CN109666587A - A kind of dynamic cell culture plate - Google Patents
A kind of dynamic cell culture plate Download PDFInfo
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- CN109666587A CN109666587A CN201811243800.0A CN201811243800A CN109666587A CN 109666587 A CN109666587 A CN 109666587A CN 201811243800 A CN201811243800 A CN 201811243800A CN 109666587 A CN109666587 A CN 109666587A
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M25/00—Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
- C12M25/06—Plates; Walls; Drawers; Multilayer plates
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Abstract
The present invention relates to experimental instrument fields, disclose a kind of dynamic cell culture plate, and the dynamic cell culture plate includes supporting plate, cell culture well, driving device, support base, drive shaft, gear, connecting rod, culture foundation trench, preformed hole and impeller.When use, start driving device, driving device is rotated by driving shaft driven gear and connecting rod, the impeller of connection boom end rotates with, under impeller effect, culture medium can flow in culture foundation trench, and the cell in such cell culture well is in dynamic environment, realizes cell dynamic cultivation.It is static culture plate that dynamic cell culture plate of the invention, which solves existing most cells culture plate, can not the intracorporal dynamic environment of analog machine to cell carry out dynamic cultivation the problem of, have many advantages, such as it is small in size, at low cost, easy to use, experimental result accuracy and science can be improved.
Description
Technical field
The present invention relates to experimental instrument fields, more particularly to a kind of dynamic cell culture plate.
Background technique
At present most of common tissue culture plate in laboratory be static culture plate, culture medium be it is static, cell is quiet
It is cultivated under state environment, and in vivo, tissue fluid can flow, therefore cell is moved in dynamic environment.It is static
Cell state, function for cultivating on tissue culture plate etc. are all variant with the lower culture of dynamic, and experimental result may also can be with life
The intracorporal cell detection results of object are variant.Therefore, it is necessary to which culture is thin in a dynamic environment using dynamic cell culture plate
Born of the same parents maintain cell normal function, differentiation, secretion etc., improve the accuracy and science of experimental result.
Also have a kind of instrument on the market, can to cultivate cell under flow regime, however mainly for detection of hydrodynamic shear,
It is a kind of fluid shearing Force system, disadvantage is that: 1. is not suitable for various experiments, and use scope is small；2. instrument valence
Lattice are expensive, and cell plates are also more expensive, at high cost；3. the equipment for needing to connect when use is more, inconvenient to use；4. volume
Greatly, inconvenience is put into cell incubator and uses.
In conclusion researching and developing, one kind is small in size, at low cost, easy to use, can simulate the intracorporal dynamic environment of biology to train
Cell is supported, experimental result accuracy is improved and scientific dynamic cell culture plate is actually necessary.
Summary of the invention
The present invention in order to overcome at least one of the drawbacks of the prior art described above (deficiency), provide it is a kind of it is small in size, at
This dynamic cell culture plate low, easy to use.
In order to solve the above technical problems, the technical scheme adopted by the invention is as follows:
A kind of dynamic cell culture plate, including supporting plate, cell culture well, which is characterized in that the dynamic cell culture plate also wraps
Include driving device, support base, drive shaft, gear, connecting rod, culture foundation trench, preformed hole and impeller, the driving device and branch
Support seat is fixedly connected, and the drive shaft connect by gear with connecting rod, the driving device by drive shaft driven gear and
Connecting rod rotation, the connecting rod is through culture medium groove sidewall and connection boom end is provided with impeller, the cell culture well and
Preformed hole setting is inside culture foundation trench.Dynamic cell culture plate bulk of the invention is small, at low cost, easy to use, simulation life
Dynamic environment culture cell in object, can be improved experimental result accuracy and science.
Further, the cell culture well sidewall symmetry of the dynamic cell culture plate is provided with through-hole, each cell training
It supports a mating dismountable circular sleeve, the height of sleeve inside hole and is slightly above cell culture well, be convenient for from cell culture well
Interior taking-up.The cell culture well is suitble to cultivate attached cell, tests initial stage, sleeve is first put into cell culture well, thin in this way
The through-hole of born of the same parents' culture hole side wall is blocked, and the culture medium in cell culture well is in closing stationary state, and cell can be trained in cell
Support hole internal backplane on carry out it is adherent, after cell completely it is adherent after, take away sleeve, culture medium and training in such cell culture well
Culture medium in feeding foundation trench, which can converge, to be integrated.
