CN112940920A - Biochip analyzer - Google Patents

Abstract

The invention provides a biochip analyzer, which comprises a rack, wherein a PCR amplification device, a chip feeding device and a sample detection device are arranged on the rack, the PCR amplification device comprises a first shell and a heating platform, the heating platform is provided with a retracting position in the first shell and an extending position outside the first shell, the top of the first shell is provided with a sample placement area to be detected, the biochip analyzer also comprises a transfer device, and the transfer device can transfer a sample on the heating platform at the extending position to the sample placement area to be detected, transfer the sample from the sample placement area to be detected to the sample detection device, and transfer a chip in the chip feeding device to the sample detection device. The transfer device and the functional devices cooperatively act, so that the functions of PCR amplification, sample and biochip feeding, biochip detection and analysis and the like can be realized, the automation degree and the integration degree are high, the analysis and detection efficiency is higher, and the labor cost is greatly reduced.

Description

Biochip analyzer

Technical Field

The invention belongs to the technical field of digital PCR analyzers, and particularly relates to a biochip analyzer.

Background

The digital PCR is the latest quantitative technology, the nucleic acid quantification based on the single molecule PCR method is an absolute quantification method, the method mainly adopts the microfluidic or micro-droplet method in the current analytical chemistry hot research field, a large amount of diluted nucleic acid solution is dispersed into micro-reactors or micro-droplets of a biochip, the number of nucleic acid templates in each reactor is less than or equal to 1, thus after PCR circulation, the reactor with one nucleic acid molecule template can give a fluorescence signal, the reactor without the template has no fluorescence signal, and the nucleic acid concentration of the original solution can be calculated according to the relative proportion and the volume of the reactor. The traditional PCR analyzer has single function, and needs to manually operate a plurality of instruments to complete one-time amplification and detection. With the development of society, the medical industry, the scientific research field and the like put higher requirements on high throughput, automation, high speed, integration and the like of PCR analysis.

Disclosure of Invention

Therefore, the present invention is to provide a biochip analyzer, which can realize the functions of PCR amplification, sample and biochip loading, biochip detection and analysis, etc. by using the transfer device and the plurality of functional devices to cooperate, and has the advantages of high automation degree and integration degree, higher analysis and detection efficiency, and greatly reduced labor cost.

In order to solve the above problems, the present invention provides a biochip analyzer, including a rack, on which a PCR amplification device, a chip loading device, and a sample detection device are mounted, where the PCR amplification device includes a first housing, a heating platform, the heating platform has a retracted position in the first housing and an extended position outside the first housing, and a sample placement area to be detected is configured on the top of the first housing, and the biochip analyzer further includes a transfer device, and the transfer device can transfer a sample on the heating platform at the extended position to the sample placement area to be detected, transfer the sample from the sample placement area to be detected to the sample detection device, and transfer a chip in the chip loading device to the sample detection device.

Preferably, the rack is further provided with a waste recycling bin, and the transfer device can also transfer the chip and/or the sample in the sample detection device into the waste recycling bin.

Preferably, the transfer device includes first guide rail, the second guide rail of erectting in the frame top region, first guide rail sets up two, two first guide rail parallel arrangement each other just is in respectively the length both ends of second guide rail, the second guide rail can be along the length extending direction straight reciprocating motion of first guide rail, sliding connection has material loading hand claw device on the second guide rail.

Preferably, the feeding paw device comprises an electric jaw, the electric jaw is provided with a first translational jaw and a second translational jaw which can move oppositely or back to back, a first clamping block is connected to the first translational jaw, a second clamping block is connected to the second translational jaw, a first calandria clamping part is formed on one side, facing the second clamping block, of the first clamping block, and a second calandria clamping part is formed on one side, facing the first clamping block, of the second clamping block; still construct first chip clamping part on the first grip block, first chip clamping part is in the top of first calandria clamping part, still construct second chip clamping part on the second grip block, the second chip clamping part is in the top of second calandria clamping part.

Preferably, the first tube bank clamping portion comprises a convex block extending towards one side of the second tube bank clamping portion, and the second tube bank clamping portion comprises a groove corresponding to the convex block.

Preferably, be used for the eight calandria subassemblies that link of centre gripping, eight calandria subassemblies include eight link calandria body and are used for fixing the eight seat of arranging of calandria body that links, the mouth of pipe sealing connection of eight link calandria body has the tube cap, the first end of length of arranging the seat be equipped with the row seat recess that the lug corresponds, the length second end of arranging the seat be equipped with the row seat lug that the recess corresponds.

Preferably, the first chip clamping portion comprises a first chip card slot, and the second chip clamping portion comprises a second chip card slot.

Preferably, a positioning pin is further inserted into the first chip clamping groove, and the positioning pin passes through the first chip clamping groove along the vertical direction of the using direction of the feeding paw device so as to horizontally position the microfluidic chip; and/or a positioning pin is also inserted in the second chip clamping groove and penetrates through the second chip clamping groove along the vertical direction of the using direction of the feeding paw device so as to horizontally position the microfluidic chip.

Preferably, the rack is further provided with a liquid storage device, the liquid storage device is provided with a liquid storage shell and a liquid storage box located in the liquid storage shell, and the liquid storage box can be ejected out of the liquid storage shell.

Preferably, the chip feeding device comprises a chip magazine and a chip feeding part located on one side of the chip magazine, the chip magazine is provided with a stacking cavity for stacking chips, a lifting channel is arranged on one side of the chip magazine and is used for communicating the stacking cavity with the outside of the chip magazine, and the chip feeding part comprises a chip supporting claw which extends into the lifting channel.

According to the biochip analyzer, the transfer device and the functional devices such as the PCR amplification device, the chip feeding device and the sample detection device are adopted to cooperatively act, so that the automatic circulation of samples and chips in the PCR amplification, sample and biochip feeding, and biochip detection and analysis processes is realized, the automation degree and the integration degree are high, the analysis and detection efficiency is higher, and the labor cost is greatly reduced.

