CN1115198C - 在载体和覆盖层之间具有中间层的毛细管活性测试元件 - Google Patents
在载体和覆盖层之间具有中间层的毛细管活性测试元件 Download PDFInfo
- Publication number
- CN1115198C CN1115198C CN98813487A CN98813487A CN1115198C CN 1115198 C CN1115198 C CN 1115198C CN 98813487 A CN98813487 A CN 98813487A CN 98813487 A CN98813487 A CN 98813487A CN 1115198 C CN1115198 C CN 1115198C
- Authority
- CN
- China
- Prior art keywords
- sample
- capillary
- canal
- groove
- carrier
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 238000012360 testing method Methods 0.000 title abstract description 48
- 238000000034 method Methods 0.000 claims abstract description 13
- 230000000694 effects Effects 0.000 claims description 17
- 239000000463 material Substances 0.000 claims description 14
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 14
- 239000012530 fluid Substances 0.000 claims description 7
- 230000010148 water-pollination Effects 0.000 claims description 6
- PNEYBMLMFCGWSK-UHFFFAOYSA-N aluminium oxide Inorganic materials [O-2].[O-2].[O-2].[Al+3].[Al+3] PNEYBMLMFCGWSK-UHFFFAOYSA-N 0.000 claims description 5
- 230000015572 biosynthetic process Effects 0.000 claims description 3
- 239000000284 extract Substances 0.000 claims description 2
- 239000007788 liquid Substances 0.000 abstract description 20
- 238000005070 sampling Methods 0.000 abstract 1
- 239000000523 sample Substances 0.000 description 86
- 239000010408 film Substances 0.000 description 23
- 229920003023 plastic Polymers 0.000 description 12
- 239000004033 plastic Substances 0.000 description 12
- 238000001514 detection method Methods 0.000 description 10
- 210000004369 blood Anatomy 0.000 description 8
- 239000008280 blood Substances 0.000 description 8
- 229910052751 metal Inorganic materials 0.000 description 8
- 239000002184 metal Substances 0.000 description 8
- 238000004519 manufacturing process Methods 0.000 description 7
- 230000002209 hydrophobic effect Effects 0.000 description 6
- -1 polypropylene Polymers 0.000 description 6
- 230000005660 hydrophilic surface Effects 0.000 description 5
- 230000003647 oxidation Effects 0.000 description 5
- 238000007254 oxidation reaction Methods 0.000 description 5
- 238000004080 punching Methods 0.000 description 5
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 4
- 239000004698 Polyethylene Substances 0.000 description 4
- 239000002390 adhesive tape Substances 0.000 description 4
- 238000006243 chemical reaction Methods 0.000 description 4
- 239000008103 glucose Substances 0.000 description 4
- 229920000573 polyethylene Polymers 0.000 description 4
- 230000001154 acute effect Effects 0.000 description 3
- 230000008859 change Effects 0.000 description 3
- 239000003153 chemical reaction reagent Substances 0.000 description 3
- 239000011248 coating agent Substances 0.000 description 3
- 238000000576 coating method Methods 0.000 description 3
- 230000000007 visual effect Effects 0.000 description 3
- 239000004952 Polyamide Substances 0.000 description 2
- 239000004743 Polypropylene Substances 0.000 description 2
- 239000004793 Polystyrene Substances 0.000 description 2
- 239000004411 aluminium Substances 0.000 description 2
- 229910052782 aluminium Inorganic materials 0.000 description 2
- XAGFODPZIPBFFR-UHFFFAOYSA-N aluminium Chemical compound [Al] XAGFODPZIPBFFR-UHFFFAOYSA-N 0.000 description 2
- 230000005540 biological transmission Effects 0.000 description 2
- 239000011521 glass Substances 0.000 description 2
- 229920002647 polyamide Polymers 0.000 description 2
- 229920001155 polypropylene Polymers 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 239000010409 thin film Substances 0.000 description 2
- 238000009736 wetting Methods 0.000 description 2
- 229920002319 Poly(methyl acrylate) Polymers 0.000 description 1
- XTXRWKRVRITETP-UHFFFAOYSA-N Vinyl acetate Chemical class CC(=O)OC=C XTXRWKRVRITETP-UHFFFAOYSA-N 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 239000012491 analyte Substances 0.000 description 1
- 239000000538 analytical sample Substances 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 210000001124 body fluid Anatomy 0.000 description 1
- 239000010839 body fluid Substances 0.000 description 1
- 239000007767 bonding agent Substances 0.000 description 1
- 239000012876 carrier material Substances 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 239000013039 cover film Substances 0.000 description 1
- 206010012601 diabetes mellitus Diseases 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000000835 electrochemical detection Methods 0.000 description 1
- 230000005518 electrochemistry Effects 0.000 description 1
- 230000002349 favourable effect Effects 0.000 description 1
- 238000011010 flushing procedure Methods 0.000 description 1
- 239000011888 foil Substances 0.000 description 1
- 125000002791 glucosyl group Chemical group C1([C@H](O)[C@@H](O)[C@H](O)[C@H](O1)CO)* 0.000 description 1
- 230000003699 hair surface Effects 0.000 description 1
- 229920001477 hydrophilic polymer Polymers 0.000 description 1
- 230000005661 hydrophobic surface Effects 0.000 description 1
- 230000008676 import Effects 0.000 description 1
- 230000001788 irregular Effects 0.000 description 1
- 238000003475 lamination Methods 0.000 description 1
- 230000000873 masking effect Effects 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 150000002739 metals Chemical class 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 230000035515 penetration Effects 0.000 description 1
- 210000002381 plasma Anatomy 0.000 description 1
- 239000004800 polyvinyl chloride Substances 0.000 description 1
- 210000003296 saliva Anatomy 0.000 description 1
- 210000002966 serum Anatomy 0.000 description 1
- 238000010008 shearing Methods 0.000 description 1
- 210000004243 sweat Anatomy 0.000 description 1
- 230000036962 time dependent Effects 0.000 description 1
- 210000002700 urine Anatomy 0.000 description 1
- 239000000080 wetting agent Substances 0.000 description 1
Images
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/52—Use of compounds or compositions for colorimetric, spectrophotometric or fluorometric investigation, e.g. use of reagent paper and including single- and multilayer analytical elements
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5023—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures with a sample being transported to, and subsequently stored in an absorbent for analysis
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/02—Adapting objects or devices to another
- B01L2200/026—Fluid interfacing between devices or objects, e.g. connectors, inlet details
- B01L2200/027—Fluid interfacing between devices or objects, e.g. connectors, inlet details for microfluidic devices
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0809—Geometry, shape and general structure rectangular shaped
- B01L2300/0825—Test strips
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0887—Laminated structure
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2400/00—Moving or stopping fluids
- B01L2400/04—Moving fluids with specific forces or mechanical means
- B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
- B01L2400/0406—Moving fluids with specific forces or mechanical means specific forces capillary forces
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/00029—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor provided with flat sample substrates, e.g. slides
- G01N2035/00099—Characterised by type of test elements
- G01N2035/00108—Test strips, e.g. paper
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T436/00—Chemistry: analytical and immunological testing
- Y10T436/25—Chemistry: analytical and immunological testing including sample preparation
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T436/00—Chemistry: analytical and immunological testing
- Y10T436/25—Chemistry: analytical and immunological testing including sample preparation
- Y10T436/25375—Liberation or purification of sample or separation of material from a sample [e.g., filtering, centrifuging, etc.]
