CN100537746C - Have the Dell Ford bacterium of aerobic denitrification capability and the method for processing waste water thereof - Google Patents
Have the Dell Ford bacterium of aerobic denitrification capability and the method for processing waste water thereof Download PDFInfo
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- CN100537746C CN100537746C CNB2006101408723A CN200610140872A CN100537746C CN 100537746 C CN100537746 C CN 100537746C CN B2006101408723 A CNB2006101408723 A CN B2006101408723A CN 200610140872 A CN200610140872 A CN 200610140872A CN 100537746 C CN100537746 C CN 100537746C
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W10/00—Technologies for wastewater treatment
- Y02W10/10—Biological treatment of water, waste water, or sewage
Abstract
The present invention relates to Dell's Ford (Delftia tsuruhatensis) WXZ-9CGMCC No.1797 bacterial strain that a strain has aerobic denitrification capability and be applied to the method that denitrogenation of waste water is handled.This bacterial strain is strong to the tolerance degree of dissolved oxygen, under 10～98% saturated dissolved oxygen conditions, all can be with significant speed reduction nitrate and nitrite, and it is inoculated in the active sludge after the enrichment culture, can realize the aerobic denitrification effect.
Description
Technical field
The present invention relates to a kind of employing Dell's Ford (Delftia tsuruhatensis) WXZ-9 CGMCC No.1797 bacterial strain and under aerobic condition, carry out the method that biological denitrificaion is handled waste water.This bacterial strain is strong to the tolerance degree of dissolved oxygen, under 10～98% saturated dissolved oxygen conditions, can utilize nitrate and nitrite as electron acceptor(EA).Nitrate in the sewage and nitrite can be reduced to gaseous product and make denitrogenation of waste water under aerobic environment.
Background technology
In the multiple denitrogenation method of having used at present, biological denitrificaion remains the main means in the denitrogenation of waste water processing.Traditional wastewater biological denitrificaion treatment process is: the organic nitrogen compound in the sewage at first is converted into ammonia nitrogen in biological process, ammonia nitrogen is translated into nitrate and nitrite by the autotrophy nitrifier in nitrifying process; In denitrification process, by the heterotrophic denitrification bacterium nitrate and nitrite reduction are converted into gaseous product and make denitrogenation of waste water again.Nitrated is the prerequisite of denitrification process, but denitrification process really reaches the purpose that the nitrogenous compound in the waste water removes.Because nitrifier has the intensive aerobic, nitrifying process must be aerobic; And the classical inverse nitrifier under aerobic conditions promptly with oxygen as electron acceptor(EA), carry out aerobic repiration, when having only anaerobic state just with nitrate or nitrite as electron acceptor(EA), obtain the energy of synthetic cell body, so the classical inverse nitrifier only can just can carry out denitrification under anaerobic environment.Usually nitrification and denitrification is carried out in aerobic zone and anoxic or anaerobic district respectively according to the theoretical biological denitrification process that grows up of traditional biological denitrogenation, form classification nitration denitrification technology, thereby must build nitrification tank and anti-nitre pond respectively, so just make stagewise nitration denitrification technology have a lot of weak points, such as owing to build nitrification tank and denitrification pond respectively, make that the build process flow process is long, initial cost is high, need be to denitrification pond supplementary carbon source etc.
Patent of invention " a kind of aerobic biological denitrification method ", ZL 2,003 1 0,121,374 0, this patent is a kind of relating to the nitrogenous compound in the sewage to be carried out the patent of invention of the denitrifying denitrogenation processing of aerobic nitrification simultaneously by the microorganism in the active sludge in same reactor, but this patent does not relate to the kind of microorganism in the active sludge; Many research reports at present both domestic and external confirm that all the aerobic denitrification effect is conventional that exist rather than accidental.In fact, it is very possible isolating aerobic denitrifying bacteria from canal, pond, soil and active sludge.These bacterial classifications of separating belong to Proteobacteria door α, the different Pseudomonas of beta, gamma guiding principle (as Paracoccus, Thiobacillus, Enterobacter, Comamonas, Sphingomonas etc.).In addition, Lukow isolates a strain bacterium TL1 from the nitrated workshop section of percolate, and this bacterium can utilize degraded oxygen and nitrate simultaneously, through identifying that this Pseudomonas is in Zoogloea.Naokia sieve two strain denitrifying bacterias, be respectively Pseudomonas stutzeri TR2 and Pseudomonas sp.K50.H.K.Huang isolates aerobic denitrifying bacteria Citrobacterdiversus from nitration denitrification mud.Domestic Zhang Guangya isolates a strain aerobic denitrifying bacteria HN from soil, through being accredited as Rhodococcus sp., Yu Airong isolates a strain oxytolerant denitrification from the pond, belong to Bacillus through evaluation.The report of relevant Dell Ford bacterium (Delftia) seldom in the present domestic and foreign literature.Doctor Yan Hai of University of Science ﹠ Technology, Beijing screened from the bed mud of Dian Chi, Yunnan 1 strain that more famous is can degrading microcystic toxins (Microcystins, MCs) microorganism pure strain, be accredited as Delftia acidovorans USTB04 through Institute of Microorganism, Academia Sinica, but and find no denitrification.
