CN111686287B - Preparation method and application of microbial bacteriostatic deodorant - Google Patents
Preparation method and application of microbial bacteriostatic deodorant Download PDFInfo
- Publication number
- CN111686287B CN111686287B CN202010617315.6A CN202010617315A CN111686287B CN 111686287 B CN111686287 B CN 111686287B CN 202010617315 A CN202010617315 A CN 202010617315A CN 111686287 B CN111686287 B CN 111686287B
- Authority
- CN
- China
- Prior art keywords
- microbial
- temperature
- preparing
- steps
- removing agent
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L9/00—Disinfection, sterilisation or deodorisation of air
- A61L9/01—Deodorant compositions
- A61L9/014—Deodorant compositions containing sorbent material, e.g. activated carbon
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/20—Bacteria; Substances produced thereby or obtained therefrom
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/20—Bacteria; Substances produced thereby or obtained therefrom
- A01N63/22—Bacillus
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/06—Coniferophyta [gymnosperms], e.g. cypress
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/20—Fabaceae or Leguminosae [Pea or Legume family], e.g. pea, lentil, soybean, clover, acacia, honey locust, derris or millettia
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/36—Rutaceae [Rue family], e.g. lime, orange, lemon, corktree or pricklyash
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/38—Solanaceae [Potato family], e.g. nightshade, tomato, tobacco or chilli pepper
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L9/00—Disinfection, sterilisation or deodorisation of air
- A61L9/01—Deodorant compositions
- A61L9/013—Deodorant compositions containing animal or plant extracts, or vegetable material
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2209/00—Aspects relating to disinfection, sterilisation or deodorisation of air
- A61L2209/20—Method-related aspects
- A61L2209/21—Use of chemical compounds for treating air or the like
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2209/00—Aspects relating to disinfection, sterilisation or deodorisation of air
- A61L2209/20—Method-related aspects
- A61L2209/22—Treatment by sorption, e.g. absorption, adsorption, chemisorption, scrubbing, wet cleaning
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Dentistry (AREA)
- Biotechnology (AREA)
- Environmental Sciences (AREA)
- Wood Science & Technology (AREA)
- Plant Pathology (AREA)
- Microbiology (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Agronomy & Crop Science (AREA)
- Mycology (AREA)
- Botany (AREA)
- Animal Behavior & Ethology (AREA)
- Veterinary Medicine (AREA)
- Public Health (AREA)
- Epidemiology (AREA)
- Pest Control & Pesticides (AREA)
- Virology (AREA)
- Materials Engineering (AREA)
- Chemical & Material Sciences (AREA)
- Disinfection, Sterilisation Or Deodorisation Of Air (AREA)
Abstract
The invention discloses a preparation method and application of a microbial bacteriostatic deodorant, and relates to the technical field of preparation of daily cleaning products, wherein the preparation method comprises the following steps: (1) preparing a microbial fermentation broth; (2) fermenting by microorganisms; (3) adding plant additives into the fermentation product, uniformly mixing, and standing; (4) granulating; (5) drying by a program; (6) coating a porous surface layer on the surface of the dried particles; (7) and (4) rapidly cooling after heat treatment to obtain the microbial bacteriostatic deodorant. The microbial bacteriostatic deodorant provided by the invention breaks through the form of the traditional bacteriostatic deodorant, can be well formed while ensuring the microbial activity, and the novel microbial deodorant in a solid form can be suitable for various complex environments, is not easy to deteriorate and has a long service life.
Description
Technical Field
The invention relates to the technical field of preparation of daily cleaning products, in particular to a preparation method and application of a microbial bacteriostatic odor removing agent.
Background
The odor-removing agent can remove or counteract off-flavor, and can be physical odor-removing agent, chemical odor-removing agent, biological odor-removing agent, microbial odor-removing agent, etc. Along with the improvement of the living standard of people in China, people pay more and more attention to the pollution of peculiar smell (mainly referring to the stink and the smell which can cause people to be uncomfortable). Therefore, the development and use of odor eliminators has been a focus of research. The peculiar smell substances are various, and different groups exist in molecular structures of different types of substances, so that different smells and threshold values exist. There are tens of thousands of substances that produce off-flavors, and off-flavor gases can be classified into 5 types according to their composition: sulfur-containing compounds such as hydrogen sulfide, sulfur dioxide, mercaptans, and the like; ② nitrogen-containing compounds such as ammonia, amines, indole, etc.; ③ halogens and derivatives, such as chlorine, halogenated hydrocarbons, etc.; hydrocarbons and aromatic hydrocarbons; oxygen-containing organic substances, such as alcohol, atmosphere, aldehyde, ketone, etc. Among the odorous substances, thiols, ammonia and sulfuration are more harmful to human healthHydrogen, dimethyl sulfide, trimethylamine, formaldehyde, styrene, chromic acid, phenols, and the like. Odor pollution mainly harms the following circulatory systems of the human body: respiratory system, circulatory system, digestive system, endocrine system and nervous system seriously affect the mental state of people and reduce the life quality of people. The most common hydrogen sulfide gas has peculiar smell and strong toxicity, and the perception quantity of human body is 0.00037mg/m3Gases containing 0.1% hydrogen sulfide can poison the human body, and hydrogen sulfide with higher concentration can kill the human body in a short time.
