CN113546701B - Detection device and detection method - Google Patents
Detection device and detection method Download PDFInfo
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- CN113546701B CN113546701B CN202110842798.4A CN202110842798A CN113546701B CN 113546701 B CN113546701 B CN 113546701B CN 202110842798 A CN202110842798 A CN 202110842798A CN 113546701 B CN113546701 B CN 113546701B
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- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
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Abstract
The invention discloses a detection device and a detection method, wherein the detection device comprises a loading mechanism, a puncture plugging mechanism, an aluminum bubble extrusion mechanism and an extrusion stirring mechanism, wherein the loading mechanism is used for loading a micro-fluidic chip, and the puncture plugging mechanism is used for puncturing aluminum bubbles on the micro-fluidic chip and controlling the conduction or the disconnection of a flow channel on the micro-fluidic chip; the aluminum bubble extrusion mechanism is used for extruding the solution in the punctured aluminum bubble to the outside; the extrusion stirring mechanism is used for stirring the solution in the reaction chamber on the microfluidic chip and extruding the solution after stirring to the outside. The invention can automatically realize the operation of the microfluidic chip to detect whether the target analyte exists or not and improve the detection efficiency.
Description
Technical Field
The invention relates to the technical field of microfluidic chips, in particular to a detection device capable of operating a microfluidic chip to detect whether a target analyte exists and a detection method for detecting whether the target analyte exists by using the detection device.
Background
In recent years, the development, application and updating of in vitro diagnostic instruments and reagents are promoted by the rise and fusion of various new technologies and methods. With the development of chip substrate material science and microchannel fluid moving technology, the microfluidic technology has made great progress. The micro-fluidic chip can integrate a series of basic operation units related to sample preparation, reaction, separation, detection and the like in the fields of chemistry, biology and the like on a micron-sized chip, micro-sized micro-channels and chambers which are arranged in a certain rule are arranged on the micro-fluidic chip, different reagents are released according to a certain sequence and flow into a specified chamber through different micro-channels to complete specified biochemical reaction, so that the purposes of sample preparation, detection and the like are realized. Currently, a microfluidic chip includes a substrate and a plurality of aluminum bubbles, and the aluminum bubbles store reaction reagents required for diagnosis. At present, a microfluidic chip generally includes a substrate, and a plurality of aluminum bubbles, a reaction chamber and a detection chamber, which are disposed on the substrate, wherein the aluminum bubbles store a reaction reagent required for diagnosis, the reaction chamber is used for the reaction reagent and a sample to perform certain operations, such as stirring and mixing, and the detection chamber is used for detecting whether a target analyte exists. At present, a series of operations such as extrusion of a reaction reagent in an aluminum bubble and stirring treatment of a solution in a reaction chamber are often realized manually, and the manual labor cannot be repeated, so that the working efficiency is relatively low, and therefore, a detection device is urgently needed, and the operation of a microfluidic chip can be automatically realized to detect whether a target analyte exists.
Disclosure of Invention
The invention aims to provide a detection device which can automatically realize the operation of a microfluidic chip so as to detect whether a target analyte exists and a detection method for detecting whether the target analyte exists by using the detection device.
To achieve the above object, the present invention provides a variety of detecting devices, which comprise
The loading mechanism comprises a base plate, a pressing plate and a pressing plate driving mechanism connected with the pressing plate, wherein the base plate is provided with an accommodating groove, the pressing plate driving mechanism comprises a push-pull piece, a driving piece, a first connecting piece and a transmission piece, the push-pull piece is arranged on the base plate in a sliding mode and can move along the axial direction of the base plate, the driving piece is arranged on the base plate in a rotating mode, one end of the first connecting piece is connected with the push-pull piece in a rotating mode, the opposite end of the first connecting piece is connected with the driving piece in a rotating mode, and the transmission piece is connected with the pressing plate in a meshing mode and is connected with the driving piece;
the puncture plugging mechanism is arranged on the substrate and is used for puncturing the aluminum bubbles on the micro-fluidic chip and controlling the conduction or the shutoff of a flow channel on the micro-fluidic chip;
the aluminum bubble extrusion mechanism is arranged on the substrate and is used for extruding the solution in the punctured aluminum bubble to the outside;
and the extrusion stirring mechanism is arranged on the substrate and used for stirring the solution in the reaction chamber on the microfluidic chip and extruding the solution after stirring treatment to the outside.
Preferably, the loading mechanism further comprises a locking assembly, the locking assembly comprises a locking piece, a rotating piece and a rotary driving mechanism, a first locking groove is formed in the locking piece, a first locking protrusion which is matched with the first locking groove to realize locking is formed in the rotating piece, the rotating piece is connected with the rotary driving mechanism and rotates under the action of the rotary driving mechanism, and the first locking protrusion extends into the first locking groove or is separated from the first locking groove.
Preferably, the loading device further comprises a first limit component, the first limit component comprises a first limit switch and a first trigger matched with the first limit switch, the first limit switch is arranged on the substrate, and the first trigger is arranged on the push-pull piece.
Preferably, still be equipped with at least one positioning mechanism who is used for fixing a position micro-fluidic chip on the inner wall of storage tank, positioning mechanism includes first elastic component and setting element, the inner wall of storage tank is equipped with the holding hole, the downthehole assembly of holding first elastic component and setting element, the setting element with first elastic component looks butt, just the setting element is in the effect part of first elastic component stretches into in the storage tank.
Preferably, the puncture sealing mechanism comprises:
the mounting structure comprises a first mounting seat, a second mounting seat and a connecting piece, wherein the first mounting seat is provided with at least one first mounting hole and at least one second mounting hole;
each puncture mechanism comprises a puncture assembly and a puncture driving mechanism, each puncture assembly is arranged in a first mounting hole, and each puncture driving mechanism is arranged on the side surface of the first mounting seat and connected with the corresponding puncture assembly;
each plugging mechanism comprises a plugging component and a plugging driving mechanism, each plugging component is installed in a second installation hole, and each plugging driving mechanism is arranged on the side face of the first installation seat and connected with the corresponding plugging component.