Summary of the invention
Dell's Ford that this patent relates to (Delftia tsuruhatensis) WXZ-9 CGMCC No.1797 bacterial strain can be grown under the condition that only with nitrate is the nitrogenous source and the energy.This bacterial strain is strong to the tolerance degree of dissolved oxygen, under 10～98% saturated dissolved oxygen conditions, all can denitrification can be carried out under aerobic condition with significant speed reduction nitrate and nitrite, is the newfound aerobic denitrifying bacteria of a strain.This bacterial strain carries out the characteristic of denitrification under aerobic conditions, it can be inoculated in the conventional aerobic nitrification mud, the synchronous nitration and denitrification effect of nitrogenous effluent can be realized, the problem that biological denitrificaion in traditional wastewater treatment need be taked anoxic denitrification, aerobic nitrification staging treating can be solved.The front has introduced that ammonia nitrogen all changes nitrate or nitrite into through nitrifying process in the waste water, so the present invention is applicable to the denitrogenation processing of various nitrogenous effluents.
The objective of the invention is to screen high-efficiency aerobic denitrification bacterial strain, and it is right to carry out bacterial strain order-checking discrimination ratio with spontaneous growth advantage.With the bacterial strain that screening obtains, be inoculated in the waste water that contains different concns nitrate, and, confirm that bacterial strain has adaptability more widely by differential responses condition test (dissolved oxygen, carbon-nitrogen ratio, pH value etc.).The denitrogenation ability of bacterial strain is subjected to having the greatest impact of carbon-nitrogen ratio, and carbon-nitrogen ratio is high more, and the denitrogenation ability of bacterial strain is strong more, though thereby the present invention under carbon-nitrogen ratio 2～10 conditions, experimentize, be 2～30 o'clock in carbon-nitrogen ratio, bacterial strain still has stronger denitrogenation ability.Tolerance degree to dissolved oxygen is strong, and under 10～98% saturated dissolved oxygen conditions, at nitrate concentration 25～250mg/L, carbon-nitrogen ratio is 2～30, all can carry out effective aerobic denitrogenation under the condition of pH5.0～8.0.Specifically, the present invention relates to strain Dell Ford (Delftiatsuruhatensis) WXZ-9 CGMCC No.1797, the grown cell of this bacterial strain, cell suspending liquid bacterium can be the growth of the only nitrogen source and the energy with nitrate, the nitrate that can reduce under aerobic condition is nitrite, and can to continue under aerobic condition the reduction nitrite be gaseous product, make nitrogen compound reduction in the waste water, reach the purpose of denitrogenation.
Dell provided by the invention Ford bacterium (Delftia tsuruhatensis) WXZ-9 CGMCC No.1797, on September 7th, 2006, be preserved in Beijing that " China Committee for Culture Collection of Microorganisms's common micro-organisms " center ", its preserving number are CGMCC No.1797.
This bacterial strain has following feature: (1) colony characteristics: after cultivating 2～3 days on the BTB flat board, the bacterium colony size is 1～2mm, and bacterium colony is circular, and glossiness is fine, blueness, center adularescent projection.(2) cell morphological characteristic: be suitable for about pH neutrality, normal temperature is cultivated down, and cell is shaft-like, Gram-negative.(3) the 16SrRNA gene sequence characteristic of Dell's Ford bacterium (Delftia tsuruhatensis) WXZ-9 CGMCC No.1797: its 16SrRNA has the nucleotide sequence shown in the sequence table, sequence length is 1406bp, and the accession number in Genbank is DQ356901.
With reference to " the content of the outstanding Bacteria Identification handbook of uncle (the 8th edition), according to its morphological specificity and physiological and biochemical property, and, identify that this bacterial strain (CGMCC No.1797) is Dell Ford bacterium (Delftia tsuruhatensis) according to the result for retrieval of its 16SrRNA gene order in Genbank.
