CN111494294A

CN111494294A - Bee pollen mask and preparation method thereof

Info

Publication number: CN111494294A
Application number: CN202010487584.5A
Authority: CN
Inventors: 孙建安; 崔保金; 毛相朝; 崔子琛
Original assignee: Ocean University of China
Current assignee: Ocean University of China
Priority date: 2020-06-02
Filing date: 2020-06-02
Publication date: 2020-08-07
Anticipated expiration: 2040-06-02
Also published as: CN111494294B

Abstract

The invention belongs to the field of facial masks, and discloses a bee pollen facial mask and a preparation method thereof. The bee pollen facial mask comprises bee pollen enzymolysis liquid and nutrient solution, wherein the nutrient solution comprises sea cucumber polysaccharide extract, ginseng extract, tuckahoe extract, pearl powder, aloe, honey and hyaluronic acid, and the preparation method comprises the following steps: (1) adding water into bee pollen, mixing, breaking cell wall, cooling to room temperature in boiling water bath, and adding protease for enzymolysis; (2) centrifuging the bee pollen enzymolysis liquid, collecting supernatant, mixing with the nutrient solution, and adding into facial mask paper. The obtained wall-broken bee pollen has high yield and good quality, and the solution of the wall-broken bee pollen after enzymolysis is uniform, has the special fragrance of bee pollen, and can be conveniently used in various fields of daily necessities, cosmetics, functional foods, health products and the like.

Description

Bee pollen mask and preparation method thereof

Technical Field

The invention relates to the field of masks, and particularly relates to a bee pollen mask and a preparation method thereof.

Background

Bee pollen is from nature, is pollen grains collected by bees from stamens of flowering plants (honey source plants and pollen source plants), and is mixed with special glandular secretions (nectar and saliva) to form an irregular oblate. Bee pollen has natural health care function and medical and beauty values, is recognized by more and more people, is a high-protein low-fat nutritional health food, is known as 'omnipotent nutritional food', 'concentrated natural medicine storehouse', 'cosmetic for oral administration' and the like, and is treasure in natural food of human beings. With the rapid development of bee breeding industry, the annual output of bee pollen in China reaches about 1000 tons.

At present, most of bee pollen products sold at home and abroad are directly eaten, and related bee pollen processing products are very few. However, research shows that the bee pollen has hard, acid-resistant and alkali-resistant sporopouenin outer wall, so that the nutrient substances in the pollen cannot be digested and absorbed after entering the human body. The bee pollen wall breaking mode is also researched at home and abroad, but the wall breaking mode mainly comprises a biological method, a physical method and a mechanical method at present, each wall breaking method has the defects, and the wall breaking rate of a single physical method is not high generally. A great deal of research at home and abroad proves that the protease enzymolysis is beneficial to the generation of functional active peptide, but the related research on the enzymolysis of the bee pollen crushing liquid and the separation and identification of active ingredients do not exist, the high-value utilization of bee pollen products cannot be realized, and the field of the bee pollen enzymolysis liquid products is blank. Relevant researches prove that the bee pollen has tyrosinase activity inhibition effect, but the research on the properties of the enzymolysis liquid of the wall-broken bee pollen is not available.

Disclosure of Invention

The invention aims to overcome the defects of the background technology and provides a bee pollen mask and a preparation method thereof, the invention proves that enzymolysis is beneficial to the release of active substances in bee pollen, the tyrosinase inhibitory activity is improved, the defects of research on a bee pollen wall breaking method and high-efficiency utilization and functional research of bee pollen crushing liquid at the present stage are overcome, and the extraction comprehensive utilization of the bee pollen active substances (protein, polysaccharide and phenolic substances) with high efficiency, high quality, low cost and no pollution is realized. The bee pollen facial mask prepared by the method has the effects of whitening, fading spots, brightening skin, replenishing water, moisturizing and the like, has the peculiar fragrance of bee pollen, is prepared from pure natural raw materials, effectively whitens facial skin, and does not cause harm to human bodies and skin.

