CN104401922A - Automatic dumping device for hyperbaric chamber cell culture reagent - Google Patents
Automatic dumping device for hyperbaric chamber cell culture reagent Download PDFInfo
- Publication number
- CN104401922A CN104401922A CN201410472319.4A CN201410472319A CN104401922A CN 104401922 A CN104401922 A CN 104401922A CN 201410472319 A CN201410472319 A CN 201410472319A CN 104401922 A CN104401922 A CN 104401922A
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- CN
- China
- Prior art keywords
- liquid storage
- test tube
- hyperbaric chamber
- cell culture
- cabin
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- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Classifications
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B67—OPENING, CLOSING OR CLEANING BOTTLES, JARS OR SIMILAR CONTAINERS; LIQUID HANDLING
- B67C—CLEANING, FILLING WITH LIQUIDS OR SEMILIQUIDS, OR EMPTYING, OF BOTTLES, JARS, CANS, CASKS, BARRELS, OR SIMILAR CONTAINERS, NOT OTHERWISE PROVIDED FOR; FUNNELS
- B67C9/00—Devices for emptying bottles, not otherwise provided for
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention discloses an automatic dumping device for hyperbaric chamber cell culture reagents. A chamber door of the hyperbaric chamber is arranged at the top; the side of the chamber body is provided with an electrical penetration piece; an electric control system includes a drive mechanism positioned outside the chamber and a two-phase hybrid stepping motor located in the chamber, and is used to connect the signal lines outside the chamber to a signal terminal of the motor through the electrical penetration piece; a transmission mechanism connector connects the motor shaft of the two-phase hybrid stepping motor with a liquid storage mechanism, the connector drives the liquid storage mechanism to rotate for the same angle, so as to successfully pour reagents in one group of test tubes into the cell culture dish; a liquid storage rack is provided with a plurality of test tube place positions, two connected groups of liquid storage rack have a certain angle, and place positions of different groups of test tubes are separated. The device provided by the invention has reasonable structure, can operate normally in the hyperbaric chamber automatically dump cell culture reagents, and add a variety of reagents at the same time or add different reagents at different time, so as to improve the experimental efficiency.
Description
Technical field
The present invention relates to a kind of hyperbaric chamber cell culture reagent automatic tipping device.
Background technology
In order to solve the nitrogen narcosis problem that the deep ocean work mankind occur, the researchist in nautical medicine field will carry out the experiment of neuronal cell cultivation in hyperbaric chamber.Medical science hyperbaric chamber can simulate the pressure environment at deep-sea, neuronal cell is placed in the culture dish in cabin, in experimentation, needs timing to add different exogenous agents (activator, inhibitor etc.) to observe and to detect the function of receptors of neuronal cell under high pressure conditions.For guaranteeing the accuracy that the state of neuronal cell does not change and tests, the interpolation of exogenous agents must complete under airtight high pressure conditions, and only having experiment to complete disposable decompression could open hatch door.At present, this supporting operatorless device, does not add reagent and all manually completes afterwards in decompression in experimentation, and some uncertain factors thus cannot be avoided the impact of experiment.
Summary of the invention
The object of the present invention is to provide a kind of rational in infrastructure, normally can run in hyperbaric chamber also Automatic-dumping cell culture reagent, plurality of reagents can be added simultaneously also can not add the hyperbaric chamber cell culture reagent automatic tipping device of different reagent in the same time.
Technical solution of the present invention is:
A kind of hyperbaric chamber cell culture reagent automatic tipping device, is characterized in that: comprise hyperbaric chamber, electric control system, transmission device, liquid storage mechanism; The hatch door of hyperbaric chamber is located at top, and cabin body side surface arranges electrical penetration, for the equipment outside connecting cabin and the actuating unit in cabin; Electric control system comprises the driver train be positioned at out of my cabin and the two-phase hybrid stepping motor being positioned at cabin, is connected on the signal end of motor by electrical penetration by signal wire (SW) out of my cabin; The motor shaft of two-phase hybrid stepping motor and liquid storage mechanism couple together by transmission device adaptor union, and utilize nut and nut to lock, when stepping motor rotates according to set angle, adaptor union drives liquid storage mechanism to rotate identical angle, thus is successfully poured in Tissue Culture Dish by the reagent in wherein one group of test tube; The physical construction that described adaptor union has " one is even many ", " one " refers to a motor shaft, and " many " refer to multiple liquid storage frame; Described liquid storage mechanism is made up of multiple liquid storage frame and test tube, liquid storage frame there is multiple test tube place position, the two groups of liquid storage framves that are connected are placed at an angle, and the test tube placement position of different group is in staggered distribution, and makes the reagent of different group in electric machine rotation process fully can pour culture dish into successively.