- Y10T436/255—Liberation or purification of sample or separation of material from a sample [e.g., filtering, centrifuging, etc.] including use of a solid sorbent, semipermeable membrane, or liquid extraction
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Hematology (AREA)
- Analytical Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Clinical Laboratory Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Immunology (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Urology & Nephrology (AREA)
- Biochemistry (AREA)
- General Physics & Mathematics (AREA)
- Cell Biology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Pathology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Sampling And Sample Adjustment (AREA)
- Investigating Or Analyzing Non-Biological Materials By The Use Of Chemical Means (AREA)
- Measurement Of The Respiration, Hearing Ability, Form, And Blood Characteristics Of Living Organisms (AREA)
- Automatic Analysis And Handling Materials Therefor (AREA)
- Devices For Use In Laboratory Experiments (AREA)
- Analysing Materials By The Use Of Radiation (AREA)
Abstract
本发明涉及一种为分析元件收集液体试样样品的装置，其中通过毛细管活性孔道将试样从取样位置传输到测定位置。毛细管活性孔道基本上是由载体、覆盖层和位于覆盖层和载体之间的任选的中间层形成的，在分析测试元件的边缘上，在构成能传输液体的毛细管孔道的表面之一上，在形成加入试样开口的测试元件的边缘处有一个凹槽，以使形成施加试样开口的测试元件的边缘的一侧至少一部分是断开的，并露出对着凹槽表面。本发明还涉及借助于本发明的装置将液体试样吸入分析元件中的方法。
Description
本发明涉及为分析测试元件抽取分析试样样品的装置，其中在分析元件的毛细管活性孔道中将试样从施加试样的开口传输到试样的测定位置，其中毛细管的活性孔道基本上是由载体、覆盖层和在覆盖层与载体之间的任选的中间层形成的，此外，本发明还涉及借助于所述的装置将液体试样抽入分析元件的方法。
时常采用所谓的载体束缚测试来定性或定量地分析测定体液，特别是血液中的成分。在这些测试中，试剂被埋在与试样接触的相应的固态载体层中。如果有目标分析物存在，液体试样与试剂的反应会产生可检测的信号，特别是可用目视或借助于仪器测定的颜色变化，通常采用反射光度计测定。
测试元件或测试载体往往呈试条的形式，试条基本上由塑料材料制成的细长的载体层，和作为测试区施加到其上的检测层组成。然而，已知有方形或矩形小片状的测试载体。
采用目视或反射光度计测定的用于门诊诊断的测试元件，往往象电化学传感器和生物传感器那样，结构成施加试样区和检测区按竖直轴方向一个位于另一个之上。这种结构形式是有问题的。在必须将加上试样的试条插入仪器例如反射光度计进行测定时，可传染的试样物质能与仪器的部件发生接触，并可能污染这些部件。而且，特别是在未经训练的人员，例如糖尿病患者在自控血糖时使用试条的情况下，实现体积定量是有困难的。
最近可以买到一些具有毛细管孔道或间隙的测试元件，借助于这些测试元件，可以解决至少一些所述的问题。
英国专利-B-0 034 049涉及一种测试元件，其中将试样施加到中央的施加试样位置，例如覆盖层上的开口，试样由毛细管作用力传输到在空间上与试样施加位置分开的几个检测区。在这种情况下，值得注意的是，在英国专利-B-0 010 456中还叙述到施加试样的开口具有专门设计的几何形状，而且将这种专门设计看作是特别优选的。主张将顶视图中试样进口的正六边形应是开口中的试样液滴的中心。这能促进试样渗入与施加试样开口呈垂直的毛细管活性孔道。
在所述的毛细管孔隙测试元件中是通过测试元件中与毛细管孔隙垂直的开口施加试样，而在另一种设计中，将试样液体直接施加到与展开方向平行的毛细管间隙中。这能通过具有毛细管孔道终止边缘的测试元件，并用此边缘与试样液体直接接触，并通过能传输液体的毛细管孔道吸收试样来最简单地实现。
后一种测试元件经常出现的问题是，施加到毛细管孔隙施加试样开口上的液滴不能渗入间隙。这种现象可能有不同的原因。可以想象到，在这类测试元件的制造中，由于制造上的原因，开口达不到试样液滴进入毛细管孔道所要求的尺寸，例如因为在测试元件经截取、剪切或冲孔时，开口已被沾污或压扁。另一个原因可能是所用材料的疏水性，这些疏水性材料例如疏水性塑料常用于制造所述的测试元件，它削弱、延迟或阻止试样渗入毛细管孔隙。例如如果其内表面确实是亲水性的，但由于所用材料的剪切边缘是疏水性的，则液滴已经不能进入毛细管孔道内或只能非常缓慢地进入。
本发明的目的是要消除现有技术的缺点。
这一点是通过在本专利的权利要求中所表征的主题实现的。
本发明涉及为分析元件抽取液体试样的装置，其中在毛细管孔道中将试样从施加试样的开口传输到在分析元件中测定试样的位置，其中毛细管活性孔道基本上是由载体、覆盖层和在覆盖层与载体之间的任选的中间层形成的，该装置的特征在于，在形成能传输液体的毛细管孔道的一个表面上，在分析测试元件的形成施加试样的开口边缘处，有一凹槽，所以在该装置形成施加试样开口的边缘的一侧，至少有一部分被中断，露出对着凹槽的表面。
根据本发明的装置，特别优选包含一个这样的凹槽。然而，也可采用其它的设计形式，其中在一个表面上有几个、至少有二个凹槽一起存在，或错开在相反的面上。对凹槽的形状没有任何限制，只要形成施加试样开口的边缘的至少一部分被凹槽至少部分断开。因而三角形的或多边形的以及圆形的或椭圆形的都是可行的。也不排除不规则的形状。
在测试元件的边缘，在形成毛细管孔道的表面上的形成试样加入口的凹槽，用于确保试样液体能够进入毛细管孔道。这是使试样液滴在以凹槽断开的装置的最接近样品施加开口的边缘处直接施加到表面上实现的，该表面的伸展，形成毛细管的内表面。适当地选择凹槽的几何形状和尺寸，确保液滴与毛细管活性区发生接触的几率非常高，而且与投药的准确位置无关，又确保液滴易吸入毛细管内。例如，应当这样地选择露出表面的尺寸，使被施加到其上的液滴至少在一个位置与毛细管活性区发生接触。例如，应当如此地选择凹槽的一维尺寸，例如宽度，使液滴直径略大于凹槽所选的该维尺寸。已经证明，对于3μl的液滴，凹槽的宽度为1mm是适宜的。特别优选由凹槽露出区域是亲水性来将试样液滴吸入毛细管孔道，该区域至少在毛细管传输孔道方向上直接与毛细管活性区邻接。
在这种情况下，亲水性表面意是吸水的表面。水性试样，也包括血液，能在这类表面上很好地展开。此外，这类表面的特征还在于，置于其上的水滴，在界面上形成的边界角或接触角是锐角。相反地，在疏水性即斥水性的表面上，在水滴和表面之间的界面上形成的边界角是钝角。
由测试液体和被检测表面的表面张力形成的边界角，是表面亲水性的量度。例如水的表面张力为72mN/m。如果被观测表面的表面张力值比该值低很多，即大于20mN/m，则其润湿作用差，得到的边界角是钝角。这样的表面被称作疏水性表面。如果表面张力接近水的表面张力值，则其润湿性好，边界角是锐角。相反，如果表面张力等于或大于水的表面张力值，那么液滴就能渗开，所以液体会完全展开。因而不能再测定其边界角。以水滴形成的边界角是锐角的表面或在其上观测到水滴完全展开的表面，被称作亲水性表面。
毛细管吸收液体的能力，取决于液体对孔道表面的润湿性。对于水性试样，这意味着毛细管应由表面张力近达到72mN/m或超过该值的材料制造。
制造能迅速地吸收水性试样的毛细管的充分亲水性的材料是例如玻璃、金属或陶瓷。然而，这些材料不适合在测试载体中使用，因为它们有一些严重的缺点，例如玻璃或陶瓷有破碎的问题，而许多金属，其表面性质有随时间变化的危险。因此通常采用塑料薄膜或模制件制造测试元件。一般所用塑料的表面张力几乎不超过45mN/m。即使采用相对说来最亲水的塑料，例如聚甲基丙稀酸甲酯(PMMA)或聚酰胺(PA)，如果采用它们，只能制造缓慢吸收的毛细管。由例如聚苯乙烯(PS)、聚丙烯(PP)或聚乙烯(PE)之类疏水性塑料制造的毛细管，基本上不吸收水性试样。因此，对于具有毛细管活性孔道的测试元件，必须赋予用作结构材料的塑料以亲水性，即使它们亲水化。
在根据本发明的分析测试元件优选的实施方案中，至少一个，但优选二个，特别优选二个相对的表面是亲水的，它们形成能够传输液体的毛细管孔道的内表面。非常优选至少对着凹槽的露出表面是亲水的。如果一个以上的表面是亲水性的，则这些表面可采用相同或不同的方法制成亲水性的。在形成毛细管活性孔道的材料，特别是载体本身是疏水性的或只有非常轻微的亲水性时，亲水化是特别必要的，因为它们是由例如非极性的塑料组成的。采用例如聚苯乙烯(PS)、聚乙烯(PE)、聚对本二酸乙烯酯(PET)或聚氯乙烯(PVC)之类的非极性塑料作为载体材料是有利的，因为它们不吸收被检测的液体，因此检测层能有效地利用试样体积。毛吸管孔道表面的亲水化，能使极性的，优选水性的试样液体易进入毛细管孔道，并迅速传输到试样测定位置。在这种情况下，为得到所要求的结果，测定位置被认为是在分析元件中应将试样传输到的位置或区域。如果分析元件是例如用光学或用光度计测定的测试载体，则试样的测定位置就是测试载体的检测区，其中具有颜色变化的反应是可观测的。如果分析元件是电化学传感器，则该试样测定位置就被认为是并入传感器中的电极，它能检测所要求的电化学检测反应。如果分析元件本身不用于检测试样中的被分析物，而是例如只用于体积配量或吸收一定量的试样材料，则试样的测定位置可以是分析元件上一个简单的记号，达到这个记号毛细管孔隙必定被充满，以便例如确定某个最小的试样体积。
