CN111713494A - Preparation method of efficient environment-friendly biological herbicide - Google Patents
Preparation method of efficient environment-friendly biological herbicide Download PDFInfo
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- CN111713494A CN111713494A CN202010629149.1A CN202010629149A CN111713494A CN 111713494 A CN111713494 A CN 111713494A CN 202010629149 A CN202010629149 A CN 202010629149A CN 111713494 A CN111713494 A CN 111713494A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/32—Ingredients for reducing the noxious effect of the active substances to organisms other than pests, e.g. toxicity reducing compositions, self-destructing compositions
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N33/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic nitrogen compounds
- A01N33/16—Biocides, pest repellants or attractants, or plant growth regulators containing organic nitrogen compounds containing nitrogen-to-oxygen bonds
- A01N33/18—Nitro compounds
- A01N33/20—Nitro compounds containing oxygen or sulfur attached to the carbon skeleton containing the nitro group
- A01N33/22—Nitro compounds containing oxygen or sulfur attached to the carbon skeleton containing the nitro group having at least one oxygen or sulfur atom and at least one nitro group directly attached to the same aromatic ring system
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/22—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom rings with more than six members
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/34—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom
- A01N43/40—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom six-membered rings
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/64—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with three nitrogen atoms as the only ring hetero atoms
- A01N43/66—1,3,5-Triazines, not hydrogenated and not substituted at the ring nitrogen atoms
- A01N43/68—1,3,5-Triazines, not hydrogenated and not substituted at the ring nitrogen atoms with two or three nitrogen atoms directly attached to ring carbon atoms
- A01N43/70—Diamino—1,3,5—triazines with only one oxygen, sulfur or halogen atom or only one cyano, thiocyano (—SCN), cyanato (—OCN) or azido (—N3) group directly attached to a ring carbon atom
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N51/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds having the sequences of atoms O—N—S, X—O—S, N—N—S, O—N—N or O-halogen, regardless of the number of bonds each atom has and with no atom of these sequences forming part of a heterocyclic ring
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/20—Bacteria; Substances produced thereby or obtained therefrom
- A01N63/22—Bacillus
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/30—Microbial fungi; Substances produced thereby or obtained therefrom
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
Abstract
The invention discloses a preparation method of a high-efficiency environment-friendly biological herbicide, belonging to the technical field of herbicides. A preparation method of a high-efficiency environment-friendly biological herbicide comprises the following steps: s1: preparing a herbicide A; the herbicide agent A comprises: atrazine, anthrax bacteria, brassinolide, fluazifop-p-butyl, Fusarium pachydenum, and wetting agent; s2: preparing a herbicide B; the herbicide agent B comprises: sodium diquat, compound sodium nitrophenolate and paecilomyces lilacinus fungicide; s3: preparing a herbicide C; the herbicide agent C includes: calcium oxide, kudzu, biomass carbon; the atrazine can be added into the herbicide to effectively inhibit the growth and the germination of the crabgrass seeds, the phytotoxicity of the atrazine on crops can be reduced and the growth of the crops can be promoted by adding the sodium diurethane into the herbicide, and the phytotoxicity of the atrazine can be reduced, the sodium diurethane can be decomposed and the toxicity of the soil can be reduced by adding the calcium oxide into the herbicide.
Description
Technical Field
The invention relates to the technical field of herbicides, and in particular relates to a preparation method of a high-efficiency environment-friendly biological herbicide.
Background
Crops are various plants cultivated in agriculture, including two main types of grain crops and economic crops: the relation between people and food is expressed, the reasonable diet collocation can bring health to people, the growth of crops and scientific technological production technology can not be left, and the novel industrially manufactured mechanical equipment can assist agricultural production.
Weeds are often required to appear in the growth process of crops, and the weeds can compete with the crops for nutrients in soil to influence the growth of the crops;
the large crabgrass is one of weeds which harm farmlands and orchards, and the large amount of weeds in the prior art is cleared by adopting a herbicide;
herbicides are agents which can kill weeds completely or selectively, and are also called herbicides, and are used for killing or inhibiting plant growth.