Preferably, the lancing assembly comprises:
the first screw rod is arranged in the first mounting hole;
the first nut is arranged in the first mounting hole, sleeved outside the first screw rod, connected with the first screw rod in a threaded manner, connected with the puncture driving mechanism and capable of rotating relative to the first mounting seat under the action of the puncture driving mechanism;
and the pushing piece is connected with the first screw rod and is provided with a sharp part.
Preferably, the occlusion assembly comprises:
the second screw rod is arranged in the second mounting hole;
the second nut is arranged in the second mounting hole, sleeved outside the second screw rod, in threaded connection with the second screw rod, connected with the plugging driving mechanism and capable of rotating relative to the first mounting seat under the action of the plugging driving mechanism;
and the plugging piece is connected with the second screw rod and is provided with a flexible plug.
Preferably, the aluminum bulb extruding mechanism comprises
The second mounting seat is provided with a third mounting hole;
the third screw rod is arranged in the third mounting hole;
the third nut is sleeved outside the third screw rod and is in threaded connection with the third screw rod, and the third nut can rotate relative to the second mounting base;
the first extrusion driving mechanism is arranged on the side surface of the second mounting seat and is meshed and connected with the third nut;
and the first extrusion piece is connected with the third screw rod.
Preferably, the extruding and stirring mechanism includes:
a supporting bracket;
at least one stirring component, wherein each stirring component is rotatably arranged on the bearing bracket;
the stirring driving mechanism is connected with the stirring assembly;
at least one extrusion mechanism, each extrusion mechanism corresponds a stirring subassembly, and each extrusion mechanism includes:
the third mounting seat is provided with a fourth mounting hole;
the fourth screw rod is arranged in the fourth mounting hole;
the fourth nut is sleeved outside the fourth screw rod and is in threaded connection with the fourth screw rod, and the fourth nut can rotate relative to the third mounting seat;
the second extrusion driving mechanism is arranged on the side surface of the third mounting seat and is meshed with the fourth nut;
and the second extrusion piece is connected with the fourth screw rod.
The invention also discloses a detection method, which comprises the following steps:
the push-pull piece is pushed, the push-pull piece drives the driving piece to rotate through the first connecting piece, the driving piece drives the driving piece meshed with the push-pull piece to move, the distance between the pressing plate and the substrate is increased, the microfluidic chip is further assembled into the accommodating groove, the push-pull piece is pulled, the push-pull piece drives the driving piece to rotate again through the first connecting piece, the driving piece drives the driving piece meshed with the push-pull piece to move, the distance between the pressing plate and the substrate is reduced, and after the push-pull piece moves in place, the pressing plate presses the microfluidic chip;
after the micro-fluidic chip is loaded in place, the puncture plugging mechanism punctures the aluminum bubbles on the micro-fluidic chip; the aluminum bubble extrusion mechanism extrudes the aluminum bubbles on the microfluidic chip so as to extrude the solution in the aluminum bubbles to the outside; the puncture plugging mechanism controls the conduction or the disconnection of a flow passage on the microfluidic chip to enable the solution to enter the reaction chamber; the extrusion stirring mechanism stirs the solution in the reaction chamber on the microfluidic chip and extrudes the solution after stirring treatment to the outside into the detection chamber so as to detect whether the target analyte exists.
The invention has the beneficial effects that:
the invention can replace manual work, automatically realize the operation of the microfluidic chip to detect whether the target analyte exists or not, and improve the detection efficiency.
Drawings
FIG. 1 is a schematic perspective view of a detection apparatus according to an embodiment of the present invention;
FIG. 2 is a perspective view of the loading mechanism of FIG. 1;
FIG. 3 is an enlarged schematic view of portion A of FIG. 2;
FIG. 4 is a perspective view of the substrate of FIG. 1;
FIG. 5 is a schematic perspective view of the puncture sealing mechanism of FIG. 1;
FIG. 6 is a schematic cross-sectional view of the lancing mechanism of FIG. 5;
FIG. 7 is a schematic cross-sectional view of the plugging mechanism of FIG. 5;
FIG. 8 is a schematic cross-sectional view of the aluminum bulb pressing mechanism of FIG. 1;
FIG. 9 is a schematic perspective view of the stirring and extruding mechanism of FIG. 1;
FIG. 10 is a cross-sectional schematic view of the pressing mechanism of FIG. 9;
fig. 11 is a perspective view of the agitation assembly and agitation drive mechanism of fig. 9.
Detailed Description
The technical solution of the embodiment of the present invention will be clearly and completely described below with reference to the accompanying drawings of the present invention.
Referring to fig. 1 to 11, a detection apparatus according to an embodiment of the present invention includes a loading mechanism 10, a piercing and plugging mechanism 20, an aluminum bubble extruding mechanism 30, and an extruding and stirring mechanism 40. The loading mechanism 10 is used for loading the microfluidic chip, that is, the microfluidic chip can be installed and fixed in the detection device through the loading mechanism 10; the puncture plugging mechanism 20 is used for puncturing the aluminum bubbles on the microfluidic chip and controlling the flow channels on the microfluidic chip to be connected or disconnected so as to control the flow of the solution; the aluminum bubble extrusion mechanism 30 is used for extruding the aluminum bubbles on the microfluidic chip, and extruding the solution in the punctured aluminum bubbles to the outside so as to extrude the solution in the aluminum bubbles to the outside; the extrusion stirring mechanism 40 is used for stirring the solution in the reaction chamber on the microfluidic chip and extruding the stirred solution out of the reaction chamber.
Specifically, the loading mechanism 10 includes a substrate 11 and a pressing mechanism, wherein the substrate 11 is provided with a containing groove 11a for containing a microfluidic chip, and the microfluidic chip can be assembled in the containing groove 11 a. The pressing mechanism is used for pressing the microfluidic chip in the accommodating groove 11a to prevent the microfluidic chip from moving or shaking and comprises a pressing plate 12 and a pressing plate driving mechanism, the pressing plate 12 and the substrate 11 are oppositely arranged and connected with the driving mechanism, the pressing plate can move towards the direction close to the substrate 11 under the action of the driving mechanism, and the pressing of the microfluidic chip can be realized after the pressing plate moves in place.
