CN111543399A - Simple breeding method of Amblyseius barkeri - Google Patents
Simple breeding method of Amblyseius barkeri Download PDFInfo
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- CN111543399A CN111543399A CN202010590527.XA CN202010590527A CN111543399A CN 111543399 A CN111543399 A CN 111543399A CN 202010590527 A CN202010590527 A CN 202010590527A CN 111543399 A CN111543399 A CN 111543399A
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- wheat bran
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- amblyseius barkeri
- tyrophagus putrescentiae
- sterilization
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- 241001206567 Neoseiulus barkeri Species 0.000 title claims abstract description 50
- 238000009395 breeding Methods 0.000 title claims abstract description 14
- 235000015099 wheat brans Nutrition 0.000 claims abstract description 94
- 241000611866 Tyrophagus putrescentiae Species 0.000 claims abstract description 47
- 238000004659 sterilization and disinfection Methods 0.000 claims abstract description 42
- 230000001954 sterilising effect Effects 0.000 claims abstract description 38
- 241000238876 Acari Species 0.000 claims abstract description 32
- 238000000034 method Methods 0.000 claims abstract description 22
- 238000004806 packaging method and process Methods 0.000 claims abstract description 11
- 238000007789 sealing Methods 0.000 claims abstract description 11
- 238000010025 steaming Methods 0.000 claims abstract description 11
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 9
- 239000012153 distilled water Substances 0.000 claims abstract description 6
- 239000002184 metal Substances 0.000 claims abstract description 6
- 238000012258 culturing Methods 0.000 claims description 20
- 241000894007 species Species 0.000 claims description 15
- 239000000463 material Substances 0.000 claims description 12
- 238000001514 detection method Methods 0.000 claims description 9
- 239000000203 mixture Substances 0.000 claims description 5
- 238000002203 pretreatment Methods 0.000 claims description 5
- 241000255777 Lepidoptera Species 0.000 claims description 2
- 230000000384 rearing effect Effects 0.000 claims 5
- 235000013312 flour Nutrition 0.000 abstract description 6
- 238000011081 inoculation Methods 0.000 abstract description 3
- 238000011031 large-scale manufacturing process Methods 0.000 abstract description 3
- 239000000575 pesticide Substances 0.000 description 6
- 241000607479 Yersinia pestis Species 0.000 description 5
- 239000000126 substance Substances 0.000 description 5
- 230000001488 breeding effect Effects 0.000 description 4
- 241000488583 Panonychus ulmi Species 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 238000004519 manufacturing process Methods 0.000 description 3
- 241000254124 Aleyrodidae Species 0.000 description 2
- 241000207199 Citrus Species 0.000 description 2
- 235000013339 cereals Nutrition 0.000 description 2
- 235000020971 citrus fruits Nutrition 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
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- 238000004321 preservation Methods 0.000 description 2
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- 235000009467 Carica papaya Nutrition 0.000 description 1
- 241000219172 Caricaceae Species 0.000 description 1
- 235000014036 Castanea Nutrition 0.000 description 1
- 241001070941 Castanea Species 0.000 description 1
- 244000241257 Cucumis melo Species 0.000 description 1
- 235000015510 Cucumis melo subsp melo Nutrition 0.000 description 1
- 206010059866 Drug resistance Diseases 0.000 description 1
- 240000009088 Fragaria x ananassa Species 0.000 description 1
- 244000070406 Malus silvestris Species 0.000 description 1
- 244000046052 Phaseolus vulgaris Species 0.000 description 1
- 235000010627 Phaseolus vulgaris Nutrition 0.000 description 1
- 241000758706 Piperaceae Species 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
- A23K10/37—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/90—Feeding-stuffs specially adapted for particular animals for insects, e.g. bees or silkworms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L3/00—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs
- A23L3/26—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by irradiation without heating
- A23L3/28—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by irradiation without heating with ultraviolet light
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
Abstract
The invention discloses a simple breeding method of Amblyseius barkeri, which comprises the following steps of wheat bran pretreatment: packaging testa Tritici with large-scale sealed bag by wet heat sterilization, and steaming at 105 deg.C for 45min-1h in a steaming cabinet; performing secondary sterilization treatment on the treated wheat bran by a dry heat sterilization method, transferring the wheat bran into an infrared high-temperature sterilization cabinet, pouring the wheat bran subjected to damp heat sterilization into an inlet of a baking device, conveying the wheat bran by a metal conveyor belt, and mixing the wheat bran and distilled water according to a ratio of 20:1 for later use after sterilization treatment; putting into a culture cup filled with sterilized and humidified 10g of wheat bran, picking Tyrophagus putrescentiae into the culture cup under a viewing mirror, adding Tyrophagus putrescentiae (seed), and sealing the mouth of the container with a breathable film. By utilizing the rapid propagation speed of the Tyrophagus putrescentiae, sufficient feed can be provided for the cultivation of the Amblyseius barkeri, so that two procedures of independent cultivation of the flour mites and inoculation of the flour mites during the propagation of predatory mites are reduced in large-scale production.
