CN208104392U - A kind of real-time fluorescence quantitative PCR instrument temperature control module lifting device - Google Patents
A kind of real-time fluorescence quantitative PCR instrument temperature control module lifting device Download PDFInfo
- Publication number
- CN208104392U CN208104392U CN201820401573.9U CN201820401573U CN208104392U CN 208104392 U CN208104392 U CN 208104392U CN 201820401573 U CN201820401573 U CN 201820401573U CN 208104392 U CN208104392 U CN 208104392U
- Authority
- CN
- China
- Prior art keywords
- control module
- temperature control
- sphenoid
- guide
- real
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Landscapes
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
Abstract
The utility model relates to a kind of real-time fluorescence quantitative PCR instrument temperature control module lifting devices, including pedestal, two groups of parallel linear guides are provided on pedestal, sphenoid is installed between two groups of linear guides, the side of sphenoid is equipped with the driving device for driving it along linear guide reciprocating movement, the sphenoid two sides parallel with linear guide are respectively provided with an angled straight lines guide rail, sloping slide is installed on each angled straight lines guide rail, the top of sloping slide is equipped with wedge block vertically, the upper end of wedge block is fixedly connected with temperature control module, the lower end of wedge block is fixedly connected with sloping slide.The utility model has the beneficial effects that structure is simple, small volume can steadily drive temperature control module to be moved to heat lid and press hot lid, avoid optical system mobile, guarantee the stability of optical system, improve the accuracy of detection.
Description
Technical field
The utility model relates to technical field of medical equipment, and in particular to a kind of real-time fluorescence quantitative PCR instrument temperature control module
Lifting device.
Background technique
PCR (abbreviation QPCR instrument) is exactly to do the big of In Vitro in external or test tube to specific gene using archaeal dna polymerase
Amount synthesis, substantially it is the chain duplication that specificity is carried out using archaeal dna polymerase.Existing round pcr makes people can be with
Specific nucleic acid fragment is purposefully infinitely expanded in vitro.Its principle is similar to the internal duplication of DNA, only in centrifuge tube
A kind of appropraite condition is provided to the external synthesis of DNA.The appearance of PCR instrument then makes the technology realize automation, so that the skill of PCR
Art is more widely applied, for example, diagnosing hereditary illness, detection clinical samples in pathogen nucleic acid, legal medical expert's sample is lost
It passes and learns identification, and catastrophe in analysis activated oncogene etc. is all widely used.
Traditional PCR instrument is recycled by heating and cooling DNA fragmentation expanding thousands of times, its core is temperature control module.QPCR
Instrument increases optical excitation and detection system on the basis of normal PCR instrument.The temperature control module of major part QPCR instrument currently on the market
Be it is fixed, achieved the purpose that and test tube presses by moving downward for optical module and Re Gai.The shortcomings that this structure is
Volume is larger, and structure is complicated, and optical system will be moved as heat lid is mobile, influences the stability of optical system.
Utility model content
The technical problem to be solved by the utility model is to provide a kind of liftings of real-time fluorescence quantitative PCR instrument temperature control module to fill
It sets, which can steadily drive temperature control module to be moved to heat lid and press hot lid, avoid optical system mobile, guarantee optics
The stability of system improves the accuracy of detection.
The technical solution that the utility model solves above-mentioned technical problem is as follows：
A kind of real-time fluorescence quantitative PCR instrument temperature control module lifting device, including pedestal, be provided on the pedestal two groups it is flat
Capable linear guide is equipped with sphenoid between linear guide described in two groups, and the side of the sphenoid, which is equipped with, drives its edge
The driving device that moves back and forth of the linear guide, the sphenoid two sides parallel with the linear guide are respectively provided with one
Angled straight lines guide rail is equipped with sloping slide on each angled straight lines guide rail, and the top of the sloping slide is equipped with vertically
The upper end of wedge block, the wedge block is fixedly connected with temperature control module, and the lower end of the wedge block is fixed with the sloping slide
Connection.
The utility model has the beneficial effects that：Sphenoid is driven to move back and forth along linear guide by driving device, wedge
Shape block follows sloping slide then to move along angled straight lines guide rail with the movement of sphenoid, temperature control module with wedge block shifting
It moves and is moved up and down along guide rod, be bonded or separate with the heat lid above it to realize；The utility model structure is simple, entirely
The optical system of the hot side of covering is fixed in moving process, increases the stability of optical system, improves the accurate of measurement
Degree.