Referring to fig. 1 to 4, the pressing plate driving mechanism includes a pushing and pulling member 13, a driving member 14, a first connecting member 15 and a transmission member 16, wherein the pushing and pulling member 13 is slidably disposed on the substrate 11 and is capable of moving in an axial direction of the substrate 11; the driving part 14 is rotatably arranged on the substrate 11, and a driving tooth part 14a is arranged on the driving part 14; one end of the first connecting piece 15 is connected with the driving piece 14, and the opposite end is connected with the push-pull piece 13; the transmission element 16 is connected to the pressure plate 12, and the transmission element 16 is provided with a transmission tooth 16a that meshes with the drive tooth 14 a.
In implementation, a user pushes the push-pull part 13, the drive part 14 is driven to rotate by the first connection part 15 during the moving process of the push-pull part 13, the drive part 14 drives the transmission part 16 engaged with the drive part to move upwards during the rotating process, so that the distance between the pressing plate 12 and the substrate 11 is increased, and at this time, the user can assemble the microfluidic chip into the accommodating groove 11 a. After the microfluidic chip is assembled in place, a user pulls the push-pull part 13 in the opposite direction, the driving part 14 is driven to rotate again by the first connecting part 15 in the moving process of the push-pull part 13, the driving part 14 drives the transmission part 16 meshed with the driving part to move downwards in the rotating process, the distance between the pressing plate 12 and the substrate 11 is further reduced, and after the push-pull part 13 moves in place, the pressing plate 12 presses the microfluidic chip.
As shown in fig. 2 and 3, in order to prevent the push-pull member 13 from being moved by a worker during operation, the loading device further includes a locking assembly 17 for locking the push-pull member 13. The locking assembly 17 comprises a locking piece 17a, a rotating piece 17b and a rotary driving piece 17c, wherein the locking piece 17a is arranged on the push-pull piece 13 and is provided with a first locking groove 17 d; the rotating piece 17b is rotatably arranged on the base plate 11 and is provided with a first locking protrusion 17e which is matched with the first locking groove 17d to realize position locking; the rotary driving member 17c is connected to the rotary member 17b for driving the rotary member 17b to rotate for locking or unlocking. In operation, the rotary driving member 17c drives the rotary member 17b to rotate, so that the first locking protrusion 17e is separated from the first locking groove 17d, thereby unlocking the push-pull member 13. When the operator assembles the microfluidic chip in the containing groove 11a, the push-pull member 13 is further pulled. After the push-pull member 13 is pulled to the right position, the rotary driving member 17c drives the rotary member 17b to rotate, so that the first locking protrusion 17e extends into the first locking groove 17d, the position of the push-pull member 13 is locked, and the push-pull member 13 is prevented from moving.
Further, in order to precisely control the moving stroke of the push-pull member 13, the loading mechanism 10 further includes a first limit component 18, and the first limit component 18 includes a first limit switch 18a and a first trigger 18b matched with the first limit switch 18 a. The first limit switch 18a is disposed on the substrate 11, and the first trigger 18b is disposed on the push-pull member 13, but in other embodiments, the first limit switch 18a may be disposed on the push-pull member 13, and the first trigger 18b may be disposed on the substrate 11, and may be selected according to actual requirements.
As shown in fig. 4, at least one elastic pressing member 11b is disposed in the accommodating groove 11a, and the elastic pressing member 11b is used for pressing the microfluidic chip, so as to ensure that the microfluidic chip is kept stable in the accommodating groove 11a and prevent the microfluidic chip from moving due to the force applied in the accommodating groove 11 a.
Furthermore, at least one positioning mechanism 19 is further arranged in the containing groove 11a, and the positioning mechanism 19 is used for positioning the microfluidic chip, so that the assembly precision of the microfluidic chip is improved, and the subsequent process treatment is facilitated. The positioning mechanism 19 includes a first elastic member (not shown) and a positioning member 19a, an accommodating hole is formed on an inner wall of the accommodating groove 11a, the first elastic member and the positioning member 19a are both assembled in the accommodating hole, one end of the first elastic member abuts against a groove wall of the accommodating hole, an opposite end of the first elastic member abuts against the positioning member 19a, the positioning member 19a extends out of the accommodating hole at an action portion of the first elastic member, and an extended portion of the positioning member 19a is located in the accommodating groove 11 a. In this embodiment, the positioning element 19a is a ball or a positioning column, and when the positioning element 19a is a positioning column, an arc-shaped guide surface is arranged at an end portion of the positioning column, which is located in the accommodating hole, and the arc-shaped guide surface can reduce friction force between the positioning column and the microfluidic chip on one hand, and is convenient for the positioning column to be separated from a positioning groove on the microfluidic chip on the other hand, so that the assembly efficiency is improved.
Further, at least one pressing protrusion (not shown) is disposed on an end surface of the pressing plate 12 facing the accommodating groove 11a, and the pressing protrusion is used for pressing and fixing the microfluidic chip in the accommodating groove 11 a. In implementation, after the pressing plate 12 is moved in place, the pressing convex part on the pressing plate 12 contacts with the microfluidic chip to press and fix the microfluidic chip.
