CN111500447A - Cell culture device for immune cell amplification - Google Patents

Cell culture device for immune cell amplification Download PDF

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Publication number
CN111500447A
CN111500447A CN202010338507.3A CN202010338507A CN111500447A CN 111500447 A CN111500447 A CN 111500447A CN 202010338507 A CN202010338507 A CN 202010338507A CN 111500447 A CN111500447 A CN 111500447A
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CN
China
Prior art keywords
electric telescopic
telescopic rod
rod
support
straw
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Granted
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CN202010338507.3A
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Chinese (zh)
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CN111500447B (en
Inventor
湛振键
齐国光
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Guangdong Kangdun Innovation Industry Group Co ltd
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Haikou Health Island Biotechnology Co ltd
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Priority to CN202010338507.3A priority Critical patent/CN111500447B/en
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00Constructional details, e.g. recesses, hinges
    • C12M23/02Form or structure of the vessel
    • C12M23/08Flask, bottle or test tube
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00Constructional details, e.g. recesses, hinges
    • C12M23/02Form or structure of the vessel
    • C12M23/04Flat or tray type, drawers
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00Constructional details, e.g. recesses, hinges
    • C12M23/02Form or structure of the vessel
    • C12M23/10Petri dish
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M33/00Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
    • C12M33/04Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles

Abstract

The invention discloses a cell culture device for immune cell amplification, which comprises a test tube rack, a plurality of culture dishes for placing different nutrient substances, a second electric telescopic rod and a plurality of first electric telescopic rods, wherein the test tube rack is used for placing test tubes, the second electric telescopic rod is arranged between the first electric telescopic rods, the top of each first electric telescopic rod is provided with an angle adjusting mechanism, the telescopic end of each first electric telescopic rod is provided with an infrared emitter, the periphery of a first through hole on the test tube rack is provided with an infrared receiver, the angle adjusting mechanism is provided with a controller, the fixed end of each first electric telescopic rod is provided with a suction tube supply mechanism, the telescopic end of each first electric telescopic rod is provided with a fixed block, the top of each fixed block is provided with a push rod for pushing the suction tube supply mechanism downwards, the bottom of each culture dish is provided with a support which is abutted against the culture dish, and the bottom, the device can automatically select and culture samples, and is suitable for test tube racks with various sizes, shapes and test tube quantity.

Description

Cell culture device for immune cell amplification
Technical Field
The invention relates to cell culture equipment, in particular to a cell culture device for immune cell amplification.
Background
Current cell culture device is when carrying out the extraction of female parent, need carry out manual extraction, to when large batch cell sample needs to be cultivateed, operating personnel need take test tube one by one and extract, and to being in the cell in different growth periods, need place the cell in the culture solution that comparatively is fit for cell growth, thereby can improve the survival rate of cell, but when selecting required cultivation sample, because the sample size is too big, artifical the time overlength of selecting, the inefficiency, moreover, to different test tube quantity, the test-tube rack of shape and size, the different test-tube rack of adaptation can not be realized to current extraction mechanism, often need to customize the sampling equipment of looks adaptation according to the size of test-tube rack and test tube quantity.
Disclosure of Invention
The invention aims to solve the problem of a cell culture device for immune cell amplification, which can automatically select culture samples, is suitable for test tube racks with various sizes, shapes and test tube numbers, and can automatically select corresponding cell samples to adapt to culture dishes with different nutrient contents.