Drawings

FIG. 1 is a schematic perspective view of a biochip analyzer according to an embodiment of the present invention;

FIG. 2 is a schematic view showing a configuration of a biochip analyzer in another state according to the embodiment of the present invention;

FIG. 3 is a schematic structural view of a chip loading device according to an embodiment of the present invention, wherein a chip magazine is in an open position;

FIG. 4 is a schematic view showing the structure of a chip loading apparatus according to an embodiment of the present invention, in which a chip magazine is in a closed position (a part of the structure is omitted);

FIG. 5 is a schematic structural view of the die loading member of FIG. 3;

FIG. 6 is a schematic structural view of the chip magazine in FIG. 3;

FIG. 7 is a schematic view of a disassembled structure of the feeding gripper device in the embodiment of the present invention;

FIG. 8 is a schematic view of the structure of FIG. 7 from another perspective (disassembled);

FIG. 9 is a schematic structural diagram of an eight-row clamping object pipe assembly of the feeding gripper device according to an embodiment of the present invention;

FIG. 10 is a schematic view of the structure of FIG. 9 from another perspective;

FIG. 11 is a schematic illustration of the feeding gripper apparatus of FIG. 7 prior to clamping of an eight gang tube assembly;

FIG. 12 is a schematic view of the clamping of an eight gang tube assembly by the feed gripper apparatus of FIG. 7;

FIG. 13 is a schematic diagram of the loading gripper of FIG. 7 before clamping a microfluidic chip;

FIG. 14 is a schematic view of a microfluidic chip clamped by the feeding gripper of FIG. 7;

FIG. 15 is a schematic structural diagram of a micro droplet chip bonding apparatus according to an embodiment of the present invention;

FIG. 16 is a schematic view of a disassembled structure of the base plate and the chip and reaction tube assembly shown in FIG. 15;

FIG. 17 is a schematic view of a disassembled structure of a chip and a reaction tube assembly according to an embodiment of the present invention;

FIG. 18 is a schematic view of the micro droplet chip bonding apparatus according to the embodiment of the present invention in a state where no bonding force is applied to the chip;

FIG. 19 is a schematic view of a micro droplet chip bonding apparatus according to an embodiment of the present invention, in which a bonding force is applied to a chip, and the bonding force is denoted by F;

FIG. 20 is a schematic perspective view of the waste collection bin in an embodiment of the present invention showing the sealing lid in a first position and the pull base in a pulled-out position;

FIG. 21 is a perspective view of the waste collection box in the embodiment of the present invention, showing the sealing lid at the second position and the drawing base at the storing position;

FIG. 22 is a perspective view of the waste collection bin in an embodiment of the present invention, showing the sealing lid in a first position and the drawer base in a storage position;

fig. 23 is a perspective view of the drawer base of fig. 20.

The reference numerals are represented as:

1. a frame; 2. a PCR amplification device; 21. a first housing; 22. a heating platform; 23. a sample placement area to be detected; 3. a chip feeding device; 31. a chip material box; 311. a lifting channel; 32. a chip feeding part; 321. a chip supporting claw; 322. mounting a bracket; 323. a lifting guide rail; 324. a lifting drive motor; 325. a lifting screw rod; 3261. an upper photoelectric switch; 3262. a lower photoelectric switch; 327. an inductive contact blade; 328. reinforcing ribs; 33. a mounting substrate; 331. a first slide rail; 332. a first connecting slider; 333. a first link; 334. a first photoelectric switch; 335. a first trigger piece; 336. a first hydraulic buffer; 337. a limiting block; 34. a cartridge base; 341. a dome sensor; 35. an opening and closing cylinder; 36. a bracket is magnetically attracted; 361. an electromagnet; 362. a second hydraulic buffer; 4. a sample detection device; 5. a transfer device; 51. a first guide rail; 52. a second guide rail; 6. a waste recovery tank; 7. a liquid storage device; 71. a liquid storage housing; 72. a liquid storage box; a1, electric clamping jaws; a11, a first translational claw; a12, a second translational claw; a2, a first clamping block; a21, a first exhaust pipe clamping part; a22, a first chip clamping part; a23, positioning pins; a24, jackscrew; a3, a second clamping block; a31, a second tube bank clamping part; a32, a second chip clamping part; a41, eight-row pipe bodies; a42, arranging seats; a421, arranging a seat groove; a422, arranging the seat lug; a43, a tube cover; a51, a sensor bracket; a52, a photoelectric sensor; a6, a microfluidic chip; a100, a movement mechanism; b1, a base plate; b11, a first supporting plate; b12, a second support plate; b121, a high-position photoelectric sensing component; b122, a low photoelectric sensing component; b13, sliding force application plates; b131, a photoelectric trigger; b14, a through shaft type screw rod motor; b141, a shell; b142, a screw rod connecting piece; b15, mounting a plate; b16, positioning a groove; b17, objective lens through holes; b2, a chip and a reaction tube assembly component; b21, chip holder; b22, chip; b23, a calandria bottom support; b24, oiling needle; b25, a calandria seat; b26, a tube cover; b27, a reaction tube; b28, a chip guide shaft; b31, a fixed seat; b311, mounting grooves; b32, a guide block; b41, a guide rod; b42, a coil spring; b51, guide rails; b52, a sliding block; b6, objective lens; c12, bottom plate; c13, a rear side vertical plate; c131, a first guide rail; c14, a left vertical plate; c141, a photosensor; c15, a right vertical plate; c16, a front side vertical plate; c2, sealing the cover; c22, an ultraviolet lamp panel; c23, a material level detection component; c31, driving a motor; c32, belt wheel; c33, a driven wheel; c34, synchronous belt C34; c35, connecting plates; c36, a driving device mounting plate; c37, a first groove-shaped optical coupler; c4, drawing and pulling the base; c41, a base bottom plate; c42, a base rear plate; c43, a base left plate; c44, a base right plate; c45, a base front plate; c46, a guide rail sliding assembly; c47, a guide plate; c48, a first spring; c5, magnetic attraction devices; c6, a buffer device; c7, a second groove-shaped optical coupler; and C8, moving the barrel.

Detailed Description

Referring to fig. 1 to 23 in combination, according to an embodiment of the present invention, there is provided a biochip analyzer, including a housing 1, the frame 1 is provided with a PCR amplification device 2, a chip feeding device 3 and a sample detection device 4, the PCR amplification device 2 comprises a first housing 21, a heating platform 22, the heating platform 22 having a retracted position within the first housing 21 and an extended position outside the first housing 21, the top of the first housing 21 is configured with a sample placement area 23 to be tested, and further comprises a transfer device 5, the transfer device 5 is capable of transferring the sample on the heating platform 22 in the extended position to the sample placement area 23 to be tested, transferring the sample from the sample placement area 23 to be tested to the sample testing device 4, and can transfer the chips in the chip loading device 3 to the sample testing device 4. In the technical scheme, the transfer device 5, the PCR amplification device 2, the chip feeding device 3 and the sample detection device 4 are adopted to cooperatively act with a plurality of functional devices, so that the automatic circulation of samples and chips in the PCR amplification, sample and biochip feeding, biochip detection and analysis processes is realized, the automation degree and the integration degree are high, the analysis and detection efficiency is higher, and the labor cost is greatly reduced.

Further, preferably, a waste recycling bin 6 is further installed on the rack 1, and the transferring device 5 can also transfer the chips and/or samples in the sample detecting device 4 into the waste recycling bin 6, and with the adoption of the technical scheme, the chips and samples after detection in the sample detecting device 4 are automatically discarded in the waste recycling bin 6, so that the next detection process can be more quickly performed, for example, the samples are obtained from the PCR amplification device 2 again, and the chips are obtained from the chip loading device 3, so that the detection efficiency is further improved.