As shown in fig. 5 to 7, the puncture sealing mechanism 20 includes a first mounting seat 21, at least one puncture mechanism 22 and at least one sealing mechanism 23. The first mounting seat 21 is fixed on the base plate 11, the pressure plate 12 is located between the base plate 11 and the first mounting seat 21, at least one first mounting hole 21a and at least one second mounting hole 21b are formed in the first mounting seat 21, the first mounting hole 21a is used for mounting the puncturing mechanism 22, and the second mounting hole 21b is used for mounting the plugging mechanism 23. The puncture mechanism 22 includes a puncture component and a puncture driving mechanism, the puncture component is installed in a first installation hole 21a, and the puncture driving mechanism is installed on the side surface of the first installation seat 21, connected with the puncture component, and used for driving the puncture component to puncture the aluminum bubbles on the microfluidic chip. The plugging mechanism 23 includes a plugging component and a plugging driving mechanism, the plugging component is disposed in a second mounting hole 21b, and the plugging driving mechanism is also disposed on a side surface of the first mounting seat 21, connected to the plugging component, and configured to drive the plugging component to perform plugging processing on a flow channel on the microfluidic chip, and control the flow of a solution. The puncturing driving mechanism and the plugging driving mechanism are arranged on the side surface of the first mounting seat 21, so that the space occupied by the puncturing and plugging device in the vertical direction is reduced, and the installation of other parts is facilitated.
Referring to fig. 6 to 7, the puncturing assembly includes a first lead screw 22a, a first nut 22b and a pushing member 22c, wherein the first lead screw 22a and the first nut 22b are both assembled in the first mounting hole 21a, the first lead screw 22a is connected to the pushing member 22c, the first nut 22b is sleeved outside the first lead screw 22a and is in threaded connection with the first lead screw 22a, the first nut 22b is rotatable relative to the first mounting seat 21, the first nut 22b converts a rotational motion into a linear motion of the first lead screw 22a during a rotation process to drive the pushing member 22c to move, so as to puncture an aluminum bubble on the microfluidic chip, and a solution in the aluminum bubble can flow out to the outside after puncturing for subsequent use.
Further, first nut 22b includes a first body 22d and a first bevel gear 22e, and first body 22d and first bevel gear 22e are preferably integrally formed to improve the stability of first nut 22 b.
Further, the pushing member 22c is provided with a sharp portion 22f for piercing the aluminum bulb. Meanwhile, the pushing piece 22c and the first screw rod 22a are in a non-collinear design, namely a staggered design, so that a certain yielding space can be provided for installation of other parts without increasing the volume of the puncture plugging device.
Further, in order to avoid the damage of the microfluidic chip caused by the excessive force applied to the pushing member 22c, the pushing member 22c is elastically connected to the first lead screw 22a, that is, the pushing member 22c is connected to the first lead screw 22a through the first elastic buffer component, so as to avoid the damage of the microfluidic chip. Specifically, the first elastic buffer assembly includes a second connecting member 22g and a second elastic member (not shown in the figures), the second connecting member 22g is provided with a first receiving hole (not shown in the figures), the pushing member 22c can be partially received in the first receiving hole, and the pushing member 22c is provided with a first limiting portion; the second elastic member is at least partially accommodated in the first accommodating hole, is sleeved outside the pushing member 22c, and has one end abutting against the first limiting portion and the opposite end abutting against the inner wall of the first accommodating hole. In practice, after the pushing element 22c abuts against a needle (not shown) on the microfluidic chip, a reaction force is applied to the pushing element 22c, and the reaction force causes the pushing element 22c to press the second elastic element, so that the pushing element 22c can extend into the first receiving hole, thereby preventing the damage to the microfluidic chip.
As shown in fig. 6, the lancing drive mechanism includes a second bevel gear 22h and a first power source 22j, wherein the second bevel gear 22h is engaged with the first bevel gear 22e, the first power source 22j is connected to the second bevel gear 22h, and the first power source 22j drives the first bevel gear 22e to rotate via the second bevel gear 22h, thereby rotating the first nut 22 b. In the present embodiment, the first power source 22j is preferably an electric motor.
Further, in order to accurately control the moving position of the first lead screw 22a, the puncture driving mechanism further comprises a second limit switch 22k, and a second trigger 22m matched with the second limit switch 22k is arranged on the second lead screw 23 a. In implementation, when the second trigger 22m moves to the position of the second limit switch 22k, the second trigger 22m can trigger the second limit switch 22k, so that the second limit switch 22k outputs a control signal to the control system, and the control system receives the control signal and then controls the first power source 22j to stop working, so as to accurately control the moving position of the first lead screw 22 a. The second limit switch 22k is preferably a proximity switch, although in other implementations, limit switches that perform the same function may be selected according to actual requirements.
In this embodiment, the position of the second trigger 22m on the first lead screw 22a is adjustable to adapt to different application scenarios, for example, in some application scenarios, the distance that the first lead screw 22a needs to move is longer, and in some application scenarios, the distance that the first lead screw 22a needs to move is shorter, so that the moving stroke of the first lead screw 22a can be controlled by adjusting the position of the second trigger 22 m.
Referring to fig. 5 and 7, the blocking assembly includes a second lead screw 23a, a second nut 23b and a blocking piece 23c, wherein the second lead screw 23a and the second nut 23b are both assembled in the second mounting hole 21b, the second lead screw 23a is connected to the blocking piece 23c, the second nut 23b is sleeved outside the second lead screw 23a and is in threaded connection with the second lead screw 23a, the second nut 23b can rotate relative to the first mounting seat 21, and the second nut 23b converts the rotational motion into the linear motion of the second lead screw 23a during the rotation process to drive the blocking piece 23c to move, so as to block the flow channel on the microfluidic chip and control the flow of the solution.
Further, the second nut 23b includes a second body 23d and a third bevel gear 23e, and the second body 23d and the third bevel gear 23e are preferably integrally formed to improve stability of the second nut 23 b.
Further, a flexible plug 23f for plugging the flow channel is arranged on the plugging member 23c, the plug is preferably made of a flexible material to prevent the solution from leaking in the plugging process, and the flexible material is rubber and the like. Meanwhile, the blocking piece 23c and the second screw rod 23a are in a non-collinear design, namely a staggered design, so that a certain yielding space can be provided for installation of other parts without increasing the volume of the puncture blocking device.