At present, the odor removing agent used for living environments such as farms, toilet sides and the like is mainly a chemical odor removing agent, and on the basis of no environmental protection and high cost, the problems of difficult recovery or treatment of toxic substances and resources and the like can also exist; the physical smell-removing agent has little effect under the environment with higher concentration of the peculiar smell gas; the pure plant odor removing agent has large variety and dosage of required plants, complex preparation method and overhigh cost, and the dosage of the odor removing agent in the environment is usually very large, so the practical application of the odor removing agent in the environment is limited; at present, no microbial odor-removing agent for such environment is reported.
Disclosure of Invention
Aiming at the problems of the existing odor removing agent, the invention provides a preparation method and application of a microbial bacteriostatic odor removing agent which can be applied to living environment with high concentration of odor gas, and the technical scheme is as follows:
the invention provides a preparation method of a microbial bacteriostatic odor removing agent, which comprises the following steps:
(1) preparing a microbial fermentation broth;
(2) fermenting by microorganisms;
(3) adding plant additives into the fermentation product, uniformly mixing, and standing;
(4) granulating;
(5) drying by a program;
(6) coating a porous surface layer on the surface of the dried particles;
(7) and (4) rapidly cooling after heat treatment to obtain the microbial bacteriostatic deodorant.
Preferably, the microbial fermentation broth in step (1) comprises: plant waste, complex microbial liquid; the plant waste is one or more than two of straw, wheat husk, rice husk and corncob; the compound microorganism liquid comprises enterobacter hopcalis, bacillus subtilis, bacillus sonoralis, leuconostoc mesenteroides, bacillus amylovorus, weissella schinensis, bacillus Oleronius, pediococcus pentosaceus, lactobacillus rhamnosus and microbacterium thermophilus; the preparation method of the microbial fermentation liquid comprises the following steps: crushing the plant waste, sieving with a sieve of 150-plus-200 meshes, sterilizing at 121 ℃ for 30min under high temperature and high pressure, adding a compound microbial solution prepared by sterile water, and uniformly mixing to obtain the compound microbial solution; in the compound microorganism bacterial liquid, the bacterial concentration of the enterobacter hopcalis is 8.18-9.85 multiplied by 109The cell density of the bacillus subtilis is 5.26-6.19 multiplied by 1010The cell concentration of the Bacillus sonoralis in the strain/mL is 4.18-6.99 multiplied by 108The cell concentration of Leuconostoc mesenteroides is 2.69-4.66 × 1010The cell concentration of Bacillus amylovorus per mL is 3.78-5.12 × 109The cell density of Weissella Greek is 6.19-8.24 × 108The cell concentration of the Bacillus Oleronius per mL is 2.69-5.49 × 109The cell density of Pediococcus pentosaceus is 3.19-4.98 × 1010The cell concentration of rhamnosus bacterium is 4.88-6.13 × 1010The cell concentration of the thermophilic microbacterium is 1.59-3.08X 108Per mL; the mass ratio of the plant waste to the composite microbial liquid is (1-30) to (5-50).
Preferably, the microorganism in the step (2) is fermented, the fermentation temperature is 25-27 ℃, and the fermentation time is 72-120 h; intermittently aerating for 1-2h each time during fermentation, and 3-5h at intervals.
Preferably, the plant additive in step (3) comprises tobacco leaves, tea leaves, Chinese honeylocust fruits, orange peels and turpentine; wherein the mass ratio of the tobacco leaves, the tea leaves, the Chinese honeylocust fruits, the orange peels and the turpentine is (1-3) to (5-10) to (2-5) to (5-8) to (1-2); the mass ratio of the plant additive to the fermentation product is (1-5) to (10-100); the standing temperature is 20-25 ℃, and the standing time is 1-2 h.
Preferably, the granulation in the step (4) is carried out, the particle size is 5-15mm, and the temperature of the granulator is 20-30 ℃.
Preferably, the procedure drying in step (5) is specifically: and (3) preserving heat for a period of time after the first temperature rise, cooling, preserving heat after the second temperature rise, and naturally cooling to room temperature.
Preferably, in the above-mentioned procedural drying step: the first temperature rise is carried out, the temperature rise rate is 2-5 ℃/min, the temperature is raised to 25-30 ℃, and the heat preservation time is 20-50min after the first temperature rise; cooling at a cooling rate of 2-5 ℃/min to 20-23 ℃ for 5-10 min; and finally, heating for the second time at the heating rate of 2-5 ℃/min to 35-37 ℃, and keeping the temperature for 2-5min after the second heating.