Preferably, the transfer device 5 includes a first guide rail 51 and a second guide rail 52 erected on the upper area of the frame 1, the first guide rail 51 is provided with two first guide rails 51, the two first guide rails 51 are arranged in parallel and respectively located at two ends of the length of the second guide rail 52, the second guide rail 52 can linearly reciprocate along the length extending direction of the first guide rail 51, and a feeding gripper device is slidably connected to the second guide rail 52.

Referring to fig. 7 to 14 in combination, the feeding gripper device, which may be used with a motion mechanism a100, such as the aforementioned guide rail, to achieve a wide range of position shifts, includes an electric gripper A1, where the electric gripper A1 includes a first translational gripper a11 and a second translational gripper a12 that can move opposite or away from each other, a first gripper block a2 is connected to the first translational gripper a11, a second gripper block A3 is connected to the second translational gripper a12, a first row tube gripper a21 is configured on a side of the first gripper block a2 facing the second gripper block A3, a second row tube gripper a31 is configured on a side of the second gripper block A3 facing the first gripper block a2, the electric gripper A1 is implemented by using an electric gripper on the market, and as long as it can ensure that there are two components that can generate opposite or away from each other functionally, a first gripper block a2 is implemented by a first row tube gripper a22, first chip clamping part A22 is in the top of first calandria clamping part A21, still be constructed second chip clamping part A32 on second centre gripping piece A3, second chip clamping part A32 is in the top of second calandria clamping part A31, first chip clamping part A22 with second chip clamping part A32 both form the centre gripping to micro-fluidic chip A6 jointly. In this technical scheme, through electronic clamping jaw A1 realizes the centre gripping action, and through set up on first clamping block A2 and second clamping block A3 in calandria structure looks adaptation first calandria clamping part A21, second calandria clamping part 22, and first chip clamping part A22, the clamping action to calandria and chip is realized to second chip clamping part A32, simple structure is compact, can carry out automation, it is accurate, snatch reliably, shift and place calandria and chip, satisfy PCR testing process's long time, high speed, fast beat demand.

Further, as a specific implementation mode, the calandria structure is eight calandria subassembly even, also the material loading hand claw device is used for eight calandria subassemblies that link of centre gripping, eight calandria subassemblies include eight calandria body A41 and be used for fixing eight row seat A42 that links calandria body A41, eight mouth of pipe sealing connection that links calandria body A41 has tube cap A43, the first end of the length of row seat A42 be equipped with row seat recess A421 that the lug corresponds, the length second end of row seat A42 be equipped with row seat lug A422 that the recess corresponds, the setting of row seat A42 can prevent the complicated problem that is difficult to realize of structure that direct contact leads to between first calandria clamping part A21 and second calandria clamping part A31 and eight row pipe body A41 from taking place. At this time, corresponding to the structure of the bank seat a42, the first bank pipe clamping portion a21 includes a convex protrusion extending toward the second bank pipe clamping portion a31 side, and the second bank pipe clamping portion a31 includes a groove corresponding to the position of the protrusion. The row seat bump a422 is preferably provided with an information carrier component, such as a two-dimensional code information label, etc., which can store the sample information in the eight-row tube body a41, and specifically, the row seat bump a422 has a plane of 6mm × 7mm, and can store information by laser printing of a two-dimensional code.

A chamfer is arranged at the edge of the lug facing the row seat groove A421; and/or the edge of the groove facing the row seat bump A422 is provided with a chamfer angle, so that the position error of +/-0.1 mm is allowed, and the self-adaptive clamping can be realized within the error range.

Specifically, the first chip clamping portion a22 includes a first chip clamping groove, the second chip clamping portion a32 includes a second chip clamping groove, and edges of the microfluidic chip a6 are inserted into the first chip clamping groove and the second chip clamping groove, so that the microfluidic chip a6 can ascend or descend along with the feeding gripper device. Preferably, a positioning pin a23 is further inserted into the first chip clamping groove, and the positioning pin a23 passes through the first chip clamping groove in the vertical direction of the use orientation of the feeding gripper device to horizontally position the microfluidic chip a 6; and/or a positioning pin A23 is further inserted into the second chip clamping groove, the positioning pin A23 passes through the second chip clamping groove along the vertical direction of the using direction of the feeding paw device to horizontally position the microfluidic chip A6, at this time, the positioning pin A23 limits the horizontal degree of freedom of the microfluidic chip A6, specifically, a semicircular arc-shaped groove is formed on the edge wall body of the microfluidic chip A6, and the semicircular arc-shaped groove is matched with the cylindrical surface of the positioning pin A23 to limit the horizontal degree of freedom, so that the microfluidic chip A6 can be effectively prevented from slipping out of the chip clamping groove in the transferring process. Similarly, the edges of the first chip card slot and the second chip card slot, which are matched with the edge of the microfluidic chip a6, are also provided with oblique angles, so that a position error of +/-0.1 mm is allowed, and self-adaptive clamping can be realized within the error range.

The diameter of the locating pin a23 matches the size of the semicircular arc-shaped slot on the edge wall of the microfluidic chip a6, which in one embodiment is provided as a 4mm diameter cylinder locating pin a 23. It should be further noted that more semicircular arc-shaped grooves can be arranged on the edge wall body of the microfluidic chip a6 and the semicircular arc-shaped grooves for other purposes, such as placement and positioning, but should be kept different in size from the positioning pin a23 to ensure the convenience of clamping and positioning. The positioning pin A23 is fixedly connected to the first clamping block A2 and/or the second clamping block A3 through a jackscrew A24.

Preferably, the feeding gripper device further comprises a photoelectric detection assembly, the photoelectric detection assembly comprises a sensor bracket A51 and a photoelectric sensor A52 connected to the sensor bracket A51, and the photoelectric detection assembly is arranged on the top of the first clamping block A2 and/or the second clamping block A3. The photoelectric detection assembly is used for detecting the position of the eight-row tube assembly or the microfluidic chip A6 and judging whether the position exists or not. The specific effective detection distance of the photoelectric sensor A52 is 2mm-25mm, the electric clamping jaw A1 drives the first translational jaw A11 and the second translational jaw A12 to be opened, the first translational jaw A1 is driven by the movement mechanism A100 to move to the position where the eight-row tube assembly or the micro-fluidic chip A6 is placed, the photoelectric sensor A52 is used for detecting, whether the eight-row tube assembly or the micro-fluidic chip A6 exists at the position can be judged, and when the judgment is 'existence', the grabbing action can be executed to grab and move. When the electric clamping jaw A1 grabs the eight-row tube assembly or the microfluidic chip A6, the photoelectric sensor A52 can judge that the eight-row tube assembly or the microfluidic chip A6 is grabbed, and when the electric clamping jaw A1 puts down the eight-row tube assembly or the microfluidic chip A6 and leaves a certain distance, the photoelectric sensor A52 can judge that the eight-row tube assembly or the microfluidic chip A6 is not grabbed.