Based on the above technical solution, the utility model can also do following improvement.
Further, the driving device is lead screw motor, and the lead screw motor is electrically connected with control device, the screw rod electricity
The output end of machine is connect with the side of the sphenoid by feed screw nut.
Beneficial effect using above-mentioned further scheme is the straight line that lead screw motor converts its rotary motion to sphenoid
Movement, while lead screw motor has a self-locking function, lead screw motor by sphenoid push to after corresponding position can self-locked, linearly
The precision of movement is high.
Further, the position that the lead screw motor is connect with the sphenoid is equipped with pressure sensor, the pressure sensing
Device is electrically connected with the control device.
Beneficial effect using above-mentioned further scheme is that lead screw motor pushes the thrust of sphenoid and temperature control module to press
Heat lid pressure correspond to it is proportional, by pressure sensor by lead screw motor push sphenoid pressure send to control fill
It sets, control device receives data and compares and analyzes, when the pressure that temperature control module presses heat lid is more than setting pressure threshold,
Lead screw motor stops working.
Further, linear guide described in every group is made of multiple horizontal sliders, and the upper surface of each horizontal slider is equal
There are multiple balls equipped with uniformly distributed in guide-track groove and the guide-track groove.
Beneficial effect using above-mentioned further scheme is to guide sphenoid mobile by multiple horizontal sliders, horizontal slider
Guide rail in ball can reduce the frictional force between sphenoid and horizontal slider, reduce energy consumption.
Further, be respectively provided with two guide rods on the outside of linear guide described in every group, the bottom of each guide rod with it is described
Pedestal is fixedly connected, and the top of each guide rod is fixedly connected with the corresponding position of hot lid installation base plate, four guide rods
Through the temperature control module and the temperature control module can be moved up and down along the guide rod.
Beneficial effect using above-mentioned further scheme is that temperature control module is moved up and down along four guide rods, and stability increases
Add.
Detailed description of the invention
Fig. 1 is the structural schematic diagram of the utility model propradation；
Fig. 2 is the structural schematic diagram that the utility model declines state；
Fig. 3 is the structural schematic diagram pressed on temperature control module in the utility model；
Fig. 4 is prior art heat lid and the structural schematic diagram that optical system pushes.
In attached drawing, parts list represented by the reference numerals are as follows：
1, pedestal, 2, sphenoid, 3, driving device, 4, angled straight lines guide rail, 5, sloping slide, 6, wedge block, 7, temperature control
Module, 8, pressure sensor, 9, horizontal slider, 10, guide rod, 11, optical system, 12, Re Gai, 13, metal sample pond, 14, sample
Product.
Specific embodiment
The principles of the present invention and feature are described below in conjunction with attached drawing, example is served only for explaining that this is practical
It is novel, it is not intended to limit the scope of the utility model.
As depicted in figs. 1 and 2, the utility model provides a kind of real-time fluorescence quantitative PCR instrument temperature control module lifting device, packet
Pedestal 1 is included, two groups of parallel linear guides are provided on pedestal 1, sphenoid 2, sphenoid 2 are installed between two groups of linear guides
It is to be formed by stacking by two pieces of clapboards, the side of sphenoid 2 is equipped with the driving dress for driving it along linear guide reciprocating movement
Set 3；The two sides parallel with linear guide of sphenoid 2 are respectively provided with an angled straight lines guide rail 4, and two angled straight lines guide rails 4 are installed
On two inclined surface of the clapboard being connect with driving device 3, sloping slide 5 is installed on each angled straight lines guide rail 4, is inclined
Uniformly distributed in the guide rail of angled-lift splits 5 to have multiple balls, ball can reduce rubbing between sloping slide 5 and angled straight lines guide rail 4
Wipe power；The top of sloping slide 5 is equipped with wedge block 6 vertically, and the upper end of wedge block 6 is fixedly connected with temperature control module 7, wedge block 6
Lower end be fixedly connected with sloping slide 5, the upper end of temperature control module 7 is equipped with metal sample pond 13, and metal sample pond 13 includes more
It is a equally distributed to hold sample hole；The surface of temperature control module 7 is equipped with hot lid 12, the lower end of hot lid 12 is uniformly distributed have it is multiple with hold
The optical system 11 of the corresponding light hole in sample hole, hot 12 top of lid acquires optical data by the light hole on hot lid 12.Work
When, driving device 3 drives sphenoid 2 to move back and forth along the linear guide on pedestal 1, and sloping slide 5 is past with sphenoid 2
Multiple movement and moved back and forth along angled straight lines guide rail 4, rising or falling for wedge block 6 is realized, to realize temperature control module 7
It rises or falls, temperature control module 7 is increased continuously until to be fitted closely together with hot lid 12, conversely, the decline of temperature control module 7 and heat
Lid 12 separates.