This inoculation is selected different condition such as dissolved oxygen in the waste water that contains different concns nitrate, carbon-nitrogen ratio, pH value etc. experimentize under in the different experiments scope, and measure its maximum denitrifying capacity.Experimental result shows, this bacterial strain is in carbon-nitrogen ratio=2～10, the nitrate wastewater concentration of representing with nitrogen element content is at 50～200mg/L, reaction rotating speed 60～160rpm, be equivalent to dissolved oxygen concentration 1.8～7.2mg/L (24～95% saturated dissolved oxygen), in the scope of experiment of pH=6.5～7.5, its nitrate maximum removal rate can reach 159.0mg NO
3 --N/Ld, maximum 4 days nitrate reduction ratios reach 100%, and maximum 4 days nitrogen removal rates reach 76.3%.
Dell provided by the invention Ford bacterium (Delftia tsuruhatensis) WXZ-9 CGMCC No.1797 bacterial strain can be grown in nitrate wastewater, and degraded utilizes nitrate under aerobic condition, and carries out the denitrification denitrogenation effect; Can be to grow in carbon source, the nitrogenous source with the nutritional medium also as beef peptone, nutrient agar medium etc.
Description of drawings
Selected each level of four factors of accompanying drawing 1 Dell Ford bacterium (Delftia tsuruhatensis) CGMCC No.1797-nitrate clearance
The degradation curve of accompanying drawing 2 Dell Ford bacterium (Delftia tsuruhatensis) CGMCC No.1797 nitrate under optimal conditions
Concrete embodiment
In order to understand the present invention better, further specified by following embodiment, but be not limitation of the invention.
Embodiment 1: the measuring method of Dell Ford bacterium (Delftia tsuruhatensis) CGMCC No.1797 bacterial strain aerobic denitrification capability
In order to prevent to bring assorted bacterium in the simulated wastewater into, nitrate simulated wastewater I and nitrate simulated wastewater II be deactivation 20min in high-temperature sterilization pot 0.1MPa all.Get nitrate simulated wastewater I after the 5ml sterilization and pack in the test tube, insert pure Dell Ford bacterium (Delftia tsuruhatensis) the CGMCC No.1797 bacterial strain sample of preserving with the whiteruss envelope.Test tube is placed gas bath constant temperature shaking table, and constant temperature keeps 30 ℃, and shaking speed 160r/min carries out the bacterial strain activation.Activate after 12 hours, the inoculum size with 10% is inoculated into carries out denitrification, nitrogen removal performance mensuration in the 250ml Erlenmeyer flask that 200ml nitrate simulated wastewater II is housed.For guaranteeing the exchange of air and ambient atmos in the Erlenmeyer flask, seal to seal film, similarly on 30 ℃, gas bath constant temperature shaking table, carry out shake-flask culture with 160r/min.For the 160r/min speed test that adds waste water 200ml in the 250ml Erlenmeyer flask, actual measurement reaction solution dissolved oxygen can remain on 6.0～7.2mg/L, is equivalent to 79～95% saturated dissolved oxygen.Under the similarity condition, blank wastewater sample (not meeting the nitrate simulated wastewater II of bacterial strain) is reference test as a comparison.
Except original state, filter wastewater sample 5ml in the extraction Erlenmeyer flask with the pin type strainer that 0.2 μ m blend fiber film is housed every 24h, adopt the nitrate and the nitroso-group nitrogen concentration of waste water in the ion-chromatographic determination Erlenmeyer flask.Analyze the denitrification capability and the nitrogen removal performance of bacterial strain by the clearance that calculates nitrate removal rate and total nitrogen (nitrate and nitrite concentration sum in the waste water).
Nitrate simulated wastewater I (/L): KNO
31g; KH
2PO
41g; FeCl
26H
2O 0.5g; CaCl
27H
2O 0.2g; MgSO
47H
2O 1g; Sodium succinate 8.5g.
Nitrate simulated wastewater II (/L): KNO
30.6g; KH
2PO
41g; MgSO
47H
2O 1g; Sodium succinate 2.4g.
Embodiment 2-10: different experimental conditions, scope are determined the best denitrification condition of Dell Ford bacterium (Delftia tsuruhatensis) CGMCCNo.1797 bacterial strain
The present invention carries out wastewater treatment test altogether 9 times, the aerobic denitrification reaction conditions of preferred Dell Ford bacterium (Delftia tsuruhatensis) WXZ-9CGMCC No.1797 bacterial strain.