Further, the culture foundation trench of the dynamic cell culture plate, which is square, is symmetricly set on supporting plate, is distributed in drive
Dynamic two sides, the driving device are set on support base, and the support base is set on supporting plate, drive shaft one end connection
Driving device, the other end of drive shaft are flexibly connected with the support rod being arranged on supporting plate, and drive shaft surface is equidistantly positioned outer
Screw thread, the external screw thread are engaged with the gear being fixedly installed in the middle part of connecting rod, and the both ends of the connecting rod extend through corresponding
Culture medium groove sidewall and end be provided with impeller.Culture foundation trench is square distribution, is adapted to more experimental occasions, simultaneously
The quantity of culture foundation trench experimental can need to increase or decrease in even numbers.Using gear transmission mode, both may be implemented
The deflecting of driving force, while having the characteristics that stable transmission, high-efficient, compact-sized again.
Further, one cell culture well of setting and a preformed hole in the culture foundation trench of the distribution that is square.Carefully
Born of the same parents' culture hole is used to cultivate attached cell, and setting preformed hole both can use its contour structures and form culture medium in culture foundation trench
Special flow passage, can also in preformed hole static culture cell, or the controlled observation area as cell culture.
Further, two cell culture wells of setting and a preformed hole in the culture foundation trench of the distribution that is square.If
The purpose for setting two cell culture wells is each a kind of cell of cell culture well culture for cultivating two kinds of cells, such two kinds
Cell life can analyze the interaction and influence between two kinds of cells of observation in the culture medium of same flowing.Setting is pre-
Boxing out both can use the special flow passage that its contour structures forms culture medium in culture foundation trench, can also be static in preformed hole
Cultivate cell, or the controlled observation area as cell culture.
Further, the culture foundation trench of the dynamic cell culture plate is rounded is arranged on supporting plate, the driving device
It is set to inside support base, the support base is set to above supporting plate by support column, and support base is run through in described drive shaft one end
Bottom connects driving device, and first bevel gear is arranged in the other end of drive shaft, and second bevel gear is arranged in one end of the connecting rod
And engaged with the first bevel gear of driveshaft end setting, the other end of connecting rod runs through corresponding culture medium groove sidewall and end
It is provided with impeller, has a cell culture well and a preformed hole in the culture foundation trench.The rounded distribution of foundation trench is cultivated, it can be with
More experimental occasions are adapted to, while the quantity for cultivating foundation trench experimental can need to increase or decrease, and need to only increase simultaneously
The quantity for the connecting rod with bevel gear that adds deduct less.Using gear transmission mode, the deflecting of driving force both may be implemented,
Has the characteristics that stable transmission, high-efficient, compact-sized again simultaneously.
Further, the connecting rod of the dynamic cell culture plate is equipped with rubber seal through the position of culture medium groove sidewall
Circle.In this way in connecting rod rotation process, the abrasion of culture medium groove sidewall Yu connecting rod junction can be both reduced, elimination is made an uproar
Sound, and sealing function can be played, prevent culture medium from leaking into outside, while also can be used as a fulcrum of connecting rod, it is ensured that
The dynamic balancing of connecting rod.
Further, the impeller of the dynamic cell culture plate can choose unshrouded impeller, semi-open type or double shrouded wheel,
The preferred double shrouded wheel of the present invention, impeller are made of forward and backward cover board and arc shaped blade, and arc shaped blade is fixed at forward and backward cover board
Between.Double shrouded wheel efficiency is higher, while can prevent culture solution from splashing around, it is ensured that culture medium is steady under impeller effect
Flowing.
Further, the culture foundation trench of the dynamic cell culture plate, cell culture well, preformed hole shape can do
At arbitrary shape, as long as being able to achieve technical effect of the invention, meet the requirement of dynamic cultivation cell.
Further, to prevent cell sample contaminated, the dynamic cell culture plate can a mating transparent housing,
The material of transparent housing can select the materials such as plastics, organic glass to be made, and not only convenient for observation cell culture state, but also can incite somebody to action
Cell sample is isolated from the outside, and prevents cell sample contaminated.The shape of transparent housing can be made arbitrary shape, as long as can incite somebody to action
Cell sample on the tissue culture plate is isolated just with external environment, is not repeated it in the present invention.
In some embodiments, it is described culture foundation trench be square distribution dynamic cell culture plate driving device for electricity
Machine device uses battery to provide power supply for motor, and electric machine bottom is fixedly connected with support base, the side connection of electric machine
Drive shaft, support base inside setting battery case and power supply line, power supply line connect motor and battery case, setting outside support base
Power switch.It being driven using electric machine, structure is simple, and it is small in size, it is easy to use, run lasting stability.