Disclosure of Invention
The invention aims to solve the problems in the background technology, and provides a preparation method of a high-efficiency environment-friendly biological herbicide.
In order to achieve the purpose, the invention adopts the following technical scheme:
a preparation method of a high-efficiency environment-friendly biological herbicide comprises the following steps:
s1: preparing a herbicide A; wherein the herbicide agent A comprises: atrazine, anthrax bacteria, brassinolide, fluazifop-p-butyl, Fusarium pachydenum, and wetting agent;
s2: preparing a herbicide B; wherein the herbicide agent B comprises: sodium diquat, compound sodium nitrophenolate and paecilomyces lilacinus fungicide;
s3: preparing a herbicide C; wherein the herbicide agent C comprises: calcium oxide, kudzu and biomass carbon.
Preferably, the herbicide A comprises the following raw materials in parts by weight: 10-15 parts of atrazine; 10-15 parts of anthrax bacteria; 15-25 parts of brassinolide; 8-17 parts of fluazifop-p-butyl; 20-30 parts of Fusarium pachytrum; 5-15 parts of a wetting agent; the herbicide B comprises the following raw materials in parts by weight: 10-15 parts of sodium diurethane; 5-10 parts of compound sodium nitrophenolate; 5-20 parts of paecilomyces lilacinus microbial inoculum; the herbicide C comprises the following raw materials in parts by weight: 15-35 parts of calcium oxide; 15-20 parts of kudzu vine; 20-30 parts of biomass carbon.
Preferably, step S1 specifically includes the following steps:
a. culturing anthrax and Fusarium pachytrum and harvesting spores, washing and resuspending spores with sterile double distilled water;
b. mixing anthrax, Fusarium pachysteinum, atrazine, brassinolide and fluazifop-p-butyl in a three-dimensional mixer, and adding sterile distilled water for mixing for 1 h;
c. and (c) adding a wetting agent in the mixing process of the step (b) to prepare the herbicide A.
Preferably, the wetting agent is soy lecithin.
Preferably, step S2 specifically includes the following steps:
d. grinding the solid sodium diurethane into powder in a grinding machine, and sieving the ground sodium diurethane powder through a sieve with 200 meshes and 250 meshes for later use;
e. culturing the paecilomyces lilacinus by a culture medium, and sequentially putting the cultured paecilomyces lilacinus, the sodium diurethane powder, the compound sodium nitrophenolate and the sterile water into a three-dimensional mixer for mixing operation, wherein the mixing time is 0.5 h;
f. and d, adding egg yolks in the mixing process of the step d to prepare the herbicide B.
Preferably, step S3 specifically includes the following steps:
g. preparing calcium oxide powder;
h. preparing kudzu powder:
i. adding the calcium oxide powder and the kudzu powder into the biomass carbon for mixing operation to obtain the herbicide C.
Preferably, the preparation of the calcium oxide powder in the step g comprises the following steps:
crushing the selected limestone by a crusher, and screening the crushed limestone by a sieve with 200 meshes through a 150-mesh screen for later use;
placing the screened limestone into a rotary kiln furnace for calcination, collecting the calcined lime, namely calcium oxide, and screening the collected calcium oxide powder through a screen with 200-mesh and 250-mesh meshes for later use;
preferably, the preparation of kudzu powder in step h comprises the following steps:
thirdly, placing the picked kudzu under the sun for insolation, and then placing the kudzu into a dryer for drying;
fourthly, putting the dried kudzu into a crusher for crushing, screening by a 200-mesh and 250-mesh screen for later use.
Preferably, the calcination time of the limestone in the step (II) is 1.5-2h, and the calcination temperature is 700-1100 ℃.
Preferably, the crusher is a hammer crusher.