Description
Technical Field
The invention belongs to the technical field of agriculture, and particularly relates to a simple breeding method of Amblyseius barkeri.
Background
In China, a plurality of agricultural and forest crops are suffered from rampant damascena hazards of pest mites (including red spider, ticks, thrips and tarsal mites) for a long time; in citrus producing areas in China, red spiders and rust ticks are the most main pests; the number of times of chemical pesticide control is adopted every year, the number of times is more than 20, the number of times accounts for about 60-70% of the total annual pesticide dosage, and the yield and the quality of the citrus are seriously influenced; meanwhile, harmful mites such as red spiders are main pests, and fruits such as apples, pears, peaches, chestnuts, jujube guavas, custards, papayas, strawberries and the like are also used; vegetables such as melons, eggplants, beans, peppers, etc.; the agricultural pest mites are prevented and treated by chemical pesticides in various places of China for a long time, so that the pest mites generate drug resistance and vicious circle of cross rising with the drug use times and dosage; directly aggravate the pollution and harm of chemical pesticide to soil, water and ecology, and obviously increase the chemical pesticide residue of agricultural products; the quality of agricultural products and market competitiveness are seriously influenced, and the health of consumers is hidden; at present, chemical pesticide pollution becomes a public nuisance in China and becomes an ecological and social problem concerned by the whole society.
In recent years, relevant departments in China are going to reduce pesticide pollution, improve the safety quality of agricultural products, and come out policy measures one after another, so that biological control and green control technologies are required to be popularized vigorously; wherein, the method for controlling harmful mites by predatory mites which are natural enemies of mite organisms is a main core technology; at present, it is known that in the domestic process of artificial incubation and cultivation of predatory mites, aleyrodids mother seeds, predatory mite mother seeds and fresh wheat bran are required to be added, so that the probability of infecting bacteria and miscellaneous mites is increased, meanwhile, the production and cultivation period is long, and large-scale rapid production cannot be realized; moreover, two workshops of powder mites and predatory mites need to be arranged, a large amount of labor force is needed, and the production cost is increased; can not meet the market demand of large-scale popularization and application.
Disclosure of Invention
The invention aims to provide a simple breeding method of Amblyseius barkeri, which solves the problems in the background technology.
In order to achieve the purpose, the invention provides the following technical scheme: the simple breeding method of Amblyseius barkeri comprises the following specific culture steps:
the method comprises the following steps: pre-treatment of wheat bran: packaging testa Tritici with large-scale sealed bag by wet heat sterilization, and steaming at 105 deg.C for 45min-1h in a steaming cabinet;
step two: performing secondary sterilization treatment on the wheat bran treated in the step one by a dry heat sterilization method, transferring the wheat bran treated in the step one to an infrared high-temperature sterilization cabinet, pouring the wheat bran subjected to damp heat sterilization into an inlet of baking equipment, conveying the wheat bran by a metal conveyor belt, and mixing the wheat bran and distilled water according to a ratio of 20:1 for later use after the wheat bran is subjected to the sterilization treatment;
step three: purifying and cultivating Tyrophagus putrescentiae: putting into a culture cup filled with 10g of wheat bran sterilized and humidified in the first step and the second step, picking the Tyrophagus putrescentiae into the culture cup under a viewing mirror, adding the Tyrophagus putrescentiae (seed), and sealing the cup mouth of the container by using a breathable film;
step four: culturing the tyrophagus putrescentiae (seed) added in the step three in a container for 10-30 days, and keeping the tyrophagus putrescentiae (seed) in the culture material for later use when the number of the tyrophagus putrescentiae in each gram reaches 2000;
step five: preparing a mother species of predatory mites: putting 500 amblyseius barkeri mother seeds and 5000 butterflies of feed into a culture cup filled with 10g of wheat bran sterilized and humidified in the first step and the second step, sealing the cup mouth by using a breathable film, and culturing for 7-10 days;
step six: when the number of the amblyseius barkeri and the tyrophagus putrescentiae in each gram of wheat bran in the culture cup is detected to reach 250 and 2500 respectively in the step five, the culture cup can be used as a mother seed for propagation expansion of the culture basin;
step seven: taking the wheat bran sterilized and humidified in the first step, the second step and the mother species of the amblyseius barkeri cultivated in the fifth step, wherein the ratio of the wheat bran to the mother species of the amblyseius barkeri cultivated in the first step to the wheat bran sterilized and humidified in the second step is 1: 2, uniformly mixing the materials in proportion, and adding the mixture into a feeding pot for expanding and large-scale propagation; culturing for 5-7 days;
step eight: and detecting the number of the amblyseius barkeri in each gram of the wheat bran in the seventh step, wherein the number of the amblyseius barkeri in each gram of the wheat bran reaches 250, and packaging for use or further propagation.
