CN111548973A - Culture method of strain and application of strain in water treatment - Google Patents
Culture method of strain and application of strain in water treatment Download PDFInfo
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
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- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/348—Biological treatment of water, waste water, or sewage characterised by the microorganisms used characterised by the way or the form in which the microorganisms are added or dosed
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- C12N1/38—Chemical stimulation of growth or activity by addition of chemical compounds which are not essential growth factors; Stimulation of growth by removal of a chemical compound
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- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2209/00—Controlling or monitoring parameters in water treatment
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
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- C02F2305/00—Use of specific compounds during water treatment
- C02F2305/06—Nutrients for stimulating the growth of microorganisms
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Abstract
The invention discloses a culture method of a strain and application thereof in water treatment, belonging to the field of environment-friendly water treatment, and the culture method of the strain comprises the following steps: the method comprises the following steps: crushing and filtering soybean residue, taking filtered water, retaining filter residue, adding a porous material into the filtered water for impregnation, wherein the impregnated porous material is used as a carrier; step two: adding clear water into the carrier in the step one, then adding a biochemical microbial inoculum, and culturing to obtain a strain and a biochemical liquid containing the strain.
Description
Technical Field
The invention relates to the technical field of strain cultivation, in particular to a strain cultivation method and application thereof in water treatment.
Background
The water pollution condition of China is very serious, and investigation shows that more than 50 percent of rivers and more than 75 percent of lakes in China are polluted, the eutrophication phenomenon of water bodies occurs, and even black and odorous water bodies are formed. Particularly, under the social background that the industrial and urbanization process is accelerated, a large amount of industrial wastewater and untreated urban domestic sewage are discharged randomly, the water quality of surface and underground water bodies is polluted seriously, and the water environment safety condition of China is not optimistic. The urban sewage treatment plants mostly treat domestic sewage by taking a biological method as a core process, pollutants in water are mainly degraded or removed by microorganisms in the biological method treatment process, the quantity and activity of water treatment microorganisms are changed due to the temperature, the sewage treatment effect is obviously influenced, and particularly in cold regions in the north of China, seasonal low temperature can generate great impact on the sewage treatment process.
Disclosure of Invention
The invention aims to solve at least one of the technical problems in the prior art and provides a culture method of a strain and application thereof in water treatment.
The technical solution of the invention is as follows:
a method for culturing strains comprises the following steps:
the method comprises the following steps: crushing and filtering soybean residue, taking filtered water, retaining filter residue, adding a porous material into the filtered water for impregnation, wherein the impregnated porous material is used as a carrier;
step two: and (3) adding clear water into the carrier obtained in the step one, then adding a biochemical microbial inoculum, and culturing to obtain a strain and a biochemical liquid containing the strain.
Preferably, in the first step, the porous material is one or more of activated carbon, vermiculite and manganese sand.
Preferably, in the step one, the specific process of dipping is ultrasonic dipping for 5-9 h.
Preferably, in the second step, the specific process of culturing is as follows: performing first-stage aeration circulation at 10-25 deg.C, adding nutrient solution, performing second-stage aeration circulation, adding the residue, and performing third-stage aeration circulation at 5-10 deg.C.
Preferably, in the second step, the first stage of aeration cycle is 1-4 days, wherein the dissolved oxygen is 3-10 mg/L; in the second stage, aeration circulation is performed for 3-5 days, wherein the dissolved oxygen is 1-5 mg/L; in the third stage, aeration cycle is performed for 1-3 days, wherein the dissolved oxygen is 0.5-3 mg/L.
Preferably, in the second step, the nutrient solution is glucose.
Preferably, in the second step, the biochemical agents include denitrifying bacteria, nitrifying bacteria, bacillus and Streptococcus.
Preferably, in the second step, the addition amount of the biological agent is 0.5-3% of the mass of the clean water.
Another object of the present invention is to propose: the application of the culture method of the strain in water treatment is to mix the biochemical liquid obtained by any one of the culture methods with industrial wastewater, and then continuously add the strain to treat the industrial wastewater.
Preferably, the temperature of the industrial wastewater is 5-15 ℃.
The invention has at least one of the following beneficial effects:
(1) according to the method for cultivating the strain, the soybean residue is soaked in the porous material, so that on one hand, a nitrogen source and a carbon source required in the culture process are adsorbed in pores of the porous material in advance to facilitate subsequent culture of the strain, and on the other hand, the soybean residue contains rich iron and is adsorbed in gaps of the porous material, so that the iron can stimulate the strain, and the activity and the microbial quantity of the strain are improved.