In some embodiments, the rack 1 is further provided with a liquid storage device 7, the liquid storage device 7 has a liquid storage housing 71 and a liquid storage box 72 therein, the liquid storage box 72 can be ejected from the liquid storage housing 71, and the liquid storage device 7 is used for providing oil required for forming micro-droplets in the sample detection device 4. The liquid storage box 72 can be popped out from the liquid storage shell 71, so that an operator can supplement oil and overhaul conveniently.

As a specific embodiment of the chip feeding device 3, preferably, the chip feeding device 3 includes a chip magazine 31 and a chip feeding part 32 located at one side of the chip magazine 31, the chip magazine 31 has a stacking cavity for stacking chips, one side of the chip magazine 31 has a lifting channel 311, the lifting channel 311 connects the stacking cavity with the outside of the chip magazine 31, and the chip feeding part 32 includes a chip claw 321, and the chip claw 321 extends into the lifting channel 311. In the technical scheme, the chip in the stacking cavity can be supported from a low position to a high position by the chip supporting claw 321, so that the chip in the stacking cavity can be exposed at the opening of the stacking cavity, the chip exposed at the top of the feeding claw device can be conveniently transferred, the transfer mechanization of the chip is realized, and the labor intensity of an operator is reduced.

Further, the chip feeding component 32 further includes a mounting bracket 322, a lifting guide rail 323 is arranged on the mounting bracket 322, the chip supporting claw 321 is movably connected to the lifting guide rail 323, and the chip supporting claw 321 is further sleeved on a lifting screw rod 325 coaxially connected with a power output shaft of the lifting driving motor 324.

Furthermore, the mounting bracket 322 is further provided with an upper photoelectric switch 3261 and a lower photoelectric switch 3262, wherein the upper photoelectric switch 3261 is disposed at a preset highest ascending position of the chip supporting claw 321, and is used for limiting an highest ascending position of the chip supporting claw 321, it can be understood that, at this time, if the upper photoelectric switch 3261 is triggered to generate a signal, it indicates that the chip in the stacking cavity is lifted to a position to be grasped, and when the chip in the stacking cavity is completely transferred, the upper photoelectric switch 3261 is not triggered, that is, does not send a signal, at this time, an operator should supplement the stacking chip, and the lower photoelectric switch 3262 is disposed at a preset lowest descending position of the chip supporting claw 321, and correspondingly detects whether the chip supporting claw 321 is in place by position resetting. Preferably, the chip supporting claw 321 is fixedly connected with a sensing contact 327, and the sensing contact 327 is used in pair with the upper photoelectric switch 3261 and the lower photoelectric switch 3262, so that the detection accuracy of the upper photoelectric switch 3261 and the lower photoelectric switch 3262 can be improved.

The chip feeding device further comprises a mounting substrate 33, the bottom end of the mounting bracket 322 is fixedly connected with the mounting substrate 33, and further, the mounting substrate 33 is connected with the mounting bracket 322 through a reinforcing rib 328, so that the connection reliability of the mounting bracket 322 and the mounting substrate 33 is improved.

In some embodiments, a magazine seat 34 is disposed on the mounting substrate 33, the chip magazine 31 is mounted in a magazine placing cavity of the magazine seat 34, the material box base 34 is pivotally connected with the mounting base plate 33 through a rotating shaft, the chip feeding device 3 further comprises an opening and closing cylinder 35, the opening and closing cylinder 35 is disposed between the mounting substrate 33 and the magazine base 34 to force the chip magazine 31 to switch between a closed position and an open position, in the technical scheme, the position of the magazine base 34 is switched by the extension and contraction of the opening and closing cylinder 35, so that the chip magazine 31 is switched between the closed position and the open position, when the chip magazine 31 is at the closed position, wherein the chips can be lifted for loading, and when the chip magazine 31 is in the open position, an operator can load the chips into the stacking chamber.

Further, a first slide rail 331 is configured on the mounting substrate 33, a first connection slide block 332 is slidably connected to the first slide rail 331, the first connection slide block 332 is hinged to the magazine base 34 through a first connecting rod 333, a first photoelectric switch 334 is further disposed on the mounting substrate 33, a first trigger piece 335 is disposed on the first connection slide block 332, when the chip magazine 31 is located at the closed position, the first photoelectric switch 334 can be triggered by the first trigger piece 335 to generate a signal, that is, the first connecting rod 333 can convert the rotational opening and closing displacement of the magazine base 34 into the sliding displacement of the first connection slide block 332, and further, whether the magazine base 34 is closed in place is detected through the first photoelectric switch 334.

In some embodiments, the magazine seat 34 is configured with a plurality of magazine placing cavities, the number of the chip magazines 31 matches the number of the magazine placing cavities, and the number of the chip supporting claws 321 matches the number of the chip magazines 31, so as to increase the single chip supply amount of the feeding device, for example, the magazine seat 34 is configured with two magazine placing cavities, and the number of the chip magazines 31 and the number of the chip supporting claws 321 and the number of the chip magazines 31 are both two.

In some embodiments, a first oil buffer 336 is further fixedly connected to the mounting substrate 33, a buffer movable end of the first oil buffer 336 is connected to the first connection slider 332, and when the chip magazine 31 is switched from the closed position to the open position, the first oil buffer 336 applies a force to the first connection slider 332 in a direction opposite to a force application direction of the opening and closing cylinder 35, that is, the first oil buffer 336 can provide a damping force during the opening of the chip magazine 31, so that the chip magazine 31 and the magazine base 34 can descend at a constant speed, and an excessive impact force is avoided.

Further, a limiting block 337 is further disposed on the mounting substrate 33, when the chip magazine 31 is located at the closed position, the limiting block 337 is supported on the bottom wall of the chip magazine 31, so that the magazine seat 34 can be limited, and the upper surface of the limiting block 337 is in surface contact with the bottom wall of the chip magazine 31 to ensure the verticality of the chip magazine 31.

In some embodiments, a magnetic support 36 is further connected to the mounting substrate 33, an electromagnet 361 is connected to the magnetic support 36, and when the chip magazine 31 is in the closed position, the electromagnet 361 can attract the magazine base 34, so that the chip magazine 31 is reliably in the closed position, and it can be understood that when the chip magazine 31 is switched from the closed position to the open position, the electromagnet 361 can be controlled to lose magnetic force. Preferably, the magnetic attraction bracket 36 is further provided with a second oil buffer 362 to prevent the chip magazine 31 from impacting too much when closed, so that the stacked chips therein vibrate too much to cause position deviation.

