CN111690735B - 用于检测单基因糖尿病的相关基因的引物组、试剂盒及方法 - Google Patents
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Abstract
本申请公开了一种用于检测单基因糖尿病的致病基因的引物组、试剂盒及方法。通过多重扩增联合二代测序方法以及针对该体系创建的实验和分析方法,在一次PCR反应中,可以对37个基因的外显子以及左右两侧50bp区域进行基因检测、寻找突变位点并对突变位点进行结果注释,从基因的水平上进行疾病分型,从而达到辅助诊断的目的。方法简单易行,快速高效,成本低廉,为单基因糖尿病的临床筛查提供一个可行的简单的筛选方法。
Description
技术领域
本发明涉及对单基因糖尿病的相关基因进行扩增的引物组。
本发明涉及用于对单基因糖尿病的相关基因进行检测的试剂盒。
本发明涉及对单基因糖尿病的相关基因进行检测的方法。
背景技术
单基因糖尿病主要包括新生儿糖尿病、成年起病型青少年糖尿病(MODY)、线粒体糖尿病和综合征性的罕见糖尿病。这类疾病发病率很低,新生儿糖尿病发病率大约为二十万分之一,MODY大约占全部糖尿病人群的1%-3%。由于发病早,症状严重,人口基数大,即使发病率低,患病总数也很高:我国大概有8百万人患有单基因糖尿病,这是一个不容忽视的数字,成为临床诊疗中亟待解决的重要问题。
截止到2018年,已经发现超过30个单基因糖尿病相关致病基因,对应的机制涉及到胰岛β细胞的发育、数量、胰岛素分泌和功能等。在已经发现的不同分子分型的单基因糖尿病中,有几类是有明确的治疗方案,可以使患者摆脱注射胰岛素的痛苦,转向口服药,包括HNF1A、KCNJ11和ABCC8等基因突变导致的糖尿病;甚至可以不用药物治疗,如MODY-GCK,极大地促进了个体化医疗的发展。在已经发现的14个不同的MODY亚型中,GCK、HNF1A和HNF4A亚型在英国MODY人群中分别占到52%,10%和32%。尽管不同的国家主要MODY亚型不同,但是总体而言,这三种MODY亚型大概占到85%-90%的比例。
由于遗传学检测不够普及的原因,大概有80%的单基因糖尿病患者被误诊为1型或2型糖尿病而接受错误的治疗方案,严重影响患者的生活质量和家庭负担。在测序技术快速发展的推动下,目前英美等发达国家已启动糖尿病人群筛查网络来突破单基因糖尿病精准诊断及治疗的瓶颈。2014年,英国国民保健服务系统(NHS)批准了一款针对单基因糖尿病患者的一整套解决方案。其中包括临床表型MODY-APP筛查、基因检测、测序结果分析、验证、解读以及咨询等。随着新基因的发现,该panel也不断更新,由原来的14个基因扩增到了32个基因的范围。为罕见糖尿病患者提供一个简单明确的检测方案。但是,即使在目前测序成本逐年下降的情况下,该检测费用仍然持高不下,成本在1000-5000元不等。这也严重限制了临床推广应用。
我国目前在单基因糖尿病诊治领域的现状不容乐观。由于人口基数大,单基因糖尿病患者数目庞大,但是基因检测的分子分型诊断落后,急切需要规范的罕见糖尿病诊疗的流程,结合临床实践和实验室检查,建立专业快速的分子遗传学诊断路线,为临床诊断和治疗服务。
发明内容
根据本发明的一个方面,提供一种引物组合。所述引物组合中,任一引物的扩增片段选自单基因糖尿病致病基因中至少一个基因中的至少一段序列。
优选地,所述单基因糖尿病致病基因包括LMNA、PPP1R15B、KLF11、DCAF17、NEUROD1、PPARG、APPL1、WFS1、TRMT10A、CISD2、PIK3R1、ZFP57、RFX6、ZBTB2、GCK、PAX4、BLK、GATA4、CEL、PCBD1、SLC29A3、INS、ABCC8、KCNJ11、PAX6、HNF1A、PDX1、DNAJC3、PLIN1、HNF1B、GATA6、INSR、DYRK1B、AKT2、POLD1、HNF4A和MTTL1。
优选地,所述扩增片段长度在150bp至299bp之间。
可选地,所述引物组合选自针对所述单基因糖尿病致病基因设计的序列依次为SEQ ID No.1-1502的引物对中的至少一对引物,或与SEQ ID No.1-1502所示的核苷酸序列具有至少80%同一性的核苷酸序列的引物。
优选地,所述引物组合中,正向引物序列选自针对所述单基因糖尿病致病基因设计的序列依次为SEQ ID No.1-751;反向引物序列选自针对所述单基因糖尿病致病基因设计的序列依次为SEQ ID No.752-1502。
优选地,所述引物组合由751对引物组成;所述751对引物,为序列SEQ ID No.1-751的正向引物,依次与序列为SEQ ID No.752-1502的反向引物配对,形成的组合。
所述SEQ ID No.1-751的正向引物,依次与序列为SEQ ID No.752-1502的反向引物配对,信息如下表1所示。
表1
根据本发明的又一方面,提供一种试剂盒,其包含所述引物组合。
根据本发明的又一方面,提供一种在生物样品中检测单基因糖尿病致病基因的存在的方法。该通过多重扩增联合二代测序方法以及针对该体系创建的实验和分析方法,在一次PCR反应中,可以对37个基因的所有外显子以及每个外显子左右两侧50bp区域进行基因检测、寻找突变位点并对突变位点进行结果注释,从基因的水平上进行疾病分型,从而达到辅助诊断的目的。方法简单易行,快速高效,成本低廉,为单基因糖尿病的临床筛查提供一个可行的简单的筛选方法。
所述在生物样品中检测单基因糖尿病致病基因的存在的方法,包括以下的步骤:
i)在包括多重PCR测定中将所述生物样品或从中分离的核酸与引物组合相混合,得到反应混合物;其中所述引物组合选自上述任意引物组合;
ii)利用从步骤i)中获得的反应混合物进行聚合酶链式反应,若所述生物样品中存在所述序列,从而特异性扩增单基因糖尿病致病基因的扩增片段,得到反映产物;
iii)在所述反应产物中检测片段序列的存在,其中任何所述单基因糖尿病致病基因的扩增片段的存在,表示所述样品中相应的单基因糖尿病致病基因的存在。
可选地,所述步骤(ii)中聚合酶链式反应的条件为:
96-98℃预变性3-5分钟,1个循环;
96℃变性30秒,58-62℃退火延伸3-6分钟,共15~19个循环;
72℃彻底延伸4分钟,1个循环。
优选地,所述步骤(ii)中聚合酶链式反应的条件为:96℃预变性3分钟,1个循环;96℃变性30秒,60℃退火延伸4分钟,共15~19个循环;72℃彻底延伸4分钟,1个循环。
可选地,所述在生物样品中检测单基因糖尿病致病基因的存在的方法具备下述条件(1)至(37)中的至少一种:
(1):所述序列为SEQ ID No.33-68,410-443的正向引物或序列为SEQ ID No.784-819,1161-1194的反向引物的扩增片段表明ABCC8单基因糖尿病致病基因的存在;
(2):所述序列为SEQ ID No.166-175,544-553的正向引物或序列为SEQ IDNo.917-926,1295-1304的反向引物的扩增片段表明AKT2单基因糖尿病致病基因的存在;
(3):所述序列为SEQ ID No.240-256,617-634的正向引物或序列为SEQ IDNo.991-1007,1368-1385的反向引物的扩增片段表明APPL1单基因糖尿病致病基因的存在;
(4):所述序列为SEQ ID No.345-356,722-733的正向引物或序列为SEQ IDNo.1096-1107,1473-1484的反向引物的扩增片段表明BLK单基因糖尿病致病基因的存在;
(5):所述序列为SEQ ID No.365-376,741-751的正向引物或序列为SEQ IDNo.1116-1127,1492-1502的反向引物的扩增片段表明CEL单基因糖尿病致病基因的存在;
(6):所述序列为SEQ ID No.275-280,652-654的正向引物或序列为SEQ IDNo.1026-1031,1403-1405的反向引物的扩增片段表明CISD2单基因糖尿病致病基因的存在;
(7):所述序列为SEQ ID No.205-216,582-593的正向引物或序列为SEQ IDNo.956-967,1333-1344的反向引物的扩增片段表明DCAF17单基因糖尿病致病基因的存在;
(8):所述序列为SEQ ID No.95-105,471-482的正向引物或序列为SEQ IDNo.846-856,1222-1233的反向引物的扩增片段表明DNAJC3单基因糖尿病致病基因的存在;
(9):所述序列为SEQ ID No.156-165,535-543的正向引物或序列为SEQ IDNo.907-916,1286-1294的反向引物的扩增片段表明DYRK1B单基因糖尿病致病基因的存在;
(10):所述序列为SEQ ID No.357-364,734-740的正向引物或序列为SEQ IDNo.1108-1115,1485-1491的反向引物的扩增片段表明GATA4单基因糖尿病致病基因的存在;
(11):所述序列为SEQ ID No.123-130,499-509的正向引物或序列为SEQ IDNo.874-881,1250-1260的反向引物的扩增片段表明GATA6单基因糖尿病致病基因的存在;
(12):所述序列为SEQ ID No.326-336,701-711的正向引物或序列为SEQ IDNo.1077-1087,1452-1462的反向引物的扩增片段表明GCK单基因糖尿病致病基因的存在;
(13):所述序列为SEQ ID No.80-90,453-463的正向引物或序列为SEQ IDNo.831-841,1204-1214的反向引物的扩增片段表明HNF1A单基因糖尿病致病基因的存在;
(14):所述序列为SEQ ID No.114-122,490-498的正向引物或序列为SEQ IDNo.865-873,1241-1249的反向引物的扩增片段表明HNF1B单基因糖尿病致病基因的存在;
(15):所述序列为SEQ ID No.220-232,597-608的正向引物或序列为SEQ IDNo.971-983,1348-1359的反向引物的扩增片段表明HNF4A单基因糖尿病致病基因的存在;
(16):所述序列为SEQ ID No.28、29、404、405的正向引物或序列为SEQ IDNo.779、780、1155、1156的反向引物的扩增片段表明INS单基因糖尿病致病基因的存在;
(17):所述序列为SEQ ID No.131-155,510-534的正向引物或序列为SEQ IDNo.882-906,1261-1285的反向引物的扩增片段表明INSR单基因糖尿病致病基因的存在;
(18):所述序列为SEQ ID No.30-32,406-409的正向引物或序列为SEQ IDNo.781-783,1157-1160的反向引物的扩增片段表明KCNJ11单基因糖尿病致病基因的存在;
(19):所述序列为SEQ ID No.198-204,576-581的正向引物或序列为SEQ IDNo.949-955,1327-1332的反向引物的扩增片段表明KLF11单基因糖尿病致病基因的存在;
(20):所述序列为SEQ ID No.1-12,378-389的正向引物或序列为SEQ ID No.752-763,1129-1140的反向引物的扩增片段表明LMNA单基因糖尿病致病基因的存在;
(21):所述序列为SEQ ID No.377的正向引物或序列为SEQ ID No.1128的反向引物的扩增片段表明MTTL单基因糖尿病致病基因的存在;
(22):所述序列为SEQ ID No.217-219,594-596的正向引物或序列为SEQ IDNo.968-970,1345-1347的反向引物的扩增片段表明NEUROD1单基因糖尿病致病基因的存在;
(23):所述序列为SEQ ID No.337-344,712-721的正向引物或序列为SEQ IDNo.1088-1095,1463-1472的反向引物的扩增片段表明PAX4单基因糖尿病致病基因的存在;
(24):所述序列为SEQ ID No.69-79,444-452的正向引物或序列为SEQ IDNo.820-830,1195-1203的反向引物的扩增片段表明PAX6单基因糖尿病致病基因的存在;
(25):所述序列为SEQ ID No.19-21,396-397的正向引物或序列为SEQ IDNo.770-772,1147-1148的反向引物的扩增片段表明PCBD1单基因糖尿病致病基因的存在;
(26):所述序列为SEQ ID No.91-94,464-470的正向引物或序列为SEQ IDNo.842-845,1215-1221的反向引物的扩增片段表明PDX1单基因糖尿病致病基因的存在;
(27):所述序列为SEQ ID No.281-295,655-670的正向引物或序列为SEQ IDNo.1032-1046,1406-1421的反向引物的扩增片段表明PIK3R1单基因糖尿病致病基因的存在;
(28):所述序列为SEQ ID No.106-113,483-489的正向引物或序列为SEQ IDNo.857-864,1234-1240的反向引物的扩增片段表明PLIN1单基因糖尿病致病基因的存在;
(29):所述序列为SEQ ID No.176-197,554-575的正向引物或序列为SEQ IDNo.927-948,1305-1326的反向引物的扩增片段表明POLD1单基因糖尿病致病基因的存在;
(30):所述序列为SEQ ID No.233-239,609-616的正向引物或序列为SEQ IDNo.984-990,1360-1367的反向引物的扩增片段表明PPARG单基因糖尿病致病基因的存在;
(31)所述序列为SEQ ID No.13-18,390-395的正向引物或序列为SEQ ID No.764-769,1141-1146的反向引物的扩增片段表明PPP1R15B单基因糖尿病致病基因的存在;
(32):所述序列为SEQ ID No.301-320,677-696的正向引物或序列为SEQ IDNo.1052-1071,1428-1447的反向引物的扩增片段表明RFX6单基因糖尿病致病基因的存在;
(33):所述序列为SEQ ID No.22-27,398-403的正向引物或序列为SEQ IDNo.773-778,1149-1154的反向引物的扩增片段表明SLC29A3单基因糖尿病致病基因的存在;
(34):所述序列为SEQ ID No.268-274,645-651的正向引物或序列为SEQ IDNo.1019-1025,1396-1402的反向引物的扩增片段表明TRMT10A单基因糖尿病致病基因的存在;
(35):所述序列为SEQ ID No.257-267,635-644的正向引物或序列为SEQ IDNo.1008-1018,1386-1395的反向引物的扩增片段表明WFS1单基因糖尿病致病基因的存在;
(36):所述序列为SEQ ID No.321-325,697-700的正向引物或序列为SEQ IDNo.1072-1076,1448-1451的反向引物的扩增片段表明ZBTB2单基因糖尿病致病基因的存在;
(37):所述序列为SEQ ID No.296-300,671-676的正向引物或序列为SEQ IDNo.1047-1051,1422-1427的反向引物的扩增片段表明ZFP57单基因糖尿病致病基因的存在。
优选地,所述在生物样品中检测单基因糖尿病致病基因的存在的方法具备所述条件(1)至(37)。
本发明的有益效果包括但不限于:
(1)本发明提供了一种用来辅助单基因糖尿病的临床诊断且价格低廉的常规基因检测产品。解决了目前我国单基因糖尿病患者数目庞大,但是基因检测的分子分型诊断落后,急切需要规范的罕见糖尿病诊疗的流程,结合临床实践和实验室检查,建立专业快速的分子遗传学诊断路线,为临床诊断和治疗服务的难题。
(2)本发明提供的试剂盒,通过多重扩增联合二代测序方法以及针对该体系创建的实验和分析方法,在一次PCR反应中,可以对37个基因的外显子以及左右两侧50bp区域进行基因检测、寻找突变位点并对突变位点进行结果注释,从基因的水平上进行疾病分型,从而达到辅助诊断的目的。
(3)本发明提供的检测方法简单易行,快速高效,成本低廉,为单基因糖尿病的临床筛查提供一个可行的简单的筛选方法。
(4)本发明提供的检测方法,采用特异性的多重PCR反应扩增后进行高通量测序,将待检测基因富集,去除非目标区域的干扰,提高单基因糖尿病相关基因分型检测的准确性。
(5)本发明提供的方案,可针对有明确治疗方案的单基因糖尿病,给出治疗建议。
具体实施方式
下面结合实施例详述本申请、但本申请并不局限于这些实施例。
如无特殊说明,下述实施例中所使用的实验方法,均为常规方法。
如无特殊说明,下述实施例中所用原料和试剂均来自商业购买、未经处理直接使用、所用仪器设备采用厂家推荐的方案和参数。
实施例1
本实施例用于说明使用本发明所述引物组合,针对单基因糖尿病致病基因检测的方法,具体步骤如下:
步骤A:样品
对从医院或其他研究机构获得的血样、唾液或其他组织样本,利用DNA提取试剂盒提取全基因组DNA,并用核酸定量仪对提取的DNA进行浓度测定,DNA浓度为浓度50ng/ul,总量不低于200ng。
针对以上样本,配置PCR反应体系,对LMNA,PPP1R15B,KLF11,DCAF17,NEUROD1,PPARG,APPL1,WFS1,TRMT10A,CISD2,PIK3R1,ZFP57,RFX6,ZBTB2,GCK,PAX4,BLK,GATA4,CEL,PCBD1,SLC29A3,INS,ABCC8,KCNJ11,PAX6,HNF1A,PDX1,DNAJC3,PLIN1,HNF1B,GATA6,INSR,DYRK1B,AKT2,POLD1,HNF4A和MTTL1。进行扩增,分别以MJ1909CL37 Panel Mix A、MJ1909CL37 Panel Mix B为引物,引物序列如表2所示。
表2
MJ1909CL37 Panel Mix A | MJ1909CL37 Panel Mix B |
SEQ ID No.1-377和SEQ ID No.752-1128 | SEQ ID No.378-751和SEQ ID No.1129-1502 |
PCR反应体系:25μL
①第一步PCR-MIX:
试剂名称 | 体积/孔 |
HGC Enzyme | 10μL |
Milli-Q Water | 7μL |
MJ1909CL37Panel Mix A(MJ1909CL37 Panel Mix B) | 6μL |
②第二步PCR-MIX:
试剂名称 | 体积/孔 |
Milli-Q Water | 10μL |
HGC Enzyme | 10μL |
Barcode-MIX | 5μL |
Barcode-MIX配置比例为1μL I5:1μL I7:3μL Milli-Q Water。
PCR反应条件:
①第一步PCR程序:
②第二步PCR程序:
步骤B:建库过程
B1试剂配置:
1)分别以MJ1909CL37 Panel Mix A、MJ1909CL37 Panel Mix B为引物,按照第一步PCR反应体系进行第一步PCR扩增试剂配置,先将所需试剂加入到一个新的离心管中,试剂配置完成后混匀离心按照每孔23μL分装到96孔PCR版中,置于4℃保存。
2)按照第二步PCR反应体系进行第二步PCR扩增试剂配置,先将Milli-Q Water与HGC Enzyme按照实际用量加入到离心管中,混匀离心后按照每孔20μL分装到96孔PCR版中,再加入Barcode_Mixture每孔5μL,混匀离心后置于4℃保存。
B2:加样:
按照预先设定好的加样位置将DNA与阳性对照分别加入到A,B两种第一步PCR-MIX中,DNA与阳性对照加样量均为2μL。
加样完成后贴上PCR专用膜,混匀离心,运行第一步PCR程序。
B3:第一步纯化
1)第一步PCR扩增结束后,得到A、B两种扩增产物,按照A取14μL,B取11μL,混成一版新的25μLPCR反应液,继续之后的纯化步骤。
2)向混好的PCR反应液中加入27.5μL磁珠,充分混匀,室温静置5min。
3)用磁力架吸附磁珠2-5min,直至溶液澄清为止。用移液器小心去除上清,保留磁珠。
4)加入40μL BW10洗液,充分混匀,室温静置5min,用磁力架吸附磁珠2-5min,直至溶液澄清为止。用移液器小心去除上清,保留磁珠。
5)加入110μL 80%乙醇,用磁力架反复在不同的两面来回吸附磁珠以充分悬浮磁珠以便洗涤。
6)用磁力架吸附磁珠30s,直至溶液澄清为止。用移液器小心去除上清,避免吸到磁珠。
7)室温放置8min,直至乙醇完全挥发。
8)待乙醇完全挥发后,将配置好的第二步PCR-MIX加入到上述含磁珠的96孔PCR版中,贴上PCR专用膜,混匀离心后,运行第二步PCR程序。
B4:第二步纯化
1)第二步PCR扩增结束后,向PCR反应液中加入20μL磁珠,充分混匀,室温静置5min。
2)用磁力架吸附磁珠2-5min,直至溶液澄清为止。用移液器小心去除上清,保留磁珠。
3)加入40μL BW07洗液,充分混匀,室温静置5min,用磁力架吸附磁珠2-5min,直至溶液澄清为止。用移液器小心去除上清,保留磁珠。
4)加入110μL 80%乙醇,用磁力架反复在不同的两面来回吸附磁珠以充分悬浮磁珠以便洗涤。
5)用磁力架吸附磁珠30s,直至溶液澄清为止。用移液器小心去除上清,避免吸到磁珠。
6)室温放置8min,直至乙醇完全挥发。
7)待乙醇完全挥发后,加入21μL TE到含有磁珠的上述96孔PCR版中,混匀离心静置2min后,在磁力架上吸附磁珠2min,直至溶液澄清为止。
8)取一个新的96孔PCR版,用移液器小心吸取上清至其中,避免吸到磁珠。共吸取18μL洗脱液,用10μL排枪吸取两次即可。
B5:文库质检
1)配置1%琼脂糖凝胶电泳,取2μL文库与2μL Loading Buffer混匀后,进行琼脂糖凝胶电泳,电泳条件为170V,200mA,20min。文库电泳时,选择TransPlus II DNAMarker作为标准品。
2)电泳完成后,制作胶图,书写文库质检报告。
B6:文库pooling
Barcode相同的两个文库不能混在一个文库里。该类文库pooling有两类情况,分为胶图条带亮度均一与不均一两种。
条带均一:终文库版中每孔取3μL文库进行文库pooling。
条带不均一;按照胶图亮度抽测文库浓度,预测其余浓度,将每孔浓度稀释至大约2ng/μL后进行文库pooling。2.3.7上机测序。
步骤C:二代测序数据分析
(1)去引物
(2)利用工具Trimmomatic来对测序的reads进行质控,具体运行命令为
java-jar trimmomatic-0.39.jar PE-threads 12data-R1.fastq.gz data-R2.fastq.gz data.R1.paired.fq.gz data.R1.unpaired.fa.gz data.R2.paired.fq.gzdata.R2.unpaired.fq.gz LEADING:3TRAILING:3SLIDINGWINDOW:4:15MINLEN:36ILLUMINACLIP:../../software/Trimmomatic-0.39/adapters/TruSeq3-PE.fa:2:30:10:8:true
(3)用Bwa index对参考序列做index(所选参考序列为hg38)
(4)利用Bwa mem做mapping,并生成sam文件:
bwa mem-M-t 10-R'@RG\tID:2\tSM:55A\tPL:illumina\tLB:LIB2\tPU:unit2'hg38.fasta data.R1.paired.fq.gz data.R2.paired.fq.gz>G-001-bwa.sam 2>G-001-bwa.log
(5)对上一步所得的sam文件进行重新排序,所用工具为ReorderSam.jar:
java-jar ReorderSam.jar REFERENCE=ucsc.hg19.fasta I=G-001-bwa.sam O=G-001-recorder.sam
(6)将排序后的sam文件转化为bam文件,所用命令为:
samtools view-bS G-001-recorder.sam-o G-001-recorder.bam
(7)对生成的bam文件进行排序(主要是对sam文件中同一染色体对应的条目按照坐标顺序从小到大进行排序),所用命令为:
java-jar SortSam.jar INPUT=G-001-recorder.bam OUTPUT=G-001-sorted.bam SORT_ORDER=coordinate
(8)根据已有的SNP和INdel数据库,利用GATK中的BaseRecalibrator和ApplyBQSR工具对原始的碱基质量分数进行校正。具体的命令为:
java-jar gatk-package-4.1.1.0-local.jar BaseRecalibrator-R hg38.fa-IG-001-sorted.bam--known-sites dbsnp_138.hg38.vcf.gz--known-sites Mills_and_1000G_gold_standard.indels.hg38.vcf.gz--known-sites 1000G_phase1.snps.high_confidence.hg38.vcf.gz-O G-001_recal_data.table
java-jar gatk-package-4.1.1.0-local.jar ApplyBQSR-R hg38.fa-I G-001-sorted.bam-bqsr G-001_recal_data.table-O G-001_recal_reads.bam
(9)检测生成的bam文件是否可用
java-jar gatk-package-4.1.1.0-local.jar ValidateSamFile-I G-001_recal_reads.bam
(10)利用GATK4中的HaplotypeCaller生成gvcf文件
(11)利用GATK4中的VariantFiltration对找到的突变位点进行过滤。(过滤的条件为QD<4.0||FS>60.0||MQ<40.0||SOR>3.0||MQRankSum<-12.5||ReadPosRankSum<-8.0)
(12)利用annovar软件对变异位点进行注释。(注释所用的数据库主要有:exac03;gnomad211_genome;1000g2015aug;dbnsfp35c;avsnp150;intervar_20180118;clinvar),数据库具体信息见表3。
表3结果注释数据库
Database | Date |
exac03 | 20151129 |
gnomad211_genome | 20190409 |
1000g2015aug | 20150824 |
dbnsfp35c | 20181023 |
avsnp150 | 20170929 |
intervar_20180118 | 20180325 |
clinvar | 20191231 |
(13)筛选出在1000g、Exac以及Gnomad数据库中频率小于5%的位点(频率的筛选主要依据疾病在人群中的发病率)
(14)筛选出突变类型为Nonsynonymous的位点。
表4可替代胰岛素治疗方案的基因小结
实施例2:
为了验证体系的稳定性以及分型结果的准确性,我们对6例随机糖尿病人血液样本提取的DNA,分别采用市场上比较常见和认可的安捷伦外显子测序试剂盒与本专利所述的基因检测试剂盒分别进行建库测序,然后对其进行突变位点检测分析。最后结果表明,利用该试剂盒得到的目标区域的基因型与安捷伦外显子测序试剂盒中该区域的基因型完全相同。说明该方法针对该区域的的准确性检测与安捷伦等全外显子测序相当。以下仅展示检测到的突变位点。
样本1:
样本2:
样本3:
样本6:
实施例3:
为了验证体系的重复性,我们对10例不同糖尿病人血液样本进行两次重复,最后的分析结果表明,两次实验找到的突变位点完全一致。以下仅展示检测到的突变位点。
样本1:
样本2:
样本3:
样本4:
样本5:
样本6:
样本7:
样本8:
样本9:
样本10:
说明本发明提供的检测方法,检测平衡性好、灵敏度高、重复性好,分型结果准确,可用于对单基因糖尿病筛查的辅助诊断工具。
以上所述、仅是本申请的几个实施例、并非对本申请做任何形式的限制、虽然本申请以较佳实施例揭示如上、然而并非用以限制本申请、任何熟悉本专业的技术人员、在不脱离本申请技术方案的范围内、利用上述揭示的技术内容做出些许的变动或修饰均等同于等效实施案例、均属于技术方案范围内。
序列表
<110> 中国科学院大学;中国科学院生物物理研究所
<120> 用于检测单基因糖尿病的相关基因的引物组、试剂盒及方法
<130> DD200282I
<141> 2020-07-15
<160> 1502
<170> SIPOSequenceListing 1.0
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ctgcttcagg gttctttctt gatta 25
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gttgtcatcc tcctcgctct ca 22
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aaggaaaact tgatgaagca gaagat 26
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ggaagtggag attatactgc tgctat 26
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aaaatacttt tattgtgatt tctgtggaaa a 31
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tgaagagggg aggagccttt c 21
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cgactttagc atcaatttta caggat 26
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ctcctctgcc caaagaaaac attcc 25
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ctcggctggt gggttgtcg 19
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gttaagcaga cagacaagca agttat 26
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actaagccat ggtagtcaat gaacta 26
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gagtccatgc ttgccacaac c 21
<210> 115
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taaacttccg agaaagttca gaccca 26
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aggagaccta cagttatttt tcaccac 27
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caggccttgt gagaagttgt gtt 23
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tcctctgaaa acccttaaac cagata 26
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aactagtgtc tcaatatccc aggacc 26
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ttgtcgatga aagggaaact gaggc 25
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gctaagggat tgggaagggt cc 22
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caactcctcc aaggcctgaa cc 22
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gtggatggcc ttgactgacg g 21
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gagctgggag gacttgctgc 20
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gtgggttcca tgctgcccg 19
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caatagtgac gaaataaccc tgctcc 26
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ggacctgtcc gagagccgc 19
<210> 128
<211> 28
<212> DNA
<213> Artifical
<400> 128
acagggacaa aataccttaa tgtatgta 28
<210> 129
<211> 26
<212> DNA
<213> Artifical
<400> 129
aatgagagct tttagtgcca atgttg 26
<210> 130
<211> 26
<212> DNA
<213> Artifical
<400> 130
ccgttcatta gctcccatgt atttca 26
<210> 131
<211> 24
<212> DNA
<213> Artifical
<400> 131
tcctgagttt tccagaggct ttca 24
<210> 132
<211> 24
<212> DNA
<213> Artifical
<400> 132
ttgacaatct ccaggaaggt tggc 24
<210> 133
<211> 23
<212> DNA
<213> Artifical
<400> 133
cacacacctt aaaccctttc tac 23
<210> 134
<211> 21
<212> DNA
<213> Artifical
<400> 134
gaggatggca ggcttccttc c 21
<210> 135
<211> 26
<212> DNA
<213> Artifical
<400> 135
acggctcatt atagacaact tccttc 26
<210> 136
<211> 23
<212> DNA
<213> Artifical
<400> 136
gagaacggag gtagctcttc agg 23
<210> 137
<211> 24
<212> DNA
<213> Artifical
<400> 137
gagaaaggga agggtcagga aagc 24
<210> 138
<211> 26
<212> DNA
<213> Artifical
<400> 138
cttcactctc actcaatggt gaaggc 26
<210> 139
<211> 26
<212> DNA
<213> Artifical
<400> 139
acttccaatc acaacactga agagaa 26
<210> 140
<211> 26
<212> DNA
<213> Artifical
<400> 140
tagtctgtca cgtagaaata ggtggg 26
<210> 141
<211> 26
<212> DNA
<213> Artifical
<400> 141
ctggaatcaa aatttcagca gaggaa 26
<210> 142
<211> 24
<212> DNA
<213> Artifical
<400> 142
gcttagaggg tggagaatct gtcc 24
<210> 143
<211> 26
<212> DNA
<213> Artifical
<400> 143
tggatttctg aattggtgaa gcatct 26
<210> 144
<211> 26
<212> DNA
<213> Artifical
<400> 144
ttcaggatct gagagtctgt ctttgg 26
<210> 145
<211> 25
<212> DNA
<213> Artifical
<400> 145
gaagagatag ctgcttccct agagg 25
<210> 146
<211> 26
<212> DNA
<213> Artifical
<400> 146
ttttatacaa cctcactgca tcagcc 26
<210> 147
<211> 23
<212> DNA
<213> Artifical
<400> 147
cagattggag cacaaacgta gca 23
<210> 148
<211> 26
<212> DNA
<213> Artifical
<400> 148
tgtaatgcac ttgaatcatg ctgaaa 26
<210> 149
<211> 26
<212> DNA
<213> Artifical
<400> 149
gtccaaggca tagaaggagt agttcc 26
<210> 150
<211> 28
<212> DNA
<213> Artifical
<400> 150
aatccttaag ttgttctaat acacgaac 28
<210> 151
