JP7297331B2 - 環状ｒｎａを使用したタンパク質翻訳およびその応用 - Google Patents

環状ｒｎａを使用したタンパク質翻訳およびその応用 Download PDF

Info

Publication number
JP7297331B2
JP7297331B2 JP2021559486A JP2021559486A JP7297331B2 JP 7297331 B2 JP7297331 B2 JP 7297331B2 JP 2021559486 A JP2021559486 A JP 2021559486A JP 2021559486 A JP2021559486 A JP 2021559486A JP 7297331 B2 JP7297331 B2 JP 7297331B2
Authority
JP
Japan
Prior art keywords
translation initiation
circular rna
protein
rna construct
translation
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
JP2021559486A
Other languages
English (en)
Japanese (ja)
Other versions
JP2022528252A5 (https=
JPWO2020186991A5 (https=
JP2022528252A (ja
Inventor
ヅァフォン ワン
ユン ヤン
シャオジュエン ファン
Original Assignee
シャンハイ インスティチュート オブ ニュートリション アンド ヘルス，チャイニーズ アカデミー オブ サイエンシーズ
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by シャンハイ インスティチュート オブ ニュートリション アンド ヘルス，チャイニーズ アカデミー オブ サイエンシーズ filed Critical シャンハイ インスティチュート オブ ニュートリション アンド ヘルス，チャイニーズ アカデミー オブ サイエンシーズ
Publication of JP2022528252A publication Critical patent/JP2022528252A/ja
Publication of JP2022528252A5 publication Critical patent/JP2022528252A5/ja
Publication of JPWO2020186991A5 publication Critical patent/JPWO2020186991A5/ja
Priority to JP2023094068A priority Critical patent/JP7673989B2/ja
Application granted granted Critical
Publication of JP7297331B2 publication Critical patent/JP7297331B2/ja
Priority to JP2024209710A priority patent/JP2025029080A/ja
Priority to JP2024209709A priority patent/JP2025029079A/ja
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/111—General methods applicable to biologically active non-coding nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2840/00—Vectors comprising a special translation-regulating system
    • C12N2840/20—Vectors comprising a special translation-regulating system translation of more than one cistron
    • C12N2840/203—Vectors comprising a special translation-regulating system translation of more than one cistron having an IRES
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
JP2021559486A 2019-03-20 2020-02-27 環状ｒｎａを使用したタンパク質翻訳およびその応用 Active JP7297331B2 (ja)

Priority Applications (3)

Application Number Priority Date Filing Date Title
JP2023094068A JP7673989B2 (ja) 2019-03-20 2023-06-07 環状ｒｎａを使用したタンパク質翻訳およびその応用
JP2024209710A JP2025029080A (ja) 2019-03-20 2024-12-02 環状ｒｎａを使用したタンパク質翻訳およびその応用
JP2024209709A JP2025029079A (ja) 2019-03-20 2024-12-02 環状ｒｎａを使用したタンパク質翻訳およびその応用

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
CN201910214234.9 2019-03-20
CN201910214234.9A CN111718929B (zh) 2019-03-20 2019-03-20 利用环形rna进行蛋白翻译及其应用
PCT/CN2020/077026 WO2020186991A1 (zh) 2019-03-20 2020-02-27 利用环形rna进行蛋白翻译及其应用

Related Child Applications (1)

Application Number Title Priority Date Filing Date
JP2023094068A Division JP7673989B2 (ja) 2019-03-20 2023-06-07 環状ｒｎａを使用したタンパク質翻訳およびその応用

Publications (4)

Publication Number Publication Date
JP2022528252A JP2022528252A (ja) 2022-06-09
JP2022528252A5 JP2022528252A5 (https=) 2022-09-13
JPWO2020186991A5 JPWO2020186991A5 (https=) 2022-09-13
JP7297331B2 true JP7297331B2 (ja) 2023-06-26

Family

ID=72519626

Family Applications (4)

