CN111500410A

CN111500410A - PCR mobile device and PCR appearance

Info

Publication number: CN111500410A
Application number: CN202010341161.2A
Authority: CN
Inventors: 曹进涛; 李冬; 贺贤汉
Original assignee: Hangzhou Bioer Technology Co ltd
Current assignee: Hangzhou Bioer Technology Co ltd
Priority date: 2020-04-26
Filing date: 2020-04-26
Publication date: 2020-08-07
Anticipated expiration: 2040-04-26
Also published as: CN111500410B

Abstract

The invention provides a PCR mobile device and a PCR instrument, relating to the technical field of PCR detection, wherein the device comprises: the PCR device comprises a first mounting part, a first telescopic part and a second telescopic part, wherein the first side of the first telescopic part is movably connected with the PCR body, and the second side of the first telescopic part bears the first mounting part; the first telescopic part is used for moving the first mounting part from the outside of the PCR body into the PCR body or moving the first mounting part out of the PCR body; the first mounting part comprises a movable seat for placing the sample module; the first end of the second telescopic part is connected with the movable seat, the second end of the second telescopic part is connected with the first telescopic part, and the second telescopic part is used for moving the movable seat upwards or downwards. The device has alleviated the poor stability that leads to the PCR device because of optical system's removal, has detected the low problem of precision, has realized improving the beneficial effect that detects precision and detection efficiency.

Description

PCR mobile device and PCR appearance

Technical Field

The invention relates to the technical field of PCR detection, in particular to a PCR mobile device and a PCR instrument.

Background

The basic principle of the PCR (polymerase Chain reaction) instrument is to amplify DNA fragments by heating and cooling cycles, and the core of the instrument is a temperature control module. A Real-time fluorescent quantitative nucleic acid amplification detection System (Real-time quantitative PCR detection System), namely a QPCR instrument, is additionally provided with an optical excitation and detection System on the basis of the traditional PCR instrument. Most of QPCR appearance temperature control module is fixed in the existing market, reaches the purpose with the test tube pressfitting through the downward motion of optical module and hot lid. However, such a scheme often has the problems of poor stability and low detection accuracy of the PCR device due to the movement of the optical system.

Disclosure of Invention

In view of the above, the present invention is directed to a PCR moving apparatus and a PCR instrument, so as to solve the problems of poor stability and low detection accuracy of the conventional PCR apparatus.

In order to achieve the above purpose, the embodiment of the present invention adopts the following technical solutions:

in a first aspect, an embodiment of the present invention provides a PCR mobile apparatus, including: the first mounting component, the first telescopic component and the second telescopic component; the first side of the first telescopic part is movably connected with the PCR body, the second side of the first telescopic part is used for bearing the first mounting part, and the first telescopic part is used for moving the first mounting part from the outside of the PCR body into the PCR body or moving the first mounting part out of the PCR body; the first mounting part comprises a movable seat for placing the sample module; the first end of the second telescopic component is connected with the movable seat, the second end of the second telescopic component is connected with the first telescopic component, and the second telescopic component is used for moving the movable seat upwards or downwards.

In some embodiments, the apparatus further comprises: the motor is connected with the driving part, and the driving part is connected with the second telescopic part; the motor is used for driving the driving component, so that the second telescopic component, the first telescopic component and the first mounting component move.

In some embodiments, the second telescoping member further comprises a third end, the third end of the second telescoping member being movably connected to the second side of the first telescoping member.

In some embodiments, the first mounting member further comprises a housing having a channel disposed thereon; the movable seat is arranged in the shell, and the second telescopic part moves the movable seat along the guide groove.

In some embodiments, the second telescoping member comprises: the first connecting rod is connected with the first end of the second connecting rod, the second end of the second connecting rod is hinged with one end of the third connecting rod, and meanwhile, the second end of the second connecting rod is hinged with one end of the fourth connecting rod.

In some embodiments, the second link is provided with an avoiding groove, and when the first end of the second telescopic member is located at the bottom end of the guide groove, the avoiding groove is located corresponding to the guide groove.

In some embodiments, the PCR body comprises a second mounting feature; the first telescopic member includes: a first guide rail and a second guide rail; the second guide rail is fixed on the first guide rail, and the first guide rail is connected with the second mounting component through the sliding block.

