CN111727850B - Culture medium for dendrobium officinale seedling culture and preparation method thereof - Google Patents
Culture medium for dendrobium officinale seedling culture and preparation method thereof Download PDFInfo
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- CN111727850B CN111727850B CN202010552899.3A CN202010552899A CN111727850B CN 111727850 B CN111727850 B CN 111727850B CN 202010552899 A CN202010552899 A CN 202010552899A CN 111727850 B CN111727850 B CN 111727850B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/20—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
- A01G24/28—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material containing peat, moss or sphagnum
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/10—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/20—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/20—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
- A01G24/22—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material containing plant material
- A01G24/23—Wood, e.g. wood chips or sawdust
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/20—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
- A01G24/22—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material containing plant material
- A01G24/27—Pulp, e.g. bagasse
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/30—Growth substrates; Culture media; Apparatus or methods therefor based on or containing synthetic organic compounds
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/20—Reduction of greenhouse gas [GHG] emissions in agriculture, e.g. CO2
- Y02P60/21—Dinitrogen oxide [N2O], e.g. using aquaponics, hydroponics or efficiency measures
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
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Abstract
The invention discloses a culture medium for dendrobium officinale seedling culture, which comprises the following raw materials: peat, bagasse, pine bark, carboxymethyl cellulose-montmorillonite intercalation compound, bacterial cellulose and microbial agent; the mass ratio of the peat, the bagasse, the pine bark, the carboxymethyl cellulose-montmorillonite intercalation compound, the bacterial cellulose and the microbial agent is (20-40): (5-15): (5-15): (2-6): (0.5-2): (0.5-2). The invention also discloses a preparation method of the culture medium for dendrobium officinale seedling culture. The culture medium provided by the invention can improve the transplanting survival rate of the dendrobium officinale tissue culture seedlings, can promote the growth and development of the seedlings, and has a good seedling culture effect.
Description
Technical Field
The invention relates to the technical field of dendrobium officinale seedling culture, and particularly relates to a culture medium for dendrobium officinale seedling culture and a preparation method thereof.
Background
Dendrobium officinale is an epiphytic herbaceous plant in the orchid family, is a traditional and rare traditional Chinese medicine in China, and has high medicinal and economic values. In recent years, with the discovery of health care values of the dendrobium officinale, such as tumor resistance, aging resistance, immunity improvement and the like, the market of the dendrobium officinale gradually expands, and the demand of dendrobium officinale seedlings is increasing day by day. In order to improve the adaptability of the dendrobium officinale seedlings to greenhouse cultivation, a method for transplanting the tissue culture seedlings into a culture medium for acclimatization can be adopted, wherein the culture medium is a key link influencing the acclimatization seedling raising effect. At present, the culture medium for dendrobium officinale seedling culture mainly comprises organic matters such as peat, bark and sawdust, inorganic clay such as vermiculite and a mixture thereof, has the defects of low seedling culture survival rate and slow growth, seriously influences the seedling culture efficiency and restricts the development of industry. Therefore, the development of the culture medium which can not only improve the transplanting survival rate of the dendrobium officinale tissue culture seedlings, but also promote the growth and development of the seedlings is an important research direction at present.
Disclosure of Invention
Based on the technical problems in the background art, the invention provides a culture medium for dendrobium officinale seedling culture and a preparation method thereof.
The invention provides a culture medium for dendrobium officinale seedling culture, which comprises the following raw materials: peat, bagasse, pine bark, carboxymethyl cellulose-montmorillonite intercalation compound, bacterial cellulose and microbial agent; the mass ratio of the peat, the bagasse, the pine bark, the carboxymethyl cellulose-montmorillonite intercalation compound, the bacterial cellulose and the microbial agent is (20-40): (5-15): (5-15): (2-6): (0.5-2): (0.5-2).
Preferably, the carboxymethyl cellulose-montmorillonite intercalated compound is prepared by taking carboxymethyl cellulose and montmorillonite as raw materials and adopting a solution intercalation method; preferably, the mass ratio of the carboxymethyl cellulose to the montmorillonite is (1-3): 10.
preferably, the preparation method of the carboxymethyl cellulose-montmorillonite intercalated compound comprises the following steps: mixing carboxymethyl cellulose solution and montmorillonite suspension uniformly, stirring and reacting for 5-10h at 55-70 deg.C and pH of 6-8, centrifuging, washing, and oven drying to obtain the final product; preferably, the mass fraction of the carboxymethyl cellulose solution is 1-2%, and the mass fraction of the montmorillonite suspension is 3-5%.