In some embodiments, it is described culture foundation trench be square distribution dynamic cell culture plate driving device for hair
Strip device, clockwork bottom are fixedly connected with support base, and the side of clockwork connects drive shaft, the other side of clockwork
Clockwork spring handle is set.It being driven using clockwork, is not necessarily to energy consumption, structure is simple, and it is small in size, it is easy to use.
In some embodiments, the driving device of the dynamic cell culture plate of the culture rounded distribution of foundation trench is electricity
Machine device uses battery to provide power supply for motor, and electric machine is installed on inside support base, and the drive shaft runs through support base bottom
Portion is connect with electric machine, and power switch is arranged outside the support base, is additionally provided with battery case and power supply line inside support base,
Power supply line connects motor and battery case.It is driven using electric machine, structure is simple, and small in size, easy to use, operation is persistently steady
It is fixed.
In some embodiments, the driving device of the dynamic cell culture plate of the culture rounded distribution of foundation trench is hair
Strip device, clockwork are installed on inside support base, and drive shaft is connected through support base bottom and the side of clockwork, clockwork spring
Handle is connect at the top of support base with the other side of clockwork.It being driven using clockwork, is not necessarily to energy consumption, structure is simple,
It is small in size, it is easy to use.
No matter which kind of embodiment is used, electric machine and clockwork are conventional drive device, therefore its structure is former
Reason repeats no more.
Dynamic cell culture plate disclosed by the invention when in use, starts driving device, and driving device passes through drive shaft band
The impeller of the dynamic gear rotation being set in connecting rod, connecting rod and connection boom end setting is rotated with gear, is acted in impeller
Under, culture medium can flow in culture foundation trench, and the cell in such cell hole is at dynamic environment, realize cell dynamic
Culture.
The description of " first ", " second " referred to above, is used for description purposes only, is not understood to indicate or imply
Its relative importance or the quantity for implicitly indicating indicated technical characteristic, define the feature of " first ", " second " as a result,
It can explicitly or implicitly include at least one of the features.
Compared with prior art, the beneficial effect of technical solution of the present invention is:
1. small in size: being easily put into the instruments such as cell incubator and super-clean bench and use；
2. at low cost: structure is simple, and attachment is few, easily fabricated；
3. easy to use: it is easy to operate, it is not required to connect excessive equipment and AC power source；
4. simulating dynamic environment culture cell in organism, experimental result accuracy and science can be improved.
Detailed description of the invention
To describe the technical solutions in the embodiments of the present invention more clearly, make required in being described below to embodiment
Attached drawing is briefly described, it should be apparent that, drawings in the following description are only some embodiments of the invention, for
For those of ordinary skill in the art, without creative efforts, it can also be obtained according to these attached drawings other
Attached drawing.
Fig. 1 is the schematic perspective view of embodiment 1；
Fig. 2 is the top view of embodiment 1；
Fig. 3 is the schematic perspective view of embodiment 2；
Fig. 4 is the top view of embodiment 2；
Fig. 5 is the schematic perspective view of embodiment 3；
Fig. 6 is the top view of embodiment 3；
Fig. 7 is the schematic perspective view of embodiment 4；
Fig. 8 is the top view of embodiment 4；
Fig. 9 is the schematic perspective view of embodiment 5；
Figure 10 is the schematic perspective view of embodiment 6；
Figure 11 is each member connection structure schematic diagram of embodiment 5-6；
Figure 12 is the structural schematic diagram of cell culture well and sleeve；
Figure 13 is the schematic perspective view of impeller；
Figure 14 is the A-A sectional view of Figure 13；
The drive shaft and gear connection structure schematic diagram that Figure 15 is embodiment 1-4；
Figure 16 is the first, second bevel gear connection structure side view of embodiment 5-6.
In figure: 1- supporting plate, 2- cell culture well, 3- driving device, 4- drive shaft, 5- support rod,
6- support base, 7- support column, 8- gear, 9- external screw thread, 10- connecting rod,
11- first bevel gear, 12- second bevel gear, 13- culture foundation trench, 14- impeller,
15- preformed hole, 16- sleeve, 17- through-hole, 18- rubber seal, 19- clockwork spring handle,
20- power switch.
Specific embodiment
The attached figures are only used for illustrative purposes and cannot be understood as limitating the patent；In order to which the present embodiment is better described,
The certain components of attached drawing have omission, zoom in or out, and do not represent the size of actual product；Those skilled in the art are come
It says, the omitting of some known structures and their instructions in the attached drawings are understandable.