<211> 23
<212> DNA
<213> Artifical
<400> 151
agggtctgca ctgcttttct tcc 23
<210> 152
<211> 23
<212> DNA
<213> Artifical
<400> 152
agttttaaca agcggcatcg tca 23
<210> 153
<211> 28
<212> DNA
<213> Artifical
<400> 153
aatgaccatt taacattttt aagccata 28
<210> 154
<211> 22
<212> DNA
<213> Artifical
<400> 154
gaggtgaacc atctcgaaga tg 22
<210> 155
<211> 22
<212> DNA
<213> Artifical
<400> 155
gagggttctc agtccacaag cg 22
<210> 156
<211> 19
<212> DNA
<213> Artifical
<400> 156
agatgaggat gggagccca 19
<210> 157
<211> 21
<212> DNA
<213> Artifical
<400> 157
aaagcaaagg agccatgggt g 21
<210> 158
<211> 20
<212> DNA
<213> Artifical
<400> 158
cagaactgga gatggagctg 20
<210> 159
<211> 20
<212> DNA
<213> Artifical
<400> 159
ctggtaatcc tggcagggag 20
<210> 160
<211> 23
<212> DNA
<213> Artifical
<400> 160
gttcatctgg tcgacctgtg agc 23
<210> 161
<211> 22
<212> DNA
<213> Artifical
<400> 161
ataatcccat cccagccaag cc 22
<210> 162
<211> 20
<212> DNA
<213> Artifical
<400> 162
ctcctgagag tagggcgggg 20
<210> 163
<211> 26
<212> DNA
<213> Artifical
<400> 163
caccaagaac actatgcata cttcgc 26
<210> 164
<211> 26
<212> DNA
<213> Artifical
<400> 164
ttcttcttcg catagtatac ctgccc 26
<210> 165
<211> 26
<212> DNA
<213> Artifical
<400> 165
attatgtgcc cagaagaatg tcttgg 26
<210> 166
<211> 26
<212> DNA
<213> Artifical
<400> 166
ccttgagaag tgagttaaga gcagat 26
<210> 167
<211> 25
<212> DNA
<213> Artifical
<400> 167
ctaagtgcca ctgtggacgt tttca 25
<210> 168
<211> 23
<212> DNA
<213> Artifical
<400> 168
ccatagtcat tgtcctccag cac 23
<210> 169
<211> 21
<212> DNA
<213> Artifical
<400> 169
agaagctcag cccaacttcc c 21
<210> 170
<211> 25
<212> DNA
<213> Artifical
<400> 170
gaaatttccc tccacccttc catct 25
<210> 171
<211> 19
<212> DNA
<213> Artifical
<400> 171
gtccaggcct cagggtcag 19
<210> 172
<211> 24
<212> DNA
<213> Artifical
<400> 172
gaactgtgtt atggaaacca agga 24
<210> 173
<211> 26
<212> DNA
<213> Artifical
<400> 173
aaaatctctc cagctgtacc ttttct 26
<210> 174
<211> 23
<212> DNA
<213> Artifical
<400> 174
ctcaatgacc aagtcccaca agc 23
<210> 175
<211> 21
<212> DNA
<213> Artifical
<400> 175
gctcctcagg ctggtaagac c 21
<210> 176
<211> 21
<212> DNA
<213> Artifical
<400> 176
cagaacacag gctgcaggag c 21
<210> 177
<211> 23
<212> DNA
<213> Artifical
<400> 177
atgactccat gtactccact tcc 23
<210> 178
<211> 23
<212> DNA
<213> Artifical
<400> 178
cttcgctccc tacttctaca ccc 23
<210> 179
<211> 20
<212> DNA
<213> Artifical
<400> 179
agagagtgag tgctccccca 20
<210> 180
<211> 19
<212> DNA
<213> Artifical
<400> 180
agcccctgtc cactgaccc 19
<210> 181
<211> 21
<212> DNA
<213> Artifical
<400> 181
cagatggagt gagcacagag g 21
<210> 182
<211> 24
<212> DNA
<213> Artifical
<400> 182
ctggggttct agaacattct ggaa 24
<210> 183
<211> 21
<212> DNA
<213> Artifical
<400> 183
ctttgctcca acatccggga c 21
<210> 184
<211> 19
<212> DNA
<213> Artifical
<400> 184
ctttcctggc ctgaccaca 19
<210> 185
<211> 21
<212> DNA
<213> Artifical
<400> 185
ctccctcctc ctccctctgg c 21
<210> 186
<211> 22
<212> DNA
<213> Artifical
<400> 186
aaggtcgtat cccagctgtt gc 22
<210> 187
<211> 24
<212> DNA
<213> Artifical
<400> 187
tttttaaagg gtgaggccac aaga 24
<210> 188
<211> 19
<212> DNA
<213> Artifical
<400> 188
ccaaggccag gaccgtagg 19
<210> 189
<211> 20
<212> DNA
<213> Artifical
<400> 189
cacaaggcat cccctcctgg 20
<210> 190
<211> 25
<212> DNA
<213> Artifical
<400> 190
acttctttcc acctgaccat tgagg 25
<210> 191
<211> 26
<212> DNA
<213> Artifical
<400> 191
taagtacaca gtggagaatg gctaca 26
<210> 192
<211> 25
<212> DNA
<213> Artifical
<400> 192
gaccctgtct accttcagtt tctgg 25
<210> 193
<211> 19
<212> DNA
<213> Artifical
<400> 193
agccctgaga cccgtggag 19
<210> 194
<211> 19
<212> DNA
<213> Artifical
<400> 194
aggatgagga agcgggacc 19
<210> 195
<211> 20
<212> DNA
<213> Artifical
<400> 195
cccagagact ccgtgacctc 20
<210> 196
<211> 17
<212> DNA
<213> Artifical
<400> 196
ccacctgcct cctctcc 17
<210> 197
<211> 24
<212> DNA
<213> Artifical
<400> 197
gctgtatcag aaggaggtga gagg 24
<210> 198
<211> 22
<212> DNA
<213> Artifical
<400> 198
gtgtattttg gttcgcctcc gc 22
<210> 199
<211> 19
<212> DNA
<213> Artifical
<400> 199
gacttcgcag gcccagacg 19
<210> 200
<211> 26
<212> DNA
<213> Artifical
<400> 200
ctgtcgaggc tcttgtttgt atgagc 26
<210> 201
<211> 19
<212> DNA
<213> Artifical
<400> 201
gtggccagag ctctgagcg 19
<210> 202
<211> 26
<212> DNA
<213> Artifical
<400> 202
gacaaagtag catgttacca gctttt 26
<210> 203
<211> 26
<212> DNA
<213> Artifical
<400> 203
acccaaatca tccaattcca gagtaa 26
<210> 204
<211> 24
<212> DNA
<213> Artifical
<400> 204
catgacgacc aagaagatcc cagg 24
<210> 205
<211> 19
<212> DNA
<213> Artifical
<400> 205
gaagcccaac gtgtgcagc 19
<210> 206
<211> 29
<212> DNA
<213> Artifical
<400> 206
tctgaataaa aatagtctct caaacctga 29
<210> 207
<211> 26
<212> DNA
<213> Artifical
<400> 207
tcatggccaa gcagtttcat ttttat 26
<210> 208
<211> 26
<212> DNA
<213> Artifical
<400> 208
aagaagtcat tgcagttaag tcagct 26
<210> 209
<211> 22
<212> DNA
<213> Artifical
<400> 209
attgtagtct ttccttctag gc 22
<210> 210
<211> 26
<212> DNA
<213> Artifical
<400> 210
gtgaacattc agaggattgg acttcc 26
<210> 211
<211> 26
<212> DNA
<213> Artifical
<400> 211
tgctaaagcc accatttttg ttttgt 26
<210> 212
<211> 26
<212> DNA
<213> Artifical
<400> 212
gcagctaatg aattcctttt cctgtc 26
<210> 213
<211> 26
<212> DNA
<213> Artifical
<400> 213
cgaatttgaa ggagttcatg gacatt 26
<210> 214
<211> 25
<212> DNA
<213> Artifical
<400> 214
gggagaaggg ttggtaagtt ttacc 25
<210> 215
<211> 26
<212> DNA
<213> Artifical
<400> 215
tcacctggat ttccactgta atgaat 26
<210> 216
<211> 26
<212> DNA
<213> Artifical
<400> 216
agtctacttt gacagagact tggtgc 26
<210> 217
<211> 21
<212> DNA
<213> Artifical
<400> 217
gattgatccg tggctttggg c 21
<210> 218
<211> 21
<212> DNA
<213> Artifical
<400> 218
gcatgtcctg gttctgctca g 21
<210> 219
<211> 23
<212> DNA
<213> Artifical
<400> 219
ttcctcagtg agtcctcctc tgc 23
<210> 220
<211> 26
<212> DNA
<213> Artifical
<400> 220
ccctgaaatc tcattgtgac cagtat 26
<210> 221
<211> 23
<212> DNA
<213> Artifical
<400> 221
gagaatgcga ctctccaaaa ccc 23
<210> 222
<211> 19
<212> DNA
<213> Artifical
<400> 222
tggagagatc cccgcaagg 19
<210> 223
<211> 22
<212> DNA
<213> Artifical
<400> 223
gatagccagg gccctagttc tg 22
<210> 224
<211> 23
<212> DNA
<213> Artifical
<400> 224
ctcctcatca gtcacagaca ccc 23
<210> 225
<211> 19
<212> DNA
<213> Artifical
<400> 225
attccaggga gggggctct 19
<210> 226
<211> 26
<212> DNA
<213> Artifical
<400> 226
tttccccttc caggtttcta gtttta 26
<210> 227
<211> 26
<212> DNA
<213> Artifical
<400> 227
tgccaactta aaagccaaaa ctagag 26
<210> 228
<211> 22
<212> DNA
<213> Artifical
<400> 228
ggacatctgg gtcttgactc cc 22
<210> 229
<211> 21
<212> DNA
<213> Artifical
<400> 229
catcaagctc ttcggcatgg c 21
<210> 230
<211> 20
<212> DNA
<213> Artifical
<400> 230
ctgaggaaga tggcgtccca 20
<210> 231
<211> 26
<212> DNA
<213> Artifical
<400> 231
taggaccttc cagacctcat aaaact 26
<210> 232
<211> 26
<212> DNA
<213> Artifical
<400> 232
ccatcaccaa gcaggaagtt atctag 26
<210> 233
<211> 27
<212> DNA
<213> Artifical
<400> 233
tttcatttct ctttctgaaa ctctgtg 27
<210> 234
<211> 26
<212> DNA
<213> Artifical
<400> 234
ctttttcttg ccttaactcc agtgtt 26
<210> 235
<211> 26
<212> DNA
<213> Artifical
<400> 235
gcttgtgaag gatgcaaggt aattaa 26
<210> 236
<211> 26
<212> DNA
<213> Artifical
<400> 236
cttccggaga acaatcagat tgaagc 26
<210> 237
<211> 26
<212> DNA
<213> Artifical
<400> 237
tgactcatac ataaagtcct tcccgc 26
<210> 238
<211> 25
<212> DNA
<213> Artifical
<400> 238
ctgtgcagga gatcacagag tatgc 25
<210> 239
<211> 20
<212> DNA
<213> Artifical
<400> 239
tagagctgcc taggcctcca 20
<210> 240
<211> 19
<212> DNA
<213> Artifical
<400> 240
ctggagaagg ctgtgcggg 19
<210> 241
<211> 26
<212> DNA
<213> Artifical
<400> 241
tacctctgct ttgttgaagt gaaact 26
<210> 242
<211> 26
<212> DNA
<213> Artifical
<400> 242
tgcacaggta aaacactaag gactaa 26
<210> 243
<211> 27
<212> DNA
<213> Artifical
<400> 243
agagcttttt caaaattttg gaagttt 27
<210> 244
<211> 26
<212> DNA
<213> Artifical
<400> 244
gtagcttttt caccctgaat gttgaa 26
<210> 245
<211> 26
<212> DNA
<213> Artifical
<400> 245
ctccacagct cctactgaag ataata 26
<210> 246
<211> 26
<212> DNA
<213> Artifical
<400> 246
acacaaatgg gaatattcct ttgttt 26
<210> 247
<211> 26
<212> DNA
<213> Artifical
<400> 247
ccgctcttcc tctctttaag aaaaca 26
<210> 248
<211> 26
<212> DNA
<213> Artifical
<400> 248
acacattcac aaaagtgagt caacat 26
<210> 249
<211> 25
<212> DNA
<213> Artifical
<400> 249
cagggtggaa atttaatgag tcagg 25
<210> 250
<211> 26
<212> DNA
<213> Artifical
<400> 250
ttgcagttga gtattgaaca tttgcc 26
<210> 251
<211> 26
<212> DNA
<213> Artifical
<400> 251
cgagtaaatc aatcagctct ggaagc 26
<210> 252
<211> 26
<212> DNA
<213> Artifical
<400> 252
ctctctctag attctcttgt tgcccc 26
<210> 253
<211> 26
<212> DNA
<213> Artifical
<400> 253
tcactagaaa acctaaaagg aggcag 26
<210> 254
<211> 26
<212> DNA
<213> Artifical
<400> 254
ggttaattga tccacagaca caagtt 26
<210> 255
<211> 26
<212> DNA
<213> Artifical
<400> 255
ttggatttgt tcttcggaca tcaagc 26
<210> 256
<211> 27
<212> DNA
<213> Artifical
<400> 256
gctctcaaga atgtatctta actgcat 27
<210> 257
<211> 23
<212> DNA
<213> Artifical
<400> 257
ctccctctgc ttttctgtct cca 23
<210> 258
<211> 24
<212> DNA
<213> Artifical
<400> 258
cagcagatct gaagaccctc atgc 24
<210> 259
<211> 24
<212> DNA
<213> Artifical
<400> 259
gatgaagaac tcaacagctg cacc 24
<210> 260
<211> 26
<212> DNA
<213> Artifical
<400> 260
gaagctcaac cccaagaaga agaagc 26
<210> 261
<211> 19
<212> DNA
<213> Artifical
<400> 261
gcccattgct ctgtgtgag 19
<210> 262
<211> 22
<212> DNA
<213> Artifical
<400> 262
gagggaggac cactaggatg gg 22
<210> 263
<211> 24
<212> DNA
<213> Artifical
<400> 263
cttcatctcc atggtgatct gcac 24
<210> 264
<211> 20
<212> DNA
<213> Artifical
<400> 264
gtcccgtgcc tgctctatgt 20
<210> 265
<211> 19
<212> DNA
<213> Artifical
<400> 265
catcctggtg tggctcacg 19
<210> 266
<211> 24
<212> DNA
<213> Artifical
<400> 266
atcaagaagt tcgaccgcta caag 24
<210> 267
<211> 19
<212> DNA
<213> Artifical
<400> 267
catggcgccg tgaagttcg 19
<210> 268
<211> 26
<212> DNA
<213> Artifical
<400> 268
actgattgtc atgagaagca ctttca 26
<210> 269
<211> 26
<212> DNA
<213> Artifical
<400> 269
gagctgtgca tgattgattc cataat 26
<210> 270
<211> 26
<212> DNA
<213> Artifical
<400> 270
attgttactt tcaggaaatg gtgata 26
<210> 271
<211> 26
<212> DNA
<213> Artifical
<400> 271
gttcagagat tgcatctgga gattgt 26
<210> 272
<211> 26
<212> DNA
<213> Artifical
<400> 272
acctgtaaga ctactgcaaa gactat 26
<210> 273
<211> 26
<212> DNA
<213> Artifical
<400> 273
caataataag gcgaagggtg ctatga 26
<210> 274
<211> 26
<212> DNA
<213> Artifical
<400> 274
tattggttca cacccttcac ctaatc 26
<210> 275
<211> 24
<212> DNA
<213> Artifical
<400> 275
ctcccagtcc ctgaaagcat tacc 24
<210> 276
<211> 25
<212> DNA
<213> Artifical
<400> 276
cactgcagat tctgacacat ctaca 25
<210> 277
<211> 26
<212> DNA
<213> Artifical
<400> 277
ctttctttct gagagcattt cacaaa 26
<210> 278
<211> 26
<212> DNA
<213> Artifical
<400> 278
ctttctttcg gagagcattt cacaaa 26
<210> 279
<211> 26
<212> DNA
<213> Artifical
<400> 279
tggaagaaat atttgttcaa ccagta 26
<210> 280
<211> 26
<212> DNA
<213> Artifical
<400> 280
ctttctttct gagagcattt cacaaa 26
<210> 281
<211> 23
<212> DNA
<213> Artifical
<400> 281
gaagccaggc ctgaagaaat tgg 23
<210> 282
<211> 26
<212> DNA
<213> Artifical
<400> 282
ccttcattgt ggtctaatgc attcaa 26
<210> 283
<211> 28
<212> DNA
<213> Artifical
<400> 283
tgagtcaaat tgttcagaaa attagccc 28
<210> 284
<211> 26
<212> DNA
<213> Artifical
<400> 284
tctttacaca ctgagctttt tgcatt 26
<210> 285
<211> 26
<212> DNA
<213> Artifical
<400> 285
ctagcatacc tcatcagtat tggctt 26
<210> 286
<211> 26
<212> DNA
<213> Artifical
<400> 286
gatgctatca cacacattca cttgag 26
<210> 287
<211> 26
<212> DNA
<213> Artifical
<400> 287
aaatgaattc caagacacca ttacaa 26
<210> 288
<211> 26
<212> DNA
<213> Artifical
<400> 288
cctcctaaac caccaaaacc tactac 26
<210> 289
<211> 22
<212> DNA
<213> Artifical
<400> 289
acagcagacg ggaccttttt gg 22
<210> 290
<211> 27
<212> DNA
<213> Artifical
<400> 290
agatgtttcc atgtcagcta ttttgtt 27
<210> 291
<211> 26
<212> DNA
<213> Artifical
<400> 291
agatcaaatg tcctggtagt gtcttg 26
<210> 292
<211> 26
<212> DNA
<213> Artifical
<400> 292
ttacctaagg aaaactgctg ggaaac 26
<210> 293
<211> 26
<212> DNA
<213> Artifical
<400> 293
ggtgattgct acaattcagg atgagt 26
<210> 294
<211> 26
<212> DNA
<213> Artifical
<400> 294
tcactggtgg aagatgatga agattt 26
<210> 295
<211> 24
<212> DNA
<213> Artifical
<400> 295
agatgccctg aagagttgtg aatt 24
<210> 296
<211> 23
<212> DNA
<213> Artifical
<400> 296
tctactccag cctcattacc cca 23
<210> 297
<211> 22
<212> DNA
<213> Artifical
<400> 297
aatggaagca gtagttgggc gg 22
<210> 298
<211> 24
<212> DNA
<213> Artifical
<400> 298
cttcccacac acagagcatg aatg 24
<210> 299
<211> 26
<212> DNA
<213> Artifical
<400> 299
ggggtcagtg aaactaattg taagct 26
<210> 300
<211> 24
<212> DNA
<213> Artifical
<400> 300
ctgcaggcag gagtatgtat gaaa 24
<210> 301
<211> 19
<212> DNA
<213> Artifical
<400> 301
gcctgggctg tacctgtgc 19
<210> 302
<211> 26
<212> DNA
<213> Artifical
<400> 302
agtctatccg gaagaaacag tgtacc 26
<210> 303
<211> 26
<212> DNA
<213> Artifical
<400> 303
ataaaaagaa gcagacacag ctcacg 26
<210> 304
<211> 26
<212> DNA
<213> Artifical
<400> 304
gaaaggtaaa tgacacgtat tggcta 26
<210> 305
<211> 29
<212> DNA
<213> Artifical
<400> 305
tggttatgtc agttatacct tagtctctt 29
<210> 306
<211> 36
<212> DNA
<213> Artifical
<400> 306
tgaaatttta tggatttgta ttttactaga atagta 36
<210> 307
<211> 26
<212> DNA
<213> Artifical
<400> 307
gttgtccatt tgtttgcttt tctccc 26
<210> 308
<211> 29
<212> DNA
<213> Artifical
<400> 308
tcatgttctg ttcttaatga gttcatgtt 29
<210> 309
<211> 35
<212> DNA
<213> Artifical
<400> 309
aaaccataga ataatagtat acctcaatta aaata 35
<210> 310
<211> 26
<212> DNA
<213> Artifical
<400> 310
agatgatcat gagtctcagc tactgc 26
<210> 311
<211> 26
<212> DNA
<213> Artifical
<400> 311
gaaagggttg atttgaacag cattgg 26
<210> 312
<211> 26
<212> DNA
<213> Artifical
<400> 312
tctatcactg tgttccaaga actgaa 26
<210> 313
<211> 26
<212> DNA
<213> Artifical
<400> 313
tttggtgctc gagtaatgca taatct 26
<210> 314
<211> 26
<212> DNA
<213> Artifical
<400> 314
gcttctcgat gaatacattc tcctgg 26
<210> 315
<211> 26
<212> DNA
<213> Artifical
<400> 315
ccatgggaaa cattgcttag tagttt 26
<210> 316
<211> 26
<212> DNA
<213> Artifical
<400> 316
tgaagcaagc tggaaaacaa tacatt 26
<210> 317
<211> 26
<212> DNA
<213> Artifical
<400> 317
gccaatcatg acttttatag caccag 26
<210> 318
<211> 26
<212> DNA
<213> Artifical
<400> 318
atgtacaagc acaaatccag ttctgt 26
<210> 319
<211> 26
<212> DNA
<213> Artifical
<400> 319
ttacacagac acatcatctc cagttg 26
<210> 320
<211> 26
<212> DNA
<213> Artifical
<400> 320
attgctctgg taaaccatac agactc 26
<210> 321
<211> 24
<212> DNA
<213> Artifical
<400> 321
tcatgccatg gagaactaca gttc 24
<210> 322
<211> 25
<212> DNA
<213> Artifical
<400> 322
caaaagcttt tgtcacaagt gctgc 25
<210> 323
<211> 26
<212> DNA
<213> Artifical
<400> 323
tagtactttg gaaagctccc attcct 26
<210> 324
<211> 23
<212> DNA
<213> Artifical
<400> 324
gtaggcgtgc agaaacttga tgc 23
<210> 325
<211> 26
<212> DNA
<213> Artifical
<400> 325
actttagtta cctggtctga tggaca 26
<210> 326
<211> 24
<212> DNA
<213> Artifical
<400> 326
cagcatacag gccttcttac aggc 24
<210> 327
<211> 20
<212> DNA
<213> Artifical
<400> 327
gtagacacgc tctcgcaggc 20
<210> 328
<211> 25
<212> DNA
<213> Artifical
<400> 328
atgtacttgc cacctatgag cttct 25
<210> 329
<211> 23
<212> DNA
<213> Artifical
<400> 329
ggtcatactc cagcaggaac tcg 23
<210> 330
<211> 21
<212> DNA
<213> Artifical
<400> 330
cttacccacg atcatgccga c 21
<210> 331
<211> 26
<212> DNA
<213> Artifical
<400> 331
gccagaatta gctaatgtga tgcttc 26
<210> 332
<211> 23
<212> DNA
<213> Artifical
<400> 332
cttgtccagg aagtcggaga tgc 23
<210> 333
<211> 25
<212> DNA
<213> Artifical
<400> 333
gagtacatct ggtgtttggt cttca 25
<210> 334
<211> 23
<212> DNA
<213> Artifical
<400> 334
catccgtctc atcaccttct tca 23
<210> 335
<211> 21
<212> DNA
<213> Artifical
<400> 335
gagaggaaca gaagggcctg g 21
<210> 336
<211> 26
<212> DNA
<213> Artifical
<400> 336
tgtgcataaa atcagattct gaggct 26
<210> 337
<211> 25
<212> DNA
<213> Artifical
<400> 337
cctcattcca agccatacag tagtg 25
<210> 338
<211> 18
<212> DNA
<213> Artifical
<400> 338
cagtggttcc agggcagg 18
<210> 339
<211> 20
<212> DNA
<213> Artifical
<400> 339
gtcttcccag ggcacagact 20
<210> 340
<211> 22
<212> DNA
<213> Artifical
<400> 340
ctgagcgggc agatggatga tg 22
<210> 341
<211> 19
<212> DNA
<213> Artifical
<400> 341
cacctctcag cctttgccc 19
<210> 342
<211> 26
<212> DNA
<213> Artifical
<400> 342
agtcctgcct cttcttcaat agatcc 26
<210> 343
<211> 26
<212> DNA
<213> Artifical
<400> 343
cccacattgc caaataagaa aggttc 26
<210> 344
<211> 19
<212> DNA
<213> Artifical
<400> 344
tcagagcctg caacagccc 19
<210> 345
<211> 26