In some embodiments, the bottom of the magazine placing cavity of the magazine base 34 is provided with a dome sensor 341 for detecting whether the chip magazine 31 is placed properly therein.

Referring to fig. 15 to 19, as a specific implementation manner of the sample testing device, the sample testing device 4 includes a micro droplet chip pressing device, which includes a base plate B1, the base plate B1 is provided with a first support plate B11 and a second support plate B12 which are arranged at an interval, and further includes a sliding force application plate B13 and a linear displacement driving device, two ends of the sliding force application plate B13 are respectively connected with the first support plate B11 and the second support plate B12 in a sliding manner in a one-to-one correspondence manner, a placement space for a chip and reaction tube assembly B2 is formed between the sliding force application plate B13 and the base plate B1, and the linear displacement driving device is configured to drive the sliding force application plate B13 to move towards or away from the base plate B1 in a linear manner. In this technical solution, the linear displacement driving device can drive the sliding force application plate B13 to move linearly up and down, when the chip and reaction tube assembly B2 is placed in the placement space, the downward sliding force application plate B13 will contact the upper surface of the chip B22 in the chip and reaction tube assembly B2 (i.e. the side away from the chip holder B21) and gradually press down the chip B22, so that the tip of the lower surface of the chip B22 (i.e. the side close to the chip holder B21) contacts the tube cover B26 in the tube array component in the chip and reaction tube assembly B2, and further pierce the tube cover B26, so that the reaction liquid in the reaction tube B27 can selectively communicate with the corresponding tube of the chip B22, thereby the micro droplet chip crimping device of the present invention can automatically pierce and crimp the chip and the reaction tube cover, the detection work efficiency is improved.

Specifically, the opposite side surfaces of the first support plate B11 and the second support plate B12 are respectively connected with a guide rail B51, the sliding force application plate B13 is provided with a slider B52 corresponding to the two ends of the first support plate B11 and the second support plate B12, and a concave-convex structure is arranged between the slider B52 and the guide rail B51 to realize the sliding connection.

As a specific embodiment of the chip and reaction tube assembly B2 adapted to the micro droplet chip crimping apparatus of the present invention, the chip and reaction tube assembly B2 comprises, in addition to the chip holder B21, the chip B22, the tube array module, the tube array base B23, the chip guide shaft B28 and the oil injection needle B24, the tube array module comprises the reaction tube B27 and the tube cover B26 covering the nozzle of the reaction tube B27, the reaction tube B27 is seated on the tube array holder B25, so as to achieve the mechanical clamping transfer of the reaction tube B27 (eight tubes are used as a specific example in the present invention) by clamping the tube array holder B25, as shown in fig. 3, the chip holder B21 serves as a common base for the chip B22 and the tube array module, the tube B23 is fixedly connected below the chip holder B21, and the oil injection needle B24 is fixedly connected above the chip holder B21, the chip guide shaft B28 is fixedly connected to the holes above the two ends of the oil injection needle B24, the reaction tube B27 is seated in the holes provided in the tube seat B25, the tube cover B26 is located on the tube mouth of the reaction tube B27, the tube seat B25, the reaction tube B27 and the tube cover B26 are located as a whole, i.e. forming a tube bank assembly, on the chip seat B21, the chip B22 is located above the chip seat B21 and contacts with the tube cover B26 below, and the chip B22 has a tip position corresponding to the tube cover B26, it can be understood that, although they are in contact, when the corresponding pushing-down force (crimping force) is not applied, the tip does not pierce the tube cover B26 corresponding thereto, and further, the chip B22 and the tube seat B25 have a snap structure therebetween, similarly, when the tube seat B22 is placed above the tube seat B25 corresponding to the pushing-down force applied by the weight of itself only when the tube seat B2 is not applied, the snap structure will be pressed to connect the chip B22 and the socket B25 as a whole.

The linear displacement driving device comprises a through shaft type screw rod motor B14, a shell B141 of a through shaft type screw rod motor B14 is fixedly connected to a mounting plate B15, a screw rod connecting piece B142 of the through shaft type screw rod motor B14 is fixedly connected to one side, deviating from the base plate B1, of the sliding force application plate B13, the mounting plate B15 is erected between the first supporting plate B11 and the second supporting plate B12, and the sliding force application plate B13 can realize linear reciprocating motion in the height direction through the adoption of the through shaft type screw rod motor B14, so that the structure is compact.

Furthermore, in order to limit the limit position of the vertical linear motion of the sliding force application plate B13, a photoelectric trigger B131 is disposed on the sliding force application plate B13, a high position photoelectric sensing element B121 and a low position photoelectric sensing element B122 are disposed on the second support plate B12 along the linear motion path of the sliding force application plate B13, and the high position photoelectric sensing element B121, the low position photoelectric sensing element B122, and the photoelectric trigger B131 cooperate to define the position of the sliding force application plate B13.

Preferably, the base plate B1 is provided with two limiting members, and the two limiting members are spaced apart from each other on the side of the base plate B1 facing the sliding force application plate B13, and are used for limiting the chip and reaction tube assembling assembly B2 in the horizontal direction and the vertical direction (at a distance). Specifically, the limiting assembly comprises a fixed seat B31 and a guide block B32, wherein a mounting groove B311 is formed in the fixed seat B31, the guide block B32 is inserted into the mounting groove B311 and partially protrudes out of one side of the fixed seat B31, which is close to the chip and reaction tube assembling assembly B2, and a threaded hole connected with the guide block B32 is formed in a chip seat B21 in the chip and reaction tube assembling assembly B2. The guide block B32 can restrict the X-Y degree of freedom of the chip and reaction tube assembly B2 in the horizontal direction (parallel to the upper surface of the base plate B1), and can adjust the Z degree of freedom of the chip and reaction tube assembly B2 in the height direction (perpendicular to the upper surface of the base plate B1), that is, the height in the Z direction, which facilitates the adjustment of the distance between the chip B22 and the objective lens B6 under the chip B22 and corresponding to the position of the observation window provided on the chip B22, thereby making the field of view clearer.

Furthermore, a positioning groove B16 is further configured on the base plate B1, and the bottom end of the guide block B32 is located in the positioning groove B16, at this time, the positioning groove B16 can prevent the guide block B32 from coming out of the fixing seat B31, so that the guiding reliability of the guide block B32 on the chip and reaction tube assembling component B2 is ensured.