More preferably, in the above-mentioned procedural drying step: the first temperature rise is carried out, the temperature rise rate is 2-5 ℃/min, the temperature is raised to 25-30 ℃, and the heat preservation time is 20-50min after the first temperature rise; cooling at a rate of 2-5 ℃/min to 20-23 ℃, and keeping the temperature for a period of time; finally, heating for the second time, wherein the heating rate is 2-5 ℃/min, the heating is carried out to 35-37 ℃, and the heat preservation time is 2-5min after the second heating; in temperature programming/cooling, the relationship between the holding time is as follows:
wherein, tjFor the holding time after cooling, ts1M is the holding time after the first temperature risejM is the mass of the complex microbial liquidfMass m of the plant wastezIs the quality of the plant additive.
Preferably, the wrapping porous surface layer in the step (6) is specifically: 1) preparing a surface layer wrapping liquid: preparing bamboo charcoal powder and quicklime powder into suspension with water; 2) wrapping the suspension on the surface of the dried particles obtained in the step (5); 3) drying to form a porous surface layer. Step 1), the mass ratio of the bamboo charcoal powder, the quicklime powder and the water is (8-15) to (1-3) to (10-25); the wrapping method in the step 2) comprises the following steps: rolling the dried particles obtained in the step (5), and spraying the suspension prepared in the step 1) on the surfaces of the particles; step 2) the mass ratio of the suspension wrapped on the surfaces of the dried particles to the dried particles is (2-10) to (3-7); and 3) drying, wherein the drying temperature is 30-35 ℃, and the drying time is 2-10 min.
The heat treatment in the step (7) is carried out, wherein the heat treatment temperature is 120-150 ℃, and the heat treatment time is 30-60 s; the temperature is rapidly reduced at a rate of 150-.
The drying and heat treatment operations of the present invention are carried out under hot air conditions.
Secondly, the invention also provides application of the microbial antibacterial deodorant prepared by the method in removing environment, which specifically comprises the following steps: and placing the microbial bacteriostatic odor removing agent in the environment according to the requirement.
Advantageous effects
The invention has the beneficial effects that:
1. the microbial bacteriostatic deodorant provided by the invention breaks through the form of the traditional bacteriostatic deodorant: the existing microbial smell-removing agent is very rare, the microbial agent is commonly used for wastewater treatment and the like, no report is found for removing peculiar smell in the environment, the microbial agent is usually in a liquid form, and substances to be treated in the environment are absorbed into the liquid and then are adsorbed or absorbed and decomposed by microbes in the liquid; the microbial inoculum in the form has the problems of inconvenient use, easy pollution and deterioration, short service life and high cost, and is not suitable for use in complex environments; the invention adopts the form of bacteriostatic odor-removing agent of solid microbial inoculum in a breakthrough way;
after the program drying step obtained by adopting a special algorithm, the moisture in the antibacterial deodorant is not completely removed, so that the activity of microorganisms is ensured, and meanwhile, due to the special parameter setting of the program drying step, the antibacterial deodorant can be well molded while the activity of the microorganisms is ensured; the novel microbial deodorant in a solid form can be suitable for various complex environments, is not easy to deteriorate and has long service life.
2. The microbial antibacterial deodorant disclosed by the invention adopts plant waste as a fermentation raw material, so that a large amount of cost is saved, and the microbial antibacterial deodorant is suitable for occasions using the deodorant on a large scale, such as farms.
3. The plant additive is added on the basis of microorganisms, and has the effects of inhibiting bacteria, removing odor and improving the quality of ambient air.
4. According to the invention, the porous surface layer is added on the surface of the antibacterial odor removing agent, and through special heat treatment, under the conditions that the odor removing agent absorbs water and the inner layer becomes soft, the surface layer can ensure that the particles of the odor removing agent are not scattered, and the surface layer is the porous surface layer, so that the gas exchange among water, microorganisms and the environment in the odor removing agent is not influenced.
5. In the antibacterial deodorant provided by the invention, the plant waste and the porous surface layer are of water-absorbing structures, and can continuously absorb water vapor in the environment, so that the activity of microorganisms in the plant waste and the porous surface layer is ensured for a long time.
Detailed Description
The embodiments of the present invention are described below with reference to specific embodiments, and other advantages and effects of the present invention will be easily understood by those skilled in the art from the disclosure of the present specification. The invention is capable of other and different embodiments and of being practiced or of being carried out in various ways, and its several details are capable of modification in various respects, all without departing from the spirit and scope of the present invention.