Four reaction factors of waste water aerobic denitrification are respectively: dissolved oxygen concentration, rpm realizes by the control different rotating speeds, three levels of rotating speed are respectively 60,100 and 160rpm, and corresponding dissolved oxygen concentration is respectively 1.8～3.5mg/L (24～46% saturated dissolved oxygen), 4.2～5.6mg/L (55～74% saturated dissolved oxygen) and 6～7.2mg/L (79～95% saturated dissolved oxygen); Three levels of nitrate concentration be respectively 50,100 and 200mg/L about; Three levels of carbon-nitrogen ratio are respectively 2,6 and 10; Three levels of pH are respectively 6.5,7.0 and 7.5.
Carry out denitrification capability according to embodiment 1 method and measure, 9 times test-results sees Table 1, and makes accompanying drawing 1.By accompanying drawing 1 as seen, Dell Ford bacterium (Delftia tsuruhatensis) WXZ-9 CGMCC No.1797 is subjected to having the greatest impact of carbon-nitrogen ratio to the removal ability of nitrate, when carbon-nitrogen ratio is 2 left and right sides, it is 35.6% that the nitrate removal rate has only, and when carbon-nitrogen ratio increased to 10 left and right sides, the nitrate removal rate was increased to 86.2%; Dissolved oxygen is also bigger to the influence that Dell Ford bacterium (Delftia tsuruhatensis) WXZ-9 CGMCC No.1797 removes the nitrate ability, when dissolved oxygen 1.8～3.5mg/L (24～46% saturated dissolved oxygen), the nitrate removal rate is 84.4%, when dissolved oxygen 6～7.2mg/L (79～95% saturated dissolved oxygen), the nitrate removal rate reduces to 43.6%; Nitrate concentration in the waste water influences the influence that is slightly less than dissolved oxygen to the ability that Dell Ford bacterium (Delftia tsuruhatensis) WXZ-9CGMCC No.1797 removes nitrate, when in the waste water during nitrate concentration 200mg/L left and right sides, Dell Ford bacterium (Delftia tsuruhatensis) WXZ-9 CGMCC No.1797 reaches 57.9% to the clearance of nitrate; Minimum relatively about the pH neutral range to the ability influence of Dell Ford bacterium (Delftia tsuruhatensis) WXZ-9 CGMCCNo.1797 removal nitrate.
In scope of experiment, embodiment 8 C/N=10, NO
3 --N=230.5mg/L, 60r/min, pH=7.0, Dell's Ford bacterium (Delftia tsuruhatensis) CGMCC No.1797 can be degraded to 71.4mg/L from 230.5mg/L with nitrate concentration in one day under this condition, and maximum four days average nitrates are removed speed and can be reached 150.9mg NO
3 --N/Ld (seeing accompanying drawing 2), maximum 4 days nitrate clearances reach 100%, and maximum 4 days nitrogen removal rates reach 76.3%.
Waste water aerobic denitrification test condition and the nitric efficiency of table 1 embodiment 2-10
| The embodiment numbering | Rotating speed/rpm | C/N | No 3 --N /(mg/L) | pH | Four days average NO 3 --N removes speed % | Four days average NO X --N removes speed % |
| |
160 | 2 | 191.7 | 6.5 | 20.6 | 17.9 |
| |
100 | 2 | 106.1 | 7.0 | 33.0 | 23.9 |
| |
60 | 2 | 56.1 | 7.5 | 53.3 | 30.6 |
| Embodiment 5 | 100 | 6 | 212.3 | 7.5 | 53.0 | 25.8 |
| Embodiment 6 | 60 | 6 | 113.8 | 6.5 | 100 | 44.9 |
| Embodiment 7 | 160 | 6 | 61.3 | 7.0 | 51.7 | 48.8 |
| Embodiment 8 | 60 | 10 | 230.5 | 7.0 | 100 | 76.3 |
| Embodiment 9 | 160 | 10 | 126 | 7.5 | 58.6 | 54.6 |
| Embodiment 10 | 100 | 10 | 63.8 | 6.5 | 100 | 60.8 |
Embodiment 11: the pcr amplification and the sequencing of the 16SrRNA gene of Dell Ford bacterium (Delftia tsuruhatensis) CGMCC No.1797
CGMCC No.1797 is inoculated in the 100 μ L distilled waters with Dell Ford bacterium (Delftia tsuruhatensis), and boiling water bath boils 5min, carries out colony PCR amplification.The primer that is used for 16SrRNAPCR reaction is a pair of universal primer, and promptly forward primer is that f27 (5 '-AGAGTTGATCCTGGCTCAG-3 ') and reverse primer are r1492 (5 '-GGTTACCTTGTTACGACTT-3 ').Primer is synthetic by Beijing China big-and-middle living development in science and technology company limited.PCR reaction system (50 μ L): 10 * Buffer, 5 μ L, dNTPs 4 μ L, each 1 μ L of primer f27 and r1492, dual distilled water 39 μ L add dna profiling 0.5 μ L, Taq enzyme 0.5 μ L behind the centrifugal mixing.The PCR response procedures comprises: (1) 94 ℃ of pre-sex change 5min; (2) 94 ℃ of sex change 1min; (3) 50 ℃ of annealing 1min; (4) 72 ℃ are extended 2min, carry out 25 circulations; At last once more 72 ℃ extend 10min.Agarose gel electrophoresis (1 * TAE electrophoretic buffer, 1% gel) is analyzed PCR result, transfers to Beijing China big-and-middle living development in science and technology company limited and carries out the 16SrRNA order-checking.16SrRNA gene order length is 1406bp, and the accession number in Genbank is DQ356901, and the nucleotide sequence of its 16SrRNA is shown in sequence table.
