduction, skin microcirculation improvement and cell metabolism acceleration. The invention is suitable for improving neck wrinkles.

Description

Membrane patch for improving neck wrinkles and preparation method thereof
Technical Field
The invention belongs to the technical field of biology, and particularly relates to a membrane patch for improving neck wrinkles and a preparation method thereof.
Background
Skin aging is divided into chronological aging and photoaging. Chronological aging, also known as natural aging, is caused by immaterial factors existing in the body, such as heredity, gravity, endocrine and immune functions, etc., with the increase of age. Wrinkles are the manifestation of aging of the skin of the body, and the appearance of wrinkles is regulated by a predetermined genetic program of the body. Three classes of expressed genes associated with skin aging have been detected: the proto-oncogenes C-myc and C-fos; a gene encoding a receptor (EGFR); the cloned gene (GADD 153). The results of the study showed that the degree of wrinkles worsened with age at the same site. The reason for forming neck wrinkles is complex, and the congenital formation, photoaging, obesity, dryness, water deficiency, gravity action, repeated head-lowering and squeezing and the like can cause the neck skin to be loose, the dermis fiber to be broken, the neck transverse lines to be formed, the lower jaw edge to be fuzzy and the like. The amount of neck wrinkles is used as one of the aging criteria; although there are skin care products, laser, botulinum toxin injection, and other preventive or therapeutic methods, the effects are limited. In clinical treatment, past practices show that the treatment of neck wrinkles is still a relatively troublesome problem, and no exact method can completely solve the problem.
The skin is divided into epidermis, dermis, subcutaneous tissue and skin appendages. The epidermis is located at the outermost layer of the skin and is divided into a basal layer, a spinous layer, a granular layer, a transparent layer and a horny layer from inside to outside. The outermost horny layer can effectively prevent external harmful factors from invading human body and prevent the loss of water and nutrient substances in the human body. The skin barrier is likened by the scholars to the "wall theory", epidermal keratinocytes are layered one on top of the other, the epidermal cells are "brick (brick)", and the intercellular spaces are "mortar (mortar)", which are tightly and seamlessly bonded and play a good skin barrier role. The water content of the healthy stratum corneum should be kept between 10% and 20%, and less than 10% of the skin will be dry, rough, wrinkled and even chapped. The water content in the stratum corneum thus determines the physical state and appearance of the skin, such as the elasticity and plumpness of the skin. In order to maintain the skin in a good condition, a certain amount of water is contained in the horny layer in addition to the oleaginous substance having a moisturizing effect. Maintaining skin moisturization is important in reducing wrinkle formation.
The invention provides a novel sleep neck mask, which aims at solving the problem that the existing mask is poor in moisturizing lasting effect.
Disclosure of Invention
The invention aims to solve the problem that the existing neck mask is poor in moisturizing lasting effect, and provides a mask for improving neck wrinkles and a preparation method thereof.
A film patch for improving neck wrinkles is prepared from, by weight, 4-6 parts of polyvinyl alcohol 1788, 4-6 parts of persimmon leaf powder, 0.6-1.2 parts of sodium hyaluronate, 4-6 parts of glycerol, 0.1-0.6 part of titanium dioxide, 1-3 parts of polyvinylpyrrolidone, 1-2 parts of sodium carboxymethylcellulose, 0.5-2 parts of carbomer dry powder, 0.05-0.2 part of orchid essence and 30-40 parts of purified water.
The preparation method of the membrane patch for improving the neck wrinkles is realized according to the following steps:
weighing 4-6 parts of polyvinyl alcohol 1788, 4-6 parts of persimmon leaf powder, 0.6-1.2 parts of sodium hyaluronate, 4-6 parts of glycerol, 0.1-0.6 part of titanium dioxide, 1-3 parts of polyvinylpyrrolidone, 1-2 parts of sodium carboxymethylcellulose, 0.5-2 parts of carbomer dry powder, 0.05-0.2 part of orchid essence and 30-40 parts of purified water as raw materials in parts by weight;
secondly, dispersing the weighed carbomer dry powder into 10-15 parts of purified water, and standing for 18-24 hours to obtain a phase A;
thirdly, adding the weighed polyvinyl alcohol 1788 into 10-15 parts of purified water, ultrasonically dissolving for 20-40 min, then adding polyvinylpyrrolidone and sodium carboxymethylcellulose, and stirring at the rotating speed of 7000-9000 r/min for 20-40 min to obtain a phase B;
fourthly, adding the phase B into the phase A, mixing and stirring for 20min, adding the weighed persimmon leaf powder and sodium hyaluronate, mixing and stirring for 20-40 min, and obtaining a phase C;
and fifthly, adding the weighed titanium dioxide, glycerol and orchid essence into the rest purified water, mixing and stirring for 20-40 min, then adding into the phase C, and stirring for 20-40 min, thus finishing the preparation of the membrane patch for improving neck wrinkles.