Furthermore, the thickness of wheat bran on the conveyor belt in the second step is below 1cm, the temperature in the cabinet is 160 ℃, the time from the cabinet entry to the cabinet exit is about 10min, and the outlet is connected with a plastic barrel sterilized by ultraviolet rays.
Further, the culture temperature in the fourth step was 28 ℃ and the Relative Humidity (RH) was adjusted to 90%.
Further, the culture temperature in the fifth step was 28 ℃ and RH (relative humidity) 90%.
Further, the culturing of the seventh step is carried out under the conditions of a temperature of 28 ℃ and an RH (relative humidity) of 90%.
Further, the number detection of the sixth step and the eighth step is carried out by observing and detecting with a viewing mirror, and the A4 paper after being sterilized by ultraviolet rays is used as a base surface for placing the mites.
Compared with the prior art, the invention has the beneficial effects that:
1. the method combining moist heat and dry heat can effectively improve the sterilization and deinsectization effects of the wheat bran, reduce the water content in the wheat bran and facilitate the preservation.
2. Purifying a Tyrophagus putrescentiae from the aleyrodidae as an artificial feed (mite) of the Amblyseius barkeri to carry out industrial mass breeding culture; leftover wheat bran of grain processing is selected as a culture material for breeding the Tyrophagus putrescentiae.
3. By utilizing the rapid propagation speed of the Tyrophagus putrescentiae, sufficient feed can be provided for the cultivation of the Amblyseius barkeri, so that two procedures of independent cultivation of the flour mites and inoculation of the flour mites during the propagation of predatory mites are reduced in large-scale production.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example 1
The simple breeding method of Amblyseius barkeri comprises the following specific culture steps:
the method comprises the following steps: pre-treatment of wheat bran: packaging testa Tritici with large-scale sealed bag by wet heat sterilization, and steaming at 105 deg.C for 45min in a steaming cabinet;
step two: performing secondary sterilization treatment on the wheat bran treated in the step one by a dry heat sterilization method, transferring the wheat bran treated in the step one to an infrared high-temperature sterilization cabinet, pouring the wheat bran subjected to damp heat sterilization into an inlet of baking equipment, conveying the wheat bran by a metal conveyor belt, and mixing the wheat bran and distilled water according to a ratio of 20:1 for later use after the wheat bran is subjected to the sterilization treatment;
step three: purifying and cultivating Tyrophagus putrescentiae: putting into a culture cup filled with 10g of wheat bran sterilized and humidified in the first step and the second step, picking the Tyrophagus putrescentiae into the culture cup under a viewing mirror, adding the Tyrophagus putrescentiae (seed), and sealing the cup mouth of the container by using a breathable film;
step four: culturing the tyrophagus putrescentiae (seed) added in the step three in a container for 10 days, and reserving the tyrophagus putrescentiae when the number of the tyrophagus putrescentiae in the culture material reaches 2000 per gram;
step five: preparing a mother species of predatory mites: putting 500 amblyseius barkeri mother seeds and 5000 feed tyrophagus putrescentiae into a culture cup filled with 10g of wheat bran sterilized and humidified in the first step and the second step, sealing the cup mouth by using a breathable film, and culturing for 7 days;
step six: when the number of the amblyseius barkeri and the tyrophagus putrescentiae in each gram of wheat bran in the culture cup is detected to reach 250 and 2500 respectively in the step five, the culture cup can be used as a mother seed for propagation expansion of the culture basin;
step seven: taking the wheat bran sterilized and humidified in the first step, the second step and the mother species of the amblyseius barkeri cultivated in the fifth step, wherein the ratio of the wheat bran to the mother species of the amblyseius barkeri cultivated in the first step to the wheat bran sterilized and humidified in the second step is 1: 2, uniformly mixing the materials in proportion, and adding the mixture into a feeding pot for expanding and large-scale propagation; and culturing for 5 days;
step eight: and detecting the number of the amblyseius barkeri in each gram of the wheat bran in the seventh step, wherein the number of the amblyseius barkeri in each gram of the wheat bran reaches 250, and packaging for use or further propagation.