<212> DNA
<213> Artifical
<400> 345
cagaggattt ctgtgtccta aatgca 26
<210> 346
<211> 18
<212> DNA
<213> Artifical
<400> 346
cagcgcccaa gacaagga 18
<210> 347
<211> 23
<212> DNA
<213> Artifical
<400> 347
agaagcctgt cctccttggt agc 23
<210> 348
<211> 22
<212> DNA
<213> Artifical
<400> 348
catgatctca tctctgtttc cc 22
<210> 349
<211> 26
<212> DNA
<213> Artifical
<400> 349
tcaaagcttt aaaatgaggc ccaaat 26
<210> 350
<211> 26
<212> DNA
<213> Artifical
<400> 350
gaagagaaag agttgagtgt gtgtgc 26
<210> 351
<211> 24
<212> DNA
<213> Artifical
<400> 351
ggaaactcgg gtctggacaa ttcg 24
<210> 352
<211> 24
<212> DNA
<213> Artifical
<400> 352
caaggagccc atctacattg tcac 24
<210> 353
<211> 26
<212> DNA
<213> Artifical
<400> 353
tgcaacttct cttttcttag gatgcc 26
<210> 354
<211> 26
<212> DNA
<213> Artifical
<400> 354
atacattgag cgcatgaatt ccatcc 26
<210> 355
<211> 23
<212> DNA
<213> Artifical
<400> 355
cttcaccatc aaagcagacg tgt 23
<210> 356
<211> 19
<212> DNA
<213> Artifical
<400> 356
gcagggatga gcaaccccg 19
<210> 357
<211> 26
<212> DNA
<213> Artifical
<400> 357
ggaaccacca aaaattcaaa ttggga 26
<210> 358
<211> 19
<212> DNA
<213> Artifical
<400> 358
cagtctacgt gcccacacc 19
<210> 359
<211> 19
<212> DNA
<213> Artifical
<400> 359
cttcgcgggc tcctactcc 19
<210> 360
<211> 26
<212> DNA
<213> Artifical
<400> 360
cccccaactc agtagatatg tttgac 26
<210> 361
<211> 26
<212> DNA
<213> Artifical
<400> 361
caccttttac ttggacatga agcatt 26
<210> 362
<211> 19
<212> DNA
<213> Artifical
<400> 362
acccatcccg gctgtctcg 19
<210> 363
<211> 26
<212> DNA
<213> Artifical
<400> 363
ggatcaggag aaacagagag aagtgc 26
<210> 364
<211> 26
<212> DNA
<213> Artifical
<400> 364
taatcactgc gtaatcttcc ctcttc 26
<210> 365
<211> 25
<212> DNA
<213> Artifical
<400> 365
ggataaggag aggaacagga atcct 25
<210> 366
<211> 22
<212> DNA
<213> Artifical
<400> 366
ccttcctcat gccaactcct gc 22
<210> 367
<211> 26
<212> DNA
<213> Artifical
<400> 367
ccaacttcct caacaactac ctgtat 26
<210> 368
<211> 20
<212> DNA
<213> Artifical
<400> 368
aggtctcggg atccctgtgg 20
<210> 369
<211> 20
<212> DNA
<213> Artifical
<400> 369
ggtaaacgga ggagggcagg 20
<210> 370
<211> 21
<212> DNA
<213> Artifical
<400> 370
caattccagc cctgagaggc a 21
<210> 371
<211> 26
<212> DNA
<213> Artifical
<400> 371
ctgagtgtca gtgcctactt gattcc 26
<210> 372
<211> 26
<212> DNA
<213> Artifical
<400> 372
gaatttggtt ctcagctttc ccttcc 26
<210> 373
<211> 24
<212> DNA
<213> Artifical
<400> 373
aggacagtct ctaaggccat gatc 24
<210> 374
<211> 26
<212> DNA
<213> Artifical
<400> 374
tacctggaga tcaccaagaa gatggg 26
<210> 375
<211> 26
<212> DNA
<213> Artifical
<400> 375
tacctggaga tcaccaagaa gatggg 26
<210> 376
<211> 23
<212> DNA
<213> Artifical
<400> 376
cccacagatg actccaagga agc 23
<210> 377
<211> 26
<212> DNA
<213> Artifical
<400> 377
gaaataaggc ctacttcaca aagcgc 26
<210> 378
<211> 23
<212> DNA
<213> Artifical
<400> 378
gacctgcagg agctcaatga tcg 23
<210> 379
<211> 25
<212> DNA
<213> Artifical
<400> 379
caggaggata caggagagca tttgc 25
<210> 380
<211> 24
<212> DNA
<213> Artifical
<400> 380
ctgtgagcac tagaggattt cccg 24
<210> 381
<211> 26
<212> DNA
<213> Artifical
<400> 381
tttagcaata ccaagaagga gggtga 26
<210> 382
<211> 24
<212> DNA
<213> Artifical
<400> 382
cttttcctca tctctgcctg cttc 24
<210> 383
<211> 26
<212> DNA
<213> Artifical
<400> 383
cagtataaga aggagctgga gaagac 26
<210> 384
<211> 20
<212> DNA
<213> Artifical
<400> 384
catcgacagc ctctctgccc 20
<210> 385
<211> 22
<212> DNA
<213> Artifical
<400> 385
caggagcttc tggacatcaa gc 22
<210> 386
<211> 22
<212> DNA
<213> Artifical
<400> 386
gctgggcctt tgagcaagat ac 22
<210> 387
<211> 22
<212> DNA
<213> Artifical
<400> 387
ggtggtcact ggggtagaca tg 22
<210> 388
<211> 21
<212> DNA
<213> Artifical
<400> 388
catctcctct ggctcttctg c 21
<210> 389
<211> 23
<212> DNA
<213> Artifical
<400> 389
gattccacta cacctggctg agg 23
<210> 390
<211> 26
<212> DNA
<213> Artifical
<400> 390
ttcttgaatt cgtttctgga acctgc 26
<210> 391
<211> 22
<212> DNA
<213> Artifical
<400> 391
caatggactc agatggggtc tt 22
<210> 392
<211> 26
<212> DNA
<213> Artifical
<400> 392
caccttctgg atcagaactt gtttcc 26
<210> 393
<211> 26
<212> DNA
<213> Artifical
<400> 393
tttcatgcga agatggtgaa tttctg 26
<210> 394
<211> 22
<212> DNA
<213> Artifical
<400> 394
cgagtattgc cagtggatcc cc 22
<210> 395
<211> 22
<212> DNA
<213> Artifical
<400> 395
gggaacttag aagagcctgc tt 22
<210> 396
<211> 26
<212> DNA
<213> Artifical
<400> 396
ggaaggctca ttgttaagtc caaaag 26
<210> 397
<211> 26
<212> DNA
<213> Artifical
<400> 397
aaatgaaact gcttgaagat ggcatc 26
<210> 398
<211> 21
<212> DNA
<213> Artifical
<400> 398
cgtgtttgca aacggggaag c 21
<210> 399
<211> 20
<212> DNA
<213> Artifical
<400> 399
ccgcttctgt ggcacataca 20
<210> 400
<211> 22
<212> DNA
<213> Artifical
<400> 400
ctgcttccct taaggtggct ca 22
<210> 401
<211> 19
<212> DNA
<213> Artifical
<400> 401
gtttgtgaaa cccaagcag 19
<210> 402
<211> 23
<212> DNA
<213> Artifical
<400> 402
tggaaggttc tgttctgagt gcc 23
<210> 403
<211> 19
<212> DNA
<213> Artifical
<400> 403
ctcccagggt tcgtgctcc 19
<210> 404
<211> 20
<212> DNA
<213> Artifical
<400> 404
gggctggttc aagggcttta 20
<210> 405
<211> 18
<212> DNA
<213> Artifical
<400> 405
cccttctgcc catgctgg 18
<210> 406
<211> 26
<212> DNA
<213> Artifical
<400> 406
aaatagcata gtgacaagtg ccttgt 26
<210> 407
<211> 21
<212> DNA
<213> Artifical
<400> 407
gagtggtgtg ggcactttga c 21
<210> 408
<211> 24
<212> DNA
<213> Artifical
<400> 408
ctgatgatca tgctcttgcg gagg 24
<210> 409
<211> 19
<212> DNA
<213> Artifical
<400> 409
ttgaggtcca ccagcgtgg 19
<210> 410
<211> 19
<212> DNA
<213> Artifical
<400> 410
gtccttccgg ctgagcagc 19
<210> 411
<211> 26
<212> DNA
<213> Artifical
<400> 411
actacttcgt gcaaatttct ccctag 26
<210> 412
<211> 21
<212> DNA
<213> Artifical
<400> 412
gagacacggg cttctgtctg c 21
<210> 413
<211> 21
<212> DNA
<213> Artifical
<400> 413
ggatgatgga gaggcgtgag c 21
<210> 414
<211> 24
<212> DNA
<213> Artifical
<400> 414
gaagaaggca agagagaagg agga 24
<210> 415
<211> 25
<212> DNA
<213> Artifical
<400> 415
ctcaggttct ggatctggat cttcc 25
<210> 416
<211> 24
<212> DNA
<213> Artifical
<400> 416
gagtgtctca tgtctccagt gacg 24
<210> 417
<211> 26
<212> DNA
<213> Artifical
<400> 417
tatcctatcc tctctttcat ccccag 26
<210> 418
<211> 26
<212> DNA
<213> Artifical
<400> 418
agtggagtcc tgagaatcaa atctca 26
<210> 419
<211> 25
<212> DNA
<213> Artifical
<400> 419
cttctggatg aagtagcaca cgatg 25
<210> 420
<211> 22
<212> DNA
<213> Artifical
<400> 420
cagtttctcc ctgcttcttg ca 22
<210> 421
<211> 22
<212> DNA
<213> Artifical
<400> 421
cagtctcttg gccaccttca gc 22
<210> 422
<211> 22
<212> DNA
<213> Artifical
<400> 422
gacaggttgt catcctcctc gc 22
<210> 423
<211> 24
<212> DNA
<213> Artifical
<400> 423
taccttcctc ctcttcctca tcct 24
<210> 424
<211> 26
<212> DNA
<213> Artifical
<400> 424
atccttcatg gcaatgatct ggaaag 26
<210> 425
<211> 26
<212> DNA
<213> Artifical
<400> 425
taagtggtca ctcagatgga tatcca 26
<210> 426
<211> 25
<212> DNA
<213> Artifical
<400> 426
accaaagagg aggaacacat catgc 25
<210> 427
<211> 20
<212> DNA
<213> Artifical
<400> 427
tgggtgatcg atggaggggc 20
<210> 428
<211> 26
<212> DNA
<213> Artifical
<400> 428
gtctctgaaa atatgtaggc tgcaca 26
<210> 429
<211> 26
<212> DNA
<213> Artifical
<400> 429
tgctcaataa atgcagcttt gtcttt 26
<210> 430
<211> 21
<212> DNA
<213> Artifical
<400> 430
ctgggagctg tgtagggaag g 21
<210> 431
<211> 22
<212> DNA
<213> Artifical
<400> 431
gaccaaacag ctgtggtttg gc 22
<210> 432
<211> 26
<212> DNA
<213> Artifical
<400> 432
ctttcgatcc tattttcagt ctggct 26
<210> 433
<211> 26
<212> DNA
<213> Artifical
<400> 433
actcactgga gatggaggta tagatg 26
<210> 434
<211> 26
<212> DNA
<213> Artifical
<400> 434
gactccgagt atgtagtaga ggagaa 26
<210> 435
<211> 26
<212> DNA
<213> Artifical
<400> 435
attacagatc tgtccagcag tcattt 26
<210> 436
<211> 26
<212> DNA
<213> Artifical
<400> 436
gtttcaatgg ccacatagta ggatgc 26
<210> 437
<211> 21
<212> DNA
<213> Artifical
<400> 437
ccaagatgcg gaaagtgctg c 21
<210> 438
<211> 24
<212> DNA
<213> Artifical
<400> 438
agtgaataga tggtgtggct gtgc 24
<210> 439
<211> 19
<212> DNA
<213> Artifical
<400> 439
gctgagaagc agggtgggg 19
<210> 440
<211> 26
<212> DNA
<213> Artifical
<400> 440
ctatcctcct tcaccagact gtaagc 26
<210> 441
<211> 26
<212> DNA
<213> Artifical
<400> 441
caaaagacat gggcttgtgg aatata 26
<210> 442
<211> 25
<212> DNA
<213> Artifical
<400> 442
gaagcaaaac ttggtgatcc catgg 25
<210> 443
<211> 25
<212> DNA
<213> Artifical
<400> 443
ggatggggaa ggtgatgaag agtag 25
<210> 444
<211> 26
<212> DNA
<213> Artifical
<400> 444
ctttctttcc tgaaagctca actgtt 26
<210> 445
<211> 26
<212> DNA
<213> Artifical
<400> 445
ataagaccag gagattctgt ttgggt 26
<210> 446
<211> 20
<212> DNA
<213> Artifical
<400> 446
acacagccaa tgaggtccgc 20
<210> 447
<211> 26
<212> DNA
<213> Artifical
<400> 447
tttttcttct cttctccatt tggccc 26
<210> 448
<211> 26
<212> DNA
<213> Artifical
<400> 448
ttgctcttgg gtaaaggatg ttctat 26
<210> 449
<211> 26
<212> DNA
<213> Artifical
<400> 449
aggttgcgaa gaactctgtt tattga 26
<210> 450
<211> 23
<212> DNA
<213> Artifical
<400> 450
tttactacca ccgattgccc tgg 23
<210> 451
<211> 26
<212> DNA
<213> Artifical
<400> 451
tatgtaactg acccaggttg aaagag 26
<210> 452
<211> 23
<212> DNA
<213> Artifical
<400> 452
aaagacacca ccgagctgat tca 23
<210> 453
<211> 26
<212> DNA
<213> Artifical
<400> 453
atatgaacct tggagaattt ccccag 26
<210> 454
<211> 19
<212> DNA
<213> Artifical
<400> 454
ctgcagacgg agctcctgg 19
<210> 455
<211> 21
<212> DNA
<213> Artifical
<400> 455
ttgacaaggt tccagcaccc a 21
<210> 456
<211> 24
<212> DNA
<213> Artifical
<400> 456
aaagaatcaa gggcaaggtc aggg 24
<210> 457
<211> 24
<212> DNA
<213> Artifical
<400> 457
cttctcagaa ccctcccctt catg 24
<210> 458
<211> 23
<212> DNA
<213> Artifical
<400> 458
atggagtttg aagtgctgag ggc 23
<210> 459
<211> 22
<212> DNA
<213> Artifical
<400> 459
cagccccaga acctcatcat gg 22
<210> 460
<211> 20
<212> DNA
<213> Artifical
<400> 460
cctgtgcaga gccatgtgac 20
<210> 461
<211> 24
<212> DNA
<213> Artifical
<400> 461
gaaaatcagc cctggatctc caac 24
<210> 462
<211> 19
<212> DNA
<213> Artifical
<400> 462
ctgtgacaga gcccctcac 19
<210> 463
<211> 22
<212> DNA
<213> Artifical
<400> 463
ccccatcctg agtaccccta gg 22
<210> 464
<211> 24
<212> DNA
<213> Artifical
<400> 464
ctgtgggttc cctctgagat cagt 24
<210> 465
<211> 23
<212> DNA
<213> Artifical
<400> 465
cagctttaca aggacccatg cgc 23
<210> 466
<211> 19
<212> DNA
<213> Artifical
<400> 466
tgtacatggg ccgccagcc 19
<210> 467
<211> 26
<212> DNA
<213> Artifical
<400> 467
tttcccatgg atgaagtcta ccaaag 26
<210> 468
<211> 23
<212> DNA
<213> Artifical
<400> 468
gctagagctg gagaaggagt tcc 23
<210> 469
<211> 19
<212> DNA
<213> Artifical
<400> 469
ccacagccct ccagcgtcg 19
<210> 470
<211> 21
<212> DNA
<213> Artifical
<400> 470
gaaccacgat gagaggcagg a 21
<210> 471
<211> 20
<212> DNA
<213> Artifical
<400> 471
actgaggcct gagcgagagc 20
<210> 472
<211> 26
<212> DNA
<213> Artifical
<400> 472
tttgactgat taggtcaaga tgggta 26
<210> 473
<211> 25
<212> DNA
<213> Artifical
<400> 473
tgctgatact gtccaggtga tttgt 25
<210> 474
<211> 23
<212> DNA
<213> Artifical
<400> 474
gtgtcaagtc agatgtgcac tgg 23
<210> 475
<211> 27
<212> DNA
<213> Artifical
<400> 475
gtatttggaa tggtttaccc ttgtgta 27
<210> 476
<211> 26
<212> DNA
<213> Artifical
<400> 476
gaggtaagaa aagggccttg ttatta 26
<210> 477
<211> 29
<212> DNA
<213> Artifical
<400> 477
tcgagctgaa tgttttataa aagaaggag 29
<210> 478
<211> 26
<212> DNA
<213> Artifical
<400> 478
acttgaccag gatcataaaa ggtgtt 26
<210> 479
<211> 26
<212> DNA
<213> Artifical
<400> 479
tgaaaactca acatgtggaa taccaa 26
<210> 480
<211> 26
<212> DNA
<213> Artifical
<400> 480
gcacaaagat tattgaaaca gtcgca 26
<210> 481
<211> 26
<212> DNA
<213> Artifical
<400> 481
acccagataa cttccagaat gaagaa 26
<210> 482
<211> 26
<212> DNA
<213> Artifical
<400> 482
ctcaggcgga ccatttagat ttaaat 26
<210> 483
<211> 19
<212> DNA
<213> Artifical
<400> 483
gggccggaag aagctgtcg 19
<210> 484
<211> 23
<212> DNA
<213> Artifical
<400> 484
gcagcccctt cagtcaaatc tac 23
<210> 485
<211> 22
<212> DNA
<213> Artifical
<400> 485
cctgagttca ccctatgcct ct 22
<210> 486
<211> 20
<212> DNA
<213> Artifical
<400> 486
gggggatggg agggactagg 20
<210> 487
<211> 21
<212> DNA
<213> Artifical
<400> 487
tgagccacct cctgctgatt c 21
<210> 488
<211> 22
<212> DNA
<213> Artifical
<400> 488
gacttccagt cacggcatca gc 22
<210> 489
<211> 26
<212> DNA
<213> Artifical
<400> 489
cttctcatag gcattgcaca cagagg 26
<210> 490
<211> 24
<212> DNA
<213> Artifical
<400> 490
cctcctgaga gtggattgtc tgag 24
<210> 491
<211> 26
<212> DNA
<213> Artifical
<400> 491
cttgaagaca tgttggtgag tgtact 26
<210> 492
<211> 26
<212> DNA
<213> Artifical
<400> 492
ttactgtgtg agttctgcag ctgagt 26
<210> 493
<211> 26
<212> DNA
<213> Artifical
<400> 493
tgtcatgatg aggttttgag attgct 26
<210> 494
<211> 26
<212> DNA
<213> Artifical
<400> 494
tgaggaggaa gtgatctcat tgtttc 26
<210> 495
<211> 25
<212> DNA
<213> Artifical
<400> 495
ctgtgagtct ggttggagct atagg 25
<210> 496
<211> 23
<212> DNA
<213> Artifical
<400> 496
atcttcttgt tggtgggctc aga 23
<210> 497
<211> 26
<212> DNA
<213> Artifical
<400> 497
gatctctcgt tgctttctga cgtacc 26
<210> 498
<211> 19
<212> DNA
<213> Artifical
<400> 498
gttgagcgcc tgcagctcc 19
<210> 499
<211> 26
<212> DNA
<213> Artifical
<400> 499
tgagaagtca gatcccattt gaacta 26
<210> 500
<211> 22
<212> DNA
<213> Artifical
<400> 500
atctcttcct cgtcctcctc ct 22
<210> 501
<211> 23
<212> DNA
<213> Artifical
<400> 501
ggaggagatg taccagaccc tcg 23
<210> 502
<211> 19
<212> DNA
<213> Artifical
<400> 502
gacagccctc catacggca 19
<210> 503
<211> 21
<212> DNA
<213> Artifical
<400> 503
gcttccccta ctctcccagc c 21
<210> 504
<211> 19
<212> DNA
<213> Artifical
<400> 504
agcccctact cgccctacg 19
<210> 505
<211> 26
<212> DNA
<213> Artifical
<400> 505
caatagtgac gaaataaccc tgctcc 26
<210> 506
<211> 19
<212> DNA
<213> Artifical
<400> 506
catccagacg ccgctgtgg 19
<210> 507
<211> 26
<212> DNA
<213> Artifical
<400> 507
tgtactgttt ctagccttca tcacgg 26
<210> 508
<211> 26
<212> DNA
<213> Artifical
<400> 508
tgccaacctt aacagtagag catatg 26
<210> 509
<211> 22
<212> DNA
<213> Artifical
<400> 509
gagagcacca atcccgagaa ca 22
<210> 510
<211> 20
<212> DNA
<213> Artifical
<400> 510
ctgacagtgc gaggaacggt 20
<210> 511
<211> 26
<212> DNA
<213> Artifical
<400> 511
acatatggga actcaagtgt gtgtac 26
<210> 512
<211> 26
<212> DNA
<213> Artifical
<400> 512
tgttcattag acaggccttg gtaagg 26
<210> 513
<211> 26
<212> DNA
<213> Artifical
<400> 513
aatccgtttc atagatgtct ctggtc 26
<210> 514
<211> 23
<212> DNA
<213> Artifical
<400> 514
atctcttgaa gggtaggggg agg 23
<210> 515
<211> 26
<212> DNA
<213> Artifical
<400> 515
ttccagaaag tgatgagaca gtgata 26
<210> 516
<211> 23
<212> DNA
<213> Artifical
<400> 516
cttgatgatg tccctggcat tgc 23
<210> 517
<211> 26
<212> DNA
<213> Artifical
<400> 517
gcatgttaca catcctatat cagcaa 26
<210> 518
<211> 22
<212> DNA
<213> Artifical
<400> 518
ctccaagtca tcccctgcaa tg 22
<210> 519
<211> 26
<212> DNA
<213> Artifical
<400> 519
ctggaatcaa aatttcagca gaggaa 26
<210> 520
<211> 24
<212> DNA
<213> Artifical
<400> 520
gctgtaagga gaggaagtga gagg 24
<210> 521
<211> 25
<212> DNA
<213> Artifical
<400> 521
ccttgttcac caccttctca aaagg 25
<210> 522
<211> 26
<212> DNA
<213> Artifical
<400> 522
ctacctctcg gtccctaagt aatgac 26
<210> 523
<211> 24
<212> DNA
<213> Artifical
<400> 523
gccctggaga aagaaacaga aaag 24
<210> 524
<211> 25
<212> DNA
<213> Artifical
<400> 524
ctcacctttg aggcaataat ccagc 25
<210> 525
<211> 23
<212> DNA
<213> Artifical
<400> 525
gttctgtgat ttggggtcgt tgg 23
<210> 526
<211> 26
<212> DNA
<213> Artifical
<400> 526
ccatctcagc aagatcttgt caaaag 26
<210> 527
<211> 20
<212> DNA
<213> Artifical
<400> 527
gatgcctggt ccccattggt 20
<210> 528
<211> 26
<212> DNA
<213> Artifical
<400> 528
catgcactat cacacttggc taattt 26
<210> 529
<211> 26
<212> DNA
<213> Artifical
<400> 529
taggatcggc ggatttttag ataccc 26
<210> 530
<211> 26
<212> DNA
<213> Artifical
<400> 530
tcctcgaatg ttgatgatca gactcc 26
<210> 531
<211> 22
<212> DNA
<213> Artifical
<400> 531
cctggaagtg gtagtacggg gg 22
<210> 532
<211> 23
<212> DNA
<213> Artifical
<400> 532
caacacgatg taattatcct cca 23
<210> 533
<211> 23
<212> DNA
<213> Artifical
<400> 533
atcaagagta tctgcaagtg tcc 23
<210> 534
<211> 20
<212> DNA
<213> Artifical
<400> 534
ccccagactc accctctccg 20
<210> 535
<211> 20
<212> DNA
<213> Artifical
<400> 535
tgaggtgggg agcagtcagc 20
<210> 536
<211> 23
<212> DNA
<213> Artifical
<400> 536
ttgtgtgaag acccagtaac cag 23
<210> 537
<211> 20
<212> DNA
<213> Artifical
<400> 537
ctggaagcgg aggtagtcgg 20
<210> 538
<211> 19
<212> DNA
<213> Artifical
<400> 538
caagtgtgag tgaggggcc 19
<210> 539
<211> 25
<212> DNA
<213> Artifical
<400> 539
gtagaagcgg ctctggatat actgg 25
<210> 540
<211> 20
<212> DNA
<213> Artifical
<400> 540
