CN215828778U - Nucleic acid detector and chip mounting assembly thereof - Google Patents

Abstract

The utility model discloses a nucleic acid detector and a chip mounting assembly thereof. The chip mounting component comprises a box body, a chip groove for containing the micro-fluidic chip is formed in the box body, the chip groove is provided with a notch facing upwards, the lower end part of the box body is provided with a notch allowing emitted light to enter the chip groove and allowing exciting light to be emitted out of the chip groove, the box body is provided with a right side wall, a notch extending downwards from the upper edge of the right side wall is arranged on the right side wall, and the end part of a piston on the micro-fluidic chip is inserted into the notch and extends to the outside of the box body. The utility model is beneficial to making the structure of the nucleic acid detector compact and the volume small.

Description

Nucleic acid detector and chip mounting assembly thereof

Technical Field

The utility model belongs to the technical field of nucleic acid detection, and relates to a nucleic acid detector and a chip mounting assembly thereof.

Background

The nucleic acid amplification detection technology is a process of providing in vitro amplification conditions for nucleic acid fragments, enabling the nucleic acid fragments to be amplified in an exponential manner in a large quantity, adding a fluorescent dye or a fluorescent marker in the nucleic acid amplification process, detecting the intensity of a fluorescent signal by adopting an optical device, and obtaining a nucleic acid amplification result by analyzing the fluorescent signal. When the nucleic acid amplification reaction is carried out, the reaction system needs to be heated. In addition, the nucleic acid detecting instrument can integrate nucleic acid amplification and detection, and can heat, illuminate, detect and the like a reaction cavity (usually, an amplification reaction cavity) of a detection chip (usually, a microfluidic chip) after the detection chip is placed in the nucleic acid detecting instrument. However, most of the existing nucleic acid detectors aim at horizontal microfluidic chips, and are complex in structure, large in size, heavy in weight, high in cost and complex in operation, and occupy more space.

SUMMERY OF THE UTILITY MODEL

In view of the above technical problems, the present invention provides a nucleic acid detecting instrument and a chip mounting assembly thereof, which are advantageous for making the nucleic acid detecting instrument compact and small in size.

In order to achieve the purpose, the utility model adopts the following technical scheme:

the utility model provides a chip mounting assembly of nucleic acid detecting instrument, includes the box body, be formed with the chip groove that is used for holding micro-fluidic chip in the box body, the chip groove has the notch that faces upwards, the lower tip of box body has can allow the transmission light to get into the chip groove and allow the exciting light to jet out the fretwork portion in chip groove, the box body has the right side wall, be equipped with the breach from its upper edge downwardly extending on the right side wall, for the tip of the last piston of micro-fluidic chip inserts and makes it extend to outside the box body.

Preferably, the notch comprises a first notch and a second notch, the first notch is located at the front side or the rear side of the second notch, and the downward extending depth of the first notch is smaller than that of the second notch.

Preferably, the lower end of the box body is hollowed out, and a supporting bump extending inwards is arranged in the middle of the inner wall of the box body.

More preferably, the box body has a front side wall and a back side wall which are opposite to each other, and a left side wall and a right side wall which are opposite to each other, and the front side wall, the left side wall, the back side wall and the right side wall enclose the chip slot.

More preferably, the bottom ends of the left and right side walls are connected to a base, and the bottom of the rear side wall is located at a distance above the base.

More preferably, a through window is arranged on the rear side wall.

More preferably, the front side wall and the rear side wall are respectively provided with an operation slot for facilitating inserting and pulling out a chip, and the operation slot extends downwards from the upper edge of the front side wall or the rear side wall.

The utility model also adopts the following technical scheme:

a nucleic acid detecting instrument comprising the chip mounting assembly as described above.

