CN111587894A - Ecological moisturizing and sand-fixing vegetation growth promoter and preparation method thereof - Google Patents
Ecological moisturizing and sand-fixing vegetation growth promoter and preparation method thereof Download PDFInfo
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- A—HUMAN NECESSITIES
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Abstract
An ecological moisturizing and sand-fixing vegetation growth promoter and a preparation method thereof. The invention discloses an ecological sand-fixation vegetation growth promoter which comprises the following raw material components in parts by weight: 20-30 parts of compound sodium nitrophenolate, 15-20 parts of chitosan, 15-20 parts of humic acid, 10-15 parts of derris root powder, 2-5 parts of bacillus subtilis and 2-5 parts of bacillus licheniformis. The invention also discloses a preparation method of the growth promoter, which comprises the following steps: s1, weighing the raw material components in parts by weight respectively; s2, uniformly mixing the humic acid and the compound sodium nitrophenolate weighed in the S1 to obtain a mixture A; s3, uniformly mixing the chitosan weighed in the S1 and the derris root powder to obtain a mixture B; and S4, respectively culturing the microbial bacteria, mixing, adding the mixture A and the mixture B, fermenting and drying to obtain the growth promoter. The vegetation growth promoter provided by the invention promotes the growth of vegetation through the synergistic effect of the components, and improves the transplanting survival rate and the growth rate of vegetation.
Description
Technical Field
The invention belongs to the technical field of water-retaining agents, and particularly relates to an ecological moisturizing and sand-fixing vegetation growth promoter and a preparation method thereof.
Background
At present, the main methods for desert control at home and abroad comprise biological sand fixation, physical sand fixation and chemical sand fixation. The physical sand fixation is that barriers or structures are arranged at the edge of the desert to prevent the moving sand dunes from moving, the physical sand fixation mode has high cost and can only play a role in a short distance, and the moving sand is submerged and filled in less than 2-5 years due to the fact that the moving sand is just below the city and the sand barriers with the height of tens of centimeters are submerged, so that the effective time is short; the chemical sand fixation at the present stage is difficult to popularize and apply on a large scale because the cost is high, the environmental adaptability is poor, and some sand fixation materials cause secondary pollution to the environment; biological sand fixation is also called plant sand fixation, is a technical measure for preventing and controlling deserts, stabilizing oasis, improving the environmental quality and production potential of sand areas by means of sealing and planting plants and the like, is the most effective way at present for desert control, but in desertification areas, due to the limitation of growth environment, the survival rate of plants is not very high, so the development of plant desert control is limited.
Disclosure of Invention
In order to overcome the defects of the prior art, the invention aims to provide an ecological moisturizing and sand-fixing vegetation growth promoter and a preparation method thereof.
The invention aims to provide an ecological moisturizing and sand-fixing vegetation growth promoter which comprises the following raw material components in parts by weight: 20-30 parts of compound sodium nitrophenolate, 15-20 parts of chitosan, 15-20 parts of humic acid, 10-15 parts of derris root powder, 2-5 parts of bacillus subtilis and 2-5 parts of bacillus licheniformis.
Preferably, the ecological moisturizing and sand-fixing vegetation growth promoter provided by the invention comprises the following raw material components in parts by weight: 25 parts of compound sodium nitrophenolate, 20 parts of chitosan, 15 parts of humic acid, 10 parts of derris root powder, 5 parts of bacillus subtilis and 5 parts of bacillus licheniformis.
Preferably, the effective viable count of both bacillus subtilis and bacillus licheniformis is 103～105CFU/g。
Preferably, the derris root powder is prepared by the following steps:
crushing the dried derris roots to 100-200 meshes, adding 90% organic solvent water solution, soaking and extracting for 3-5 times, each time for 2-4 hours, filtering, combining filtrates, concentrating, and spray drying to obtain derris root powder, wherein the volume ratio of the derris roots to the organic solvent is 1: 4-8.
Further preferably, the organic solvent is a natural turpentine derivative, and the turpentine derivative is an isomerization and disproportionation product of pinene.