Application Number Title Priority Date Filing Date
JP2021559486A Active JP7297331B2 (ja) 2019-03-20 2020-02-27 環状ｒｎａを使用したタンパク質翻訳およびその応用
JP2023094068A Active JP7673989B2 (ja) 2019-03-20 2023-06-07 環状ｒｎａを使用したタンパク質翻訳およびその応用
JP2024209709A Pending JP2025029079A (ja) 2019-03-20 2024-12-02 環状ｒｎａを使用したタンパク質翻訳およびその応用
JP2024209710A Pending JP2025029080A (ja) 2019-03-20 2024-12-02 環状ｒｎａを使用したタンパク質翻訳およびその応用

Family Applications After (3)

Application Number Title Priority Date Filing Date
JP2023094068A Active JP7673989B2 (ja) 2019-03-20 2023-06-07 環状ｒｎａを使用したタンパク質翻訳およびその応用
JP2024209709A Pending JP2025029079A (ja) 2019-03-20 2024-12-02 環状ｒｎａを使用したタンパク質翻訳およびその応用
JP2024209710A Pending JP2025029080A (ja) 2019-03-20 2024-12-02 環状ｒｎａを使用したタンパク質翻訳およびその応用

Country Status (5)

Country Link
US (1) US20220177898A1 (https=)
EP (1) EP3943601A4 (https=)
JP (4) JP7297331B2 (https=)
CN (6) CN111718929B (https=)
WO (1) WO2020186991A1 (https=)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2025045142A1 (en) 2023-08-29 2025-03-06 Shanghai Circode Biomed Co., Ltd. Circular rna encoding vegf polypeptides, formulations, and methods of uses

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112608946B (zh) * 2021-01-17 2023-07-28 楷拓生物科技(苏州)有限公司 一种环状rna载体及其在疫苗中的应用
ES2994006T3 (en) 2021-09-17 2025-01-16 Flagship Pioneering Innovations Vi Llc Compositions and methods for producing circular polyribonucleotides
WO2025077734A1 (en) * 2023-10-09 2025-04-17 Shanghai Circode Biomed Co., Ltd. Constructs and methods for preparing circular rnas and uses thereof

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109423497A (zh) 2017-08-31 2019-03-05 康码（上海）生物科技有限公司 增强蛋白质合成效率的rna元件

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
BRPI0109522B8 (pt) * 2000-03-28 2017-04-11 Farm Bioindustries Pty Ltd construto genético linear e métodos para gerar planta transgênica ou progênie da mesma resistente a vírus ssdna e planta ou parte da mesma geneticamente modificada
AU2001250572A1 (en) * 2000-04-07 2001-10-23 Epigenomics Ag Detection of single nucleotide polymorphisms (snp's) and cytosine-methylations
US20080107600A1 (en) * 2003-02-13 2008-05-08 Taiichi Katayama Gene Marker And Composition For Diagnosis And Treatment Of Neurological Disorders And Diseases And Use Of The Same
WO2009135056A2 (en) 2008-04-30 2009-11-05 Monsanto Technology Llc Recombinant dna vectors for expression of human prolactin antagonists
WO2012177639A2 (en) * 2011-06-22 2012-12-27 Alnylam Pharmaceuticals, Inc. Bioprocessing and bioproduction using avian cell lines
CN105985978B (zh) * 2015-03-06 2020-02-14 中国科学院上海生命科学研究院 一种新型rna环化表达载体的构建及其应用
US20170298347A1 (en) * 2016-02-03 2017-10-19 Beth Israel Deaconess Medical Center NOVEL FUSION-CIRCULAR RNAs AND USES THEREOF
CN109022478A (zh) * 2017-06-09 2018-12-18 康码（上海）生物科技有限公司 一种用于高通量体外蛋白质合成的dna元件
CN108690139B (zh) * 2017-07-31 2019-03-15 康码（上海）生物科技有限公司 新型融合蛋白的制备及其在提高蛋白质合成的应用
CN109423496B (zh) * 2017-08-31 2023-09-15 康码（上海）生物科技有限公司 一种细胞中内源性表达rna聚合酶的核酸构建物
IL316195A (en) * 2017-12-15 2024-12-01 Flagship Pioneering Innovations Vi Llc Preparations containing circular polyribonucleotides and uses thereof
CN109971775B (zh) * 2017-12-27 2021-12-28 康码（上海）生物科技有限公司 一种核酸构建物及其调控蛋白合成的方法

