CN207786625U

CN207786625U - Biochemistry detection micro-fluidic chip and biochemistry detection micro-fluidic chip system

Info

Publication number: CN207786625U
Application number: CN201721605116.3U
Authority: CN
Inventors: 丁锐
Original assignee: Shenzhen Hua Yan Micrometer Medical Science & Technology Co Ltd
Priority date: 2017-11-27
Filing date: 2017-11-27
Publication date: 2018-08-31
Anticipated expiration: 2027-11-27

Abstract

The utility model is related to micro-fluidic chip biochemistry detection technical fields, more particularly to biochemistry detection micro-fluidic chip and biochemistry detection micro-fluidic chip system.Chip includes：Sample injection unit (1), reaction member (3), waste unit (4)；Treat quantitative the first dosing unit (5) of sample sheet comprising slit (51), the first sample chamber (52) separated by the first slit and the second sample chamber (53)；First sample chamber is communicated with sample injection unit, and the second sample chamber is communicated with waste unit；There is third to report an error unit (14) between reaction member and waste unit, with chamber, receives the sample to be checked via reaction member.Chip system is by upper, middle and lower up of three layers；Middle level is the chip；Levels are for covering closing middle level；There are the sample holes being connect with sample injection unit, and the relief hole of corresponding liquid storage unit in upper layer.The chip detection process is simple, can be quantitative to reaction sample, and high sensitivity, repeatability are strong.

Description

Biochemistry detection micro-fluidic chip and biochemistry detection micro-fluidic chip system

Technical field

The utility model is related to micro-fluidic chip biochemistry detection technical field, more particularly to a kind of micro-fluidic core of biochemistry detection Piece and a kind of biochemistry detection micro-fluidic chip system.

Background technology

Microfluidic chip technology is otherwise known as Microfluid based Lab on a chip or chip lab, refers at one piece several squares Centimetre chip on the chemistry or biology laboratory that build, it sample preparation involved in the fields such as chemistry and biology, Reaction detaches, detection, cell culture, sorting, and the basic operation units such as cracking are integrated on the chip of one piece of very little, by micro- logical Road forms network, runs through whole system with controlled fluid, chemical to realize biology, the various work(in the fields such as medical diagnosis Energy.

The essential characteristic and sharpest edges of microfluidic chip technology be：A variety of cellular constructions can on small chip platform With flexible combination so that chip design is upper flexible and changeable and multiple functional；The small required detection of chip internal structure unit Sample size is considerably less, and the bigger serface of microstructure unit allows interior reagent quickly to spread to realize fast reaction and inspection It surveys；Micro-fluidic chip is usually automatically performed internal-response by necessary instrument, so during actual test, micro-fluidic chip Technology can reduce the technology requirement to medicine testing staff, reduce the human error of detection, and then reduce the medicine inspection of patient Survey cost；Microfluidic chip technology using automation equipment due to being completed, so can be with so internal-response process is fully controllable It is more accurate to obtain, more sensitive detection data.

In medical science, in-vitro diagnosis is a very important branch, refers in human vitronectin, to deriving from The sample (such as blood, urine, the body fluid such as saliva) of human body is detected to obtain clinical diagnosis information, is sentenced according to the information Whether disconnected subject suffers from certain disease or the diagnostic method for being subjected to the infection of certain germ.Currently, with biotechnology, especially Biochemistry, immunology, the fast development of molecular biology, in-vitro diagnosis mainly have biochemistry according to testing principle and detection method Diagnosis, immunodiagnosis, molecule diagnosis, microbe diagnosis, the diagnosis of blood coagulation class, fluidic cell diagnosis etc., mesophytization is exempted from Epidemic disease, molecule diagnosis are the major component fields of current China's in-vitro diagnosis.

Biochemical diagnosis refers to by enzyme, carbohydrate, lipid, albumen in various biochemical reactions or immunoreaction measurement body With the detection means of the indexs such as nonprotein nitrogen class, inorganic elements class, liver function, it is mainly used in the conventional detection of hospital, is doctor Treat the element of detection.Biochemistry detection is mainly completed by large-scale Biochemical Analyzer at present, such as full-automatic biochemical Analyzer has been realized in entire testing process and (for example samples, reagent adding, mix, as a result heat preservation, colorimetric calculates, obtain report Accuse and etc.) fully integrated and full automation.But existing automatic biochemistry analyzer is expensive, it is bulky, and grasp Make complexity, the time cycle for needing the testing staff of profession to operate, and often detected in hospital is very long, it is difficult to right Patient is used for quickly detecting, and is coped with immediately to disease.

Although the development of biochemistry detection field is more early, the patent that biochemistry detection is carried out using microfluidic chip technology is compared It is few, wherein the patent of multi objective while detection can be carried out with regard to less.It investigates and finds by patent, have in recent years both at home and abroad a small amount of The related patents of biochemistry detection are carried out using microflow control technique, for example a kind of more work(are disclosed in Chinese patent 201110416978 Energy multiple determination integrated chip, but the chip is driven using the mode of centrifugation, needs to carry out high speed to chip Rotation, increases the danger of operation；A kind of multi objective biochemistry detection is disclosed in Chinese patent 201210142337 for another example Micro-fluidic chip, but the chip does not have reagent store function, and the mechanism that do not report an error, so it is difficult to ensure the accurate of detection Property, while chip interior carries out liquid isolation using vapour-pressure type micro-valve door, to realize quantifying for liquid, but this mode exists It is but difficult to accurately control in practical application, the accurate quantitative analysis of liquid is difficult to realize in actual test.

Utility model content

The purpose of this utility model is to overcome problems as above of the existing technology, provide a kind of biochemistry detection miniflow Chip is controlled, which has detection process simple, can be quantified to reaction sample, each structural unit is interconnected The characteristics of, and multiple indexs of sample can be detected simultaneously, detection sensitivity is high, repeatability is strong.

To achieve the goals above, the utility model provides a kind of biochemistry detection micro-fluidic chip, the micro-fluidic chip packet It includes：For receiving the sample injection unit of sample to be checked, providing the reaction member of reacting environment for the sample to be checked and reaction reagent And the waste unit for collecting waste liquid；The micro-fluidic chip further includes for carrying out quantitative first to the sample to be checked Dosing unit；

Wherein, first dosing unit includes the first slit and the first sample chamber that is separated by first slit Room and the second sample chamber；The first sample chamber is communicated with the sample injection unit, and second sample chamber is given up with described Liquid unit communicates；

Wherein, third is additionally provided between the reaction member and the waste unit to report an error unit, the third report There is wrong unit chamber, the chamber to be used to receive the sample to be checked via reaction member.