The second purpose of the invention is to provide a preparation method of the ecological moisturizing and sand-fixing vegetation growth promoter, which comprises the following steps:
s1, weighing 20-30 parts of compound sodium nitrophenolate, 15-20 parts of chitosan, 15-20 parts of humic acid, 10-15 parts of derris root powder, 2-5 parts of bacillus subtilis and 2-5 parts of bacillus licheniformis according to parts by weight;
s2, uniformly mixing the humic acid and the compound sodium nitrophenolate weighed in the S1 to obtain a mixture A;
s3, uniformly mixing the chitosan weighed in the S1 and the derris root powder to obtain a mixture B;
s4, respectively propagating the bacillus subtilis and the bacillus licheniformis weighed in the S1 in liquid culture media at the temperature of 20-30 ℃, and when the number of bacterial colonies in each liquid culture medium reaches 103～105And (3) mixing to obtain a mixed bacterial liquid when the concentration of the carbon dioxide is CFU/g, sequentially adding the mixture A obtained in the step S2 and the mixture B obtained in the step S3, fermenting for 16-24 hours, and drying to obtain the ecological moisturizing and sand-fixing vegetation growth promoter.
Preferably, in step S4, the liquid culture medium is a potato dextrose medium.
Compared with the prior art, the invention has the beneficial effects that:
the ecological moisturizing and sand-fixing vegetation growth promoter provided by the invention can promote the growth of plants to be healthy but not vigorous through the compound sodium nitrophenolate and the humic acid, not only increases the synthesis of total nutrients, but also coordinates the distribution relationship of vegetative growth and reproductive growth, not only increases the height of plants, but also thickens and strengthens stems; the derris root powder contains a large amount of amino acids, biological potassium and fatty acid, can be quickly absorbed by plants and can supplement plant nutrient elements, chitosan can improve the permeability of soil and is combined with the soil to form a larger granular structure, humic acid is further connected with the formed granular structure soil to form a stable soil structure, the chitosan and the derris root powder are combined for use, the using effect of compound sodium nitrophenolate can be improved, the bacillus subtilis and the bacillus licheniformis can be promoted to grow better, a stable and quick growth environment can be provided for microorganisms around the plant rhizosphere, the microorganisms can be quickly propagated, a nitrogen source is provided for the plants in turn, meanwhile, metabolites of the microorganisms generate plant growth hormones such as indoleacetic acid, gibberellin, polypeptides and the like, and the growth of the plant root system is further promoted. Therefore, the vegetation growth promoter provided by the invention promotes the growth of vegetation through the synergistic effect among the components, and improves the transplanting survival rate and the growth rate of the vegetation; meanwhile, when the growth promoter provided by the invention is used, the powder can be directly scattered on the plant rhizosphere and the surrounding soil, and can also be diluted by water and then applied on the plant rhizosphere and the surrounding soil.
Detailed Description
In order to make the technical solutions of the present invention better understood and enable those skilled in the art to practice the present invention, the following embodiments are further described, but the present invention is not limited to the following embodiments.
The test methods not specifically described in the following examples were carried out according to conventional methods and conditions in the art, and the starting materials were commercially available.
In the following examples, the organic solvent is a natural turpentine derivative, which is an isomeric disproportionation product of pinene; in step S4, all the liquid media used were potato dextrose media.
The bacillus subtilis and the bacillus licheniformis in the invention are both the existing strains of a microorganism strain preservation management center, do not relate to the development of new strains, and only relate to the application thereof. The bacillus subtilis used in the following embodiments is specifically a strain with a preservation number of ACCC 19743 of the China agricultural microbial culture Collection center (ACCC for short), and the bacillus licheniformis is specifically a strain with a preservation number of CGMCC7388 of the China general microbiological culture Collection center (CGMCC for short).
Example 1
The invention provides an ecological moisturizing and sand-fixing vegetation growth promoter which is composed of the following raw material components in parts by weight: 25 parts of compound sodium nitrophenolate, 20 parts of chitosan, 15 parts of humic acid, 10 parts of sargentgloryvine root powder, 5 parts of bacillus subtilis and 5 parts of bacillus licheniformis; the effective viable count of the bacillus subtilis and the bacillus licheniformis is 105CFU/g。
The derris root powder is prepared by the following steps:
pulverizing dried derris root to 100 meshes, adding 90% organic solvent water solution, soaking and extracting for 3 times, each time for 3h, filtering, combining filtrates, concentrating, and spray drying to obtain derris root powder, wherein the volume ratio of derris root to organic solvent is 1: 4.