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109423497A (zh) 2017-08-31 2019-03-05 康码（上海）生物科技有限公司 增强蛋白质合成效率的rna元件

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2025045142A1 (en) 2023-08-29 2025-03-06 Shanghai Circode Biomed Co., Ltd. Circular rna encoding vegf polypeptides, formulations, and methods of uses

Also Published As

Publication number Publication date
JP7673989B2 (ja) 2025-05-09
CN111718929B (zh) 2022-10-18
JP2025029080A (ja) 2025-03-05
EP3943601A1 (en) 2022-01-26
WO2020186991A1 (zh) 2020-09-24
CN117965533A (zh) 2024-05-03
EP3943601A4 (en) 2022-12-14
CN115806976A (zh) 2023-03-17
CN118834868A (zh) 2024-10-25
CN111718929A (zh) 2020-09-29
CN113825837A (zh) 2021-12-21
US20220177898A1 (en) 2022-06-09
JP2025029079A (ja) 2025-03-05
JP2022528252A (ja) 2022-06-09
CN118834867A (zh) 2024-10-25
JP2023116620A (ja) 2023-08-22

Similar Documents

Publication Publication Date Title
JP7673989B2 (ja) 環状ｒｎａを使用したタンパク質翻訳およびその応用
KR102695752B1 (ko) Aav 벡터 및 프로모터의 단일 세포 전사체 기반 개발을 위한 방법 및 재료
KR20090053893A (ko) 전사를 증가시키기 위한 기질부착부위(ｍａｒｓ) 및 그의 용도
EP3686282B1 (en) Promoter
IL323774A (en) Mutation effect and activity profile mapping system and method
CN109628489B (zh) 一种提高cho细胞重组蛋白表达水平的方法及其应用，表达载体、表达系统及其制备方法
CN102392047B (zh) 一种适用于哺乳动物细胞的双顺反子表达载体及其应用
WO2024199219A1 (en) Isolated transposase and use thereof
CN114901828A (zh) 一种哺乳动物表达系统使用的高表达载体
JP6384685B2 (ja) 組換えタンパク質の発現増進のための遺伝子断片を含むベクター及びその用途
CN109988777A (zh) 谷氨酰胺合成酶基因以及应用
TW201819631A (zh) Ｈｓｐａ５基因的啓動子
Dangerfield et al. Enhancement of the StreptoTag method for isolation of endogenously expressed proteins with complex RNA binding targets
HK40057766A (en) Protein translation using circular rna and application thereof
CN114891829B (zh) 一种肝特异性游离型表达载体和基因治疗载体及其应用
JP6372792B2 (ja) 酵母における目的遺伝子の発現システム及びその利用
EP1957660B1 (en) Materials and methods to increase peptide chain expression
EP4508202A1 (en) Isolated transposase and use thereof
CN116814611A (zh) 增强胞内分子检测灵敏度的人工核糖核酸工具的构建方法
CN118786222A (zh) 具有选择标志物的多核苷酸
CN121006371A (zh) Cre重组酶的编码核酸及其应用
HK40034561A (en) Promoter
HK40034561B (en) Promoter

Legal Events

Date Code Title Description
A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20211122

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20220404

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20220901

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20221108

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20230208

TRDD Decision of grant or rejection written
A01 Written decision to grant a patent or to grant a registration (utility model)

Free format text: JAPANESE INTERMEDIATE CODE: A01

Effective date: 20230509

A61 First payment of annual fees (during grant procedure)

Free format text: JAPANESE INTERMEDIATE CODE: A61

Effective date: 20230607

R150 Certificate of patent or registration of utility model

Ref document number: 7297331

Country of ref document: JP

Free format text: JAPANESE INTERMEDIATE CODE: R150

S111 Request for change of ownership or part of ownership

Free format text: JAPANESE INTERMEDIATE CODE: R313113

R350 Written notification of registration of transfer

Free format text: JAPANESE INTERMEDIATE CODE: R350