ccagaaagag cagtgccgtg 20
<210> 541
<211> 26
<212> DNA
<213> Artifical
<400> 541
tttgttcttg atgatcttga tggcca 26
<210> 542
<211> 23
<212> DNA
<213> Artifical
<400> 542
aatgttagtg gggccttgga tca 23
<210> 543
<211> 26
<212> DNA
<213> Artifical
<400> 543
ctcattgatg tgcttgtagg tcttga 26
<210> 544
<211> 26
<212> DNA
<213> Artifical
<400> 544
ggacacaaac caaaaaggct aagtaa 26
<210> 545
<211> 21
<212> DNA
<213> Artifical
<400> 545
gaggcctcaa ccaaggtcac c 21
<210> 546
<211> 24
<212> DNA
<213> Artifical
<400> 546
ctctgcttgg ggtccttctt aagc 24
<210> 547
<211> 21
<212> DNA
<213> Artifical
<400> 547
tcccacagaa ggttttcatg g 21
<210> 548
<211> 26
<212> DNA
<213> Artifical
<400> 548
gagacaatct ctgcaccata aaaccg 26
<210> 549
<211> 26
<212> DNA
<213> Artifical
<400> 549
ctttgtacca gaagattagg gctctc 26
<210> 550
<211> 24
<212> DNA
<213> Artifical
<400> 550
ccttggcaat gatgacttcc ttcc 24
<210> 551
<211> 23
<212> DNA
<213> Artifical
<400> 551
cacttccatc tcctcagtcg tgg 23
<210> 552
<211> 23
<212> DNA
<213> Artifical
<400> 552
gtcttcatca gctggcattc tgc 23
<210> 553
<211> 26
<212> DNA
<213> Artifical
<400> 553
gatgtattca cctgaaatga ggcagg 26
<210> 554
<211> 20
<212> DNA
<213> Artifical
<400> 554
atggtgggtg catgggatgc 20
<210> 555
<211> 26
<212> DNA
<213> Artifical
<400> 555
tgaccctcat tctcttccaa gtttcc 26
<210> 556
<211> 23
<212> DNA
<213> Artifical
<400> 556
aaggtatttc gaggctgtgg aga 23
<210> 557
<211> 22
<212> DNA
<213> Artifical
<400> 557
ctgcacctct gatcatccct cc 22
<210> 558
<211> 19
<212> DNA
<213> Artifical
<400> 558
tggaacaggg catccgtgt 19
<210> 559
<211> 20
<212> DNA
<213> Artifical
<400> 559
gtcagtcacc caccggaagg 20
<210> 560
<211> 21
<212> DNA
<213> Artifical
<400> 560
catcttccct gagcctgagc g 21
<210> 561
<211> 26
<212> DNA
<213> Artifical
<400> 561
cccctagggt tgttataagg atgttg 26
<210> 562
<211> 22
<212> DNA
<213> Artifical
<400> 562
gagtacaagc tccgctccta ca 22
<210> 563
<211> 20
<212> DNA
<213> Artifical
<400> 563
atgggaacga ccagacccgc 20
<210> 564
<211> 23
<212> DNA
<213> Artifical
<400> 564
gatgcacttt ttctccccac tcc 23
<210> 565
<211> 22
<212> DNA
<213> Artifical
<400> 565
gctgtacccg tccatcatga tg 22
<210> 566
<211> 23
<212> DNA
<213> Artifical
<400> 566
gagtttgtga agacctcagt gcg 23
<210> 567
<211> 20
<212> DNA
<213> Artifical
<400> 567
aaggagacag accccctccg 20
<210> 568
<211> 23
<212> DNA
<213> Artifical
<400> 568
catgattctc tccccgacag agc 23
<210> 569
<211> 23
<212> DNA
<213> Artifical
<400> 569
gaagggaccc tgcttctcac ata 23
<210> 570
<211> 23
<212> DNA
<213> Artifical
<400> 570
ggtctacttc ccatacctgc tta 23
<210> 571
<211> 22
<212> DNA
<213> Artifical
<400> 571
gtcatcacca aggagctgac cc 22
<210> 572
<211> 24
<212> DNA
<213> Artifical
<400> 572
ctgagagccc tacagactct gtgg 24
<210> 573
<211> 19
<212> DNA
<213> Artifical
<400> 573
cccctcctgc gcatcttcg 19
<210> 574
<211> 24
<212> DNA
<213> Artifical
<400> 574
ctttcagaag ctgggattgg cagt 24
<210> 575
<211> 19
<212> DNA
<213> Artifical
<400> 575
aacagccccc acccctctc 19
<210> 576
<211> 19
<212> DNA
<213> Artifical
<400> 576
gcggccgctt tgttgctcc 19
<210> 577
<211> 22
<212> DNA
<213> Artifical
<400> 577
gcatatccac atgcccatca tg 22
<210> 578
<211> 26
<212> DNA
<213> Artifical
<400> 578
ctgtgtaaag gtattgggag cattgt 26
<210> 579
<211> 26
<212> DNA
<213> Artifical
<400> 579
cagtttactc agcactaact tggtgt 26
<210> 580
<211> 24
<212> DNA
<213> Artifical
<400> 580
gaataccaag ttgttgcccc ttgc 24
<210> 581
<211> 22
<212> DNA
<213> Artifical
<400> 581
aggggagaag cctttcaact gc 22
<210> 582
<211> 23
<212> DNA
<213> Artifical
<400> 582
gaaagtctgg gcctcgaaat tcg 23
<210> 583
<211> 26
<212> DNA
<213> Artifical
<400> 583
aggtgacatt atgttgcttt gaaaat 26
<210> 584
<211> 26
<212> DNA
<213> Artifical
<400> 584
ccttgtctta attctctagt tgggca 26
<210> 585
<211> 26
<212> DNA
<213> Artifical
<400> 585
atgtccatat ggggatatag aggaaa 26
<210> 586
<211> 26
<212> DNA
<213> Artifical
<400> 586
actacatgct agaacagatg gatttt 26
<210> 587
<211> 26
<212> DNA
<213> Artifical
<400> 587
ttagtgctcc agtatgagaa tccagt 26
<210> 588
<211> 26
<212> DNA
<213> Artifical
<400> 588
gtgagatgct tttagagtaa ggcact 26
<210> 589
<211> 28
<212> DNA
<213> Artifical
<400> 589
caaagaaagg ttgaattcag ttaatgaa 28
<210> 590
<211> 26
<212> DNA
<213> Artifical
<400> 590
actgatctat cacttgggtt agattt 26
<210> 591
<211> 26
<212> DNA
<213> Artifical
<400> 591
tacttaaact ttggcacctt gatggt 26
<210> 592
<211> 34
<212> DNA
<213> Artifical
<400> 592
agaatactta aactataaat acagaaataa gcct 34
<210> 593
<211> 26
<212> DNA
<213> Artifical
<400> 593
agtgtatgtt tgaagatccc tagcat 26
<210> 594
<211> 24
<212> DNA
<213> Artifical
<400> 594
caaagggctg ccttttgtaa acac 24
<210> 595
<211> 26
<212> DNA
<213> Artifical
<400> 595
aatcagtcag agggctttca aagaag 26
<210> 596
<211> 20
<212> DNA
<213> Artifical
<400> 596
ttgcgcaggt tgtctagcgc 20
<210> 597
<211> 26
<212> DNA
<213> Artifical
<400> 597
gaatcaataa gataaccggg cggtgg 26
<210> 598
<211> 26
<212> DNA
<213> Artifical
<400> 598
gatcttccca gaggacggtt tgaaag 26
<210> 599
<211> 23
<212> DNA
<213> Artifical
<400> 599
gacccaaatg caggactctg ttg 23
<210> 600
<211> 21
<212> DNA
<213> Artifical
<400> 600
aaacactacg gtgcctcgag c 21
<210> 601
<211> 26
<212> DNA
<213> Artifical
<400> 601
ctgatgggtg gatcactctt atagcc 26
<210> 602
<211> 24
<212> DNA
<213> Artifical
<400> 602
cgaaggtcaa gctatgagga cagc 24
<210> 603
<211> 24
<212> DNA
<213> Artifical
<400> 603
cagatgtgtg tgagtccatg aagg 24
<210> 604
<211> 22
<212> DNA
<213> Artifical
<400> 604
ccttcccaag ggtacagatg gc 22
<210> 605
<211> 26
<212> DNA
<213> Artifical
<400> 605
tgacaatgag tatgcctacc tcaaag 26
<210> 606
<211> 24
<212> DNA
<213> Artifical
<400> 606
tgagcttgga ggactacatc aacg 24
<210> 607
<211> 25
<212> DNA
<213> Artifical
<400> 607
ctccatcctg atcgaccttc tctac 25
<210> 608
<211> 26
<212> DNA
<213> Artifical
<400> 608
ttactcccac aaaggctgga attttg 26
<210> 609
<211> 27
<212> DNA
<213> Artifical
<400> 609
acttcaagtc tttttctttt aacggat 27
<210> 610
<211> 26
<212> DNA
<213> Artifical
<400> 610
cagcatttct actccacatt acgaag 26
<210> 611
<211> 26
<212> DNA
<213> Artifical
<400> 611
tatcctctca catgtctcca tacaca 26
<210> 612
<211> 19
<212> DNA
<213> Artifical
<400> 612
ccgcagctgg atgtggtca 19
<210> 613
<211> 23
<212> DNA
<213> Artifical
<400> 613
cttctactgt gtgggaacga ggg 23
<210> 614
<211> 23
<212> DNA
<213> Artifical
<400> 614
agttttctga acctgggatg gca 23
<210> 615
<211> 26
<212> DNA
<213> Artifical
<400> 615
gaaagccttt tggtgacttt atggag 26
<210> 616
<211> 26
<212> DNA
<213> Artifical
<400> 616
aaatgacaga cctcagacag attgtc 26
<210> 617
<211> 20
<212> DNA
<213> Artifical
<400> 617
ggatcgacaa gctgcccatc 20
<210> 618
<211> 26
<212> DNA
<213> Artifical
<400> 618
tcctacgatt tttgcttcct ttagaa 26
<210> 619
<211> 35
<212> DNA
<213> Artifical
<400> 619
aaaaacatgt acaatattta tgttaagtga taata 35
<210> 620
<211> 33
<212> DNA
<213> Artifical
<400> 620
tcagaaccat ttttactaga ttatgtttaa ttt 33
<210> 621
<211> 26
<212> DNA
<213> Artifical
<400> 621
gtggtcatgt ggtaggaaat tcttgc 26
<210> 622
<211> 26
<212> DNA
<213> Artifical
<400> 622
ttagaacctc tacttgggta catgca 26
<210> 623
<211> 26
<212> DNA
<213> Artifical
<400> 623
tccccactct ccagataagt ttcttt 26
<210> 624
<211> 26
<212> DNA
<213> Artifical
<400> 624
ataatctgtt ctgtgtcagt gttcgc 26
<210> 625
<211> 26
<212> DNA
<213> Artifical
<400> 625
aagccagttc attgtaacat gcttta 26
<210> 626
<211> 26
<212> DNA
<213> Artifical
<400> 626
tggttgtgtt ttgtttttgt tctttt 26
<210> 627
<211> 29
<212> DNA
<213> Artifical
<400> 627
gcattctaac aattcagagt tacattcaa 29
<210> 628
<211> 32
<212> DNA
<213> Artifical
<400> 628
acttctcatt gacttataat attgtgtttt ct 32
<210> 629
<211> 25
<212> DNA
<213> Artifical
<400> 629
taataggatg gggcatttta gagct 25
<210> 630
<211> 26
<212> DNA
<213> Artifical
<400> 630
atgttgttta tgaaactatg cgccaa 26
<210> 631
<211> 26
<212> DNA
<213> Artifical
<400> 631
gaacttagca tatgagtttg tcacaa 26
<210> 632
<211> 26
<212> DNA
<213> Artifical
<400> 632
gggcttgagc attcttaaat atgctt 26
<210> 633
<211> 28
<212> DNA
<213> Artifical
<400> 633
gaaaggttac catgtgatat taactgaa 28
<210> 634
<211> 26
<212> DNA
<213> Artifical
<400> 634
ttccactagg acttggaaga acaaag 26
<210> 635
<211> 20
<212> DNA
<213> Artifical
<400> 635
gagcaggaga ggagcgaaag 20
<210> 636
<211> 18
<212> DNA
<213> Artifical
<400> 636
gtgaaaggag gtgggctg 18
<210> 637
<211> 21
<212> DNA
<213> Artifical
<400> 637
cagagttggc agggtcagag t 21
<210> 638
<211> 23
<212> DNA
<213> Artifical
<400> 638
ctaggaacag tgcgccagtt tct 23
<210> 639
<211> 26
<212> DNA
<213> Artifical
<400> 639
ctggatgact ttgtggagat cactaa 26
<210> 640
<211> 23
<212> DNA
<213> Artifical
<400> 640
ctttccccca ggtggtcaag tac 23
<210> 641
<211> 20
<212> DNA
<213> Artifical
<400> 641
ctcggagctg gctgtcatca 20
<210> 642
<211> 26
<212> DNA
<213> Artifical
<400> 642
ctacttcctc ttcctctttg ccctcc 26
<210> 643
<211> 22
<212> DNA
<213> Artifical
<400> 643
tgactgacat cgacaacagc gc 22
<210> 644
<211> 21
<212> DNA
<213> Artifical
<400> 644
gagttcagca ccatcctgga g 21
<210> 645
<211> 33
<212> DNA
<213> Artifical
<400> 645
aaaataaaat gttcttccaa tttcaatatt cta 33
<210> 646
<211> 26
<212> DNA
<213> Artifical
<400> 646
acaattttgt ctccaggaag gtgtaa 26
<210> 647
<211> 26
<212> DNA
<213> Artifical
<400> 647
atactttgct tagctcagaa ggtgac 26
<210> 648
<211> 26
<212> DNA
<213> Artifical
<400> 648
agttcactat agtgctctgg tttgat 26
<210> 649
<211> 26
<212> DNA
<213> Artifical
<400> 649
agttgaccca tcctttgtca ttttca 26
<210> 650
<211> 26
<212> DNA
<213> Artifical
<400> 650
tgctagctaa tttaacttag gggaaa 26
<210> 651
<211> 28
<212> DNA
<213> Artifical
<400> 651
cacaattaca tttaatatgc caaagggc 28
<210> 652
<211> 19
<212> DNA
<213> Artifical
<400> 652
cggcaggtca cctctcctc 19
<210> 653
<211> 26
<212> DNA
<213> Artifical
<400> 653
aggtttctag tttcactggt ctcaaa 26
<210> 654
<211> 26
<212> DNA
<213> Artifical
<400> 654
taaagcagct tattgtaggt gttggc 26
<210> 655
<211> 29
<212> DNA
<213> Artifical
<400> 655
tctctgaatt cacttttcat aaaaacgta 29
<210> 656
<211> 26
<212> DNA
<213> Artifical
<400> 656
ttactctgag tagaccctag gtgaga 26
<210> 657
<211> 26
<212> DNA
<213> Artifical
<400> 657
aacagatact ggatggaaac tggaat 26
<210> 658
<211> 26
<212> DNA
<213> Artifical
<400> 658
tttcaaacgc tatctcctgg acttac 26
<210> 659
<211> 26
<212> DNA
<213> Artifical
<400> 659
attcactgat acctggaagt agtcgc 26
<210> 660
<211> 22
<212> DNA
<213> Artifical
<400> 660
aaacatgtat tttggggtga tg 22
<210> 661
<211> 23
<212> DNA
<213> Artifical
<400> 661
cacttggtgg aagaacagct ttg 23
<210> 662
<211> 26
<212> DNA
<213> Artifical
<400> 662
ttgcatggaa ttgtgaacta atgcta 26
<210> 663
<211> 26
<212> DNA
<213> Artifical
<400> 663
agtgagctct gaaaatgcaa ttcatt 26
<210> 664
<211> 26
<212> DNA
<213> Artifical
<400> 664
ttgtttttac aggaaagggg gaaata 26
<210> 665
<211> 26
<212> DNA
<213> Artifical
<400> 665
acatcccagg tgagttttct atgaaa 26
<210> 666
<211> 26
<212> DNA
<213> Artifical
<400> 666
ttgcaggaaa tccaaatgaa aaggac 26
<210> 667
<211> 26
<212> DNA
<213> Artifical
<400> 667
cagctgagta tcgagaaatt gacaaa 26
<210> 668
<211> 24
<212> DNA
<213> Artifical
<400> 668
gtatgcctag ggaagacagc aagg 24
<210> 669
<211> 26
<212> DNA
<213> Artifical
<400> 669
agttgagact gcacaataat gctttt 26
<210> 670
<211> 26
<212> DNA
<213> Artifical
<400> 670
gtcacactag cctacccagt atatgc 26
<210> 671
<211> 22
<212> DNA
<213> Artifical
<400> 671
atgagagaag ccaagccact gg 22
<210> 672
<211> 26
<212> DNA
<213> Artifical
<400> 672
gccataggac cctcagttct aaatat 26
<210> 673
<211> 22
<212> DNA
<213> Artifical
<400> 673
ggtggctata gaggtaggag cg 22
<210> 674
<211> 26
<212> DNA
<213> Artifical
<400> 674
agcatctata gagcattcat cctctt 26
<210> 675
<211> 26
<212> DNA
<213> Artifical
<400> 675
gcctcttacc cacagatgtt agattc 26
<210> 676
<211> 24
<212> DNA
<213> Artifical
<400> 676
caaacatctt ctcttctgtg gtgt 24
<210> 677
<211> 21
<212> DNA
<213> Artifical
<400> 677
gaagacacct tcctgcaggc g 21
<210> 678
<211> 26
<212> DNA
<213> Artifical
<400> 678
actcctttga agttaaggta cactta 26
<210> 679
<211> 28
<212> DNA
<213> Artifical
<400> 679
ttcaaaatgg gtaataagtc aatgagaa 28
<210> 680
<211> 26
<212> DNA
<213> Artifical
<400> 680
tttccccaag taattggcta tgagtg 26
<210> 681
<211> 26
<212> DNA
<213> Artifical
<400> 681
ttactgcttc tttaggttgt ctacat 26
<210> 682
<211> 26
<212> DNA
<213> Artifical
<400> 682
ctcaaaaact ggaacacttc ttccag 26
<210> 683
<211> 26
<212> DNA
<213> Artifical
<400> 683
aaaggcagtg ttaaaaggat gctttg 26
<210> 684
<211> 27
<212> DNA
<213> Artifical
<400> 684
agctaggaat aaaaagtagt tgaccaa 27
<210> 685
<211> 26
<212> DNA
<213> Artifical
<400> 685
gttgtttcat ccttggaaaa cttgcc 26
<210> 686
<211> 26
<212> DNA
<213> Artifical
<400> 686
tactttctct atgcagattg ccagac 26
<210> 687
<211> 26
<212> DNA
<213> Artifical
<400> 687
tctgtctttc cctttcatgc tatcaa 26
<210> 688
<211> 30
<212> DNA
<213> Artifical
<400> 688
gtctatttac ttcttgatct aacacaggat 30
<210> 689
<211> 26
<212> DNA
<213> Artifical
<400> 689
agcaggtcat ttaaagtcca acaagt 26
<210> 690
<211> 26
<212> DNA
<213> Artifical
<400> 690
agtgctcctt atctacatcc acatcc 26
<210> 691
<211> 24
<212> DNA
<213> Artifical
<400> 691
cttctccgag ttcatgcttt ctgg 24
<210> 692
<211> 24
<212> DNA
<213> Artifical
<400> 692
gatgacacca cccatttctc cagc 24
<210> 693
<211> 26
<212> DNA
<213> Artifical
<400> 693
cagctttcaa gagacttctt cagtgg 26
<210> 694
<211> 27
<212> DNA
<213> Artifical
<400> 694
tctcaacatt ttctgttaca ggatact 27
<210> 695
<211> 26
<212> DNA
<213> Artifical
<400> 695
attgctctgg taaaccatac agactc 26
<210> 696
<211> 26
<212> DNA
<213> Artifical
<400> 696
caattccttc ttcctcatcc caatgt 26
<210> 697
<211> 26
<212> DNA
<213> Artifical
<400> 697
ctggttttgg catgaatgtt taacaa 26
<210> 698
<211> 25
<212> DNA
<213> Artifical
<400> 698
gatgggagaa tcagagatgt gctgc 25
<210> 699
<211> 21
<212> DNA
<213> Artifical
<400> 699
gagagctgtg aggcctcagg g 21
<210> 700
<211> 26
<212> DNA
<213> Artifical
<400> 700
gtgattctca agtgctactg aaagca 26
<210> 701
<211> 20
<212> DNA
<213> Artifical
<400> 701
cacgtgtggg gagcacttcc 20
<210> 702
<211> 25
<212> DNA
<213> Artifical
<400> 702
ctacgaaatc ttggagcttg ggaac 25
<210> 703
<211> 20
<212> DNA
<213> Artifical
<400> 703
cgccctgaga ccaagtctgc 20
<210> 704
<211> 26
<212> DNA
<213> Artifical
<400> 704
aaaaacaagc ccattatctg caatgg 26
<210> 705
<211> 26
<212> DNA
<213> Artifical
<400> 705
acatcaccgg ttgttaagta gtttgg 26
<210> 706
<211> 23
<212> DNA
<213> Artifical
<400> 706
ctcacccctc tccgtttgat agc 23
<210> 707
<211> 26
<212> DNA
<213> Artifical
<400> 707
tttgaatcca gatctccctt ctgagc 26
<210> 708
<211> 20
<212> DNA
<213> Artifical
<400> 708
cctcccgtca ggactagctg 20
<210> 709
<211> 20
<212> DNA
<213> Artifical
<400> 709
tggctgtgag tctgggagtg 20
<210> 710
<211> 20
<212> DNA
<213> Artifical
<400> 710
aggaggtgag aagcctggag 20
<210> 711
<211> 26
<212> DNA
<213> Artifical
<400> 711
cagccttagt tttggtaatc tgcaaa 26
<210> 712
<211> 23
<212> DNA
<213> Artifical
<400> 712
gaaggagcca cagtctgtat ggg 23
<210> 713
<211> 25
<212> DNA
<213> Artifical
<400> 713
ctttgatgac ccccaatgga gatcc 25
<210> 714
<211> 24
<212> DNA
<213> Artifical
<400> 714
cagtgatgcc tcctgtagag aacg 24
<210> 715
<211> 26
<212> DNA
<213> Artifical
<400> 715
gatggataaa tggatggacg aatgga 26
<210> 716
<211> 26
<212> DNA
<213> Artifical
<400> 716
gatggataaa tggatggatg aatgga 26
<210> 717
<211> 26
<212> DNA
<213> Artifical
<400> 717
acaaatttgc ttccaatttc tcacct 26
<210> 718
<211> 26
<212> DNA
<213> Artifical
<400> 718
ggaagataac agtttgtggt ggtttg 26
<210> 719
<211> 22
<212> DNA
<213> Artifical
<400> 719
gacaactcca agacccacct gg 22
<210> 720
<211> 22
<212> DNA
<213> Artifical
<400> 720
acaaagctgg cgttggattt cc 22
<210> 721
<211> 24
<212> DNA
<213> Artifical
<400> 721
gtgagacaga ggtatccaca cacc 24
<210> 722
<211> 23
<212> DNA
<213> Artifical
<400> 722
ctctctcatg tcctctgtct gct 23
<210> 723
<211> 24
<212> DNA
<213> Artifical
<400> 723
ccttcagtag gtccctggag atac 24
<210> 724
<211> 26
<212> DNA
<213> Artifical
<400> 724
tgtagataag ctcttccccc tagaac 26
<210> 725
<211> 19
<212> DNA
<213> Artifical
<400> 725
caggagggag gctgtgtgg 19
<210> 726
<211> 26
<212> DNA
<213> Artifical
<400> 726
tggtgcagca ctattctagt aagagg 26
<210> 727
<211> 22
<212> DNA
<213> Artifical
<400> 727
cccagccctg tcttttcttc cc 22
<210> 728
<211> 26
<212> DNA
<213> Artifical
<400> 728
cttaccccga ttttggttaa gggatc 26
<210> 729
<211> 25
<212> DNA
<213> Artifical
<400> 729
cagtaggtag atccttcctg atgca 25
<210> 730
<211> 19
<212> DNA
<213> Artifical
<400> 730
tgcgggggac acagtgtgg 19
<210> 731
<211> 22
<212> DNA
<213> Artifical
<400> 731
cttgatggaa ggacagcagg ag 22
<210> 732
<211> 26
<212> DNA
<213> Artifical
<400> 732
gtactcagga gaaaatctac agcctc 26
<210> 733
<211> 22
<212> DNA
<213> Artifical