In some embodiments, the driving part drives the second guide rail, the first guide rail and the first mounting part to perform a first horizontal movement relative to the second mounting part through the first connecting rod; the driving part drives the first connecting rod and the second connecting rod to perform second horizontal movement relative to the first guide rail; the driving part drives the end part of the fourth connecting rod to perform first vertical movement in the guide groove through second horizontal movement of the first connecting rod and the second connecting rod; and the first horizontal movement and the second horizontal movement do not occur simultaneously, and the second horizontal movement is carried out after the first horizontal movement is stopped, or the first horizontal movement is carried out after the second horizontal movement is stopped.

In some embodiments, the first telescoping member further comprises a first stop disposed at one end of the first guide rail; the second mounting part further comprises a second stopper, and the position of the first stopper corresponds to the position of the second stopper.

In some embodiments, the first horizontal movement is driven by a motor, the speed of the first horizontal movement comprising a first speed and a second speed; the first speed is greater than the second speed; the first vertical motion is driven by the first horizontal motion; the first speed is greater than the speed of the first vertical movement.

According to the PCR mobile device provided by the embodiment, the impact force of the device at the motion cut-off time is reduced by reducing the motion speed, the stability of the device is improved, and the service life of the device is prolonged.

In some embodiments, the first mounting component further comprises a third rail; the third guide rail is arranged on the inner side of the first mounting part and is parallel to the guide groove, and the movable seat is arranged on the third guide rail through a sliding block; the first end of the second telescopic part drives the movable seat to slide on the third guide rail.

In some embodiments, the first mounting member further comprises a buffer member disposed between the moving seat and the sample module; the buffer component comprises a guide rod and a spring; the guide rod is arranged above the movable seat, and the spring is sleeved outside the guide rod; the top end of the movable seat is connected with one end of the guide rod, and the other end of the guide rod is connected with the sample module.

In some embodiments, the spring has a first amount of deformation and a second amount of deformation in the vertical direction; the second type of variation is used to provide pressure between the heat cap and the sample tube.

The embodiment provides a PCR mobile device, realizes through the motion of control sample module that the hot lid forms sufficient pressure to it, therefore can make temperature control module in the sample module and sample test tube between have good heat transfer efficiency, sufficient pressure has still avoided sample reagent to evaporate out from the test tube simultaneously, has improved detection accuracy.

In a second aspect, an embodiment of the present invention provides a PCR instrument, including the PCR mobile device and the sample module described in any one of the first aspects.

The embodiment of the invention provides a PCR mobile device and a PCR instrument, wherein the device comprises: the PCR device comprises a first mounting part, a first telescopic part and a second telescopic part, wherein the first side of the first telescopic part is movably connected with the PCR body, and the second side of the first telescopic part bears the first mounting part; the first telescopic part is used for moving the first mounting part from the outside of the PCR body into the PCR body or moving the first mounting part out of the PCR body; the first mounting part comprises a movable seat for placing the sample module; the first end of the second telescopic part is connected with the movable seat, the second end of the second telescopic part is connected with the first telescopic part, and the second telescopic part is used for moving the movable seat upwards or downwards. The device has alleviated the poor stability that leads to the PCR device because of optical system's removal, has detected the low problem of precision, has further realized improving the beneficial effect that detects precision and detection efficiency.

In addition, the PCR moving device provided by the embodiment of the invention realizes the movement of the sample module for bearing the sample in the horizontal and vertical directions, does not need to stretch into the PCR instrument to take and place the sample, and is time-saving, labor-saving and very convenient; by slowing down the movement speed when the device is about to stop, the impact force of the device when the device stops moving is reduced, the stability of the device is improved, and the service life of the device is prolonged; the movement of the sample module is controlled to realize that the heat cover forms enough pressure on the sample tube, so that the temperature control module in the sample module and the sample tube have good heat transfer efficiency, and meanwhile, the enough pressure also prevents a sample reagent from evaporating from the test tube, and the detection accuracy is improved.

Drawings

In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, and it is obvious that the drawings in the following description are some embodiments of the present invention, and other drawings can be obtained by those skilled in the art without creative efforts.

FIG. 1 is a schematic side view of a PCR mobile device according to an embodiment of the present invention;

FIG. 2 is a top view of a PCR mobile device according to an embodiment of the present invention;

FIG. 3 is a top view of another PCR mobile device according to an embodiment of the present invention;

FIG. 4 is a schematic side view of another PCR mobile device according to an embodiment of the present invention;

FIG. 5 is a schematic diagram of an internal structure of a first mounting part of a PCR mobile device according to an embodiment of the present invention;

fig. 6 is a schematic diagram illustrating a moving state of a PCR mobile apparatus according to an embodiment of the invention.