The description of " first ", " second ", is used for description purposes only involved in the present invention, and should not be understood as instruction or
It implies its relative importance or implicitly indicates the quantity of indicated technical characteristic, define " first ", " second " as a result,
Feature can explicitly or implicitly include at least one of the features.
In the description of the present invention, it should be noted that unless otherwise clearly defined and limited, term " installation " " connects
Connect " it shall be understood in a broad sense, for example, it may be being fixedly connected, it may be a detachable connection, or be integrally connected；It can be machine
Tool connection, is also possible to be electrically connected；It can be directly connected, be also possible to be indirectly connected with by intermediary, it may be said that two
Connection inside element.For the ordinary skill in the art, above-mentioned term can be understood at this as the case may be
The concrete meaning of invention.
For a clearer understanding of the technical characteristics, objects and effects of the present invention, now control attached drawing is described in detail
A specific embodiment of the invention, embodiments of the present invention are not limited thereto.
Embodiment 1:
As shown in Figure 1, Figure 2 and shown in Figure 15, a kind of dynamic cell culture plate, including supporting plate 1, cell culture well 2, driving device 3, branch
Support seat 6, drive shaft 4, gear 8, connecting rod 10, culture foundation trench 13, preformed hole 15 and impeller 14, the driving device 3 and branch
Support seat 6 is fixedly connected, and the drive shaft 4 is connect by gear 8 with connecting rod 10, and the driving device 3 is driven by drive shaft 4
Gear 8 and connecting rod 10 rotate, and the connecting rod 10 is through culture 13 side wall of foundation trench and 10 end of connecting rod is provided with impeller 14,
The cell culture well 2 and the setting of preformed hole 15 are inside culture foundation trench 13.
The culture foundation trench 13 of the dynamic cell culture plate, which is square, to be symmetricly set on supporting plate 1, and drive shaft 4 is distributed in
Two sides, the driving device 3 are set on support base 6, and the support base 6 is set on supporting plate 1, and described 4 one end of drive shaft connects
Driving device 3 is connect, the other end of drive shaft 4 is flexibly connected with the support rod 5 being arranged on supporting plate 1, and 4 surface of drive shaft is equidistant
External screw thread 9 is set, and the gear 8 being fixedly installed in the middle part of the external screw thread 9 and connecting rod 10 engages, the both ends point of the connecting rod 10
Not Guan Chuan corresponding 13 side wall of culture foundation trench and end be provided with impeller 14.Culture foundation trench 13 is square distribution, is adapted to
More experimental occasions, while the quantity for cultivating foundation trench 13 experimental can need to increase or decrease in even numbers.
One cell culture well 2 of setting and a preformed hole 15 in the culture foundation trench 13 of the distribution that is square.Cell training
It supports hole 2 and is used to cultivate attached cell, setting preformed hole 15 both can use its contour structures and form culture in culture foundation trench 13
The special flow passage of base, can also in preformed hole 15 static culture cell, or the controlled observation area as cell culture.
The connecting rod 10 of the dynamic cell culture plate is equipped with rubber seal 18 through the position of culture 13 side wall of foundation trench.
In this way in 10 rotation process of connecting rod, the abrasion of culture 13 side wall of foundation trench and 10 junction of connecting rod can be both reduced, is eliminated
Noise, and sealing function can be played, prevent culture medium from leaking into outside, while also can be used as a fulcrum of connecting rod 10,
Ensure the dynamic balancing of connecting rod 10.
The driving device 3 of the dynamic cell culture plate is electric machine, and battery is used to provide power supply, motor dress for motor
Bottom set portion is fixedly connected with support base 6, and the side of electric machine connects drive shaft 4, inside the support base 6 setting battery case and
Power supply line, power supply line connect motor and battery case, power switch 20 are arranged outside support base 6.It is driven using electric machine, structure
Simply, small in size, it is easy to use, run lasting stability.
As shown in figure 12,2 sidewall symmetry of cell culture well is provided with through-hole 17, matches inside each cell culture well 2
A dismountable circular sleeve 16 is covered, the height of sleeve 16 is slightly above cell culture well 2, convenient for taking out of cell culture well 2
Out.The cell culture well 2 is suitble to culture attached cell, tests initial stage, sleeve 16 is first put into cell culture well 2, thin in this way
The through-hole 17 of 2 side wall of born of the same parents' culture hole is blocked, and the culture medium in cell culture well 2 is in closing stationary state, and cell can be thin
Carried out in 2 internal backplane of born of the same parents' culture hole it is adherent, after cell completely it is adherent after, take away sleeve 16, the training in such cell culture well 2
Culture medium in feeding base and culture foundation trench 13, which can converge, to be integrated.