Preferably, the nucleic acid detecting apparatus further comprises a heating device, an optical detecting device, and a robot arm assembly,

the heating device is arranged on the front side or the rear side of the chip mounting assembly and used for heating the microfluidic chip in the chip groove;

the optical detection device is arranged below the chip mounting assembly and is provided with a transmitting light emergent part and an excitation light receiving part which are used for facing the lower end part of the microfluidic chip;

the mechanical arm assembly is arranged on the right side of the chip mounting assembly and comprises a mechanical arm capable of moving in the left-right direction, the mechanical arm is provided with a fork, and the fork is provided with a joint groove for clamping the end part of the piston of the microfluidic chip.

Compared with the prior art, the utility model has the following advantages by adopting the scheme:

the chip mounting assembly can insert a vertical micro-fluidic chip into the chip mounting assembly, an optical detection device can be arranged below the chip mounting assembly, a mechanical arm assembly can be arranged on the right side of the chip mounting assembly to drive a piston in the chip to move, and a heating device can be arranged on the front side or the rear side of the chip mounting assembly, so that the structure of a nucleic acid detector is compact and the size of the nucleic acid detector is small.

Drawings

In order to more clearly illustrate the technical solution of the present invention, the drawings needed to be used in the description of the embodiments will be briefly introduced below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and it is obvious for those skilled in the art to obtain other drawings based on the drawings without creative efforts.

FIG. 1 is a schematic view showing a structure of a nucleic acid detecting apparatus according to an embodiment of the present invention after insertion into a microfluidic chip, in which a cover is not shown;

FIG. 2 is a schematic view of the nucleic acid detecting apparatus shown in FIG. 1, viewed from another perspective;

FIG. 3 is a schematic view of the nucleic acid detecting apparatus shown in FIG. 1, in a state where the microfluidic chip is not inserted;

FIG. 4 is a schematic structural diagram of a microfluidic chip;

FIG. 5 is a schematic view of a chip mounting assembly from a perspective view;

FIG. 6 is a schematic view of a chip mounting assembly from another perspective;

FIG. 7 is a right side view of the chip mounting assembly;

FIG. 8 is a schematic view of the heating device from a perspective;

FIG. 9 is a schematic view of the heating device from another perspective;

FIG. 10 is a schematic structural view of a heat-conducting block;

FIG. 11 is a schematic structural diagram of an optical inspection apparatus;

FIG. 12 is a cross-sectional view of an optical detection device;

FIG. 13 is a schematic diagram of a first robot;

fig. 14 is a schematic structural view of the second robot arm.

Wherein,

1. a base; 2. a chip mounting assembly; 3. a heating device; 4. a mechanical arm assembly; 5. an optical detection device;

21. a box body; 211. a front side wall; 212. a rear sidewall; 213. a left side wall; 214. a right side wall; 22. a chip slot; 23. a window is penetrated; 24. an operation slot; 24. supporting the projection; 25. a notch;

31. a mounting seat; 311. positioning the projection; 32. a heat conducting block; 321. a body; 322. heating the surface; 323. a connecting pin; 33. heating the film; 34. an elastic member; 35. a nut;

41. mounting; 411. a slide rail; 42a, a first mechanical arm; 42b, a second mechanical arm; 421. a fork-shaped piece; 422. an engaging groove; 423. a chute; 43. a screw rod; 44. a motor;

51. a support; 51a, a first bracket; 51b, a second bracket; 51c, end caps; 52. a light source; 53. a first light guide; 531. a light emission part; 54. a second light guide; 541. exciting a light receiving section; 55. a lens; 56. an optical filter; 57. a fluorescent sensor;

100. a microfluidic chip; 101. a piston; 101a, neck.

Detailed Description

Preferred embodiments of the present invention will be described in detail below with reference to the accompanying drawings so that the advantages and features of the utility model may be more readily understood by those skilled in the art. It should be noted that the description of the embodiments is provided to help understanding of the present invention, but the present invention is not limited thereto.