<400> 733
ggtgctggag gacttctaca cg 22
<210> 734
<211> 26
<212> DNA
<213> Artifical
<400> 734
atattatcgt tgttgccgtc gttttc 26
<210> 735
<211> 19
<212> DNA
<213> Artifical
<400> 735
gacggagccg cttacaccc 19
<210> 736
<211> 24
<212> DNA
<213> Artifical
<400> 736
gcgctctaga ttctcagatg tgag 24
<210> 737
<211> 24
<212> DNA
<213> Artifical
<400> 737
gagatctcat gcagggtcgt tagg 24
<210> 738
<211> 23
<212> DNA
<213> Artifical
<400> 738
gcagcaggtg tgtgtctttc aat 23
<210> 739
<211> 23
<212> DNA
<213> Artifical
<400> 739
gtgtgctgac tctgcttcat tcc 23
<210> 740
<211> 20
<212> DNA
<213> Artifical
<400> 740
cgtctccatg ggcctcatcg 20
<210> 741
<211> 24
<212> DNA
<213> Artifical
<400> 741
ctgagagctc atgaacaagc atgt 24
<210> 742
<211> 20
<212> DNA
<213> Artifical
<400> 742
cgccgtgtac acagaaggtg 20
<210> 743
<211> 19
<212> DNA
<213> Artifical
<400> 743
ctgctgcaca gggacaggg 19
<210> 744
<211> 19
<212> DNA
<213> Artifical
<400> 744
gggcatttcc accccacct 19
<210> 745
<211> 24
<212> DNA
<213> Artifical
<400> 745
tagaccctct ccccctacaa caag 24
<210> 746
<211> 25
<212> DNA
<213> Artifical
<400> 746
cagtgtctga aggttactga tcccc 25
<210> 747
<211> 22
<212> DNA
<213> Artifical
<400> 747
gacatgcctg ccatcaacaa gg 22
<210> 748
<211> 26
<212> DNA
<213> Artifical
<400> 748
aataagaaga agactgtggt ggactt 26
<210> 749
<211> 24
<212> DNA
<213> Artifical
<400> 749
aagacctacg cctacctgtt ttcc 24
<210> 750
<211> 18
<212> DNA
<213> Artifical
<400> 750
tcaggcgtgc aggtggag 18
<210> 751
<211> 19
<212> DNA
<213> Artifical
<400> 751
acgggtgact ctgaggctg 19
<210> 752
<211> 19
<212> DNA
<213> Artifical
<400> 752
agactcggtg atgcgaagg 19
<210> 753
<211> 24
<212> DNA
<213> Artifical
<400> 753
ggaggagact atggcaaagt tatc 24
<210> 754
<211> 18
<212> DNA
<213> Artifical
<400> 754
cctcaggtga ctcatgct 18
<210> 755
<211> 19
<212> DNA
<213> Artifical
<400> 755
ttaggtgggg ccatgggtg 19
<210> 756
<211> 24
<212> DNA
<213> Artifical
<400> 756
atctcatcct gaagttgctt cttg 24
<210> 757
<211> 20
<212> DNA
<213> Artifical
<400> 757
agcaagcacc ttggcagaat 20
<210> 758
<211> 20
<212> DNA
<213> Artifical
<400> 758
ggtatcacct gcttctggag 20
<210> 759
<211> 20
<212> DNA
<213> Artifical
<400> 759
atctccatgt ccagggccag 20
<210> 760
<211> 21
<212> DNA
<213> Artifical
<400> 760
tctctccaca tgccatcctt c 21
<210> 761
<211> 20
<212> DNA
<213> Artifical
<400> 761
attggcaaac ttgggttggg 20
<210> 762
<211> 18
<212> DNA
<213> Artifical
<400> 762
tagctgcgag tgaccgtg 18
<210> 763
<211> 24
<212> DNA
<213> Artifical
<400> 763
ggttattttt ctttggcttc aagc 24
<210> 764
<211> 24
<212> DNA
<213> Artifical
<400> 764
tataagtggt gatgaggatc gcaa 24
<210> 765
<211> 24
<212> DNA
<213> Artifical
<400> 765
aacatcaggg gaaaatgaga aagg 24
<210> 766
<211> 22
<212> DNA
<213> Artifical
<400> 766
attttgggag gtgcatccag tg 22
<210> 767
<211> 21
<212> DNA
<213> Artifical
<400> 767
ctctttctac ggaaggccta c 21
<210> 768
<211> 21
<212> DNA
<213> Artifical
<400> 768
ccccttgatt ggctagagga g 21
<210> 769
<211> 19
<212> DNA
<213> Artifical
<400> 769
gccctacctg agagactgt 19
<210> 770
<211> 20
<212> DNA
<213> Artifical
<400> 770
gctattctgg agctcctagc 20
<210> 771
<211> 24
<212> DNA
<213> Artifical
<400> 771
ttgacctata aagagaaatg ccct 24
<210> 772
<211> 22
<212> DNA
<213> Artifical
<400> 772
tggctgggga cgttaatcat ta 22
<210> 773
<211> 22
<212> DNA
<213> Artifical
<400> 773
atgcccaggc tgaagaagat ga 22
<210> 774
<211> 24
<212> DNA
<213> Artifical
<400> 774
ccaccactta agtaggcaga aaaa 24
<210> 775
<211> 23
<212> DNA
<213> Artifical
<400> 775
ttttcagaaa aggccaccac atg 23
<210> 776
<211> 23
<212> DNA
<213> Artifical
<400> 776
agccaagcct atttgttgaa caa 23
<210> 777
<211> 17
<212> DNA
<213> Artifical
<400> 777
gatgaggcag gtccgga 17
<210> 778
<211> 24
<212> DNA
<213> Artifical
<400> 778
aagtgaaact ttaggccttt cctc 24
<210> 779
<211> 20
<212> DNA
<213> Artifical
<400> 779
tgtgtctccc tgactgtgtc 20
<210> 780
<211> 18
<212> DNA
<213> Artifical
<400> 780
tcgtgaagca tgtggggg 18
<210> 781
<211> 22
<212> DNA
<213> Artifical
<400> 781
acgttactct gtggactact cc 22
<210> 782
<211> 22
<212> DNA
<213> Artifical
<400> 782
tgagaccctc atcttcagca ag 22
<210> 783
<211> 17
<212> DNA
<213> Artifical
<400> 783
gcttcctgca ggacgtg 17
<210> 784
<211> 24
<212> DNA
<213> Artifical
<400> 784
cttgagttcg ataagccaga gaag 24
<210> 785
<211> 18
<212> DNA
<213> Artifical
<400> 785
agacctgggc tccacatc 18
<210> 786
<211> 24
<212> DNA
<213> Artifical
<400> 786
atttgccccc atgcacacat tttc 24
<210> 787
<211> 24
<212> DNA
<213> Artifical
<400> 787
tcatcattga tggcattgac atcg 24
<210> 788
<211> 24
<212> DNA
<213> Artifical
<400> 788
cagaaaacca ggccaaaaag atac 24
<210> 789
<211> 21
<212> DNA
<213> Artifical
<400> 789
ctttcggata ctagctgtgg c 21
<210> 790
<211> 23
<212> DNA
<213> Artifical
<400> 790
tccagcctta agaagagcca ttc 23
<210> 791
<211> 22
<212> DNA
<213> Artifical
<400> 791
cgaaaaggag tgaggtagaa ca 22
<210> 792
<211> 17
<212> DNA
<213> Artifical
<400> 792
agggacctgc agcagct 17
<210> 793
<211> 17
<212> DNA
<213> Artifical
<400> 793
gcccaggaca gcagtaa 17
<210> 794
<211> 17
<212> DNA
<213> Artifical
<400> 794
ctccccctcg tctccct 17
<210> 795
<211> 24
<212> DNA
<213> Artifical
<400> 795
catgaatgac tccagagaca ctta 24
<210> 796
<211> 20
<212> DNA
<213> Artifical
<400> 796
tcacgtctgt cactgtggag 20
<210> 797
<211> 17
<212> DNA
<213> Artifical
<400> 797
cagagtgggt tcttgcg 17
<210> 798
<211> 24
<212> DNA
<213> Artifical
<400> 798
atgaggaaga ggaggaaggt acag 24
<210> 799
<211> 17
<212> DNA
<213> Artifical
<400> 799
accattcctc cccagcc 17
<210> 800
<211> 22
<212> DNA
<213> Artifical
<400> 800
tgcctttccc taccctctac tc 22
<210> 801
<211> 20
<212> DNA
<213> Artifical
<400> 801
acagtgtctc ctgaatggct 20
<210> 802
<211> 24
<212> DNA
<213> Artifical
<400> 802
atagctaccc agagactaat gacc 24
<210> 803
<211> 23
<212> DNA
<213> Artifical
<400> 803
gattgtaaag gcatgctcat gtt 23
<210> 804
<211> 24
<212> DNA
<213> Artifical
<400> 804
tgtctgtctg tctttctggg taat 24
<210> 805
<211> 18
<212> DNA
<213> Artifical
<400> 805
ttttcccagg aagagggg 18
<210> 806
<211> 24
<212> DNA
<213> Artifical
<400> 806
actatacttc aggcctgaga tcct 24
<210> 807
<211> 24
<212> DNA
<213> Artifical
<400> 807
gtgtctccaa ctaaaagatc ctct 24
<210> 808
<211> 20
<212> DNA
<213> Artifical
<400> 808
aagcctctcc cctctgactc 20
<210> 809
<211> 17
<212> DNA
<213> Artifical
<400> 809
acctcctggc agggcaa 17
<210> 810
<211> 24
<212> DNA
<213> Artifical
<400> 810
gatgatgaat gtgaaacact ctgc 24
<210> 811
<211> 24
<212> DNA
<213> Artifical
<400> 811
tgaacttctc tttccaacag acac 24
<210> 812
<211> 22
<212> DNA
<213> Artifical
<400> 812
aaggacattc agggcactca ag 22
<210> 813
<211> 19
<212> DNA
<213> Artifical
<400> 813
aagaagccca tcgacttgc 19
<210> 814
<211> 24
<212> DNA
<213> Artifical
<400> 814
catctgttag agatcaagtg tgga 24
<210> 815
<211> 24
<212> DNA
<213> Artifical
<400> 815
tccacttact cggaaatgta caca 24
<210> 816
<211> 24
<212> DNA
<213> Artifical
<400> 816
taaaggttca gtgttcagcc tttg 24
<210> 817
<211> 21
<212> DNA
<213> Artifical
<400> 817
agcacctttc tggggttcat g 21
<210> 818
<211> 21
<212> DNA
<213> Artifical
<400> 818
gcacgtcttc ctactcttca t 21
<210> 819
<211> 17
<212> DNA
<213> Artifical
<400> 819
atctggaagg tgggcgc 17
<210> 820
<211> 24
<212> DNA
<213> Artifical
<400> 820
gtctagtttc tgaaggtgct actt 24
<210> 821
<211> 24
<212> DNA
<213> Artifical
<400> 821
acgtgaaggc atctttaatg atca 24
<210> 822
<211> 22
<212> DNA
<213> Artifical
<400> 822
tagacacagt gctaacctgt cc 22
<210> 823
<211> 24
<212> DNA
<213> Artifical
<400> 823
gagaaagact agcagccaaa atag 24
<210> 824
<211> 24
<212> DNA
<213> Artifical
<400> 824
gcattttaaa gggaattggt tgga 24
<210> 825
<211> 25
<212> DNA
<213> Artifical
<400> 825
ttaccatcct attctttttg ttcca 25
<210> 826
<211> 24
<212> DNA
<213> Artifical
<400> 826
attttggttt gatttgcagg tgtc 24
<210> 827
<211> 22
<212> DNA
<213> Artifical
<400> 827
ggtattacga gactggctcc at 22
<210> 828
<211> 23
<212> DNA
<213> Artifical
<400> 828
acttcgtttt gatgcatctt cag 23
<210> 829
<211> 19
<212> DNA
<213> Artifical
<400> 829
tccctttcct caggtcaca 19
<210> 830
<211> 24
<212> DNA
<213> Artifical
<400> 830
gtggatcaat tcctaaagaa cgga 24
<210> 831
<211> 20
<212> DNA
<213> Artifical
<400> 831
tctttgctca gccctgactc 20
<210> 832
<211> 20
<212> DNA
<213> Artifical
<400> 832
ttcccagcaa ctaggctaaa 20
<210> 833
<211> 24
<212> DNA
<213> Artifical
<400> 833
ctttccatct acctgtctgt gtaa 24
<210> 834
<211> 24
<212> DNA
<213> Artifical
<400> 834
gggcttaggt tcaagtattc tcaa 24
<210> 835
<211> 19
<212> DNA
<213> Artifical
<400> 835
agaggtttag gtgactgct 19
<210> 836
<211> 18
<212> DNA
<213> Artifical
<400> 836
gtcatgaccc caggaagt 18
<210> 837
<211> 17
<212> DNA
<213> Artifical
<400> 837
tgcagctgag ccatggt 17
<210> 838
<211> 22
<212> DNA
<213> Artifical
<400> 838
tgtcggtgat gagcatagtc tg 22
<210> 839
<211> 19
<212> DNA
<213> Artifical
<400> 839
cagtgtctga ggtgaagac 19
<210> 840
<211> 24
<212> DNA
<213> Artifical
<400> 840
ccactgcctc taacaattac ttca 24
<210> 841
<211> 24
<212> DNA
<213> Artifical
<400> 841
aagtaagaag actgtatccc acga 24
<210> 842
<211> 20
<212> DNA
<213> Artifical
<400> 842
atgggtcctt gtaaagctgc 20
<210> 843
<211> 20
<212> DNA
<213> Artifical
<400> 843
gagaaccgga aaggagaaag 20
<210> 844
<211> 24
<212> DNA
<213> Artifical
<400> 844
ccgtgagatg tacttgttga atag 24
<210> 845
<211> 19
<212> DNA
<213> Artifical
<400> 845
ctctcatcgt ggttcctgc 19
<210> 846
<211> 21
<212> DNA
<213> Artifical
<400> 846
ctcgattctt ccccagacaa a 21
<210> 847
<211> 24
<212> DNA
<213> Artifical
<400> 847
tccatctatt tcgctcagaa tccc 24
<210> 848
<211> 24
<212> DNA
<213> Artifical
<400> 848
tgtagctttg atctcttcca gcta 24
<210> 849
<211> 24
<212> DNA
<213> Artifical
<400> 849
taacaggatt ctttgatgtc tgcc 24
<210> 850
<211> 24
<212> DNA
<213> Artifical
<400> 850
acctgatttt ctcctgtcta ggtc 24
<210> 851
<211> 24
<212> DNA
<213> Artifical
<400> 851
cgcagctttt aagtcactta tagc 24
<210> 852
<211> 19
<212> DNA
<213> Artifical
<400> 852
agttcacttg tgcccacag 19
<210> 853
<211> 24
<212> DNA
<213> Artifical
<400> 853
ctaaaaggaa atgtgagaca agga 24
<210> 854
<211> 28
<212> DNA
<213> Artifical
<400> 854
ctttgaaaca gagttgatta aaaatagc 28
<210> 855
<211> 24
<212> DNA
<213> Artifical
<400> 855
ctggatcaga gaggacttct ttag 24
<210> 856
<211> 26
<212> DNA
<213> Artifical
<400> 856
aattaagaag agcagaaaaa cagttg 26
<210> 857
<211> 17
<212> DNA
<213> Artifical
<400> 857
agaagccaaa gcgcagg 17
<210> 858
<211> 21
<212> DNA
<213> Artifical
<400> 858
tccatggaga agctcagagt g 21
<210> 859
<211> 24
<212> DNA
<213> Artifical
<400> 859
gtattctcat gctttcccct ctag 24
<210> 860
<211> 20
<212> DNA
<213> Artifical
<400> 860
aaaagtctgc tggggctgag 20
<210> 861
<211> 23
<212> DNA
<213> Artifical
<400> 861
tactttccag tcctagacat gac 23
<210> 862
<211> 18
<212> DNA
<213> Artifical
<400> 862
atgccttcct gggagctg 18
<210> 863
<211> 22
<212> DNA
<213> Artifical
<400> 863
agaagaccta caccagcact aa 22
<210> 864
<211> 21
<212> DNA
<213> Artifical
<400> 864
atgagaggta gggaagtgac c 21
<210> 865
<211> 19
<212> DNA
<213> Artifical
<400> 865
gggctctgta cctgtgtct 19
<210> 866
<211> 20
<212> DNA
<213> Artifical
<400> 866
aagcacagag tgtccctgtc 20
<210> 867
<211> 18
<212> DNA
<213> Artifical
<400> 867
tggtccaagt ccacccat 18
<210> 868
<211> 24
<212> DNA
<213> Artifical
<400> 868
tactgggttt gtgttgtttt gcag 24
<210> 869
<211> 21
<212> DNA
<213> Artifical
<400> 869
tccaacttgg tcactgaggt c 21
<210> 870
<211> 20
<212> DNA
<213> Artifical
<400> 870
tcagtcaaca gagccatggg 20
<210> 871
<211> 24
<212> DNA
<213> Artifical
<400> 871
gctgctaaaa tgatcaaggg ttac 24
<210> 872
<211> 24
<212> DNA
<213> Artifical
<400> 872
tatgacacac ctcccatcct caag 24
<210> 873
<211> 24
<212> DNA
<213> Artifical
<400> 873
agaagttttc tgactccttt cgga 24
<210> 874
<211> 20
<212> DNA
<213> Artifical
<400> 874
gaagcgtagg aactgagcag 20
<210> 875
<211> 17
<212> DNA
<213> Artifical
<400> 875
tggtgggcac gtagacc 17
<210> 876
<211> 18
<212> DNA
<213> Artifical
<400> 876
gggagagtag gggaagcg 18
<210> 877
<211> 18
<212> DNA
<213> Artifical
<400> 877
ggtcctccag caggtctg 18
<210> 878
<211> 18
<212> DNA
<213> Artifical
<400> 878
tctgcggctt gatgaggg 18
<210> 879
<211> 26
<212> DNA
<213> Artifical
<400> 879
ctgatatcac atacaaacca gatact 26
<210> 880
<211> 24
<212> DNA
<213> Artifical
<400> 880
cctgcaacag tcaaaataaa cctt 24
<210> 881
<211> 23
<212> DNA
<213> Artifical
<400> 881
tcttgacccg aatacttgag ctc 23
<210> 882
<211> 24
<212> DNA
<213> Artifical
<400> 882
tggagtttga ggacatggag aatg 24
<210> 883
<211> 24
<212> DNA
<213> Artifical
<400> 883
atttctgcct tgacaattgt ccac 24
<210> 884
<211> 27
<212> DNA
<213> Artifical
<400> 884
taaatggctt ctttgttact actatca 27
<210> 885
<211> 20
<212> DNA
<213> Artifical
<400> 885
tgctaggacc aaggctgaag 20
<210> 886
<211> 22
<212> DNA
<213> Artifical
<400> 886
caaccccgtg tttctgtttt ag 22
<210> 887
<211> 22
<212> DNA
<213> Artifical
<400> 887
ggtatgtctg ctctcctaga cc 22
<210> 888
<211> 22
<212> DNA
<213> Artifical
<400> 888
aggtgtctcg agagaagatc ac 22
<210> 889
<211> 24
<212> DNA
<213> Artifical
<400> 889
tctaggatta tggggattct gctg 24
<210> 890
<211> 24
<212> DNA
<213> Artifical
<400> 890
ggagtggatg tgatttttga tgtg 24
<210> 891
<211> 22
<212> DNA
<213> Artifical
<400> 891
tcatttctga gcaggatcct gg 22
<210> 892
<211> 24
<212> DNA
<213> Artifical
<400> 892
gacgcatgaa atctttgaga acaa 24
<210> 893
<211> 17
<212> DNA
<213> Artifical
<400> 893
ttcctcgacc agcgtgc 17
<210> 894
<211> 19
<212> DNA
<213> Artifical
<400> 894
tccaagtgtc agagcccag 19
<210> 895
<211> 18
<212> DNA
<213> Artifical
<400> 895
cagccgcaga gacttgag 18
<210> 896
<211> 24
<212> DNA
<213> Artifical
<400> 896
agtgtctaac tcatcatccc agat 24
<210> 897
<211> 19
<212> DNA
<213> Artifical
<400> 897
tagacattga cccacccct 19
<210> 898
<211> 24
<212> DNA
<213> Artifical
<400> 898
ctttggtctg aaactacact gaaa 24
<210> 899
<211> 23
<212> DNA
<213> Artifical
<400> 899
cttccactat aaccccaaac tct 23
<210> 900
<211> 23
<212> DNA
<213> Artifical
<400> 900
tccttcctgg ccatgaaaac ttc 23
<210> 901
<211> 28
<212> DNA
<213> Artifical
<400> 901
gaagattgaa atatgtctat agaaaccc 28
<210> 902
<211> 24
<212> DNA
<213> Artifical
<400> 902
cctttctctt tctctctctc tctg 24
<210> 903
<211> 17
<212> DNA
<213> Artifical
<400> 903
tgtgtggaga cctgccc 17
<210> 904
<211> 20
<212> DNA
<213> Artifical
<400> 904
atcgactggt cccgtatcct 20
<210> 905
<211> 22
<212> DNA
<213> Artifical
<400> 905
tggatatccg gaacaacctc ac 22
<210> 906
<211> 19
<212> DNA
<213> Artifical
<400> 906
ctctgatccg aggagaccc 19
<210> 907
<211> 16
<212> DNA
<213> Artifical
<400> 907
gatgtgagcc tggtgg 16
<210> 908
<211> 20
<212> DNA
<213> Artifical
<400> 908
ttccctcagc catcccaaaa 20
<210> 909
<211> 19
<212> DNA
<213> Artifical
<400> 909
actacctccg cttccagga 19
<210> 910
<211> 17
<212> DNA
<213> Artifical
<400> 910
cccctcactc acacttg 17
<210> 911
<211> 24
<212> DNA
<213> Artifical
<400> 911
acgattaccc ttttcccaga tcta 24
<210> 912
<211> 17
<212> DNA
<213> Artifical
<400> 912
ctgaacctga cccggaa 17
<210> 913
<211> 20
<212> DNA
<213> Artifical
<400> 913
cccctttctt cgtgacatgc 20
<210> 914
<211> 18
<212> DNA
<213> Artifical
<400> 914
tttggccagg tgtgggac 18
<210> 915
<211> 17
<212> DNA
<213> Artifical
<400> 915
cctcagtgcc cacccct 17
<210> 916
<211> 17
<212> DNA
<213> Artifical
<400> 916
ggatgcaaga aggcgcc 17
<210> 917
<211> 17
<212> DNA
<213> Artifical
<400> 917
cagggacctg gcccact 17
<210> 918
<211> 24
<212> DNA
<213> Artifical
<400> 918
tcttcgagct catcctcatg gaag 24
<210> 919
<211> 23
<212> DNA
<213> Artifical
<400> 919
cacatcaaga tcactgactt tgg 23
<210> 920
<211> 16
<212> DNA
<213> Artifical
<400> 920
aaggtgaggg caggtg 16
<210> 921
<211> 22
<212> DNA
<213> Artifical
<400> 921
tgtgtgtgtg cagaataagc ag 22
<210> 922
<211> 22
<212> DNA
<213> Artifical
<400> 922
tgttaaaatt tcctcccacg gg 22
<210> 923
<211> 18
<212> DNA
<213> Artifical
<400> 923
ctgggactca cgtgtgtc 18
<210> 924
<211> 18
<212> DNA
<213> Artifical
<400> 924
actttgggct gcaggcta 18
<210> 925
<211> 22
<212> DNA
<213> Artifical
<400> 925
tgggacatca ctcaaccttg tc 22
<210> 926
<211> 20
<212> DNA
<213> Artifical
<400> 926
accagctcct ctcttgtctt 20
<210> 927
<211> 24
<212> DNA
<213> Artifical
<400> 927
aacttggaag agaatgaggg tcaa 24
<210> 928
<211> 17
<212> DNA
<213> Artifical
<400> 928
ggagcacagg gccagag 17
<210> 929
<211> 19
<212> DNA
<213> Artifical
<400> 929
tctctcggga gcacagttc 19
<210> 930
<211> 23
<212> DNA
<213> Artifical
<400> 930
accggatctc aaagtcgacg ttg 23
<210> 931
<211> 17
<212> DNA
<213> Artifical
<400> 931
aatgcgctgc catggcc 17
<210> 932
<211> 20
<212> DNA
<213> Artifical
<400> 932
agcgagcaga tctggatgac 20
<210> 933
<211> 19
<212> DNA
<213> Artifical
<400> 933
tgtggagagg gagtgggaa 19
<210> 934
<211> 18
<212> DNA
<213> Artifical