Preferably, the microbial agent is an EM microbial agent, and the effective viable count of the EM microbial agent is (1-10) multiplied by 10 9 CFU/g。
The preparation method of the culture medium for dendrobium officinale seedling culture comprises the following steps:
s1, weighing raw materials according to mass, uniformly mixing peat, bagasse, pine bark and a microbial agent, and fermenting to obtain a fermented material;
s2, adding the bacterial cellulose into water to be uniformly dispersed to obtain bacterial cellulose suspension;
s3, mixing the fermentation material with 30-50wt% of carboxymethyl cellulose-montmorillonite intercalation compound, adding 60-80wt% of bacterial cellulose suspension, and uniformly stirring to obtain a primary mixture;
and S4, mixing the primary mixture with the rest of the carboxymethyl cellulose-montmorillonite intercalation compound, adding the rest of the bacterial cellulose suspension, uniformly stirring, drying and sterilizing to obtain the antibacterial agent.
Preferably, the mass fraction of the bacterial cellulose suspension is 5-10%.
Preferably, in the step S1, the fermentation temperature is 37-42 ℃ and the fermentation time is 6-8 days.
Preferably, in the step S3, the stirring time is 3-5h; in the step S4, the stirring time is 1-3h.
Preferably, in the step S4, the sterilization method is high-pressure steam sterilization, and the sterilization condition is 121 ℃ and 15-20min.
The invention has the following beneficial effects:
the culture medium for dendrobium officinale seedling cultivation is prepared by compounding fermented peat, bagasse, pine bark and carboxymethyl cellulose-montmorillonite intercalated compound as a basic culture medium and compounding the basic culture medium with bacterial cellulose. The carboxymethyl cellulose-montmorillonite intercalated complex is compounded with fermented peat, bagasse and pine bark, so that nutrients such as sufficient mineral nutrients and organic matter nutrients in the growth and development process of the dendrobium officinale tissue culture seedling are provided, and the appropriate porosity and air permeability are provided, thereby facilitating the penetration and growth of the root system of the dendrobium officinale tissue culture seedling and promoting the growth and development of the tissue culture seedling; the carboxymethyl cellulose-montmorillonite intercalation compound is obtained by intercalating and modifying montmorillonite through carboxymethyl cellulose, and can effectively improve the water absorption, water retention and fertilizer retention performances of a matrix and promote the growth and development of tissue culture seedlings; carry out the intercalation to the montmorillonite through carboxymethyl cellulose and evenly introduce carboxymethyl cellulose in to cultivateing the matrix, add bacterial cellulose again, take place interact through the hydroxyl on bacterial cellulose surface and carboxymethyl cellulose's carboxyl, and bacterial cellulose and carboxymethyl cellulose's three-dimensional entanglement, be favorable to cultivateing the matrix and take place the winding with the root system of group culture seedling, can fix the root system better, thereby improve the survival rate of group culture seedling, and can improve the water-holding capacity of cultivateing the matrix, further promote the growth and development of group culture seedling. In the preparation process of the culture medium, the carboxymethyl cellulose-montmorillonite intercalation compound and the bacterial cellulose are added step by step and mixed, and a proper addition ratio is selected, so that the phenomena of agglomeration and uneven culture medium caused by excessive entanglement can be avoided, and the culture medium with high uniformity, good air permeability and strong water and fertilizer holding capacity is obtained. The culture medium provided by the invention can improve the transplanting survival rate of the dendrobium officinale tissue culture seedlings, can promote the growth and development of the seedlings, and has a good seedling culture effect.
Detailed Description
The technical solution of the present invention will be described in detail below with reference to specific examples.
Example 1
A culture medium for dendrobium officinale seedling culture comprises the following raw materials: peat, bagasse, pine bark, a carboxymethyl cellulose-montmorillonite intercalation compound, bacterial cellulose and an EM microbial inoculum, wherein the mass ratio of the peat, the bagasse, the pine bark, the carboxymethyl cellulose-montmorillonite intercalation compound, the bacterial cellulose to the EM microbial inoculum is 30:10:10:5:1:1.
the preparation method of the carboxymethyl cellulose-montmorillonite intercalation compound comprises the following steps: weighing 2 parts by mass of carboxymethyl cellulose and 10 parts by mass of montmorillonite, respectively preparing a carboxymethyl cellulose solution with the mass fraction of 1.5% and a montmorillonite suspension with the mass fraction of 4%, uniformly mixing, stirring and reacting for 6 hours under the conditions that the temperature is 60 ℃ and the pH is 7, centrifuging, washing and drying to obtain the montmorillonite-montmorillonite composite material; effective viable count of EM microbial inoculum is 5 multiplied by 10 9 CFU/g。
The preparation method of the culture medium for dendrobium officinale seedling culture comprises the following steps:
s1, weighing raw materials according to mass, uniformly mixing peat, bagasse, pine bark and EM microbial inoculum, and fermenting at 40 ℃ for 7 days to obtain a fermented material;
s2, adding the bacterial cellulose into water, and uniformly dispersing to obtain bacterial cellulose suspension with the mass fraction of 8%;
s3, mixing the fermentation material with 40wt% of carboxymethyl cellulose-montmorillonite intercalation compound, adding 75wt% of bacterial cellulose suspension, and stirring for 4 hours to obtain a primary mixture;
and S4, mixing the primary mixture with the rest of the carboxymethyl cellulose-montmorillonite intercalation compound, adding the rest of the bacterial cellulose suspension, stirring for 2 hours, drying, and sterilizing for 20 minutes at 121 ℃ to obtain the antibacterial agent.