Preferably, which further includes that first be arranged between waste unit and the second sample chamber reports an error list Member, described first reports an error unit with chamber, and the chamber is for receiving the sample to be checked from the second sample chamber；Wherein, institute First unit that reports an error to be stated with optical detection apparatus to be connected, the optical detection apparatus is used to carry out optical detection to the chamber, To judge the amount of liquid in chamber；Herein it should be noted that " connected " can not be described herein is physically connected to, and only position Set alignment.

Preferably, which further includes the liquid storage unit for storage reaction reagent.

Preferably, which further includes for carrying out the second quantitative dosing unit to the reaction reagent；Its In, second dosing unit includes that the second slit and the first reagent chamber separated by second slit and second are tried Agent chamber；First reagent chamber is communicated with the liquid storage unit, and second reagent chamber is communicated with the waste unit.

Preferably, which further includes that second be arranged between waste unit and the second reagent chamber reports an error list Member, described second reports an error unit with chamber, and the chamber is for receiving the reaction reagent from the second reagent chamber；Wherein, institute Second unit that reports an error to be stated with optical detection apparatus to be connected, the optical detection apparatus is used to carry out optical detection to the chamber, To judge the amount of liquid in chamber；Herein it should be noted that " connected " can not be described herein is physically connected to, and only position Set alignment.

Preferably, which further includes mixed cell, for mixing sample to be checked and reaction reagent；Its In, it is provided with mixing arrangement in the mixed cell, for mixing sample to be checked with reaction reagent；Wherein, described mixed It attaches together and sets selected from least one of setting pillar, Z-shaped microchannel, W types mixer and triangle micro-structure.

The third reports an error unit with chamber, which is used to receive via the mixed from mixed cell of reaction member Close liquid；Wherein, the third unit that reports an error is connected with optical detection apparatus, the optical detection apparatus be used for the chamber into Row optical detection, to judge the amount of liquid in chamber；Herein it should be noted that described herein " connected " can not be physics phase Even, only position alignment.

Preferably, which further includes the ventilation unit being connect with the waste unit, for being micro-fluidic core Piece system provides required external pressure；

Wherein, which further includes the tool interface system being connect with the ventilation unit, and the tool interface system is used for The necessary instrument outside flow control chip system is connected, the air pressure that the necessary instrument provides is provided to described by the ventilation unit Micro-fluidic chip system；

Wherein, the necessary instrument includes for providing the pulsometer of air pressure, gas-guide tube and connecting with the ventilation unit The air-tightness interface connect；The air-tightness interface is tubaeform.

Preferably, filter element is additionally provided between the sample injection unit and first dosing unit to treat sample sheet It is filtered.

The second aspect of the utility model also provides a kind of biochemistry detection micro-fluidic chip system, the micro-fluidic chip system It is made of the upper, middle and lower；

Wherein, media layer damage is biochemistry detection micro-fluidic chip as described above；

Wherein, superstructure and understructure close the middle layer for covering；It is provided in superstructure and sample introduction The sample holes of unit connection.

The utility model can obtain following advantageous effect：

1, the utility model detects sample to be checked using microfluidic chip technology combination biochemical analysis method, can examine simultaneously Multiple biochemical indicators of a sample are surveyed, and high with detection sensitivity, detection limit is low, detects the advantages that repeatable, matches simultaneously Specific necessary instrument is closed, full-automatic chip detection may be implemented, accurate testing result can be quickly obtained without human interference.

2, the utility model uses microfluidic chip technology, treats sample and originally carries out volume quantitative first so that only spy The sample for determining volume reacts, and ensures the accuracy of testing result, simultaneously because chip structure is fixed, so for same This its detection repeatability is strong, ensures the stability and reliability of testing result.

3, the utility model uses microfluidic chip technology, only carries out one-time detection for each detection sample, detects Chip is discarded immediately after completion, it is entirely avoided the detection interference in hospital between different samples, while patient is avoided completely Between cross-infection.

4, the utility model uses microfluidic chip technology, and the waste liquid and extra sample after reaction is completed are all completely close It is enclosed in chip interior, the leakage of waste liquid or sample will not be caused, it is nuisanceless to detection environment, to hospital's testing staff's safety Height will not lead to the generation of nosocomial infection.

Description of the drawings

Fig. 1 is a kind of specific biochemistry detection micro-fluidic chip schematic diagram provided by the utility model.

It is single that Fig. 2 shows that the utility model one kind being preferably provided with filtering between sample injection unit and the first dosing unit The partial schematic diagram of member.

Fig. 3 shows a kind of specific necessary instrument and its connection type with biochemistry detection micro-fluidic chip.

Fig. 4 is the structure and its Operational Mechanisms figure of the first dosing unit of chip provided by the utility model；Wherein, Fig. 4 A Show the structure of the first dosing unit, including first baffle 54, first sample chamber 52, the second sample chamber 53, first is narrow Seam 51, and the micro-valve door that is arranged on the microchannel of 52 side of first sample chamber, wherein the first baffle 54 and described the One slit 51 is opposite but does not connect, and 52 and second sample chamber 53 of the first sample chamber passes through the first baffle 54 and institute The space that the first slit 51 defines is stated to communicate；It is shown in Fig. 4 B before quantitative, the push-pull rod first passed through in necessary instrument 9 comes Close micro-valve door；Fig. 4 C are shown, and sample to be quantified, sample to be quantified are added into first sample chamber 52 by sample entrance port It can be first filled in first sample chamber 52, extra liquid can cross the first slit 51 and enter the second sample chamber 53, and then micro- access by being connected to waste unit 4 flows into downstream configurations unit；As Fig. 4 D are shown through necessary instrument 9 Micro-valve door is opened, the sample after quantifying can be flowed into downstream configurations unit.

Fig. 5 is the structure and its Operational Mechanisms figure of the second dosing unit of chip provided by the utility model.