<400> 934
acgtcctcct tctgctcg 18
<210> 935
<211> 18
<212> DNA
<213> Artifical
<400> 935
ggtctggtcg ttcccatt 18
<210> 936
<211> 23
<212> DNA
<213> Artifical
<400> 936
taggcatcct tcaggcagta cac 23
<210> 937
<211> 24
<212> DNA
<213> Artifical
<400> 937
aaggtgggaa atggagtggg tata 24
<210> 938
<211> 23
<212> DNA
<213> Artifical
<400> 938
acatgctgaa ttgggcacta tac 23
<210> 939
<211> 19
<212> DNA
<213> Artifical
<400> 939
cagtgaggga cagggattg 19
<210> 940
<211> 19
<212> DNA
<213> Artifical
<400> 940
gccagtgaag ccgtatacg 19
<210> 941
<211> 23
<212> DNA
<213> Artifical
<400> 941
ttggttttct cgatcatctg acg 23
<210> 942
<211> 19
<212> DNA
<213> Artifical
<400> 942
tcaccataca ccacctgcg 19
<210> 943
<211> 18
<212> DNA
<213> Artifical
<400> 943
cacacaccgg tcgatgag 18
<210> 944
<211> 21
<212> DNA
<213> Artifical
<400> 944
aaggaggggg ttatttctgg c 21
<210> 945
<211> 17
<212> DNA
<213> Artifical
<400> 945
ttctcagggc cccacag 17
<210> 946
<211> 19
<212> DNA
<213> Artifical
<400> 946
caggatgggc tcgaagatg 19
<210> 947
<211> 21
<212> DNA
<213> Artifical
<400> 947
atggggtggg tgtctaggat c 21
<210> 948
<211> 20
<212> DNA
<213> Artifical
<400> 948
ttggagtcag aggttgggtc 20
<210> 949
<211> 17
<212> DNA
<213> Artifical
<400> 949
aagtccggcg tgtgcat 17
<210> 950
<211> 19
<212> DNA
<213> Artifical
<400> 950
ccaggaagcg caagacaaa 19
<210> 951
<211> 24
<212> DNA
<213> Artifical
<400> 951
gggaatcttc tcacaagttc ttca 24
<210> 952
<211> 17
<212> DNA
<213> Artifical
<400> 952
gcaagcaggg ctgacag 17
<210> 953
<211> 23
<212> DNA
<213> Artifical
<400> 953
agttttggct agaggtgatg aac 23
<210> 954
<211> 24
<212> DNA
<213> Artifical
<400> 954
aactttttat cacagccatc ccag 24
<210> 955
<211> 24
<212> DNA
<213> Artifical
<400> 955
atactttcat caaaaccagc tccc 24
<210> 956
<211> 24
<212> DNA
<213> Artifical
<400> 956
ctattaccac ctatcccaga ttcc 24
<210> 957
<211> 35
<212> DNA
<213> Artifical
<400> 957
gttgtattat tttatgcaat atgttaataa taact 35
<210> 958
<211> 24
<212> DNA
<213> Artifical
<400> 958
aaaaggcttg aaatttcatg ggcc 24
<210> 959
<211> 26
<212> DNA
<213> Artifical
<400> 959
cagcatgctt cagaaataaa agatca 26
<210> 960
<211> 24
<212> DNA
<213> Artifical
<400> 960
gaacttttta tagtgagggg agca 24
<210> 961
<211> 24
<212> DNA
<213> Artifical
<400> 961
gccttactct aaaagcatct cact 24
<210> 962
<211> 27
<212> DNA
<213> Artifical
<400> 962
ataaatgaga aaaacatttg gatgact 27
<210> 963
<211> 24
<212> DNA
<213> Artifical
<400> 963
agtaattctt aggaatgtgg gcct 24
<210> 964
<211> 24
<212> DNA
<213> Artifical
<400> 964
cttcaaaact ctgagaaggg aaaa 24
<210> 965
<211> 26
<212> DNA
<213> Artifical
<400> 965
caggatatgg ggaagtatta atatga 26
<210> 966
<211> 24
<212> DNA
<213> Artifical
<400> 966
acaaaacctc tttcacatgg tcat 24
<210> 967
<211> 24
<212> DNA
<213> Artifical
<400> 967
ttggaaaata ctggagaatg tgcc 24
<210> 968
<211> 17
<212> DNA
<213> Artifical
<400> 968
gcccagtccg ccttacg 17
<210> 969
<211> 17
<212> DNA
<213> Artifical
<400> 969
atgcacggac tgaacgc 17
<210> 970
<211> 24
<212> DNA
<213> Artifical
<400> 970
aaggcgtatc tggcttttga atat 24
<210> 971
<211> 20
<212> DNA
<213> Artifical
<400> 971
aatcctcccc cttggttctg 20
<210> 972
<211> 24
<212> DNA
<213> Artifical
<400> 972
ccattttgta tcattctcct ccca 24
<210> 973
<211> 19
<212> DNA
<213> Artifical
<400> 973
tcacctgcag gagtacatg 19
<210> 974
<211> 24
<212> DNA
<213> Artifical
<400> 974
agtcataaag tgtggctaca gttg 24
<210> 975
<211> 20
<212> DNA
<213> Artifical
<400> 975
ggtgagctcc tctgtagtgt 20
<210> 976
<211> 19
<212> DNA
<213> Artifical
<400> 976
actactgccc accatccac 19
<210> 977
<211> 24
<212> DNA
<213> Artifical
<400> 977
gaaagagaga aggaacagtg tttg 24
<210> 978
<211> 22
<212> DNA
<213> Artifical
<400> 978
agagagtcag ggatggctta gg 22
<210> 979
<211> 24
<212> DNA
<213> Artifical
<400> 979
ttgatgaact ggatctgctc gatc 24
<210> 980
<211> 23
<212> DNA
<213> Artifical
<400> 980
gtgcctgaac aaatagacca ttg 23
<210> 981
<211> 24
<212> DNA
<213> Artifical
<400> 981
tttgcaaggt aaaatcccag agtt 24
<210> 982
<211> 20
<212> DNA
<213> Artifical
<400> 982
gctggaagga caaacagaca 20
<210> 983
<211> 24
<212> DNA
<213> Artifical
<400> 983
ctccccaagt caaagtcttg ttat 24
<210> 984
<211> 23
<212> DNA
<213> Artifical
<400> 984
cactggatct gttcttgtga atg 23
<210> 985
<211> 24
<212> DNA
<213> Artifical
<400> 985
tcatgaggct tattgtagag ctga 24
<210> 986
<211> 24
<212> DNA
<213> Artifical
<400> 986
cactttctta ggctttcaaa tctt 24
<210> 987
<211> 24
<212> DNA
<213> Artifical
<400> 987
atttttggca atggctttag tgtc 24
<210> 988
<211> 24
<212> DNA
<213> Artifical
<400> 988
gcaatatgtt aacagccaac agtt 24
<210> 989
<211> 24
<212> DNA
<213> Artifical
<400> 989
tgtcatctaa ttccagtgca ttga 24
<210> 990
<211> 23
<212> DNA
<213> Artifical
<400> 990
gtcttcttga tcacctgcag tag 23
<210> 991
<211> 18
<212> DNA
<213> Artifical
<400> 991
tgtcctccag cgtctcct 18
<210> 992
<211> 24
<212> DNA
<213> Artifical
<400> 992
aaatagctgt ggcatcttct tcaa 24
<210> 993
<211> 24
<212> DNA
<213> Artifical
<400> 993
gcttcaatcg tctatcactt ttgt 24
<210> 994
<211> 28
<212> DNA
<213> Artifical
<400> 994
gctttattca taaaataaaa gctaagca 28
<210> 995
<211> 24
<212> DNA
<213> Artifical
<400> 995
ttattacaaa gaatctggcc acag 24
<210> 996
<211> 28
<212> DNA
<213> Artifical
<400> 996
cttttaggac agttataatc caaataca 28
<210> 997
<211> 24
<212> DNA
<213> Artifical
<400> 997
gtcattgagc tgttatcatt caca 24
<210> 998
<211> 24
<212> DNA
<213> Artifical
<400> 998
catggtctct atatcactgt ccat 24
<210> 999
<211> 24
<212> DNA
<213> Artifical
<400> 999
ccaagcctgt tttactgaat gaag 24
<210> 1000
<211> 27
<212> DNA
<213> Artifical
<400> 1000
gtccataaaa tttctaaaca aaaagga 27
<210> 1001
<211> 26
<212> DNA
<213> Artifical
<400> 1001
ctactcattc aaggtcaaat taaaaa 26
<210> 1002
<211> 24
<212> DNA
<213> Artifical
<400> 1002
cataaaacaa gaaggcattg catt 24
<210> 1003
<211> 24
<212> DNA
<213> Artifical
<400> 1003
gcatggttct tccctaacaa tatg 24
<210> 1004
<211> 24
<212> DNA
<213> Artifical
<400> 1004
cgattctgtc atacgaaaga tgtt 24
<210> 1005
<211> 24
<212> DNA
<213> Artifical
<400> 1005
acttaactac aacagccaaa gacc 24
<210> 1006
<211> 24
<212> DNA
<213> Artifical
<400> 1006
gtaacaaaat tcacatgtgc ctca 24
<210> 1007
<211> 22
<212> DNA
<213> Artifical
<400> 1007
aactgtcaaa ttccaagggg ga 22
<210> 1008
<211> 17
<212> DNA
<213> Artifical
<400> 1008
ggccgctgcg tctctaa 17
<210> 1009
<211> 19
<212> DNA
<213> Artifical
<400> 1009
ttctctgtgg gctgtggag 19
<210> 1010
<211> 24
<212> DNA
<213> Artifical
<400> 1010
cagcgttagg tttggtaaga tttt 24
<210> 1011
<211> 22
<212> DNA
<213> Artifical
<400> 1011
tctctacagg aaggttctgg tc 22
<210> 1012
<211> 17
<212> DNA
<213> Artifical
<400> 1012
gagtccttgg gaggcag 17
<210> 1013
<211> 24
<212> DNA
<213> Artifical
<400> 1013
atcaggtact ccttgatctc catg 24
<210> 1014
<211> 22
<212> DNA
<213> Artifical
<400> 1014
gtagctggtc acggtaaaga ag 22
<210> 1015
<211> 19
<212> DNA
<213> Artifical
<400> 1015
gctccagaga cgtgaacca 19
<210> 1016
<211> 20
<212> DNA
<213> Artifical
<400> 1016
gaagaacggg agcatgttga 20
<210> 1017
<211> 20
<212> DNA
<213> Artifical
<400> 1017
atggccttga gctcgaagac 20
<210> 1018
<211> 24
<212> DNA
<213> Artifical
<400> 1018
tattcagagt ggacagagct acac 24
<210> 1019
<211> 24
<212> DNA
<213> Artifical
<400> 1019
aactagcact cttaacacaa aggc 24
<210> 1020
<211> 24
<212> DNA
<213> Artifical
<400> 1020
tccaaatgag cagtaaaaca caca 24
<210> 1021
<211> 30
<212> DNA
<213> Artifical
<400> 1021
ctttcataag gtcttattaa ttagtaacct 30
<210> 1022
<211> 32
<212> DNA
<213> Artifical
<400> 1022
gagagaaata aaatattaga cttaaatggt ca 32
<210> 1023
<211> 24
<212> DNA
<213> Artifical
<400> 1023
tcccttctgc ttattccatt cctt 24
<210> 1024
<211> 33
<212> DNA
<213> Artifical
<400> 1024
gatttttaaa aggaattgtt tatttagaag agt 33
<210> 1025
<211> 32
<212> DNA
<213> Artifical
<400> 1025
ggtttaactt taatcatatt gattatttga gt 32
<210> 1026
<211> 22
<212> DNA
<213> Artifical
<400> 1026
agacaactcc gaggacaaaa ga 22
<210> 1027
<211> 24
<212> DNA
<213> Artifical
<400> 1027
cgtaaacaga catcttaccg tttt 24
<210> 1028
<211> 24
<212> DNA
<213> Artifical
<400> 1028
ggaagaaata attgttcaac cagt 24
<210> 1029
<211> 24
<212> DNA
<213> Artifical
<400> 1029
ggaagaaata attgttcaac cagt 24
<210> 1030
<211> 24
<212> DNA
<213> Artifical
<400> 1030
tttctttctg agagcatttc acaa 24
<210> 1031
<211> 24
<212> DNA
<213> Artifical
<400> 1031
ggaagaaata atttttcaac cagt 24
<210> 1032
<211> 24
<212> DNA
<213> Artifical
<400> 1032
gactcaaccc aaacttaaga cttt 24
<210> 1033
<211> 24
<212> DNA
<213> Artifical
<400> 1033
tggacatagc tggtagtagg aaat 24
<210> 1034
<211> 24
<212> DNA
<213> Artifical
<400> 1034
agcacctcta catctccatg tatg 24
<210> 1035
<211> 24
<212> DNA
<213> Artifical
<400> 1035
cttcggagct ttgtacttct acaa 24
<210> 1036
<211> 24
<212> DNA
<213> Artifical
<400> 1036
cacatgcatc tctggagtag tata 24
<210> 1037
<211> 24
<212> DNA
<213> Artifical
<400> 1037
agaagctgtg ttacttcaaa ggag 24
<210> 1038
<211> 24
<212> DNA
<213> Artifical
<400> 1038
ggatctttta cagtagcttt cgaa 24
<210> 1039
<211> 24
<212> DNA
<213> Artifical
<400> 1039
cgtctttgga agagaaccaa ctat 24
<210> 1040
<211> 26
<212> DNA
<213> Artifical
<400> 1040
gtttattaat tcaaccacag aactga 26
<210> 1041
<211> 28
<212> DNA
<213> Artifical
<400> 1041
ccatgttagt ctttaaaagt ttagaact 28
<210> 1042
<211> 25
<212> DNA
<213> Artifical
<400> 1042
gaggattttg gttatctcta gcaca 25
<210> 1043
<211> 24
<212> DNA
<213> Artifical
<400> 1043
tctggtttaa tgctgttcat acgt 24
<210> 1044
<211> 24
<212> DNA
<213> Artifical
<400> 1044
cctacaagat gttccaaact cagt 24
<210> 1045
<211> 24
<212> DNA
<213> Artifical
<400> 1045
cccccaaaac tcaatgaatg aata 24
<210> 1046
<211> 21
<212> DNA
<213> Artifical
<400> 1046
caaagagtaa gcgcttcatc g 21
<210> 1047
<211> 20
<212> DNA
<213> Artifical
<400> 1047
cataaatgcc gggtgatcct 20
<210> 1048
<211> 17
<212> DNA
<213> Artifical
<400> 1048
gtggccaggt cccagga 17
<210> 1049
<211> 23
<212> DNA
<213> Artifical
<400> 1049
tacacctgtg gcaaatgttt cag 23
<210> 1050
<211> 24
<212> DNA
<213> Artifical
<400> 1050
cttcttgagt ctctcctgtt cttc 24
<210> 1051
<211> 19
<212> DNA
<213> Artifical
<400> 1051
aaggaatcca gtcaggcct 19
<210> 1052
<211> 21
<212> DNA
<213> Artifical
<400> 1052
acacagcagt cttcctggat c 21
<210> 1053
<211> 24
<212> DNA
<213> Artifical
<400> 1053
cttatgtttg ttcgggatgc atta 24
<210> 1054
<211> 24
<212> DNA
<213> Artifical
<400> 1054
gtattgcaaa tccttcacca gtac 24
<210> 1055
<211> 24
<212> DNA
<213> Artifical
<400> 1055
tgaaggcaaa tgctactgct taaa 24
<210> 1056
<211> 28
<212> DNA
<213> Artifical
<400> 1056
ggaaacatct tgataagcaa aattaaga 28
<210> 1057
<211> 22
<212> DNA
<213> Artifical
<400> 1057
atgaaattcc cagcaaattg ac 22
<210> 1058
<211> 24
<212> DNA
<213> Artifical
<400> 1058
tggcagtgag ttttgtacat catt 24
<210> 1059
<211> 33
<212> DNA
<213> Artifical
<400> 1059
catatttact gaaaatatat tgcaagaatt tag 33
<210> 1060
<211> 22
<212> DNA
<213> Artifical
<400> 1060
ctcgacttag caaccaacat ac 22
<210> 1061
<211> 24
<212> DNA
<213> Artifical
<400> 1061
catagaatta acgacatgct ggtc 24
<210> 1062
<211> 24
<212> DNA
<213> Artifical
<400> 1062
aatgtgggga aacatttttg gttg 24
<210> 1063
<211> 24
<212> DNA
<213> Artifical
<400> 1063
tcctaatttc agtatttggc ctgc 24
<210> 1064
<211> 25
<212> DNA
<213> Artifical
<400> 1064
ccattttctc atagctccat attca 25
<210> 1065
<211> 24
<212> DNA
<213> Artifical
<400> 1065
aagtagtgct ccaatagtgt gact 24
<210> 1066
<211> 24
<212> DNA
<213> Artifical
<400> 1066
tggaaaccat gctcccttta ttac 24
<210> 1067
<211> 24
<212> DNA
<213> Artifical
<400> 1067
attggtcctt ggttaatgac actt 24
<210> 1068
<211> 24
<212> DNA
<213> Artifical
<400> 1068
aactgcatat tgtgtgaatc ctgg 24
<210> 1069
<211> 24
<212> DNA
<213> Artifical
<400> 1069
gactctgggt agtttatgtt gctt 24
<210> 1070
<211> 24
<212> DNA
<213> Artifical
<400> 1070
taaatcaacc gtggaaacat gctt 24
<210> 1071
<211> 24
<212> DNA
<213> Artifical
<400> 1071
tggatactgg ttggaagttc cata 24
<210> 1072
<211> 22
<212> DNA
<213> Artifical
<400> 1072
aaacattctc cactcccaat ga 22
<210> 1073
<211> 19
<212> DNA
<213> Artifical
<400> 1073
cctgaagccg ggatgataa 19
<210> 1074
<211> 19
<212> DNA
<213> Artifical
<400> 1074
caggataagc caggagcag 19
<210> 1075
<211> 27
<212> DNA
<213> Artifical
<400> 1075
gttgtttcct ttgtttttaa gcattca 27
<210> 1076
<211> 24
<212> DNA
<213> Artifical
<400> 1076
ctgttggtga ctataaagga caca 24
<210> 1077
<211> 24
<212> DNA
<213> Artifical
<400> 1077
ccaagatttc gtagtcctct tctc 24
<210> 1078
<211> 20
<212> DNA
<213> Artifical
<400> 1078
catttctcca gagaggggtc 20
<210> 1079
<211> 24
<212> DNA
<213> Artifical
<400> 1079
cgttcctaat ccctgacatt tcta 24
<210> 1080
<211> 21
<212> DNA
<213> Artifical
<400> 1080
gacaaagcag agacaggcat g 21
<210> 1081
<211> 21
<212> DNA
<213> Artifical
<400> 1081
tcaggagcag aagggaacaa t 21
<210> 1082
<211> 24
<212> DNA
<213> Artifical
<400> 1082
acaagcatca gatgaaacac aaga 24
<210> 1083
<211> 23
<212> DNA
<213> Artifical
<400> 1083
tgttgcagtg tccctgagga ata 23
<210> 1084
<211> 24
<212> DNA
<213> Artifical
<400> 1084
tccctcctcc tctttgtaat atcc 24
<210> 1085
<211> 24
<212> DNA
<213> Artifical
<400> 1085
ccagctggtt ttacctgtag taaa 24
<210> 1086
<211> 17
<212> DNA
<213> Artifical
<400> 1086
gctccgcctc tcccact 17
<210> 1087
<211> 20
<212> DNA
<213> Artifical
<400> 1087
gaaccacatt ctcccaggga 20
<210> 1088
<211> 24
<212> DNA
<213> Artifical
<400> 1088
attcaagtgt gtatatgcag ggtg 24
<210> 1089
<211> 21
<212> DNA
<213> Artifical
<400> 1089
ctcatgggct cttccatttc t 21
<210> 1090
<211> 24
<212> DNA
<213> Artifical
<400> 1090
cctagaacct ctttgtatcc aacc 24
<210> 1091
<211> 24
<212> DNA
<213> Artifical
<400> 1091
ttctgagttc aggctaggaa catg 24
<210> 1092
<211> 23
<212> DNA
<213> Artifical
<400> 1092
atctgaccag aggaatcacc atc 23
<210> 1093
<211> 19
<212> DNA
<213> Artifical
<400> 1093
tcttggagtt gtccccagg 19
<210> 1094
<211> 18
<212> DNA
<213> Artifical
<400> 1094
agggcgttac taccgcac 18
<210> 1095
<211> 17
<212> DNA
<213> Artifical
<400> 1095
gggcagtggg agaggtg 17
<210> 1096
<211> 23
<212> DNA
<213> Artifical
<400> 1096
ttgtccggct ttttgctact tac 23
<210> 1097
<211> 24
<212> DNA
<213> Artifical
<400> 1097
tttctttttc ttttcaaggc tgcc 24
<210> 1098
<211> 20
<212> DNA
<213> Artifical
<400> 1098
gatgtggtgg gtggagaaag 20
<210> 1099
<211> 22
<212> DNA
<213> Artifical
<400> 1099
tttcctgtcc cctctttgct ag 22
<210> 1100
<211> 18
<212> DNA
<213> Artifical
<400> 1100
aagtccctgc ccccattg 18
<210> 1101
<211> 24
<212> DNA
<213> Artifical
<400> 1101
agaccatccc ccttctctgc atta 24
<210> 1102
<211> 23
<212> DNA
<213> Artifical
<400> 1102
ctccacttcc tcctctgtaa aat 23
<210> 1103
<211> 22
<212> DNA
<213> Artifical
<400> 1103
tgagaacatg gctctgtcag aa 22
<210> 1104
<211> 23
<212> DNA
<213> Artifical
<400> 1104
ttggggatat ctgcctagag gaa 23
<210> 1105
<211> 24
<212> DNA
<213> Artifical
<400> 1105
tgctgatcta gagctgtaga aacc 24
<210> 1106
<211> 24
<212> DNA
<213> Artifical
<400> 1106
tctgggactc actctgaaaa gaac 24
<210> 1107
<211> 17
<212> DNA
<213> Artifical
<400> 1107
cggaagtcgg ggtcgtc 17
<210> 1108
<211> 20
<212> DNA
<213> Artifical
<400> 1108
aagctctgat acatggtccc 20
<210> 1109
<211> 17
<212> DNA
<213> Artifical
<400> 1109
gtccccggga aggagaa 17
<210> 1110
<211> 22
<212> DNA
<213> Artifical
<400> 1110
aaggcgttgg tggaaaaaca ag 22
<210> 1111
<211> 24
<212> DNA
<213> Artifical
<400> 1111
ccaaaattca aaaccagagg atgt 24
<210> 1112
<211> 24
<212> DNA
<213> Artifical
<400> 1112
gtacaaagga agaagacaag ggag 24
<210> 1113
<211> 17
<212> DNA
<213> Artifical
<400> 1113
ctccccacaa acagccc 17
<210> 1114
<211> 23
<212> DNA
<213> Artifical
<400> 1114
actgactgag aacgtctgaa aag 23
<210> 1115
<211> 24
<212> DNA
<213> Artifical
<400> 1115
caagtttctt cccagttgtt gttc 24
<210> 1116
<211> 24
<212> DNA
<213> Artifical
<400> 1116
ttcttattga cgccttccac gaac 24
<210> 1117
<211> 24
<212> DNA
<213> Artifical
<400> 1117
atttcatgga agttcagctt tgca 24
<210> 1118
<211> 22
<212> DNA
<213> Artifical
<400> 1118
cctcctgcat ctcatgattc ta 22
<210> 1119
<211> 23
<212> DNA
<213> Artifical
<400> 1119
aagagtgggt ttttctggat gac 23
<210> 1120
<211> 20
<212> DNA
<213> Artifical
<400> 1120
agcagctact cacactccag 20
<210> 1121
<211> 23
<212> DNA
<213> Artifical
<400> 1121
ttactccgtg actttcttgt tgc 23
<210> 1122
<211> 17
<212> DNA
<213> Artifical
<400> 1122
caggaaggcc cccagga 17
<210> 1123
<211> 24
<212> DNA
<213> Artifical
<400> 1123
aaaacgtact gaatgtcatc tgca 24
<210> 1124
<211> 20
<212> DNA
<213> Artifical
<400> 1124
tcactgcata ctgggcactg 20
<210> 1125
<211> 17
<212> DNA
<213> Artifical
<400> 1125
gagtggcctc agagtcc 17
<210> 1126
<211> 17
<212> DNA
<213> Artifical
<400> 1126
gagtggcctc ggagtcc 17
<210> 1127
<211> 24
<212> DNA
<213> Artifical
<400> 1127
acaggggtat gaggctttat tcag 24
<210> 1128
<211> 24
<212> DNA
<213> Artifical
<400> 1128