(2) According to the method for cultivating the strains, disclosed by the invention, due to the fact that the porous material containing the manganese sand is adopted, the manganese sand can release a small amount of manganese ions, and the manganese element is one of trace elements essential for growth and reproduction of microorganisms, so that the manganese sand is applied to the field of sewage treatment, the growth and reproduction of the microorganisms in a treatment system can be stimulated, and a positive effect on improving the biochemical treatment effect is achieved.
(3) The culture method of the strain is applied to water treatment, industrial sewage in a low-temperature environment is treated by using the strain containing the culture and biochemical water containing the strain, and the sewage treatment effect is excellent.
Detailed Description
A method for culturing strains comprises the following steps:
the method comprises the following steps: crushing and filtering soybean residue, taking filtered water, retaining filter residue, adding a porous material into the filtered water for impregnation, wherein the impregnated porous material is used as a carrier;
step two: and (3) adding clear water into the carrier obtained in the step one, then adding a biochemical microbial inoculum, and culturing to obtain a strain and a biochemical liquid containing the strain.
In the first step, the porous material is one or more of activated carbon, vermiculite and manganese sand.
In the first step, the specific process of impregnation is ultrasonic impregnation for 5-9h, and the ultrasonic impregnation can disperse the filtered water more uniformly and evenly in each pore diameter in the porous material.
In the second step, the specific process of culturing is as follows: performing first-stage aeration circulation at 10-25 deg.C, adding nutrient solution, performing second-stage aeration circulation, adding the residue, and performing third-stage aeration circulation at 5-10 deg.C.
In the second step, the closed aeration circulation in the first stage is carried out for 1 to 4 days, wherein the dissolved oxygen is 3 to 10 mg/L; in the second stage, aeration circulation is performed for 3-5 days, wherein the dissolved oxygen is 1-5 mg/L; in the third stage, aeration cycle is performed for 1-3 days, wherein the dissolved oxygen is 0.5-3 mg/L.
In the second step, the nutrient solution is preferably glucose.
In the second step, the biochemical bacteria agent comprises denitrifying bacteria, nitrifying bacteria, bacillus and staphylococcus. The biological agent can be purchased from China general microbiological culture Collection center (CGMCC), China center for type culture Collection, American Type Culture Collection (ATCC) and China center for agricultural culture Collection (ACCC).
The existence form of the solid auxiliary material is preferably freeze-dried powder, microbial bacteria liquid or a mixture of the microbial bacteria liquid and the solid auxiliary material, and the freeze-dried powder is adopted in the following embodiments.
Wherein the denitrifying bacteria is Pseudomonas (Pseudomonas sp.N200), and the preservation number is as follows: CGMCC No. 18072; can also be Pseudomonas aeruginosa (Pseudomonas aeruginosa) G16X-D with the preservation number of CGMCC NO. 13108.
The nitrifying bacteria are nitrosomonas, and the preservation number is CCTCC NO: m2018813, can also be an heterotrophic nitrifying bacterium, which is called Hadoop heterotrophic nitrification Acinetobacter L7(Acinetobacter heterotrophic bacteria L7), and the preservation number is CGMCC No. 7486.
The bacillus may be bacillus cereus (bacillus cereus), with the deposit number: CGMCCNO.3836; the bacillus circulans can also be bacillus circulans (Bacillus circulans): CGMCC 1.554;
the Streptococcus chelidoni is TAD1(Chelatococcus daeguensis), and the preservation number is CGMCC No. 5226.
In the second step, the addition amount of the biological agent is 0.5-3% of the mass of the clean water.
Another object of the present invention is to propose: the application of the culture method of the strain in water treatment is to mix the biochemical liquid obtained by any one of the culture methods with industrial wastewater, and then continuously add the strain to treat the industrial wastewater. In particular the industrial wastewater treatment may be conventional prior art oxidation ditch technology.
The temperature of the industrial wastewater is 5-15 ℃.
In order to make the objects, technical solutions and advantages of the present invention more apparent, the following detailed description of the embodiments of the present invention is provided to facilitate understanding.