Fig. 6 is the structural schematic diagram of reagent pouch provided by the utility model and the not Tongfang of release prepackage reaction reagent Formula；Wherein, Fig. 6 A are a kind of schematic diagrames of reagent pouch provided by the utility model；Fig. 6 B show broken by external pressure Split the mode of reagent pouch；Fig. 6 C are shown in such a way that needle pierces disrupting agent pouch.

Fig. 7 is the schematic diagram of a layer structure of the biochemistry detection micro-fluidic chip system of the utility model.

Fig. 8 is a kind of schematic diagram of superstructure provided by the utility model.

Reference sign

1 sample injection unit, 2 liquid storage unit, 3 reaction member

4 waste unit, 5 first dosing unit 6 first reports an error unit

7 ventilation unit, 8 tool interface system, 9 necessary instrument

10 second dosing units 11 second report an error 12 mixed cell of unit

13 filter element, 14 third reports an error 21 reagent pouch of unit

51 first slit, 52 first sample chamber, 53 second sample chamber

54 first baffles 91 push 92 gas-guide tube of pulsometer

93 air-tightness interface, 101 second slit, 102 first reagent chamber

103 second reagent chamber, 104 second baffle, 121 mixing arrangement

211 location hole, 212 seal, 213 crush-zone

214 needling structures

Specific implementation mode

The endpoint of disclosed range and any value are not limited to the accurate range or value herein, these ranges or Value should be understood as comprising the value close to these ranges or value.For numberical range, between the endpoint value of each range, respectively It can be combined with each other between the endpoint value of a range and individual point value, and individually between point value and obtain one or more New numberical range, these numberical ranges should be considered as specific open herein.

As shown in Figure 1 and Figure 4, the utility model first aspect provides a kind of biochemistry detection micro-fluidic chip, this is micro-fluidic Chip includes：For receiving the sample injection unit 1 of sample to be checked, providing the anti-of reacting environment for the sample to be checked and reaction reagent Answer unit 3 and the waste unit 4 for collecting waste liquid；The micro-fluidic chip further includes for determining the sample to be checked First dosing unit 5 of amount；

Wherein, first dosing unit 5 includes slit 51 and the first sample chamber separated by first slit 51 Room 52 and the second sample chamber 53；The first sample chamber 52 is communicated with the sample injection unit 1, second sample chamber 53 It is communicated with the waste unit 4；

Wherein, third is additionally provided between the reaction member 3 and the waste unit 4 to report an error unit 14, described Three report an error unit 14 with chamber, which is used to receive the sample to be checked via reaction member 3.

According to the utility model, the first dosing unit 5 is used to the sample to be checked that participate in reaction quantitatively divide and take, It can ensure to only have the sample to be checked of designated volume to participate in subsequent biochemistry by extra sample transport to be checked at waste unit 4 Reaction, to ensure the accuracy of the utility model chip detection.

Quantitative principle is carried out in conjunction with Fig. 4 to first dosing unit 5 to illustrate：As shown in Fig. 4 A, the The primary structure of a certain amount of unit 5 has a first baffle 54, first sample chamber 52, the second sample chamber 53, the first slit 51, and And micro-valve door is provided on the microchannel of 52 side of first sample chamber.Wherein, the first baffle 54 and first slit 51 is opposite but do not connect, and 52 and second sample chamber 53 of the first sample chamber passes through the first baffle 54 and described first The space that slit 51 defines communicates.First dosing unit 5 carries out quantitative main operational principle to sample, is quantifying it Before, the push-pull rod in necessary instrument 9 is first passed through to close micro-valve door (as shown in Fig. 4 B), then by sample entrance port to Sample to be quantified is added in one sample chamber 52, sample to be quantified can be first filled in first sample chamber 52, extra liquid Cognition crosses the first slit 51 and enters the second sample chamber 53, and then micro- access by being connected to waste unit 4 flows into Downstream configurations unit (Fig. 4 C).Then as shown in fig.4d, micro-valve door is opened by necessary instrument 9, the sample meeting after quantifying It is flowed into downstream configurations unit.

Since what chip structure was all made of injection molding is prepared on a large scale mode, so the baffle of each chip interior 54, first sample chamber 52, the second sample chamber 53, the position all same of the first slit 51, to ensure each chip this The volume of the quantitative chamber of a certain amount of unit 5 is identical, and then ensures body of the different chips to the same sample to be tested after quantitative Product is also identical, repeatability between which improves batch interior repeatability of chip and criticize.Optionally, this is fixed Other shapes can also be used by measuring chamber, such as round, trapezoidal, square or other various irregular shapes, quantitative baffle It may be used other shapes, such as vertical baffle, other are variously-shaped for arc-shaped baffle etc..

According to the utility model, by micro- logical between first dosing unit 5 and sample injection unit 1 and waste unit 4 Road is attached, in order to the flowing of liquid.Preferably, the connector between microchannel and waste unit 4 is located at waste unit 4 top, main function are that the waste liquid after the completion of reacting or extra sample to be checked are transported in waste unit 4, simultaneously Since the structural relation on position so that chip front will not be flowed back by microchannel into the waste liquid inside waste unit 4 Point.

Wherein, the shape of the cross section of the microchannel can be rectangle, round, triangle, trapezoidal or other various shapes Shape.The width of microchannel 215 can be 0.001mm-10mm, and depth can be 0.001mm-10mm.

Wherein, it is preferably provided with piston structure in the micro-valve door, opening or closing can be completely by instrument according to pre- Program is determined to complete.Usually not under the intervention of instrument, micro-valve door is in the open state or part micro-valve door is in the open state, And when needing to be turned off, the piston of micro-valve door is driven by the shaft of stepper motor in instrument, and piston is rotated by a certain angle, So that the fluid path of internal piston is closed so that the transformation of micro-valve door is in off state.It is preferred, therefore, that necessary instrument 9 It further include stepper motor.

As a kind of alternative embodiment, the piston can be vertical slide and non-rotating, in general state The piston of lower micro-valve door is in the open state, and when needing to close, the piston at micro-valve door is pushed by the push-pull rod of instrument, will Piston is pushed into inside microchannels to block microchannel so that the micro-valve door is closed.

As another alternative embodiment, micro-valve door can also be opened by the cooperation of iron plate and electromagnetic field And shutoff operation places electromagnet, general for example, a small iron plate can be pasted above micro-valve door below micro-valve door Under state, electromagnet no power, iron plate is located above micro-valve door, and micro-valve door is in the open state, when needing to close, passes through instrument Device to be powered to electromagnet, and the electromagnetic field that magnet generates can attract the iron plate above micro-valve door, to move iron plate to micro-valve door Lower section so that micro-valve door is closed.