The invention relates to a cell culture device for immune cell amplification, which comprises a test tube rack for dividing immune cell samples into different areas, a plurality of culture dishes for placing different nutrient substances, a second electric telescopic rod and a plurality of first electric telescopic rods, wherein the test tube rack is used for placing test tubes, the second electric telescopic rod is arranged between the first electric telescopic rods, the top of each first electric telescopic rod is provided with an angle adjusting mechanism, the telescopic end of each first electric telescopic rod is provided with an infrared emitter, the periphery of a first through hole on the test tube rack is provided with an infrared receiver, the angle adjusting mechanism is provided with a controller, the fixed end of each first electric telescopic rod is provided with a suction tube supply mechanism, the telescopic end of each first electric telescopic rod is provided with a fixed block, the bottom of each fixed block is provided with a push rod for pushing the suction tube supply mechanism downwards, the bottom of the culture dish is provided with a support which is abutted against the culture dish, the bottom of the support is provided with a first motor which is fixedly connected with the support, the bottom of the first motor is provided with a base, the base is provided with a third electric telescopic rod, the telescopic end of the third electric telescopic rod is provided with a connecting rod, the connecting rod is provided with a cover plate which is used for covering the support, the cover plate is provided with a communicating pipe, the culture dish is communicated with the straw supply mechanism, the telescopic end of the first electric telescopic rod is also provided with an adsorption mechanism which is used for adsorbing the support plate, the first motor, the first electric telescopic rod, the second electric telescopic rod, the third electric telescopic rod, the angle adjusting mechanism, the infrared receiver, the infrared emitter, the straw supply mechanism and the controller are all electrically connected with an external power supply, and the infrared receiver is in signal connection with, the controller is respectively in signal connection with the first motor, the first electric telescopic rod, the second electric telescopic rod, the third electric telescopic rod, the angle adjusting mechanism and the straw supply mechanism.
Further, angle adjustment mechanism includes fixed plate, second motor and slider, the bottom of fixed plate with first electric telescopic handle's top fixed connection, the output of second motor with slider fixed connection, the slider top still is equipped with the guide rail, the slider along the guide rail slides, the controller is located the top of fixed plate, the second motor with controller signal connection.
Further, the straw feeding mechanism comprises a fourth electric telescopic rod, a fixed rod and a fixed seat, the fourth electric telescopic rod is connected with the fixed seat, the fixed end of the fourth electric telescopic rod is connected with the fixed rod, the fixed end of the first electric telescopic rod is connected with the fixed rod, a plurality of first through holes are arranged on the surface of the fixed seat, the straws in interference fit with the first through holes are arranged in the first through holes, a baffle fixedly connected with the top of each straw is arranged at the top of each straw, a clamping groove is arranged at the top of each baffle and in interference fit with the push rod, a second through hole is arranged on the side wall of each straw, a plurality of channels are arranged inside the fixed seat, a plurality of hoses are arranged on the surface of the fixed seat and communicated with the channels, a negative pressure pump is arranged on each hose, and the other end of each hose is communicated, the controller is in signal connection with the fourth electric telescopic rod.
Further, adsorption apparatus constructs including supporting seat and a plurality of branch, the supporting seat with first electric telescopic handle's flexible end fixed connection, branch evenly distributed is in the bottom of supporting seat, the supporting seat with branch fixed connection.
Furthermore, the bottom of the supporting rod is provided with a sucker.
Furthermore, a vertical rod is arranged on the base, a connecting plate is arranged at the top of the vertical rod, the hose penetrates through the connecting plate, and the side face of the vertical rod is abutted to the side face of the support.
The invention has the beneficial effects that:
the invention relates to a cell culture device for immune cell expansion, wherein a plurality of test tubes for extracting cell samples are placed on a test tube rack for dividing the immune cell samples into different areas, when the cell samples are required to be collected, the controller controls the extension of the telescopic end of a first electric telescopic rod, so that a push rod can continuously move downwards, the push rod further pushes a suction tube in a suction tube supply mechanism until the suction tube enters the test tubes, the cell samples in the test tubes are sucked and conveyed into a culture dish for culture, when another cell sample with a long term is required to be cultured according to actual requirements, the direction of another first electric telescopic rod is adjusted through an angle adjusting mechanism, and then the length of the walking route of another first electric telescopic rod is adjusted through the extension or shortening of a second electric telescopic rod, when the infrared emitter on the other first electric telescopic rod is aligned with the infrared receiver on the periphery of the test tube to be detected, the infrared receiver transmits a signal to the controller, the controller controls the angle adjusting mechanism and the second electric telescopic rod to stop working, and then the controller controls the extension end of the other first electric telescopic rod to extend, so that the push rod can continuously move downwards, the push rod further pushes the suction tube in the suction tube supply mechanism until the suction tube enters the test tube filled with another cell sample of a growing period, and then the cell sample in the test tube to be cultured is sucked and conveyed into the corresponding culture dish for culturing, and then the third electric telescopic rod is shortened, so that the cover plate on the connecting rod covers the support, then pass through communicating pipe lets in the sterile air of suitable temperature to can do benefit to the cell and be in the culture dish grows, through alternate motion between the electric telescopic handle for this device can carry out the walking of multiple route according to actual need, and can also be applicable to the test-tube rack of size, shape and test tube quantity of multiple difference, can avoid appearing the mistake simultaneously and select the mistake of putting into that the cultivation sample leads to in the culture dish and lead to the condition emergence that the effect of culturing is not good, and simple structure can also select the cell culture sample automatically simultaneously, can highly link up and draw at the in-process that the cell sample drawed, and work efficiency is high.