According to the membrane patch for improving neck wrinkles, the raw materials of the membrane patch comprise polyvinylpyrrolidone, sodium carboxymethylcellulose, carbomer and other membrane forming emulsifiers, so that a molecular breathable membrane can be formed within 15min, and due to the fact that the humectant glycerin and the molecular emulsifying colloidal substance polyvinyl alcohol 1788 show a long-acting moisturizing effect in the membrane body, and the titanium dioxide, the persimmon leaf powder and the sodium hyaluronate are added, the membrane patch achieves the effects of removing wrinkles and whitening the neck, reducing neck wrinkles and accelerating cell metabolism.
The film patch for improving the neck wrinkles, prepared by the invention, can realize film forming, ventilation, long-acting moisture preservation, wrinkle removal, whitening, neck line reduction, skin microcirculation improvement and cell metabolism acceleration.
The invention is suitable for improving neck wrinkles.
Drawings
FIG. 1 is a histogram of the change trend of TEWL values in the examples;
FIG. 2 is a histogram of the variation trend of CAP values in the example;
FIG. 3 is a graph showing the change trend of the wrinkle area map in the examples, wherein ● represents the experimental group, and ■ represents the blank control group; tangle-solidup indicates negative control group;
FIG. 4 is a graph showing the variation of wrinkle length in examples, wherein ● represents the experimental group and ■ represents the blank control group; tangle-solidup indicates negative control group;
FIG. 5 is a graph showing the variation of wrinkle depth in examples, wherein ● represents an experimental group and ■ represents a blank control group; tangle-solidup indicates negative control group;
FIG. 6 is a graph showing the measurement trend of OD values in examples, wherein ● represents an experimental group and ■ represents a blank control group; tangle-solidup indicates negative control group.
Detailed Description
The technical solution of the present invention is not limited to the following specific embodiments, but includes any combination of the specific embodiments.
The first embodiment is as follows: the film patch for improving neck wrinkles is prepared from, by weight, 4-6 parts of polyvinyl alcohol 1788, 4-6 parts of persimmon leaf powder, 0.6-1.2 parts of sodium hyaluronate, 4-6 parts of glycerol, 0.1-0.6 part of titanium dioxide, 1-3 parts of polyvinylpyrrolidone, 1-2 parts of sodium carboxymethylcellulose, 0.5-2 parts of carbomer dry powder, 0.05-0.2 part of orchid essence and 30-40 parts of purified water.
The second embodiment is as follows: the difference between the embodiment and the specific embodiment is that the water-based paint is prepared from, by weight, 5 parts of polyvinyl alcohol 1788, 5 parts of persimmon leaf powder, 1 part of sodium hyaluronate, 5 parts of glycerol, 0.3 part of titanium dioxide, 1.75 parts of polyvinylpyrrolidone, 1.25 parts of sodium carboxymethylcellulose, 1 part of carbomer dry powder, 0.1 part of orchid essence and 25 parts of purified water. The rest is the same as the first embodiment.
The third concrete implementation mode: the difference between the embodiment and the specific embodiment is that the water-based paint is prepared from 4 parts by weight of polyvinyl alcohol 1788, 4 parts by weight of persimmon leaf powder, 0.6 part by weight of sodium hyaluronate, 4 parts by weight of glycerol, 0.1 part by weight of titanium dioxide, 1 part by weight of polyvinylpyrrolidone, 1 part by weight of sodium carboxymethylcellulose, 0.5 part by weight of carbomer dry powder, 0.05 part by weight of orchid essence and 30 parts by weight of purified water. The rest is the same as the first embodiment.