Example 2
A culture medium for dendrobium officinale seedling raising comprises the following raw materials: peat, bagasse, pine bark, a carboxymethyl cellulose-montmorillonite intercalation compound, bacterial cellulose and an EM microbial inoculum, wherein the mass ratio of the peat, the bagasse, the pine bark, the carboxymethyl cellulose-montmorillonite intercalation compound, the bacterial cellulose to the EM microbial inoculum is 20:5:5:2:0.5:0.5.
the preparation method of the carboxymethyl cellulose-montmorillonite intercalation compound comprises the following steps: weighing 1 part by mass of carboxymethyl cellulose and 10 parts by mass of montmorillonite, respectively preparing a carboxymethyl cellulose solution with the mass fraction of 1% and a montmorillonite suspension with the mass fraction of 3-5%, uniformly mixing, stirring and reacting for 5 hours at the temperature of 55 ℃ and the pH value of 6, centrifuging, washing and drying to obtain the carboxymethyl cellulose montmorillonite-montmorillonite composite material; the effective viable count of the EM microbial inoculum is 1 multiplied by 10 9 CFU/g。
The preparation method of the culture medium for dendrobium officinale seedling culture comprises the following steps:
s1, weighing raw materials according to mass, uniformly mixing peat, bagasse, pine bark and an EM microbial inoculum, and then fermenting at 37 ℃ for 6 days to obtain a fermentation material;
s2, adding the bacterial cellulose into water, and uniformly dispersing to obtain a bacterial cellulose suspension with the mass fraction of 5%;
s3, mixing the fermentation material with 30wt% of carboxymethyl cellulose-montmorillonite intercalation compound, adding 60wt% of bacterial cellulose suspension, and stirring for 3 hours to obtain a primary mixture;
and S4, mixing the primary mixture with the rest of the carboxymethyl cellulose-montmorillonite intercalation compound, adding the rest of the bacterial cellulose suspension, stirring for 1h, drying, and sterilizing at 121 ℃ for 15min to obtain the antibacterial agent.
Example 3
A culture medium for dendrobium officinale seedling culture comprises the following raw materials: peat, bagasse, pine bark, a carboxymethyl cellulose-montmorillonite intercalation compound, bacterial cellulose and an EM microbial inoculum, wherein the mass ratio of the peat, the bagasse, the pine bark, the carboxymethyl cellulose-montmorillonite intercalation compound, the bacterial cellulose to the EM microbial inoculum is 40:15:15:6:2:2.
the preparation method of the carboxymethyl cellulose-montmorillonite intercalation compound comprises the following steps: weighing 3 parts by mass of carboxymethylPreparing a carboxymethyl cellulose solution with the mass fraction of 2% and a montmorillonite suspension with the mass fraction of 3-5% respectively from base cellulose and 10 parts by mass of montmorillonite, uniformly mixing, stirring and reacting for 10 hours at the temperature of 70 ℃ and the pH value of 8, centrifuging, washing and drying to obtain the montmorillonite-montmorillonite composite material; the effective viable count of EM microbial inoculum is 10 multiplied by 10 9 CFU/g。
The preparation method of the culture medium for dendrobium officinale seedling culture comprises the following steps:
s1, weighing raw materials according to mass, uniformly mixing peat, bagasse, pine bark and an EM microbial inoculum, and fermenting at 42 ℃ for 8 days to obtain a fermented material;
s2, adding the bacterial cellulose into water, and uniformly dispersing to obtain a bacterial cellulose suspension with the mass fraction of 10%;
s3, mixing the fermentation material with 50wt% of carboxymethyl cellulose-montmorillonite intercalation compound, adding 80wt% of bacterial cellulose suspension, and stirring for 5 hours to obtain a primary mixture;
and S4, mixing the primary mixture with the rest of the carboxymethyl cellulose-montmorillonite intercalation compound, adding the rest of the bacterial cellulose suspension, stirring for 3 hours, drying, and sterilizing for 20 minutes at 121 ℃ to obtain the antibacterial agent.