gggcctttgc gtagttgtat atag 24
<210> 1129
<211> 24
<212> DNA
<213> Artifical
<400> 1129
tttcagctcc ttaaactcct cacg 24
<210> 1130
<211> 24
<212> DNA
<213> Artifical
<400> 1130
aaaataacaa gtgggagggg aaga 24
<210> 1131
<211> 24
<212> DNA
<213> Artifical
<400> 1131
agcagggacc aaagtagacc ctag 24
<210> 1132
<211> 22
<212> DNA
<213> Artifical
<400> 1132
tgaaatcagg tgacaggtca ga 22
<210> 1133
<211> 21
<212> DNA
<213> Artifical
<400> 1133
tagggcaagg gactcaggaa g 21
<210> 1134
<211> 22
<212> DNA
<213> Artifical
<400> 1134
aggacaaagg gttaggactg tg 22
<210> 1135
<211> 24
<212> DNA
<213> Artifical
<400> 1135
taagagtgta cagaaacagc acac 24
<210> 1136
<211> 18
<212> DNA
<213> Artifical
<400> 1136
tttttggtga cgctgccc 18
<210> 1137
<211> 24
<212> DNA
<213> Artifical
<400> 1137
tggagaggag aaaaggacac ttac 24
<210> 1138
<211> 24
<212> DNA
<213> Artifical
<400> 1138
taatgaaaag atttttggca cggg 24
<210> 1139
<211> 18
<212> DNA
<213> Artifical
<400> 1139
aagagaaggc aggctctc 18
<210> 1140
<211> 17
<212> DNA
<213> Artifical
<400> 1140
ccctctccct cctccca 17
<210> 1141
<211> 24
<212> DNA
<213> Artifical
<400> 1141
gaaagaattg gtagcataaa gggt 24
<210> 1142
<211> 23
<212> DNA
<213> Artifical
<400> 1142
gaagagcctt ctgattcaga gaa 23
<210> 1143
<211> 22
<212> DNA
<213> Artifical
<400> 1143
aaacacccag gaatccactg aa 22
<210> 1144
<211> 24
<212> DNA
<213> Artifical
<400> 1144
tcaacgcata gacaatttca gtgt 24
<210> 1145
<211> 21
<212> DNA
<213> Artifical
<400> 1145
ttactggacg aaactgctct c 21
<210> 1146
<211> 21
<212> DNA
<213> Artifical
<400> 1146
tctgtaggct tcctccgaat c 21
<210> 1147
<211> 23
<212> DNA
<213> Artifical
<400> 1147
taattcatcc tcctgcgtct agg 23
<210> 1148
<211> 23
<212> DNA
<213> Artifical
<400> 1148
gtggaagcta agttccccta aag 23
<210> 1149
<211> 17
<212> DNA
<213> Artifical
<400> 1149
cggatggggt agggtcc 17
<210> 1150
<211> 24
<212> DNA
<213> Artifical
<400> 1150
atctaaaggc tggaaatgtg tctg 24
<210> 1151
<211> 19
<212> DNA
<213> Artifical
<400> 1151
cgtagatgct gctgctgaa 19
<210> 1152
<211> 17
<212> DNA
<213> Artifical
<400> 1152
gagtggcacc ttcacct 17
<210> 1153
<211> 23
<212> DNA
<213> Artifical
<400> 1153
gtgatgaaga agacgtaggt gac 23
<210> 1154
<211> 20
<212> DNA
<213> Artifical
<400> 1154
cagtgttaag cccaagcaca 20
<210> 1155
<211> 19
<212> DNA
<213> Artifical
<400> 1155
tgttccggaa cctgctctg 19
<210> 1156
<211> 17
<212> DNA
<213> Artifical
<400> 1156
tgtggatgcg cctcctg 17
<210> 1157
<211> 20
<212> DNA
<213> Artifical
<400> 1157
agcctactgg aagctctgac 20
<210> 1158
<211> 19
<212> DNA
<213> Artifical
<400> 1158
cccgctgatc atctaccat 19
<210> 1159
<211> 24
<212> DNA
<213> Artifical
<400> 1159
tccattgagg tccaagtgac tatt 24
<210> 1160
<211> 17
<212> DNA
<213> Artifical
<400> 1160
tgcagtgagg ccctagg 17
<210> 1161
<211> 22
<212> DNA
<213> Artifical
<400> 1161
agagcttgta gtcacatcca cc 22
<210> 1162
<211> 24
<212> DNA
<213> Artifical
<400> 1162
actcacagca caagattaac tcag 24
<210> 1163
<211> 18
<212> DNA
<213> Artifical
<400> 1163
cgctcactgc cctctttg 18
<210> 1164
<211> 24
<212> DNA
<213> Artifical
<400> 1164
acacgttcga aggtgagttg taag 24
<210> 1165
<211> 24
<212> DNA
<213> Artifical
<400> 1165
cagaaaacca ggccaaaaag atac 24
<210> 1166
<211> 21
<212> DNA
<213> Artifical
<400> 1166
aaaaccgagg cagagagcta c 21
<210> 1167
<211> 23
<212> DNA
<213> Artifical
<400> 1167
aaaaggagtg aggtagaaca ggg 23
<210> 1168
<211> 20
<212> DNA
<213> Artifical
<400> 1168
tctgcaggta gggagactcc 20
<210> 1169
<211> 18
<212> DNA
<213> Artifical
<400> 1169
agcagctgga tgacacca 18
<210> 1170
<211> 19
<212> DNA
<213> Artifical
<400> 1170
tgagagcaaa acatggcgg 19
<210> 1171
<211> 22
<212> DNA
<213> Artifical
<400> 1171
ctgctaaacc ggatcatcct ag 22
<210> 1172
<211> 17
<212> DNA
<213> Artifical
<400> 1172
gaccctgacc cctgcag 17
<210> 1173
<211> 24
<212> DNA
<213> Artifical
<400> 1173
aatgggactg ggagtgaaat tatg 24
<210> 1174
<211> 24
<212> DNA
<213> Artifical
<400> 1174
catcaccaga gtagttaccc ctta 24
<210> 1175
<211> 24
<212> DNA
<213> Artifical
<400> 1175
ctaggtccta ggagtatgtt ggtt 24
<210> 1176
<211> 24
<212> DNA
<213> Artifical
<400> 1176
tccctagagc agagttgata gaac 24
<210> 1177
<211> 24
<212> DNA
<213> Artifical
<400> 1177
tttgtttgta ctggtcagat ggtc 24
<210> 1178
<211> 21
<212> DNA
<213> Artifical
<400> 1178
tcagcttgac aactgcctct g 21
<210> 1179
<211> 23
<212> DNA
<213> Artifical
<400> 1179
attgcttctc ccctaagact cag 23
<210> 1180
<211> 24
<212> DNA
<213> Artifical
<400> 1180
gaatcagtgt cttttggctt tcat 24
<210> 1181
<211> 19
<212> DNA
<213> Artifical
<400> 1181
aggggtgtct ctgtgcttc 19
<210> 1182
<211> 17
<212> DNA
<213> Artifical
<400> 1182
caccctggcc tcacttg 17
<210> 1183
<211> 18
<212> DNA
<213> Artifical
<400> 1183
tgcctccaag ccatcctg 18
<210> 1184
<211> 22
<212> DNA
<213> Artifical
<400> 1184
atggcagtga cagtattgac ac 22
<210> 1185
<211> 24
<212> DNA
<213> Artifical
<400> 1185
gctaacctcc ttttgaaatt gctt 24
<210> 1186
<211> 24
<212> DNA
<213> Artifical
<400> 1186
gtgatacagt accaccttac agag 24
<210> 1187
<211> 24
<212> DNA
<213> Artifical
<400> 1187
aataatgatg gccgtggtcc atta 24
<210> 1188
<211> 24
<212> DNA
<213> Artifical
<400> 1188
agttcaccta tttgcagagg atgt 24
<210> 1189
<211> 23
<212> DNA
<213> Artifical
<400> 1189
tacatcttct tcaagacacc gag 23
<210> 1190
<211> 17
<212> DNA
<213> Artifical
<400> 1190
atctctcctc ggcagcc 17
<210> 1191
<211> 24
<212> DNA
<213> Artifical
<400> 1191
atcacaacat cgagacttcc aact 24
<210> 1192
<211> 21
<212> DNA
<213> Artifical
<400> 1192
ttcgtcctgg tgtgtgagat t 21
<210> 1193
<211> 22
<212> DNA
<213> Artifical
<400> 1193
ccatcctctt cattggtgag tg 22
<210> 1194
<211> 18
<212> DNA
<213> Artifical
<400> 1194
agctgcaagg gacagagg 18
<210> 1195
<211> 24
<212> DNA
<213> Artifical
<400> 1195
ttccatgtct gtttctcaaa ggga 24
<210> 1196
<211> 23
<212> DNA
<213> Artifical
<400> 1196
gttcctcagt aaccacaggt ttg 23
<210> 1197
<211> 20
<212> DNA
<213> Artifical
<400> 1197
tttaaacctg tttgctccgg 20
<210> 1198
<211> 24
<212> DNA
<213> Artifical
<400> 1198
ttgtctttcc tagagacaga ggtg 24
<210> 1199
<211> 24
<212> DNA
<213> Artifical
<400> 1199
gacagttacc ttgggaatgt tttg 24
<210> 1200
<211> 24
<212> DNA
<213> Artifical
<400> 1200
tccaactaaa tttccatgcc caaa 24
<210> 1201
<211> 25
<212> DNA
<213> Artifical
<400> 1201
tctctacagt aagttctcat accat 25
<210> 1202
<211> 24
<212> DNA
<213> Artifical
<400> 1202
gaagattgta gagctagctc acag 24
<210> 1203
<211> 24
<212> DNA
<213> Artifical
<400> 1203
cctttatttg atcgatagca ggga 24
<210> 1204
<211> 22
<212> DNA
<213> Artifical
<400> 1204
ctggctcagt ttagaaacca tg 22
<210> 1205
<211> 23
<212> DNA
<213> Artifical
<400> 1205
aggttctcca gctctttgag gat 23
<210> 1206
<211> 20
<212> DNA
<213> Artifical
<400> 1206
ttgcggacgt accaggtgta 20
<210> 1207
<211> 23
<212> DNA
<213> Artifical
<400> 1207
tacctattgc actcctccac tag 23
<210> 1208
<211> 20
<212> DNA
<213> Artifical
<400> 1208
gcaaaccagt tgtagacacg 20
<210> 1209
<211> 24
<212> DNA
<213> Artifical
<400> 1209
atgaggttgg gttttcctta caag 24
<210> 1210
<211> 21
<212> DNA
<213> Artifical
<400> 1210
aatgagtccc agtggctctt c 21
<210> 1211
<211> 23
<212> DNA
<213> Artifical
<400> 1211
acacagatac gtgcattcac atg 23
<210> 1212
<211> 18
<212> DNA
<213> Artifical
<400> 1212
ctggacctta cctgcttg 18
<210> 1213
<211> 19
<212> DNA
<213> Artifical
<400> 1213
ggtgggacca acatggaag 19
<210> 1214
<211> 17
<212> DNA
<213> Artifical
<400> 1214
ctgctgccct catcacc 17
<210> 1215
<211> 17
<212> DNA
<213> Artifical
<400> 1215
tgtacaggca cgcaggg 17
<210> 1216
<211> 19
<212> DNA
<213> Artifical
<400> 1216
acctcgtacg gggagatgt 19
<210> 1217
<211> 17
<212> DNA
<213> Artifical
<400> 1217
gttgggctcc tccagga 17
<210> 1218
<211> 24
<212> DNA
<213> Artifical
<400> 1218
gaaagatgct tcaaactgga ctag 24
<210> 1219
<211> 17
<212> DNA
<213> Artifical
<400> 1219
agaagctcct cgccgga 17
<210> 1220
<211> 23
<212> DNA
<213> Artifical
<400> 1220
aatggctctt tttcttccgg aag 23
<210> 1221
<211> 24
<212> DNA
<213> Artifical
<400> 1221
aaaagagggc cccaatacta caaa 24
<210> 1222
<211> 17
<212> DNA
<213> Artifical
<400> 1222
ccgggagccc acttcct 17
<210> 1223
<211> 24
<212> DNA
<213> Artifical
<400> 1223
tgcccaattc aagatgtttc tcaa 24
<210> 1224
<211> 24
<212> DNA
<213> Artifical
<400> 1224
aaattatctc tccccaagtt ctgt 24
<210> 1225
<211> 24
<212> DNA
<213> Artifical
<400> 1225
aaggtcaaat acgttctctc aaaa 24
<210> 1226
<211> 23
<212> DNA
<213> Artifical
<400> 1226
ctggtcaact gcagtatcta aga 23
<210> 1227
<211> 24
<212> DNA
<213> Artifical
<400> 1227
cacaaacctt gaggaacaaa agaa 24
<210> 1228
<211> 26
<212> DNA
<213> Artifical
<400> 1228
caatataaaa acagccttaa ctacca 26
<210> 1229
<211> 24
<212> DNA
<213> Artifical
<400> 1229
ctgagaagta aaagagcagt tctg 24
<210> 1230
<211> 28
<212> DNA
<213> Artifical
<400> 1230
ggtaaatttg ttttaaggaa aaggaagc 28
<210> 1231
<211> 24
<212> DNA
<213> Artifical
<400> 1231
ggtctctatc aaagaaactt gcct 24
<210> 1232
<211> 22
<212> DNA
<213> Artifical
<400> 1232
acataagagg gctgaatgag gg 22
<210> 1233
<211> 24
<212> DNA
<213> Artifical
<400> 1233
agaaatagaa ccattccttg cctc 24
<210> 1234
<211> 17
<212> DNA
<213> Artifical
<400> 1234
gagcccgaga gcgaatt 17
<210> 1235
<211> 21
<212> DNA
<213> Artifical
<400> 1235
aagaccctcc agaccaccat c 21
<210> 1236
<211> 17
<212> DNA
<213> Artifical
<400> 1236
agtggggtgc ctcagtg 17
<210> 1237
<211> 18
<212> DNA
<213> Artifical
<400> 1237
aaatccctac ccagcccc 18
<210> 1238
<211> 21
<212> DNA
<213> Artifical
<400> 1238
agcacttcag acaaggtcct g 21
<210> 1239
<211> 22
<212> DNA
<213> Artifical
<400> 1239
agtcacttcc ttggcacaga ag 22
<210> 1240
<211> 21
<212> DNA
<213> Artifical
<400> 1240
actcaggcta gttgcaccat t 21
<210> 1241
<211> 24
<212> DNA
<213> Artifical
<400> 1241
cccttagaga agccaaacta atgg 24
<210> 1242
<211> 24
<212> DNA
<213> Artifical
<400> 1242
tgagaaatcc ccactataac cacc 24
<210> 1243
<211> 20
<212> DNA
<213> Artifical
<400> 1243
ggctcaaatg atggcatcca 20
<210> 1244
<211> 24
<212> DNA
<213> Artifical
<400> 1244
tgagaactag tcatgccaag gaat 24
<210> 1245
<211> 24
<212> DNA
<213> Artifical
<400> 1245
agaaaagcag ccgatatttc ttgt 24
<210> 1246
<211> 24
<212> DNA
<213> Artifical
<400> 1246
agactgctgt gattgtgtgt tttt 24
<210> 1247
<211> 24
<212> DNA
<213> Artifical
<400> 1247
agttagacga ggggaataaa ggtg 24
<210> 1248
<211> 24
<212> DNA
<213> Artifical
<400> 1248
ataggtagca ccccctagaa aaag 24
<210> 1249
<211> 19
<212> DNA
<213> Artifical
<400> 1249
cagcaagaac tcctgagcg 19
<210> 1250
<211> 19
<212> DNA
<213> Artifical
<400> 1250
tggaaaggct ctggagtcg 19
<210> 1251
<211> 21
<212> DNA
<213> Artifical
<400> 1251
ttggtcgagg tcagtgaaca g 21
<210> 1252
<211> 18
<212> DNA
<213> Artifical
<400> 1252
tgcaggtggt acggtagg 18
<210> 1253
<211> 17
<212> DNA
<213> Artifical
<400> 1253
agcgagctgt actgggg 17
<210> 1254
<211> 16
<212> DNA
<213> Artifical
<400> 1254
gcaccggggt ctcgaa 16
<210> 1255
<211> 21
<212> DNA
<213> Artifical
<400> 1255
agaaagtcct gaaaacaggg c 21
<210> 1256
<211> 18
<212> DNA
<213> Artifical
<400> 1256
tggatggagc cgcagttc 18
<210> 1257
<211> 17
<212> DNA
<213> Artifical
<400> 1257
atggtaccgc tgggtcc 17
<210> 1258
<211> 25
<212> DNA
<213> Artifical
<400> 1258
cacttgagta tctttcattt gctga 25
<210> 1259
<211> 25
<212> DNA
<213> Artifical
<400> 1259
ctgaactgtt ggtttagatt attgt 25
<210> 1260
<211> 24
<212> DNA
<213> Artifical
<400> 1260
aaataaaggc acgagaatca cgtt 24
<210> 1261
<211> 17
<212> DNA
<213> Artifical
<400> 1261
ttgggaggat gcgtggc 17
<210> 1262
<211> 24
<212> DNA
<213> Artifical
<400> 1262
gttgaaattt gtcatggatg gagg 24
<210> 1263
<211> 24
<212> DNA
<213> Artifical
<400> 1263
tgtgtgtgtt tgcatgtatg tgtt 24
<210> 1264
<211> 24
<212> DNA
<213> Artifical
<400> 1264
gtaccctcag aatccattct tact 24
<210> 1265
<211> 18
<212> DNA
<213> Artifical
<400> 1265
tggttgcttg ctctgcag 18
<210> 1266
<211> 20
<212> DNA
<213> Artifical
<400> 1266
atgaggcctc ggtcatgaag 20
<210> 1267
<211> 18
<212> DNA
<213> Artifical
<400> 1267
tttgggtgag ggcttggg 18
<210> 1268
<211> 24
<212> DNA
<213> Artifical
<400> 1268
gtcccgtcaa atattgcaaa aatt 24
<210> 1269
<211> 21
<212> DNA
<213> Artifical
<400> 1269
tccttctcct cctctcttcc t 21
<210> 1270
<211> 19
<212> DNA
<213> Artifical
<400> 1270
ctgatgacat tgttggccc 19
<210> 1271
<211> 24
<212> DNA
<213> Artifical
<400> 1271
cctaacaatc ttgtgggatg agtt 24
<210> 1272
<211> 24
<212> DNA
<213> Artifical
<400> 1272
ccttttattc tccagtgtca cttt 24
<210> 1273
<211> 18
<212> DNA
<213> Artifical
<400> 1273
tcgaggacct ggtctcca 18
<210> 1274
<211> 23
<212> DNA
<213> Artifical
<400> 1274
tgtgtgtttg tggtgtgtgt atg 23
<210> 1275
<211> 24
<212> DNA
<213> Artifical
<400> 1275
ccctcatgat gtctttaact tgtg 24
<210> 1276
<211> 24
<212> DNA
<213> Artifical
<400> 1276
gcattagatt gttgggtgag taac 24
<210> 1277
<211> 24
<212> DNA
<213> Artifical
<400> 1277
cttacctttg aatcttcccc catt 24
<210> 1278
<211> 24
<212> DNA
<213> Artifical
<400> 1278
gttgaatgac tagatgtgtg tgtt 24
<210> 1279
<211> 23
<212> DNA
<213> Artifical
<400> 1279
aagttacgtc tgattcgagg aga 23
<210> 1280
<211> 24
<212> DNA
<213> Artifical
<400> 1280
atcttctcac catggagaat catg 24
<210> 1281
<211> 24
<212> DNA
<213> Artifical
<400> 1281
gaaagatgcc tgagatgtct gaag 24
<210> 1282
<211> 24
<212> DNA
<213> Artifical
<400> 1282
atatgcagac aggaattgga caaa 24
<210> 1283
<211> 24
<212> DNA
<213> Artifical
<400> 1283
atcacgactg ttctttaact acgc 24
<210> 1284
<211> 24
<212> DNA
<213> Artifical
<400> 1284
ttacagagaa agctactcat cccg 24
<210> 1285
<211> 18
<212> DNA
<213> Artifical
<400> 1285
ggcgtggaag agaaggac 18
<210> 1286
<211> 16
<212> DNA
<213> Artifical
<400> 1286
cctggggctt agagac 16
<210> 1287
<211> 19
<212> DNA
<213> Artifical
<400> 1287
atgctggagt atgagcccg 19
<210> 1288
<211> 19
<212> DNA
<213> Artifical
<400> 1288
actgacggcc actctcttg 19
<210> 1289
<211> 17
<212> DNA
<213> Artifical
<400> 1289
gtgctggagg tggaggg 17
<210> 1290
<211> 24
<212> DNA
<213> Artifical
<400> 1290
cccagaaaat tggtgctgat aatg 24
<210> 1291
<211> 24
<212> DNA
<213> Artifical
<400> 1291
agcacaatgt ggctagtaat caaa 24
<210> 1292
<211> 24
<212> DNA
<213> Artifical
<400> 1292
aagtatgcat agtgttcttg gtgg 24
<210> 1293
<211> 19
<212> DNA
<213> Artifical
<400> 1293
tcaccagtcc ccatctcct 19
<210> 1294
<211> 22
<212> DNA
<213> Artifical
<400> 1294
agcccttaca gttcttcctc tt 22
<210> 1295
<211> 18
<212> DNA
<213> Artifical
<400> 1295
cgtttcccca gggagtct 18
<210> 1296
<211> 24
<212> DNA
<213> Artifical
<400> 1296
gacagaatca ttaagggcca taca 24
<210> 1297
<211> 22
<212> DNA
<213> Artifical
<400> 1297
tcatttctcc tccatcctca gg 22
<210> 1298
<211> 20
<212> DNA
<213> Artifical
<400> 1298
gcatctttcc ctggaaggaa 20
<210> 1299
<211> 24
<212> DNA
<213> Artifical
<400> 1299
tcatgataca ggtgtcacca catc 24
<210> 1300
<211> 24
<212> DNA
<213> Artifical
<400> 1300
ttttctgcat ttatggctgg gaat 24
<210> 1301
<211> 24
<212> DNA
<213> Artifical
<400> 1301
aacagtgtct tttggttttc ctct 24
<210> 1302
<211> 24
<212> DNA
<213> Artifical
<400> 1302
ttcccatggt tatgtggtca tttg 24
<210> 1303
<211> 22
<212> DNA
<213> Artifical
<400> 1303
tgtctatgga gccatcactc tc 22
<210> 1304
<211> 20
<212> DNA
<213> Artifical
<400> 1304
agaggcattc tcgtgtgtcc 20
<210> 1305
<211> 18
<212> DNA
<213> Artifical
<400> 1305
aggactgact gcagctcc 18
<210> 1306
<211> 24
<212> DNA
<213> Artifical
<400> 1306
cacataatgg tcaatctcca actg 24
<210> 1307
<211> 17
<212> DNA
<213> Artifical
<400> 1307
ctccagtggc tcaggga 17
<210> 1308
<211> 22
<212> DNA
<213> Artifical
<400> 1308
aaacatgcct gtggatggaa ga 22
<210> 1309
<211> 21
<212> DNA
<213> Artifical
<400> 1309
atgtccgtgt ccaccatgaa c 21
<210> 1310
<211> 20
<212> DNA
<213> Artifical
<400> 1310
ttcatgcttg ccattccctc 20
<210> 1311
<211> 19
<212> DNA
<213> Artifical
<400> 1311
acccagaccc acagacatg 19
<210> 1312
<211> 22
<212> DNA
<213> Artifical
<400> 1312
ctgcttggac tggaatgaag ag 22
<210> 1313
<211> 24
<212> DNA
<213> Artifical
<400> 1313
cataacagca ggtcaaagag tgag 24
<210> 1314
<211> 19
<212> DNA
<213> Artifical
<400> 1314
tccaggatgc cattgcctg 19
<210> 1315
<211> 20
<212> DNA
<213> Artifical
<400> 1315
tgacagtggc tcccgtgtag 20
<210> 1316
<211> 22
<212> DNA
<213> Artifical
<400> 1316
tccactccct actatctctg gg 22
<210> 1317
<211> 22
<212> DNA
<213> Artifical
<400> 1317
aggggatgcc ttgtgctaat tc 22
<210> 1318
<211> 24
<212> DNA
<213> Artifical
<400> 1318
acattggcat cctctctcta gaag 24
<210> 1319
<211> 19
<212> DNA
<213> Artifical
<400> 1319
ggatgcaaca ggagagtgg 19
<210> 1320
<211> 22
<212> DNA
<213> Artifical
<400> 1320
ggcttttgtg aacccaaaga ac 22
<210> 1321
<211> 20
<212> DNA
<213> Artifical
<400> 1321
ctagactcct gggtctgagg 20
<210> 1322
<211> 21
<212> DNA
<213> Artifical
<400> 1322
actgatgatc acgtagggga c 21
<210> 1323
<211> 19
<212> DNA
<213> Artifical
<400> 1323
ctgctgctcc aggtagtac 19
<210> 1324
<211> 17