The fourth concrete implementation mode: the difference between the embodiment and the specific embodiment is that the water-based paint is prepared from 6 parts by weight of polyvinyl alcohol 1788, 6 parts by weight of persimmon leaf powder, 1.2 parts by weight of sodium hyaluronate, 6 parts by weight of glycerol, 0.6 part by weight of titanium dioxide, 3 parts by weight of polyvinylpyrrolidone, 2 parts by weight of sodium carboxymethylcellulose, 2 parts by weight of carbomer dry powder, 0.2 part by weight of orchid essence and 40 parts by weight of purified water. The rest is the same as the first embodiment.
The fifth concrete implementation mode: the preparation method of the membrane patch for improving the neck wrinkles in the embodiment comprises the following steps:
weighing 4-6 parts of polyvinyl alcohol 1788, 4-6 parts of persimmon leaf powder, 0.6-1.2 parts of sodium hyaluronate, 4-6 parts of glycerol, 0.1-0.6 part of titanium dioxide, 1-3 parts of polyvinylpyrrolidone, 1-2 parts of sodium carboxymethylcellulose, 0.5-2 parts of carbomer dry powder, 0.05-0.2 part of orchid essence and 30-40 parts of purified water as raw materials in parts by weight;
secondly, dispersing the weighed carbomer dry powder into 10-15 parts of purified water, and standing for 18-24 hours to obtain a phase A;
thirdly, adding the weighed polyvinyl alcohol 1788 into 10-15 parts of purified water, ultrasonically dissolving for 20-40 min, then adding polyvinylpyrrolidone and sodium carboxymethylcellulose, and stirring at the rotating speed of 7000-9000 r/min for 20-40 min to obtain a phase B;
fourthly, adding the phase B into the phase A, mixing and stirring for 20min, adding the weighed persimmon leaf powder and sodium hyaluronate, mixing and stirring for 20-40 min, and obtaining a phase C;
and fifthly, adding the weighed titanium dioxide, glycerol and orchid essence into the rest purified water, mixing and stirring for 20-40 min, then adding into the phase C, and stirring for 20-40 min, thus finishing the preparation of the membrane patch for improving neck wrinkles.
The second step of the embodiment is placed for 18-24 hours to fully swell the carbomer dry powder and avoid agglomeration.
The sixth specific implementation mode: the difference between the embodiment and the fifth embodiment is that in the first step, 5 parts of polyvinyl alcohol 1788, 5 parts of persimmon leaf powder, 0.8 part of sodium hyaluronate, 5 parts of glycerin, 0.3 part of titanium dioxide, 2 parts of polyvinylpyrrolidone, 1.5 parts of sodium carboxymethylcellulose, 1.5 parts of carbomer dry powder, 0.1 part of orchid essence and 35 parts of purified water are weighed as raw materials. The other steps and parameters are the same as those in the fifth embodiment.
The seventh embodiment: the difference between this embodiment and the fifth or sixth embodiment is that in the second step, the mixture is dispersed in 15ml of purified water and left for 20 hours. The other steps and parameters are the same as those of the fifth or sixth embodiment.
The specific implementation mode is eight: the difference between the fifth embodiment and the seventh embodiment is that the third embodiment is added into 10 parts of purified water and dissolved by ultrasonic for 30 min. Other steps and parameters are the same as those of one of the fifth to seventh embodiments.
The specific implementation method nine: the difference between this embodiment and the fifth to eighth embodiment is that the stirring is carried out at 8000r/min for 30min in the third step. Other steps and parameters are the same as those of the fifth to eighth embodiments.
The detailed implementation mode is ten: the difference between this embodiment and one of the fifth to ninth embodiments is that the mixing and stirring are performed for 30min in the fourth step. Other steps and parameters are the same as those in one of the fifth to ninth embodiments.
The concrete implementation mode eleven: the difference between the fifth embodiment and the tenth embodiment is that in the fifth embodiment, the mixture is mixed and stirred for 30min, and then added into the phase C and stirred for 30 min. Other steps and parameters are the same as those in one of the fifth to tenth embodiments.