The base plate B1 is further provided with four sets of elastic members, two of which are disposed at the bottom of the first side of the chip holder B21, and the other two are disposed at the bottom of the second side of the chip holder B21, the first side being opposite to the second side, the elastic members being capable of being compressed after the chip and reaction tube assembly B2 is placed in the placing space, thereby achieving a floating equilibrium state of the entire position of the chip and reaction tube assembly B2, at which time, the bottom surface of the chip holder B21 is not in contact with the upper plane of the base plate B1, i.e., there is a gap therebetween, which is 3mm in one embodiment, and when the upper sliding force applying plate B13 applies downward force to the chip and reaction tube assembly B2, the elastic members are further compressed, at this time, the bottom surface of the chip holder B21 contacts the upper plane of the base plate B1, the gap is eliminated, and correspondingly, the tip of the bottom of the chip B22 pierces the tube cover B26 to form a controlled communication between the micro-droplet channel on the chip B22 and the micro-droplets in the reaction tube B27, and the chip B22 and the chip holder B21 form a snap connection to form a structurally reliable whole with the chip and the reaction tube assembly B2. Specifically, the elastic component comprises a guide rod B41 used for connecting the base plate B1 and a spiral spring B42 sleeved on the guide rod B41, the bottom wall of the chip seat B21 is provided with a circular groove matched with the spiral spring B42, the spiral spring B42 is limited in the horizontal direction and the vertical direction, and when the chip and reaction tube assembly B2 is placed in the placing space, the spiral spring B42 is supported on the bottom wall of the chip seat B21. Further, the base plate B1 is formed with an objective lens passing hole B17 for placing an objective lens B6, and the position of the objective lens passing hole B17 is adapted to the observation window of the chip B22 in the chip and reaction tube assembly B2, but it is understood that the objective lens B6 may be structurally fixed to the base plate B1, and may be separately provided independently from the base plate B1. By adopting the technical scheme, after the chip B22 and the tube cover B26 are in compression joint, the through shaft type lead screw motor B14 slightly moves upwards, the elastic force of the spiral spring B42 prompts the chip and the reaction tube assembly component B2 to move upwards, the elastic force of the spiral spring B42 can ensure the sealing performance of the chip and the reaction tube, leakage cannot occur, the position of the objective lens B6 is kept unchanged in the process, in the process of the through shaft type lead screw motor B14 moving upwards, whether the objective lens B6 focuses on micro liquid drops in the chip B22 or not is judged through a related algorithm, and optical detection and analysis are performed on the micro liquid drops after focusing is judged.

The micro droplet chip bonding apparatus of the present invention is further described with reference to specific operation steps as follows:

the initial action of the micro-droplet chip automatic crimping device is as follows: the through shaft type lead screw motor B14 rotates to drive the slider B52, the sliding force application plate B13, the lead screw connecting piece B142 and the photoelectric trigger piece B131 to move upwards along the guide rail B51 until the photoelectric trigger piece B131 triggers the groove type optical coupler above (a specific implementation form example of the high position photoelectric sensing component B121 or the low position photoelectric sensing component B122 is the same as the above), and a lead screw on the through shaft type lead screw motor B14 rises to the highest position and stops.

The eight-row tube (an example of a specific implementation manner of the reaction tube B27, the same below) contains micro-droplets amplified by PCR, a tube cover B26 is tightly fastened above the eight-row tube B27, the eight-row tube is tightly attached to the hole of the tube seat B25, the tube seat B25, the eight-row tube B and the tube cover B26 are integrally placed above the chip seat B21 by a manipulator or a human hand, the lower portion of the eight-row tube is located in the hole of the chip seat B21, and is constrained by structure and gravity, when the position detection mechanism detects that the tube seat B25 is in place, the manipulator or the human hand places the chip B22 above the chip seat B21, the circular holes on both sides of the chip B22 are matched with the chip guide shaft B28, the precise placement of the chip B22 is completed by structure and gravity, the tip penetrating through the tube cover B48 below the chip B22 is in contact with the tube cover B26, and the lower surface of the chip B22 is not in contact with the upper surface of the chip seat B21. Under the combined action of the elasticity of the spiral spring B42 and the structural limit of the fixed seat B31, a 3mm gap is formed between the bottom surface of the chip seat B21 and the upper surface of the base plate B1.

When the position detection mechanism detects that the chip B22 is in place, the through-shaft lead screw motor B14 acts again. The screw rod on the through shaft type screw rod motor B14 drives the sliding block B52, the sliding force application plate B13, the screw rod connecting piece B142 and the photoelectric trigger piece B131 to move downwards along the guide rail B51 from the highest point, the lower surface of the sliding force application plate B13 is in contact with the upper surface of the chip B22, at the moment, the chip B22 is pressed by the sliding force application plate B13, the chip B22 and the pipe arranging assembly compress the spiral spring B42 and integrally move downwards by taking the groove of the fixed seat B31 as a guide, the screw rod continues to move downwards until the photoelectric trigger piece B131 triggers the groove type optical coupler below, at the moment, the lower surface of the chip seat B21 is in contact with the upper surface of the base plate B1, and large pressure transmitted by the through shaft type screw rod motor B14 is arranged between the two surfaces. In the process, the chip B22 and the calandria assembly move downwards under the pressure of the sliding force application plate B13, the tip of the lower portion of the chip B22, which is used for penetrating the tube cover B26, finally penetrates the tube cover B26 and is tightly attached to the tube cover B26, and the buckle of the lower portion of the chip is buckled with the calandria seat B25, so that the chip B22, the calandria seat B25, the eight-connection calandria and the tube cover B26 are integrated. At this time, the lower surface of the die B22 is in contact with the upper surface of the die holder B21, and a large pressure is transmitted between the two surfaces by the through-shaft lead screw motor B14.

After a tube cover B26 is pierced below the chip B22, amplified micro droplets contained in the eight-row tubes enter a channel in the chip B22 under the guidance of a control system and auxiliary oil, an optical path emits excitation light to the droplets in the channel of the chip B22 through an objective lens B6, then emitted light with specific wavelength in the droplets is collected through an objective lens B6, and then analysis is completed through an algorithm. The distance between the objective lens B6 and the chip B22 needs to be kept within a specific precision range to ensure the objective lens to be focused, so that the photographed image of the channel of the chip B22 is clearer to complete the algorithm analysis. Therefore, after the chip B22 pierces the tube cover B26 and the photoelectric trigger B131 triggers the slot type optical coupler, the through-shaft lead screw motor B14 acts in reverse direction to make the lead screw thereon move upward slowly, and the chip B22 moves upward with the chip and reaction tube assembly B2 under the elastic force of the coil spring B42. In the process, the optimal position of the chip in the height direction is found through an algorithm, the channel image on the chip B22 is clearest, and the analysis of the DNA solution is most facilitated, and the spiral spring B42 needs to provide enough elasticity to ensure the tightness of the chip B22 and the oil injection needle B24, so that liquid cannot leak.