Further, in order to avoid the blocking piece 23c from damaging the microfluidic chip due to excessive force, the blocking piece 23c is elastically connected to the second lead screw 23a, that is, the blocking piece 23c is connected to the second lead screw 23a through the second elastic buffer assembly, so as to avoid the damage to the microfluidic chip. Specifically, the second elastic buffer assembly includes a third connecting member 23g and a third elastic member (not shown in the figure), the third connecting member 23g is provided with a second receiving hole, the blocking member 23c can be partially received in the second receiving hole, the blocking member 23c is provided with a second limiting portion, the third elastic member is at least partially received in the second receiving hole, and is sleeved outside the blocking member 23c, and one end of the third elastic member abuts against the second limiting portion, and the opposite end abuts against the inner wall of the second receiving hole. During implementation, after the blocking piece 23c is abutted to the flow channel of the microfluidic chip, the blocking piece 23c is subjected to a reaction force, the reaction force enables the blocking piece 23c to extrude the third elastic piece, and the blocking piece 23c can extend into the second accommodating hole so as to avoid damage to the microfluidic chip.
As shown in fig. 7, the plugging driving mechanism includes a fourth bevel gear 23h and a second power source 23j, wherein the fourth bevel gear 23h is engaged with the third bevel gear 23e, the second power source 23j is connected to the fourth bevel gear 23h, and the second power source 23j drives the third bevel gear 23e to rotate through the fourth bevel gear 23h, so as to rotate the second nut 23 b. In the present embodiment, the second power source 23j is preferably a motor.
Further, in order to accurately control the moving position of the second screw 23a, the plugging driving mechanism further comprises a third limit switch 23k, and a third trigger 23m matched with the third limit switch 23k is arranged on the second screw 23 a. In implementation, when the third trigger 23m moves to the position of the third limit switch 23k, the third trigger 23m can trigger the third limit switch 23k, so that the third limit switch 23k outputs a control signal to the control system, and the control system receives the control signal and then controls the second power source 23j to stop working, so as to accurately control the moving position of the second screw rod 23 a. The third limit switch 23k is preferably a proximity switch, and in other embodiments, a limit switch capable of achieving the same function may be selected according to actual requirements.
In this embodiment, the position of the third trigger 23m on the second lead screw 23a is adjustable to adapt to different application scenarios, for example, in some application scenarios, the distance that the second lead screw 23a needs to move is longer, and in some application scenarios, the distance that the second lead screw 23a needs to move is shorter, so that the moving stroke of the second lead screw 23a can be controlled by adjusting the position of the third trigger 23 m.
As shown in fig. 8, the aluminum bulb extruding mechanism 30 includes a second mount 31, a third lead screw 32, a third nut 33, a first extruding drive mechanism 34, and a first extruding member 35. The second mounting seat 31 is provided with a third mounting hole 31a extending in the longitudinal direction (vertical direction shown in fig. 8). The third screw 32 and the third nut 33 are both assembled in the third mounting hole 31a, the third screw 32 is connected with the first extrusion piece 35, the third nut 33 is sleeved outside the third screw 32, the third nut 33 is in threaded connection with the third screw 32, and the third nut 33 can rotate relative to the second mounting seat 31. In practice, the third nut 33 can be mounted in the third mounting hole 31a through a bearing and can rotate relative to the second mounting seat 31 under the action of the bearing. The third nut 33 converts the rotation into the linear motion of the third screw 32 during the rotation, so that the third screw 32 can drive the first extrusion 35 to move.
The first pressing driving mechanism 34 is installed on a side surface of the second installation seat 31 and connected to the third nut 33 in the third installation hole 31a to drive the third nut 33 to rotate. By providing the first pressing drive mechanism 34 on the side of the second mounting seat 31, the space occupied by the aluminum bulb pressing mechanism 30 in the longitudinal direction can be reduced to facilitate the mounting of other parts and/or mechanisms.
As shown in fig. 8, the third nut 33 includes a third body 33a and a fifth bevel gear 33b, the fifth bevel gear 33b is connected to the first pressing drive mechanism 34, the first pressing drive mechanism 34 drives the third nut 33 to integrally rotate through the fifth bevel gear 33b, and the third screw 32 is driven to move during the rotation of the third nut 33. In this embodiment, the third body 33a and the fifth bevel gear 33b are integrally formed, but in other embodiments, the third body 33a and the fifth bevel gear 33b may also be separately designed, and the two are integrally connected by a corresponding connecting member, such as a nut.
As shown in fig. 8, the first pressing drive mechanism 34 includes a fifth bevel gear 34a and a third power source 34b for driving the fifth bevel gear 34a to rotate, the fifth bevel gear 34a is engaged with the fifth bevel gear 33b, and the third power source 34b is connected to the fifth bevel gear 34 a. In implementation, the third power source 34b drives the fifth bevel gear 34a to rotate, the fifth bevel gear 34a drives the third nut 33 to rotate through the fifth bevel gear 33b engaged with the fifth bevel gear, the third nut 33 further drives the third screw rod 32 to move, and the third screw rod 32 drives the first extrusion piece 35 to move to the position of the aluminum bubble, so as to extrude the aluminum bubble and extrude the solution in the aluminum bubble to the outside. In the present embodiment, the third power source 34b is preferably a rotating electric machine.
As shown in fig. 8, the first extruding member 35 has an overall cylindrical structure, and the end surface contacting with the aluminum bulb is outwardly protruded to form an arc-shaped extruding surface. The solution in the aluminum bubble can be completely extruded by arranging the arc-shaped extrusion surface. In this embodiment, the first extrusion member 35 is preferably made of a flexible material to avoid damage to the aluminum bubbles during the extrusion process.
As shown in fig. 8, in order to accurately control the moving stroke of the screw rod body, the aluminum bulb extruding mechanism 30 further includes a fourth limit switch 36, and a fourth trigger 37 matched with the fourth limit switch 36 is arranged on the third screw rod 32. The cooperation here means that when the fourth trigger 37 triggers the fourth limit switch 36, the fourth limit switch 36 outputs an active signal to the control system. In order to make the stroke of the third screw 32 controllable to adapt to different application scenarios, for example, in some application scenarios, the distance that the third screw 32 needs to move is longer, and in some application scenarios, the distance that the third screw 32 needs to move is shorter, so the stroke of the movement of the third screw 32 can be controlled by adjusting the position of the fourth trigger 37, that is, the position of the fourth trigger 37 on the third screw 32 is adjustable. The first extrusion driving mechanism 34 is disposed on the side of the second mounting seat 31, so as to reduce the space occupied by the aluminum bubble extrusion mechanism 30 in the longitudinal direction, and facilitate the installation of other components and/or mechanisms.