The beneficial effects of the present invention are demonstrated by the following examples:
example (b):
a preparation method of a film patch for improving neck wrinkles is realized according to the following steps:
firstly, dispersing 1g of carbomer dry powder in 15ml of purified water, and standing for 20 hours to obtain a phase A;
secondly, adding 5g of polyvinyl alcohol 1788 into 10ml of purified water, ultrasonically dissolving for 30min, then adding 1.75g of polyvinylpyrrolidone and 1.25g of sodium carboxymethylcellulose, and stirring for 30min at the rotating speed of 8000r/min to obtain a phase B;
adding the phase B into the phase A, mixing and stirring for 20min, adding 5g of persimmon leaf powder and 1g of sodium hyaluronate, mixing and stirring for 30min to obtain a phase C;
fourthly, adding 0.3g of titanium dioxide, 5g of glycerol and 0.1g of orchid essence into 10ml of purified water, mixing and stirring for 30min, then adding into the phase C and stirring for 30min, and finishing the preparation of the membrane patch for improving neck wrinkles.
In this example, the obtained film patch for improving neck wrinkles was prepared as a sample, and effect verification was performed:
1. subject screening: 10 healthy subjects aged 19-36 years, the average age was (26.70 +/-4.64), the subjects were mainly indoors, and no history of a large amount of sun exposure and no history of light-sensitive diseases; the measurement site is free of skin disorders, pigmentation, scarring or systemic diseases that may affect the skin.
2. The experimental principle is as follows:
1) the moisturizing effect is as follows:
(ii) transcutaneous water loss (TEWL): the TEWL shows the water loss condition by measuring the water vapor pressure gradient on the surface of the skin, thereby reflecting the water permeability barrier of the skin, the larger the TEWL value is, the poorer the skin barrier function is, the stronger the absorption capacity is, the more easily the skin is damaged by the outside harmful substances, the more the water is lost from the unit area in unit time, the drier and water-deficient the skin is.
② horny layer water Content (CAP): the moisture content of the stratum corneum is calculated by the moisture content measuring instrument according to the capacitance of the skin, the higher the moisture content of the stratum corneum is, the better the moisturizing effect of the product is, and the more light the skin has.
2) And (3) long-acting wrinkle removal: visinoline VL650 wrinkle analysis System photographs of a subject sample taken before use, with 5 days of sample continued use were analyzed to obtain total wrinkle area (mm)2) The average wrinkle length (mm) and average wrinkle depth (μm) indicate changes in wrinkles, which reflect the degree of aging.
3) Whitening effect: the change in skin color was measured using a Lab colorimetric system spectrophotometer (Meineng Lab Darby spectrophotometer CM-2002). The test index formula is as follows:
L=116(Y/Yn)1/3-16;
a=500[(X/Xn)1/3-(Y/Yn)1/3];
B=200[(Y/Yn)1/3-(Z/Zn)1/3];
Cab=(a2+b2)1/2
hab=arctg(b/a);
l, a and b are coordinate values of a three-dimensional rectangular coordinate system; x, Y, Z is the tristimulus value of the color system; xN、TN、ZNThe total diffuse reflection surface has three stimulus values, L is brightness, the color is more towards white when the value is larger, and is conversely more towards black, a is red and green, a is red direction, a is green direction, b is yellow and blue, b is yellow direction, b is blue direction, c is saturation for representing the shade of the surface color of the object, H is hue angle for representing the characteristic that the colors are mutually separated, in a chromaticity system, the color difference ▽ E between the two stimuli is represented by the difference ▽ L, ▽ a and ▽ b of coordinates L, a and bab,ΔEab=[(ΔL)2+(Δa)2+(Δb)2]1/2I.e. the distance between two color points in color space, where ▽ E is a factorabIs a comprehensive index of L, a and b, represents the three-dimensional change of chromaticity, and is a more appropriate index for evaluating the skin color change.
4) Metabolism speed:
skin metabolism mainly depends on the circulation speed of skin microcirculation, and the laser Doppler blood flow instrument and the capillary blood vessel microcirculation instrument are adopted in the experiment to test the microcirculation speed of the neck skin before and after the film is applied.
Detecting the proliferation capacity change before and after the skin is applied with the adhesive film by an MTT method; the main mechanism is that the deoxyenzyme in living cells can be reduced into water-insoluble blue products and precipitated in the cells, while dead cells do not have the function. Can dissolve blue crystals deposited in cells, the color depth of the solution is in linear relation with the contained quantity, and the quantity of living cells can be reflected by the photometric density value.