Example 1
A method for culturing strains comprises the following steps:
the method comprises the following steps: crushing and filtering soybean residue, taking filtered water, retaining filter residue, adding a porous material into the filtered water, and soaking for 5 hours, wherein the soaked porous material is used as a carrier; the addition amount of the porous material is 30 percent of the mass of the filtered water; the porous material is activated carbon.
Step two: adding clear water into the carrier obtained in the step one, and then adding a biochemical microbial inoculum accounting for 0.5% of the mass of the clear water for culturing to obtain a strain and a biochemical liquid containing the strain; the biochemical bacteria agent comprises the following components in percentage by mass: 1: 3: 5 pseudomonas, nitrobacter iso-oxygen, bacillus cereus, and TAD 1;
in the second step, the specific process of culturing is as follows: performing a first stage aeration cycle at 25 deg.C for 4 days with dissolved oxygen of 10mg/L, adding nutrient solution, and performing a second stage aeration cycle for 5 days with dissolved oxygen of 5 mg/L; then adding the filter residue obtained in the first step, and performing a third-stage closed aeration circulation at the temperature of 10 ℃ for 3 days, wherein the dissolved oxygen is 3 mg/L; the nutrient solution is glucose.
Example 2
A method for culturing strains comprises the following steps:
the method comprises the following steps: crushing and filtering soybean residue, taking filtered water, retaining filter residue, adding a porous material into the filtered water, and soaking for 7 hours, wherein the soaked porous material is used as a carrier; the addition amount of the porous material is 50 percent of the mass of the filtered water; the porous material is activated carbon.
Step two: adding clear water into the carrier obtained in the step one, and then adding a biochemical microbial inoculum accounting for 2% of the mass of the clear water for culturing to obtain a strain and a biochemical liquid containing the strain; the biochemical bacteria agent comprises the following components in percentage by mass: 1: 3: pseudomonas aeruginosa, Nitrobacter iso-oxydans, Bacillus circulans and TAD 1.
In the second step, the specific process of culturing is as follows: performing a first stage aeration cycle at 15 deg.C for 3 days with dissolved oxygen of 6mg/L, adding nutrient solution, and performing a second stage aeration cycle for 4 days with dissolved oxygen of 3 mg/L; then adding the filter residue obtained in the first step, and performing a third-stage closed aeration circulation at the temperature of 7 ℃ for 2 days, wherein the dissolved oxygen is 2 mg/L; the nutrient solution is glucose.
Example 3
A method for culturing strains comprises the following steps:
the method comprises the following steps: crushing and filtering soybean residue, taking filtered water, retaining filter residue, adding a porous material into the filtered water, and soaking for 5 hours, wherein the soaked porous material is used as a carrier; the addition amount of the porous material is 30 percent of the mass of the filtered water; the porous material is activated carbon.
Step two: adding clear water into the carrier obtained in the step one, and then adding a biochemical microbial inoculum accounting for 3% of the mass of the clear water for culturing to obtain a strain and a biochemical liquid containing the strain; the biochemical bacteria agent comprises the following components in percentage by mass: 1: 3: pseudomonas aeruginosa, Nitrosomonas, Bacillus circulans, and TAD 1.
In the second step, the specific process of culturing is as follows: performing a first stage aeration cycle at 10 deg.C for 1 day with dissolved oxygen of 3mg/L, adding nutrient solution, and performing a second stage aeration cycle for 3 days with dissolved oxygen of 1 mg/L; then adding the filter residue obtained in the first step, and performing a third-stage closed aeration circulation at the temperature of 5 ℃ for 1 day, wherein the dissolved oxygen is 0.5 mg/L; the nutrient solution is glucose.
Example 4
This example is a modification of example 2, and the specific process of impregnation is ultrasonic impregnation.
Example 5
This example is a modification of example 4, and specifically the porous material is a mixture of activated carbon and vermiculite in a mass ratio of 1: 1.
Example 6
This example is a modification of example 4, and specifically the porous material is a mixture of activated carbon, vermiculite and manganese sand in a mass ratio of 1:1: 1.
Example 7
The embodiment is a change made on the basis of embodiment 4, and specifically, the porous material is a porous material prepared from the following components in a mass ratio of 1:1: 1:1, activated carbon, vermiculite, iron-rich filler and manganese sand.