As shown in fig. 9 to 11, the pressing and stirring mechanism 40 includes a support bracket 41, at least one stirring member 42, a stirring driving mechanism 43, and at least one pressing mechanism 44. Wherein, the supporting bracket 41 is used for mounting the stirring component 42 and the stirring driving mechanism 43; each stirring component 42 corresponds to one reaction chamber, and each stirring component 42 is arranged on the bearing support 41 and is used for stirring the solution in the reaction chamber; the stirring driving mechanism 43 is connected with each stirring assembly 42 and is used for driving the stirring assemblies 42 to stir the solution in the reaction chamber; each of the pressing mechanisms 44 corresponds to one of the stirring assemblies 42, and the pressing mechanism 44 is disposed above (in the direction shown in fig. 1) the corresponding stirring assembly 42 to press the reaction chamber through the corresponding stirring assembly 42, so that the solution in the reaction chamber flows out to the outside.
As shown in fig. 3, each stirring assembly 42 includes a rotating base 42a, a stirring column 42b and a fourth elastic member 42c, wherein a fitting hole extending along the axial direction is formed on the rotating base 42a, the rotating base 42a is sleeved outside the stirring column 42b through the fitting hole, and a second locking groove (not shown) extending along the axial direction is formed on a hole wall of the rotating base 42 a; the mixing column 42b is movable in the axial direction in the rotating base 42a, one end of the mixing column is provided with a third limiting part 42d, the opposite end is provided with a magnetic part 42e, the mixing column 42b is further provided with a second locking protrusion (not shown) which extends into the second locking groove and is used for enabling the mixing column 42b and the rotating base 42a to rotate synchronously; the fourth elastic member 42c is sleeved outside the stirring rod 42b, and is preferably located between the third limiting portion 42d and the rotating base 42a, one end of the fourth elastic member 42c abuts against the third limiting portion 42d, and the opposite end abuts against the rotating base 42a, in practice, a limiting convex ring may be disposed on the inner wall of the rotating base 42a, so that the fourth elastic member 42c abuts against the inner wall of the rotating base 42a, the fourth elastic member 42c is preferably a spring, and of course, an elastic sheet and the like may be selected. Under the initial condition, the protruding second locking that is located the second locking groove all the time of second locking on the stirs 42b under the effect of fourth elastic component 42c, under the cooperation between them, rotate base 42a pivoted in-process and can drive stirs 42b synchronous rotation, stirs 42b and rotates the in-process and can drive magnetism portion 42e of inhaling and rotate, magnetism portion 42e drives the magnetic part rotation that corresponds in the reacting chamber to the realization is to the stirring of solution in the reacting chamber.
Further, the magnetic attraction portion 42e includes a flexible contact member connected to the stirring rod 42b and a magnet disposed inside the flexible contact member, and the flexible contact member is made of a flexible material, which includes but is not limited to rubber. The magnet inside the flexible contact element and the magnetic element in the reaction chamber are attracted in opposite directions, so that the magnetic part is driven to rotate when the magnetic part rotates, and the stirring treatment of the solution in the reaction chamber is realized. The magnetic part is made of flexible materials, so that the damage to the reaction chamber caused by subsequent extrusion of the reaction chamber can be avoided.
Further, in order to facilitate the magnetic attraction part 42e to extrude the solution in the reaction chamber, an arc-shaped contact surface is further provided on the end surface of the flexible contact member.
In this embodiment, two stirring assemblies 42 are disposed on the supporting bracket 41, and the two stirring assemblies 42 are engaged with each other, that is, in each stirring assembly 42, a tooth portion 42f is disposed on a side surface of the rotating base 42a, and the two rotating bases 42a are engaged with each other through the tooth portion 42 f. In other embodiments, more than two stirring assemblies 42 may be disposed on the support bracket 41, and multiple stirring assemblies 42 are engaged with each other in sequence, however, one stirring assembly 42 may also be disposed on the support bracket 41, and the number of the stirring assemblies 42 may be set according to actual requirements.
As shown in fig. 11, the stirring driving mechanism 43 includes a transmission gear 43a and a gear driving mechanism 43b for driving the transmission gear 43a to rotate, the gear driving mechanism 43b is connected with the transmission gear 43a, and the gear driving mechanism 43b is preferably a motor. When a stirring member 42 is provided on the support bracket 41, the transmission gear 43a is directly engaged with the tooth portion 42f of the rotation base 42a of the stirring member 42. When a plurality of stirring units 42 are provided on the support frame 41, the transmission gear 43a is engaged with the tooth portion 42f of the rotation base 42a of any one of the stirring units 42.
As shown in fig. 10, the pressing mechanism 44 includes a third mount 44a, a fourth lead screw 44b, a fourth nut 44c, a second pressing drive mechanism 44d, and a second pressing member 44 e. The third mounting seat 44a is provided with a fourth mounting hole 44f extending along the axial direction thereof. The fourth screw 44b and the fourth nut 44c are disposed in the fourth mounting hole 44f, the fourth nut 44c is sleeved outside the fourth screw 44b, the fourth screw and the fourth nut are connected by threads, the fourth nut 44c can rotate relative to the third mounting seat 44a, and the fourth nut 44c drives the fourth screw 44b to move along the axial direction of the third mounting seat 44a in the rotating process, that is, the rotary motion of the fourth nut 44c is converted into the linear motion of the fourth screw 44b, so that the fourth screw 44b drives the second extruding member 44e to move.