3. The experimental process comprises the following steps:
1) the moisturizing effect is as follows: after all subjects are cleaned and aired, TEWL and CAP values are detected through a percutaneous water loss measuring instrument and a horny layer water content measuring instrument, a room with the room temperature of 22 +/-2 ℃ and the humidity of 47 +/-7% is required to be detected in the environment, the subjects need to lie in the room for 25min in an inclined mode before use, a piece of skin with the size of 2cm multiplied by 2cm is selected from the equivalent position of the neck of each subject to be used as a test area and marked, the physiological indexes of the skin are measured through the percutaneous water loss measuring instrument and the horny layer water content measuring instrument before treatment and after treatment at 5d, 10d and 15d, and the change of the values before treatment and after treatment.
2) And (3) long-acting wrinkle removal: the subject lies down with the left side of the head facing up, the eyes are closed, and the positioning adhesive is stuck on the mark points. Pouring a certain amount of silica gel into a small plastic box, adding a catalyst coagulant, quickly and uniformly stirring, uniformly coating the silica gel on an area defined by a positioning rubber ring, removing the silica gel after 10min, pasting the silica gel on a positioning paper board special for a Visio-lineVL650 wrinkle analysis system, placing the paper board pasted with the silica gel on a platform of Visoline VL650, adjusting focal light, enabling the light to be vertical to wrinkle lines, and analyzing after photographing. Results are expressed in total, wrinkle area (mm)2) The average wrinkle length (mm) and the average wrinkle depth (μm) are shown, and the photograph taken at the first time is used as a positioning reference in the subsequent analysis. Wrinkle changes using samples from 0-15d consecutively. No plaster, clear water as a control and a film as an experimental group were stuck.
3) Whitening effect: after all subjects clean the skin and dry in the air, the adhesive film is applied on a flat surface for 30min, the movement is reduced as much as possible in the period, the adhesive film is removed after the time is up, the skin is cleaned for 2-3 times by clear water, the application is kept for 0-15d, and then the change of the skin color is measured by an instrument. No plaster, clear water as a control and a film as an experimental group were stuck.
4) Metabolism speed:
firstly, before the test is started, a subject enters a constant temperature room with the temperature of 25 ℃ to enable the body to adapt to the environmental temperature, and after the test is started, the subject lies on the bed in a supine position and is in a quiet state without any limb movement and severe conscious activities. Adjusting the probe of the laser Doppler blood flow instrument at the neck, placing and fixing the detection probe, recording the blood perfusion volume of the crinkles 5min after stabilization, and taking the value as a basic value. Recording every 5min for 1 time, 1min each time, taking average value, and observing for 15 min. No film was applied, a clear water film was applied as a control, and a neck film was applied as an experimental group.
Taking 15 rats with the weight of about 400g, removing surface hairs, applying a neck film every day at regular time, killing 3 rats every three days, and taking back skin cells for testing; we used the MTT method to test the cell proliferation ability. And (4) taking an unsticked film and a clean water film as a control group, and drawing a growth curve according to the cell number to reflect the cell proliferation capacity change. The results of the experiment were analyzed over 15 days of continuous comparison.
5) And (4) finishing data, and improving the wrinkle improvement effect of the neck wrinkle film paster through data analysis.
4. Analysis of results
The moisturizing effect is as follows: the skin physiological indexes before and after use are shown in table 1;
TABLE 1 skin physiological indices before and after use
TEWL CAP
Before use 22.2±7.2 32.2±6.4
5min after use 55.2±4.3 72.8±2.5
Day 5 after application 20.1±4.3 55.2±3.7
Day 6 after application 18.2±5.4 48.5±5.5
Day 7 after use 20.6±3.6 43.4±4.5
Trend of TEWL: referring to fig. 1, TEWL increased significantly after application of the cervical patch, and gradually decreased after application as measured at 5d, 6d, and 7d, and below before application.
Trend of CAP change: referring to fig. 2, CAP increased significantly after application of the cervical patch and decreased gradually after application at 5d, 6d, 7d, but higher than before application.
②, removing wrinkles for a long time: from the data in fig. 3 and 4, the total wrinkle area, wrinkle length; the experimental group (applied with the neck mask) is reduced compared with the negative control group (applied with the common clear water), the blank control group (not treated) has different wrinkle depths, as shown in fig. 5, the depth of the experimental group is not obviously reduced, the control group is increased and then decreased, and analysis may be influenced by experimental errors and the external environment received by the test subject in the later period.
③ whitening effect, ▽ E measured on different daysabAs shown in Table 2, it can be seen that ▽ E was observed in the experimental group (neck patch application) as compared with the control group (plain clear water application)abThe change in value was significant and the value was large, thus concluding that the patch could whiten the skin.