Herein it should be noted that, although being as above only to the micro-valve door and the first quantitative list in the first dosing unit 5 The introduction that member 5 is carried out with waste unit 4 by the connection type of micro- access and position, but it is equal to the micro-valve door referred to below It is applicable in.In the case of no explanation on the contrary, the effect and selection of the micro-valve door hereinafter referred to and other structures unit are logical It crosses mode that micro- access is connect with waste unit 4 and position can refer to above.

According to the utility model, under preferable case, in order to ensure that detection can be smoothed out, the utility model it is micro-fluidic Chip further includes that first be arranged between the first dosing unit 5 and waste unit 4 reports an error unit 6, and described first reports an error unit 6 With chamber structure, which is used to receive the sample to be checked of the second sample chamber 53 from the first dosing unit 5.To be checked Sample is by the first dosing unit 5 and carries out after quantifying, and extra sample to be checked can be entered by extracting under external force First report an error unit 6 chamber structure in, and according to the filling degree of liquid in the chamber structure come judge to detect whether can It is smoothed out.If hydraulically full in chamber structure, it is believed that be also filled at first sample chamber 52 in the first dosing unit 5 Liquid, at this time it is believed that chip operation is normally without reporting an error, if first report an error unit 6 chamber structure inside without liquid or Amount of liquid is inadequate, then it is assumed that the liquid volume of first sample chamber 52 is also insufficient in the first dosing unit 5, needs to report an error simultaneously at this time Terminate the detection of chip.Chip interior self-detection mechanism can ensure chip ability in the case of only liquid quantitative entirely accurate in this way It will continue to carry out subsequent detection, to ensure the accuracy of testing result.

Wherein, it is preferred that described first unit 6 that reports an error is connected with optical detection apparatus, and the optical detection apparatus is available In carrying out optical detection to the chamber, to judge the amount of liquid in chamber.

According to the utility model, the sample to be checked can not directly carry out the biochemical inspection of multi objective by any processing It surveys, can also according to circumstances carry out processing appropriate, for example, dilution etc..In this case, chip provided by the utility model is also It (is made of reagent pouch 21 and microchannel) including liquid storage unit 2, for storing the reagent treated sample and originally handled, example Such as, dilution.

According to the utility model, the liquid storage unit 2 is at least one, and Fig. 1 shows 1 liquid storage unit 2, but number is simultaneously It is not restrictive condition, according to other application scene, the number of liquid storage unit 2 can be increased or decreased.The liquid storage unit 2 can incite somebody to action Sample processing reagent, the various reaction reagents for participating in biochemical reaction etc. are loaded into the chip interior of the utility model in advance, at this When utility model chip carries out the test of sample to be checked, then by external force the reagent inside the liquid storage unit 2 is released in sequence It releases.It, can not also herein it should be noted that the size and shape of each liquid storage unit 2 of chip interior can be identical Together, shape is also not limited to circle shown in figure, other shapes such as rectangle, diamond shape, polygon or irregular shape It is equally applicable.The size of each liquid storage unit 2 can change according to the volume size of the reagent to be pre-installed simultaneously, at this time The volume of liquid storage unit 2 simultaneously differs.

As shown in Figure 6A, the utility model provides a kind of basic structure of liquid storage unit 2, and prepackage reaction reagent is pre- It is first loaded into reagent pouch 21, which is preferably flexible material, which includes but not limited to：Nitrocellulose Film, plastic film or metal aluminum foil, the plastic film can be but be not limited to polyester, polyethylene terephthalate (PET), at least one of makrolon (PC), polypropylene (PP) and polymethyl methacrylate (PMMA).Its common feature It is that can discharge internal prepackage reaction reagent by way of squeezing or needle pierces.It is loaded into reagent pouch in prepackage reaction reagent After in 21, outlet is sealed to form seal 211 by way of thermoplastic envelope.The seal degree of seal 211 herein Than shallower so that when external force pressurizes reagent pouch 21, seal 211 can rupture.Location hole 212 is for assisting reagent capsule Bag 21 is fixed at the liquid storage unit 2 in chip.When needing to discharge 2 internal-response reagent of liquid storage unit, Ke Yitong The mode in Fig. 6 B is crossed, by squeezing the crush-zone 213 in reagent pouch 21, so that seal 211 is ruptured and discharged Liquid can also puncture the bottom section such as needling structure 214 of reagent pouch 21 by pricker by the way of in Fig. 6 C Place so that inside prepackage reaction reagent releases.

According to a kind of preferred embodiment of the utility model, the chip further includes being quantified to the reaction reagent The second dosing unit 10, for the accurate quantitative of reaction reagent.For example, when need to treat sample be originally diluted when, The diluted sample of predetermined extension rate can be obtained.It is as shown in Figure 5, second dosing unit 10 include the second slit 101, And the first reagent chamber 102 and the second reagent chamber 103 separated by second slit 101；First reagent chamber 102 communicate with the liquid storage unit 2, and second reagent chamber 103 is communicated with the waste unit 4.Preferably, described second Dosing unit 10 further includes second baffle 104, and the second baffle 104 is opposite with second slit 101 but does not connect, institute State what the first reagent chamber 102 and the second reagent chamber 103 were defined by the second baffle 104 and second slit 101 Space communicates.Wherein, the quantitative principle of second dosing unit 10 is identical as the quantitative principle of the first dosing unit 5, herein It is no longer repeated.

It is further preferred that in order to further ensure that the reaction reagent that can obtain predetermined amount, which also wraps It includes second be arranged between waste unit 4 and the second reagent chamber 103 to report an error unit 11, described second unit 11 that reports an error has Chamber, the chamber are used to receive the liquid of the second reagent chamber 103 from the second dosing unit 10.Wherein, second report The principle that reports an error of wrong unit 11 reports an error with first, and the principle that reports an error of unit 6 is identical, and it is no longer repeated herein.

Wherein, more preferably, described second unit 11 that reports an error is connected with optical detection apparatus, the optical detection apparatus For carrying out optical detection to the chamber, to judge the amount of liquid in chamber.