Compared with the prior art, the invention provides a preparation method of a high-efficiency environment-friendly biological herbicide, which has the following beneficial effects:
1. the preparation method of the high-efficiency environment-friendly biological herbicide comprises the following steps:
firstly, preparing a herbicide A; wherein the herbicide agent A comprises: the herbicide composition comprises 12.5g of atrazine, 12.5g of anthrax, 20g of brassinolide, 12.5g of fluazifop-p-butyl, 25g of fusarium pachytron and 10g of wetting agent, wherein the lubricating agent specifically adopts soybean lecithin, so that the lubricating effect can be achieved, and meanwhile, the nutrient can be supplemented to soil when the herbicide A is used;
during preparation, firstly culturing the anthrax and the fusarium pachytron and harvesting spores, and washing and resuspending the spores by using sterile double distilled water;
placing the anthrax, the fusarium pachyspora, the atrazine, the brassinolide and the fluazifop-p-butyl into a three-dimensional mixer for mixing, adding sterile distilled water for mixing, and simultaneously adding the soybean lecithin into the three-dimensional mixer for mixing for 1 h;
then preparing a herbicide B; wherein the herbicide agent B comprises: 12.5g of sodium diquat, 7.5g of compound sodium nitrophenolate and 12.5g of paecilomyces lilacinus;
during preparation, the solid sodium diurethane is put into a grinding machine to be ground into powder, and the ground sodium diurethane powder is screened by a 225-mesh screen for later use;
culturing the paecilomyces lilacinus by using a culture medium, sequentially putting the cultured paecilomyces lilacinus, the sodium diurethane powder, the compound sodium nitrophenolate and the sterile water into a three-dimensional mixer for mixing operation, adding egg yolk in the mixing process, and taking the egg yolk as a biological matrix of the paecilomyces lilacinus for mixing for 0.5 h;
finally, preparing a herbicide C; wherein the herbicide agent C comprises: 22.5g of calcium oxide, 17.5g of kudzu and 25g of biomass carbon.
During preparation, the selected limestone is crushed by a hammer crusher, and the crushed limestone is screened by a 175-mesh screen for later use;
placing the screened limestone into a rotary kiln furnace for calcination, wherein the calcination time is 1.5h, the calcination temperature is 900 ℃, collecting lime generated by calcination, namely calcium oxide, and screening the collected calcium oxide powder by a 225-mesh screen;
placing the picked kudzu vine under the sun for insolation, and then placing the kudzu vine into a dryer for drying;
putting the dried kudzu into a hammer crusher for crushing, and screening by using a 225-mesh screen;
by adding atrazine, anthrax, brassinolide, fluazifop-p-butyl, Fusarium pachytron and a wetting agent into the herbicide A, the environment of soil can be improved, the growth of weed seeds can be inhibited, the atrazine is a systemic selective pre-emergence and post-emergence closed herbicide, the growth of large crabgrass in soil can be well inhibited, meanwhile, barnyard grass, green bristlegrass or other perennial weeds can also be inhibited, but after the atrazine is added, the atrazine has certain phytotoxicity, the growth of crops is influenced, and the phytotoxicity of the atrazine in the environment with high humidity is larger;
by adding the sodium diquat, the compound sodium nitrophenolate and the paecilomyces lilacinus into the herbicide B, the growth of crops can be promoted, the activity of plant cells can be improved, the sodium diquat can treat fungi such as pythium aphanidermatum and smut bacteria in soil to protect the soil, and meanwhile, a certain amount of the sodium diquat can remove the harm of atrazine added into the herbicide A to the residual soil;
by adding calcium oxide, kudzu and biomass carbon into the herbicide C, the kudzu contains a large amount of active materials mainly containing alkali, the active materials can strongly inhibit the germination of grass seeds, the calcium oxide can react with water in soil to generate calcium hydroxide and release heat, so that the soil environment is dry, the phytotoxicity of atrazine is reduced, the calcium hydroxide is alkaline, and the sodium diuron can be decomposed under alkaline conditions or heated conditions;
the atrazine can be added into the herbicide to effectively inhibit the growth and the germination of the crabgrass seeds, the phytotoxicity of the atrazine on crops can be reduced and the growth of the crops can be promoted by adding the sodium diurethane into the herbicide, and the phytotoxicity of the atrazine can be reduced, the sodium diurethane can be decomposed and the toxicity of the soil can be reduced by adding the calcium oxide into the herbicide.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments.