TABLE 2 measurements for different days ▽ EabValue of (A)
Figure BDA0002626257790000071
Figure BDA0002626257790000081
Fourthly, metabolism speed:
testing an instrument: see table 3, neck experiment blood perfusion volume measured value, it is obvious that experimental group blood perfusion speed improves, explains that metabolism speed is accelerated, and two contrast groups then change unobviously, explains that this pad pasting helps improving skin microcirculation and accelerates metabolism speed.
TABLE 3 measurement of perfusion volume (in ml/s) in cervical experiments
Figure BDA0002626257790000082
Measuring the OD value: as shown in fig. 6, the results showed that comparing the OD values of the experimental group (cervical patch) and the blank untreated group and the clear water patch showed that the proliferation potency of the cells of the experimental group was significantly enhanced and in a time-dependent relationship. The difference of the proliferation capacities of the two control groups is small, which indicates that the adhesive film promotes the in-vitro proliferation of skin cells.

Claims (10)

1. The membrane patch for improving neck wrinkles is characterized by being prepared from, by weight, 4-6 parts of polyvinyl alcohol 1788, 4-6 parts of persimmon leaf powder, 0.6-1.2 parts of sodium hyaluronate, 4-6 parts of glycerol, 0.1-0.6 part of titanium dioxide, 1-3 parts of polyvinylpyrrolidone, 1-2 parts of sodium carboxymethylcellulose, 0.5-2 parts of carbomer dry powder, 0.05-0.2 part of orchid essence and 30-40 parts of purified water.
2. The film patch for improving neck wrinkles as claimed in claim 1, wherein the film patch comprises, by weight, 5 parts of polyvinyl alcohol 1788, 5 parts of persimmon leaf powder, 1 part of sodium hyaluronate, 5 parts of glycerin, 0.3 part of titanium dioxide, 1.75 parts of polyvinylpyrrolidone, 1.25 parts of sodium carboxymethylcellulose, 1 part of carbomer dry powder, 0.1 part of orchid essence and 25 parts of purified water.
3. The film patch according to claim 1, wherein the film patch comprises, by weight, 4 parts of 1788 polyvinyl alcohol, 4 parts of persimmon leaf powder, 0.6 part of sodium hyaluronate, 4 parts of glycerin, 0.1 part of titanium dioxide, 1 part of polyvinylpyrrolidone, 1 part of sodium carboxymethylcellulose, 0.5 part of carbomer dry powder, 0.05 part of orchid essence, and 30 parts of purified water.
4. The film patch according to claim 1, wherein the film patch comprises, by weight, 6 parts of polyvinyl alcohol 1788, 6 parts of persimmon leaf powder, 1.2 parts of sodium hyaluronate, 6 parts of glycerin, 0.6 part of titanium dioxide, 3 parts of polyvinylpyrrolidone, 2 parts of sodium carboxymethylcellulose, 2 parts of carbomer dry powder, 0.2 part of orchid essence, and 40 parts of purified water.
5. The method for preparing the patch for improving neck wrinkles according to claim 1, which is carried out by the steps of:
weighing 4-6 parts of polyvinyl alcohol 1788, 4-6 parts of persimmon leaf powder, 0.6-1.2 parts of sodium hyaluronate, 4-6 parts of glycerol, 0.1-0.6 part of titanium dioxide, 1-3 parts of polyvinylpyrrolidone, 1-2 parts of sodium carboxymethylcellulose, 0.5-2 parts of carbomer dry powder, 0.05-0.2 part of orchid essence and 30-40 parts of purified water as raw materials in parts by weight;
secondly, dispersing the weighed carbomer dry powder into 10-15 parts of purified water, and standing for 18-24 hours to obtain a phase A;
thirdly, adding the weighed polyvinyl alcohol 1788 into 10-15 parts of purified water, ultrasonically dissolving for 20-40 min, then adding polyvinylpyrrolidone and sodium carboxymethylcellulose, and stirring at the rotating speed of 7000-9000 r/min for 20-40 min to obtain a phase B;
fourthly, adding the phase B into the phase A, mixing and stirring for 20min, adding the weighed persimmon leaf powder and sodium hyaluronate, mixing and stirring for 20-40 min, and obtaining a phase C;
and fifthly, adding the weighed titanium dioxide, glycerol and orchid essence into the rest purified water, mixing and stirring for 20-40 min, then adding into the phase C, and stirring for 20-40 min, thus finishing the preparation of the membrane patch for improving neck wrinkles.