In the utility model, driving device 3 is lead screw motor, and lead screw motor is electrically connected with control device, lead screw motor
Output end is connect with the side of sphenoid 2 by feed screw nut, and lead screw motor converts its rotary motion in the straight line of sphenoid 2
Movement, while lead screw motor has a self-locking function, lead screw motor by sphenoid 2 push to after corresponding position can self-locked, line
Property movement precision it is high.
Preferably, in the utility model, the position that lead screw motor is connect with sphenoid 2 is equipped with pressure sensor 8, and pressure passes
Sensor 8 is electrically connected with control device.The thrust and temperature control module 7 of lead screw motor promotion sphenoid 2 press the pressure pair of hot lid 12
Answer it is proportional, pressure sensor 8 by the lead screw motor measured push sphenoid 2 thrust send control device to, control
Device receives data and simultaneously compares and analyzes, and the pressure value measured by the pressure sensor 8 is equal to or more than setting pressure threshold
When value, lead screw motor stops working.
In the utility model, every group of linear guide is made of multiple horizontal sliders 9, and each horizontal slider 9 is fixed on pedestal 1
On, the upper surface of each horizontal slider 9 be equipped with guide-track groove and guide-track groove in it is uniformly distributed have multiple balls, sphenoid 2
Two sides are correspondingly provided with guide rail, guide sphenoid 2 mobile by multiple horizontal sliders 9, the ball in the guide rail of horizontal slider 9 can have
Effect reduces the frictional force between sphenoid 2 and horizontal slider 9, reduces energy consumption.In addition to above structure, guide rail be may be provided on pedestal 1,
Horizontal slider 9 is fixedly connected with sphenoid 2, and sphenoid 2 and horizontal slider 9 are moved back and forth along guide rail together.
In the utility model, two guide rods 10, the bottom and bottom of each guide rod 10 are respectively provided on the outside of every group of linear guide
Seat 1 is fixedly connected, and the top of each guide rod 10 and the corresponding position for 12 installation base plate of hot lid being located at right above temperature control module 7 are solid
Fixed connection, hot lid 12 are distinctive heatable lids on QPCR instrument, and when sample 14 detects, hot lid 12 is covered on sample tube,
It can prevent reaction solution from condensing on lid, it is ensured that reaction is not lost as far as possible, and four guide rods 10 run through temperature control module 7 and temperature control
Module 7 can be moved up and down along guide rod 10, and four guide rods 10 can obviously increase stability during temperature control module 7 moves up and down.
The working principle of the utility model is as follows：
As shown in figure 3, sample to be tested 14 is placed in sample drawer when needing test sample 14, push button postposition
Sample drawer moves to the lower section of hot lid 12 from the side of hot lid 12 automatically, so that the light hole one on sample 14 and hot lid 12 is a pair of
It answers, control device controls lead screw motor and rotates forward, and lead screw motor pushes sphenoid 2 to move forward along linear guide；Sphenoid 2 moves
In dynamic process, the sloping slide 5 of 2 two sides of sphenoid moves from the bottom up along corresponding angled straight lines guide rail 4, so that wedge-shaped
Block 6 constantly rises, and is adjacent to sample hole of holding thereon with the undertaking of 14 bottom of sample so that temperature control module 7 be pushed to increase continuously until,
Optical system 11 starts to detect；During temperature control module 7 is adjacent to hot lid 12, pressure sensor 8 pushes away the lead screw motor measured
The thrust of dynamic sphenoid 2 sends control device to, and control device receives data and compares and analyzes, when 8 institute of pressure sensor
When the pressure value measured is equal to or more than setting pressure threshold, lead screw motor stops pushing sphenoid 2.The detection of sample 14 is completed
Afterwards, the reversion of control device control lead screw motor, lead screw motor pull sphenoid 2 to return along linear guide；What sphenoid 2 returned
In the process, the sloping slide 5 of 2 two sides of sphenoid moves from top to bottom along corresponding angled straight lines guide rail 4, so that wedge block 6
Constantly decline, to pull the constantly decline of temperature control module 7 until separating with hot lid 12.