Icon: 100-PCR body; 110-a first telescopic member; 210-a second telescopic member; 310-a first mounting part; 112-a first guide rail; 113-a second guide rail; 114-a second stop; 116-a first stop; 211-a first link; 212-a second link; 213-third link; 214-a fourth link; 311-a housing; 312-a mobile seat; 313-a guide slot; 314-a third guide rail; 315-a slider; 316-a guide bar; 317-a spring; 410-a drive member; 411-motor.

Detailed Description

To make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions of the present invention will be clearly and completely described below with reference to the accompanying drawings, and it is apparent that the described embodiments are some, but not all embodiments of the present invention. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention. It should be noted that the embodiments and features of the embodiments in the present application may be combined with each other without conflict. The present application will be described in detail below with reference to the embodiments with reference to the attached drawings.

The PCR instrument has the basic principle that DNA fragments are amplified through heating and cooling circulation, the core of the PCR instrument is a temperature control module, and an optical excitation and detection system is added on the basis of the traditional PCR instrument by the QPCR instrument. Most of QPCR appearance temperature control module is fixed in the existing market, reaches the purpose with the test tube pressfitting through the downward motion of optical module and hot lid. However, such a scheme often has the problems of poor stability and low detection accuracy of the PCR device due to the movement of the optical system.

To facilitate understanding of the present embodiment, a PCR moving apparatus disclosed in the present embodiment is first described, referring to fig. 1 and fig. 2, which are schematic structural diagrams of the PCR moving apparatus, wherein fig. 1 (a) and (B) are both side views of the apparatus, and fig. 2 is a top view of the apparatus.

The device includes: a first mounting member 310, a first telescopic member 110 and a second telescopic member 210; the first side of the first telescopic part 110 is movably connected with the PCR body, the second side of the first telescopic part 110 carries the first mounting part 310, and the first telescopic part 110 is used for moving the first mounting part 310 from the outside of the PCR body into the PCR body, or moving the first mounting part 310 from the inside of the PCR body to the outside of the PCR body;

the first mounting member 310 includes a moving seat 312, the moving seat 312 being used to place the sample module;

the first end of the second telescopic member 210 is connected to the moving base 312, the second end of the second telescopic member 210 is connected to the first telescopic member, and the second telescopic member 210 is used for moving the moving base 312 upwards or downwards.

According to the PCR moving device provided by the embodiment, the horizontal movement of the device is realized through the first telescopic part, and the sample module placed in the device is moved into the PCR body or moved out of the PCR body; vertical motion through the flexible part of second realization device reciprocates the sample module in PCR body is inside to avoided optical system's removal to lead to stability and detection precision to reduce, improved the detection precision and the detection efficiency of system.

Wherein the first mounting part can be used for carrying the sample module and realizing the movement of the sample module through the moving device. It should be noted that, the PCR body further includes a detection structure, such as some optical detection structures, etc., the detection structure is generally disposed above the PCR moving device, and when the moving device moves the sample upwards to a corresponding position, the detection structure detects the sample, and in the process, the optical detection structure is always kept still, thereby avoiding the movement of the optical system, and improving the detection precision and detection efficiency of the PCR instrument. The sample module comprises a sample, optionally a temperature control module, and other structures such as a test tube holder for carrying the sample.

Referring to part (a) of fig. 1, a first side of the first telescopic member 110 may be represented as a lower side of the first telescopic member; the second side of the first telescopic member may be the side carrying the first mounting member 310, i.e. the upper side.

In some embodiments, the first telescoping member comprises: the second guide rail is fixed on the first guide rail; the PCR body comprises a second mounting part, the first rail is connected with the second mounting part through a slide block, for example, the second mounting part can be a rail mounting plate.

Wherein the length of the second guide rail is generally smaller than that of the first guide rail, the second guide rail can be fixedly installed at one end of the upper side of the first guide rail, as shown in fig. 3, and the second guide rail 113 is installed at one end of the first guide rail 112 close to the motor 411. The first rail is connected to the second mounting member on the lower side thereof through a slider, and the slider may be provided on the lower side of the first rail or on the second mounting member for realizing the relative movement of the first rail with respect to the second mounting member, and the mounting position of the slider is not limited herein.