After the analysis is finished, the motor acts to drive the sliding block B52, the sliding force application plate B13, the screw rod connecting piece B142 and the photoelectric trigger piece B131 to move upwards along the guide rail B51 until the photoelectric trigger piece B131 triggers the groove-shaped optical coupler above, and the screw rod on the through shaft type screw rod motor B14 rises to the highest position and stops. The tightly fastened chip B22, the tube arranging seat B25, the eight-connected tube and the tube cover B26 are taken out by a manipulator or a human hand and discarded in a waste bin. The tube seat B25, the eight row tubes, the tube cover B26, the chip B22 and the like are replaced, and a new round of puncture and detection analysis is started.

As a specific implementation manner of the scrap receptacle box 6, referring to fig. 20 to 23 in combination, the scrap receptacle box 6 includes a receptacle box body having an upward opening, on which a sealing cover C2 is disposed, and a driving device, wherein the sealing cover C2 can slide linearly relative to the receptacle box body under the action of the driving device, so that the sealing cover C2 has a first position where the opening is completely opened and a second position where the opening is completely covered. In the technical scheme, the sealing cover C2 has a first position enabling the opening to be completely opened and a second position enabling the opening to be completely covered, namely the sealing cover C2 is controlled to be at the first position when waste needs to be thrown in, and the sealing cover C2 is controlled to be at the second position when the waste is thrown in, so that the inside and the outside of the recycling bin can be effectively isolated, adverse effects on biochips in each PCR instrument unit arranged adjacent to the recycling bin are avoided, and cross contamination is avoided. The waste specifically comprises a chip, a reaction tube and the like after detection is finished.

In some embodiments, the driving device includes a driving motor C31, a pulley C32 connected to a power output shaft of the driving motor C31, a driven pulley C33 spaced from the pulley C32, and a synchronous belt C3434 tensioned between the pulley C32 and the driven pulley C33, wherein a connecting plate C35 is fixedly connected to the synchronous belt C3434, and the connecting plate C35 is fixedly connected to the sealing cover C2, so that the synchronous belt C3434 is driven to generate linear displacement by controlling the operation or non-operation and rotation direction of the driving motor C31, and the sealing cover C2 fixedly connected to the synchronous belt C3434 is driven to generate linear sliding by the synchronous belt C3434.

Preferably, the recycling storage box body comprises a bottom plate C12, the bottom plate C12 is arranged opposite to the sealing cover C2, a rear vertical plate C13 is connected to the bottom plate C12, the driving device further comprises a driving device mounting plate C36, the driving motor C31 and the driven wheel C33 are fixedly connected with the rear vertical plate C13 through the driving device mounting plate C36, so that the driving devices can be assembled with the rear vertical plate C13 after being integrally mounted through the driving device mounting plate C36, the assembling process of the recycling storage box can be simplified, and the assembling efficiency can be improved.

In some embodiments, a first groove-shaped optical coupler C37 is arranged adjacent to the driven wheel C33, the connecting plate C35 is provided with a first clamping piece, when the sealing cover C2 is in the second position, the first clamping piece can be positioned in a groove of the first groove-shaped optical coupler C37, and at the moment, the first groove-shaped optical coupler C37 generates corresponding control signal feedback to indicate that the sealing cover C2 is in the second position and outputs a corresponding judgment signal for controlling the driving motor C31.

In order to ensure the smoothness of the linear sliding process of the sealing cover C2, preferably, the rear vertical plate C13 is further provided with a first guide rail C131, the first guide rail C131 extends along the linear sliding direction of the sealing cover C2, and the sealing cover C2 is slidably connected with the first guide rail C131 through a first slider (not shown in the figure).

It can be understood that the recycling container further includes a left vertical plate C14, a right vertical plate C15, and a front vertical plate C16, the left vertical plate C14 and the right vertical plate C15 are respectively located at two sides of the rear vertical plate C13 and are connected to the top surface of the bottom plate C12, preferably, a drawing base C4 is further provided in the recycling container, and has a base accommodating cavity, a moving barrel C8 is located in the base accommodating cavity, the moving barrel C8 can be taken out from the base accommodating cavity, so as to take out the waste stored inside after reaching a preset amount and perform subsequent recycling treatment, the drawing base C4 is connected with the front vertical plate C16, the drawing base C4 has a storage position in the recycling container and a drawing position outside the recycling container, when the drawing base C4 is in the storage position, the left vertical plate C14, the right vertical plate C15, the front vertical plate C16 and the rear vertical plate C13 are arranged together to form a side vertical wall of the recovery and accommodation box body.

In some embodiments, the drawing base C4 includes a base bottom plate C41, a base rear plate C42, a base left plate C43, a base right plate C44 and a base front plate C45, wherein the base left plate C43 and the base right plate C44 are slidably connected to the left vertical plate C14 and the right vertical plate C15 through rail sliding assemblies C46, the front vertical plate C16 is connected to the base front plate C45, and the drawing process of the drawing base C4 can be more stable by providing two rail sliding assemblies C46 on the left and right sides of the drawing base C4. The guide rail sliding assembly C46 includes a second guide rail and a second sliding block slidably connected to the second guide rail, wherein the second guide rail is connected to the left vertical plate C14 and the right vertical plate C15, respectively, and the second sliding block is connected to the base left plate C43 and the base right plate C44, respectively.

As a more excellent real-time mode, be equipped with guide plate C47 in pull base C4, guide plate C47 with base backplate C42 parallel interval sets up, guide plate C47's top has the direction inclined plane, so as to can be right remove bucket C8 to the installation of the seat that the base holds the intracavity forms the direction, just guide plate C47 with be provided with first spring C48 between the base backplate C42, first spring C48 can force guide plate C47 has and keep away from base backplate C42's linear motion trend, thereby can be right remove bucket C8's bucket wall forms the clamp force, effectively guarantees remove bucket C8 is in the positional reliability in pull base C4.

In some embodiments, a magnetic attraction device C5 is disposed on the bottom panel C12, the magnetic attraction device C5 is capable of generating attraction force on the base rear panel C42 when the drawing base C4 is in the storage position, for example, the magnetic force attracting device C5 includes an energized demagnetizing electromagnet, which has an electromagnetic force when it is not energized (power is turned off), thereby forming suction to the chassis rear panel C42, thereby enabling the drawing chassis C4 to be reliably placed in the stored position, this design ensures that the drawer C4 is reliably sucked into the storage position even after the power supply to the device is cut off, it will be understood that, when the drawing seat C4 is switched from the storage position to the drawing position due to the need to take out waste, the energized degaussing electromagnet will be energized, thereby losing electromagnetic attraction to the base back plate C42.