The inner side that position placed by test tube has certain thickness caoutchouc elasticity circle, and object fixes test tube; Described test tube has one section of recessed circular curved surface at close mouth of pipe place, test tube is put into placement position rear curved surface and is just in time stuck in caoutchouc elasticity circle place.
Stepping motor is fixed on by connecting element to be had on the additional base of certain altitude, the stability of whole equipment when ensureing that motor runs.
The present invention is rational in infrastructure, normally can run and Automatic-dumping cell culture reagent, can add plurality of reagents simultaneously and also can not add different reagent in the same time in hyperbaric chamber; Electric control system had not only met requirement but also convenient operation person oneself the setting operational factor of smooth operation under hyperbaric environment; The design of mechanical transmission mechanism and liquid storage mechanism makes device achieve the function of not adding different reagent in the same time.The reagent automatic tipping device of low cost is that nautical medicine experiment provides rigorous experiment condition, improves conventional efficient.
Accompanying drawing explanation
Below in conjunction with drawings and Examples, the invention will be further described.
Fig. 1 is the structural representation of one embodiment of the invention.
Wherein, 1. hyperbaric chamber body, 11. hyperbaric chamber base plates, 12. hyperbaric chamber hatch doors, 13. electrical penetrations, 21. drive power supplies, 22. actuators, 23. controllers, 24. two-phase hybrid stepping motors, 25. signal terminals, 26. motor shafts, 27. connecting elements, 28. additional base, 3. adaptor unions, 31. nuts, 32. nuts, 4. liquid storage mechanism, 41. liquid storage framves, 42. elastic rubber rings, position placed by 43. test tubes, 44. test tubes, 5. culture dish.
Fig. 2 is the structural representation that mechanical transmission mechanism and liquid storage mechanism assemble.
Fig. 3 is the perspective view that mechanical transmission mechanism and liquid storage mechanism assemble.
Fig. 4 is the structural representation of liquid storage test tube.
Fig. 5 is the process schematic of plurality of reagents Automatic-dumping.
Detailed description of the invention
As shown in Figure 1, a kind of hyperbaric chamber cell culture reagent automatic tipping device, it comprises hyperbaric chamber 1, electric control system 2, transmission device 3 and liquid storage mechanism 4.
The hatch door 12 of hyperbaric chamber 1 is located at top, is convenient to the operation such as the installation of below deck equipment, the replacing of reagent.Electrical penetration 13 Main Function of cabin body side surface is the equipment outside connecting cabin and the actuating unit in cabin.
Electric control system 2 comprises the driving power 21, actuator 22, the controller 23 that are positioned at out of my cabin further and is positioned at the actr two-phase hybrid stepping motor 24 in cabin.The good touch-screen of man-machine interaction selected by controller 23, and operating personal can sets itself operational mode and time of run.Stepping motor 24 is two-phase four-wire system, and be connected on the signal end 25 of motor by electrical penetration 13 by the signal wire (SW) of 4 out of my cabin, signal voltage 5V, meets national standard.In addition, stepping motor is fixed on by connecting element 27 to be had on the additional base 28 of certain altitude, the stability of whole equipment when ensureing that motor runs.
As shown in Figure 2, motor shaft 26 and liquid storage mechanism 4 couple together by transmission device adaptor union 3, and utilize nut 31 and nut 32 to lock, when stepping motor 24 rotates according to set angle, adaptor union 3 drives liquid storage mechanism 4 to rotate identical angle, thus is successfully poured in Tissue Culture Dish by the reagent in wherein one group of test tube 44.The physical construction that described adaptor union 3 has " one is even many ", " one " refers to a motor shaft 26, and " many " refer to multiple liquid storage frame 41.Described liquid storage mechanism is made up of multiple liquid storage frame 41 and test tube.Liquid storage frame 41 has multiple test tube place position 43, the inner side that position 43 placed by test tube has certain thickness caoutchouc elasticity circle 42, and object fixes test tube 44.The two groups of liquid storage framves 41 that are connected are placed at an angle, and the test tube placement position 43 of different group is in staggered distribution, and makes the reagent of different group in electric machine rotation process fully can pour culture dish into successively.
As shown in Figure 3, described test tube 44 and common test tube different, put into having the circular curved surface 6 of one section of nick, test tube 44 near mouth of pipe place and place position 43 rear curved surface and be just in time stuck in caoutchouc elasticity circle 42 place, so just can ensure that test tube does not come off, and is also convenient for changing reagent.
Shown in Fig. 5 is the process schematic that different time adds different reagent, the angle of toppling over, and interlude can set on controller 23.