The first telescopic parts may be two groups, and are respectively disposed at two sides of the first mounting part, as shown in fig. 2 and 3, the first telescopic parts may include two first guide rails, which are respectively connected with the second mounting part; the first telescopic part can also comprise two second guide rails which are respectively fixed on the two first guide rails. The guide rails may be elongated, and the length of the first guide rail is generally longer than that of the second guide rail.

When first flexible parts are two sets of, the second flexible parts are corresponding also sets up to two sets ofly, sets up at the bilateral symmetry of sample module respectively, can utilize a plurality of connecting rods and the guide rail of both sides to realize the motion of sample module in level and vertical direction.

In some embodiments, the first mounting member further includes a housing 311 shown in fig. 4, the housing 311 having a guide groove 313 provided thereon; the movable base is disposed in the housing 311, and the second telescopic member moves the movable base along the guide groove 313.

The first end of the second telescopic component and the moving seat can be guided by arranging the guide groove on the shell of the first mounting component, so that the first end of the second telescopic component and the moving seat can move along the direction of the guide groove without deviation. Further, the guide slot on the first installation component shell can be certain contained angle setting with first flexible part, and a preferred mode is the first flexible part setting of guide slot perpendicular to, and the first end of the flexible part of second of being convenient for moves in vertical direction along the guide slot to drive the removal seat of being connected with it, further drive the device that removes the seat top and move in vertical direction, and the device that removes the seat top can be sample module etc..

As an alternative embodiment, referring to a schematic side view of another PCR mobile device shown in fig. 4, the second telescopic member includes: the first end of the second link 212 is connected with the first end of the first link 211, the second end of the second link 212 is hinged with one end of the third link 213, and the second end of the second link 212 is hinged with one end of the fourth link 214.

Specifically, the first end of the second telescopic member may be an end of the fourth link 214, which is not hinged to the second link, and the first end is connected to the movable base; the second end of the second telescopic member, which may be the end of the third link 213 not articulated to the second link, is connected to the first rail in the first telescopic member. The third end of the second telescopic member may be an end of a first link 211, one end of the first link 211 is movably connected to the second side of the first telescopic member, and the other end of the first link 211 is connected to the non-hinged end of the second link 212.

As an optional implementation manner, an avoiding groove is formed in the second connecting rod, and when the first end of the second telescopic member is located at the bottom end of the guide groove, the position of the avoiding groove corresponds to the position of the guide groove.

Specifically, when the end of the fourth connecting rod, which is not hinged to the second connecting rod and the third connecting rod, moves to the lowest end of the guide groove, the avoidance groove in the second connecting rod corresponds to the guide groove, and the avoidance groove is arranged to prevent the second connecting rod from generating obstacles, so that the end of the fourth connecting rod, which is connected to the movable seat, can smoothly reach the lowest end of the guide groove.

In some embodiments, referring to a top view of a PCR displacement apparatus shown in fig. 2, the apparatus further comprises: the driving part 410 and the motor 411, the motor 411 is connected with the driving part 410, and the driving part 410 is connected with the second telescopic part; the motor 411 is used for controlling the driving part 410 to realize the movement of the second telescopic part, the first telescopic part and the first mounting part.

The driving part can be a driving rod and is connected with one end of the second telescopic part, and particularly can be connected with one end of the first connecting rod; the motor can be a linear motor capable of adjusting the rotating speed, and the driving rod is pushed by the screw rod to move in the horizontal direction. The driving part moves in the horizontal direction under the driving action of the motor, so that the second telescopic part and the second telescopic part are driven to drive the first telescopic part and the first mounting part which are connected with the second telescopic part to move in the horizontal direction.

As an optional implementation manner, the driving part drives the second guide rail, the first guide rail and the first mounting part through the first connecting rod to perform a first horizontal movement relative to the second mounting part; the driving part drives the first connecting rod and the second connecting rod to perform second horizontal movement relative to the first guide rail; the driving part drives the end part of the fourth connecting rod to perform first vertical movement in the guide groove through second horizontal movement of the first connecting rod and the second connecting rod; and the first horizontal movement and the second horizontal movement do not occur simultaneously, and the second horizontal movement is carried out after the first horizontal movement is stopped, or the first horizontal movement is carried out after the second horizontal movement is stopped.

When the sample module is outside the PCR body, the device moves the sample module into the PCR body through the first horizontal movement of the first telescopic part, then generates the first vertical movement through the second horizontal movement of the second telescopic part, and moves the sample module upwards in the PCR body, so that the sample module is in contact with other devices of the PCR body, such as a heat cover, a detection module and the like, and the detection preparation work of the sample is completed.

When the sample test is completed, the second telescopic part is controlled by the motor and the driving part to realize second horizontal motion and first vertical motion, the sample module is moved downwards in the PCR body to be separated from other devices of the PCR body, the second horizontal motion and the first vertical motion stop when the sample module is moved to a specified position, and the first telescopic part is controlled by the motor and the driving part to realize the first horizontal motion to move the sample module to the outside of the PCR body. The second horizontal movement may occur simultaneously with the first vertical movement.

The PCR mobile device that this embodiment provided has realized bearing the sample module at the ascending motion of level and vertical two directions, thereby can remove the sample module and carry out getting of sample to the PCR appearance outside and put, need not stretch out the hand and probe into the inside getting of carrying out the sample of PCR appearance and put, labour saving and time saving and very convenient.

A specific connection mode of the device is shown in FIG. 4, wherein a first guide rail is movably connected with a second mounting part (e.g. a guide rail mounting plate) of the PCR body through a sliding block, the second guide rail is connected with a first connecting rod 211 through a sliding block, the front end of the first connecting rod 211 is connected with a driving part 410, and the rear end of the first connecting rod 211 is movably connected with the front end of a second connecting rod 212; the rear end of the second link 212 is hinged to both the first end of the third link 213 and the first end of the fourth link 214; the second end of the third link 213 is fixedly connected to the first guide rail, and the second end of the fourth link 214 is movably disposed in the guide groove and connected to the movable base.

The motor 411 may drive the driving member 410 connected thereto, the first link 211 fixed to the driving member, the second guide rail connected to the first link, the first guide rail fixed to the second guide rail, the first mounting member fixed to the first guide rail, and the sample module mounted on the first mounting member, and perform a first horizontal movement with respect to the second mounting member under the driving of the driving member 410.

As an optional implementation manner, the first telescopic component further comprises a first stop block, and the first stop block is arranged at one end of the first guide rail; the second mounting part further comprises a second stopper, and the position of the first stopper corresponds to the position of the second stopper.

Referring to fig. 3, a first stopper 116 may be disposed at a rear end of the first rail, i.e., an end away from the second rail, and a second stopper 114 may be disposed on the second mounting part, corresponding to a position of the first stopper. The first guide rail performs a first horizontal movement from left to right in the direction shown in the figure, and when the first mounting part and the devices such as the sample module carried by the first mounting part all enter the PCR body, the second stopper contacts with the first stopper to prevent the first guide rail from continuing to move, and the first horizontal movement is stopped.

At this time, the motor 411 continues to drive, referring to fig. 4, the driving part 410 drives the first link 211 to continue moving rightward on the second guide rail, the first link 211 and the second link 212 perform a second horizontal movement, and drives the end of the fourth link 214 to perform a first vertical movement in the guide slot.

As an alternative embodiment, the first horizontal movement is driven by a motor, and the speed of the first horizontal movement comprises a first speed and a second speed; the first speed is greater than the second speed; the first vertical motion is driven by the first horizontal motion; the first speed is greater than the speed of the first vertical movement.

Specifically, at the beginning of the horizontal movement, the motor drive generates a first horizontal movement, and the first speed of the first horizontal movement of the motor drive at this time is set to V1, and the speed of V1 may be higher at this time; when the second stopper moved to the trailing side of the first rail is close to engaging with the first stopper on the second mounting member, the second speed of the first horizontal movement of the motor drive at this time is set to V2, the speed of V2 is slower, and V1> V2.

The state that the second stop at the tail side of the first guide rail is engaged with the first stop on the second mounting component can be judged by a signal provided by a sensor, and can also be judged by combining V1 and the running time of the first guide rail. The speed V2 may be a constant speed or a non-constant speed that gradually slows down.

In the vertical movement, the first vertical movement is driven by the first horizontal movement, the speed of the first vertical movement is set to be V3, V3< V1, and the speeds of V3 and V2 can be the same or different, and can be constant speed or variable speed.

According to the PCR mobile device provided by the embodiment, the impact force of the device when the movement is stopped is reduced by slowing down the movement speed when the movement is stopped, the stability of the device is improved, and the service life of the device is prolonged.

As an alternative embodiment, the first mounting member further comprises a third rail; referring to the internal structure of the first installation part of the PCR displacement apparatus shown in fig. 5, the third guide rail 314 is arranged inside the first installation part and parallel to the guide groove, and the displacement seat 312 is arranged on the third guide rail 314 through the sliding block 315; the first end of the second telescopic member drives the movable base 312 to slide on the third guide rail 314.

The third guide rails can be two, and are respectively connected with the moving seat through the sliding blocks at two ends of the moving seat, so that the moving seat can slide on the third guide rails conveniently.

As an alternative embodiment, the first mounting member further comprises a buffer member, see fig. 5, disposed between the movable base 312 and the sample module; the buffer member includes a guide rod 316 and a spring 317; the guide rod 316 is arranged above the movable seat 312, and the spring 317 is sleeved outside the guide rod 316; the top end of the movable seat 312 is connected to one end of a guide rod 316, and the other end of the guide rod 316 is connected to the sample module.

As an alternative embodiment, the spring has a first and a second amount of deformation in the vertical direction; the second type of variation is used to provide pressure between the heat cap and the sample tube.

The first deformation may be deformation of the spring from an initial state to a state that the spring is compressed by the movable seat until the sample module touches the thermal cover, and the second deformation may be further compression of the spring based on the first deformation for providing a pressure between the sample tube carried by the sample module and the thermal cover.

The PCR mobile device provided by the embodiment realizes that the heat cover forms enough pressure on the sample module by controlling the movement of the sample module, so that the temperature control module in the sample module and the sample tube have good heat transfer efficiency, and meanwhile, the enough pressure also prevents sample reagents from evaporating from the test tube, thereby improving the detection accuracy.

In this embodiment, as shown in fig. 6 (a), when the sample module is located outside the PCR body 100, and the sample is placed on the sample module, the switch button is pressed, and the linear motor drives the driving rod to move in the motor direction through the screw rod. At this moment, because the gravity of the whole device consisting of the two first guide rails, the connecting rod arranged on the first guide rails, the shell of the first installation part and the sample module is larger, when the linear motor drives the driving rod to move towards the motor direction through the screw rod, the driving rod can drive the whole device to slide on the second installation part through the first guide rails, and the horizontal sliding of the whole device towards the motor direction is realized. When slid to a certain position, the second stopper on the rear side of the first rail is engaged with the first stopper on the second mounting part, thereby restricting the horizontal movement of the first rail, and the PCR moving device in section (B) of FIG. 6 is in a state where the horizontal movement is stopped (vertical movement is initial) after the sample is loaded.

At this moment, because linear electric motor continues to operate and drives the actuating lever and remove to the motor direction, and first guide rail is restricted, consequently, the actuating lever can drive first connecting rod, and first connecting rod drives the second connecting rod, and the second connecting rod drives the expansion end of third connecting rod and fourth connecting rod simultaneously and moves for the expansion end contained angle of third connecting rod and fourth connecting rod is bigger and bigger, thereby the other end of fourth connecting rod realizes the ascending motion in vertical direction in the guide slot of first installation component shell.

The movement of the other end of the fourth connecting rod drives the moving seat to move upwards, so that the compression spring generates a first deformation amount to drive the sample module to move upwards in the vertical direction, and when the sample module moves upwards and touches the hot cover, the sample module stops moving; but at this moment, the moving seat continues to move upwards under the driving of the fourth connecting rod driven by the motor, and further the compression spring generates a second deformation, so that the pressure between the heat cover and the sample tube is provided, the pressure can enable the temperature control module in the sample module and the sample tube to have good heat transfer efficiency, meanwhile, the sufficient pressure also prevents the sample reagent from evaporating from the test tube, and the detection accuracy is improved.

When the moving seat continues to move to a certain height, the position sensor is triggered to stop moving, and the vertical movement is stopped, as shown in part (C) of fig. 6, so that the sample module and the optical detection structure are in good alignment.

The embodiment of the invention also provides a PCR instrument, which comprises the PCR mobile device and the sample module in any one of the above embodiments.

The PCR instrument provided by the embodiment of the present application has the same technical features as the PCR moving device provided by the above embodiment, so the same technical problems can be solved, and the same technical effects can be achieved.

Finally, it should be noted that: the above embodiments are only used to illustrate the technical solution of the present invention, and not to limit the same; while the invention has been described in detail and with reference to the foregoing embodiments, it will be understood by those skilled in the art that: the technical solutions described in the foregoing embodiments may still be modified, or some or all of the technical features may be equivalently replaced; and the modifications or the substitutions do not make the essence of the corresponding technical solutions depart from the scope of the technical solutions of the embodiments of the present invention.

Claims

1. A PCR mobile device, comprising: the first mounting component, the first telescopic component and the second telescopic component;

the first side of the first telescopic part is movably connected with the PCR body, and the second side of the first telescopic part bears the first mounting part; the first telescopic part is used for moving the first mounting part from the outside of the PCR body into the PCR body or moving the first mounting part from the inside of the PCR body to the outside of the PCR body;

the first mounting part comprises a movable seat for placing a sample module;

the first end of the second telescopic component is connected with the movable seat, the second end of the second telescopic component is connected with the first telescopic component, and the second telescopic component is used for moving the movable seat upwards or downwards.

2. The PCR mobile apparatus of claim 1, further comprising: the motor is connected with the driving part, and the driving part is connected with the second telescopic part;

the motor is used for driving the driving component, so that the second telescopic component, the first telescopic component and the first mounting component can move.

3. The PCR mobility apparatus of claim 2, wherein the second telescoping member further comprises a third end, the third end of the second telescoping member being movably coupled to the second side of the first telescoping member.

4. The PCR displacement apparatus of claim 2, wherein the first mounting member further comprises a housing having a guide slot disposed thereon; the movable seat is arranged in the shell, and the second telescopic component enables the movable seat to move along the guide groove.

5. The PCR movement apparatus of claim 4, wherein the second telescoping member comprises: the first connecting rod is connected with the first end of the second connecting rod, the second end of the second connecting rod is hinged with one end of the third connecting rod, and meanwhile, the second end of the second connecting rod is hinged with one end of the fourth connecting rod.

6. The PCR moving device of claim 5, wherein an avoiding groove is formed on the second connecting rod, and when the first end of the second telescopic member is located at the bottom end of the guide groove, the avoiding groove is located corresponding to the guide groove.

7. The PCR movement apparatus of claim 5, wherein the PCR body includes a second mounting part; the first telescopic member includes: a first guide rail and a second guide rail;

the second guide rail is fixed on the first guide rail, and the first guide rail is connected with the second mounting component through a sliding block.

8. The PCR moving device of claim 7, wherein the driving part drives the second guiding rail, the first guiding rail and the first mounting part to perform a first horizontal movement relative to the second mounting part through the first connecting rod;

the driving part drives the first connecting rod and the second connecting rod to perform second horizontal movement relative to the first guide rail;

the driving part drives the end part of the fourth connecting rod to perform first vertical movement in the guide groove through second horizontal movement of the first connecting rod and the second connecting rod;

wherein the first horizontal movement and the second horizontal movement do not occur simultaneously, the second horizontal movement is performed after the first horizontal movement is terminated, or the first horizontal movement is performed after the second horizontal movement is terminated.

9. The PCR movement apparatus of claim 7, wherein the first retractable member further comprises a first stopper disposed at one end of the first guide rail; the second mounting component further comprises a second stop, and the position of the first stop corresponds to the position of the second stop.

10. The PCR movement apparatus of claim 8, wherein the first horizontal motion is driven by the motor, and the speed of the first horizontal motion comprises a first speed and a second speed; the first speed is greater than the second speed; the first vertical motion is driven by the first horizontal motion; the first speed is greater than a speed of the first vertical movement.

11. The PCR movement apparatus of claim 8, wherein the first mounting member further comprises a third rail; the third guide rail is arranged on the inner side of the first mounting part and is parallel to the guide groove, and the moving seat is arranged on the third guide rail through a sliding block; and the first end of the second telescopic part drives the movable seat to slide on the third guide rail.

12. The PCR movement apparatus of claim 11, wherein the first mounting member further comprises a buffer member disposed between the movement seat and the sample module;

the buffer component comprises a guide rod and a spring; the guide rod is arranged above the moving seat, and the spring is sleeved outside the guide rod; the top end of the movable seat is connected with one end of the guide rod, and the other end of the guide rod is connected with the sample module.

13. The PCR movement apparatus of claim 12, wherein the spring has a first amount of deformation and a second amount of deformation in a vertical direction; the second type of variation is used to provide pressure between the heat cover and the sample tube.

14. A PCR instrument comprising the PCR moving apparatus and the sample module according to any one of claims 1 to 13.