CN111718929B - 利用环形rna进行蛋白翻译及其应用 - Google Patents

利用环形rna进行蛋白翻译及其应用 Download PDF

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CN111718929B
CN111718929B CN201910214234.9A CN201910214234A CN111718929B CN 111718929 B CN111718929 B CN 111718929B CN 201910214234 A CN201910214234 A CN 201910214234A CN 111718929 B CN111718929 B CN 111718929B
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translation initiation
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protein
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initiation factor
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CN111718929A (zh
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王泽峰
杨赟
樊晓娟
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Shanghai Huanma Biopharmaceutical Co ltd
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Shanghai Institute of Nutrition and Health of CAS
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Priority to CN201910214234.9A priority patent/CN111718929B/zh
Priority to CN202410975296.2A priority patent/CN118834867A/zh
Priority to PCT/CN2020/077026 priority patent/WO2020186991A1/zh
Priority to CN202080036818.2A priority patent/CN113825837A/zh
Priority to EP20773279.3A priority patent/EP3943601A4/en
Priority to CN202410093532.8A priority patent/CN117965533A/zh
Priority to CN202410975341.4A priority patent/CN118834868A/zh
Priority to US17/440,774 priority patent/US20220177898A1/en
Priority to JP2021559486A priority patent/JP7297331B2/ja
Publication of CN111718929A publication Critical patent/CN111718929A/zh
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Priority to JP2023094068A priority patent/JP7673989B2/ja
Priority to JP2024209710A priority patent/JP2025029080A/ja
Priority to JP2024209709A priority patent/JP2025029079A/ja
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    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
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    • C12N2840/00—Vectors comprising a special translation-regulating system
    • C12N2840/20—Vectors comprising a special translation-regulating system translation of more than one cistron
    • C12N2840/203—Vectors comprising a special translation-regulating system translation of more than one cistron having an IRES
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    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione

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CN201910214234.9A 2019-03-20 2019-03-20 利用环形rna进行蛋白翻译及其应用 Active CN111718929B (zh)

Priority Applications (13)

Application Number Priority Date Filing Date Title
CN201910214234.9A CN111718929B (zh) 2019-03-20 2019-03-20 利用环形rna进行蛋白翻译及其应用
CN202211124976.0A CN115806976A (zh) 2019-03-20 2019-03-20 利用环形rna进行蛋白翻译及其应用
JP2021559486A JP7297331B2 (ja) 2019-03-20 2020-02-27 環状ｒｎａを使用したタンパク質翻訳およびその応用
CN202080036818.2A CN113825837A (zh) 2019-03-20 2020-02-27 利用环形rna进行蛋白翻译及其应用
EP20773279.3A EP3943601A4 (en) 2019-03-20 2020-02-27 TRANSLATION OF PROTEINS USING CIRCULAR RNA AND THEIR USE
CN202410093532.8A CN117965533A (zh) 2019-03-20 2020-02-27 利用环形rna进行蛋白翻译及其应用
CN202410975296.2A CN118834867A (zh) 2019-03-20 2020-02-27 利用环形rna进行蛋白翻译及其应用
US17/440,774 US20220177898A1 (en) 2019-03-20 2020-02-27 Protein translation using circular rna and application thereof
PCT/CN2020/077026 WO2020186991A1 (zh) 2019-03-20 2020-02-27 利用环形rna进行蛋白翻译及其应用
CN202410975341.4A CN118834868A (zh) 2019-03-20 2020-02-27 利用环形rna进行蛋白翻译及其应用
JP2023094068A JP7673989B2 (ja) 2019-03-20 2023-06-07 環状ｒｎａを使用したタンパク質翻訳およびその応用
JP2024209710A JP2025029080A (ja) 2019-03-20 2024-12-02 環状ｒｎａを使用したタンパク質翻訳およびその応用
JP2024209709A JP2025029079A (ja) 2019-03-20 2024-12-02 環状ｒｎａを使用したタンパク質翻訳およびその応用

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CN201910214234.9A CN111718929B (zh) 2019-03-20 2019-03-20 利用环形rna进行蛋白翻译及其应用

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CN111718929B true CN111718929B (zh) 2022-10-18

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CN201910214234.9A Active CN111718929B (zh) 2019-03-20 2019-03-20 利用环形rna进行蛋白翻译及其应用
CN202211124976.0A Pending CN115806976A (zh) 2019-03-20 2019-03-20 利用环形rna进行蛋白翻译及其应用
CN202410975296.2A Pending CN118834867A (zh) 2019-03-20 2020-02-27 利用环形rna进行蛋白翻译及其应用
CN202410093532.8A Pending CN117965533A (zh) 2019-03-20 2020-02-27 利用环形rna进行蛋白翻译及其应用
CN202080036818.2A Pending CN113825837A (zh) 2019-03-20 2020-02-27 利用环形rna进行蛋白翻译及其应用
CN202410975341.4A Pending CN118834868A (zh) 2019-03-20 2020-02-27 利用环形rna进行蛋白翻译及其应用

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CN202211124976.0A Pending CN115806976A (zh) 2019-03-20 2019-03-20 利用环形rna进行蛋白翻译及其应用
CN202410975296.2A Pending CN118834867A (zh) 2019-03-20 2020-02-27 利用环形rna进行蛋白翻译及其应用
CN202410093532.8A Pending CN117965533A (zh) 2019-03-20 2020-02-27 利用环形rna进行蛋白翻译及其应用
CN202080036818.2A Pending CN113825837A (zh) 2019-03-20 2020-02-27 利用环形rna进行蛋白翻译及其应用
CN202410975341.4A Pending CN118834868A (zh) 2019-03-20 2020-02-27 利用环形rna进行蛋白翻译及其应用

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US (1) US20220177898A1 (https=)
EP (1) EP3943601A4 (https=)
JP (4) JP7297331B2 (https=)
CN (6) CN111718929B (https=)
WO (1) WO2020186991A1 (https=)

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CN112608946B (zh) * 2021-01-17 2023-07-28 楷拓生物科技(苏州)有限公司 一种环状rna载体及其在疫苗中的应用
ES2994006T3 (en) 2021-09-17 2025-01-16 Flagship Pioneering Innovations Vi Llc Compositions and methods for producing circular polyribonucleotides
CN121752588A (zh) 2023-08-29 2026-03-27 上海环码生物医药有限公司 编码vegf多肽的环状rna、制剂和使用方法
WO2025077734A1 (en) * 2023-10-09 2025-04-17 Shanghai Circode Biomed Co., Ltd. Constructs and methods for preparing circular rnas and uses thereof

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2012177639A2 (en) * 2011-06-22 2012-12-27 Alnylam Pharmaceuticals, Inc. Bioprocessing and bioproduction using avian cell lines
CN105985978A (zh) * 2015-03-06 2016-10-05 中国科学院上海生命科学研究院 一种新型rna环化表达载体的构建及其应用
CN109423497A (zh) * 2017-08-31 2019-03-05 康码（上海）生物科技有限公司 增强蛋白质合成效率的rna元件

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
BRPI0109522B8 (pt) * 2000-03-28 2017-04-11 Farm Bioindustries Pty Ltd construto genético linear e métodos para gerar planta transgênica ou progênie da mesma resistente a vírus ssdna e planta ou parte da mesma geneticamente modificada
AU2001250572A1 (en) * 2000-04-07 2001-10-23 Epigenomics Ag Detection of single nucleotide polymorphisms (snp's) and cytosine-methylations
US20080107600A1 (en) * 2003-02-13 2008-05-08 Taiichi Katayama Gene Marker And Composition For Diagnosis And Treatment Of Neurological Disorders And Diseases And Use Of The Same
WO2009135056A2 (en) 2008-04-30 2009-11-05 Monsanto Technology Llc Recombinant dna vectors for expression of human prolactin antagonists
US20170298347A1 (en) * 2016-02-03 2017-10-19 Beth Israel Deaconess Medical Center NOVEL FUSION-CIRCULAR RNAs AND USES THEREOF
CN109022478A (zh) * 2017-06-09 2018-12-18 康码（上海）生物科技有限公司 一种用于高通量体外蛋白质合成的dna元件
CN108690139B (zh) * 2017-07-31 2019-03-15 康码（上海）生物科技有限公司 新型融合蛋白的制备及其在提高蛋白质合成的应用
CN109423496B (zh) * 2017-08-31 2023-09-15 康码（上海）生物科技有限公司 一种细胞中内源性表达rna聚合酶的核酸构建物
IL316195A (en) * 2017-12-15 2024-12-01 Flagship Pioneering Innovations Vi Llc Preparations containing circular polyribonucleotides and uses thereof
CN109971775B (zh) * 2017-12-27 2021-12-28 康码（上海）生物科技有限公司 一种核酸构建物及其调控蛋白合成的方法

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2012177639A2 (en) * 2011-06-22 2012-12-27 Alnylam Pharmaceuticals, Inc. Bioprocessing and bioproduction using avian cell lines
CN105985978A (zh) * 2015-03-06 2016-10-05 中国科学院上海生命科学研究院 一种新型rna环化表达载体的构建及其应用
CN109423497A (zh) * 2017-08-31 2019-03-05 康码（上海）生物科技有限公司 增强蛋白质合成效率的rna元件

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
《Extensive translation of circular RNAs driven by N-6-methyladenosine》;Yang Yun等;《CELL RESEARCH》;20170531;第27卷(第5期);参见第631页，图3A *
《Pervasive translation of circular RNAs driven by short IRES-like elements》;Xiaojuan Fan等;《biorxiv-https://www.biorxiv.org/content/10.1101/473207v1?versioned=true》;20181118;第2页摘要、第4页2段 *
Xiaojuan Fan等.《Pervasive translation of circular RNAs driven by short IRES-like elements》.《biorxiv-https://www.biorxiv.org/content/10.1101/473207v1?versioned=true》.2018,第1-40页. *

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JP7673989B2 (ja) 2025-05-09
JP2025029080A (ja) 2025-03-05
EP3943601A1 (en) 2022-01-26
WO2020186991A1 (zh) 2020-09-24
CN117965533A (zh) 2024-05-03
EP3943601A4 (en) 2022-12-14
CN115806976A (zh) 2023-03-17
CN118834868A (zh) 2024-10-25
CN111718929A (zh) 2020-09-29
CN113825837A (zh) 2021-12-21
JP7297331B2 (ja) 2023-06-26
US20220177898A1 (en) 2022-06-09
JP2025029079A (ja) 2025-03-05
JP2022528252A (ja) 2022-06-09
CN118834867A (zh) 2024-10-25
JP2023116620A (ja) 2023-08-22

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