<212> DNA
<213> Artifical
<400> 1324
tggtggctga gcactgt 17
<210> 1325
<211> 17
<212> DNA
<213> Artifical
<400> 1325
tggcactgcg tccagag 17
<210> 1326
<211> 20
<212> DNA
<213> Artifical
<400> 1326
cagagaccac aaagcaccat 20
<210> 1327
<211> 17
<212> DNA
<213> Artifical
<400> 1327
agtgccgcac cacttcc 17
<210> 1328
<211> 19
<212> DNA
<213> Artifical
<400> 1328
aggcgtgagg ggtcttatc 19
<210> 1329
<211> 17
<212> DNA
<213> Artifical
<400> 1329
cactggctcc aaaccca 17
<210> 1330
<211> 17
<212> DNA
<213> Artifical
<400> 1330
ggggaggggg cttcaaa 17
<210> 1331
<211> 20
<212> DNA
<213> Artifical
<400> 1331
ttctcatgat cccctcccag 20
<210> 1332
<211> 17
<212> DNA
<213> Artifical
<400> 1332
ggcatgctca ccagtgg 17
<210> 1333
<211> 18
<212> DNA
<213> Artifical
<400> 1333
aggttggtcc tctgcacc 18
<210> 1334
<211> 24
<212> DNA
<213> Artifical
<400> 1334
gtaggcatgt ggttctatac aaat 24
<210> 1335
<211> 24
<212> DNA
<213> Artifical
<400> 1335
tcagtgctat gagtgaggac ttat 24
<210> 1336
<211> 24
<212> DNA
<213> Artifical
<400> 1336
ttaaatatgt atacctgccg ggcc 24
<210> 1337
<211> 34
<212> DNA
<213> Artifical
<400> 1337
ataattaaat aatgacatta caacttattg tcaa 34
<210> 1338
<211> 24
<212> DNA
<213> Artifical
<400> 1338
tagcatctgt aacgttccca aaaa 24
<210> 1339
<211> 24
<212> DNA
<213> Artifical
<400> 1339
agtcaagttt ctgttgcatg aact 24
<210> 1340
<211> 30
<212> DNA
<213> Artifical
<400> 1340
agaacttgat acaaatttgc taataaaaag 30
<210> 1341
<211> 23
<212> DNA
<213> Artifical
<400> 1341
ttcgctggca aaaataaaga tgc 23
<210> 1342
<211> 24
<212> DNA
<213> Artifical
<400> 1342
tttctgctac actcaaaggc aaat 24
<210> 1343
<211> 23
<212> DNA
<213> Artifical
<400> 1343
actttgtatc ccacggactc tac 23
<210> 1344
<211> 24
<212> DNA
<213> Artifical
<400> 1344
atctggtaaa catagcagct gaag 24
<210> 1345
<211> 24
<212> DNA
<213> Artifical
<400> 1345
ttatgccttt accatgcact atcc 24
<210> 1346
<211> 22
<212> DNA
<213> Artifical
<400> 1346
aactcaatcc tcggactttt ct 22
<210> 1347
<211> 19
<212> DNA
<213> Artifical
<400> 1347
aggcagacaa gaaggagga 19
<210> 1348
<211> 20
<212> DNA
<213> Artifical
<400> 1348
aagaaacaca cggggaggtg 20
<210> 1349
<211> 24
<212> DNA
<213> Artifical
<400> 1349
caacacctgc acattctcaa attc 24
<210> 1350
<211> 18
<212> DNA
<213> Artifical
<400> 1350
tgccccctct cctctgag 18
<210> 1351
<211> 24
<212> DNA
<213> Artifical
<400> 1351
tgctcttctt ggattcacaa agtc 24
<210> 1352
<211> 19
<212> DNA
<213> Artifical
<400> 1352
tcattctgga cggctgtga 19
<210> 1353
<211> 19
<212> DNA
<213> Artifical
<400> 1353
cttctgtaat atctgaggc 19
<210> 1354
<211> 24
<212> DNA
<213> Artifical
<400> 1354
aaataaaatc aagccagtcc acgg 24
<210> 1355
<211> 21
<212> DNA
<213> Artifical
<400> 1355
actcctgact agtctccctc a 21
<210> 1356
<211> 24
<212> DNA
<213> Artifical
<400> 1356
aaatcttgac ttgcctcatc tgtt 24
<210> 1357
<211> 22
<212> DNA
<213> Artifical
<400> 1357
tgcaacaggt tgtcaatctt gg 22
<210> 1358
<211> 24
<212> DNA
<213> Artifical
<400> 1358
accttctaga ctccactttc tcct 24
<210> 1359
<211> 19
<212> DNA
<213> Artifical
<400> 1359
gaccacgtga tcaccaggt 19
<210> 1360
<211> 24
<212> DNA
<213> Artifical
<400> 1360
aaacacaacc tggaagacaa acta 24
<210> 1361
<211> 24
<212> DNA
<213> Artifical
<400> 1361
cttcctggca tttcatagat acct 24
<210> 1362
<211> 24
<212> DNA
<213> Artifical
<400> 1362
aaaggggttc tgctgaaatg aaat 24
<210> 1363
<211> 24
<212> DNA
<213> Artifical
<400> 1363
caatctgatt gttctccgga agaa 24
<210> 1364
<211> 17
<212> DNA
<213> Artifical
<400> 1364
gtcaagatcg ccctcgc 17
<210> 1365
<211> 24
<212> DNA
<213> Artifical
<400> 1365
ttacaaaacc aggaatgctt ttgg 24
<210> 1366
<211> 24
<212> DNA
<213> Artifical
<400> 1366
gacaatgcag actaacacta agga 24
<210> 1367
<211> 24
<212> DNA
<213> Artifical
<400> 1367
atttcttagg tgtcagattt tccc 24
<210> 1368
<211> 17
<212> DNA
<213> Artifical
<400> 1368
agcctccaca actccgg 17
<210> 1369
<211> 25
<212> DNA
<213> Artifical
<400> 1369
gattcgtgtt ttaaaagcat caagt 25
<210> 1370
<211> 25
<212> DNA
<213> Artifical
<400> 1370
ctgtgaattc aagaaaactt aagga 25
<210> 1371
<211> 24
<212> DNA
<213> Artifical
<400> 1371
ccagaaatgg tgtaagtaca gagt 24
<210> 1372
<211> 24
<212> DNA
<213> Artifical
<400> 1372
gctggctcta ttttgataga ggaa 24
<210> 1373
<211> 24
<212> DNA
<213> Artifical
<400> 1373
gcaaggaaaa ccattcttcc taac 24
<210> 1374
<211> 24
<212> DNA
<213> Artifical
<400> 1374
aaccacataa gaaactctca tggc 24
<210> 1375
<211> 30
<212> DNA
<213> Artifical
<400> 1375
tcaaaatatg acagacatca attatttact 30
<210> 1376
<211> 24
<212> DNA
<213> Artifical
<400> 1376
cactgaacag ttgtctatgt ccat 24
<210> 1377
<211> 26
<212> DNA
<213> Artifical
<400> 1377
tccaaaattt ctacaatgaa cacatt 26
<210> 1378
<211> 26
<212> DNA
<213> Artifical
<400> 1378
agaagtctta agtttcagta caaaca 26
<210> 1379
<211> 24
<212> DNA
<213> Artifical
<400> 1379
atgtcaaact gtattggggt gtct 24
<210> 1380
<211> 25
<212> DNA
<213> Artifical
<400> 1380
cttagtagca gagcaaatat aaaca 25
<210> 1381
<211> 24
<212> DNA
<213> Artifical
<400> 1381
agcttaaatc atgtggaact cacc 24
<210> 1382
<211> 26
<212> DNA
<213> Artifical
<400> 1382
gtaagtggtt atgacagaga tattct 26
<210> 1383
<211> 24
<212> DNA
<213> Artifical
<400> 1383
atcttggatt cttaccagtt cagc 24
<210> 1384
<211> 24
<212> DNA
<213> Artifical
<400> 1384
cagcccttca tttggaaatt caaa 24
<210> 1385
<211> 24
<212> DNA
<213> Artifical
<400> 1385
caacatagtt gttaccttct gcca 24
<210> 1386
<211> 20
<212> DNA
<213> Artifical
<400> 1386
agctgcacaa tgctgaactg 20
<210> 1387
<211> 19
<212> DNA
<213> Artifical
<400> 1387
aagctgctca accctccag 19
<210> 1388
<211> 18
<212> DNA
<213> Artifical
<400> 1388
tgcccagggt gaatcctc 18
<210> 1389
<211> 19
<212> DNA
<213> Artifical
<400> 1389
agatagggca ggccaggat 19
<210> 1390
<211> 24
<212> DNA
<213> Artifical
<400> 1390
cggctgtaag acactctttg ttag 24
<210> 1391
<211> 19
<212> DNA
<213> Artifical
<400> 1391
gaagttctcc caggccttg 19
<210> 1392
<211> 22
<212> DNA
<213> Artifical
<400> 1392
ggaagaagag atagagcagg ta 22
<210> 1393
<211> 24
<212> DNA
<213> Artifical
<400> 1393
gtacacatag aaccagcaga acag 24
<210> 1394
<211> 18
<212> DNA
<213> Artifical
<400> 1394
ttggtgacgt cgtcctcc 18
<210> 1395
<211> 24
<212> DNA
<213> Artifical
<400> 1395
gaatgggaag aaaaagaagt cgaa 24
<210> 1396
<211> 19
<212> DNA
<213> Artifical
<400> 1396
tcccacagac aaagcctgt 19
<210> 1397
<211> 24
<212> DNA
<213> Artifical
<400> 1397
gactcacata taaacaagcg tcag 24
<210> 1398
<211> 24
<212> DNA
<213> Artifical
<400> 1398
aatcaaaggc ctatgtgatt ggag 24
<210> 1399
<211> 24
<212> DNA
<213> Artifical
<400> 1399
aggccaacaa ggaggaaata aaat 24
<210> 1400
<211> 24
<212> DNA
<213> Artifical
<400> 1400
cacagtttat tgtttgctca aagc 24
<210> 1401
<211> 24
<212> DNA
<213> Artifical
<400> 1401
gtcaaatgga accaaactca gatg 24
<210> 1402
<211> 20
<212> DNA
<213> Artifical
<400> 1402
catctgaaat gttgccagca 20
<210> 1403
<211> 22
<212> DNA
<213> Artifical
<400> 1403
attacctgtg agcctagcga ac 22
<210> 1404
<211> 24
<212> DNA
<213> Artifical
<400> 1404
cgaagccatt ctgaaactaa acat 24
<210> 1405
<211> 24
<212> DNA
<213> Artifical
<400> 1405
ttctttgctg ctccaacttt tcta 24
<210> 1406
<211> 22
<212> DNA
<213> Artifical
<400> 1406
gtttcattat agccatttaa cc 22
<210> 1407
<211> 24
<212> DNA
<213> Artifical
<400> 1407
gtcaaagctg caaacaacaa aatc 24
<210> 1408
<211> 24
<212> DNA
<213> Artifical
<400> 1408
aattttgtgt gtgtgcatta cctg 24
<210> 1409
<211> 24
<212> DNA
<213> Artifical
<400> 1409
ggaggaaatt tgaggtggta ttgt 24
<210> 1410
<211> 24
<212> DNA
<213> Artifical
<400> 1410
tgctgagaat ctgaaaagca tagg 24
<210> 1411
<211> 24
<212> DNA
<213> Artifical
<400> 1411
tcgttcattc cattcagttg agat 24
<210> 1412
<211> 24
<212> DNA
<213> Artifical
<400> 1412
acacgtactc caagacaaac taca 24
<210> 1413
<211> 24
<212> DNA
<213> Artifical
<400> 1413
ctgtagcctt acctcgagat atct 24
<210> 1414
<211> 24
<212> DNA
<213> Artifical
<400> 1414
atcaccatgc attttagtag acgc 24
<210> 1415
<211> 25
<212> DNA
<213> Artifical
<400> 1415
catctgcttc aaatgttttc taagt 25
<210> 1416
<211> 23
<212> DNA
<213> Artifical
<400> 1416
atcaacttga aactcagtga ctg 23
<210> 1417
<211> 24
<212> DNA
<213> Artifical
<400> 1417
cacagaaagc gagatcatct ttaa 24
<210> 1418
<211> 29
<212> DNA
<213> Artifical
<400> 1418
ccatctttag taactactta ctcttcagt 29
<210> 1419
<211> 24
<212> DNA
<213> Artifical
<400> 1419
tggagataca tacactacag agca 24
<210> 1420
<211> 24
<212> DNA
<213> Artifical
<400> 1420
gttctttcag agagctgtac aagt 24
<210> 1421
<211> 24
<212> DNA
<213> Artifical
<400> 1421
aaaaagaagg gataagcact ccag 24
<210> 1422
<211> 24
<212> DNA
<213> Artifical
<400> 1422
atcctatctc tccagacacc agaa 24
<210> 1423
<211> 18
<212> DNA
<213> Artifical
<400> 1423
gtccatctgg gttaccgg 18
<210> 1424
<211> 24
<212> DNA
<213> Artifical
<400> 1424
gagttcttac agtgaatttt gtgt 24
<210> 1425
<211> 21
<212> DNA
<213> Artifical
<400> 1425
agcctgttgt ccccatcaga t 21
<210> 1426
<211> 24
<212> DNA
<213> Artifical
<400> 1426
ctctgaatct tgagactgga tgtt 24
<210> 1427
<211> 24
<212> DNA
<213> Artifical
<400> 1427
agcagataca tttgtcacag gaag 24
<210> 1428
<211> 24
<212> DNA
<213> Artifical
<400> 1428
attgggtgcc cttcttagaa tacg 24
<210> 1429
<211> 17
<212> DNA
<213> Artifical
<400> 1429
gcttcagtgt accctgc 17
<210> 1430
<211> 24
<212> DNA
<213> Artifical
<400> 1430
ttttcatgct atctgcctga catg 24
<210> 1431
<211> 24
<212> DNA
<213> Artifical
<400> 1431
gcttcatcaa gagaatacct ggtt 24
<210> 1432
<211> 24
<212> DNA
<213> Artifical
<400> 1432
ttgagctgta tctgaagtcc ttcg 24
<210> 1433
<211> 24
<212> DNA
<213> Artifical
<400> 1433
agtaccatga aattcccagc aaat 24
<210> 1434
<211> 24
<212> DNA
<213> Artifical
<400> 1434
tgctggatct agttgcagag taaa 24
<210> 1435
<211> 26
<212> DNA
<213> Artifical
<400> 1435
gggacaaaag tagaaaatag tcttct 26
<210> 1436
<211> 24
<212> DNA
<213> Artifical
<400> 1436
tgttgtctac ttagctccct tcaa 24
<210> 1437
<211> 24
<212> DNA
<213> Artifical
<400> 1437
tcagtgtaga taccagattc agtg 24
<210> 1438
<211> 24
<212> DNA
<213> Artifical
<400> 1438
gttctaccac agtatccaac catt 24
<210> 1439
<211> 24
<212> DNA
<213> Artifical
<400> 1439
ggaactgtga ctgaggaaca aatt 24
<210> 1440
<211> 24
<212> DNA
<213> Artifical
<400> 1440
attattaaac tgggtctcca tggc 24
<210> 1441
<211> 24
<212> DNA
<213> Artifical
<400> 1441
tttactcgca tctggaaaga gttt 24
<210> 1442
<211> 24
<212> DNA
<213> Artifical
<400> 1442
cctggactgg aagttatgag tttt 24
<210> 1443
<211> 19
<212> DNA
<213> Artifical
<400> 1443
agtccaagcc atgcatcga 19
<210> 1444
<211> 20
<212> DNA
<213> Artifical
<400> 1444
aggtggaacg caatgtctga 20
<210> 1445
<211> 26
<212> DNA
<213> Artifical
<400> 1445
cttcttaaaa tgttttcaag aactgc 26
<210> 1446
<211> 24
<212> DNA
<213> Artifical
<400> 1446
aaactggaag ctgtaaggga aaat 24
<210> 1447
<211> 24
<212> DNA
<213> Artifical
<400> 1447
gtcagccact gcatattcat tttt 24
<210> 1448
<211> 22
<212> DNA
<213> Artifical
<400> 1448
acatgtacat acacaccggg aa 22
<210> 1449
<211> 19
<212> DNA
<213> Artifical
<400> 1449
cacgtcaagc caagcatca 19
<210> 1450
<211> 21
<212> DNA
<213> Artifical
<400> 1450
atcgacccgg ttcgattaga a 21
<210> 1451
<211> 24
<212> DNA
<213> Artifical
<400> 1451
acttcaaggc acacaaatca gttc 24
<210> 1452
<211> 18
<212> DNA
<213> Artifical
<400> 1452
atgaggtggg aggaggga 18
<210> 1453
<211> 17
<212> DNA
<213> Artifical
<400> 1453
accctcgacc accgact 17
<210> 1454
<211> 23
<212> DNA
<213> Artifical
<400> 1454
tgatgtaatg gacctgccct atg 23
<210> 1455
<211> 23
<212> DNA
<213> Artifical
<400> 1455
tacatggagg agatgcagaa tgt 23
<210> 1456
<211> 24
<212> DNA
<213> Artifical
<400> 1456
atgatctcct gctactacga agac 24
<210> 1457
<211> 24
<212> DNA
<213> Artifical
<400> 1457
tcccaaaacc tctcaaagct atgg 24
<210> 1458
<211> 24
<212> DNA
<213> Artifical
<400> 1458
tcttccagct cttcgactac atct 24
<210> 1459
<211> 21
<212> DNA
<213> Artifical
<400> 1459
aggctgacac acttctctct g 21
<210> 1460
<211> 17
<212> DNA
<213> Artifical
<400> 1460
tccccctccc tgtgcag 17
<210> 1461
<211> 23
<212> DNA
<213> Artifical
<400> 1461
aggtcagatg gaacctcttc ttc 23
<210> 1462
<211> 20
<212> DNA
<213> Artifical
<400> 1462
cttacgctcc aaggctacag 20
<210> 1463
<211> 20
<212> DNA
<213> Artifical
<400> 1463
gagcaataac cccaagctct 20
<210> 1464
<211> 24
<212> DNA
<213> Artifical
<400> 1464
aagctaaggt tcttctcttg cttt 24
<210> 1465
<211> 23
<212> DNA
<213> Artifical
<400> 1465
gaatcatctc tgcacaggta ctc 23
<210> 1466
<211> 24
<212> DNA
<213> Artifical
<400> 1466
ttctgagttc aggctaggaa catg 24
<210> 1467
<211> 24
<212> DNA
<213> Artifical
<400> 1467
ttctgagttc aggctaggaa catg 24
<210> 1468
<211> 20
<212> DNA
<213> Artifical
<400> 1468
agtgttgcct aaggggagac 20
<210> 1469
<211> 24
<212> DNA
<213> Artifical
<400> 1469
agcatcattc atgttctttg ggat 24
<210> 1470
<211> 24
<212> DNA
<213> Artifical
<400> 1470
gaaggagaaa gcatagagag agag 24
<210> 1471
<211> 24
<212> DNA
<213> Artifical
<400> 1471
ttggctttgt cttatagcct cctc 24
<210> 1472
<211> 23
<212> DNA
<213> Artifical
<400> 1472
cttcccttag atcaggagag ttg 23
<210> 1473
<211> 19
<212> DNA
<213> Artifical
<400> 1473
tgggcaatca ctcaccagg 19
<210> 1474
<211> 24
<212> DNA
<213> Artifical
<400> 1474
caaccttttt ccaactccac aaag 24
<210> 1475
<211> 23
<212> DNA
<213> Artifical
<400> 1475
acatagcctt ctcttcctgt gac 23
<210> 1476
<211> 19
<212> DNA
<213> Artifical
<400> 1476
cttcccctct cactccagg 19
<210> 1477
<211> 24
<212> DNA
<213> Artifical
<400> 1477
cctgctcact ctaagactga aaat 24
<210> 1478
<211> 17
<212> DNA
<213> Artifical
<400> 1478
tggactcagg atgcggc 17
<210> 1479
<211> 19
<212> DNA
<213> Artifical
<400> 1479
cacctctggc catgtactc 19
<210> 1480
<211> 18
<212> DNA
<213> Artifical
<400> 1480
tacttcacca acctgcgc 18
<210> 1481
<211> 24
<212> DNA
<213> Artifical
<400> 1481
aagataaggc cctaggtttc cctt 24
<210> 1482
<211> 24
<212> DNA
<213> Artifical
<400> 1482
taagtgacaa cttccatcag gagg 24
<210> 1483
<211> 18
<212> DNA
<213> Artifical
<400> 1483
caggttgcgg atgacctc 18
<210> 1484
<211> 22
<212> DNA
<213> Artifical
<400> 1484
tggattccgg gtctgagaaa ag 22
<210> 1485
<211> 18
<212> DNA
<213> Artifical
<400> 1485
tgtgggcacg tagactgg 18
<210> 1486
<211> 18
<212> DNA
<213> Artifical
<400> 1486
ccatgtaagc cgggtagg 18
<210> 1487
<211> 17
<212> DNA
<213> Artifical
<400> 1487
gaggcacgtg cttacca 17
<210> 1488
<211> 20
<212> DNA
<213> Artifical
<400> 1488
cgtggagctt catgtagagg 20
<210> 1489
<211> 24
<212> DNA
<213> Artifical
<400> 1489
tgcctaaccc ggaagatatg attc 24
<210> 1490
<211> 20
<212> DNA
<213> Artifical
<400> 1490
caagtcccac ccagtactag 20
<210> 1491
<211> 20
<212> DNA
<213> Artifical
<400> 1491
tatcctccaa gtcccaggtc 20
<210> 1492
<211> 19
<212> DNA
<213> Artifical
<400> 1492
tgtgctgggc tctttctca 19
<210> 1493
<211> 24
<212> DNA
<213> Artifical
<400> 1493
aggcatctct tcttgaagtt cttg 24
<210> 1494
<211> 19
<212> DNA
<213> Artifical
<400> 1494
cagtgctgag gaacccaag 19
<210> 1495
<211> 20
<212> DNA
<213> Artifical
<400> 1495
agcttcctct caacctgtgg 20
<210> 1496
<211> 24
<212> DNA
<213> Artifical
<400> 1496
gataaagaac ggaaatgtgg acag 24
<210> 1497
<211> 21
<212> DNA
<213> Artifical
<400> 1497
ctagttggtg acaggacaag a 21
<210> 1498
<211> 24
<212> DNA
<213> Artifical
<400> 1498
aactcactga ccagcttgta gaag 24
<210> 1499
<211> 23
<212> DNA
<213> Artifical
<400> 1499
taaaaagagg ccctcaagtc ttg 23
<210> 1500
<211> 23
<212> DNA
<213> Artifical
<400> 1500
tcttaccctg ttttggcaaa gtt 23
<210> 1501
<211> 17
<212> DNA
<213> Artifical
<400> 1501
aggggtggcc tcctggt 17
<210> 1502
<211> 24
<212> DNA
<213> Artifical
<400> 1502
ataagatgag ctgggcatga aaaa 24
Claims (2)
1.一种扩增单基因糖尿病致病基因的引物组合,其特征在于,所述引物组合由751对引物组成;
所述751对引物,为序列SEQ ID No.1-751的正向引物,依次与序列为SEQ ID No.752-1502的反向引物配对,形成的组合;
所述单基因糖尿病致病基因为LMNA、PPP1R15B、KLF11、DCAF17、NEUROD1、PPARG、APPL1、WFS1、TRMT10A、CISD2、PIK3R1、ZFP57、RFX6、ZBTB2、GCK、PAX4、BLK、GATA4、CEL、PCBD1、SLC29A3、INS、ABCC8、KCNJ11、PAX6、HNF1A、PDX1、DNAJC3、PLIN1、HNF1B、GATA6、INSR、DYRK1B、AKT2、POLD1、HNF4A和MTTL1。
2.一种试剂盒,其包含权利要求1所述引物组合。
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Citations (3)
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CN101619351A (zh) * | 2009-01-23 | 2010-01-06 | 中南大学 | 一种糖尿病药物治疗相关基因突变的检测方法及其专用芯片和试剂盒 |
WO2014135653A1 (en) * | 2013-03-06 | 2014-09-12 | Royal College Of Surgeons In Ireland | Diagnosis and treatment of metabolic disorders |
CN106554999A (zh) * | 2016-10-25 | 2017-04-05 | 大连晶泰生物技术有限公司 | 高通量检测新生儿糖尿病致病基因突变位点的测序文库构建方法、试剂盒及其用途 |
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CN101619351A (zh) * | 2009-01-23 | 2010-01-06 | 中南大学 | 一种糖尿病药物治疗相关基因突变的检测方法及其专用芯片和试剂盒 |
WO2014135653A1 (en) * | 2013-03-06 | 2014-09-12 | Royal College Of Surgeons In Ireland | Diagnosis and treatment of metabolic disorders |
CN106554999A (zh) * | 2016-10-25 | 2017-04-05 | 大连晶泰生物技术有限公司 | 高通量检测新生儿糖尿病致病基因突变位点的测序文库构建方法、试剂盒及其用途 |
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