Comparative example 1
The comparative example is a change made on the basis of example 2, in particular to a culture method of a strain, which comprises the following steps:
adding the carrier into clear water, then adding a biochemical microbial inoculum accounting for 0.5 percent of the mass of the clear water, and culturing to obtain a strain and a biochemical liquid containing the strain; the biochemical bacteria agent comprises the following components in percentage by mass: 1: 3: pseudomonas aeruginosa, nitrobacter iso-oxydans, bacillus circulans and TAD1 of 5; the carrier is active carbon;
the specific process of culture is as follows: performing a first stage aeration cycle at 25 deg.C for 4 days with dissolved oxygen of 10mg/L, adding nutrient solution, and performing a second stage aeration cycle for 5 days with dissolved oxygen of 5 mg/L; then adding the filter residue obtained in the first step, and performing a third-stage closed aeration circulation at the temperature of 10 ℃ for 3 days, wherein the dissolved oxygen is 3 mg/L; the nutrient solution is glucose.
Comparative example 2
A method for culturing strains comprises the following steps:
the method comprises the following steps: crushing and filtering soybean residue, taking filtered water, retaining filter residue, adding a porous material into the filtered water, and soaking for 7 hours, wherein the soaked porous material is used as a carrier; the addition amount of the porous material is 50 percent of the mass of the filtered water; the porous material is activated carbon.
Step two: adding clear water into the carrier obtained in the step one, and then adding a biochemical microbial inoculum accounting for 2% of the mass of the clear water for culturing to obtain a strain and a biochemical liquid containing the strain; the biochemical bacteria agent comprises the following components in percentage by mass: 1: 3: pseudomonas aeruginosa, Nitrobacter iso-oxydans, Bacillus circulans and TAD 1.
In the second step, the specific process of culturing is as follows: performing a first-stage aeration cycle at 15 ℃ for 3 days, wherein the dissolved oxygen is 6mg/L, adding a nutrient solution, and then adding the filter residue obtained in the first step; the nutrient solution is glucose.
And mixing the biochemical liquid containing the strains obtained by the cultivation method of any embodiment with the industrial wastewater according to the ratio of 1: 3, adding the strains cultured in the above examples, adding the strains in an amount of 5% of the mass of the industrial wastewater, aerating for 48 hours, and carrying out industrial wastewater treatment, wherein the index values of the wastewater after the treatment are shown in table 2; the temperature of the industrial wastewater is 10 ℃.
Wherein the industrial wastewater is industrial wastewater of Jinxi sewage treatment plants, and the water quality of the industrial wastewater is shown in Table 1;
table 1 shows the quality of industrial wastewater (unit: mg/L) from Jinxi sewage treatment plant
| Index (I) | COD | TN | TP | Ammonia nitrogen |
| Range of | 350.23 | 104.21 | 3.17 | 109.42 |
Table 2 shows the index test values (unit: mg/L) of the examples and comparative examples after treatment of industrial wastewater at 10 deg.C
| Test specimen | COD | TN | TP | Ammonia nitrogen |
| Example 1 | 50.12 | 11.24 | 0.41 | 6.12 |
| Example 2 | 49.23 | 11.02 | 0.39 | 6.05 |
| Example 3 | 50.21 | 11.36 | 0.38 | 6.11 |
| Example 4 | 48.57 | 10.98 | 0.35 | 6.01 |
| Example 5 | 46.24 | 10.21 | 0.36 | 5.98 |
| Example 6 | 46.13 | 10.15 | 0.34 | 5.75 |
| Example 7 | 40.87 | 9.87 | 0.31 | 5.68 |
| Comparative example 1 | 65.12 | 15.47 | 0.67 | 9.24 |
| Comparative example 2 | 50.16 | 12.54 | 0.58 | 8.14 |
The industrial wastewater treatment of example 7 was carried out at 5 ℃, 10 ℃, 15 ℃ and 30 ℃, and the test values thereof are shown in Table 3;
table 3 shows the index values (unit: mg/L) of wastewater treatment at 5 ℃, 10 ℃ and 15 ℃ and 30 ℃ in example 7
| Test specimen | COD | TN | TP | Ammonia nitrogen |
| 5℃ | 41.54 | 9.97 | 0.32 | 5.75 |
| 10℃ | 40.57 | 9.87 | 0.31 | 5.68 |
| 15℃ | 40.79 | 9.52 | 0.29 | 5.12 |
| 30℃ | 39.87 | 8.12 | 0.28 | 5.01 |
As can be seen from Table 2, the culture methods of examples 1 to 7 yielded the bacterial species and the biochemical liquid containing the bacterial species having higher water treatment effects at low temperatures (10 ℃ C.) than those of comparative examples 1 and 2.
The above results were analyzed for the following reasons:
compared with the comparative example 1, because the soybean dregs are soaked in the porous material in the examples 1 to 7, on one hand, a nitrogen source and a carbon source required in the culture process are adsorbed in the pores of the porous material in advance, which is beneficial to the subsequent nutrition supplement of strains, and on the other hand, the soybean dregs contain rich iron and are adsorbed in the gaps of the porous material, so that the iron can stimulate the strains with low activity at low temperature, and the activity and the quantity of the strains are improved, thereby improving the wastewater treatment effect; in the embodiment 4, the ultrasonic impregnation is adopted, so that the porous material can adsorb the filtered water more uniformly, each pore diameter in the porous material is filled with the filtered water, the nutrition supplement of strains and the subsequent release of iron are facilitated, and the activity of the strains is stimulated; in the embodiment 6, the manganese sand is added, manganese element is released during wastewater treatment, and the manganese element is one of essential trace elements for microbial growth and reproduction, so that the manganese sand is applied to the field of sewage treatment, can stimulate the growth and reproduction of microbes in a treatment system, and has a positive effect on improving the biochemical treatment effect; compared with the comparative example 2, the method mainly adopts the step-by-step stuffy aeration of the strains at different low temperature in the examples 1 to 7, namely, oxygen is gradually supplied at different low temperature to stimulate the strains, so that the strains can adapt to the wastewater environment in time at low temperature, and the water treatment effect is improved. .
As can be seen from Table 3, the difference of the treatment effect of example 7 at 5 deg.C, 10 deg.C, 15 deg.C and 30 deg.C is not very large, which shows that the activity of the strain at 5-15 deg.C is not much different from the activity of the strain at 30 deg.C in this example, which shows that the strain obtained by the cultivation method of the present invention can maintain the activity well at low temperature, mainly because the iron-rich filler is added in example 7, besides the reason of the above-mentioned soybean dregs, on one hand, because the iron element of the iron-rich filler stimulates the microorganism, on the other hand, the iron-rich filler itself also has good water treatment effect, i.e. the iron-rich filler is a cheap filler processed by iron oxide phosphorus and mineral powder through multiple processes, because it has loose and porous sponge structure, it has the advantages of fast iron dissolution rate and large specific surface area, the sponge structure of the iron-rich filler combines with the porous structure of the activated carbon, so it has very excellent physical, thus, a good effect can be obtained in wastewater treatment.
The test index method described above is shown in Table 4.
TABLE 4 Water quality index determination method
The above additional technical features can be freely combined and used in superposition by those skilled in the art without conflict.
The above description is only a preferred embodiment of the present invention, and the technical solutions that achieve the objects of the present invention by basically the same means are all within the protection scope of the present invention.
Claims (10)
1. A method for cultivating strains is characterized in that: the method comprises the following steps:
the method comprises the following steps: crushing and filtering soybean residue, taking filtered water, retaining filter residue, adding a porous material into the filtered water for impregnation, wherein the impregnated porous material is used as a carrier;
step two: and (3) adding clear water into the carrier obtained in the step one, then adding a biochemical microbial inoculum, and culturing to obtain a strain and a biochemical liquid containing the strain.
2. A method of growing a bacterial species according to claim 1, wherein: in the first step, the porous material is one or more of activated carbon, vermiculite and manganese sand.
3. A method of growing a bacterial species according to claim 1, wherein: in the step one, the specific process of dipping is ultrasonic dipping for 5-9 h.
4. A method of growing a bacterial species according to claim 1, wherein: in the second step, the specific process of culturing is as follows: performing first-stage aeration circulation at 10-25 deg.C, adding nutrient solution, performing second-stage aeration circulation, adding the residue, and performing third-stage aeration circulation at 5-10 deg.C.
5. A method of growing a bacterial species according to claim 4, wherein: in the second step, the closed aeration circulation in the first stage is carried out for 1 to 4 days, wherein the dissolved oxygen is 3 to 10 mg/L; in the second stage, aeration circulation is performed for 3-5 days, wherein the dissolved oxygen is 1-5 mg/L; in the third stage, aeration cycle is performed for 1-3 days, wherein the dissolved oxygen is 0.5-3 mg/L.
6. A method of growing a bacterial species according to claim 4, wherein: in the second step, the nutrient solution is glucose.
7. A method of growing a bacterial species according to claim 1, wherein: in the second step, the biochemical bacteria agent comprises denitrifying bacteria, nitrifying bacteria, bacillus and staphylococcus.
8. A method of growing a bacterial species according to claim 1, wherein: in the second step, the addition amount of the biological agent is 0.5-3% of the mass of the clean water.
9. The application of the culture method of the strain in water treatment is characterized in that: mixing the biochemical liquid obtained by the cultivation method according to any one of claims 1 to 8 with industrial wastewater, and then continuing to add the strain for industrial wastewater treatment.
10. The use of a method of growing a bacterial species according to claim 9 in water treatment, wherein: the temperature of the industrial wastewater is 5-15 ℃.
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Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2005224720A (en) * | 2004-02-13 | 2005-08-25 | Techno Network Shikoku Co Ltd | Bacteria reactor and algae reactor for water treatment, and water purification apparatus using them |
| CN102887587A (en) * | 2012-11-05 | 2013-01-23 | 哈尔滨北方环保工程有限公司 | Method for treating sewage by adopting multi-stage type bio-aeration pool |
| CN103877934A (en) * | 2014-03-26 | 2014-06-25 | 西北师范大学 | Method for preparing porous carbon material from bean dregs and application of porous carbon material serving as adsorbent in wastewater treatment |
| CN105600945A (en) * | 2016-01-29 | 2016-05-25 | 南京神克隆科技有限公司 | Method for treating sewage by utilizing compound bacteria |
| CN109897869A (en) * | 2018-08-28 | 2019-06-18 | 福建省农业科学院农业工程技术研究所 | A kind of high sugared waste water culture using smallfruit fig leaf as the biological activated carbon of carbon source carrier |
| CN111039523A (en) * | 2020-01-08 | 2020-04-21 | 常州工学院 | Isolated circulating biological aerated filter, water treatment system and water treatment method |
| CN111187103A (en) * | 2020-03-16 | 2020-05-22 | 山西宁邦生物科技有限公司 | Sludge compost heavy metal passivation microbial inoculum, and production method and application thereof |
-
2020
- 2020-06-04 CN CN202010502085.9A patent/CN111548973A/en active Pending
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2005224720A (en) * | 2004-02-13 | 2005-08-25 | Techno Network Shikoku Co Ltd | Bacteria reactor and algae reactor for water treatment, and water purification apparatus using them |
| CN102887587A (en) * | 2012-11-05 | 2013-01-23 | 哈尔滨北方环保工程有限公司 | Method for treating sewage by adopting multi-stage type bio-aeration pool |
| CN103877934A (en) * | 2014-03-26 | 2014-06-25 | 西北师范大学 | Method for preparing porous carbon material from bean dregs and application of porous carbon material serving as adsorbent in wastewater treatment |
| CN105600945A (en) * | 2016-01-29 | 2016-05-25 | 南京神克隆科技有限公司 | Method for treating sewage by utilizing compound bacteria |
| CN109897869A (en) * | 2018-08-28 | 2019-06-18 | 福建省农业科学院农业工程技术研究所 | A kind of high sugared waste water culture using smallfruit fig leaf as the biological activated carbon of carbon source carrier |
| CN111039523A (en) * | 2020-01-08 | 2020-04-21 | 常州工学院 | Isolated circulating biological aerated filter, water treatment system and water treatment method |
| CN111187103A (en) * | 2020-03-16 | 2020-05-22 | 山西宁邦生物科技有限公司 | Sludge compost heavy metal passivation microbial inoculum, and production method and application thereof |
Non-Patent Citations (4)
| Title |
|---|
| LIPING LOU 等: "Adsorption and degradation in the removal of nonylphenol from water by cells immobilized on biochar", 《CHEMOSPHERE》 * |
| 宫林林: "改性生物活性炭过滤工艺除氮效能及机理研究", 《中国优秀硕士论文全文数据库 工程科技Ⅰ辑》 * |
| 张斌阁 等: "豆制品加工废水生物制氢系统启动与运行优化", 《哈尔滨商业大学学报(自然科学版)》 * |
| 高阳 等: "活性炭为载体的好氧颗粒污泥培养及性能研究", 《水资源与水工程学报》 * |
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