As shown in Figure 13 and Figure 14, the impeller 14 of the dynamic cell culture plate is double shrouded wheel, and impeller 14 is by front/rear cover
Plate and arc shaped blade composition, arc shaped blade are fixed between forward and backward cover board.Double shrouded wheel efficiency is higher, while can be to prevent
Only culture solution splashes around, it is ensured that culture medium smooth flow under the effect of impeller 14.
Dynamic cell culture plate disclosed by the invention when in use, starts driving device 3, and driving device 3 passes through drive shaft 4
Band moving gear 8 rotates, and gear 8 drives the impeller 14 of 10 end of connecting rod 10 and connecting rod to rotate again, under the effect of impeller 14, training
Feeding base can flow in culture foundation trench 13, and the cell in such cell hole is at dynamic environment, realize that cell dynamic is trained
It supports.
Embodiment 2:
As shown in figures 1 and 3, embodiment 2 and the difference of embodiment 1 are: the driving dress of the dynamic cell culture plate
Set 3 differences.
As shown in Figure 3 and Figure 4, the driving device 3 of the dynamic cell culture plate of present embodiment is clockwork, clockwork spring dress
Bottom set portion is fixedly connected with support base 6, and the side of clockwork connects drive shaft 4, and clockwork spring hand is arranged in the other side of clockwork
Handle 19.It being driven using clockwork, is not necessarily to energy consumption, structure is simple, and it is small in size, it is easy to use.
Embodiment 3:
As shown in Fig. 5, Fig. 6 and Figure 15, a kind of dynamic cell culture plate, including supporting plate 1, cell culture well 2, driving device 3, branch
Support seat 6, drive shaft 4, gear 8, connecting rod 10, culture foundation trench 13, preformed hole 15 and impeller 14, the driving device 3 and branch
Support seat 6 is fixedly connected, and the drive shaft 4 is connect by gear 8 with connecting rod 10, and the driving device 3 is driven by drive shaft 4
Gear 8 and connecting rod 10 rotate, and the connecting rod 10 is through culture 13 side wall of foundation trench and 10 end of connecting rod is provided with impeller 14,
The cell culture well 2 and the setting of preformed hole 15 are inside culture foundation trench 13.
The culture foundation trench 13 of the dynamic cell culture plate, which is square, to be symmetricly set on supporting plate 1, and drive shaft 4 is distributed in
Two sides, the driving device 3 are set on support base 6, and the support base 6 is set on supporting plate 1, and described 4 one end of drive shaft connects
Driving device 3 is connect, the other end of drive shaft 4 is flexibly connected with the support rod 5 being arranged on supporting plate 1, and 4 surface of drive shaft is equidistant
External screw thread 9 is set, and the gear 8 being fixedly installed in the middle part of the external screw thread 9 and connecting rod 10 engages, the both ends point of the connecting rod 10
Not Guan Chuan corresponding 13 side wall of culture foundation trench and end be provided with impeller 14.Culture foundation trench 13 is square distribution, is adapted to
More experimental occasions, while the quantity for cultivating foundation trench 13 experimental can need to increase or decrease in even numbers.
Two cell culture wells 2 of setting and a preformed hole 15 in the culture foundation trench 13 of the distribution that is square.Setting two
The purpose of a cell culture well 2 is for cultivating two kinds of cells, and each cell culture well 2 cultivates a kind of cell, such two kinds thin
Born of the same parents live in the culture medium of same flowing, can analyze interaction and influence between two kinds of cells of observation.Setting is reserved
Hole 15 both can use the special flow passage that its contour structures forms culture medium in culture foundation trench 13, can also be in preformed hole 15
Static culture cell, or the controlled observation area as cell culture.
The connecting rod 10 of the dynamic cell culture plate is equipped with rubber seal 18 through the position of culture 13 side wall of foundation trench.
In this way in 10 rotation process of connecting rod, the abrasion of culture 13 side wall of foundation trench and 10 junction of connecting rod can be both reduced, is eliminated
Noise, and sealing function can be played, prevent culture medium from leaking into outside, while also can be used as a fulcrum of connecting rod 10,
Ensure the dynamic balancing of connecting rod 10.