Comparative example 1
A culture medium for dendrobium officinale seedling culture comprises the following raw materials: peat, bagasse, pine bark, montmorillonite and EM microbial inoculum, wherein the mass ratio of the peat to the bagasse to the pine bark to the montmorillonite to the EM microbial inoculum is 30:10:10:6:1.
effective viable count of EM microbial inoculum is 5 multiplied by 10 9 CFU/g。
The preparation method of the culture medium for dendrobium officinale seedling culture comprises the following steps:
s1, weighing raw materials according to mass, uniformly mixing peat, bagasse, pine bark and an EM microbial inoculum, and then fermenting at 40 ℃ for 7 days to obtain a fermentation material;
s2, after the fermentation material and the montmorillonite are uniformly mixed, drying, and sterilizing at 121 deg.C for 20min.
Test examples
The dendrobium officinale tissue culture seedlings are subjected to seedling culture tests by using the culture mediums of examples 1-3 and comparative example 1 respectively, wherein the tissue culture seedlings used in the tests are all from the same fruit. Transplanting the tissue culture seedlings to a seedbed paved with a culture medium with the thickness of 5cm, watering the roots after transplanting until the medium is thoroughly watered, applying the seaweed water-soluble fertilizer once every 10 days, and spraying the bactericide and the insecticide once every 20 days. And after transplanting for 100 days, counting the survival rate, and sampling to determine the dry weight of the dendrobium officinale seedlings. The seedling test results are shown in table 1:
TABLE 1 test results of seedling growth
| Item | Example 1 | Example 2 | Example 3 | Comparative example 1 |
| Survival rate (%) | 93.6 | 90.5 | 92.3 | 85.8 |
| Dry weight (g) | 0.84 | 0.82 | 0.85 | 0.67 |
The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art should be considered to be within the technical scope of the present invention, and the technical solutions and the inventive concepts thereof according to the present invention should be equivalent or changed within the scope of the present invention.
Claims (7)
1. The culture medium for dendrobium officinale seedling culture is characterized by comprising the following raw materials: peat, bagasse, pine bark, carboxymethyl cellulose-montmorillonite intercalation compound, bacterial cellulose and microbial agent; the mass ratio of the peat, the bagasse, the pine bark, the carboxymethyl cellulose-montmorillonite intercalation compound, the bacterial cellulose and the microbial agent is (20-40): (5-15): (5-15): (2-6): (0.5-2): (0.5-2); the mass ratio of the carboxymethyl cellulose to the montmorillonite is (1-3): 10; the carboxymethyl cellulose-montmorillonite intercalated compound is prepared by taking carboxymethyl cellulose and montmorillonite as raw materials and adopting a solution intercalation method; the preparation method of the carboxymethyl cellulose-montmorillonite intercalated compound comprises the following steps: mixing carboxymethyl cellulose solution and montmorillonite suspension uniformly, stirring and reacting for 5-10h at 55-70 deg.C and pH of 6-8, centrifuging, washing, and oven drying to obtain the final product; the mass fraction of the carboxymethyl cellulose solution is 1-2%, and the mass fraction of the montmorillonite suspension is 3-5%.
2. The culture medium for raising dendrobium officinale seedlings according to claim 1, wherein the microbial agent is an EM microbial agent, and the effective viable count of the EM microbial agent is (1-10) x 10 9 CFU/g。
3. A preparation method of the culture medium for dendrobium officinale seedling culture according to claim 1 or 2, characterized by comprising the following steps:
s1, weighing raw materials according to mass, uniformly mixing peat, bagasse, pine bark and a microbial agent, and fermenting to obtain a fermented material;
s2, adding the bacterial cellulose into water, and uniformly dispersing to obtain a bacterial cellulose suspension;
s3, mixing the fermentation material with 30-50wt% of carboxymethyl cellulose-montmorillonite intercalation compound, adding 60-80wt% of bacterial cellulose suspension, and uniformly stirring to obtain a primary mixture;
and S4, mixing the primary mixture with the rest part of the carboxymethyl cellulose-montmorillonite intercalation compound in the S3, adding the rest part of the bacterial cellulose suspension in the S3, uniformly stirring, drying and sterilizing to obtain the antibacterial cellulose.
4. The preparation method of the culture medium for dendrobium officinale seedling culture according to claim 3, wherein the mass fraction of the bacterial cellulose suspension is 5-10%.
5. The preparation method of the culture medium for dendrobium officinale seedling culture according to claim 3, wherein in the step S1, the fermentation temperature is 37-42 ℃ and the fermentation time is 6-8 days.
6. The preparation method of the culture medium for dendrobium officinale seedling culture according to claim 3, wherein in the step S3, the stirring time is 3-5h; in the step S4, the stirring time is 1-3h.
7. The preparation method of the culture medium for dendrobium officinale seedling culture according to claim 3, wherein in the step S4, the sterilization method is high-pressure steam sterilization, and the sterilization condition is 121 ℃ and 15-20min.
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| CN105724209A (en) * | 2016-02-22 | 2016-07-06 | 云南兰京农业发展有限责任公司 | Novel dendrobe culture medium |
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