In the description of the present invention, it is to be understood that the terms "upper", "lower", "front", "rear", "left", "right", "top", "bottom", "inner", "outer", and the like, indicate orientations and positional relationships for convenience in describing the present invention and simplifying the description, but do not indicate or imply that the referenced devices or elements must have a particular orientation, be constructed and operated in a particular orientation, and thus, should not be construed as limiting the present invention.
Example 1:
a preparation method of a high-efficiency environment-friendly biological herbicide comprises the following steps:
firstly, preparing a herbicide A; wherein the herbicide agent A comprises: the herbicide composition comprises 12.5g of atrazine, 12.5g of anthrax, 20g of brassinolide, 12.5g of fluazifop-p-butyl, 25g of fusarium pachytron and 10g of wetting agent, wherein the lubricating agent specifically adopts soybean lecithin, so that the lubricating effect can be achieved, and meanwhile, the nutrient can be supplemented to soil when the herbicide A is used;
during preparation, firstly culturing the anthrax and the fusarium pachytron and harvesting spores, and washing and resuspending the spores by using sterile double distilled water;
placing the anthrax, the fusarium pachyspora, the atrazine, the brassinolide and the fluazifop-p-butyl into a three-dimensional mixer for mixing, adding sterile distilled water for mixing, and simultaneously adding the soybean lecithin into the three-dimensional mixer for mixing for 1 h;
then preparing a herbicide B; wherein the herbicide agent B comprises: 12.5g of sodium diquat, 7.5g of compound sodium nitrophenolate and 12.5g of paecilomyces lilacinus;
during preparation, the solid sodium diurethane is put into a grinding machine to be ground into powder, and the ground sodium diurethane powder is screened by a 225-mesh screen for later use;
culturing the paecilomyces lilacinus by using a culture medium, sequentially putting the cultured paecilomyces lilacinus, the sodium diurethane powder, the compound sodium nitrophenolate and the sterile water into a three-dimensional mixer for mixing operation, adding egg yolk in the mixing process, and taking the egg yolk as a biological matrix of the paecilomyces lilacinus for mixing for 0.5 h;
finally, preparing a herbicide C; wherein the herbicide agent C comprises: 22.5g of calcium oxide, 17.5g of kudzu and 25g of biomass carbon.
During preparation, the selected limestone is crushed by a hammer crusher, and the crushed limestone is screened by a 175-mesh screen for later use;
placing the screened limestone into a rotary kiln furnace for calcination, wherein the calcination time is 1.5h, the calcination temperature is 900 ℃, collecting lime generated by calcination, namely calcium oxide, and screening the collected calcium oxide powder by a 225-mesh screen;
placing the picked kudzu vine under the sun for insolation, and then placing the kudzu vine into a dryer for drying;
putting the dried kudzu into a hammer crusher for crushing, and screening by using a 225-mesh screen;
by adding atrazine, anthrax, brassinolide, fluazifop-p-butyl, Fusarium pachytron and a wetting agent into the herbicide A, the environment of soil can be improved, the growth of weed seeds can be inhibited, the atrazine is a systemic selective pre-emergence and post-emergence closed herbicide, the growth of large crabgrass in soil can be well inhibited, meanwhile, barnyard grass, green bristlegrass or other perennial weeds can also be inhibited, but after the atrazine is added, the atrazine has certain phytotoxicity, the growth of crops is influenced, and the phytotoxicity of the atrazine in the environment with high humidity is larger;
by adding the sodium diquat, the compound sodium nitrophenolate and the paecilomyces lilacinus into the herbicide B, the growth of crops can be promoted, the activity of plant cells can be improved, the sodium diquat can treat fungi such as pythium aphanidermatum and smut bacteria in soil to protect the soil, and meanwhile, a certain amount of the sodium diquat can remove the harm of atrazine added into the herbicide A to the residual soil;
by adding calcium oxide, kudzu and biomass carbon into the herbicide C, the kudzu contains a large amount of active materials mainly containing alkali, the active materials can strongly inhibit the germination of grass seeds, the calcium oxide can react with water in soil to generate calcium hydroxide and release heat, so that the soil environment is dry, the phytotoxicity of atrazine is reduced, the calcium hydroxide is alkaline, and the sodium diuron can be decomposed under alkaline conditions or heated conditions;
when the herbicide a, the herbicide B, and the herbicide C are used:
selecting 30 square meters of soil to be planted, adding 500 grains of crabgrass seeds, mixing the soil to uniformly mix the soil, adding a herbicide A, and spraying the herbicide A in a spray manner;
observing for 1-2 weeks after spraying, adding herbicide B, spraying herbicide B in a spray mode, and covering with an opaque film;
after 1-2 weeks, the opaque film is removed, and the herbicide C is uniformly sprayed in the soil;
planting 1000 soybean grains at equal intervals after 1-2 weeks, observing germination rate and growth vigor of soybean, adding water into small amount of soil, filtering, and observing color of the filtered water solution.