It is described culture foundation trench 13 be square distribution dynamic cell culture plate driving device 3 be electric machine, using electricity
Pond provides power supply for motor, and electric machine bottom is fixedly connected with support base 6, and the side of electric machine connects drive shaft 4, described
Battery case and power supply line are set inside support base 6, and power supply line connects motor and battery case, power switch is arranged outside support base 6
20.It being driven using electric machine, structure is simple, and it is small in size, it is easy to use, run lasting stability.
As shown in figure 12,2 sidewall symmetry of cell culture well of the dynamic cell culture plate is provided with through-hole 17, Mei Gexi
The mating dismountable circular sleeve 16 in the inside of born of the same parents' culture hole 2, the height of sleeve 16 are slightly above cell culture well 2, be convenient for from
It is taken out in cell culture well 2.The cell culture well 2 is suitble to culture attached cell, tests initial stage, sleeve 16 is first put into cell
The through-hole 17 of culture hole 2, such 2 side wall of cell culture well is blocked, and the culture medium in cell culture well 2, which is in, closes static shape
State, cell can be carried out in 2 internal backplane of cell culture well it is adherent, after cell completely it is adherent after, take away sleeve 16, this like cell
The culture medium in culture medium and culture foundation trench 13 in culture hole 2, which can converge, to be integrated.
As shown in Figure 13 and Figure 14, the impeller 14 of the dynamic cell culture plate is double shrouded wheel, and impeller 14 is by front/rear cover
Plate and arc shaped blade composition, arc shaped blade are fixed between forward and backward cover board.Double shrouded wheel efficiency is higher, while can be to prevent
Only culture solution splashes around, it is ensured that culture medium smooth flow under the effect of impeller 14.
Dynamic cell culture plate disclosed by the invention when in use, starts driving device 3, and driving device 3 passes through drive shaft 4
Band moving gear 8 rotates, and gear 8 drives the impeller 14 of 10 end of connecting rod 10 and connecting rod to rotate again, under the effect of impeller 14, training
Feeding base can flow in culture foundation trench 13, and the cell in such cell hole is at dynamic environment, realize that cell dynamic is trained
It supports.
Embodiment 4:
As shown in figure 5 and figure 7, embodiment 4 and the difference of embodiment 3 are: the driving dress of the dynamic cell culture plate
Set 3 differences.
As shown in Figure 7 and Figure 8, the driving device 3 of the dynamic cell culture plate be clockwork, clockwork bottom with
Support base 6 is fixedly connected, and the side of clockwork connects drive shaft 4, and clockwork spring handle 19 is arranged in the other side of clockwork.Using
Clockwork driving, is not necessarily to energy consumption, and structure is simple, small in size, easy to use.
Embodiment 5:
As shown in Fig. 9, Figure 11 and Figure 16, a kind of dynamic cell culture plate, including supporting plate 1, cell culture well 2, driving device, branch
Support seat 6, drive shaft 4, gear 8, connecting rod 10, culture foundation trench 13, preformed hole 15 and impeller 14, the dynamic cell culture plate
Culture foundation trench 13 it is rounded be arranged on supporting plate 1, the driving device is installed on inside support base 6, and the support base 6 passes through
Support column 7 is set to the top of supporting plate 1, and described 4 one end of drive shaft connects driving device through 6 bottom of support base, drive shaft 4 it is another
One end be arranged first bevel gear 11, the connecting rod 10 one end setting second bevel gear 12 and with 4 end of drive shaft setting
First bevel gear 11 engages, and the driving device drives first bevel gear 11 and connecting rod 10 to rotate by drive shaft 4, connecting rod
10 other end is through corresponding 13 side wall of culture foundation trench and end is provided with impeller 14.
There are a cell culture well 2 and a preformed hole 15 in the culture foundation trench 13.The rounded distribution of foundation trench 13 is cultivated,
More experimental occasions are adapted to, the quantity for cultivating foundation trench 13 experimental can need to increase or decrease, and only need simultaneously
Increase or decrease the quantity of the connecting rod 10 with second bevel gear 12.
The connecting rod 10 of the dynamic cell culture plate is equipped with rubber seal 18 through the position of culture 13 side wall of foundation trench.
In this way in 10 rotation process of connecting rod, the abrasion of culture 13 side wall of foundation trench and 10 junction of connecting rod can be both reduced, is eliminated
Noise, and sealing function can be played, prevent culture medium from leaking into outside, while also can be used as a fulcrum of connecting rod 10,
Ensure the dynamic balancing of connecting rod 10.