It is herein it should be noted that, although as shown in Figure 1, merely define a liquid storage unit 2 and same Second dosing unit 10.But those skilled in the art it should be appreciated that, the second dosing unit 10 can by quantity with And the setting of connection type, different reaction reagents is quantified.These should be considered as the protection model in the utility model Within enclosing.

According to the utility model, before biochemical reaction, need to treat the feelings that sample is originally anticipated (for example, dilution) Under condition, the micro-fluidic chip further includes mixed cell 12, for mixing sample to be checked and reaction reagent, and may be sent out Raw pre-reaction.

In order to enable sample to be checked and reaction reagent are sufficiently mixed, in the case of preferred, it is arranged in the mixed cell 12 It is that assisted reaction reagent and sample to be checked are mixed to have mixing arrangement 121, main function.Embodiment party as one preferred Formula, the mixing arrangement 121 are made of multiple setting pillars, which can be located at 12 inside both sides of mixed cell, When being extruded inside mixed cell 12, internal liquid can be promoted to be mixed, while liquid, when by erectting pillar, meeting exists Small vortex is formed around pillar, the disturbance of the small vortex can accelerate the mixing between liquid, to accelerate between each component Reaction (if necessary to react).Although mixing arrangement 121 shown here is realized in the form of erectting pillar, conduct Other alternative micro-structures, such as Z-type microchannel, W type mixers, the other structures such as triangle micro-structure form can also assist simultaneously Accelerate to mix between reagent.

According to the utility model, can be entered in reaction member 3 after sample to be checked is optionally combined with reaction reagent Carry out the biochemical reaction of multi objective.In order to obtain accurately as a result, according to a kind of preferred embodiment of the utility model, in institute It states and is additionally provided with third between reaction member 3 and the waste unit 4 and reports an error unit 14, the third unit 14 that reports an error has chamber Room, the chamber are used to receive the mixed liquor via reaction member 3 from mixed cell 12 (when need not treat sample this progress When pretreatment, it is sample to be checked by reaction member 3 that the third, which reports an error that unit 14 receives).Wherein, the third reports an error list The principle that reports an error of member 14 reports an error with first, and the principle that reports an error of unit 6 is identical, and it is no longer repeated herein.

Preferably, the third unit 14 that reports an error is connected with optical detection apparatus, and the optical detection apparatus is used for institute It states chamber and carries out optical detection, to judge the amount of liquid in chamber.

Reaction member 3 provided by the utility model is the place that biochemical reaction occurs and carries out optical detection, although in Fig. 1 8 reaction members are illustrated only, correspond to 8 Testing index, but specific number is not restrictive condition, according to different applications Scene can increase or decrease the number of the reaction member 3 to complete the Indexs measure of more or less numbers.In each reaction In unit, reagent needed for each Testing index is preferably stored in 3 inside of reaction member in the form of freeze dried powder (can also be pre- First it is packaged in liquid storage unit 2, when needed, in release to reaction member 3), when needing detection, upstream micro-valve door is opened, The extracting effect for coordinating necessary instrument 9, in sample injection unit 1 (need to treat sample originally pre-processed when, be mixed cell 12) internal sample to be checked (or pretreatment fluid in mixed cell 12) will be flowed into each reaction member, last preferred Third can be entered to report an error in unit 14, extra sample to be checked (or pretreatment fluid in mixed cell 12) can be drawn into useless In liquid unit 4.Sample to be checked (or pretreatment fluid in mixed cell 12) into each reaction member can dissolve internal prestore Powdered reagent, and biochemical reaction occurs, generates certain colour developing as a result, by the optical detecting module of necessary instrument 9, can be right Each reaction member carries out optical detection, to obtain the testing result of each index.

According to the utility model, the waste unit 4 is preferably placed at the end of liquid flow path system in chip, to collect The waste liquid of each step biochemical reaction prevents waste liquid outflow chip and pollutes external environment.Wherein, the waste unit 4 in structure simultaneously It is not limited to rectangle shown in Fig. 1, other shapes, such as cylinder, it is conical or other various irregular shapes also same Sample is applicable in.Wherein, the volume of the waste unit 4 should be greater than all liquid storage units 2 (in the presence of) interior reagent The sum of the volume of volume and sample to be checked, to can guarantee waste unit 4 can to issuable waste liquid in reaction process into Row is effective to be collected.

According to the utility model, the sample injection unit 1 is used to receive the sample to be checked of user's injection.The sample to be checked can Think the whole blood of human or animal, blood plasma, urine, saliva, sperm, spinal cord is one or more in the body fluid such as amniotic fluid.In addition, this Utility model chip can be also used for field of detection of food safety, and detection sample can be food leachate, extracting solution, elution Liquid or other liquid, to remains of pesticide in food, the bacterium in food, virus or other pathogens, nutrition contained by food product The content of substance is detected.Likewise, the utility model chip can also be applied to environmental testing, sample is detected at this time Can be river water, seawater, or any liquid for being derived from environment to be detected, it can be organic to the poisonous and harmful substance in environment Or inorganic pollution is used for quickly detecting.

Wherein, the structure of the sample injection unit 1 can be cylindrical, conical (for example, structure shown in Fig. 1), ladder Shape or other various irregular shapes.Its major function is to receive the sample to be checked of user's injection so that sample to be checked is being noted The leakage of sample to be tested will not occur after entering to chip, sample can only be operated according to specific microchannel structure.

As an alternative embodiment, the sample injection unit 1 can be made into capillarity sampling structure, note at this time The sample to be checked entered can wick themselves into the structural unit in downstream, and sample to be checked can be adsorbed on by capillarity In chip, prevents the leakage of sample to be tested and pollute environment.

According to the utility model, when the sample to be checked is sample to be checked (for example, the whole blood sample) for needing to filter, institute It further includes the filtering list being arranged between the sample injection unit 1 and first dosing unit 5 to state biochemistry detection micro-fluidic chip First 13 (as shown in Figure 2), that is, the filter element 13 is located at the downstream of sample injection unit 1, the upstream of the first dosing unit 5. The filter element 13 is used to filter granule foreign or erythrocyte (for example, when sample to be checked is whole blood) in sample to be checked Deng, while the filter element 13 is also prevented from after measuring samples are loaded and enters in advance in the first dosing unit 5, influences final Reaction result.