The preparation method of the ecological moisturizing and sand-fixing vegetation growth promoter specifically comprises the following steps:
s1, respectively weighing 25 parts of compound sodium nitrophenolate, 20 parts of chitosan, 15 parts of humic acid, 10 parts of sargentgloryvine root powder, 5 parts of bacillus subtilis and 5 parts of bacillus licheniformis in parts by weight;
s2, uniformly mixing the humic acid and the compound sodium nitrophenolate weighed in the S1 to obtain a mixture A;
s3, uniformly mixing the chitosan weighed in the S1 and the derris root powder to obtain a mixture B;
s4, weighing the bacillus subtilis and the lichen bud in the S1Respectively propagating the bacillus in liquid culture medium at 20 deg.C, when the colony number in each liquid culture medium reaches 105And (3) mixing to obtain a mixed bacterial liquid when the concentration of the carbon black is CFU/g, sequentially adding the mixture A obtained in the step S2 and the mixture B obtained in the step S3, fermenting for 20 hours, and drying to obtain the ecological moisturizing and sand-fixing vegetation growth promoter.
Example 2
The invention provides an ecological moisturizing and sand-fixing vegetation growth promoter which is composed of the following raw material components in parts by weight: 30 parts of compound sodium nitrophenolate, 15 parts of chitosan, 20 parts of humic acid, 12 parts of sargentgloryvine root powder, 2 parts of bacillus subtilis and 2 parts of bacillus licheniformis; the effective viable count of the bacillus subtilis and the bacillus licheniformis is 103CFU/g。
The derris root powder is prepared by the following steps:
pulverizing dried derris root to 200 meshes, adding 90% organic solvent water solution, soaking and extracting for 5 times, each time for 2h, filtering, combining filtrates, concentrating, and spray drying to obtain derris root powder, wherein the volume ratio of derris root to organic solvent is 1: 8.
The preparation method of the ecological moisturizing and sand-fixing vegetation growth promoter specifically comprises the following steps:
s1, weighing 30 parts of compound sodium nitrophenolate, 15 parts of chitosan, 20 parts of humic acid, 12 parts of sargentgloryvine root powder, 2 parts of bacillus subtilis and 2 parts of bacillus licheniformis according to parts by weight;
s2, uniformly mixing the humic acid and the compound sodium nitrophenolate weighed in the S1 to obtain a mixture A;
s3, uniformly mixing the chitosan weighed in the S1 and the derris root powder to obtain a mixture B;
s4, respectively propagating the bacillus subtilis and the bacillus licheniformis weighed in the S1 in liquid culture media at the temperature of 30 ℃, and when the colony number in each liquid culture medium reaches 103And (3) mixing to obtain a mixed bacterial liquid when the concentration of the carbon black is CFU/g, sequentially adding the mixture A obtained in the step S2 and the mixture B obtained in the step S3, fermenting for 16 hours, and drying to obtain the ecological moisturizing and sand-fixing vegetation growth promoter.
Example 3
Hair brushThe ecological moisturizing and sand-fixing vegetation growth promoter provided by the invention specifically comprises the following raw material components in parts by weight: 25 parts of compound sodium nitrophenolate, 18 parts of chitosan, 18 parts of humic acid, 15 parts of sargentgloryvine root powder, 3 parts of bacillus subtilis and 2 parts of bacillus licheniformis; the effective viable count of the bacillus subtilis and the bacillus licheniformis is 104CFU/g。
The derris root powder is prepared by the following steps:
pulverizing dried derris root to 150 meshes, adding 90% organic solvent water solution, soaking and extracting for 4 times, each time for 4h, filtering, combining filtrates, concentrating, and spray drying to obtain derris root powder, wherein the volume ratio of derris root to organic solvent is 1: 6.
The preparation method of the ecological moisturizing and sand-fixing vegetation growth promoter specifically comprises the following steps:
s1, respectively weighing 25 parts of compound sodium nitrophenolate, 18 parts of chitosan, 18 parts of humic acid, 15 parts of sargentgloryvine root powder, 3 parts of bacillus subtilis and 2 parts of bacillus licheniformis in parts by weight;
s2, uniformly mixing the humic acid and the compound sodium nitrophenolate weighed in the S1 to obtain a mixture A;
s3, uniformly mixing the chitosan weighed in the S1 and the derris root powder to obtain a mixture B;
s4, respectively propagating the bacillus subtilis and the bacillus licheniformis weighed in the S1 in liquid culture media at 25 ℃, and when the colony number in each liquid culture medium reaches 104And (3) mixing to obtain a mixed bacterial liquid when the concentration of the carbon black is CFU/g, sequentially adding the mixture A obtained in the step S2 and the mixture B obtained in the step S3, fermenting for 24 hours, and drying to obtain the ecological moisturizing and sand-fixing vegetation growth promoter.