The following examples and comparative examples are parallel runs, with the same processing steps and parameters, unless otherwise indicated. The corresponding deposit numbers for the species referred to in the following examples are: enterobacter huoshanense CCTCC No. M2013695, Bacillus subtilis CCTCC No. M2012078, Bacillus sonorae CGMCC No.15824, Leuconostoc mesenteroides are Leuconostoc mesenteroides with the preservation number of CGMCC No.6432, Bacillus amylovorus CGMCC No.6153, Weissella Greeceflower GDMCC No.60377, provided by the university of Jiangnan of the Bacillus Oleronius BNCC161714, Pediococcus pentosaceus purchased from Shanghai Yaya Biotechnology Co., Ltd, the cargo number XY-WSW-1818, the rhamnosus CGMCC No.11849 and the Microbacterium thermophilus ATCC 7953.
Example 1 preparation of a microbial bacteriostatic odor-removing agent:
(1) preparing a microbial fermentation liquid: pulverizing plant waste material 15Sieving with 0 mesh sieve, sterilizing at 121 deg.C under high temperature and high pressure for 30min, adding compound microorganism solution prepared with sterile water, and mixing; in the compound microorganism bacterial liquid, the bacterial concentration of the enterobacter hopcalis is 8.18-9.85 multiplied by 109The cell density of the bacillus subtilis is 5.26-6.19 multiplied by 1010The cell concentration of the Bacillus sonoralis in the strain/mL is 4.18-6.99 multiplied by 108The cell concentration of Leuconostoc mesenteroides is 2.69-4.66 × 1010The cell concentration of Bacillus amylovorus per mL is 3.78-5.12 × 109The cell density of Weissella Greek is 6.19-8.24 × 108The cell concentration of the Bacillus Oleronius per mL is 2.69-5.49 × 109The cell density of Pediococcus pentosaceus is 3.19-4.98 × 1010The cell concentration of rhamnosus bacterium is 4.88-6.13 × 1010The cell concentration of the thermophilic microbacterium is 1.59-3.08X 108Per mL; the mass ratio of the plant waste to the composite microbial liquid is 3: 5; the plant waste is straw;
(2) and (3) microbial fermentation: the fermentation temperature is 25 ℃, and the fermentation time is 72 hours; intermittently aerating during fermentation for 1h every time, wherein the interval aeration time is 3 h;
(3) adding a plant additive into the fermentation product, uniformly mixing, and standing: plant additives including tobacco leaf, tea leaf residue, fructus Gleditsiae Abnormalis, pericarpium Citri Tangerinae, and Colophonium; wherein the mass ratio of the tobacco leaves to the tea leaves, the saponin to the orange peel to the rosin is 1:5:2:5: 1; the mass ratio of the plant additive to the fermentation product is 3: 10; standing at 20 deg.C for 1 hr;
(4) and (3) granulation: the grain diameter is 5mm, and the temperature of a granulator is 25 ℃;
(5) drying by a program;
(6) coating a porous surface layer on the surface of the dried particles;
(7) and (3) rapidly cooling after heat treatment to obtain the microbial antibacterial deodorant: the heat treatment temperature is 120 ℃, and the heat treatment time is 30 s; the temperature is rapidly reduced, and the temperature reduction rate is 150 ℃/min.
The procedure drying in the step (5) specifically comprises the following steps: and (3) preserving heat for a period of time after the first temperature rise, cooling, preserving heat after the second temperature rise, and naturally cooling to room temperature.
The above procedure drying step: the first temperature rise is carried out, the temperature rise rate is 2 ℃/min, the temperature is raised to 25 ℃, and the heat preservation time is 20min after the first temperature rise; the temperature is reduced, wherein the temperature reduction rate is 2 ℃/min, the temperature is reduced to 20 ℃, and the heat preservation time is 5 min; and finally, heating for the second time, wherein the heating rate is 2 ℃/min, the heating is carried out to 35 ℃, and the heat preservation time after the second heating is 2 min.
And (6) wrapping the porous surface layer, specifically: 1) preparing a surface layer wrapping liquid: preparing bamboo charcoal powder and quicklime powder into suspension with water; 2) wrapping the suspension on the surface of the dried particles obtained in the step (5); 3) drying to form a porous surface layer. Step 1), the mass ratio of the bamboo charcoal powder, the quicklime powder and the water is 8:1: 10; the wrapping method in the step 2) comprises the following steps: rolling the dried particles obtained in the step (5), and spraying the suspension prepared in the step 1) on the surfaces of the particles; step 2), the mass ratio of the suspension wrapped on the surfaces of the dried particles to the dried particles is 2: 7; and 3) drying, wherein the drying temperature is 30 ℃, and the drying time is 2 min.