Preferably, a buffer device C6 is further arranged on the bottom plate C12, the buffer device C6 includes a second spring, when the drawing base C4 is located at the accommodating position, the second spring can apply force on the base rear plate C42 to make the drawing base C4 have a movement trend towards the drawing position, at this time, when the magnetic attraction device C5 is controlled to lose the electromagnetic force of response, under the action of the second spring, the drawing base C4 will be ejected out a distance from the inside of the recovery containing box, so that an operator can draw the drawing base C4 conveniently without constructing a hand-picking position on the front side vertical plate C16.

Further, still be equipped with second cell type opto-coupler C7 on the bottom plate C12, the second card has on the base backplate C42, works as pull base C4 is in during the position of accomodating, the second card can be in the inslot that second cell type opto-coupler C7 has, through setting up of second cell type opto-coupler C7 can be right pull base C4's position detects, and pull base C4 is in during the position of accomodating, the corresponding signal of feedback extremely magnetic force actuation device C5 makes it produce corresponding actuation, guarantees pull base C4 reliably is in the position of accomodating.

In some embodiments, the left vertical plate 14 and/or the right vertical plate 15 are provided with a photoelectric sensor 141, which is used for detecting whether the drawing base 4 has the movable barrel 8 and detecting whether the movable barrel 8 has a garbage bag therein, and sending a corresponding prompt message to prompt the use state of the garbage collection box, such as whether the movable barrel 8 and the garbage bag are in a predetermined position.

In some embodiments, an ultraviolet lamp panel C22 is disposed on the sealing cover C2, and an ultraviolet emitting surface of the ultraviolet lamp panel C22 faces the opening to sterilize waste materials in the movable barrel C8 by ultraviolet rays, thereby further preventing cross contamination.

Further, a material level detection component C23 is arranged on the sealing cover C2 and is used for detecting the amount of the waste material stored in the recycling accommodating box, and the material level detection component C23 may be a diffuse reflection photoelectric sensor, for example.

In some embodiments, a sealing strip (not shown) is provided between the sealing cover C2 and the opening to further enhance the sealing action of the sealing cover C2.

It is readily understood by a person skilled in the art that the advantageous ways described above can be freely combined, superimposed without conflict.

The present invention is not limited to the above preferred embodiments, and any modifications, equivalent substitutions and improvements made within the spirit and principle of the present invention should be included in the protection scope of the present invention. The above is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, several improvements and modifications can be made without departing from the technical principle of the present invention, and these improvements and modifications should also be regarded as the protection scope of the present invention.

Claims (10)

1. A biochip analyzer is characterized by comprising a rack (1), wherein a PCR amplification device (2), a chip feeding device (3) and a sample detection device (4) are arranged on the rack (1), the PCR amplification device (2) comprises a first shell (21) and a heating platform (22), the heating platform (22) has a retracted position in the first shell (21) and an extended position outside the first shell (21), a sample placement area (23) to be detected is formed at the top of the first shell (21), the biochip analyzer further comprises a transfer device (5), the transfer device (5) can transfer a sample on the heating platform (22) in the extended position to the sample placement area (23) to be detected and transfer the sample from the sample placement area (23) to be detected into the sample detection device (4), and can transfer the chips in the chip loading device (3) to the sample detection device (4).

2. The biochip analyzer according to claim 1, wherein the rack (1) further comprises a waste collection box (6), and the transfer device (5) is further capable of transferring the chips and/or samples in the sample testing device (4) into the waste collection box (6).

3. The biochip analyzer according to claim 1 or 2, wherein the transfer device (5) comprises a first guide rail (51) and a second guide rail (52) erected above the rack (1), the first guide rail (51) is provided with two, the two first guide rails (51) are arranged in parallel and respectively arranged at two ends of the length of the second guide rail (52), the second guide rail (52) can linearly reciprocate along the direction of the length extension of the first guide rail (51), and a feeding gripper device is slidably connected onto the second guide rail (52).

4. The biochip analyzer according to claim 3, wherein the loading gripper device comprises a motorized gripper jaw (A1), the motorized gripper jaw (A1) comprises a first translational jaw (A11) and a second translational jaw (A12) which can move oppositely or oppositely, a first clamping block (A2) is connected to the first translational jaw (A11), a second clamping block (A3) is connected to the second translational jaw (A12), a first calandria clamping part (A21) is formed on one side of the first clamping block (A2) facing the second clamping block (A3), and a second calandria clamping part (A31) is formed on one side of the second clamping block (A3) facing the first clamping block (A2); still be constructed first chip clamping part (A22) on first grip block (A2), first chip clamping part (A22) is in the top of first calandria clamping part (A21), still be constructed second chip clamping part (A32) on second grip block (A3), second chip clamping part (A32) is in the top of second calandria clamping part (A31).

5. The biochip analyzer according to claim 4, wherein the first tube array holder (A21) comprises a bump extending convex towards one side of the second tube array holder (A31), and the second tube array holder (A31) comprises a groove corresponding to the position of the bump.

6. The biochip analyzer according to claim 5, wherein the holder is used for holding an eight-row tube assembly, the eight-row tube assembly comprises an eight-row tube body (A41) and a row seat (A42) for fixing the eight-row tube body (A41), a tube cover (A43) is hermetically connected to a tube orifice of the eight-row tube body (A41), a row seat groove (A421) corresponding to the bump is formed at a first length end of the row seat (A42), and a row seat bump (A422) corresponding to the groove is formed at a second length end of the row seat (A42).

7. The biochip analyzer according to claim 4, wherein the first chip holder (A22) comprises a first chip card slot and the second chip holder (A32) comprises a second chip card slot.

8. The biochip analyzer according to claim 7, wherein the first chip card slot is further inserted with a positioning pin (A23), the positioning pin (A23) passes through the first chip card slot along a vertical direction of the use orientation of the loading gripper device to horizontally position the microfluidic chip (A6); and/or a positioning pin (A23) is also inserted into the second chip clamping groove, and the positioning pin (A23) passes through the second chip clamping groove along the vertical direction of the using direction of the feeding paw device so as to horizontally position the microfluidic chip (A6).

9. The biochip analyzer according to claim 1, wherein the rack (1) further comprises a liquid storage device (7), the liquid storage device (7) comprises a liquid storage housing (71) and a liquid storage box (72) therein, and the liquid storage box (72) can be ejected from the liquid storage housing (71).

10. The biochip analyzer according to claim 1, wherein the chip feeding device (3) comprises a chip magazine (31) and a chip feeding part (32) at one side of the chip magazine (31), the chip magazine (31) has a stacking cavity for stacking chips, one side of the chip magazine (31) has a lifting channel (311), the lifting channel (311) connects the stacking cavity with the outside of the chip magazine (31), the chip feeding part (32) comprises a chip holding claw (321), and the chip holding claw (321) extends into the lifting channel (311).

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