The driving device of the dynamic cell culture plate is electric machine, and battery is used to provide power supply, motor dress for motor
It sets and is installed on inside support base 6, the drive shaft 4 is connect through 6 bottom of support base with electric machine, outside the support base 6
Power switch 20 is set, battery case and power supply line are additionally provided with inside support base 6, power supply line connects motor and battery case.Using
Electric machine driving, structure is simple, small in size, easy to use, runs lasting stability.
As shown in figure 12,2 sidewall symmetry of cell culture well of the dynamic cell culture plate is provided with through-hole 17, Mei Gexi
The mating dismountable circular sleeve 16 in the inside of born of the same parents' culture hole 2, the height of sleeve 16 are slightly above cell culture well 2, be convenient for from
It is taken out in cell culture well 2.The cell culture well 2 is suitble to culture attached cell, tests initial stage, sleeve 16 is first put into cell
The through-hole 17 of culture hole 2, such 2 side wall of cell culture well is blocked, and the culture medium in cell culture well 2, which is in, closes static shape
State, cell can be carried out in 2 internal backplane of cell culture well it is adherent, after cell completely it is adherent after, take away sleeve 16, this like cell
The culture medium in culture medium and culture foundation trench 13 in culture hole 2, which can converge, to be integrated.
As shown in Figure 13 and Figure 14, the impeller 14 of the dynamic cell culture plate is double shrouded wheel, and impeller 14 is by front/rear cover
Plate and arc shaped blade composition, arc shaped blade are fixed between forward and backward cover board.Double shrouded wheel efficiency is higher, while can be to prevent
Only culture solution splashes around, it is ensured that culture medium smooth flow under the effect of impeller 14.
Dynamic cell culture plate disclosed by the invention when in use, starts driving device, and driving device passes through 4 band of drive shaft
Dynamic first bevel gear 11 rotates, and first bevel gear 11 drives second bevel gear 12 to rotate, and second bevel gear 12 drives connecting rod again
10 and 10 end of connecting rod impeller 14 rotate, impeller 14 effect under, culture medium can culture foundation trench 13 in flow, this
Cell in like cell hole is at dynamic environment, realizes cell dynamic cultivation.
Embodiment 6:
As shown in Figure 9 and Figure 10, embodiment 6 and the difference of embodiment 5 are: the driving dress of the dynamic cell culture plate
Set difference.
The driving device of the dynamic cell culture plate is clockwork, and clockwork is installed on inside support base 6, is driven
Axis 4 is connected through 6 bottom of support base and the side of clockwork, and clockwork spring handle 19 is through 6 top of support base and clockwork
Other side connection.It being driven using clockwork, is not necessarily to energy consumption, structure is simple, and it is small in size, it is easy to use.
In figure, description positional relationship only for illustration, should not be understood as the limitation to this patent；Obviously, this hair
Bright the above embodiment is merely an example for clearly illustrating the present invention, and is not to embodiments of the present invention
It limits.For those of ordinary skill in the art, other different forms can also be made on the basis of the above description
Variation or variation.There is no necessity and possibility to exhaust all the enbodiments.It is all the spirit and principles in the present invention it
Any modifications, equivalent replacements, and improvements made by interior etc., should all be included in the scope of protection of the claims of the present invention.
Claims (10)
1. a kind of dynamic cell culture plate, including supporting plate, cell culture well, which is characterized in that the dynamic cell culture plate is also
Including driving device, support base, drive shaft, gear, connecting rod, culture foundation trench, preformed hole and impeller, the driving device with
Support base is fixedly connected, and the drive shaft is connect by gear with connecting rod, and the driving device passes through driving shaft driven gear
It is rotated with connecting rod, the connecting rod is through culture medium groove sidewall and connection boom end is provided with impeller, the cell culture well
With preformed hole setting inside culture foundation trench.
2. dynamic cell culture plate according to claim 1, which is characterized in that the cell culture well sidewall symmetry setting
There are through-hole, a mating dismountable circular sleeve inside each cell culture well.
3. dynamic cell culture plate according to claim 2, which is characterized in that the culture foundation trench is square arranged symmetrically
On supporting plate, the driving device is set on support base, and the support base is set on supporting plate, drive shaft one end connection
Driving device, the other end of drive shaft are flexibly connected with the support rod being arranged on supporting plate, and drive shaft surface is equidistantly positioned outer
Screw thread, the external screw thread are engaged with the gear being fixedly installed in the middle part of connecting rod, and the both ends of the connecting rod extend through corresponding
Culture medium groove sidewall and end be provided with impeller.