在制造过程中，采用亲水性材料实现毛细管孔道表面的亲水化是理想的，然而，亲水性材料本身不能吸收试样液体，或只吸收到可忽略不计的程度。在不可能采用亲水性材料的情况下，可采用具有稳定的对试样液体是惰性的亲水层的适宜覆层，使疏水性的或只有微弱亲水性的表面亲水化，例如，通过施加含润湿剂的层，或借助于溶胶-凝胶技术采用毫微米复合物覆盖表面的方法，将光致反应的亲水性聚合物共价结合到塑料表面上。此外，通过表面的热、物理或化学处理来提高亲水性也是可行的。
特别优选采用氧化铝薄层进行亲水化。例如可通过给工件真空涂敷金属铝，然后氧化该金属，或采用用金属薄膜或覆金属的塑料膜制造测试载体，将这些层直接施加到所需的测试元件部件上，为了达到所需的亲水性，金属薄膜或覆金属的塑料也必须被氧化。在这种情况下，金属层的厚度为1-500nm是足够的。然后将金属层氧化成氧化的形式，对此，已经证明，除了电化学或阳极氧化方法以外，在水蒸气存在下或在水中煮沸进行上述所有的氧化是特别适宜的方法。以这种方法制备的氧化物层，视方法而定其厚度为0.1-500nm，优选10-100nm。在实践中，原则上可以得到层厚较大的金属层以及氧化物层，但没有任何附加的有利作用。
本发明的第二个主题涉及借助于根据本发明的装置抽取液体试样，特别是体液如血液、血浆、血清、尿、唾液和汗等的方法。在本方法中，首先使液体试样在被凹槽所断开的施加试样开口的边缘上与装置接触。通过能传输液体的毛细管孔道中的毛吸作用力将试样液体传输到装置的内部，使其能够达到它的测定位置。
通过图1和2以及下列实施例更详细地说明本发明。
图1示出根据本发明的装置的特别优选的实施方案。在图1A中示出根据本发明的装置的顶视示意图，图1B-1G分别示出沿线A-A′(1B)、B-B′(1C)、C-C′(1D和1G)、D-D′(1E)和E-E′(1F)的截面图。
图2示出根据本发明的装置的施加试样区的详细放大投影图。
图中的数字表示：
1 载体
2 检测元件
3 毛细管孔道
4 施加试样的开口
5 施加试样的凹槽
6 排气口
7 覆盖层
8 间隙覆盖薄膜
9 中间层
10 支撑薄膜
在图1中用示意图示出根据本发明的装置特别优选实施方案的各个视图(图1A-1F)。试图通过所示的各个视图给出根据本发明装置的三维概念。中间层(9)例如以双面胶带的形式固定在惰性载体(1)上。中间层(9)在毛细管孔道(3)的区域内有一个凹槽，凹槽确定了孔道(3)的长度和宽度。孔道的高度是由中间层(9)的厚度给出的。在毛细管孔道(3)对着载体(1)的一侧上，覆盖层(7)例如塑料薄膜，与检测元件(2)例如浸渍试剂的膜邻接。为了确保检测元件和覆盖层之间毛细管的连续性，设有间隙覆盖薄膜(8)。该薄膜可被亲水化，以便能将试样从施加试样的开口(4)迅速传输到排气口(6)，排气口(6)确定了毛细管孔道相对端。亲水化的另一个优点是，可将试样液滴直接施加到凹槽(5)的区域内亲水性的表面上，凹槽(5)在几个边界面上被毛细管活性区(3)所包围。这能使液滴迅速地渗入测试元件。
毛细管区(3)从施加试样的开口(4)伸展到检测元件(2)的相对端，因此，它能确保试样液体与检测元件(2)的整个区域接触，因而试样均匀地分布在检测元件(2)上。施加试样的开口(4)和排气口(6)在毛细管传输方向上界定了毛细管活性区(3)。
为了覆盖胶带露出的区域，图1G示出是如何用支撑薄膜(10)覆盖中间层(9)的。然而，排气口(6)不得被覆盖。
在采用所示的装置时，其施加试样的开口(4)例如与指尖上的血滴接触。在这种情况下，血滴是从上面即载体(1)的平面，还是从前面即从测试元件的包含的施加试样开口(4)正面与根据本发明的装置接触并不重要。这基本上消除了习惯于使用从上方投放试样的常规试条的用户所预计的施加试样的错误。在使用根据本发明的装置时，血滴通过载体(1)上的凹槽(5)与露出的任选的亲水性表面及同时与毛细管孔道(3)发生接触。毛细管孔道被试样填充，直至试样从施加试样的开口(4)填充到排气口(6)为止。然后将该装置从患者的手指上移开，该装置确保仅在毛细管孔道(3)中存在的试样能用于检测元件(2)。因此排除了试样的过量。被界定的毛细管孔道高度界定了在检测元件上的试样层厚度。
在图2示出根据本发明装置的测试元件特别优选的实施方案施加试样区的详细放大投影图。载体(1)上的凹槽(5)，能促进试样液体从施加试样的开口(4)渗入毛细管活性区(3)，在这种情况下，毛细管活性区(3)是由载体(1)、中间层(9)和覆盖层(7)形成的。除了所示的形状以外，凹槽还可具有用于根据本发明的目的所要求的任何其它形状。其中采用半圆形、三角形或多边形以及采用一个或几个紧挨着的或错开相对的凹槽是可行的。
实施例1
根据本发明的分析测试元件的制造
将100μm厚的双面胶带粘在350μm厚的聚对苯二酸乙烯酯薄膜(Melinex，帝国化学工业公司，德国缅因河畔法兰克福)上，该薄膜上覆有30nm厚的用水蒸气完全氧化的铝层。薄膜的长度为25mm，宽度为5mm。中间的切口形凹槽，宽度为1mm，长度为2mm，位于一个短边上。胶带上冲孔的宽度为2mm，长度大于15mm，该冲孔界定了毛细管孔道的尺寸。选择冲孔的长度略大于所需毛细管活性孔道的长度，而活性孔道的长度是由其覆盖层决定的，以确保在填充试样液体过程中孔道的排气。将3mm长、5mm宽的检测膜粘到胶带的一侧上，该侧在离冲孔末端1mm的距离提供排气。采用从德国专利申请P19629656.0中已知用膜作为检测膜。检测膜是检测葡萄糖专用的。将长12mm、宽5mm的覆盖层粘到胶带在切口形凹槽和检测膜之间仍然敞开的区域上，使覆盖层和检测膜互相毗邻。覆盖层由150μm厚的聚对苯二酸乙烯酯薄膜组成，在其一侧上具有粘接剂，将6μm厚的覆有30nm厚的氧化铝层的聚对苯二酸乙烯酯薄膜(二者为：Hostaphan，Hoechst，德国缅因河畔法兰克福)粘在面对毛细管孔道的一侧。在这种情况下，较薄薄膜的伸展超过面对检测膜这侧上的较厚薄膜约500μm。在将覆盖层安装在胶带上时，必须小心地将较薄薄膜的伸出端铺在检测元件和覆盖层较厚的薄膜之间。为了覆盖仍露出的粘接带的区域，采用175μm厚的Melinex薄膜覆盖，然而，不能覆盖功能区。
以这种方法制得的测试元件具有长15mm、宽2mm和高0.1mm的毛细管孔道。该孔道可吸收3μl试样液体。由该试样润湿的检测膜面积为3mm×2mm。
实施例2
借助于实施例1的测试元件测定血液的葡萄糖浓度
将实施例1的测试元件的施加试样的一侧放在试样液滴上。测试元件的毛细管在2秒内自动地充满试样。如果在试样中有葡萄糖存在，在几秒钟后在检测膜上会看见颜色变化。在约30-35秒后达到反应终点。所得的颜色与试样的葡萄糖浓度有关，既可用目视也可用反射光度计测定。
Claims (7)
1、一种为分析元件抽取液体试样样品的装置，其中在毛细管活性孔道(3)中，使试样从取样位置传输到测定位置，其中毛细管活性孔道(3)，基本上是由载体(1)、覆盖层(7)和在第二覆盖层(7)与载体(1)之间的中间层(9)形成的，其特征在于，呈部分切口形状的凹槽(5)位于形成能传输液体的毛细管孔道(3)的一个表面上，在该装置具有施加试样开口的侧面，其中切口开始于该装置的侧面，并指向毛细管孔道方向的取样位置，以露出对着凹槽(5)的表面，其中形成能传输液体的毛细管孔道内表面的至少一个表面是亲水性的。
2、权利要求1的装置，其特征在于，至少有二个互相紧挨着的凹槽。
3、权利要求1的装置，其特征在于，至少二个凹槽错开地排列在相对侧。
4、权利要求1的装置，其特征在于，对着凹槽的表面是亲水性的。
5、权利要求1或4的装置，其特征在于，亲水化是通过采用亲水材料或采用亲水层覆盖亲水性差的材料达到的。
6、权利要求5的装置，其特征在于，亲水化采用氧化铝层。
7、一种借助于权利要求1-6任一项的装置将液体试样抽入分析元件中的方法，其中使液体试样在以凹槽断开的侧面与其接触，并通过毛细作用力将其传输到能传输液体的毛细管孔道中。
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE19753850.9 | 1997-12-04 | ||
| DE19753850A DE19753850A1 (de) | 1997-12-04 | 1997-12-04 | Probennahmevorrichtung |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1290195A CN1290195A (zh) | 2001-04-04 |
| CN1115198C true CN1115198C (zh) | 2003-07-23 |
Family
ID=7850758
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN98813487A Expired - Lifetime CN1115198C (zh) | 1997-12-04 | 1998-12-03 | 在载体和覆盖层之间具有中间层的毛细管活性测试元件 |
Country Status (15)
| Country | Link |
|---|---|
| US (2) | US7238534B1 (zh) |
| EP (1) | EP1035920B1 (zh) |
| JP (1) | JP3418173B2 (zh) |
| KR (1) | KR20010032723A (zh) |
| CN (1) | CN1115198C (zh) |
| AT (1) | ATE220953T1 (zh) |
| AU (1) | AU742823B2 (zh) |
| CA (1) | CA2310762C (zh) |
| DE (2) | DE19753850A1 (zh) |
| ES (1) | ES2180229T3 (zh) |
| HK (1) | HK1035875A1 (zh) |
| HU (1) | HUP0004513A3 (zh) |
| PL (1) | PL340913A1 (zh) |
| TW (1) | TW396273B (zh) |
| WO (1) | WO1999029428A1 (zh) |
Families Citing this family (90)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE19753850A1 (de) * | 1997-12-04 | 1999-06-10 | Roche Diagnostics Gmbh | Probennahmevorrichtung |
| DE19753848A1 (de) * | 1997-12-04 | 1999-06-10 | Roche Diagnostics Gmbh | Modifikation von Oberflächen zur Steigerung der Oberflächenspannung |
| US6391005B1 (en) | 1998-03-30 | 2002-05-21 | Agilent Technologies, Inc. | Apparatus and method for penetration with shaft having a sensor for sensing penetration depth |
| WO2001002093A2 (en) † | 1999-07-07 | 2001-01-11 | 3M Innovative Properties Company | Detection article having fluid control film with capillary channels |
| US8641644B2 (en) | 2000-11-21 | 2014-02-04 | Sanofi-Aventis Deutschland Gmbh | Blood testing apparatus having a rotatable cartridge with multiple lancing elements and testing means |
| WO2002084291A1 (fr) * | 2001-04-12 | 2002-10-24 | Arkray, Inc. | Instrument d'analyse de prelevement |
| DE60238119D1 (de) | 2001-06-12 | 2010-12-09 | Pelikan Technologies Inc | Elektrisches betätigungselement für eine lanzette |
| US9795747B2 (en) | 2010-06-02 | 2017-10-24 | Sanofi-Aventis Deutschland Gmbh | Methods and apparatus for lancet actuation |
| JP4209767B2 (ja) | 2001-06-12 | 2009-01-14 | ペリカン テクノロジーズ インコーポレイテッド | 皮膚の性状の一時的変化に対する適応手段を備えた自動最適化形切開器具 |
| AU2002348683A1 (en) | 2001-06-12 | 2002-12-23 | Pelikan Technologies, Inc. | Method and apparatus for lancet launching device integrated onto a blood-sampling cartridge |
| JP4841063B2 (ja) * | 2001-06-12 | 2011-12-21 | テクノクオーツ株式会社 | マイクロチャンネル構造体およびその製造方法 |
| US8337419B2 (en) | 2002-04-19 | 2012-12-25 | Sanofi-Aventis Deutschland Gmbh | Tissue penetration device |
| US9226699B2 (en) | 2002-04-19 | 2016-01-05 | Sanofi-Aventis Deutschland Gmbh | Body fluid sampling module with a continuous compression tissue interface surface |
| US7981056B2 (en) | 2002-04-19 | 2011-07-19 | Pelikan Technologies, Inc. | Methods and apparatus for lancet actuation |
| US7041068B2 (en) | 2001-06-12 | 2006-05-09 | Pelikan Technologies, Inc. | Sampling module device and method |
| US9427532B2 (en) | 2001-06-12 | 2016-08-30 | Sanofi-Aventis Deutschland Gmbh | Tissue penetration device |
| CN1300578C (zh) * | 2001-07-27 | 2007-02-14 | 爱科来株式会社 | 分析用具 |
| US7640047B2 (en) * | 2001-09-11 | 2009-12-29 | Arkray, Inc. | Test instrument, attachment, and concentration measuring apparatus |
| US8372016B2 (en) | 2002-04-19 | 2013-02-12 | Sanofi-Aventis Deutschland Gmbh | Method and apparatus for body fluid sampling and analyte sensing |
| US8702624B2 (en) | 2006-09-29 | 2014-04-22 | Sanofi-Aventis Deutschland Gmbh | Analyte measurement device with a single shot actuator |
| US7229458B2 (en) | 2002-04-19 | 2007-06-12 | Pelikan Technologies, Inc. | Method and apparatus for penetrating tissue |
| US7297122B2 (en) | 2002-04-19 | 2007-11-20 | Pelikan Technologies, Inc. | Method and apparatus for penetrating tissue |
| US8784335B2 (en) | 2002-04-19 | 2014-07-22 | Sanofi-Aventis Deutschland Gmbh | Body fluid sampling device with a capacitive sensor |
| US9314194B2 (en) | 2002-04-19 | 2016-04-19 | Sanofi-Aventis Deutschland Gmbh | Tissue penetration device |
| US8221334B2 (en) | 2002-04-19 | 2012-07-17 | Sanofi-Aventis Deutschland Gmbh | Method and apparatus for penetrating tissue |
| US7331931B2 (en) | 2002-04-19 | 2008-02-19 | Pelikan Technologies, Inc. | Method and apparatus for penetrating tissue |
| US7892183B2 (en) | 2002-04-19 | 2011-02-22 | Pelikan Technologies, Inc. | Method and apparatus for body fluid sampling and analyte sensing |
| US8360992B2 (en) | 2002-04-19 | 2013-01-29 | Sanofi-Aventis Deutschland Gmbh | Method and apparatus for penetrating tissue |
| US7232451B2 (en) | 2002-04-19 | 2007-06-19 | Pelikan Technologies, Inc. | Method and apparatus for penetrating tissue |
| US7976476B2 (en) | 2002-04-19 | 2011-07-12 | Pelikan Technologies, Inc. | Device and method for variable speed lancet |
| US9248267B2 (en) | 2002-04-19 | 2016-02-02 | Sanofi-Aventis Deustchland Gmbh | Tissue penetration device |
| US7547287B2 (en) | 2002-04-19 | 2009-06-16 | Pelikan Technologies, Inc. | Method and apparatus for penetrating tissue |
| US7674232B2 (en) | 2002-04-19 | 2010-03-09 | Pelikan Technologies, Inc. | Method and apparatus for penetrating tissue |
| US7175642B2 (en) | 2002-04-19 | 2007-02-13 | Pelikan Technologies, Inc. | Methods and apparatus for lancet actuation |
| US9795334B2 (en) | 2002-04-19 | 2017-10-24 | Sanofi-Aventis Deutschland Gmbh | Method and apparatus for penetrating tissue |
| US8579831B2 (en) | 2002-04-19 | 2013-11-12 | Sanofi-Aventis Deutschland Gmbh | Method and apparatus for penetrating tissue |
| US7901362B2 (en) | 2002-04-19 | 2011-03-08 | Pelikan Technologies, Inc. | Method and apparatus for penetrating tissue |
| US8267870B2 (en) | 2002-04-19 | 2012-09-18 | Sanofi-Aventis Deutschland Gmbh | Method and apparatus for body fluid sampling with hybrid actuation |
| US7909778B2 (en) | 2002-04-19 | 2011-03-22 | Pelikan Technologies, Inc. | Method and apparatus for penetrating tissue |
| US7198606B2 (en) | 2002-04-19 | 2007-04-03 | Pelikan Technologies, Inc. | Method and apparatus for a multi-use body fluid sampling device with analyte sensing |
| US7491178B2 (en) | 2002-04-19 | 2009-02-17 | Pelikan Technologies, Inc. | Method and apparatus for penetrating tissue |
| DE10220296A1 (de) * | 2002-05-07 | 2003-11-20 | Roche Diagnostics Gmbh | Vorrichtung zur Probennahme von flüssigen Proben |
| AUPS309002A0 (en) | 2002-06-20 | 2002-07-11 | Vision Biosystems Limited | A covertile for a substrate |
| WO2004033101A1 (en) * | 2002-10-11 | 2004-04-22 | Zbx Corporation | Diagnostic devices |
| US8574895B2 (en) | 2002-12-30 | 2013-11-05 | Sanofi-Aventis Deutschland Gmbh | Method and apparatus using optical techniques to measure analyte levels |
| JP2004333141A (ja) * | 2003-04-30 | 2004-11-25 | Toppan Printing Co Ltd | 液体反応検査具 |
| WO2004107975A2 (en) | 2003-05-30 | 2004-12-16 | Pelikan Technologies, Inc. | Method and apparatus for fluid injection |
| WO2004107964A2 (en) | 2003-06-06 | 2004-12-16 | Pelikan Technologies, Inc. | Blood harvesting device with electronic control |
| WO2006001797A1 (en) | 2004-06-14 | 2006-01-05 | Pelikan Technologies, Inc. | Low pain penetrating |
| US8282576B2 (en) | 2003-09-29 | 2012-10-09 | Sanofi-Aventis Deutschland Gmbh | Method and apparatus for an improved sample capture device |
| DE10346417A1 (de) * | 2003-10-07 | 2005-06-02 | Roche Diagnostics Gmbh | Analytisches Testelement umfassend ein Netzwerk zur Bildung eines Kapillarkanals |
| US9351680B2 (en) | 2003-10-14 | 2016-05-31 | Sanofi-Aventis Deutschland Gmbh | Method and apparatus for a variable user interface |
| US7822454B1 (en) | 2005-01-03 | 2010-10-26 | Pelikan Technologies, Inc. | Fluid sampling device with improved analyte detecting member configuration |
| WO2005065414A2 (en) | 2003-12-31 | 2005-07-21 | Pelikan Technologies, Inc. | Method and apparatus for improving fluidic flow and sample capture |
| DE102004007274A1 (de) | 2004-02-14 | 2005-09-15 | Roche Diagnostics Gmbh | Testelement und Testelementanalysesystem zum Untersuchen einer flüssigen Probe sowie Verfahren zum Steuern der Benetzung eines Testfeldes eines Testelements |
| DE102004009012A1 (de) * | 2004-02-25 | 2005-09-15 | Roche Diagnostics Gmbh | Testelement mit einer Kapillare zum Transport einer flüssigen Probe |
| RU2376603C2 (ru) * | 2004-03-05 | 2009-12-20 | Эгомедикаль Свисс Аг | Тест-система для определения концентрации анализируемого вещества в физиологической жидкости |
| EP1751546A2 (en) | 2004-05-20 | 2007-02-14 | Albatros Technologies GmbH & Co. KG | Printable hydrogel for biosensors |
| US20050266398A1 (en) * | 2004-06-01 | 2005-12-01 | Peter Lea | Method and device for rapid detection and quantitation of macro and micro matrices |
| US9820684B2 (en) | 2004-06-03 | 2017-11-21 | Sanofi-Aventis Deutschland Gmbh | Method and apparatus for a fluid sampling device |
| US9775553B2 (en) | 2004-06-03 | 2017-10-03 | Sanofi-Aventis Deutschland Gmbh | Method and apparatus for a fluid sampling device |
| US7766845B2 (en) | 2004-06-21 | 2010-08-03 | Roche Diagnostics Operations, Inc. | Disposable lancet and lancing cap combination for increased hygiene |
| US8652831B2 (en) | 2004-12-30 | 2014-02-18 | Sanofi-Aventis Deutschland Gmbh | Method and apparatus for analyte measurement test time |
| US7846311B2 (en) | 2005-09-27 | 2010-12-07 | Abbott Diabetes Care Inc. | In vitro analyte sensor and methods of use |
| DE102006025477B4 (de) | 2006-05-30 | 2009-01-15 | Ekf - Diagnostic Gmbh | Küvette und Verfahren zu ihrer Herstellung |
| CA2666887A1 (en) * | 2006-10-18 | 2008-04-24 | Research Development Foundation | Alpha-msh therapies for treatment of autoimmune disease |
| JP4811267B2 (ja) * | 2006-12-22 | 2011-11-09 | パナソニック株式会社 | マイクロチップ及びそれを用いた分析デバイス |
| EP2254699A1 (en) * | 2008-03-11 | 2010-12-01 | Koninklijke Philips Electronics N.V. | Filtering apparatus for filtering a fluid |
| EP2265324B1 (en) | 2008-04-11 | 2015-01-28 | Sanofi-Aventis Deutschland GmbH | Integrated analyte measurement system |
| US9375169B2 (en) | 2009-01-30 | 2016-06-28 | Sanofi-Aventis Deutschland Gmbh | Cam drive for managing disposable penetrating member actions with a single motor and motor and control system |
| KR101110561B1 (ko) * | 2009-06-04 | 2012-02-15 | 주식회사 인포피아 | 바이오 센서 |
| US9216413B2 (en) * | 2009-07-07 | 2015-12-22 | Boehringer Ingelheim Microparts Gmbh | Plasma separation reservoir |
| US8965476B2 (en) | 2010-04-16 | 2015-02-24 | Sanofi-Aventis Deutschland Gmbh | Tissue penetration device |
| US9157903B2 (en) * | 2011-02-25 | 2015-10-13 | Honeywell International Inc. | Microfluidic separation of plasma for colormetric assay |
| KR101215006B1 (ko) * | 2011-08-16 | 2012-12-24 | 바디텍메드 주식회사 | 이중 채취수단을 갖는 모세관 마이크로 큐베트 |
| EP2636750A1 (en) | 2012-03-06 | 2013-09-11 | Roche Diagniostics GmbH | Compatible solute ectoine as well as derivatives thereof for enzyme stabilization |
| JP6111323B2 (ja) | 2012-04-19 | 2017-04-05 | エフ ホフマン−ラ ロッシュ アクチェン ゲゼルシャフト | 血液中の検体濃度を測定する方法および装置 |
| EP2972273B1 (en) | 2013-03-15 | 2020-10-21 | Roche Diabetes Care GmbH | Methods of using information from recovery pulses in electrochemical analyte measurements as well as devices incorporating the same |
| CN105283757B (zh) | 2013-03-15 | 2019-04-23 | 豪夫迈·罗氏有限公司 | 对分析物的电化学测量进行防故障的方法以及结合该方法的设备、装置和系统 |
| KR101727446B1 (ko) | 2013-03-15 | 2017-04-14 | 에프. 호프만-라 로슈 아게 | 바이오센서 알고리즘들을 구성하는데 사용된 데이터를 스케일링하는 방법들 뿐만 아니라 이를 통합한 기기들, 장치들 및 시스템들 |
| EP3388824B1 (en) | 2013-03-15 | 2021-04-14 | Roche Diabetes Care GmbH | Methods of detecting high antioxidant levels during electrochemical measurements and failsafing an analyte concentration therefrom as well as devices and systems incorporting the same |
| WO2014180939A1 (en) | 2013-05-08 | 2014-11-13 | Roche Diagnostics Gmbh | Stabilization of enzymes by nicotinic acid |
| EP2811299A1 (en) | 2013-06-07 | 2014-12-10 | Roche Diagniostics GmbH | Test element for detecting at least one analyte in a body fluid |
| RU2680140C2 (ru) | 2014-01-24 | 2019-02-18 | Ф.Хоффманн-Ля Рош Аг | Способ изготовления однокодовых и бескодовых тест-полосок |
| EP2905618A1 (en) | 2014-02-05 | 2015-08-12 | Roche Diagnostics GmbH | Use of rare metals as key components |
| EP2927319A1 (en) | 2014-03-31 | 2015-10-07 | Roche Diagnostics GmbH | High load enzyme immobilization by crosslinking |
| RU2618845C1 (ru) * | 2015-12-03 | 2017-05-11 | Общество с ограниченной ответственностью "Научно-исследовательский центр "Модифицированные полимеры" | Способ изготовления кюветы для анализа жидких проб с гидрофильной внутренней поверхностью |
| US20180024073A1 (en) * | 2016-07-21 | 2018-01-25 | Ronald Schornstein | Reach-extended test strip |
| CA3035874A1 (en) | 2016-10-05 | 2018-04-12 | F. Hoffmann-La Roche Ag | Detection reagents and electrode arrangements for multi-analyte diagnostic test elements, as well as methods of using the same |
| US20240091779A1 (en) * | 2022-09-19 | 2024-03-21 | Parhelia Biosciences Corporation | Histological Containment System with Cover Pad |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0010456A1 (en) * | 1978-10-25 | 1980-04-30 | EASTMAN KODAK COMPANY (a New Jersey corporation) | Liquid transport device |
Family Cites Families (62)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| BE754658A (fr) | 1969-08-12 | 1971-02-10 | Merck Patent Gmbh | Lamelle indicatrice, se composant d'une matiere capillaire impregnee, absorbante et gainee de feuilles |
| JPS5466194A (en) * | 1977-11-04 | 1979-05-28 | Kuraray Co | Fet sensor |
| US4138216B1 (en) * | 1977-12-23 | 1997-06-17 | Info Chem Inc | Device for monitoring ethylene oxide sterilization process |
| US4250257A (en) * | 1978-08-24 | 1981-02-10 | Technicon Instruments Corporation | Whole blood analyses in porous media |
| US4254083A (en) * | 1979-07-23 | 1981-03-03 | Eastman Kodak Company | Structural configuration for transport of a liquid drop through an ingress aperture |
| DE2910134A1 (de) | 1979-03-15 | 1980-09-25 | Boehringer Mannheim Gmbh | Diagnostisches mittel zum nachweis von bestandteilen von koerperfluessigkeiten |
| US4323536A (en) | 1980-02-06 | 1982-04-06 | Eastman Kodak Company | Multi-analyte test device |
| JPS5731616A (en) | 1980-08-01 | 1982-02-20 | Kyowa Hakko Kogyo Co Ltd | Relaxing agent for muscle comprising benzofurancarboxylic acid amide |
| GB2090659A (en) * | 1981-01-02 | 1982-07-14 | Instrumentation Labor Inc | Analytical device |
| EP0094924A3 (en) * | 1982-05-14 | 1985-01-30 | Astra Meditec AB | Articles exhibiting a biocompatible surface layer and process for providing articles with such a surface layer |
| US4439526A (en) * | 1982-07-26 | 1984-03-27 | Eastman Kodak Company | Clustered ingress apertures for capillary transport devices and method of use |
| SE8305704D0 (sv) | 1983-10-18 | 1983-10-18 | Leo Ab | Cuvette |
| US4900663A (en) * | 1985-09-13 | 1990-02-13 | Environmental Diagnostics, Inc. | Test kit for determining the presence of organic materials and method of utilizing same |
| CA1271399A (en) * | 1986-04-08 | 1990-07-10 | William G. Meathrel | Ethylene oxide monitoring device |
| DE3711418A1 (de) | 1987-04-04 | 1988-10-20 | Agfa Gevaert Ag | Farbfotografisches aufzeichnungsmaterial mit einem kuppler, der eine fotografisch wirksame verbindung freisetzt |
| US5006474A (en) * | 1987-12-16 | 1991-04-09 | Disease Detection International Inc. | Bi-directional lateral chromatographic test device |
| US5126247A (en) | 1988-02-26 | 1992-06-30 | Enzymatics, Inc. | Method, system and devices for the assay and detection of biochemical molecules |
| DE3814370A1 (de) * | 1988-04-28 | 1989-11-09 | Boehringer Mannheim Gmbh | Testtraeger fuer die analyse einer probenfluessigkeit, verfahren zur durchfuehrung einer solchen analyse und herstellungsverfahren |
| US4933092A (en) | 1989-04-07 | 1990-06-12 | Abbott Laboratories | Methods and devices for the separation of plasma or serum from whole blood |
| US4952373A (en) * | 1989-04-21 | 1990-08-28 | Biotrack, Inc. | Liquid shield for cartridge |
| GB8911462D0 (en) * | 1989-05-18 | 1989-07-05 | Ares Serono Res & Dev Ltd | Devices for use in chemical test procedures |
| US4943415A (en) * | 1989-07-14 | 1990-07-24 | Eastman Kodak Company | Grooved cover for test elements |
| US5100627A (en) * | 1989-11-30 | 1992-03-31 | The Regents Of The University Of California | Chamber for the optical manipulation of microscopic particles |
| GB9002856D0 (en) | 1990-02-08 | 1990-04-04 | Alcan Int Ltd | Fluid detection device |
| US5147606A (en) * | 1990-08-06 | 1992-09-15 | Miles Inc. | Self-metering fluid analysis device |
| US5208163A (en) * | 1990-08-06 | 1993-05-04 | Miles Inc. | Self-metering fluid analysis device |
| US5173433A (en) * | 1990-10-11 | 1992-12-22 | Toxi-Lab Incorporated | Method for chemical analysis |
| US5271895A (en) * | 1991-02-27 | 1993-12-21 | Boehringer Mannheim Corporation | Test strip |
| US5264103A (en) * | 1991-10-18 | 1993-11-23 | Matsushita Electric Industrial Co., Ltd. | Biosensor and a method for measuring a concentration of a substrate in a sample |
| US6017696A (en) * | 1993-11-01 | 2000-01-25 | Nanogen, Inc. | Methods for electronic stringency control for molecular biological analysis and diagnostics |
| JP3382632B2 (ja) * | 1992-03-13 | 2003-03-04 | オリンパス光学工業株式会社 | 生体関連物質の測定方法およびそれに用いる反応容器 |
| US5308775A (en) * | 1992-09-15 | 1994-05-03 | Abbott Laboratories | Assay devices for concurrently detecting an analyte and confirming the test result |
| FR2701117B1 (fr) * | 1993-02-04 | 1995-03-10 | Asulab Sa | Système de mesures électrochimiques à capteur multizones, et son application au dosage du glucose. |
| ES2105678T3 (es) * | 1993-03-17 | 1997-10-16 | Akzo Nobel Nv | Dispositivo de deteccion de una substancia de reaccion especifica. |
| US5843691A (en) * | 1993-05-15 | 1998-12-01 | Lifescan, Inc. | Visually-readable reagent test strip |
| US5405511A (en) * | 1993-06-08 | 1995-04-11 | Boehringer Mannheim Corporation | Biosensing meter with ambient temperature estimation method and system |
| DE4427363A1 (de) * | 1993-08-03 | 1995-03-09 | A & D Co Ltd | Chemischer Einmalsensor |
| US5384030A (en) * | 1994-02-15 | 1995-01-24 | General Motors Corporation | Exhaust sensor including a composite tile sensing element and methods of making the same |
| US5762770A (en) * | 1994-02-21 | 1998-06-09 | Boehringer Mannheim Corporation | Electrochemical biosensor test strip |
| US5437999A (en) * | 1994-02-22 | 1995-08-01 | Boehringer Mannheim Corporation | Electrochemical sensor |
| JP3027306B2 (ja) * | 1994-06-02 | 2000-04-04 | 松下電器産業株式会社 | バイオセンサおよびその製造方法 |
| CA2215346C (en) | 1995-03-14 | 2003-05-27 | Howard Milne Chandler | Sample collection device |
| DE19523049A1 (de) * | 1995-06-24 | 1997-01-02 | Boehringer Mannheim Gmbh | Mehrschichtiges Analysenelement zur Bestimmung eines Analyten in einer Flüssigkeit |
| AUPN661995A0 (en) * | 1995-11-16 | 1995-12-07 | Memtec America Corporation | Electrochemical cell 2 |
| US6174420B1 (en) | 1996-11-15 | 2001-01-16 | Usf Filtration And Separations Group, Inc. | Electrochemical cell |
| US5962215A (en) * | 1996-04-05 | 1999-10-05 | Mercury Diagnostics, Inc. | Methods for testing the concentration of an analyte in a body fluid |
| DE19629656A1 (de) | 1996-07-23 | 1998-01-29 | Boehringer Mannheim Gmbh | Diagnostischer Testträger mit mehrschichtigem Testfeld und Verfahren zur Bestimmung von Analyt mit dessen Hilfe |
| DE19629657A1 (de) | 1996-07-23 | 1998-01-29 | Boehringer Mannheim Gmbh | Volumenunabhängiger diagnostischer Testträger und Verfahren zur Bestimmung von Analyt mit dessen Hilfe |
| DE19629655A1 (de) | 1996-07-23 | 1998-01-29 | Boehringer Mannheim Gmbh | Diagnostischer Testträger und Verfahren zur Bestimmung eines Analyts mit dessen Hilfe |
| DE19629654A1 (de) | 1996-07-23 | 1998-05-07 | Boehringer Mannheim Gmbh | Diagnostischer Testträger mit Kapillarspalt |
| US6194220B1 (en) * | 1996-09-25 | 2001-02-27 | Becton, Dickinson And Company | Non-instrumented assay with quantitative and qualitative results |
| US5714123A (en) * | 1996-09-30 | 1998-02-03 | Lifescan, Inc. | Protective shield for a blood glucose strip |
| US6379929B1 (en) | 1996-11-20 | 2002-04-30 | The Regents Of The University Of Michigan | Chip-based isothermal amplification devices and methods |