Wherein, the thickness of the wheat bran on the conveyor belt in the second step is below 1cm, the temperature in the cabinet is 160 ℃, the time from the cabinet entry to the cabinet exit is about 10min, and the outlet is connected with a plastic barrel sterilized by ultraviolet rays.
Wherein the culture temperature in the fourth step is 28 ℃ and the Relative Humidity (RH) is adjusted to 90%.
Wherein the culture temperature in the fifth step is 28 ℃ and the RH (relative humidity) is 90%.
Wherein the culturing of the seventh step is performed at a temperature of 28 ℃ and an RH (relative humidity) of 90%.
And the quantity detection of the sixth step and the eighth step adopts a viewing mirror for observation and detection, and the A4 paper after ultraviolet sterilization and disinfection is used as a base surface for placing mites.
Example 2
The simple breeding method of Amblyseius barkeri comprises the following specific culture steps:
the method comprises the following steps: pre-treatment of wheat bran: packaging testa Tritici with large-scale sealed bag by wet heat sterilization, and steaming at 105 deg.C for 55min in a steaming cabinet;
step two: performing secondary sterilization treatment on the wheat bran treated in the step one by a dry heat sterilization method, transferring the wheat bran treated in the step one to an infrared high-temperature sterilization cabinet, pouring the wheat bran subjected to damp heat sterilization into an inlet of baking equipment, conveying the wheat bran by a metal conveyor belt, and mixing the wheat bran and distilled water according to a ratio of 20:1 for later use after the wheat bran is subjected to the sterilization treatment;
step three: purifying and cultivating Tyrophagus putrescentiae: putting into a culture cup filled with 10g of wheat bran sterilized and humidified in the first step and the second step, picking the Tyrophagus putrescentiae into the culture cup under a viewing mirror, adding the Tyrophagus putrescentiae (seed), and sealing the cup mouth of the container by using a breathable film;
step four: culturing the tyrophagus putrescentiae (seed) added in the step three in a container for 20 days, and reserving the tyrophagus putrescentiae when the number of the tyrophagus putrescentiae in the culture material reaches 2000 per gram;
step five: preparing a mother species of predatory mites: putting 500 amblyseius barkeri mother seeds and 5000 feed tyrophagus putrescentiae into a culture cup filled with 10g of wheat bran sterilized and humidified in the first step and the second step, sealing the cup mouth by using a breathable film, and culturing for 8 days;
step six: when the number of the amblyseius barkeri and the tyrophagus putrescentiae in each gram of wheat bran in the culture cup is detected to reach 250 and 2500 respectively in the step five, the culture cup can be used as a mother seed for propagation expansion of the culture basin;
step seven: taking the wheat bran sterilized and humidified in the first step, the second step and the mother species of the amblyseius barkeri cultivated in the fifth step, wherein the ratio of the wheat bran to the mother species of the amblyseius barkeri cultivated in the first step to the wheat bran sterilized and humidified in the second step is 1: 2, uniformly mixing the materials in proportion, and adding the mixture into a feeding pot for expanding and large-scale propagation; and culturing for 6 days;
step eight: and detecting the number of the amblyseius barkeri in each gram of the wheat bran in the seventh step, wherein the number of the amblyseius barkeri in each gram of the wheat bran reaches 250, and packaging for use or further propagation.
Wherein, the thickness of the wheat bran on the conveyor belt in the second step is below 1cm, the temperature in the cabinet is 160 ℃, the time from the cabinet entry to the cabinet exit is about 10min, and the outlet is connected with a plastic barrel sterilized by ultraviolet rays.