Example 2:
substantially the same as in example 1, except that atrazine was not added in the process of preparing the herbicide a in a transparent state;
when the herbicide a, the herbicide B, and the herbicide C are used:
selecting 30 square meters of soil to be planted, adding 500 grains of crabgrass seeds, mixing the soil to uniformly mix the soil, adding a herbicide A, and spraying the herbicide A in a spray manner;
observing for 1-2 weeks after spraying, adding herbicide B, spraying herbicide B in a spray mode, and covering with an opaque film;
after 1-2 weeks, the opaque film is removed, and the herbicide C is uniformly sprayed in the soil;
planting 1000 soybean grains at equal intervals after 1-2 weeks, observing germination rate and growth vigor of soybean, adding water into small amount of soil, filtering, and observing color of the filtered water solution.
Example 3:
substantially the same as in example 1, except that no sodium diuride was added during the preparation of the herbicide B;
when the herbicide a, the herbicide B, and the herbicide C are used:
selecting 30 square meters of soil to be planted, adding 500 grains of crabgrass seeds, mixing the soil to uniformly mix the soil, adding a herbicide A, and spraying the herbicide A in a spray manner;
observing for 1-2 weeks after spraying, adding herbicide B, spraying herbicide B in a spray mode, and covering with an opaque film;
after 1-2 weeks, the opaque film is removed, and the herbicide C is uniformly sprayed in the soil;
planting 1000 soybean grains at equal intervals after 1-2 weeks, observing germination rate and growth vigor of soybean, adding water into small amount of soil, filtering, and observing color of the filtered water solution.
Example 4:
substantially the same as in example 1, except that in the process of preparing the herbicide agent C, calcium oxide was not added;
when the herbicide a, the herbicide B, and the herbicide C are used:
selecting 30 square meters of soil to be planted, adding 500 grains of crabgrass seeds, mixing the soil to uniformly mix the soil, adding a herbicide A, and spraying the herbicide A in a spray manner;
observing for 1-2 weeks after spraying, adding herbicide B, spraying herbicide B in a spray mode, and covering with an opaque film;
after 1-2 weeks, the opaque film is removed, and the herbicide C is uniformly sprayed in the soil;
planting 1000 soybeans at equal intervals after 1-2 weeks, observing the germination rate and growth vigor of the soybeans and judging the germination rate of the crabgrass seeds, adding water into a small amount of soil, filtering, and observing the color of the filtered water solution.
Table 1: growth result table for soybean and large crabgrass
As is clear from examples 1 to 4 and table 1, the present invention can effectively inhibit the growth and germination of crabgrass seeds by adding atrazine to the herbicide, can reduce the phytotoxicity of atrazine to crops and promote the growth of crops by adding sodium diuron to the herbicide, and can reduce the phytotoxicity of atrazine and decompose sodium diuron to reduce the toxicity of soil by adding calcium oxide to the herbicide.
The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art should be considered to be within the technical scope of the present invention, and the technical solutions and the inventive concepts thereof according to the present invention should be equivalent or changed within the scope of the present invention.
Claims (10)
1. A preparation method of a high-efficiency environment-friendly biological herbicide is characterized by comprising the following steps:
s1: preparing a herbicide A;
wherein the herbicide agent A comprises: atrazine, anthrax bacteria, brassinolide, fluazifop-p-butyl, Fusarium pachydenum, and wetting agent;
s2: preparing a herbicide B;
wherein the herbicide agent B comprises: sodium diquat, compound sodium nitrophenolate and paecilomyces lilacinus fungicide;
s3: preparing a herbicide C;
wherein the herbicide agent C comprises: calcium oxide, kudzu and biomass carbon.
2. The preparation method of the efficient environment-friendly biological herbicide as claimed in claim 1, wherein the herbicide A comprises the following raw materials in parts:
10-15 parts of atrazine;
10-15 parts of anthrax bacteria;
15-25 parts of brassinolide;
8-17 parts of fluazifop-p-butyl;
20-30 parts of Fusarium pachytrum;
5-15 parts of a wetting agent;
the herbicide B comprises the following raw materials in parts by weight:
10-15 parts of sodium diurethane;
5-10 parts of compound sodium nitrophenolate;
5-20 parts of paecilomyces lilacinus microbial inoculum;
the herbicide C comprises the following raw materials in parts by weight:
15-35 parts of calcium oxide;
15-20 parts of kudzu vine;
20-30 parts of biomass carbon.
3. The method for preparing the efficient environment-friendly biological herbicide as claimed in claim 2, wherein the step S1 specifically comprises the following steps:
a. culturing anthrax and Fusarium pachytrum and harvesting spores, washing and resuspending spores with sterile double distilled water;
b. mixing anthrax, Fusarium pachysteinum, atrazine, brassinolide and fluazifop-p-butyl in a three-dimensional mixer, and adding sterile distilled water for mixing for 1 h;
c. and (c) adding a wetting agent in the mixing process of the step (b) to prepare the herbicide A.
4. The method for preparing a highly potent and environment-friendly bioherbicide as claimed in claim 3, wherein said wetting agent is soybean lecithin.
5. The method for preparing the efficient environment-friendly biological herbicide as claimed in claim 2, wherein the step S2 specifically comprises the following steps:
d. grinding the solid sodium diurethane into powder in a grinding machine, and sieving the ground sodium diurethane powder through a sieve with 200 meshes and 250 meshes for later use;
e. culturing the paecilomyces lilacinus by a culture medium, and sequentially putting the cultured paecilomyces lilacinus, the sodium diurethane powder, the compound sodium nitrophenolate and the sterile water into a three-dimensional mixer for mixing operation, wherein the mixing time is 0.5 h;
f. and d, adding egg yolks in the mixing process of the step d to prepare the herbicide B.
6. The method for preparing the efficient environment-friendly biological herbicide as claimed in claim 2, wherein the step S3 specifically comprises the following steps:
g. preparing calcium oxide powder;
h. preparing kudzu powder:
i. adding the calcium oxide powder and the kudzu powder into the biomass carbon for mixing operation to obtain the herbicide C.
7. The method for preparing efficient and environment-friendly biological herbicide as claimed in claim 6, wherein the preparation of calcium oxide powder in step g comprises the following steps:
crushing the selected limestone by a crusher, and screening the crushed limestone by a sieve with 200 meshes through a 150-mesh screen for later use;
and secondly, placing the screened limestone into a rotary kiln furnace for calcination, collecting the calcined lime, namely calcium oxide, and screening the collected calcium oxide powder through a screen with 200 meshes and 250 meshes for later use.
8. The method for preparing efficient environment-friendly biological herbicide as claimed in claim 6, wherein the step h of preparing kudzu powder comprises the following steps:
thirdly, placing the picked kudzu under the sun for insolation, and then placing the kudzu into a dryer for drying;
fourthly, putting the dried kudzu into a crusher for crushing, screening by a 200-mesh and 250-mesh screen for later use.
9. The method as claimed in claim 7, wherein the calcination time of the limestone in step (II) is 1.5-2h, and the calcination temperature is 700-1100 ℃.
10. The method for preparing a highly efficient and environmentally friendly bio-herbicide as claimed in any one of claims 7 to 9, wherein said crusher is a hammer crusher.
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