Comparative example 1
Comparative example 1 the components of the ecological moisturizing and sand-fixing vegetation growth promoting agent provided in example 1 and the preparation method are the same, except that the components of the growth promoting agent provided in comparative example 1 do not contain bacillus subtilis.
Comparative example 2
Comparative example 2 the components of the ecological moisturizing and sand-fixing vegetation growth promoting agent provided in example 1 and the preparation method are the same, except that bacillus licheniformis is not contained in the components of the growth promoting agent provided in comparative example 2.
Comparative example 3
Comparative example 3 is the same as the components and preparation method of the ecological moisturizing and sand-fixing vegetation growth promoter provided in example 1, except that the components of the growth promoter provided in comparative example 3 do not contain derris root powder.
Comparative example 4
Comparative example 4 is the same as the components and preparation method of the ecological moisturizing and sand-fixing vegetation growth promoter provided in example 1, except that the components of the growth promoter provided in comparative example 4 do not contain chitosan.
The ecological moisturizing and sand-fixing vegetation growth promoting agents provided in the embodiments 1 to 3 can play a good role in promoting the growth of vegetation, so the effect of the invention is illustrated by only adopting the ecological moisturizing and sand-fixing vegetation growth promoting agent prepared in the embodiment 1.
The ecological moisturizing and sand-fixing vegetation growth promoting agents prepared in the example 1 and the comparative examples 1 to 4 are taken as examples, and the prepared growth promoting agents are subjected to comparative tests.
Test 1
Five desert lands (each mu) in the same geographical conditions in the area A-E are selected for comparison tests, wherein 1Kg of the growth promoting agent prepared in the example 1 is applied to the land in the area A, 1Kg of the growth promoting agent prepared in the comparative examples 1-4 is sequentially applied to the land in the area B-E, various vegetation seeds are sown in the five area A-E, and other management is similar to conventional management and cultivation.
Results after 15 days show that compared with four areas B-E, the area A is easy to grow various vegetation, the vegetation quickly grows into small seedlings after 1 month, and the root systems of the small seedlings are thick; and the four areas B-E have little change and no vegetation.
Test 2
Selecting five desert lands (each mu) with the same regional conditions in the A-E areas for comparison test, wherein 1Kg of the growth promoting agent prepared in example 1 and comparative examples 1-4 is sequentially applied to the four desert lands in the A-E areas respectively, transplanting various vegetations in the five desert lands in the A-E areas, watering enough water after transplanting, and managing and cultivating according to the conventional method.
The investigation result after 30 days of transplantation shows that the transplanting survival rate of the vegetation in the A block area reaches 99.7 percent, while the transplanting survival rate of the vegetation in the B-E four-block area is only 32.1 percent, 29.8 percent, 34.5 percent and 31.6 percent respectively.
The results of the above comparative tests show that the effect of the ecological moisturizing and sand-fixing vegetation growth promoting agent provided in example 1 of the present invention is superior to that of comparative examples 1 to 4, that is, the ecological moisturizing and sand-fixing vegetation growth promoting agent provided in example 1 of the present invention has a more excellent plant growth promoting function, and at the same time, it is demonstrated that the growth promoting agent provided in the present invention has the advantages of all components being deficient, all components being interacted, and functioning together.
In conclusion, the vegetation growth promoter provided by the embodiment of the invention promotes the growth of vegetation through the synergistic effect of the components, and improves the transplanting survival rate and the growth rate of the vegetation.
It should be noted that when the following claims refer to numerical ranges, it should be understood that both ends of each numerical range and any value between the two ends can be selected, and since the steps and methods used are the same as those of the embodiments, the preferred embodiments and effects thereof are described in the present invention for the sake of avoiding redundancy, but once the basic inventive concept is known, those skilled in the art may make other changes and modifications to the embodiments. Therefore, it is intended that the appended claims be interpreted as including preferred embodiments and all such alterations and modifications as fall within the scope of the invention.
It will be apparent to those skilled in the art that various changes and modifications may be made in the present invention without departing from the spirit and scope of the invention. Thus, if such modifications and variations of the present invention fall within the scope of the claims of the present invention and their equivalents, the present invention is also intended to include such modifications and variations.