4. dynamic cell culture plate according to claim 3, which is characterized in that be provided in the culture foundation trench one thin
Born of the same parents' culture hole and a preformed hole.
5. dynamic cell culture plate according to claim 3, which is characterized in that there are two thin for setting in the culture foundation trench
Born of the same parents' culture hole and a preformed hole.
6. dynamic cell culture plate according to claim 2, which is characterized in that the rounded setting of culture foundation trench is being held in the palm
On plate, the driving device is set to inside support base, and the support base is set to above supporting plate by support column, the driving
Axis one end connects driving device through support base bottom, and the other end of drive shaft is arranged first bevel gear, and the one of the connecting rod
End setting second bevel gear is simultaneously engaged with the first bevel gear of driveshaft end setting, and the other end of connecting rod runs through corresponding training
It supports foundation trench side wall and end is provided with impeller.
7. dynamic cell culture plate according to claim 1-6, which is characterized in that the connecting rod is through culture
The position of foundation trench side wall is provided with rubber seal.
8. dynamic cell culture plate according to claim 7, which is characterized in that the impeller is double shrouded wheel.
9. dynamic cell culture plate according to claim 8, which is characterized in that the driving device is electric machine, institute
State the side connection drive shaft of electric machine.
10. dynamic cell culture plate according to claim 8, which is characterized in that the driving device is clockwork, institute
The side connection drive shaft of clockwork is stated, the other side of clockwork is provided with clockwork spring handle.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201811243800.0A CN109666587A (en) | 2018-10-24 | 2018-10-24 | A kind of dynamic cell culture plate |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201811243800.0A CN109666587A (en) | 2018-10-24 | 2018-10-24 | A kind of dynamic cell culture plate |
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| CN201811243800.0A Pending CN109666587A (en) | 2018-10-24 | 2018-10-24 | A kind of dynamic cell culture plate |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109929761A (en) * | 2019-04-30 | 2019-06-25 | 杭州捷诺飞生物科技股份有限公司 | Cell dynamic cultivation chip and cell dynamic cultivation device |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101270332A (en) * | 2008-04-30 | 2008-09-24 | 钟诗龙 | Apparatus and method for cultivation and process of cell or tissue |
| CN102329726A (en) * | 2011-09-01 | 2012-01-25 | 山西医科大学 | Cell culture dish |
| US20150093819A1 (en) * | 2013-09-29 | 2015-04-02 | Yongxin Zhang | Bioreactor System and Methods for Alternative Cell Culture between Static and Dynamic |
| WO2017083705A1 (en) * | 2015-11-13 | 2017-05-18 | The Johns Hopkins University | Cell culture system and method of use thereof |
| CN207391440U (en) * | 2017-09-06 | 2018-05-22 | 中国人民解放军第二军医大学 | Cell co-culture device |
| CN209162089U (en) * | 2018-10-24 | 2019-07-26 | 海南医学院 | A kind of dynamic cell culture plate |
-
2018
- 2018-10-24 CN CN201811243800.0A patent/CN109666587A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101270332A (en) * | 2008-04-30 | 2008-09-24 | 钟诗龙 | Apparatus and method for cultivation and process of cell or tissue |
| CN102329726A (en) * | 2011-09-01 | 2012-01-25 | 山西医科大学 | Cell culture dish |
| US20150093819A1 (en) * | 2013-09-29 | 2015-04-02 | Yongxin Zhang | Bioreactor System and Methods for Alternative Cell Culture between Static and Dynamic |
| WO2017083705A1 (en) * | 2015-11-13 | 2017-05-18 | The Johns Hopkins University | Cell culture system and method of use thereof |
| CN207391440U (en) * | 2017-09-06 | 2018-05-22 | 中国人民解放军第二军医大学 | Cell co-culture device |
| CN209162089U (en) * | 2018-10-24 | 2019-07-26 | 海南医学院 | A kind of dynamic cell culture plate |
Non-Patent Citations (1)
| Title |
|---|
| 何辉, 樊瑜波: "动物细胞培养用生物反应器及其力学环境", 生物医学工程学杂志, vol. 21, no. 03, pages 498 - 501 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109929761A (en) * | 2019-04-30 | 2019-06-25 | 杭州捷诺飞生物科技股份有限公司 | Cell dynamic cultivation chip and cell dynamic cultivation device |
| CN109929761B (en) * | 2019-04-30 | 2024-02-02 | 杭州捷诺飞生物科技股份有限公司 | Cell dynamic culture chip and cell dynamic culture device |
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