According to an embodiment of the present invention, there is provided a chip mounting assembly for a nucleic acid detecting instrument having such a chip mounting assembly, which is capable of automatically performing nucleic acid extraction, amplification and detection. Fig. 1 to 3 show the nucleic acid detecting apparatus, and fig. 4 shows a microfluidic chip 100 suitable for the nucleic acid detecting apparatus, and the microfluidic chip 100 is a vertical microfluidic chip, that is, a plate whose height is at least greater than its width and which is vertically placed as a whole. The sample inlet is located at the upper part of the microfluidic chip 100, and the liquid circulates in the microfluidic chip 100 from top to bottom. The microfluidic chip 100 further includes two pistons 101 horizontally movable in the left-right direction, and the right end of the piston 101 is located outside the microfluidic chip 100 and has a neck portion 101a with a smaller outer diameter, through which the neck portion 101a is engaged with the robot arm assembly 4. The movement of the piston 101 may cause reagents within the microfluidic chip 100 to mix or cause liquid to circulate between the chambers. The lower portion of the microfluidic chip 100 is provided with a plurality of reaction chambers in parallel, specifically, in this embodiment, the number of the reaction chambers is four and the reaction chambers are arranged in parallel along the front-back direction, so as to provide four detection channels.

Referring to FIGS. 1 to 3, the nucleic acid detecting apparatus includes a base 1, the above-described chip mounting assembly 2, a heating device 3, a robot arm assembly 4, and an optical detection device 5. Wherein, the chip mounting component 2 is arranged on the left side part of the base 1, the heating device 3 is arranged on the rear side of the chip mounting component 2, the optical detection device 5 is arranged below the chip mounting component 2, and the mechanical arm component 4 is arranged on the right side of the chip mounting component 2. The nucleic acid detecting instrument further comprises a cover body (not shown in the figure) covering the base 1, the chip mounting component 2, the heating device 3, the optical detecting device 5 and the mechanical arm component 4 are positioned in the cover body, and a slot for inserting the microfluidic chip 100 into the chip mounting component 2 is formed in the cover body.

The chip mounting assembly 2 is used to mount and fix the microfluidic chip 100. As shown in fig. 5 to 7, the chip mounting assembly 2 includes a case 21, a chip slot 22 is formed in the case 21 for accommodating the microfluidic chip 100, the chip slot 22 has an upward facing notch, and the socket on the cover is located right above the chip slot 22 and aligned with the notch.

The box body 21 has opposite front and back side walls 211 and 212, and opposite left and right side walls 213 and 214, the front side wall 211, the left side wall 213, the back side wall 212, and the right side wall 214 enclose the chip slot 22, the bottom ends of the left and right side walls 213 and 214 are connected to the base 1, and the bottoms of the front and back side walls 211 and 212 are located at a distance above the base 1, so that the optical detection device 5 can be located below the chip slot 22 through the bottom of the back side wall 212. The right side wall 214 is provided with a notch 25 extending downward from the upper edge thereof for inserting the right end of the piston 101 of the microfluidic chip 100 and extending the right end out of the box body 21. Specifically, the notch 25 includes a first notch and a second notch, the first notch is located at the front side of the second notch, and the depth of the downward extension of the first notch is smaller than the depth of the downward extension of the second notch. In the process of inserting the microfluidic chip 100, the right end of the piston 101 at the upper part of the chip is inserted into the first notch, and the right end of the piston 101 at the lower part of the chip is inserted into the second notch. The rear side wall 212 is provided with a through window 23 allowing the heating device 3 to pass through and be in contact with the reaction chamber of the microfluidic chip 100. The front side wall 211 and the rear side wall 212 are respectively provided with an operation slot 24 for facilitating the insertion and extraction of the chip, and the operation slot 24 extends downwards from the upper edge of the front side wall 211 or the rear side wall 212.

The lower end of the box body 21 is hollowed out, the middle part of the inner wall of the box body 21 is provided with a supporting bump 24 extending inwards, and the supporting bump 24 is flush with the lower edges of the front side wall 211 and the rear side wall 212. When the microfluidic chip 100 is inserted, the lower surface thereof is located above the supporting bumps 24, and the microfluidic chip 100 is supported and positioned by the supporting bumps 24.