In order to achieve the purpose of the invention, the bee pollen facial mask comprises bee pollen enzymolysis liquid and nutrient solution, wherein the nutrient solution comprises sea cucumber polysaccharide extract, ginseng extract, tuckahoe extract, pearl powder, aloe, honey and hyaluronic acid.

Further, the volume ratio of the nutrient solution to the bee pollen enzymolysis solution is 1: (0.9-1.1).

Further, the nutrient solution comprises 1-2 parts of sea cucumber polysaccharide extract, 1-2 parts of ginseng extract, 5-12 parts of poria cocos extract, 1-3 parts of pearl powder, 8-12 parts of aloe, 3-5 parts of honey and 0.01-0.05 part of hyaluronic acid.

Further, the bee pollen enzymolysis liquid is obtained by adding protease into the wall-broken bee pollen liquid for enzymolysis.

Further, the wall-breaking bee pollen liquid is prepared by adding water into bee pollen, mixing, breaking wall with high-pressure cell wall-breaking instrument, boiling in water bath for 8-13min, and cooling to room temperature.

Further, the ratio of the bee pollen to the water is 1: (5-10).

Further, the protease is one or more of papain, neutral protease and flavourzyme.

Further, the enzymolysis temperature is 40-70 ℃, and the enzymolysis time is 0.5-6 hours.

In another aspect, the invention further provides a preparation method of the bee pollen mask, which comprises the following steps:

(1) adding water into bee pollen, mixing, breaking cell wall with high pressure cell wall breaking instrument, boiling water bath for 8-13min, cooling to room temperature, adding protease, and performing enzymolysis to obtain bee pollen enzymolysis solution, wherein the ratio of bee pollen to water is 1: (5-10), the protease is one or more of papain, neutral protease and flavourzyme, the enzymolysis temperature is 40-70 ℃, and the enzymolysis time is 0.5-6 hours;

(2) centrifuging the bee pollen enzymolysis liquid, collecting supernatant, mixing with the nutrient solution, and adding into facial mask paper.

Further, the wall breaking treatment time of the bee pollen after being uniformly mixed with water is 0.5-2min, and the pressure is 600-900 Bar.

Compared with the prior art, the invention has the following advantages:

(1) the method uses the high-pressure cell disruptor to break the cell wall of the bee pollen, has the advantages of low energy consumption, high cell-wall breaking rate, small nutrient loss and the like, and can ensure the product quality and reduce the production cost by breaking the cell wall of the bee pollen by using the method;

(2) the enzymolysis of the wall-broken bee pollen is beneficial to the release of bee pollen active substances, the enzymolysis process can obviously improve the tyrosinase inhibitory activity and enhance the whitening effect, and the technology does not need to combine other wall-breaking means, thereby reducing the production cost;

(3) the obtained wall-broken bee pollen has high yield and good quality, and the solution of the wall-broken bee pollen after enzymolysis is uniform, has the special fragrance of bee pollen, and can be conveniently used in various fields of daily necessities, cosmetics, functional foods, health products and the like.

Detailed Description

In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is further described in detail with reference to the following embodiments. Additional aspects and advantages of the invention will be set forth in part in the description which follows and, in part, will be obvious from the description, or may be learned by practice of the invention. It is to be understood that the following description is only illustrative of the present invention and is not to be construed as limiting the present invention.

The terms "comprises," "comprising," "includes," "including," "has," "having," "contains," "containing," or any other variation thereof, as used herein, are intended to cover a non-exclusive inclusion. For example, a composition, process, method, article, or apparatus that comprises a list of elements is not necessarily limited to only those elements but may include other elements not expressly listed or inherent to such composition, process, method, article, or apparatus.

When an amount, concentration, or other value or parameter is expressed as a range, preferred range, or as a range of upper preferable values and lower preferable values, this is to be understood as specifically disclosing all ranges formed from any pair of any upper range limit or preferred value and any lower range limit or preferred value, regardless of whether ranges are separately disclosed. For example, when a range of "1 to 5" is disclosed, the described range should be interpreted to include the ranges "1 to 4", "1 to 3", "1 to 2 and 4 to 5", "1 to 3 and 5", and the like. When a range of values is described herein, unless otherwise stated, the range is intended to include the endpoints thereof and all integers and fractions within the range.