Drawings
In order to more clearly illustrate the technical solutions in the embodiments of the present invention, the drawings needed to be used in the description of the embodiments will be briefly introduced below, and it is obvious that the drawings in the following description are only preferred embodiments of the present invention, and it is obvious for those skilled in the art that other drawings can be obtained according to these drawings without inventive exercise.
FIG. 1 is a front view of a partial structure of a cell culture apparatus for immune cell expansion according to the present invention;
FIG. 2 is a front view of another partial structure of a cell culture apparatus for immune cell expansion according to the present invention;
FIG. 3 is a schematic diagram of the combination of a test tube rack and a test tube of a cell culture apparatus for immune cell expansion according to the present invention;
FIG. 4 is a front sectional view of a holder of a cell culture apparatus for immune cell expansion according to the present invention.
In the figure, 1 is a test tube rack, 2 is a culture dish, 3 is a first electric telescopic rod, 4 is a second electric telescopic rod, 5 is a test tube, 6 is an infrared emitter, 7 is an infrared receiver, 8 is a controller, 9 is a connecting plate, 10 is a fixing block, 11 is a push rod, 12 is a support, 13 is a first motor, 14 is a base, 15 is a third electric telescopic rod, 16 is a connecting rod, 17 is a cover plate, 18 is a communicating pipe, 19 is a fixing plate, 20 is a second motor, 21 is a sliding block, 22 is a guide rail, 23 is a fourth electric telescopic rod, 24 is a fixing rod, 25 is a fixing seat, 26 is a first through hole, 27 is a suction pipe, 28 is a baffle, 29 is a clamping groove, 30 is a second through hole, 31 is a channel, 32 is a negative pressure pump, 33 is a supporting seat, 34 is a supporting rod, 35 is a suction cup, 36 is a vertical rod, and.
Detailed Description
In order to better understand the technical content of the invention, specific embodiments are provided below, and the invention is further described with reference to the accompanying drawings.
Referring to fig. 1 to 4, the cell culture device for immune cell expansion of the present invention comprises a test tube rack 1 for dividing immune cell samples into different regions, a plurality of culture dishes 2 for placing different nutrients, a second electric telescopic rod 4 and a plurality of first electric telescopic rods 3, wherein the test tube rack 1 is used for placing test tubes 5, the second electric telescopic rod 4 is arranged between the first electric telescopic rods 3, an angle adjusting mechanism is arranged on the top of the first electric telescopic rod 3, an infrared emitter 6 is arranged at the telescopic end of the first electric telescopic rod 3, an infrared receiver 7 is arranged on the periphery of the first through hole on the test tube rack 1, a controller 8 is arranged on the angle adjusting mechanism, a straw feeding mechanism is arranged at the fixed end of the first electric telescopic rod 3, a fixed block 10 is arranged at the telescopic end of the first electric telescopic rod 3, the bottom of the fixing block 10 is provided with a push rod 11 for pushing the straw feeding mechanism downwards, the bottom of the culture dish 2 is provided with a support 12 which is abutted against the support 12, the support 12 is circular in the overlooking direction, a groove (not shown) can be arranged on the support 12 for containing the culture dish 2, the bottom of the support 12 is provided with a first motor 13 which is fixedly connected with the support 12, the bottom of the first motor 13 is provided with a base 14, the base 14 is provided with a third electric telescopic rod 15, the telescopic end of the third electric telescopic rod 15 is provided with a connecting rod 16, the connecting rod 16 is provided with a cover plate 17 for covering the support 12, the cover plate 17 is provided with a communicating pipe 18, the culture dish 2 is communicated with the straw feeding mechanism, the telescopic end of the first electric telescopic rod 3 is further provided with an adsorption mechanism for adsorbing the support plate, the first motor 13, the first electric telescopic rod 3, the second electric telescopic rod 4, the third electric telescopic rod 15, the angle adjusting mechanism, the infrared receiver 7, the infrared emitter 6, the straw feeding mechanism and the controller 8 are all electrically connected with an external power supply, the infrared receiver 7 is in signal connection with the controller 8, the controller 8 is in signal connection with the first motor 13, the first electric telescopic rod 3, the second electric telescopic rod 4, the third electric telescopic rod 15, the angle adjusting mechanism and the straw feeding mechanism respectively, a plurality of test tubes 5 for extracting cell samples are placed on the test tube rack 1 for dividing the immune cell samples into different areas, when the cell samples are required to be collected, the controller 8 controls the extension of the telescopic end of the first electric telescopic rod 3, so that the push rod 11 can continuously move downwards, further, the push rod 11 pushes the pipette 27 in the pipette feeding mechanism to enter the test tube 5, so as to suck and convey the cell sample in the test tube 5 into the culture dish 2 for culture, when another cell sample with a growth period needs to be cultured according to actual needs, the direction of the other first electric telescopic rod 3 is adjusted by the angle adjusting mechanism, then the length of the traveling route of the other first electric telescopic rod 3 is adjusted by the extension or shortening of the second electric telescopic rod 4, when the infrared emitter 6 on the other first electric telescopic rod 3 is aligned with the infrared receiver 7 on the periphery of the test tube 5 to be detected, the infrared receiver 7 transmits a signal to the controller 8, and the controller 8 controls the angle adjusting mechanism and the second electric telescopic rod 4 to stop working, then the controller 8 controls the extension of the telescopic end of another first electric telescopic rod 3 to make the push rod 11 move downwards continuously, so that the push rod 11 pushes the suction tube 27 in the suction tube supply mechanism until the suction tube enters the test tube 5 filled with another cell sample of a growing period, so as to suck and convey the cell sample in the test tube 5 to be cultured into the corresponding culture dish 2 for culture, then the cover plate 17 on the connecting rod 16 covers the support 12 through the shortening of the third electric telescopic rod 15, and then sterile air with a proper temperature is introduced through the communicating tube 18, so that the growth of cells in the culture dish 2 can be facilitated, and the device can walk in various routes according to actual needs through the alternate motion between the first electric telescopic rods 3, but also can be applicable to the test-tube rack 1 of 5 quantity of size, shape and test tube of multiple difference, for example overlook the state down be circular, trapezoidal, rectangle or square test-tube rack 1, can avoid appearing the mistake simultaneously and select the mistake of putting into that cultivates the sample and lead to cultivateing the not good condition of effect and taking place in the culture dish 2, simple structure can also select the cell culture sample automatically simultaneously, can highly link up and draw at the in-process that the cell sample drawed, and work efficiency is high.
Specifically, the angle adjusting mechanism includes a fixed plate 19, a second motor 20 and a sliding block 21, the bottom of the fixed plate 19 is fixedly connected to the top of the first electric telescopic rod 3, the output end of the second motor 20 is fixedly connected to the sliding block 21, a guide rail 22 is further disposed above the sliding block 21, the sliding block 21 slides along the guide rail 22, the controller 8 is disposed at the top of the fixed plate 19, the second motor 20 is in signal connection with the controller 8, the controller 8 controls the rotation of the second motor 20, so that the sliding block 21 rotates under the driving action of the second motor 20, and further the adjustment of the orientation angle of the other first electric telescopic rod 3 is realized, and through the relative movement between the sliding block 21 and the guide rail 22, the relative movement between the sliding block 21 and the guide rail 22 can be realized according to the extension or shortening of the second electric telescopic rod 4, through angle adjustment mechanism's effect can move towards the position of the cell sample that needs to draw according to actual need to realize automatic quick acquisition the function of the cell sample in the test tube 5.
Specifically, the straw feeding mechanism comprises a fourth electric telescopic rod 23, a fixing rod 24 and a fixing seat 25, the fourth electric telescopic rod 23 is connected with the fixing seat 25, the fixing end of the fourth electric telescopic rod 23 is connected with the fixing rod 24, the fixing end of the first electric telescopic rod 3 is connected with the fixing rod 24, a plurality of first through holes 26 are arranged on the surface of the fixing seat 25, the straw 27 in interference fit with the first through holes 26 is arranged in the first through holes 26, a baffle 28 fixedly connected with the straw 27 is arranged at the top of the straw 27, a clamping groove 29 is arranged at the top of the baffle 28, the clamping groove 29 is in interference fit with the push rod 11, a second through hole 30 is arranged on the side wall of the straw 27, a plurality of channels 31 are arranged inside the fixing seat 25, a plurality of hoses 37 are arranged on the surface of the fixing seat 25, and the hoses 37 can, the liquid transportation failure caused by the tube orifice being compressed can be reduced, the flexible tube 37 is communicated with the channel 31, the flexible tube 37 is provided with the negative pressure pump 32, the other end of the flexible tube 37 is communicated with the culture dish 2, the controller 8 is in signal connection with the fourth electric telescopic rod 23, when the first suction tube 27 in the fixed seat 25 on one of the first electric telescopic rods 3 is used, in order to avoid the cross contamination between cell samples and collect the next cell sample in the test tube 5, the other first electric telescopic rod 3 is moved to the detection position, the switching is realized through the alternate movement of the first electric telescopic rod 3, and the first electric telescopic rod 3 which is realizing the moving work is contracted during the alternate movement of the first electric telescopic rod 3, so that the push rod 11 of the first electric telescopic rod 3 is drawn out from the first through hole 26, the suction pipe 27 can be partially received into the first through hole 26 due to the friction force generated by the interference fit between the locking groove 29 and the push rod 11, then the fixing seat 25 is moved by the extension of the fourth electric telescopic rod 23, so that the next first through hole 26 is aligned with the push rod 11, then the push rod 11 is moved downwards by the extension of the first electric telescopic rod 3 until being inserted into the first through hole 26, so that the second suction pipe 27 is pushed out, when the first electric telescopic rod 3 is extended to the limit, the second through hole 30 of the suction pipe 27 is aligned with the channel 31, so that the cell sample in the test tube 5 is sucked out by the action of the negative pressure pump 32 and is transferred to the culture dish 2 through the hose 37 for culture, by using the suction pipe supply mechanism, the suction pipe 27 does not need to be frequently replaced when the device is applied to large-scale cell sample extraction, the replacement of the suction pipe 27 is realized when the first electric telescopic rod 3 is switched to the position, and the detection efficiency is effectively improved.
Specifically, adsorption apparatus constructs including supporting seat 33 and a plurality of branch 34, supporting seat 33 with the flexible end fixed connection of first electric telescopic handle 3, branch 34 evenly distributed is in the bottom of supporting seat 33, supporting seat 33 with branch 34 fixed connection, through the cooperation of supporting seat 33 and branch 34, can be with more stable the fixing of this device the top of backup pad.
Specifically, the bottom of the supporting rod 34 is provided with a suction cup 35, so that the stability of the device on the supporting plate can be improved.
Specifically, be equipped with montant 36 on the base 14, the top of montant 36 is equipped with connecting plate 9, hose 37 passes connecting plate 9, the side of montant 36 with the side looks butt of support 12 can with hose 37 is fixed, makes things convenient for this device to use.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents, improvements and the like that fall within the spirit and principle of the present invention are intended to be included therein.

Claims (6)

1. A cell culture device for immune cell amplification is characterized by comprising a test tube rack for dividing immune cell samples into different areas, a plurality of culture dishes for placing different nutrient substances, a second electric telescopic rod and a plurality of first electric telescopic rods, wherein the test tube rack is used for placing test tubes, the second electric telescopic rod is arranged between the first electric telescopic rods, an angle adjusting mechanism is arranged at the top of each first electric telescopic rod, an infrared emitter is arranged at the telescopic end of each first electric telescopic rod, an infrared receiver is arranged at the periphery of each first through hole on the test tube rack, a controller is arranged on each angle adjusting mechanism, a straw supply mechanism is arranged at the fixed end of each first electric telescopic rod, a fixed block is arranged at the telescopic end of each first electric telescopic rod, a push rod for pushing the straw supply mechanism downwards is arranged at the bottom of the fixed block, the bottom of the culture dish is provided with a support which is abutted against the culture dish, the bottom of the support is provided with a first motor which is fixedly connected with the support, the bottom of the first motor is provided with a base, the base is provided with a third electric telescopic rod, the telescopic end of the third electric telescopic rod is provided with a connecting rod, the connecting rod is provided with a cover plate which is used for covering the support, the cover plate is provided with a communicating pipe, the culture dish is communicated with the straw supply mechanism, the telescopic end of the first electric telescopic rod is also provided with an adsorption mechanism which is used for adsorbing the support plate, the first motor, the first electric telescopic rod, the second electric telescopic rod, the third electric telescopic rod, the angle adjusting mechanism, the infrared receiver, the infrared emitter, the straw supply mechanism and the controller are all electrically connected with an external power supply, and the infrared receiver is in signal connection with, the controller is respectively in signal connection with the first motor, the first electric telescopic rod, the second electric telescopic rod, the third electric telescopic rod, the angle adjusting mechanism and the straw supply mechanism.
2. The cell culture device for immune cell expansion of claim 1, wherein the angle adjustment mechanism comprises a fixed plate, a second motor and a slide block, the bottom of the fixed plate is fixedly connected with the top of the first electric telescopic rod, the output end of the second motor is fixedly connected with the slide block, a guide rail is further arranged above the slide block, the slide block slides along the guide rail, the controller is arranged on the top of the fixed plate, and the second motor is in signal connection with the controller.
3. The cell culture device according to claim 1, wherein the straw feeding mechanism comprises a fourth electric telescopic rod, a fixing rod and a fixing seat, the fourth electric telescopic rod is connected with the fixing seat, the fixing end of the fourth electric telescopic rod is connected with the fixing rod, the fixing end of the first electric telescopic rod is connected with the fixing rod, the surface of the fixing seat is provided with a plurality of first through holes, the straw is arranged in the first through holes in interference fit with the first through holes, the top of the straw is provided with a baffle plate fixedly connected with the straw, the top of the baffle plate is provided with a clamping groove, the clamping groove is in interference fit with the push rod, the side wall of the straw is provided with a second through hole, the inside of the fixing seat is provided with a plurality of channels, the surface of the fixing seat is provided with a plurality of hoses, and the hoses are communicated with the channels, the flexible pipe is provided with a negative pressure pump, the other end of the flexible pipe is communicated with the culture dish, and the controller is in signal connection with the fourth electric telescopic rod.
4. The device of claim 1, wherein the suction mechanism comprises a support base and a plurality of support rods, the support base is fixedly connected to the telescopic end of the first electric telescopic rod, the support rods are uniformly distributed at the bottom of the support base, and the support base is fixedly connected to the support rods.
5. The cell culture apparatus for immune cell expansion according to claim 1, wherein the bottom of the support rod is provided with a suction cup.
6. The cell culture device for immune cell expansion of claim 1, wherein the base is provided with a vertical rod, the top of the vertical rod is provided with a connecting plate, the hose passes through the connecting plate, and the side surface of the vertical rod abuts against the side surface of the support.
CN202010338507.3A 2020-04-26 2020-04-26 Cell culture device for immune cell expansion Active CN111500447B (en)

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