Wherein the culture temperature in the fourth step is 28 ℃ and the Relative Humidity (RH) is adjusted to 90%.
Wherein the culture temperature in the fifth step is 28 ℃ and the RH (relative humidity) is 90%.
Wherein the culturing of the seventh step is performed at a temperature of 28 ℃ and an RH (relative humidity) of 90%.
And the quantity detection of the sixth step and the eighth step adopts a viewing mirror for observation and detection, and the A4 paper after ultraviolet sterilization and disinfection is used as a base surface for placing mites.
Example 3
The simple breeding method of Amblyseius barkeri comprises the following specific culture steps:
the method comprises the following steps: pre-treatment of wheat bran: packaging testa Tritici with large-scale sealed bag by wet heat sterilization, and steaming in a steam cabinet at 105 deg.C for 1 hr;
step two: performing secondary sterilization treatment on the wheat bran treated in the step one by a dry heat sterilization method, transferring the wheat bran treated in the step one to an infrared high-temperature sterilization cabinet, pouring the wheat bran subjected to damp heat sterilization into an inlet of baking equipment, conveying the wheat bran by a metal conveyor belt, and mixing the wheat bran and distilled water according to a ratio of 20:1 for later use after the wheat bran is subjected to the sterilization treatment;
step three: purifying and cultivating Tyrophagus putrescentiae: putting into a culture cup filled with 10g of wheat bran sterilized and humidified in the first step and the second step, picking the Tyrophagus putrescentiae into the culture cup under a viewing mirror, adding the Tyrophagus putrescentiae (seed), and sealing the cup mouth of the container by using a breathable film;
step four: culturing the tyrophagus putrescentiae (seed) added in the step three in a container for 30 days, and reserving the tyrophagus putrescentiae when the number of the tyrophagus putrescentiae in the culture material reaches 2000 per gram;
step five: preparing a mother species of predatory mites: putting 500 amblyseius barkeri mother seeds and 5000 feed tyrophagus putrescentiae into a culture cup filled with 10g of wheat bran sterilized and humidified in the first step and the second step, sealing the cup mouth by using a breathable film, and culturing for 10 days;
step six: when the number of the amblyseius barkeri and the tyrophagus putrescentiae in each gram of wheat bran in the culture cup is detected to reach 250 and 2500 respectively in the step five, the culture cup can be used as a mother seed for propagation expansion of the culture basin;
step seven: taking the wheat bran sterilized and humidified in the first step, the second step and the mother species of the amblyseius barkeri cultivated in the fifth step, wherein the ratio of the wheat bran to the mother species of the amblyseius barkeri cultivated in the first step to the wheat bran sterilized and humidified in the second step is 1: 2, uniformly mixing the materials in proportion, and adding the mixture into a feeding pot for expanding and large-scale propagation; culturing for 5-7 days;
step eight: and detecting the number of the amblyseius barkeri in each gram of the wheat bran in the seventh step, wherein the number of the amblyseius barkeri in each gram of the wheat bran reaches 250, and packaging for use or further propagation.
Wherein, the thickness of the wheat bran on the conveyor belt in the second step is below 1cm, the temperature in the cabinet is 160 ℃, the time from the cabinet entry to the cabinet exit is about 10min, and the outlet is connected with a plastic barrel sterilized by ultraviolet rays.
Wherein the culture temperature in the fourth step is 28 ℃ and the Relative Humidity (RH) is adjusted to 90%.
Wherein the culture temperature in the fifth step is 28 ℃ and the RH (relative humidity) is 90%.
Wherein the culturing of the seventh step is performed at a temperature of 28 ℃ and an RH (relative humidity) of 90%.
And the quantity detection of the sixth step and the eighth step adopts a viewing mirror for observation and detection, and the A4 paper after ultraviolet sterilization and disinfection is used as a base surface for placing mites.