According to a kind of preferred embodiment of the utility model, the utility model passes through between micro- access to chip system Pressure or decompression are carried out to control the flowing of each liquid, to enter another structural unit from a structural unit.Cause This, the micro-fluidic chip further includes the ventilation unit 7 being connect with the waste unit 4, for being carried for micro-fluidic chip system For required external pressure, so that internal liquid runs well under the auxiliary of external pressure.Ventilation unit 7 can also be prevented simultaneously Only the waste liquid in waste unit 4 flows out to chip exterior and pollutes external environment.Although ventilation unit 7 as shown in Figure 1 uses W Type microchannel is designed, but other are designed, such as round, arc, other various structures such as Z-type, which can similarly play, ventilates and prevent Only the effect of waste liquid outflow, these designs should all also be treated as being within the protection scope of the utility model.

Preferably, predetermined substance can also be filled inside the ventilation unit 7, the work for playing ventilation but preventing waste liquid from outflowing With, for example the substance can be aerosol, or ventilative but fluid-tight loose cavernous structure substance.

According to a kind of preferred embodiment of the utility model, the micro-fluidic chip of the utility model preferably passes through outside Air pressure needed for 9 offer system of necessary instrument.It is preferred, therefore, that the micro-fluidic chip further includes being connect with the ventilation unit 7 Tool interface system 8, the tool interface system 8 is used to connect necessary instrument 9 outside flow control chip system, and the necessary instrument 9 provides Air pressure the micro-fluidic chip system is provided to by the ventilation unit 7.Wherein, the size of the external pressure can compare Atmospheric pressure is big, can also be smaller than atmospheric pressure, and the size of the air pressure can be easily adjusted according to specific service condition difference.Together When, the tool interface system 8 is preferably air-tightness, i.e. chip, should when the air pressure regulator with necessary instrument 9 is docked Position is air tight.The function can be realized by plastic sealing ring or other assemblies.The air pressure regulator of necessary instrument 9 can To be air driven pump, gas storage vesica, pulsometer, extrusion pump etc..

According to a kind of preferred embodiment of the utility model, as shown in Figure 3, the necessary instrument 9 includes for carrying For promotion pulsometer 91, gas-guide tube 92 and the air-tightness interface 93 being connect with the ventilation unit 7 of air pressure.When chip to be measured After being put into necessary instrument 9, the stepper motor (not shown) of instrument internal can push pulsometer 91 so that air-tightness Interface 93 is clung at the tool interface system 8 of chip to be measured, and it is air-tightness that this, which is close to mode, can be by being connect in the air-tightness Sealing ring or O-ring are set on mouth 93 to complete.Preferably, the air-tightness interface 93 is tubaeform, in the preferred situation Under, air-tightness interface 93 and tool interface system 8 need not be precisely aligned, it is only necessary to which tool interface system 8 is located at the air-tightness interface 93 Inside.It waits after the completion of being close to, the stepper motor being connect with pulsometer 91 is in the lock state, at this time the position of pulsometer 91 It is fixed.Rotation by another the stepper motor (not shown) being connected with 91 internal piston of pulsometer, can push Or extracting piston, to adjust the air pressure inside pulsometer 91, and then manipulate the air pressure of chip interior.It is needing to chip interior When pressurization, is completed by stepper motor to push piston, on the contrary, when needing to depressurize to chip interior, pass through stepper motor It is realized to extract piston.Chip interior fluid path network can drive fluid to shift due to increase or the reduction of air pressure. After the entire testing process of chip to be measured is completed, by the rotation for the stepper motor being connect with pulsometer 91 come so that this is airtight Property interface be detached from chip.

As shown in Figure 7, second aspect according to the present utility model provides a kind of biochemistry detection micro-fluidic chip system, The micro-fluidic chip system is made of the upper, middle and lower；

Wherein, superstructure and understructure close the middle layer for covering；It is provided in superstructure and sample introduction Sample holes that unit 1 connects, and preferred, and the relief hole corresponding to liquid storage unit 2, for providing external impetus to discharge Reaction reagent in liquid storage unit 2.

As shown in Figure 8, it is provided with sample holes (aperture) above superstructure, is used for the sample-adding of sample to be checked.It is described into Sample hole is communicated with the sample injection unit 1 in media layer damage, to ensure that sample to be checked can enter the sample introduction list by sample holes In member 1.In addition, being further preferably provided at least one relief hole (macropore) in superstructure, the relief hole is tied corresponding to middle level The liquid storage unit 2 of structure, the entrance of the push rod for necessary instrument 9, to provide external pressure for liquid storage unit 2, by liquid storage The reagent of the advance enclosed storage in 2 inside of unit releases, and therefore, the necessary instrument 9 of the utility model further includes at least one Push-pull rod.The relief hole size and shape by the structure size of the liquid storage pouch 21 of liquid storage unit in media layer damage and shape Lai It determining, it is preferred that the diameter of the relief hole can be 0.5mm-50mm, and shape can be round, rectangle, polygon, Diamond shape or even irregular shape etc. are variously-shaped.

Preferably, the material of the upper, middle and lower is each independently selected from such as dimethyl silicone polymer (PDMS), gathers Methyl methacrylate (PMMA), makrolon (PC), polypropylene (PP), polyethylene terephthalate (PET), plastics are thin Film, elastic emulsion, natural rubber, plastics and silica gel.

Preferably, the thickness of superstructure is 0.5-20mm, more preferably 0.5-10mm, further preferably 0.5mm- 5mm；The thickness of media layer damage is 0.5-50mm；More preferably 2mm-20mm, further preferably 3mm-15mm；Understructure Thickness is 0.5-20mm, more preferably 0.5-10mm, further preferably 0.5mm-5mm.

A kind of preparation method of preferred biochemistry detection micro-fluidic chip provided by the utility model preferably includes to walk as follows Suddenly (by taking the chip including liquid storage unit 2 as an example)：

Reaction reagent needed for biochemical reaction is preloaded onto in the reagent pouch 21 by step 1), and by way of thermoplastic envelope The seal 212 of the reagent pouch 21 is sealed；And chip system media layer damage is placed on by location hole 211 At specific liquid storage structure, the reagent pouch 21 of each liquid storage is further fixed on chip system middle level knot by way of thermoplastic envelope On structure.

Step 2) places different lyophilized solids in the media layer damage of the chip inside each differential responses unit 3 The weight of pulvis, the solid powder placed in each reaction member 3 is certain.

Step 3) fits to understructure below media layer damage as above, and laminating type includes but are not limited to ultrasound The modes such as hot melt, gluing, ultraviolet light solidification, thermoplastic envelope.

Superstructure is fitted to the upper surface of media layer damage as above by step 4), and laminating type includes but are not limited to ultrasound The modes such as hot melt, gluing, ultraviolet light solidification, thermoplastic envelope.

Using a kind of stream of preferred biochemistry detection micro-fluidic chip system progress biochemistry detection provided by the utility model Journey is as follows：

Step 1) draws a certain amount of sample to be checked by pipettor, and sample to be checked is added to well, then by miniflow Control chip system, which is placed in necessary instrument 9, to be started to test.

For step 2) necessary instrument 9 by internal stepper motor by pulsometer 91, air-tightness interface 93 is moved to tool interface system 8 It is fixed behind place.The first micro-valve door to report an error between unit 5 and mixed cell 12 is closed by the push-pull rod of necessary instrument 9, then The piston in pulsometer 91 is stripped by stepper motor, under the action of draft, sample to be checked enters sample injection unit 1 In, it then enters back into filter element 13 and is filtered, filtered sample to be checked is sucked into the first dosing unit 5, extra Sample to be checked report an error in unit 6 into first, reported an error unit 6 by the optical detecting unit of necessary instrument 9 to detect first Optical signal (necessary instrument 9 further includes optical detecting unit), if the first chamber for reporting an error unit 6 is that empty or liquid is not full of, Then stop chip detection, and report an error, if liquid is full of herein, continues subsequent detecting step.

Step 3) is likewise, the push-pull rod by necessary instrument 9 is closed between the second dosing unit 10 and mixed cell 12 Micro-valve door and any other than flowing to waste unit 4 of reaction reagent for closing in the second dosing unit 10 flow to it Micro-valve door on the access of his structural unit or access, is then discharged by the push-pull rod of necessary instrument 9 in liquid storage unit 2 successively Each reaction reagent, and enter the second dosing unit 10, while extra reaction reagent can enter second and report an error unit 11, By the optical detecting module of necessary instrument 9 second is measured to report an error amount of liquid in unit 11, do not report an error if hydraulically full into Row operates in next step, and otherwise instrument, which reports an error, terminates chip detection.

Step 4) closes the micro-valve door between mixed cell 12 and reaction member 3, opens the first dosing unit 5 and mixes list The micro-valve door between micro-valve door and the second dosing unit 10 and mixed cell 12 between member 12, in order to which sample and reaction try Agent enters in mixed cell 12, promotes sample and reaction reagent to be sufficiently mixed by the movement of push-pull rod in instrument.

After the completion of step 5) mixing, the micro-valve door between mixed cell 12 and reaction member 3 is opened, it is mixed in mixed cell Close that liquid can be flowed into reaction member 3 and third reports an error in unit 14, extra mixed liquor is by the piston pump extracting in instrument to useless In liquid unit 4,3 in reaction member, mixed liquor can dissolve the solid powder of prepackage, and lysate can give birth to sample to be checked Change reaction.

Step 6) necessary instrument by internal light detection module detects the reaction member 3 respectively and third reports an error unit 14, and testing result is analyzed, it reports an error if third and indicates detection bio-occlusion testing result if unit 14 fills up liquid, it is no This testing result is then abandoned, report detection is abnormal.

The sample volume of biochemistry detection micro-fluidic chip system detection provided by the utility model is 10-200 μ L.

In the utility model, necessary instrument 9 is small portable apparatus, and necessary instrument 9 is in addition to including pulsometer 91, air guide Further preferably include push-and-pull bar unit, stepper motor module, light detection module etc. except pipe 92 and air-tightness interface 93.

The third aspect of the utility model additionally provides biochemistry detection micro-fluidic chip as described above and/or the life Change application of the detection micro-fluidic chip system in biochemistry detection.It is further preferred that the necessary instrument 9 further includes temperature control mould Block, to carry out control on demand to the temperature in chip.

The utility model will be described in detail by embodiment below.

Embodiment 1

The present embodiment is used to illustrate described in the preparation and use using chip provided by the utility model and chip system Chip system carries out multiple determination to whole blood

The present embodiment whole blood Biochemical Indexes to be detected are respectively：Alanine aminotransferase (ALT), aspartic acid ammonia Based transferase (AST), γ-paddy ammonia phthalidyl transferase (γ-GT), alkaline phosphatase (ALP), total protein (TP), albumin (Alb), total cholesterol (TC), triglycerides (TG).

Institute's various detection reagents to be used and sample diluting liquid are purchased from Beijing Li Deman biochemistry in the present embodiment Reagent Company.Processing mode to each detection reagent is that each liquid reagent is obtained respective solid after frozen dried Pulvis.

(1) assembling of chip system

Above-mentioned 300 μ L diluent reagents are mounted in reagent in advance to be mounted in reagent pouch 21 in advance, and by way of thermoplastic envelope The seal 212 of the reagent pouch 21 is sealed by (heating time 30s)；And chip body is placed on by location hole 211 It is that the reagent pouch 21 of each liquid storage is further fixed on core by way of thermoplastic envelope at the specific liquid storage structure of media layer damage On piece system media layer damage.

Understructure is fitted on media layer damage by the cured mode of light-sensitive emulsion (ultraviolet light hardening time 10min), Superstructure is fitted on media layer damage by same light-sensitive emulsion curing mode (ultraviolet light hardening time 10min), so Prepare complete available chip system.

(2) pattern detection

Step 1) draws the whole blood sample to be measured of 50 μ L by pipettor, sample to be checked is added to well, then will be micro- Fluidic chip system, which is placed in necessary instrument 9, to be started to test.

For step 2) necessary instrument 9 by internal stepper motor by pulsometer 91, air-tightness interface 93 is moved to tool interface system 8 It is fixed behind place.The first micro-valve door to report an error between unit 5 and mixed cell 12 is closed by the push-pull rod of necessary instrument 9, then The piston in pulsometer 91 is stripped by stepper motor, under the action of draft, whole blood sample enters sample injection unit 1 In, it then enters back into filter element 13 and is filtered, filtered whole blood sample is sucked into the first dosing unit 5, extra Whole blood sample report an error in unit 6 into first, reported an error unit 6 by the optical detecting unit of necessary instrument 9 to detect first Optical signal stops chip detection, and report an error if the first chamber for reporting an error unit 6 is that empty or liquid is not full of, If liquid is full of herein, continue subsequent detecting step.