The heating device 2 is used for heating the reaction chamber of the microfluidic chip 100. Referring to fig. 8 to 10, the heating device 3 includes a mounting seat 31, a heat conduction block 32, and a heater. The bottom of the mounting seat 31 is connected to the base 1, for example, fixed to the base 1 by screws. The heat-conducting block 32 is disposed on the mounting seat 31, and the heat-conducting block 32 has a heating surface 322 disposed corresponding to the reaction chamber of the microfluidic chip 100. Specifically, the heating surface 322 may be in contact with or disposed near the reaction region of the detection chip 100 so as to transfer heat to the reaction region of the detection chip 100, and in this embodiment, the heating surface 322 is in contact with the reaction region of the detection chip 100. The heater is provided on the heat conductive block 32, and heats the heat conductive block 32.

The heat conduction block 32 is movably provided on the mount 31. The heating device 3 further comprises an elastic member 34, wherein the elastic member 34 is disposed between the heat conducting block 32 and the mounting seat 31, and two ends of the elastic member 34 respectively abut against the heat conducting block 32 and the mounting seat 31. That is, the distance between the heat-conducting block 32 and the mounting seat 31 can be adjusted appropriately, and even in the case where the assembly error is large, the heating surface 322 can be ensured to be always attached to the reaction chamber of the microfluidic chip 100. The heat conducting block 32 comprises a body 321 and a connecting pin 323 connected to the body 321, a connecting hole matched with the connecting pin 323 is arranged on the mounting seat 31, and the connecting pin 323 can penetrate through the connecting hole in a sliding manner along the length direction; the elastic member 34 is a compression spring sleeved on the connection pin 323. The connecting pin 323 is provided with a screw thread (not shown), the connecting pin 323 is inserted into a nut 35 after passing through the connecting hole, and the nut 35 is screwed with the connecting pin 323. Specifically, the connecting pin 323 extends backward from the rear side of the body 321 to pass through the connecting hole in the mount 31, and locks the nut 35; by rotating the nut 35, the elastic deformation amount of the elastic member 34 can be adjusted, thereby achieving the adjustment of the distance between the heat conduction block 32 and the mounting seat 31. The body 321 has a protrusion extending forward on the left side, and the heating surface 322 is the left side of the protrusion, and is integrally strip-shaped and extends in the left-right direction. The heating surface 322 is further a vertically extending vertical plane. The heat-conducting block 32 is an aluminum block integrally formed of aluminum or an aluminum alloy. The protrusion is inserted into a through window 23 on the rear side of the cartridge body 21 of the chip mounting assembly 22, and can be attached to the reaction chamber of the microfluidic chip 100 therein to heat the reaction chamber.

The heater is specifically a heating film 33, which is attached to the lower side of the body 321 of the heat conducting block 32. The heating film 33 is provided with a power supply connection terminal for connecting a power supply. After the power is turned on, the heating film 33 generates heat and transfers the heat to the heat conduction block 32, and the reaction chamber of the microfluidic chip 100 is uniformly heated by the heat conduction block 32. Further, the heating film 33 is attached to the body 321 of the heat conductive block 32.

The upper part of the mounting seat 31 is formed with a positioning protrusion 311, and the heat conduction block 32 abuts on the positioning protrusion 311. Specifically, the positioning protrusion 311 extends leftward, the right side portion of the heat conduction block 32 abuts against the lower side surface of the positioning protrusion 311, and positioning is performed by the positioning protrusion 311, so that assembly can be facilitated.