The working principle of the invention is as follows: the method combining moist heat and dry heat can effectively improve the sterilization and deinsectization effects of the wheat bran, reduce the water content in the wheat bran and facilitate the preservation; purifying a Tyrophagus putrescentiae from the aleyrodidae as an artificial feed (mite) of the Amblyseius barkeri to carry out industrial mass breeding culture; selecting leftovers wheat bran of grain processing as a culture material for breeding of Tyrophagus putrescentiae; by utilizing the rapid propagation speed of the Tyrophagus putrescentiae, sufficient feed can be provided for the cultivation of the Amblyseius barkeri, so that two procedures of independent cultivation of the flour mites and inoculation of the flour mites during the propagation of predatory mites are reduced in large-scale production.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (6)
1. The simple breeding method of Amblyseius barkeri is characterized by comprising the following specific culture steps:
the method comprises the following steps: pre-treatment of wheat bran: packaging testa Tritici with large-scale sealed bag by wet heat sterilization, and steaming at 105 deg.C for 45min-1h in a steaming cabinet;
step two: performing secondary sterilization treatment on the wheat bran treated in the step one by a dry heat sterilization method, transferring the wheat bran treated in the step one to an infrared high-temperature sterilization cabinet, pouring the wheat bran subjected to damp heat sterilization into an inlet of baking equipment, conveying the wheat bran by a metal conveyor belt, and mixing the wheat bran and distilled water according to a ratio of 20:1 for later use after the wheat bran is subjected to the sterilization treatment;
step three: purifying and cultivating Tyrophagus putrescentiae: putting into a culture cup filled with 10g of wheat bran sterilized and humidified in the first step and the second step, picking the Tyrophagus putrescentiae into the culture cup under a viewing mirror, adding the Tyrophagus putrescentiae (seed), and sealing the cup mouth of the container by using a breathable film;
step four: culturing the tyrophagus putrescentiae (seed) added in the step three in a container for 10-30 days, and keeping the tyrophagus putrescentiae (seed) in the culture material for later use when the number of the tyrophagus putrescentiae in each gram reaches 2000;
step five: preparing a mother species of predatory mites: putting 500 amblyseius barkeri mother seeds and 5000 butterflies of feed into a culture cup filled with 10g of wheat bran sterilized and humidified in the first step and the second step, sealing the cup mouth by using a breathable film, and culturing for 7-10 days;
step six: when the number of the amblyseius barkeri and the tyrophagus putrescentiae in each gram of wheat bran in the culture cup is detected to reach 250 and 2500 respectively in the step five, the culture cup can be used as a mother seed for propagation expansion of the culture basin;
step seven: taking the wheat bran sterilized and humidified in the first step, the second step and the mother species of the amblyseius barkeri cultivated in the fifth step, wherein the ratio of the wheat bran to the mother species of the amblyseius barkeri cultivated in the first step to the wheat bran sterilized and humidified in the second step is 1: 2, uniformly mixing the materials in proportion, and adding the mixture into a feeding pot for expanding and large-scale propagation; culturing for 5-7 days;
step eight: and detecting the number of the amblyseius barkeri in each gram of the wheat bran in the seventh step, wherein the number of the amblyseius barkeri in each gram of the wheat bran reaches 250, and packaging for use or further propagation.
2. The simple rearing method of Amblyseius barkeri as claimed in claim 1, characterized in that: in the second step, the thickness of wheat bran on the conveyor belt is below 1cm, the temperature in the cabinet is 160 ℃, the time from the cabinet entry to the cabinet exit is about 10min, and the outlet is connected with a plastic barrel sterilized by ultraviolet rays.
3. The simple rearing method of Amblyseius barkeri as claimed in claim 1, characterized in that: the culture temperature in the fourth step is 28 ℃ and the Relative Humidity (RH) is adjusted to 90%.
4. The simple rearing method of Amblyseius barkeri as claimed in claim 1, characterized in that: the culture temperature in the fifth step is 28 ℃ and RH (relative humidity) is 90%.
5. The simple rearing method of Amblyseius barkeri as claimed in claim 1, characterized in that: the culturing of the seventh step is carried out under the conditions of a temperature of 28 ℃ and an RH (relative humidity) of 90%.
6. The simple rearing method of Amblyseius barkeri as claimed in claim 1, characterized in that: and the quantity detection of the sixth step and the eighth step adopts a viewing mirror for observation and detection, and the A4 paper after ultraviolet sterilization and disinfection is used as a base surface for placing mites.
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| JPH0840814A (en) * | 1994-07-28 | 1996-02-13 | Noyaku Bio Technol Kaihatsu Gijutsu Kenkyu Kumiai | Method for multiplying mites |
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| CN105794725A (en) * | 2016-03-24 | 2016-07-27 | 首伯农(北京)生物技术有限公司 | Method for breeding predatory mites and prey mites thereof |
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