| JP3739537B2 (ja) * | 1997-03-26 | 2006-01-25 | 大日本印刷株式会社 | 光学的分析装置用測定チップ |
| US5759364A (en) * | 1997-05-02 | 1998-06-02 | Bayer Corporation | Electrochemical biosensor |
| JP3498201B2 (ja) * | 1997-08-27 | 2004-02-16 | アークレイ株式会社 | 引圧発生装置およびそれを用いた検体分析装置 |
| DE19753851A1 (de) * | 1997-12-04 | 1999-06-10 | Roche Diagnostics Gmbh | Vorrichtung zum kapillaren Flüssigkeitstransport |
| DE19753847A1 (de) * | 1997-12-04 | 1999-06-10 | Roche Diagnostics Gmbh | Analytisches Testelement mit Kapillarkanal |
| DE19753849A1 (de) | 1997-12-04 | 1999-06-10 | Roche Diagnostics Gmbh | Analytisches Testelement mit sich verjüngendem Kapillarkanal |
| DE19753850A1 (de) * | 1997-12-04 | 1999-06-10 | Roche Diagnostics Gmbh | Probennahmevorrichtung |
| US5997817A (en) * | 1997-12-05 | 1999-12-07 | Roche Diagnostics Corporation | Electrochemical biosensor test strip |
| DE19815684A1 (de) * | 1998-04-08 | 1999-10-14 | Roche Diagnostics Gmbh | Verfahren zur Herstellung von analytischen Hilfsmitteln |
-
1997
- 1997-12-04 DE DE19753850A patent/DE19753850A1/de not_active Withdrawn
-
1998
- 1998-12-03 EP EP98965748A patent/EP1035920B1/de not_active Expired - Lifetime
- 1998-12-03 US US09/554,793 patent/US7238534B1/en not_active Expired - Fee Related
- 1998-12-03 ES ES98965748T patent/ES2180229T3/es not_active Expired - Lifetime
- 1998-12-03 AU AU21576/99A patent/AU742823B2/en not_active Ceased
- 1998-12-03 CA CA002310762A patent/CA2310762C/en not_active Expired - Lifetime
- 1998-12-03 WO PCT/EP1998/007854 patent/WO1999029428A1/de not_active Application Discontinuation
- 1998-12-03 PL PL98340913A patent/PL340913A1/xx unknown
- 1998-12-03 CN CN98813487A patent/CN1115198C/zh not_active Expired - Lifetime
- 1998-12-03 JP JP2000524077A patent/JP3418173B2/ja not_active Expired - Lifetime
- 1998-12-03 AT AT98965748T patent/ATE220953T1/de not_active IP Right Cessation
- 1998-12-03 HU HU0004513A patent/HUP0004513A3/hu unknown
- 1998-12-03 DE DE59804930T patent/DE59804930D1/de not_active Expired - Lifetime
- 1998-12-03 KR KR1020007006012A patent/KR20010032723A/ko not_active Application Discontinuation
- 1998-12-04 TW TW087120207A patent/TW396273B/zh not_active IP Right Cessation
-
2001
- 2001-09-19 HK HK01106620A patent/HK1035875A1/xx not_active IP Right Cessation
-
2007
- 2007-05-29 US US11/807,548 patent/US7799578B2/en not_active Expired - Fee Related
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0010456A1 (en) * | 1978-10-25 | 1980-04-30 | EASTMAN KODAK COMPANY (a New Jersey corporation) | Liquid transport device |
Also Published As
| Publication number | Publication date |
|---|---|
| CA2310762C (en) | 2007-02-13 |
| US7799578B2 (en) | 2010-09-21 |
| DE19753850A1 (de) | 1999-06-10 |
| EP1035920B1 (de) | 2002-07-24 |
| JP2001525553A (ja) | 2001-12-11 |
| US20070266803A1 (en) | 2007-11-22 |
| PL340913A1 (en) | 2001-03-12 |
| KR20010032723A (ko) | 2001-04-25 |
| AU742823B2 (en) | 2002-01-10 |
| ES2180229T3 (es) | 2003-02-01 |
| DE59804930D1 (de) | 2002-08-29 |
| JP3418173B2 (ja) | 2003-06-16 |
| HK1035875A1 (en) | 2001-12-14 |
| EP1035920A1 (de) | 2000-09-20 |
| CN1290195A (zh) | 2001-04-04 |
| TW396273B (en) | 2000-07-01 |
| CA2310762A1 (en) | 1999-06-17 |
| US7238534B1 (en) | 2007-07-03 |
| ATE220953T1 (de) | 2002-08-15 |
| AU2157699A (en) | 1999-06-28 |
| WO1999029428A1 (de) | 1999-06-17 |
| HUP0004513A2 (hu) | 2001-04-28 |
| HUP0004513A3 (en) | 2001-05-28 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN1115198C (zh) | 在载体和覆盖层之间具有中间层的毛细管活性测试元件 | |
| US7008799B1 (en) | Analytical test element with a capillary channel | |
| US6207000B1 (en) | Process for the production of analytical devices | |
| US7043821B2 (en) | Method of preventing short sampling of a capillary or wicking fill device | |
| US6592815B1 (en) | Analytical test element with a narrowed capillary channel | |
| CN1112972C (zh) | 毛细管传输液体的装置 | |
| AU2001249468A1 (en) | Method of preventing short sampling of a capillary or wicking fill device | |
| CZ20002015A3 (cs) | Kapilárně aktivní testovací prvek opatřený mezivrstvou umístěnou mezi podkladem a krytem a jeho způsob vložení | |
| MXPA00005419A (en) | Capillary active test element having an intermediate layer situated between the support and the covering | |
| CZ20002021A3 (cs) | Analytický testovací prvek s kapilárním kanálkem | |
| MXPA00005418A (en) | Device for the capillary transport of liquid | |
| MXPA00005416A (en) | Analytic test element with a capillary canal | |
| CZ20002016A3 (cs) | Zařízení pro kapilární přenos kapaliny | |
| MXPA99003180A (en) | Process for the production of analiti devices |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CX01 | Expiry of patent term |
Granted publication date: 20030723 |
|
| CX01 | Expiry of patent term |