6. The method for preparing a mask for improving neck wrinkles as claimed in claim 5, wherein in the first step, 5 parts of polyvinyl alcohol 1788, 5 parts of persimmon leaf powder, 0.8 parts of sodium hyaluronate, 5 parts of glycerin, 0.3 parts of titanium dioxide, 2 parts of polyvinylpyrrolidone, 1.5 parts of sodium carboxymethylcellulose, 1.5 parts of carbomer dry powder, 0.1 parts of orchid essence and 35 parts of purified water are weighed as raw materials.
7. The method for preparing a patch for improving neck wrinkles as claimed in claim 5, wherein the second step is dispersing in 15 parts of purified water, and standing for 20 h.
8. The method for preparing a patch for improving neck wrinkles as claimed in claim 5, wherein the third step is to add 10 parts of purified water, and ultrasonic dissolve for 30 min.
9. The method for preparing a film patch for improving neck wrinkles as claimed in claim 5, wherein the stirring is carried out at 8000r/min for 30min in the third step.
10. The method for preparing a patch for improving neck wrinkles as claimed in claim 5, wherein the mixing and stirring step of step five is performed for 30min, and then the mixture is added to phase C and stirred for 30 min.
CN202010797664.0A 2020-08-10 2020-08-10 Membrane patch for improving neck wrinkles and preparation method thereof Pending CN111728915A (en)

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Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20080036694A (en) * 2006-10-24 2008-04-29 황인수 Invention of crude cosmetic composition for anti-oxidative, bacterial activity, wrinkle, aging
JP2013075865A (en) * 2011-09-30 2013-04-25 Kuriya Yumi:Kk Hair dye composition
CN104257547A (en) * 2014-09-25 2015-01-07 张建瑞 Toothpaste containing persimmon leaf extracts
CN104490662A (en) * 2014-12-04 2015-04-08 云南人羞花化妆品有限公司 Pseudo-ginseng skin-caring and neck-protecting emulsion and preparation method thereof
CN106726977A (en) * 2016-12-28 2017-05-31 张学健 A kind of Honegsukle flower P.E facial mask
CN107041857A (en) * 2017-02-06 2017-08-15 柳州草子吉生物科技有限公司 A kind of smoothing wrinkle freckle-removing mask and preparation method thereof
CN107412134A (en) * 2017-08-28 2017-12-01 戈月芳 A kind of facial mask and preparation method thereof
CN107440963A (en) * 2017-08-23 2017-12-08 佛山市三水区嘉华化学研究院(普通合伙) A kind of persimmon leaf flavone with whitening anti-aging activity and its preparation method and application

Patent Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20080036694A (en) * 2006-10-24 2008-04-29 황인수 Invention of crude cosmetic composition for anti-oxidative, bacterial activity, wrinkle, aging
JP2013075865A (en) * 2011-09-30 2013-04-25 Kuriya Yumi:Kk Hair dye composition
CN104257547A (en) * 2014-09-25 2015-01-07 张建瑞 Toothpaste containing persimmon leaf extracts
CN104490662A (en) * 2014-12-04 2015-04-08 云南人羞花化妆品有限公司 Pseudo-ginseng skin-caring and neck-protecting emulsion and preparation method thereof
CN106726977A (en) * 2016-12-28 2017-05-31 张学健 A kind of Honegsukle flower P.E facial mask
CN107041857A (en) * 2017-02-06 2017-08-15 柳州草子吉生物科技有限公司 A kind of smoothing wrinkle freckle-removing mask and preparation method thereof
CN107440963A (en) * 2017-08-23 2017-12-08 佛山市三水区嘉华化学研究院(普通合伙) A kind of persimmon leaf flavone with whitening anti-aging activity and its preparation method and application
CN107412134A (en) * 2017-08-28 2017-12-01 戈月芳 A kind of facial mask and preparation method thereof

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
MAN,LEE JIN等: "Studies on Cosmeceutical Activity of Extracts of Persimmon Leaves", 《J INVEST COSMETOL》 *
王建新: "《化妆品植物原料大全》", 30 June 2012, 中国纺织出版社 *

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