Claims (3)
1. a hyperbaric chamber cell culture reagent automatic tipping device, is characterized in that: comprise hyperbaric chamber, electric control system, transmission device, liquid storage mechanism; The hatch door of hyperbaric chamber is located at top, and cabin body side surface arranges electrical penetration, for the equipment outside connecting cabin and the actuating unit in cabin; Electric control system comprises the driver train be positioned at out of my cabin and the two-phase hybrid stepping motor being positioned at cabin, is connected on the signal end of motor by electrical penetration by signal wire (SW) out of my cabin; The motor shaft of two-phase hybrid stepping motor and liquid storage mechanism couple together by transmission device adaptor union, and utilize nut and nut to lock, when stepping motor rotates according to set angle, adaptor union drives liquid storage mechanism to rotate identical angle, thus is successfully poured in Tissue Culture Dish by the reagent in wherein one group of test tube; The physical construction that described adaptor union has " one is even many ", " one " refers to a motor shaft, and " many " refer to multiple liquid storage frame; Described liquid storage mechanism is made up of multiple liquid storage frame and test tube, liquid storage frame there is multiple test tube place position, the two groups of liquid storage framves that are connected are placed at an angle, and the test tube placement position of different group is in staggered distribution, and makes the reagent of different group in electric machine rotation process fully can pour culture dish into successively.
2. hyperbaric chamber cell culture reagent automatic tipping device according to claim 1, is characterized in that: the inner side that position placed by test tube has certain thickness caoutchouc elasticity circle, and object fixes test tube; Described test tube has one section of recessed circular curved surface at close mouth of pipe place, test tube is put into placement position rear curved surface and is just in time stuck in caoutchouc elasticity circle place.
3. hyperbaric chamber cell culture reagent automatic tipping device according to claim 1, is characterized in that: stepping motor is fixed on by connecting element to be had on the additional base of certain altitude, the stability of whole equipment when ensureing that motor runs.
Priority Applications (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610018642.3A CN105668495B (en) | 2014-09-17 | 2014-09-17 | A kind of hyperbaric chamber cell culture reagent automatic tipping device |
| CN201610018643.8A CN105645339B (en) | 2014-09-17 | 2014-09-17 | Improve the hyperbaric chamber cell culture reagent automatic tipping device of conventional efficient |
| CN201410472319.4A CN104401922B (en) | 2014-09-17 | 2014-09-17 | Hyperbaric chamber cell culture reagent automatic tipping device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410472319.4A CN104401922B (en) | 2014-09-17 | 2014-09-17 | Hyperbaric chamber cell culture reagent automatic tipping device |
Related Child Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610018643.8A Division CN105645339B (en) | 2014-09-17 | 2014-09-17 | Improve the hyperbaric chamber cell culture reagent automatic tipping device of conventional efficient |
| CN201610018642.3A Division CN105668495B (en) | 2014-09-17 | 2014-09-17 | A kind of hyperbaric chamber cell culture reagent automatic tipping device |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN104401922A true CN104401922A (en) | 2015-03-11 |
| CN104401922B CN104401922B (en) | 2016-05-25 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410472319.4A Expired - Fee Related CN104401922B (en) | 2014-09-17 | 2014-09-17 | Hyperbaric chamber cell culture reagent automatic tipping device |
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| Country | Link |
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| CN (1) | CN104401922B (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111500447A (en) * | 2020-04-26 | 2020-08-07 | 海口健康岛生物科技有限公司 | Cell culture device for immune cell amplification |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN86204754U (en) * | 1986-11-04 | 1987-09-30 | 海军医学研究所 | Dynamic video display device of micro-circulation under high atmospheric pressure environment |
| CN101926680A (en) * | 2010-08-02 | 2010-12-29 | 曹罡 | PTCD (Percutaneous transhepatic cholangiodrainage) auxiliary machinery automatic device |
| CN101989078A (en) * | 2009-08-03 | 2011-03-23 | 天津工程师范学院 | PLC based automatic control system of robot for cleaning oil storage tank |
| CN203552043U (en) * | 2013-11-18 | 2014-04-16 | 陈达波 | Electric automated dust removal device |
-
2014
- 2014-09-17 CN CN201410472319.4A patent/CN104401922B/en not_active Expired - Fee Related
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN86204754U (en) * | 1986-11-04 | 1987-09-30 | 海军医学研究所 | Dynamic video display device of micro-circulation under high atmospheric pressure environment |
| CN101989078A (en) * | 2009-08-03 | 2011-03-23 | 天津工程师范学院 | PLC based automatic control system of robot for cleaning oil storage tank |
| CN101926680A (en) * | 2010-08-02 | 2010-12-29 | 曹罡 | PTCD (Percutaneous transhepatic cholangiodrainage) auxiliary machinery automatic device |
| CN203552043U (en) * | 2013-11-18 | 2014-04-16 | 陈达波 | Electric automated dust removal device |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111500447A (en) * | 2020-04-26 | 2020-08-07 | 海口健康岛生物科技有限公司 | Cell culture device for immune cell amplification |
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| Publication number | Publication date |
|---|---|
| CN104401922B (en) | 2016-05-25 |
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