In the utility model, optical system 11 and Re Gai 12 are fixed, press on temperature control module 7, whole process light
The stability of system 11 is good, and detection accuracy is high, and traditional technology is then that optical system 11 and Re Gai 12 are pushed (such as Fig. 4 institute
Show), the stability of optical system 11 is seriously affected, measurement result is caused deviation occur.
The above is only the preferred embodiment of the present invention, is not intended to limit the utility model, all practical at this
Within novel spirit and principle, any modification, equivalent replacement, improvement and so on should be included in the guarantor of the utility model
Within the scope of shield.
Claims (5)
1. a kind of real-time fluorescence quantitative PCR instrument temperature control module lifting device, it is characterised in that：Including pedestal (1), the pedestal
(1) it is provided with two groups of parallel linear guides on, sphenoid (2), the sphenoid are installed between linear guide described in two groups
(2) side, which is equipped with, drives it along the driving device (3) that the linear guide moves back and forth, the sphenoid (2) and institute
It states the parallel two sides of linear guide and is respectively provided with an angled straight lines guide rail (4), be equipped on each angled straight lines guide rail (4)
The top of sloping slide (5), the sloping slide (5) is equipped with wedge block (6) vertically, the upper end of the wedge block (6) and temperature control
Module (7) is fixedly connected, and the lower end of the wedge block (6) is fixedly connected with the sloping slide (5).
2. a kind of real-time fluorescence quantitative PCR instrument temperature control module lifting device according to claim 1, it is characterised in that：Institute
Stating driving device (3) is lead screw motor, and the lead screw motor is electrically connected with control device, the output end of the lead screw motor and institute
The side for stating sphenoid (2) is connected by feed screw nut.
3. a kind of real-time fluorescence quantitative PCR instrument temperature control module lifting device according to claim 2, it is characterised in that：Institute
State position that lead screw motor is connect with the sphenoid (2) and be equipped with pressure sensor (8), the pressure sensor (8) with it is described
Control device electrical connection.
4. a kind of real-time fluorescence quantitative PCR instrument temperature control module lifting device according to claim 1, it is characterised in that：Often
The group linear guide is made of multiple horizontal sliders (9), the upper surface of each horizontal slider (9) be equipped with guide-track groove and
It is uniformly distributed in the guide-track groove to have multiple balls.
5. a kind of real-time fluorescence quantitative PCR instrument temperature control module lifting device according to claim 1-4, feature
It is：Two guide rods (10), the bottom and the bottom of each guide rod (10) are respectively provided on the outside of linear guide described in every group
Seat (1) is fixedly connected, and the top of each guide rod (10) is fixedly connected with the corresponding position of heat lid (12) installation base plate, and four
The guide rod (10) is through the temperature control module (7) and the temperature control module (7) can be moved up and down along the guide rod (10).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201820401573.9U CN208104392U (en) | 2018-03-23 | 2018-03-23 | A kind of real-time fluorescence quantitative PCR instrument temperature control module lifting device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201820401573.9U CN208104392U (en) | 2018-03-23 | 2018-03-23 | A kind of real-time fluorescence quantitative PCR instrument temperature control module lifting device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN208104392U true CN208104392U (en) | 2018-11-16 |
Family
ID=64122484
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201820401573.9U Active CN208104392U (en) | 2018-03-23 | 2018-03-23 | A kind of real-time fluorescence quantitative PCR instrument temperature control module lifting device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN208104392U (en) |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109806923A (en) * | 2019-01-29 | 2019-05-28 | 莫纳（苏州）生物科技有限公司 | A kind of PCR pedestal and PCR instrument |