Claims (6)
1. The ecological moisturizing and sand-fixing vegetation growth promoter is characterized by comprising the following raw material components in parts by weight: 20-30 parts of compound sodium nitrophenolate, 15-20 parts of chitosan, 15-20 parts of humic acid, 10-15 parts of derris root powder, 2-5 parts of bacillus subtilis and 2-5 parts of bacillus licheniformis;
said hayThe effective viable count of bacillus and bacillus licheniformis is 103～105CFU/g。
2. The ecological moisturizing and sand-fixing vegetation growth promoter according to claim 1, which is characterized by comprising the following raw material components in parts by weight: 25 parts of compound sodium nitrophenolate, 20 parts of chitosan, 15 parts of humic acid, 10 parts of derris root powder, 5 parts of bacillus subtilis and 5 parts of bacillus licheniformis.
3. The ecological moisturizing and sand-fixing vegetation growth promoter according to claim 1 or 2, wherein the derris root powder is prepared by the following steps:
crushing the dried derris roots to 100-200 meshes, adding 90% organic solvent water solution, soaking and extracting for 3-5 times, each time for 2-4 hours, filtering, combining filtrates, concentrating, and spray drying to obtain derris root powder, wherein the volume ratio of the derris roots to the organic solvent is 1: 4-8.
4. The ecological moisturizing and sand-fixing vegetation growth promoter as claimed in claim 3, wherein the organic solvent is a natural turpentine derivative, and the turpentine derivative is an isomeric and disproportionated product of pinene.
5. The method for preparing the vegetation growth promoter for ecological moisturizing and sand-fixing according to claim 1, which is characterized by comprising the following steps:
s1, weighing 20-30 parts of compound sodium nitrophenolate, 15-20 parts of chitosan, 15-20 parts of humic acid, 10-15 parts of derris root powder, 2-5 parts of bacillus subtilis and 2-5 parts of bacillus licheniformis according to parts by weight;
s2, uniformly mixing the humic acid and the compound sodium nitrophenolate weighed in the S1 to obtain a mixture A;
s3, uniformly mixing the chitosan weighed in the S1 and the derris root powder to obtain a mixture B;
s4, respectively propagating the bacillus subtilis and the bacillus licheniformis weighed in the S1 in liquid culture media at the temperature of 20-30 ℃, and performing strain propagation in each liquid culture mediumThe falling quantity reaches 103～105And (3) mixing to obtain a mixed bacterial liquid when the concentration of the carbon dioxide is CFU/g, sequentially adding the mixture A obtained in the step S2 and the mixture B obtained in the step S3, fermenting for 16-24 hours, and drying to obtain the ecological moisturizing and sand-fixing vegetation growth promoter.
6. The method for preparing the vegetation growth promoter for ecological moisture retention and sand fixation according to claim 5, wherein in step S4, the liquid culture medium is a potato dextrose culture medium.
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| CN110178557A (en) * | 2019-03-19 | 2019-08-30 | 福建农业职业技术学院 | A kind of Intermediate Gray woods layer in the solid native forest belt of three layers of ecological sand prevention and its method of growing into forest |
| CN110724008A (en) * | 2019-11-28 | 2020-01-24 | 吉林省嘉博生物科技有限公司 | Long-acting microbial agent for planting vegetation for slope treatment |
-
2020
- 2020-04-15 CN CN202010295107.9A patent/CN111587894B/en not_active Expired - Fee Related
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107189789A (en) * | 2017-06-28 | 2017-09-22 | 常州新之雅装饰材料有限公司 | A kind of ecological environment sand fixation material and preparation method thereof |
| CN108298893A (en) * | 2018-02-05 | 2018-07-20 | 湖州中煤地质工程有限公司 | A kind of Slope greening construction method and Slope greening matrix concrete |
| CN108383590A (en) * | 2018-03-15 | 2018-08-10 | 广西植被混凝土有限公司 | Rapid-hardening plant cover concrete weak acid afforests activating agent, its manufacturing method and its application |
| CN110178557A (en) * | 2019-03-19 | 2019-08-30 | 福建农业职业技术学院 | A kind of Intermediate Gray woods layer in the solid native forest belt of three layers of ecological sand prevention and its method of growing into forest |
| CN110079331A (en) * | 2019-03-28 | 2019-08-02 | 内蒙古自治区林业科学研究院 | A kind of preparation of water-soluble environment protective sand-consolidating agent and application method |
| CN110724008A (en) * | 2019-11-28 | 2020-01-24 | 吉林省嘉博生物科技有限公司 | Long-acting microbial agent for planting vegetation for slope treatment |
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| CN111587894B (en) | 2021-12-07 |
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