In this embodiment, the fourth nut 44c includes a fourth body 44g and a seventh bevel gear 44h, the seventh bevel gear 44h is connected to the second pressing driving mechanism 44d, the second pressing driving mechanism 44d drives the fourth nut 44c to integrally rotate through the seventh bevel gear 44h, and the fourth screw 44b is driven to linearly move during the rotation of the fourth nut 44 c. The fourth body 44g and the seventh bevel gear 44h are integrally formed, however, in other embodiments, the fourth body 44g and the seventh bevel gear 44h may also be separately designed, and the connection between the fourth body and the seventh bevel gear is realized by a corresponding first connecting member 15, such as a nut.
As shown in fig. 9 and 10, the second pressing driving mechanism 44d is disposed on a side surface of the third mounting seat 44a, and includes an eighth bevel gear 44j and a fourth power source 44k for driving the eighth bevel gear 44j to rotate, the eighth bevel gear 44j is engaged with the seventh bevel gear 44h, the fourth power source 44k is connected to the eighth bevel gear 44j, and the fourth power source 44k is preferably a motor. By arranging the second extrusion driving mechanism 44d on the side surface of the third mounting seat 44a, the occupied space of the extrusion stirring mechanism in the axial direction can be reduced, so that the installation of other parts and/or mechanisms is facilitated, and the overall volume of the detection device is reduced. In practice, the fourth power source 44k drives the eighth bevel gear 44j to rotate, the eighth bevel gear 44j drives the fourth nut 44c to rotate through the seventh bevel gear 44h engaged with the eighth bevel gear, the fourth nut 44c further drives the fourth lead screw 44b to move, and the fourth lead screw 44b drives the second extrusion piece 44e to move to the position of the reaction chamber, so as to extrude the solution in the reaction chamber.
As shown in fig. 9 and 10, in order to accurately control the moving stroke of the screw rod body, the pressing mechanism 44 further includes a fifth limit switch 44m, and a fifth trigger 44n matched with the fifth limit switch 44m is disposed on the fourth screw rod 44 b. When the fifth trigger 44n triggers the fifth limit switch 44m, the fifth limit switch 44m outputs an effective signal to the control system, and the control system further controls the power source to stop working. Further, in order to make the stroke of the fourth lead screw 44b controllable to adapt to different application scenarios, for example, in some application scenarios, the distance that the fourth lead screw 44b needs to move is longer, and in some application scenarios, the distance that the fourth lead screw 44b needs to move is shorter, so that the stroke of the fourth lead screw 44b can be controlled by adjusting the position of the fifth trigger 44n, that is, the position of the fifth trigger 44n on the fourth lead screw 44b is adjustable.
The working principle of the detection device is as follows:
the user pushes the push-pull part 13, the push-pull part 13 drives the driving part 14 to rotate through the first connecting part 15 in the moving process, the driving part 14 drives the driving part 16 meshed with the driving part to move upwards in the rotating process, the distance between the pressing plate 12 and the substrate 11 is further increased, and at the moment, the user can assemble the microfluidic chip into the accommodating groove 11 a. After the microfluidic chip is assembled in place, a user pulls the push-pull part 13 in the opposite direction, the driving part 14 is driven to rotate again by the first connecting part 15 in the moving process of the push-pull part 13, the driving part 14 drives the transmission part 16 meshed with the driving part to move downwards in the rotating process, the distance between the pressing plate 12 and the substrate 11 is further reduced, and after the push-pull part 13 moves in place, the pressing plate 12 presses the microfluidic chip. After the push-pull member 13 is pulled to the right position, the rotary driving member 17c drives the rotary member 17b to rotate, so that the first locking protrusion 17e extends into the first locking groove 17d, the position of the push-pull member 13 is locked, and the push-pull member 13 is prevented from moving.
After the micro-fluidic chip is mounted in place, the puncture driving mechanism drives the puncture assembly to puncture the aluminum bubbles on the micro-fluidic chip, and the aluminum bubble extrusion mechanism 30 extrudes the aluminum bubbles on the micro-fluidic chip to extrude the solution in the aluminum bubbles to the outside. The plugging driving mechanism drives the plugging assembly to plug a flow channel on the microfluidic chip, so that the flow of the solution is controlled, and the solution can enter the reaction chamber. The extruding and stirring mechanism 40 further stirs the solution in the reaction chamber on the microfluidic chip and extrudes the stirred solution out of the reaction chamber, so that the mixed solution can enter the detection chamber to detect whether the target analyte exists.
The invention can replace manual work, automatically realize the operation of the microfluidic chip to detect whether the target analyte exists or not, and improve the detection efficiency.
Therefore, the scope of the present invention should not be limited to the disclosure of the embodiments, but includes various alternatives and modifications without departing from the scope of the present invention, which is defined by the claims of the present patent application.
Claims (10)
1. A detection device for operating a microfluidic chip to detect the presence of a target analyte, the detection device comprising
The loading mechanism comprises a base plate, a pressing plate and a pressing plate driving mechanism connected with the pressing plate, wherein the base plate is provided with a containing groove, the pressing plate driving mechanism comprises a push-pull piece, a driving piece, a first connecting piece and a transmission piece, the push-pull piece is arranged on the base plate in a sliding mode and can move along the axial direction of the base plate, the driving piece is arranged on the base plate in a rotating mode, one end of the first connecting piece is connected with the push-pull piece in a rotating mode, the opposite end of the first connecting piece is connected with the driving piece in a rotating mode, and the transmission piece is connected with the pressing plate in a connecting mode and is meshed with the driving piece;
the puncture plugging mechanism is arranged on the substrate and is used for puncturing the aluminum bubbles on the microfluidic chip and controlling the conduction or the disconnection of a flow passage on the microfluidic chip;
the aluminum bubble extrusion mechanism is arranged on the substrate and is used for extruding the solution in the punctured aluminum bubble to the outside;
and the extruding and stirring mechanism is arranged on the substrate and is used for stirring the solution in the reaction chamber on the microfluidic chip and extruding the solution after stirring treatment to the outside.
2. The detecting device for detecting the rotation of the motor rotor according to the claim 1, wherein the loading mechanism further comprises a locking assembly, the locking assembly comprises a locking member, a rotating member and a rotation driving mechanism, the locking member is provided with a first locking groove, the rotating member is provided with a first locking protrusion which is matched with the first locking groove to realize locking, the rotating member is connected with the rotation driving mechanism and rotates under the action of the rotation driving mechanism, and the first locking protrusion extends into the first locking groove or is separated from the first locking groove.
3. The detecting device for detecting the rotation of a motor rotor according to the claim 1, wherein the loading mechanism further comprises a first limit component, the first limit component comprises a first limit switch and a first trigger matched with the first limit switch, the first limit switch is arranged on the base plate, and the first trigger is arranged on the push-pull member.
4. The detection device according to claim 1, wherein at least one positioning mechanism for positioning the microfluidic chip is further disposed on an inner wall of the accommodating groove, the positioning mechanism includes a first elastic member and a positioning member, an accommodating hole is disposed on the inner wall of the accommodating groove, the first elastic member and the positioning member are assembled in the accommodating hole, the positioning member abuts against the first elastic member, and the positioning member partially extends into the accommodating groove under the action of the first elastic member.
5. The detection device of claim 1, wherein the puncture sealing mechanism comprises:
the mounting structure comprises a first mounting seat, a second mounting seat and a connecting piece, wherein the first mounting seat is provided with at least one first mounting hole and at least one second mounting hole;
each puncture mechanism comprises a puncture assembly and a puncture driving mechanism, each puncture assembly is arranged in a first mounting hole, and each puncture driving mechanism is arranged on the side surface of the first mounting seat and connected with the corresponding puncture assembly;
each plugging mechanism comprises a plugging component and a plugging driving mechanism, each plugging component is installed in a second installation hole, and each plugging driving mechanism is arranged on the side face of the first installation seat and connected with the corresponding plugging component.
6. The detection device of claim 5, wherein the lancing assembly comprises:
the first screw rod is arranged in the first mounting hole;
the first nut is arranged in the first mounting hole and sleeved outside the first screw rod, is in threaded connection with the first screw rod, is connected with the puncture driving mechanism and can rotate relative to the first mounting seat under the action of the puncture driving mechanism;
and the pushing piece is connected with the first screw rod and is provided with a sharp part.
7. The detection device of claim 6, wherein the occluding assembly comprises:
the second screw rod is arranged in the second mounting hole;
the second nut is arranged in the second mounting hole and sleeved outside the second screw rod, is in threaded connection with the second screw rod, is connected with the plugging driving mechanism and can rotate relative to the first mounting seat under the action of the plugging driving mechanism;
and the plugging piece is connected with the second screw rod and is provided with a flexible plug.
8. The probe apparatus of claim 7, wherein the aluminum bubble extrusion mechanism comprises
The second mounting seat is provided with a third mounting hole;
the third screw rod is arranged in the third mounting hole;
the third nut is sleeved outside the third screw rod and is in threaded connection with the third screw rod, and the third nut can rotate relative to the second mounting base;
the first extrusion driving mechanism is arranged on the side surface of the second mounting seat and is meshed with the third nut;
and the first extrusion piece is connected with the third screw rod.
9. The detecting device according to claim 8, wherein the squeezing and stirring mechanism comprises:
a supporting bracket;
at least one stirring component, wherein each stirring component is rotatably arranged on the bearing bracket;
the stirring driving mechanism is connected with the stirring component;
at least one extrusion mechanism, every extrusion mechanism corresponds a stirring component, and every extrusion mechanism includes:
the third mounting seat is provided with a fourth mounting hole;
the fourth screw rod is arranged in the fourth mounting hole;
the fourth nut is sleeved outside the fourth screw rod and is in threaded connection with the fourth screw rod, and the fourth nut can rotate relative to the third mounting seat;
the second extrusion driving mechanism is arranged on the side surface of the third mounting seat and is meshed with the fourth nut;
and the second extrusion piece is connected with the fourth screw rod.
10. A detection method based on the detection device according to any one of claims 1 to 9, comprising the steps of:
pushing the push-pull piece, driving the driving piece to rotate by the push-pull piece through the first connecting piece, driving the driving piece meshed with the driving piece to move by the driving piece, so that the distance between the pressing plate and the substrate is increased, further assembling the microfluidic chip into the accommodating groove, pulling the push-pull piece, driving the driving piece to rotate again by the push-pull piece through the first connecting piece, driving the driving piece meshed with the driving piece to move by the driving piece, so that the distance between the pressing plate and the substrate is reduced, and after the push-pull piece moves in place, pressing the pressing plate against the microfluidic chip;
after the micro-fluidic chip is loaded in place, the aluminum bubbles on the micro-fluidic chip are punctured by the puncturing and plugging mechanism; the aluminum bubble extrusion mechanism extrudes the aluminum bubbles on the microfluidic chip so as to extrude the solution in the aluminum bubbles to the outside; the puncture plugging mechanism controls the conduction or the disconnection of a flow passage on the microfluidic chip to enable the solution to enter the reaction chamber; the extrusion stirring mechanism stirs the solution in the reaction chamber on the microfluidic chip and extrudes the solution after stirring treatment to the outside into the detection chamber so as to detect whether the target analyte exists.
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Application publication date: 20211026 Assignee: Serun (Shanghai) Medical Technology Co.,Ltd. Assignor: Green leaf diagnostic product technology (Guangdong) Co.,Ltd. Contract record no.: X2022980009352 Denomination of invention: A detection device and method Granted publication date: 20220624 License type: Common License Record date: 20220705 |
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Effective date of registration: 20230410 Address after: Room A, 7th Floor, Building 65, No. 421 Hongcao Road, Xuhui District, Shanghai, 200000 Patentee after: Serun (Shanghai) Medical Technology Co.,Ltd. Address before: 510700 unit 601, 6th floor, No.8 helix 3 Road, Guangzhou International Biological Island, Huangpu District, Guangzhou City, Guangdong Province Patentee before: Green leaf diagnostic product technology (Guangdong) Co.,Ltd. |