The mechanical arm assembly 4 is used for driving the piston to move along the left-right direction. Referring to fig. 1 to 4, 13 and 14, the robot arm assembly 4 includes a mounting frame 41, a robot arm slidably disposed on the mounting frame 41, and a driving device for driving the robot arm to move. The mounting bracket 41 is fixedly arranged on the base 1, for example, by screw fastening. The robot arm has a fork 421, and the fork 421 has an engagement groove 422 for an end of the piston 101 of the microfluidic chip 100 (specifically, the neck portion 101a of the piston 101) to be caught, and the engagement groove 422 has a notch facing upward. In this embodiment, as shown in fig. 13 and 14, the robot arm assembly 4 includes a plurality of robot arms, including at least a first robot arm 42a and a second robot arm 42b, the first robot arm 42a is higher than the second robot arm 42b, the first robot arm 42a and the second robot arm 42b respectively have forks 421, and each fork 421 has an engaging groove 422. The fork 421 of the first mechanical arm 42a and the fork 421 of the second mechanical arm 42b are located on the same side, specifically, the right side, of the microfluidic chip 100. In a plan view, the fork 421 of the first robot arm 42a is offset from the fork 421 of the second robot arm 42b by a distance, that is, the first robot arm 42a and the second robot arm 42b are spaced back and forth to be able to simultaneously couple with the two pistons 101. Specifically, the second robot arm 42b is located at the lower rear side of the first robot arm 42a, i.e., the second robot arm 42b is located at a distance from the rear side of the first robot arm 42a so as to match the positions of the two pistons 101 in fig. 4.

The mount 41 has a slide rail 411 extending in the horizontal direction, and the robot arm is provided on the slide rail 411 so as to be movable in the horizontal direction. Wherein, the slide rail 411 has two and sets up from top to bottom. Referring to fig. 13, the upper slide rail 411 corresponds to the first robot arm 42a, a slide slot 423 is disposed on the first robot arm 42a and is engaged with the slide rail 411, and the slide rail 411 is inserted into the slide slot 423 of the first robot arm 42 a. Referring to fig. 14, the slide rail 411 on the lower side corresponds to the second mechanical arm 42b, a slide slot 423 matching with the slide rail 411 is disposed on the second mechanical arm 42b, and the slide rail 411 is inserted into the slide slot 423 of the second mechanical arm 42 b.

The number of the driving devices is two, and corresponds to the first robot arm 42a and the second robot arm 42b, respectively. Each driving device includes a motor 44 and a lead screw 43 driven by the motor 44 to reciprocate in the left-right direction, and a robot arm is connected to an end of the lead screw 43. The motor 44 is fixedly disposed on the mounting bracket 41. When the motor 44 operates, the screw 43 moves left or right along the left-right direction, and the mechanical arm moves left or right along the slide rail 411, so as to drive the piston 101 of the microfluidic chip 100 to move left or right, and further provide positive pressure or negative pressure for the liquid in the microfluidic chip 100, thereby providing power for liquid circulation.

The robot arm assembly 4 has an initial position in which the microfluidic chip 100 is inserted downward into the chip mounting assembly 2, and during the insertion, the neck portion 101a of the front upper side piston 101 is inserted into the engagement groove 422 of the fork 421 of the first robot arm 42a, and the neck portion 101a of the rear lower side piston 101 is inserted into the engagement groove 422 of the fork 421 of the second robot arm 42b while being connected to both pistons 101; after the connection, the mechanical arm moves leftwards or rightwards correspondingly with the operation of the motor 44, so that the piston 101 is pushed in or pulled out, the liquid flow in the microfluidic chip 100 is driven, power is provided for the liquid flow in the microfluidic chip 100 or the reagent is promoted to be uniformly mixed, and the micro-fluidic chip is simple in structure and small in size.

The optical detection device 5 is used for irradiating the reaction chamber at the lower part of the microfluidic chip 100 and receiving fluorescence excited by an amplification product in the reaction chamber, so as to detect according to fluorescence color, intensity and the like. As shown in fig. 11 and 12, the optical detection device 5 includes a light emitting part 531 and a light receiving part 541 facing the lower end of the microfluidic chip 100, and the lower end of the cartridge 21 includes a hollow part capable of allowing the emitted light to enter the chip slot 22 and allowing the excitation light to exit the chip slot 22, specifically, the lower end of the cartridge 21 is open and the emitted light can enter the cartridge 21 and the excited fluorescence can exit the cartridge 21.

The optical detection device 5 comprises a support 51, an emission light path and an excitation light path arranged on the support 51. The emission light path includes a light source 52 and a first light guide 53 sequentially arranged along the first optical axis, the first light guide 53 has a first end portion adjacent to the light source 52 and a second end portion, and the emission light exit portion 531 is formed on the second end portion of the first light guide 53. The excitation light path includes a lens 55 and a fluorescence sensor 57 arranged in this order along the second optical axis, and a second light guide 54 having a first end portion and a second end portion, and the above-mentioned excitation light receiving portion 541 is formed on the first end portion of the second light guide 54, and the second end portion of the second light guide 54 is adjacent to the lens 55. An included angle which is larger than 0 and smaller than 180 degrees is formed between the first optical axis and the second optical axis. Specifically, in this embodiment, the included angle is smaller than 90 degrees, the first optical axis intersects the horizontal plane in an inclined manner, and the second optical axis intersects the horizontal plane in a perpendicular manner. The emission light emitting portion 531 is formed on the upper end surface of the first light guide 53, and the excitation light receiving portion 541 is formed on the upper end surface of the second light guide 54.

Each detection channel corresponds to one emission light path and one excitation light path. Therefore, the light source 52 detection device includes a plurality of emission light paths arranged in parallel in the left-right direction and a plurality of excitation light paths arranged in parallel in the left-right direction. The light sources 52 are LED lamps, and the light sources 52 of the plurality of emission light paths form an LED light bar extending in the left-right direction.

The first light guide 53 includes a light guide pillar, the holder 51 is provided with a mounting hole, the light guide pillar is inserted into the mounting hole, and the upper end surface of the light guide pillar is located below the chip groove 22 to form a light emitting portion 531. The light guide column is a solid organic glass column. The whole light guide column is obliquely arranged in a cylindrical shape, and the central line of the light guide column coincides with the first optical axis. The upper end of the light guide bar is partially cut to form a planar emission light exit 531, and faces the chip grooves 22 on the upper and rear sides thereof.

The second light guide 54 is an optical fiber, the upper end surface of which is located right below the chip groove 22 to form an excitation light receiving portion 541, and which is provided in the holder 51 to guide the fluorescence above the lens 55.

The holder 51 includes a first holder 51a and a second holder 51b, a lower end portion of the first holder 51a is coupled to the base 1, an emission light path and a second light guide 54 are disposed at an upper portion of the first holder 51a, a lens 55 and a fluorescence sensor 57 are disposed in the second holder 51b, and the second holder 51b is detachably coupled to the first holder 51a and positioned below the upper portion of the first holder 51a for easy maintenance. The lens 55 is movably disposed in the second holder 51b along the second optical axis for focusing. Specifically, the lens 55 is screwed into the second holder 51b, and when focusing is performed, the second holder 51b is removed, the lens 55 therein is rotated, and the height of the lens 55 is adjusted, thereby performing focusing.

The excitation light path also includes a filter 56 between the lens 55 and the fluorescence sensor 57 to filter out stray light outside of a particular wavelength range.

The optical detection device further comprises an end cap 51c, and the end cap 51c is arranged below the fluorescent sensor 57 and is hermetically connected with the second bracket 51b to prevent dust and water.

The working process of the nucleic acid detector is as follows:

inserting the microfluidic chip into the chip mounting assembly, and operating a motor to drive a mechanical arm to move so as to drive a piston to move, so that a sample and a reagent of the microfluidic chip are mixed or the reagent flows into a target chamber until the sample and the reagent enter a reaction cavity for amplification reaction; electrifying the heating device to heat the reaction cavity; after the reaction is finished, the heating is stopped, the optical detection device is electrified, the reaction cavity is irradiated and the excited fluorescence is received, and qualitative and quantitative detection is carried out according to the color and the intensity of the fluorescence.

The nucleic acid detection device has the advantages of compact structure, small volume, simple operation and convenient use, and can automatically realize nucleic acid extraction, amplification and detection.

As used in this specification and the appended claims, the terms "comprises" and "comprising" are intended to only encompass the explicitly identified steps and elements, which do not constitute an exclusive list, and that a method or apparatus may include other steps or elements. As used herein, the term "and/or" includes any combination of one or more of the associated listed items.

It should be noted that, unless otherwise specified, when a feature is referred to as being "fixed" or "connected" to another feature, it may be directly fixed or connected to the other feature or indirectly fixed or connected to the other feature. Further, the description of the upper, lower, left, right, etc. used in the present invention is only with respect to the positional relationship of the respective components of the present invention with respect to each other in the drawings.

It will be further understood that the terms "first," "second," and the like are used to describe various information and that such information should not be limited by these terms. These terms are only used to distinguish one type of information from another and do not denote a particular order or importance. Indeed, the terms "first," "second," and the like are fully interchangeable. For example, first information may also be referred to as second information, and similarly, second information may also be referred to as first information, without departing from the scope of the present disclosure.

The above embodiments are merely illustrative of the technical ideas and features of the present invention, and are preferred embodiments, which are intended to enable those skilled in the art to understand the contents of the present invention and implement the present invention, and not to limit the scope of the present invention. All equivalent changes or modifications made according to the principles of the present invention should be covered within the protection scope of the present invention.

Claims (9)

1. A chip mounting assembly for a nucleic acid detecting instrument, characterized in that: the micro-fluidic chip packaging box comprises a box body, be formed with the chip groove that is used for holding micro-fluidic chip in the box body, the chip groove has the notch that faces upwards, the lower tip of box body has can allow the transmission light to get into the chip groove and allow the exciting light to jet out the fretwork portion in chip groove, the box body has the right side wall, be equipped with the breach from its upper edge downwardly extending on the right side wall, for the supply the tip of the last piston of micro-fluidic chip inserts and makes it extend to outside the box body.

2. The chip-mounted component for a nucleic acid detecting instrument according to claim 1, wherein: the notch comprises a first notch and a second notch, the first notch is positioned on the front side or the rear side of the second notch, and the depth of the downward extension of the first notch is smaller than that of the downward extension of the second notch.

3. The chip-mounted component for a nucleic acid detecting instrument according to claim 1, wherein: the lower tip fretwork of box body sets up, just the middle part of box body inner wall has the support lug of inside extension.

4. The chip-mounted component for a nucleic acid detecting instrument according to claim 3, wherein: the box body is provided with a front side wall, a rear side wall, a left side wall and a right side wall which are opposite to each other, and the front side wall, the left side wall, the rear side wall and the right side wall are enclosed to form the chip groove.

5. The chip-mounted component for a nucleic acid detecting instrument according to claim 4, wherein: the bottom end parts of the left side wall and the right side wall are connected to a base, and the bottom part of the rear side wall is located at a distance above the base.

6. The chip-mounted component for a nucleic acid detecting instrument according to claim 4, wherein: and a through window is arranged on the rear side wall.

7. The chip-mounted component for a nucleic acid detecting instrument according to claim 4, wherein: preceding lateral wall with the back lateral wall is equipped with the operation groove of being convenient for plug chip respectively, the operation groove is from preceding lateral wall or the top edge downwardly extending of back lateral wall.

8. A nucleic acid detecting instrument characterized by: a chip mounting assembly comprising the nucleic acid detecting instrument according to any one of claims 1 to 7.

9. The nucleic acid detecting instrument according to claim 8, wherein: the nucleic acid detector also comprises a heating device, an optical detection device and a mechanical arm assembly,

the heating device is arranged on the front side or the rear side of the chip mounting assembly and used for heating the microfluidic chip in the chip groove;

the optical detection device is arranged below the chip mounting assembly and is provided with a transmitting light emergent part and an excitation light receiving part which are used for facing the lower end part of the microfluidic chip;

the mechanical arm assembly is arranged on the right side of the chip mounting assembly and comprises a mechanical arm capable of moving in the left-right direction, the mechanical arm is provided with a fork, and the fork is provided with a joint groove for clamping the end part of the piston of the microfluidic chip.

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