The technical features of the embodiments of the present invention may be combined with each other as long as they do not conflict with each other.

The nutrient solution contains ginseng, tuckahoe, aloe, pearl powder, sea cucumber polysaccharide and honey. Aloe in cosmetics has effects of nourishing, moisturizing, diminishing inflammation and healing wound, and Margarita powder has effects of resolving macula and whitening skin. In the invention, aloe is squeezed to obtain supernatant, and the supernatant is added with pearl powder and hyaluronic acid when preparing nutrient solution.

Because the ginseng contains the ginsenoside, the blood circulation can be promoted, the skin aging and wrinkle generation can be prevented, the nutrition supply of the skin can be increased, the effects of tonifying qi, beautifying and activating blood can be achieved, the immunity function can be improved, and the skin can be prevented from being harmfully stimulated by the external environment. Poria has effects in removing speckle, whitening skin, moistening skin, enhancing immunity, and dilating blood vessel. The preparation method of the ginseng extract and the tuckahoe extract comprises the following steps: adding pure water with the same volume into Ginseng radix or Poria, boiling, decocting, pouring out the liquid when the volume of the liquid is reduced to one half of the original volume, adding pure water with the same volume, decocting, repeating the above steps twice, and mixing the obtained liquids to obtain extractive solution.

The sea cucumber acidic polysaccharide component contained in the sea cucumber has a protective effect on intracellular and extracellular bound water. The preparation method of the sea cucumber polysaccharide extract comprises the following steps: directly dissolving the sea cucumber polysaccharide powder in distilled water to prepare a 10% solution to obtain a sea cucumber polysaccharide extracting solution.

Example 1

Adding water into bee pollen according to the material-liquid ratio of 1: 7, homogenizing and mixing uniformly, performing wall breaking treatment by using a high-pressure cell disruptor with the pressure of 800Bar and the flow of 12 liters/hour, centrifuging the wall breaking bee pollen liquid obtained by using the method of the invention at 8000rpm, taking supernate, adding nutrient solution (the volume ratio of the nutrient solution to the bee pollen enzymolysis liquid is 1: 1, and the nutrient solution comprises 2 parts of sea cucumber polysaccharide extract, 2 parts of ginseng extract, 12 parts of tuckahoe extract, 3 parts of pearl powder, 12 parts of aloe, 5 parts of honey mask and 0.05 part of hyaluronic acid) to prepare the bee pollen nutrient solution, and measuring the tyrosinase activity inhibition effect, wherein the system is that 450 microliters of supernate reacts with 450 microliters of 0.05mg/100ml substrate L-tyrosine and 100 microliters of 1ku/ml tyrosine, and the obtained bee pollen mask nutrient solution supernate has the tyrosinase activity inhibition rate of 30.2%.

Example 2

Adding water into bee pollen according to the material-liquid ratio of 1: 7, homogenizing and mixing uniformly, performing wall breaking treatment by using a high-pressure cell disruptor under the pressure of 800Bar and the flow rate of 12 liters/hour, after wall breaking, boiling in a boiling water bath for 10min, cooling to room temperature, adjusting the pH to 5.5, adding 1% of solid neutral protease for enzymolysis, performing enzymolysis for 3 hours, centrifuging at 8000 turns, taking supernate, adding nutrient solution (the volume ratio of the nutrient solution to the bee pollen enzymolysis solution is 1: 1, and the nutrient solution comprises 2 parts of sea cucumber polysaccharide extract, 2 parts of ginseng extract, 12 parts of poria cocos extract, 3 parts of pearl powder, 12 parts of aloe, 5 parts of honey and 0.05 part of transparent substance) to prepare the bee pollen mask nutrient solution in microliter, and measuring the tyrosinase activity inhibition effect, wherein the system is that 450 microliter of supernate reacts with 450.05 mg/100ml of substrate L-tyrosine and 100 microliter of 1ku/ml of tyrosine, and the tyrosinase activity inhibition rate of the obtained bee pollen mask nutrient solution in supernate is 98.7%.

Example 3

Adding water into bee pollen according to a material-liquid ratio of 1: 5, homogenizing and mixing uniformly, performing wall breaking treatment by using a high-pressure cell disruptor under the pressure of 600Bar and the flow rate of 12L/h, after wall breaking, boiling in a boiling water bath for 10min, cooling to room temperature, adding papain with the solid amount of 2% for enzymolysis, performing enzymolysis for 5 h, centrifuging at 8000 turns, taking supernate, adding nutrient solution (the volume ratio of the nutrient solution to the bee pollen enzymolysis solution is 1: 1, and the nutrient solution comprises 1 part of sea cucumber polysaccharide extract, 1 part of ginseng extract, 5 parts of poria cocos extract, 1 part of pearl powder, 8 parts of aloe, 3 parts of honey and 0.01 part of hyaluronic acid) to prepare the bee pollen mask nutrient solution, measuring the activity inhibition of tyrosinase, wherein the system is that the activity of the supernate is 450 microliters, the substrate L-tyrosine is 0.05mg/100ml and the tyrosine of 1ku/ml is 100 microliters, and the activity inhibition rate of the supernate of the bee pollen mask nutrient solution is 97.9%.

The facial mask obtained in the embodiments 1 to 3 has good skin feel, uniform appearance, good heat resistance and cold resistance, no separation and layering condition after centrifugal test, good stability, no irritation after use, comfortable feeling, no adverse reaction, and sensory index and physicochemical index both meeting the industrial standard. Through determination, the prepared mask product has no heavy metal exceeding condition and accords with the regulation of cosmetic hygiene standard.

It will be understood by those skilled in the art that the foregoing is only exemplary of the present invention, and is not intended to limit the invention, which is intended to cover any variations, equivalents, or improvements therein, which fall within the spirit and scope of the invention.

Claims

1. A bee pollen facial mask is characterized by comprising bee pollen enzymolysis liquid and nutrient solution, wherein the nutrient solution comprises sea cucumber polysaccharide extracting solution, ginseng extracting solution, tuckahoe extracting solution, pearl powder, aloe, honey and hyaluronic acid.

2. The bee pollen mask as claimed in claim 1, wherein the volume ratio of the nutrient solution to the bee pollen enzymolysis solution is 1: (0.9-1.1).

3. The bee pollen mask as claimed in claim 1, wherein the nutrient solution comprises 1-2 parts of sea cucumber polysaccharide extract, 1-2 parts of ginseng extract, 5-12 parts of poria cocos extract, 1-3 parts of pearl powder, 8-12 parts of aloe, 3-5 parts of honey and 0.01-0.05 part of hyaluronic acid.

4. The bee pollen mask as claimed in claim 1, wherein the bee pollen enzymolysis solution is obtained by adding protease into wall-broken bee pollen solution for enzymolysis.

5. The bee pollen mask as claimed in claim 1, wherein the bee pollen solution is prepared by adding water into bee pollen, mixing, breaking cell wall with high pressure cell wall breaking instrument, boiling in water bath for 8-13min, and cooling to room temperature.

6. The bee pollen mask as claimed in claim 1, wherein the ratio of bee pollen to water is 1: (5-10).

7. The bee pollen mask as claimed in claim 1, wherein the protease is one or more of papain, neutral protease and flavourzyme.

8. The bee pollen mask as claimed in claim 1, wherein the enzymolysis temperature is 40-70 ℃ and the enzymolysis time is 0.5-6 hours.

9. A method for preparing a bee pollen mask as claimed in any one of claims 1 to 8, which comprises the steps of:

10. The method for preparing a bee pollen facial mask as claimed in claim 9, wherein the wall breaking treatment time of the bee pollen after being mixed with water is 0.5-2min, and the pressure is 600-900 Bar.