＜110〉Beijing Technology and Business University
＜120〉have the Dell Ford bacterium of aerobic denitrification capability and the method for processing waste water thereof
<140>2006101408723
<141>2006-10-13
<160>1
<210>1
<211>1406
<212>DNA
＜213〉Dell's Ford bacterium (Delftia tsuruhatensis)
<400>1
catgcagtcg aacggtaaca ggtcttcgga cgctgacgag tggcgaacgg gtgagtaata 60
catcggaacg tgcccagtcg tgggggataa ctactcgaaa gagtagctaa taccgcatac 120
gatctgagga tgaaagcggg ggaccttcgg gcctcgcgcg attggagcgg ccgatggcag 180
attaggtagt tggtgggata aaagcttacc aagccgacga tctgtagctg gtctgagagg 240
acgaccagcc acactgggac tgagacacgg cccagactcc tacgggaggc agcagtgggg 300
aattttggac aatgggcgaa agcctgatcc agcaatgccg cgtgcaggat gaaggccttc 360
gggttgtaaa ctgcttttgt acggaacgaa aaagctcctt ctaatacagg gggcccatga 420
cggtaccgta agaataagca ccggctaact acgtgccagc agccgcggta atacgtaggg 480
tgcgagcgtt aatcggaatt actgggcgta aagcgtgcgc aggcggttat gtaagacaga 540
tgtgaaatcc ccgggctcaa cctgggaact gcatttgtga ctgcatggct agagtacggt 600
agagggggat ggaattccgc gtgtagcagt gaaatgcgta gatatgcgga ggaacaccga 660
tggcgaaggc aatcccctgg acctgtactg acgctcatgc acgaaagcgt ggggagcaaa 720
caggattaga taccctggta gtccacgccc taaacgatgt caactggttg ttgggaatta 780
gttttctcag taacgaagct aacgcgtgaa gttgaccgcc tggggagtac ggccgcaagg 840
ttgaaactca aaggaattga cggggacccg cacaagcggt ggatgatgtg gtttaattcg 900
atgcaacgcg aaaaacctta cccacctttg acatggcagg aagtttccag agatggattc 960
gtgctcgaaa gagaacctgc acacaggtgc tgcatggctg tcgtcagctc gtgtcgtgag 1020
atgttgggtt aagtcccgca acgagcgcaa cccttgtcat tagttgctac attcagttga 1080
gcactctaat gagactgccg gtgacaaacc ggaggaaggt ggggatgacg tcaagt cctc 1140
atggccctta taggtggggc tacacacgtc atacaatggc tggtacagag ggttgccaac 1200
ccgcgagggg gagctaatcc cataaaacca gtcgtagtcc ggatcgcagt ctgcaactcg 1260
actgcgtgaa gtcggaatcg ctagtaatcg cggatcagca tgccgcggtg aatacgttcc 1320
cgggtcttgt acacaccgcc cgtcacacca tgggagcggg tctcgccaga agtaggtagc 1380
ctaaccgcaa ggagggcgct accacg 1406
Claims (7)
1. a strain has Dell's Ford (Delftia tsuruhatensis) WXZ-9CGMCC No.1797 that can carry out the denitrification performance under aerobic condition, it is characterized in that its deposit number is CGMCC No.1797.
2. the Ford WXZ-9 CGMCC No.1797 of Dell according to claim 1 is characterized in that 16SrRNA is the nucleotide sequence shown in sequence table, and sequence length is 1406bp, and the accession number in Genbank is DQ356901.
3. the Ford WXZ-9CGMCC No.1797 of Dell bacterial strain according to claim 1, it is characterized in that: colony characteristics: after cultivating 2～3 days on the BTB flat board, the bacterium colony size is 1～2mm, and bacterium colony is circular, and glossiness is fine, blueness, center adularescent projection; Cell morphological characteristic: be suitable for pH neutrality, normal temperature is cultivated down, and cell is shaft-like, Gram-negative.
4. the Ford WXZ-9CGMCC No.1797 of Dell according to claim 1, grown cell, the cell suspending liquid that it is characterized in that this bacterial strain can be the growth of the only nitrogen source and the energy with nitrate, the nitrate that can reduce under aerobic condition is nitrite, and can to continue under aerobic condition the reduction nitrite be gaseous product, make nitrogenous compound reduction in the waste water, reach the purpose of denitrogenation.
5. the Ford WXZ-9CGMCC No.1797 of Dell according to claim 4, its feature comprises: grow in nitrate wastewater, utilize nitrate to carry out the denitrification denitrogenation effect as electron acceptor(EA) under aerobic condition; Or in beef peptone, nutrient agar medium, grow.
6. the Ford WXZ-9CGMCC No.1797 of Dell according to claim 1, it is characterized in that this bacterial strain has adaptability more widely, tolerance degree to dissolved oxygen is strong, under 10～98% saturated dissolved oxygen conditions, at nitrate concentration 25～250mg/L, carbon-nitrogen ratio is 2～30, all can carry out effective aerobic denitrogenation under the condition of pH5.0～8.0.
7. according to claim 1 or the 5 or 6 described Ford WXZ-9CGMCC No.1797 of Dell, it is characterized in that under aerobic conditions, having the denitrification characteristic of extensive adaptation, it is inoculated in the conventional aerobic nitrification mud, can realizes the synchronous nitration and denitrification effect of nitrogenous effluent.
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| CN101993838A (en) * | 2010-07-08 | 2011-03-30 | 浙江工业大学 | Delftia tsuruhatensis strain H1 with chloroaniline degradation capacity and application thereof |
| CN102674618B (en) * | 2011-03-10 | 2014-02-12 | 北京科技大学 | High-efficient treatment method for biologically-enhanced coking waste water for biological membrane |
| CN102432100B (en) * | 2011-10-27 | 2013-08-14 | 北京工商大学 | Method for efficiently degrading morpholine waste water |
| CN105039222B (en) * | 2015-08-14 | 2019-02-01 | 浙江工业大学 | Dell Ford bacterium LW26 and its application in degradation chlorobenzene |
| CN106635918B (en) * | 2017-01-16 | 2020-04-17 | 轻工业环境保护研究所 | Delftia sp and application thereof in excess sludge reduction and dehydration |
| CN108342339B (en) * | 2018-01-16 | 2020-04-03 | 浙江双良商达环保有限公司 | Klebsiella strain and application thereof in river sewage and rural ammonia nitrogen-containing domestic sewage |
| CN108949611B (en) * | 2018-06-08 | 2021-07-13 | 暨南大学 | Delftit DNF-02 and application thereof in wastewater denitrification |
| TR201901053A2 (en) | 2019-01-23 | 2020-08-21 | Petkim Petrokimya Holding Anonim Sirketi | |
| CN111088200B (en) * | 2020-02-25 | 2022-10-04 | 山东大学 | Heterotrophic nitrification aerobic denitrifying bacterium and application thereof in pig-raising wastewater treatment |
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Non-Patent Citations (4)
| Title |
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| . GenBank登录号DQ356901. 2006 |
| . GenBank登录号DQ356901. 2006 * |
| 废水脱氮系统中好氧反硝化菌的筛选与鉴定. 项慕飞等.食品科技，第2006年卷第7期. 2006 |
| 废水脱氮系统中好氧反硝化菌的筛选与鉴定. 项慕飞等.食品科技，第2006年卷第7期. 2006 * |
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| CN101914480A (en) * | 2010-07-09 | 2010-12-15 | 浙江大学 | Non-pathogenic engineering bacteria with capability of removing nitrites and construction method thereof |
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