| CN110577892A (en) * | 2019-08-09 | 2019-12-17 | 苏州百源基因技术有限公司 | Side-open-cover PCR instrument |
| CN110628604A (en) * | 2019-09-11 | 2019-12-31 | 莫纳（苏州）生物科技有限公司 | PCR module movable fluorescent quantitative PCR instrument, PCR instrument set and method |
| CN111500410A (en) * | 2020-04-26 | 2020-08-07 | 杭州博日科技有限公司 | PCR mobile device and PCR appearance |
| CN114181823A (en) * | 2022-02-16 | 2022-03-15 | 苏州雅睿生物技术股份有限公司 | PCR instrument with multiple temperature control modules and asynchronous optional channels and detection method thereof |
| CN114214186A (en) * | 2021-12-10 | 2022-03-22 | 武汉承启医学科技有限公司 | Device for rapidly detecting animal pathogens by using constant-temperature amplification technology |
-
2018
- 2018-03-23 CN CN201820401573.9U patent/CN208104392U/en active Active
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109806923A (en) * | 2019-01-29 | 2019-05-28 | 莫纳（苏州）生物科技有限公司 | A kind of PCR pedestal and PCR instrument |
| CN110577892A (en) * | 2019-08-09 | 2019-12-17 | 苏州百源基因技术有限公司 | Side-open-cover PCR instrument |
| CN110628604A (en) * | 2019-09-11 | 2019-12-31 | 莫纳（苏州）生物科技有限公司 | PCR module movable fluorescent quantitative PCR instrument, PCR instrument set and method |
| CN111500410A (en) * | 2020-04-26 | 2020-08-07 | 杭州博日科技有限公司 | PCR mobile device and PCR appearance |
| CN111500410B (en) * | 2020-04-26 | 2024-02-27 | 杭州博日科技股份有限公司 | PCR mobile device and PCR appearance |
| CN114214186A (en) * | 2021-12-10 | 2022-03-22 | 武汉承启医学科技有限公司 | Device for rapidly detecting animal pathogens by using constant-temperature amplification technology |
| CN114214186B (en) * | 2021-12-10 | 2023-08-25 | 武汉承启医学科技有限公司 | Device for rapidly detecting animal pathogens by using isothermal amplification technology |
| CN114181823A (en) * | 2022-02-16 | 2022-03-15 | 苏州雅睿生物技术股份有限公司 | PCR instrument with multiple temperature control modules and asynchronous optional channels and detection method thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN208104392U (en) | A kind of real-time fluorescence quantitative PCR instrument temperature control module lifting device | |
| CN106434330B (en) | A kind of absolute quantitation digital nucleic acid analysis system based on efficient drop microreactor | |
| CN105403467B (en) | It splits to draw in testing machine and high temperature in concrete material high temperature and splits drawing test method | |
| KR20220041207A (en) | Ultrafast PCR reaction detection system and detection method | |
| CN205229171U (en) | Sample test tube type automatic identification equipment | |
| CN110564610B (en) | Double-temperature-zone PCR amplification device | |
| CN207204137U (en) | A kind of spacing adjusting device | |
| CN105044336A (en) | Multi-channel detecting instrument | |
| CN108319532B (en) | Automatic test line and automatic test method for notebook computer | |
| CN111272805A (en) | System and method for rapidly detecting heat conductivity coefficient of vacuum heat insulation plate | |
| CN110749303A (en) | Forged part quality detection device | |
| CN203117200U (en) | Cement setting time determinator | |
| CN208471885U (en) | Lift induction heating apparatus and glass bending molding machine | |
| CN107384777A (en) | A kind of multi-temperature zone gene amplification device | |
| CN101221107B (en) | Automatic sampling thermal state strain compression tester and its measuring method | |
| CN206975072U (en) | A kind of resistance testing device | |
| CN210945609U (en) | Extremely fast PCR reaction detection device | |
| CN204964534U (en) | Automatic sample device for detecting system | |
| CN107192625B (en) | Heat engine fatigue testing method and heat engine fatigue testing machine using same | |
| CN205262877U (en) | Split in concrete material high temperature and draw testing machine | |
| CN210528943U (en) | Linear scanning PCR instrument | |
| CN213813319U (en) | Automatic sample adding device for fluorescent quantitative PCR | |
| CN210604202U (en) | Continuous sample injection high-temperature bending resistance tester | |
| CN206132790U (en) | Sample holder bears device with unilateral mechanism that opens and shuts | |
| CN114181819B (en) | PCR detection device |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant |