CN111474360A - Dry-type fluorescence immunoassay appearance - Google Patents
Dry-type fluorescence immunoassay appearance Download PDFInfo
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- CN111474360A CN111474360A CN202010418768.6A CN202010418768A CN111474360A CN 111474360 A CN111474360 A CN 111474360A CN 202010418768 A CN202010418768 A CN 202010418768A CN 111474360 A CN111474360 A CN 111474360A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
- G01N33/582—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/558—Immunoassay; Biospecific binding assay; Materials therefor using diffusion or migration of antigen or antibody
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
- G01N33/585—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with a particulate label, e.g. coloured latex
Abstract
The invention discloses a dry-type fluorescence immunoassay instrument, which comprises a transportation mechanism, an incubation mechanism, a lighting mechanism, a reagent card box storage mechanism, a sample adding mechanism, a sample frame mechanism, a suction head mechanism and a movable frame, wherein the transportation mechanism, the incubation mechanism, the lighting mechanism, the reagent card box storage mechanism, the sample adding mechanism, the sample frame mechanism, the suction head mechanism and the movable frame are integrated on a bottom; the transportation mechanism is used for transporting the reagent card, it is used for awaiting measuring the reagent card and carries out the preliminary treatment with sample liquid to incubate the mechanism, application of sample mechanism is used for adding sample liquid to the awaiting measuring reagent card, suction head mechanism is used for the ration to absorb sample liquid, sample frame mechanism is used for depositing the test tube, the in-tube storage has sample liquid, daylighting mechanism is used for carrying out optical information data acquisition and output to the reagent card after the preliminary treatment, the adjustable shelf is used for realizing application of sample mechanism's horizontal and longitudinal movement. The invention solves the problem that the prior fluorescence immunoassay analyzer needs a large number of complicated manual steps for auxiliary detection.
Description
Technical Field
The invention relates to the field of medical instruments, in particular to a dry type fluorescence immunoassay instrument.
Background
Brief introduction to fluorescence immunization: the fluorescence immunoassay (also known as fluorescent antibody technology) is the earliest developed one in the labeled immunoassay, and is a technology established on the basis of immunology, biochemistry and microscope technology, and a dry immunofluorescence analyzer: the sample solution dropped on one end of the membrane is moved to the other end by the capillary action of the membrane (lateral flow based on chromatography), the analyte is combined with a receptor (antigen or antibody) fixed on a certain area on the membrane in the moving process and is subjected to solid phase, irrelevant substances are separated by crossing the area, and then the test result is judged by detecting a marker (qualitative, semi-quantitative and quantitative).
The existing fluorescence immunoassay analyzer needs a large amount of complicated manual steps for auxiliary detection.
Disclosure of Invention
The invention aims to provide a dry type fluorescence immunoassay instrument, which solves the problem that the conventional fluorescence immunoassay instrument needs a large number of complicated manual steps for auxiliary detection.
The invention is realized by the following technical scheme:
a dry-type fluorescence immunoassay instrument comprises a transportation mechanism, an incubation mechanism, a lighting mechanism, a reagent card box storage mechanism, a sample adding mechanism, a sample frame mechanism, a suction head mechanism and a movable frame which are integrated on a bottom frame;
the transportation mechanism is used for transporting the reagent card, it is used for awaiting measuring the reagent card and carries out the preliminary treatment with sample liquid to incubate the mechanism, application of sample mechanism is used for adding sample liquid to the awaiting measuring reagent card, suction head mechanism is used for the ration to absorb sample liquid, sample frame mechanism is used for depositing the test tube, the in-tube storage has sample liquid, daylighting mechanism is used for carrying out optical information data acquisition and output to the reagent card after the preliminary treatment, the adjustable shelf is used for realizing application of sample mechanism's horizontal and longitudinal movement.
The sample adding mechanism, the lighting mechanism and the movable frame are all the prior art, and the working principle of the lighting mechanism is as follows: the ultraviolet ray of specific wavelength is launched to reagent card detection area by the light source, arouses the detection, the adjustable shelf includes 2 parallel arrangement's horizontal slide rail, is provided with vertical slide rail on 2 horizontal slide rail, vertical slide rail can move in horizontal slide rail at motor drive, be provided with the sliding block on the vertical slide rail, the sliding block can move in vertical slide rail at motor drive, the application of sample device sets up on the sliding block.
The working principle of the invention is as follows:
the reagent card is stacked in the reagent card box, the reagent card box is placed in the reagent card box storage mechanism, the single-piece reagent card is taken out by the transportation mechanism, an aluminum foil film on the reagent card box is punctured, the reagent card is moved and transported to the incubation mechanism, the sample liquid is quantitatively absorbed by the suction head mechanism through the disposable suction head, the sample liquid in the disposable suction head is dropwise added on the reagent card on the incubation mechanism by the sample adding mechanism, the reagent card is subjected to heating treatment by the incubation mechanism, and the reagent card subjected to heating treatment is transported to the lighting mechanism through the transportation mechanism to be subjected to optical information data acquisition and output.
According to the invention, different functional modules are integrated on the bottom frame, so that automatic sample adding, pretreatment and optical information data acquisition and output of the reagent card can be realized on the same bottom frame, manual operation steps are greatly reduced, quantitative sample liquid absorption is realized through the suction head mechanism, and the sample capacity is accurately controlled.
Meanwhile, the invention has the advantages of small volume and simple structure.
The invention adopts a fluorescent microsphere chromatography mode, can detect and analyze a sample for multiple times, and analyzes the superposed data; at least 30 sample volumes per hour can be detected.
Further, the transportation mechanism comprises a bottom guide rail and a lateral guide rail which is arranged above the bottom guide rail and the sliding direction of which is vertical to the sliding direction of the bottom guide rail, and further comprises a shifting fork mechanism which is arranged on the lateral guide rail and can slide along the sliding direction of the lateral guide rail, a pricking mechanism which is fixedly arranged at one end of the lateral guide rail and used for pricking an aluminum foil film on the upper surface of the reagent card, and a scanner a which is arranged above the pricking mechanism and used for scanning a two-dimensional code on the reagent card.
Specifically, the lateral guide rail can move on the bottom guide rail, so that the transportation mechanism is transported to the incubation mechanism, and the shifting fork mechanism and the puncturing mechanism are arranged on the lateral guide rail in a sliding manner, so that the pretreated reagent card is transported to the lighting mechanism; the reagent card dropwise added with the stock solution to be detected is not manually placed into the corresponding detection mechanism, so that time and labor are saved.
Furthermore, a transmission device a for driving the lateral guide rail to slide on the bottom rail is arranged on the bottom rail, the transmission device a comprises a driving motor a arranged at one end of the bottom rail in the sliding direction and an idler wheel assembly a arranged at the other end of the bottom rail in the sliding direction, and an output shaft of the driving motor a is connected with the idler wheel assembly a through a belt a.
Furthermore, a transmission device b used for driving the shifting fork mechanism to slide on the lateral guide rail is arranged on the lateral guide rail, the transmission device b comprises a driving motor b arranged at one end of the lateral guide rail in the sliding direction and an idler wheel assembly b arranged at the other end of the lateral guide rail in the sliding direction, and an output shaft of the driving motor b is connected with the idler wheel assembly b through a belt b.
The shifting fork mechanism can move along the bottom guide rail and the lateral guide rail under the driving of the driving device a and the driving device b, so that each point on the conveying device can move, and reagent cards can be conveyed to each functional mechanism.
Further, stab the mechanism including the reagent card support that is used for fixed reagent card, set up and be used for stabbing the pole of stabbing of reagent card upper surface aluminium foil membrane and be used for driving the elevating gear a that stabs the pole and reciprocate above the reagent card support, be provided with the shell fragment that is used for fixed reagent card on the reagent card support.
Furthermore, the shifting fork mechanism comprises a lifting device b and a shifting fork rod which is arranged on the lifting device and is used for being matched with a slotted hole at the lower end of the reagent card.
Further, incubate the mechanism including hatching the cardboard, be provided with a plurality of incubation draw-in grooves that are used for fixed reagent card on hatching the cardboard.
Specifically, the heating element on the incubation mechanism heats the incubation groove, and the temperature of the placed reagent card is processed, so that the subsequent lighting mechanism can read optical information smoothly.
Preferably, the incubation mechanism is further provided with a temperature sensor and a heating device, the temperature sensor is used for collecting temperature information, the temperature is effectively controlled by the control system to heat the temperature, the put reagent card is subjected to temperature processing, and the subsequent lighting mechanism is guaranteed to smoothly read the optical information.
Further, reagent card box storage mechanism is including the reagent card box that is used for stacking a plurality of reagent cards, the reagent card box rack that is used for depositing a plurality of reagent card boxes and sets up the abandonment reagent card collection box in reagent card box rack below, be provided with the fixed draw-in groove that is used for fixed reagent card box on the reagent card box rack and be used for the reagent card direction that has detected and throw into the abandonment in the reagent card collection box and withdraw from).
The reagent card adopts a box-type packaging mode, and meanwhile, the formula reagent is packaged in the reagent card, so that the reagent card is convenient to store and use, and the influence of dust and strong illumination on the traditional storage on the detection effect is avoided. Meanwhile, the reagent card after detection is stored for a short time, and cannot be completely exposed in a detection clamping groove of traditional equipment, so that the detection precision is prevented from being influenced by cross contact.
The invention adopts a multi-card packaging mode, and simultaneously adopts a transport mechanism to automatically transfer reagent cards, thereby improving the card-taking efficiency and saving time.
Further, the sample frame mechanism comprises a sample base plate, a sample frame groove for placing the test tube is formed in the sample base plate, a scanner b is arranged on the sample base plate and on the outer side of the sample frame groove, and the scanner b is used for scanning the identification code on the test tube;
further, the suction head mechanism comprises a suction head placing frame and a waste suction head placing box, wherein the suction head placing frame is used for placing disposable suction heads, and the disposable suction heads are arranged on the suction head placing frame in a matrix mode.
The suction head mechanism adopts the disposable suction head, so that the sample pollution and the influence on the detection precision caused by the repeated use of the suction head and the improper cleaning of the suction head are avoided.
Compared with the prior art, the invention has the following advantages and beneficial effects:
1. according to the invention, different functional modules are integrated on the bottom frame, so that automatic sample adding, pretreatment and optical information data acquisition and output of the reagent card can be realized on the same bottom frame, manual operation steps are greatly reduced, quantitative sample liquid absorption is realized through the suction head mechanism, unused disposable suction heads can be automatically recorded and searched, and the sample capacity is accurately controlled.
2. The suction head mechanism adopts the disposable suction head, so that the sample pollution and the influence on the detection precision caused by the repeated use of the suction head and the improper cleaning of the suction head are avoided.
3. The shifting fork mechanism can bring reagent cards to each functional mechanism for corresponding detection operation under the driving of the conveying mechanism, so that the reagent cards dropwise added with stock solution to be detected are not required to be manually placed in the corresponding detection mechanism, time and labor are saved, the shifting fork mechanism can move along the bottom guide rail and the lateral guide rail under the driving of the driving device a and the driving device b, and the shifting of each point on the conveying device is realized, so that the reagent cards are conveyed to each functional mechanism.
4. The scanner a identifies the identification code on the reagent card, locks the reagent card, distinguishes different sample liquids to be detected through the identification code in the incubation mechanism to realize the matching of the sample liquids and the reagent card, the lighting mechanism realizes the matching of the reagent card and a detection result through the identification code, and the scanner a, the lighting mechanism and the incubation mechanism realize the unification of the detection sample liquids, the reagent card and the detection result.
5. The reagent card adopts a box-type packaging mode, and meanwhile, the formula reagent is packaged in the reagent card, so that the reagent card is convenient to store and use, and the influence of dust and strong illumination on the traditional storage on the detection effect is avoided. Meanwhile, the reagent card after detection is stored for a short time, and cannot be completely exposed in a detection clamping groove of traditional equipment, so that the detection precision is prevented from being influenced by cross contact.
6. The invention adopts a multi-card packaging mode, and simultaneously adopts a transport mechanism to automatically transfer reagent cards, thereby improving the card-taking efficiency and saving time.
7. The invention adopts a fluorescent microsphere chromatography mode, can detect and analyze a sample for multiple times, and analyzes the superposed data; at least 30 sample volumes per hour can be detected.
Drawings
The accompanying drawings, which are included to provide a further understanding of the embodiments of the invention and are incorporated in and constitute a part of this application, illustrate embodiment(s) of the invention and together with the description serve to explain the principles of the invention. In the drawings:
FIG. 1 is a first schematic structural diagram of a dry-type fluorescence immunoassay instrument.
Fig. 2 is a schematic structural view of the transport mechanism.
Fig. 3 is a schematic structural diagram of a conveying mechanism driving device a and a driving device b.
FIG. 4 is a schematic diagram of a dry type fluorescence immunoassay instrument.
FIG. 5 is a schematic diagram of a reagent card.
Fig. 6 is a schematic view of the lancing mechanism.
Fig. 7 is a schematic structural view of the fork mechanism.
FIG. 8 is a schematic diagram of the structure of the reagent cartridge storage mechanism.
FIG. 9 is a schematic diagram of a reagent cartridge.
Fig. 10 is a schematic structural view of the movable frame.
FIG. 11 is a schematic structural view of an incubation mechanism.
Fig. 12 is a schematic structural view of the specimen rack mechanism.
Fig. 13 is a schematic view of the structure of the suction head mechanism.
Reference numbers and corresponding part names in the drawings:
1-a transportation mechanism, 2-an incubation mechanism, 3-a lighting mechanism, 4-a reagent card box storage mechanism, 5-a reagent card, 6-a sample adding mechanism, 7-a sample frame mechanism, 8-a sucker mechanism, 9-a test tube, 10-a movable frame, 11-a bottom guide rail, 12-a lateral guide rail, 13-a shifting fork mechanism, 15-a puncturing mechanism, 17-a scanner a, 21-an incubation clamping plate, 41-a reagent card box placing frame, 42-a reagent card box, 43-a reagent card collecting box, 111-a driving motor a, 113-an idler component a, 114-a belt, 121-a driving motor b, 123-an idler component b, 124-a belt b, 131-a shifting fork rod, 132-a lifting device b, 151-a reagent card bracket and 152-a puncturing rod, 153-lifting device a, 211-incubation clamping groove, 71-sample rack groove, 72-scanner b, 81-sucker placing frame, 82-waste sucker placing box, 411-fixed clamping groove and 412-withdrawal groove.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is further described in detail below with reference to examples and accompanying drawings, and the exemplary embodiments and descriptions thereof are only used for explaining the present invention and are not meant to limit the present invention.
Example 1:
as shown in fig. 1-13, a dry-type fluorescence immunoassay instrument comprises a transportation mechanism 1, an incubation mechanism 2, a lighting mechanism 3, a reagent cartridge storage mechanism 4, a sample adding mechanism 6, a sample frame mechanism 7, a suction head mechanism 8 and a movable frame 10, which are integrated on a bottom frame;
the transportation mechanism 1 is used for transporting the reagent card 5, the incubation mechanism 2 is used for pretreating the reagent card 5 to be tested and sample liquid, the sample adding mechanism 6 is used for adding the sample liquid to the reagent card 5 to be tested, the suction head mechanism 8 is used for quantitatively absorbing the sample liquid, the sample rack mechanism 7 is used for storing a test tube 9, the sample liquid is stored in the test tube 9, the lighting mechanism 3 is used for collecting and outputting optical information data of the pretreated reagent card 5, and the movable rack 10 is used for realizing the transverse and longitudinal movement of the sample adding mechanism 6;
the transport mechanism 1 comprises a bottom guide rail 11, a side guide rail 12 which is arranged above the bottom guide rail 11, the sliding direction of the side guide rail 12 is perpendicular to the sliding direction of the bottom guide rail 11, the transport mechanism further comprises a shifting fork mechanism 13 which is arranged on the side guide rail 12 and can slide along the sliding direction of the side guide rail 12, a puncture mechanism 15 which is fixedly arranged at one end of the side guide rail 12 and used for puncturing an aluminum foil film on the upper surface of the reagent card 5, and a scanner a17 which is arranged above the puncture mechanism 15 and used for scanning a two-dimensional code on the reagent card;
the incubation mechanism 2 comprises an incubation clamping plate 21, and a plurality of incubation clamping grooves 211 for fixing the reagent cards 5 are arranged on the incubation clamping plate 21;
the reagent card box storage mechanism 4 comprises a reagent card box 42 for stacking a plurality of reagent cards 5, a reagent card box placing frame 41 for storing a plurality of reagent card boxes 42 and a waste reagent card collecting box 43 arranged below the reagent card box placing frame 41, wherein a fixing card slot 411 for fixing the reagent card box 42 and an exit slot 412 for guiding the detected reagent card 5 to be thrown into the waste reagent card collecting box 43 are arranged on the reagent card box placing frame 41;
the sample frame mechanism 7 comprises a sample bottom plate, a sample frame groove 71 for placing the test tube 9 is arranged on the sample bottom plate, a scanner b72 is arranged on the sample bottom plate at the outer side of the sample frame groove 71, and the scanner b72 is used for scanning an identification code on the test tube 9;
the sucker mechanism 8 comprises a sucker placing frame 81 for placing disposable suckers and a waste sucker placing box 82, and the disposable suckers are arranged on the sucker placing frame 81 in a matrix mode.
The detection method of the embodiment comprises the following steps:
A. stacking the reagent card 5 in the reagent card box 42, and clamping the reagent card box 42 on the reagent card box placing frame 41 through the fixed clamping slot 411;
B. the shifting fork mechanism 13 takes out the reagent card 5 under the driving of the conveying mechanism 1 and adopts the puncturing mechanism 15 to puncture the aluminum foil film on the upper surface of the reagent card 5;
C1. the shifting fork mechanism 13 drives the reagent card 5 to move onto the incubation clamping groove 211 under the driving of the transportation mechanism 1;
C2. a disposable sucker on the sucker mechanism 8 sucks sample liquid quantitatively;
C3. the scanner b72 scans the identification code on the test tube 9 filled with the sample liquid to be detected and uploads the information to the system, the system is matched with the corresponding reagent card 5, the sample adding mechanism 6 moves to the upper part of the suction head mechanism 8 through the movable frame 10 to take out the disposable suction head, and the scanned sample liquid in the test tube 9 is sucked;
C4. then the sample adding mechanism 6 is moved to the position above the reagent card 5 which is matched with the system and is positioned on the incubation clamping groove 211 through the movable frame 10, the sample liquid to be detected is dripped on the reagent card 5, and the reagent in the reagent card 5 is fully reacted with the sample liquid;
C5. the sample adding mechanism 6 is moved to the waste suction head placing box 82 through the movable frame 10 to throw the disposable suction head into the waste suction head placing box;
D. the shifting fork mechanism 13 is driven by the transportation mechanism 1 to transport the reagent card 5 from the incubation clamping groove 211 to the lower part of the lighting mechanism 3, the lighting mechanism 3 collects color information on the reagent card 5 and uploads the color information to a related system, and the shifting fork mechanism 13 transports the reagent card 5 pretreated by the incubation mechanism 2 to the lighting mechanism 3 for data processing;
E. after the lighting mechanism 3 finishes data processing, the transportation mechanism 1 returns to the original position, the shifting fork mechanism 13 is driven by the transportation mechanism 1 to transport the reagent card 5 to the exit groove 412, and the reagent card 5 is guided and thrown into the waste reagent card collecting box 43 through the exit groove 412.
Example 2:
as shown in fig. 1 to 13, this embodiment is based on embodiment 1, a transmission device a for driving the side rail 12 to slide on the bottom rail 11 is disposed on the bottom rail 11, the transmission device a includes a driving motor a111 disposed at one end of the bottom rail 11 in the sliding direction, and an idler pulley assembly a113 disposed at the other end of the bottom rail 11 in the sliding direction, and an output shaft of the driving motor a111 is connected to the idler pulley assembly a113 through a belt a 114; the lateral guide rail 12 is provided with a transmission device b for driving the shifting fork mechanism 13 to slide on the lateral guide rail 12, the transmission device b comprises a driving motor b121 arranged at one end of the lateral guide rail 12 in the sliding direction and an idler wheel assembly b123 arranged at the other end of the lateral guide rail 12 in the sliding direction, and an output shaft of the driving motor b121 is connected with the idler wheel assembly b123 through a belt b 124; the pricking mechanism 15 comprises a reagent card support 151 for fixing a reagent card, a pricking rod 152 arranged above the reagent card support 151 for pricking an aluminum foil film on the upper surface of the reagent card 5, and a lifting device a153 for driving the pricking rod (152) to move up and down, wherein an elastic sheet for fixing the reagent card 5 is arranged on the reagent card support 151; the shifting fork mechanism 13 comprises a lifting device b132 and a shifting fork rod 131 which is arranged on the lifting device 132 and is used for being matched with a slotted hole at the lower end of the reagent card 5.
In this embodiment, the fork mechanism 13 is driven by the transport mechanism 1 to transport the reagent cards 5 in the reagent cartridges 42 to the reagent cartridge holder 151, the poking rod 152 punctures the aluminum foil film on the upper surface of the reagent card 5 downwards under the action of the lifting device a153, and the poking rod device a153 resets the poking rod 152.
The above-mentioned embodiments are intended to illustrate the objects, technical solutions and advantages of the present invention in further detail, and it should be understood that the above-mentioned embodiments are merely exemplary embodiments of the present invention, and are not intended to limit the scope of the present invention, and any modifications, equivalent substitutions, improvements and the like made within the spirit and principle of the present invention should be included in the scope of the present invention.
Claims (10)
1. A dry-type fluorescence immunoassay instrument is characterized by comprising a transportation mechanism (1), an incubation mechanism (2), a lighting mechanism (3), a reagent card box storage mechanism (4), a sample adding mechanism (6), a sample frame mechanism (7), a suction head mechanism (8) and a movable frame (10) which are integrated on a bottom frame;
transport mechanism (1) is used for transporting reagent card (5), it is used for awaiting measuring reagent card (5) and sample liquid to carry out the preliminary treatment to incubate mechanism (2), application of sample mechanism (6) are used for adding sample liquid on awaiting measuring reagent card (5), suction head mechanism (8) are used for the ration to absorb sample liquid, sample frame mechanism (7) are used for depositing test tube (9), the sample liquid has been stored in test tube (9), daylighting mechanism (3) are used for carrying out optical information data collection and output to reagent card (5) after the preliminary treatment, movable rack (10) are used for realizing the horizontal and longitudinal movement of application of sample mechanism (6).
2. The dry type immunofluorescent detector according to claim 1, wherein the transportation mechanism (1) comprises a bottom guide rail (11) and a side guide rail (12) which is arranged above the bottom guide rail (11) and the sliding direction of which is perpendicular to the sliding direction of the bottom guide rail (11), the transportation mechanism further comprises a shifting fork mechanism (13) which is arranged on the side guide rail (12) and can slide along the sliding direction of the side guide rail (12), a puncturing mechanism (15) which is fixedly arranged at one end of the side guide rail (12) and is used for puncturing an aluminum foil film on the upper surface of the reagent card (5), and a scanner a (17) which is arranged above the puncturing mechanism (15) and is used for scanning a two-dimensional code on the reagent card.
3. The dry type immunofluorescent detector according to claim 2, wherein the bottom rail (11) is provided with a transmission device a for driving the side rail (12) to slide on the bottom rail (11), the transmission device a comprises a driving motor a (111) arranged at one end of the bottom rail (11) in the sliding direction and an idler wheel assembly a (113) arranged at the other end of the bottom rail (11) in the sliding direction, and the output shaft of the driving motor a (111) is connected with the idler wheel assembly a (113) through a belt a (114).
4. The dry type immunofluorescent detector according to claim 2, wherein the lateral guide rail (12) is provided with a transmission device b for driving the shifting fork mechanism (13) to slide on the lateral guide rail (12), the transmission device b comprises a driving motor b (121) arranged at one end of the lateral guide rail (12) in the sliding direction and an idler wheel assembly b (123) arranged at the other end of the lateral guide rail (12) in the sliding direction, and an output shaft of the driving motor b (121) is connected with the idler wheel assembly b (123) through a belt b (124).
5. The dry fluoroimmunoassay detector according to claim 2, wherein the puncturing mechanism (15) comprises a reagent card holder (151) for fixing the reagent card, a puncturing rod (152) disposed above the reagent card holder (151) for puncturing the aluminum foil film on the upper surface of the reagent card (5), and a lifting device a (153) for driving the puncturing rod (152) to move up and down, wherein the reagent card holder (151) is provided with a spring for fixing the reagent card (5).
6. The dry type immunofluorescent detector according to claim 2, wherein the fork mechanism (13) comprises a lifting device b (132) and a fork lever (131) provided on the lifting device (132) for engaging with a slot hole at the lower end of the reagent card (5).
7. The dry fluoroimmunoassay detector according to claim 1, wherein the incubation mechanism (2) comprises an incubation card (21), and a plurality of incubation slots (211) for fixing the reagent card (5) are disposed on the incubation card (21).
8. The dry type immunofluorescent detector according to claim 1, wherein the reagent cartridge storage mechanism (4) comprises a reagent cartridge (42) for stacking a plurality of reagent cards (5), a reagent cartridge rack (41) for storing a plurality of reagent cartridges (42), and a waste reagent card collecting box (43) disposed below the reagent cartridge rack (41), wherein a fixing slot (411) for fixing a reagent cartridge (42) and an exit slot (412) for guiding a detected reagent card (5) to be thrown into the waste reagent card collecting box (43) are disposed on the reagent cartridge rack (41).
9. The dry fluoroimmunoassay detector as set forth in claim 1, wherein the sample holder mechanism (7) comprises a sample base plate, the sample base plate is provided with a sample holder slot (71) for holding the test tube (9), the sample base plate is provided with a scanner b (72) outside the sample holder slot (71), and the scanner b (72) is used for scanning the identification code on the test tube (9).
10. The dry fluoroimmunoassay instrument according to claim 1, wherein the tip mechanism (8) comprises a tip placement rack (81) for placing disposable tips and a waste tip placement cassette (82), the disposable tips being arranged in a matrix on the tip placement rack (81).
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112213506A (en) * | 2020-09-04 | 2021-01-12 | 珠海丽珠试剂股份有限公司 | Fluorescence immunoassay appearance |
| CN112557648A (en) * | 2020-12-25 | 2021-03-26 | 重庆康巨全弘生物科技有限公司 | POCT fluorescence immunoassay appearance |
| CN112940920A (en) * | 2021-01-31 | 2021-06-11 | 新羿制造科技（北京）有限公司 | Biochip analyzer |
| CN113376393A (en) * | 2021-07-09 | 2021-09-10 | 广州蓝勃生物科技有限公司 | Full-automatic immunoassay analyzer and detection method |
| CN115825463A (en) * | 2023-02-17 | 2023-03-21 | 世纪亿康（天津）医疗科技发展有限公司 | Blood coagulation detection equipment |
| WO2023216638A1 (en) * | 2022-05-12 | 2023-11-16 | 深圳市帝迈生物技术有限公司 | Testing apparatus for testing coagulation item and reagent card |
| CN117434280A (en) * | 2023-12-21 | 2024-01-23 | 广州誉康医药有限公司 | Reagent card selecting and puncturing system |
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