Example 2 preparation of a microbial bacteriostatic odor-removing agent:
(1) preparing a microbial fermentation liquid: pulverizing the plant waste, sieving with 200 mesh sieve, sterilizing at 121 deg.C under high temperature and high pressure for 30min, adding compound microorganism solution prepared with sterile water, and mixing; in the compound microorganism bacterial liquid, the bacterial concentration of the enterobacter hopcalis is 8.18-9.85 multiplied by 109The cell density of the bacillus subtilis is 5.26-6.19 multiplied by 1010The cell concentration of the Bacillus sonoralis in the strain/mL is 4.18-6.99 multiplied by 108The cell concentration of Leuconostoc mesenteroides is 2.69-4.66 × 1010The cell concentration of Bacillus amylovorus per mL is 3.78-5.12 × 109The cell density of Weissella Greek is 6.19-8.24 × 108The cell concentration of the Bacillus Oleronius per mL is 2.69-5.49 × 109The cell density of Pediococcus pentosaceus is 3.19-4.98 × 1010The cell concentration of rhamnosus bacterium is 4.88-6.13 × 1010The cell concentration of the thermophilic microbacterium is 1.59-3.08X 108Per mL; the mass ratio of the plant waste to the composite microbial liquid is 5: 17; the plant waste is corncob;
(2) and (3) microbial fermentation: the fermentation temperature is 27 ℃, and the fermentation time is 120 h; intermittently aerating for 2h each time in the fermentation period, wherein the interval aeration time is 5 h;
(3) adding a plant additive into the fermentation product, uniformly mixing, and standing: plant additives including tobacco leaf, tea leaf residue, fructus Gleditsiae Abnormalis, pericarpium Citri Tangerinae, and Colophonium; wherein the mass ratio of the tobacco leaves to the tea leaves, the saponin to the orange peel to the rosin is 3:10:5:8: 2; the mass ratio of the plant additive to the fermentation product is 3: 80; standing at 25 deg.C for 2 hr;
(4) and (3) granulation: the grain diameter is 15mm, and the temperature of a granulator is 30 ℃;
(5) drying by a program;
(6) coating a porous surface layer on the surface of the dried particles;
(7) and (3) rapidly cooling after heat treatment to obtain the microbial antibacterial deodorant: the heat treatment temperature is 150 ℃, and the heat treatment time is 60 s; the temperature is rapidly reduced, and the temperature reduction rate is 200 ℃/min.
The procedure drying in the step (5) specifically comprises the following steps: and (3) preserving heat for a period of time after the first temperature rise, cooling, preserving heat after the second temperature rise, and naturally cooling to room temperature.
The above procedure drying step: the first temperature rise is carried out, the temperature rise rate is 5 ℃/min, the temperature rise is carried out to 30 ℃, and the heat preservation time is 50min after the first temperature rise; the temperature is reduced, wherein the temperature reduction rate is 5 ℃/min, the temperature is reduced to 23 ℃, and the heat preservation time is 10 min; and finally, heating for the second time at the heating rate of 5 ℃/min to 37 ℃, and keeping the temperature for 5min after the second heating.
And (6) wrapping the porous surface layer, specifically: 1) preparing a surface layer wrapping liquid: preparing bamboo charcoal powder and quicklime powder into suspension with water; 2) wrapping the suspension on the surface of the dried particles obtained in the step (5); 3) drying to form a porous surface layer. Step 1), the mass ratio of the bamboo charcoal powder, the quicklime powder and the water is 15:3: 25; the wrapping method in the step 2) comprises the following steps: rolling the dried particles obtained in the step (5), and spraying the suspension prepared in the step 1) on the surfaces of the particles; step 2) the mass ratio of the suspension wrapped on the surfaces of the dried particles to the dried particles is 10: 3; and 3) drying, wherein the drying temperature is 35 ℃, and the drying time is 10 min.
Example 3 preparation of a microbial bacteriostatic odor-removing agent:
(1) preparing a microbial fermentation liquid: pulverizing the plant waste, sieving with 150 mesh sieve, sterilizing at 121 deg.C under high temperature and high pressure for 30min, adding compound microorganism solution prepared with sterile water, and mixing; in the compound microorganism bacterial liquid, the bacterial concentration of the enterobacter hopcalis is 8.18-9.85 multiplied by 109The cell density of the bacillus subtilis is 5.26-6.19 multiplied by 1010The cell concentration of the Bacillus sonoralis in the strain/mL is 4.18-6.99 multiplied by 108The cell concentration of Leuconostoc mesenteroides is 2.69-4.66 × 1010The cell concentration of Bacillus amylovorus per mL is 3.78-5.12 × 109The cell density of Weissella Greek is 6.19-8.24 × 108The cell concentration of the Bacillus Oleronius per mL is 2.69-5.49 × 109The cell density of Pediococcus pentosaceus is 3.19-4.98 × 1010The cell concentration of rhamnosus bacterium is 4.88-6.13 × 1010The cell concentration of the thermophilic microbacterium is 1.59-3.08X 108Per mL; the mass ratio of the plant waste to the composite microbial liquid is 3: 5; the plant waste is straw;
(2) and (3) microbial fermentation: the fermentation temperature is 25 ℃, and the fermentation time is 72 hours; intermittently aerating during fermentation for 1h every time, wherein the interval aeration time is 3 h;
(3) adding a plant additive into the fermentation product, uniformly mixing, and standing: plant additives including tobacco leaf, tea leaf residue, fructus Gleditsiae Abnormalis, pericarpium Citri Tangerinae, and Colophonium; wherein the mass ratio of the tobacco leaves to the tea leaves, the saponin to the orange peel to the rosin is 1:5:2:5: 1; the mass ratio of the plant additive to the fermentation product is 3: 10; standing at 20 deg.C for 1 hr;
(4) and (3) granulation: the grain diameter is 5mm, and the temperature of a granulator is 25 ℃;
(5) drying by a program;
(6) coating a porous surface layer on the surface of the dried particles;
(7) and (3) rapidly cooling after heat treatment to obtain the microbial antibacterial deodorant: the heat treatment temperature is 120 ℃, and the heat treatment time is 30 s; the temperature is rapidly reduced, and the temperature reduction rate is 150 ℃/min.
The procedure drying in the step (5) specifically comprises the following steps: and (3) preserving heat for a period of time after the first temperature rise, cooling, preserving heat after the second temperature rise, and naturally cooling to room temperature.
The above procedure drying step: the first temperature rise is carried out, the temperature rise rate is 2 ℃/min, the temperature is raised to 25 ℃, and the heat preservation time is 20min after the first temperature rise; cooling at a rate of 2 ℃/min to 20 ℃ and keeping the temperature for a period of time; finally, heating for the second time, wherein the heating rate is 2 ℃/min, the heating is carried out to 35 ℃, and the heat preservation time is 2min after the second heating; in temperature programming/cooling, the relationship between the holding time is as follows:
wherein, tjFor the holding time after cooling, ts1M is the holding time after the first temperature risejM is the mass of the complex microbial liquidfMass m of the plant wastezIs the quality of the plant additive.
And (6) wrapping the porous surface layer, specifically: 1) preparing a surface layer wrapping liquid: preparing bamboo charcoal powder and quicklime powder into suspension with water; 2) wrapping the suspension on the surface of the dried particles obtained in the step (5); 3) drying to form a porous surface layer. Step 1), the mass ratio of the bamboo charcoal powder, the quicklime powder and the water is 8:1: 10; the wrapping method in the step 2) comprises the following steps: rolling the dried particles obtained in the step (5), and spraying the suspension prepared in the step 1) on the surfaces of the particles; step 2), the mass ratio of the suspension wrapped on the surfaces of the dried particles to the dried particles is 2: 7; and 3) drying, wherein the drying temperature is 30 ℃, and the drying time is 2 min.
Example 4 application of the microbial bacteriostatic odor-removing agent prepared in examples 1 to 3 in removing odor in environment
The microbial bacteriostatic odor-removing agent prepared in the examples 1 to 3 is applied to a farm deodorization experiment and a dry toilet deodorization experiment, and the experimental method, the experimental environment and the detection data of the deodorizing effect of the microbial bacteriostatic odor-removing agent prepared in the example 3 are shown in the following table:
TABLE 1 odor detection inorganization exhaust gas detection result report of farm deodorization experiment
TABLE 2 odor detection inorganization exhaust gas detection result report of farm deodorization experiment
Table 3 plant deodorization experiment odor detection and detection basis summary table
Table 4 plant deodorization experiment odor detection and detection instrument list table
TABLE 5 odor detection inorganization exhaust gas detection point location coordinates for farm deodorization experiment
TABLE 6 statistical table of odor detection meteorological parameter observation results of plant deodorization experiment
TABLE 7 odor detection unstructured waste gas detection result report for dry latrine deodorization experiment
TABLE 8 odor detection unstructured waste gas detection result report for dry toilet deodorization experiment
Table 9 odor detection test basis summary table for dry toilet deodorization experiment
Table 10 odor detection and detection instrument list for dry toilet deodorization experiment
TABLE 11 odor detection inorganization waste gas detection point location coordinates for dry latrine deodorization experiment
TABLE 12 statistical table for odor detection meteorological parameter observation results of dry toilet deodorization experiment
Experiments show that the microbial inoculum prepared in the embodiment 1-3 has good deodorization effect, the deodorization rate of the embodiment 3 is fastest, the completeness of the bacteriostatic deodorant particles is best after recovery, and the difference of the integral deodorization effect in the embodiment 1-3 is not large. From the data in the table, the antibacterial deodorant prepared in the embodiment 3 can greatly reduce the odor concentration in the environment with high concentration of the odorous gas, including the concentration of hydrogen sulfide, ammonia and TVOC, in a short time, so that the environment is quickly optimized and comfortable; in addition, the antibacterial odor-removing agent can be directly placed at the corners of walls and other positions in the environment, and the problem that the liquid odor-removing agent is not easy to place is solved; the smell removing agent is easy to recover, and the effective use time is half a year to one year.
While the preferred embodiments and examples of the present invention have been described in detail, the present invention is not limited to the embodiments and examples, and various changes can be made without departing from the spirit of the present invention within the knowledge of those skilled in the art.
Claims (8)
1. A preparation method of a microbial bacteriostatic odor removing agent is characterized by comprising the following steps: the method comprises the following steps:
(1) preparing a microbial fermentation broth;
(2) fermenting by microorganisms;
(3) adding plant additives into the fermentation product, uniformly mixing, and standing;
(4) granulating;
(5) drying by a program;
(6) coating a porous surface layer on the surface of the dried particles;
(7) rapidly cooling after heat treatment to obtain a microbial bacteriostatic deodorant;
the microbial fermentation liquid obtained in the step (1) comprises: plant waste, complex microbial liquid; the plant waste is one or more than two of straw, wheat husk, rice husk and corncob; the compound microorganism liquid comprises enterobacter hopcalis, bacillus subtilis, bacillus sonoralis, leuconostoc mesenteroides, bacillus amylovorus, weissella schinensis, bacillus Oleronius, pediococcus pentosaceus, lactobacillus rhamnosus and microbacterium thermophilus; the preparation method of the microbial fermentation liquid comprises the following steps: crushing the plant waste, sieving with a sieve of 150-plus-200 meshes, sterilizing at 121 ℃ for 30min under high temperature and high pressure, adding a compound microbial solution prepared by sterile water, and uniformly mixing to obtain the compound microbial solution; the mass ratio of the plant waste to the composite microbial liquid is (1-30) to (5-50).
2. The method for preparing the microbe bacteriostatic odor-removing agent according to claim 1, wherein the method comprises the following steps: fermenting the microorganisms in the step (2), wherein the fermentation temperature is 25-27 ℃, and the fermentation time is 72-120 h; intermittently aerating for 1-2h each time during fermentation, and 3-5h at intervals.
3. The method for preparing the microbe bacteriostatic odor-removing agent according to claim 1, wherein the method comprises the following steps: the plant additive in the step (3) comprises tobacco leaves, tea leaves, Chinese honeylocust fruits, orange peels and turpentine; wherein the mass ratio of the tobacco leaves, the tea leaves, the Chinese honeylocust fruits, the orange peels and the turpentine is (1-3) to (5-10) to (2-5) to (5-8) to (1-2); the mass ratio of the plant additive to the fermentation product is (1-5) to (10-100); the standing temperature is 20-25 ℃, and the standing time is 1-2 h.
4. The method for preparing the microbe bacteriostatic odor-removing agent according to claim 1, wherein the method comprises the following steps: granulating in the step (4), wherein the particle size is 5-15mm, and the temperature of the granulator is 20-30 ℃.
5. The method for preparing the microbe bacteriostatic odor-removing agent according to claim 1, wherein the method comprises the following steps: the procedure drying in the step (5) specifically comprises the following steps: and (3) preserving heat for a period of time after the first temperature rise, cooling, preserving heat after the second temperature rise, and naturally cooling to room temperature.
6. The method for preparing the microbe bacteriostasis deodorant according to claim 5, which is characterized in that: the first temperature rise is carried out, the temperature rise rate is 2-5 ℃/min, the temperature is raised to 25-30 ℃, and the heat preservation time is 20-50min after the first temperature rise; cooling at a cooling rate of 2-5 ℃/min to 20-23 ℃ for 5-10 min; and finally, heating for the second time at the heating rate of 2-5 ℃/min to 35-37 ℃, and keeping the temperature for 2-5min after the second heating.
7. The method for preparing the microbe bacteriostatic odor-removing agent according to claim 1, wherein the method comprises the following steps: and (6) wrapping the porous surface layer, specifically: 1) preparing a surface layer wrapping liquid: preparing bamboo charcoal powder and quicklime powder into suspension with water; 2) wrapping the suspension on the surface of the dried particles obtained in the step (5); 3) drying to form a porous surface layer.
8. The method for preparing the microbe bacteriostatic odor-removing agent according to claim 1, wherein the method comprises the following steps: the heat treatment in the step (7) is carried out, wherein the heat treatment temperature is 120-150 ℃, and the heat treatment time is 30-60 s; the temperature is rapidly reduced at a rate of 150-.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010617315.6A CN111686287B (en) | 2020-06-30 | 2020-06-30 | Preparation method and application of microbial bacteriostatic deodorant |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010617315.6A CN111686287B (en) | 2020-06-30 | 2020-06-30 | Preparation method and application of microbial bacteriostatic deodorant |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN111686287A CN111686287A (en) | 2020-09-22 |
| CN111686287B true CN111686287B (en) | 2021-02-02 |
Family
ID=72484604
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202010617315.6A Active CN111686287B (en) | 2020-06-30 | 2020-06-30 | Preparation method and application of microbial bacteriostatic deodorant |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN111686287B (en) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112717186B (en) * | 2020-12-22 | 2023-02-14 | 新疆河润水业有限责任公司 | Microbial bacteriostatic odor-removing preparation and preparation method and application thereof |
| CN112501085B (en) * | 2020-12-22 | 2022-06-07 | 新疆河润水业有限责任公司 | Compound microbial agent applied to sewage treatment |
| CN112694997B (en) * | 2021-01-18 | 2024-02-06 | 新疆河润水业有限责任公司 | Composite microorganism bacterial stock solution suitable for sewage treatment and active liquid culture method |
| CN112662657B (en) * | 2021-01-18 | 2022-02-08 | 新疆河润水业有限责任公司 | Microbial agent, preparation method thereof and application thereof in sewage treatment |
| CN113599998A (en) * | 2021-07-20 | 2021-11-05 | 上海野马环保设备工程有限公司 | Preparation method of wet garbage compost biological deodorant |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111111423A (en) * | 2019-12-30 | 2020-05-08 | 井冈山市傲新华富育种有限公司 | Odor treatment spray for farm, and preparation method and application thereof |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102115699A (en) * | 2009-12-31 | 2011-07-06 | 上海泓宝绿色水产科技发展有限公司 | Preparation method and application of composite microbial enzyme product (descaling agent) for removing scale and odor of toilet bowl |
| CN102861350B (en) * | 2012-06-11 | 2014-04-30 | 中国科学院成都生物研究所 | Compound biological agent for deodorizing public toilets, and preparation method and application of compound biological agent |
| CN104152374A (en) * | 2014-07-10 | 2014-11-19 | 河南恒野农牧股份有限公司 | Microbial preparation for purifying breeding wastewater |
| CN107617331A (en) * | 2017-10-10 | 2018-01-23 | 默克环保科技（湖南）有限公司 | A kind of environment friendly biological deodorant and preparation method thereof |
| CN109279748A (en) * | 2018-09-25 | 2019-01-29 | 天津大学 | A kind of pig farm microbial deodorant and preparation method |
| KR102154930B1 (en) * | 2019-05-23 | 2020-09-10 | 최승국 | air purifying agent with excellent antibacterial and deodorizing activity |
-
2020
- 2020-06-30 CN CN202010617315.6A patent/CN111686287B/en active Active
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111111423A (en) * | 2019-12-30 | 2020-05-08 | 井冈山市傲新华富育种有限公司 | Odor treatment spray for farm, and preparation method and application thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN111686287A (en) | 2020-09-22 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN111686287B (en) | Preparation method and application of microbial bacteriostatic deodorant | |
| CN108251332A (en) | A kind of microbial deodorant and preparation method thereof | |
| CN107281928B (en) | A kind of environmental protection ferment and its preparation process with deodorization air-cleaning function | |
| CN102258796A (en) | Microbial deodorant and preparation method thereof | |
| KR102244422B1 (en) | Manufacturing method of natural deodorant containing complex microorganism and the natural deodorant prepared therefrom | |
| CN111876358A (en) | Livestock and poultry manure deodorization microbial agent and preparation method and application thereof | |
| CN102614539B (en) | Preparation method for active carbon deodorant and application of active carbon deodorant in removal of fish meal processing odor | |
| CN111012938A (en) | Deodorant for removing odor generated from garbage | |
| CN110894102A (en) | Method for purifying malodorous substances in livestock breeding environment | |
| CN105255761B (en) | A kind of deodorization microbial inoculum and its application method for organic waste treatment | |
| CN110438031A (en) | A kind of microbial deoderizer and preparation method thereof | |
| CN108611301B (en) | Composite biological deodorant and preparation method thereof | |
| CN108246094A (en) | A kind of composite highly effective liquid bio deodorant and preparation method thereof | |
| CN108479352B (en) | Domestic garbage deodorizing bacteriostatic agent and preparation method and treatment method thereof | |
| CN109092048A (en) | Microbiocidal deodorant | |
| CN111514740B (en) | Biological deodorant and preparation method thereof | |
| CN103656712A (en) | Seaweed biological deodorant and preparation method thereof | |
| KR101549191B1 (en) | Material treating for reduction of malodor gas | |
| CN112717186B (en) | Microbial bacteriostatic odor-removing preparation and preparation method and application thereof | |
| CN110026060A (en) | A kind of biomaterial and its making and use method of degradation of formaldehyde | |
| CN107522371B (en) | Livestock and poultry breeding waste treatment method capable of reducing heavy metal and pathogenic bacteria | |
| CN112191085B (en) | Preparation method of garbage deodorant | |
| CN108993130A (en) | A kind of method of pig manure stink degradation | |
| CN107754579A (en) | A kind of exhaust treatment system and processing method for plant | |
| CN114149944A (en) | Microorganism combination with tail end capable of efficiently adsorbing malodorous gas, enhanced colonization and application |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |