CN113825837A - 利用环形rna进行蛋白翻译及其应用 - Google Patents

利用环形rna进行蛋白翻译及其应用 Download PDF

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CN113825837A
CN113825837A CN202080036818.2A CN202080036818A CN113825837A CN 113825837 A CN113825837 A CN 113825837A CN 202080036818 A CN202080036818 A CN 202080036818A CN 113825837 A CN113825837 A CN 113825837A
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equal
translation initiation
construct
protein
sequence
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王泽峰
杨赟
樊晓娟
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Shanghai Huanma Biopharmaceutical Co ltd
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Shanghai Institute of Nutrition and Health of CAS
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/111—General methods applicable to biologically active non-coding nucleic acids
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2840/00—Vectors comprising a special translation-regulating system
    • C12N2840/20—Vectors comprising a special translation-regulating system translation of more than one cistron
    • C12N2840/203—Vectors comprising a special translation-regulating system translation of more than one cistron having an IRES
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione

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  • Health & Medical Sciences (AREA)
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Abstract

提供了一种环形RNA构建物及其应用，所述环形RNA构建物从5’‑3’方向具有式I所示的结构：TI‑Z1‑Z2(I)，式中，TI为翻译起始元件；Z1为表达外源蛋白的表达盒；Z2为无或其他元件。

Description

PCT国内申请，说明书已公开。

Claims (10)

  1. PCT国内申请，权利要求书已公开。
CN202080036818.2A 2019-03-20 2020-02-27 利用环形rna进行蛋白翻译及其应用 Pending CN113825837A (zh)

Priority Applications (1)

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CN202410093532.8A CN117965533A (zh) 2019-03-20 2020-02-27 利用环形rna进行蛋白翻译及其应用

Applications Claiming Priority (3)

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CN201910214234.9A CN111718929B (zh) 2019-03-20 2019-03-20 利用环形rna进行蛋白翻译及其应用
CN2019102142349 2019-03-20
PCT/CN2020/077026 WO2020186991A1 (zh) 2019-03-20 2020-02-27 利用环形rna进行蛋白翻译及其应用

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CN202410093532.8A Division CN117965533A (zh) 2019-03-20 2020-02-27 利用环形rna进行蛋白翻译及其应用
CN202410975296.2A Division CN118834867A (zh) 2019-03-20 2020-02-27 利用环形rna进行蛋白翻译及其应用
CN202410975341.4A Division CN118834868A (zh) 2019-03-20 2020-02-27 利用环形rna进行蛋白翻译及其应用

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CN113825837A true CN113825837A (zh) 2021-12-21

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CN201910214234.9A Active CN111718929B (zh) 2019-03-20 2019-03-20 利用环形rna进行蛋白翻译及其应用
CN202410093532.8A Pending CN117965533A (zh) 2019-03-20 2020-02-27 利用环形rna进行蛋白翻译及其应用
CN202080036818.2A Pending CN113825837A (zh) 2019-03-20 2020-02-27 利用环形rna进行蛋白翻译及其应用

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CN201910214234.9A Active CN111718929B (zh) 2019-03-20 2019-03-20 利用环形rna进行蛋白翻译及其应用
CN202410093532.8A Pending CN117965533A (zh) 2019-03-20 2020-02-27 利用环形rna进行蛋白翻译及其应用

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US (1) US20220177898A1 (zh)
EP (1) EP3943601A4 (zh)
JP (2) JP7297331B2 (zh)
CN (4) CN115806976A (zh)
WO (1) WO2020186991A1 (zh)

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Publication number Priority date Publication date Assignee Title
CN112608946B (zh) * 2021-01-17 2023-07-28 楷拓生物科技(苏州)有限公司 一种环状rna载体及其在疫苗中的应用

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109423496A (zh) * 2017-08-31 2019-03-05 康码（上海）生物科技有限公司 一种细胞中内源性表达rna聚合酶的核酸构建物
CN109423497A (zh) * 2017-08-31 2019-03-05 康码（上海）生物科技有限公司 增强蛋白质合成效率的rna元件

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PT1282697E (pt) * 2000-03-28 2008-02-01 Univ Queensland Construção capaz de libertação na forma circular fechada a partir de uma grande sequência nucleotídica permitindo a expressão específica do local e/ou a expressão regulada durante o desenvolvimento de sequências genéticas seleccionadas
WO2012177639A2 (en) * 2011-06-22 2012-12-27 Alnylam Pharmaceuticals, Inc. Bioprocessing and bioproduction using avian cell lines
CN105985978B (zh) * 2015-03-06 2020-02-14 中国科学院上海生命科学研究院 一种新型rna环化表达载体的构建及其应用
CN111819185A (zh) * 2017-12-15 2020-10-23 旗舰创业创新第六有限责任公司 包含环状多核糖核苷酸的组合物及其用途
CN109971775B (zh) * 2017-12-27 2021-12-28 康码（上海）生物科技有限公司 一种核酸构建物及其调控蛋白合成的方法

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109423496A (zh) * 2017-08-31 2019-03-05 康码（上海）生物科技有限公司 一种细胞中内源性表达rna聚合酶的核酸构建物
CN109423497A (zh) * 2017-08-31 2019-03-05 康码（上海）生物科技有限公司 增强蛋白质合成效率的rna元件

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
XIAOJUAN FAN 等: "Pervasive translation of circular RNAs driven by short IRES-like elements.", 《BIORXIV》 *
YANG YUN等: "Extensive translation of circular RNAs driven by N-6-methyladenosine.", 《CELL RESEARCH》 *

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Publication number Publication date
JP7297331B2 (ja) 2023-06-26
CN111718929B (zh) 2022-10-18
JP2023116620A (ja) 2023-08-22
CN115806976A (zh) 2023-03-17
US20220177898A1 (en) 2022-06-09
CN117965533A (zh) 2024-05-03
CN111718929A (zh) 2020-09-29
EP3943601A4 (en) 2022-12-14
EP3943601A1 (en) 2022-01-26
WO2020186991A1 (zh) 2020-09-24
JP2022528252A (ja) 2022-06-09

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