Step 3) is likewise, the push-pull rod by necessary instrument 9 is closed between the second dosing unit 10 and mixed cell 12 Micro-valve door and any other than flowing to waste unit 4 of reaction reagent for closing in the second dosing unit 10 flow to it Micro-valve door on the access of his structural unit or access, is then discharged by the push-pull rod of necessary instrument 9 in liquid storage unit 2 successively Dilution, and the second dosing unit 10 is entered, while extra dilution can enter second and report an error unit 11, by matching The optical detecting module of set instrument 9, which measures second and reports an error, dilutes liquid measure in unit 11, and not reporting an error if hydraulically full carries out down Single stepping, otherwise instrument, which reports an error, terminates chip detection.

Step 4) closes the micro-valve door between mixed cell 12 and reaction member 3, opens the first dosing unit 5 and mixes list The micro-valve door between more than 10 mixed cell 12 of micro-valve door and the second dosing unit between member 12, in order to whole blood sample and dilute It releases liquid to enter in mixed cell 12, promotes whole blood sample and dilution to be sufficiently mixed by the movement of push-pull rod in instrument.

After the completion of step 5) mixing, the micro-valve door between mixed cell 12 and reaction member 3 is opened, in mixed cell 12 In mixed liquor can be flowed into reaction member 3 and third reports an error unit 14, extra mixed liquor is arrived by the piston pump extracting in instrument In waste unit 4,3 in reaction member, mixed liquor can dissolve the solid powder of prepackage, and lysate can occur with sample to be checked Biochemical reaction.

Testing result shows：Testing result for each biochemical indicator in whole blood is respectively：Alanine aminotransferase (ALT)：32IU/L, aspartate aminotransferase (AST)：28I/L, γ-paddy ammonia phthalidyl transferase (γ-GT)：25IU/L, alkali Acid phosphatase (ALP)：98IU/L, total protein (TP)：73.25g/L, albumin (Alb)：46.3g/L, total cholesterol (TC)： 5.7mmol/L, triglycerides (TG):1.3mmol/L.The testing result and the testing result of standard biochemical analyzer are carried out It compares, it is found that the correlation between numerical value is high, show that this micro-fluidic chip effect is preferable.

Preferred embodiments of the present invention described in detail above, still, the utility model is not limited to this.At this In the range of the technology design of utility model, a variety of simple variants can be carried out to the technical solution of the utility model, including each Technical characteristic is combined with any other suitable method, these simple variants and combination equally should be considered as the utility model Disclosure of that belongs to the scope of protection of the utility model.

Claims

1. a kind of biochemistry detection micro-fluidic chip, which is characterized in that the micro-fluidic chip includes：For receive sample to be checked into Sample unit (1) provides the reaction member (3) of reacting environment and for collecting waste liquid for the sample to be checked and reaction reagent Waste unit (4)；The micro-fluidic chip further includes for carrying out quantitative the first dosing unit (5) to the sample to be checked；

Wherein, first dosing unit (5) include the first slit (51) and separated by first slit (51) first Sample chamber (52) and the second sample chamber (53)；The first sample chamber (52) communicates with the sample injection unit (1), described Second sample chamber (53) is communicated with the waste unit (4)；

Wherein, third is additionally provided between the reaction member (3) and the waste unit (4) to report an error unit (14), it is described Third reports an error unit (14) with chamber, which is used to receive the sample to be checked via reaction member (3).

2. biochemistry detection micro-fluidic chip according to claim 1, which is characterized in that the micro-fluidic chip further includes useless First be arranged between liquid unit (4) and the second sample chamber (53) reports an error unit (6), and described first unit (6) that reports an error has Chamber, the chamber are used to receive the sample to be checked from the second sample chamber (53)；

Wherein, described first unit (6) that reports an error is connected with optical detection apparatus, and the optical detection apparatus is used for the chamber Optical detection is carried out, to judge the amount of liquid in chamber.

3. biochemistry detection micro-fluidic chip according to claim 1 or 2, which is characterized in that the micro-fluidic chip further includes Liquid storage unit (2) for storage reaction reagent.

4. biochemistry detection micro-fluidic chip according to claim 3, which is characterized in that the micro-fluidic chip further includes being used for Quantitative the second dosing unit (10) is carried out to the reaction reagent；

Wherein, second dosing unit (10) includes the second slit (101) and is separated by second slit (101) First reagent chamber (102) and the second reagent chamber (103)；First reagent chamber (102) and the liquid storage unit (2) phase Logical, second reagent chamber (103) communicates with the waste unit (4).

5. biochemistry detection micro-fluidic chip according to claim 4, which is characterized in that the micro-fluidic chip further includes useless Second be arranged between liquid unit (4) and the second reagent chamber (103) reports an error unit (11), and described second reports an error unit (11) tool There is chamber, which is used to receive the reaction reagent from the second reagent chamber (103)；

Wherein, described second unit (11) that reports an error is connected with optical detection apparatus, and the optical detection apparatus is used for the chamber Room carries out optical detection, to judge the amount of liquid in chamber.

6. biochemistry detection micro-fluidic chip according to claim 3, which is characterized in that the micro-fluidic chip further includes mixing Unit (12), for mixing sample to be checked and reaction reagent；

Wherein, it is provided with mixing arrangement (121) in the mixed cell (12), for mixing sample to be checked with reaction reagent It closes；The mixing arrangement (121) selected from erect in pillar, Z-shaped microchannel, W types mixer and triangle micro-structure at least one Kind.

7. biochemistry detection micro-fluidic chip according to claim 1, which is characterized in that the third report an error unit (14) tool There is chamber, which is used to receive the mixed liquor from mixed cell (12) via reaction member (3)；

Wherein, the third unit (14) that reports an error is connected with optical detection apparatus, and the optical detection apparatus is used for the chamber Room carries out optical detection, to judge the amount of liquid in chamber.

8. biochemistry detection micro-fluidic chip according to claim 1 or 2, which is characterized in that the micro-fluidic chip further includes The ventilation unit (7) being connect with the waste unit (4), for providing required external pressure for micro-fluidic chip system；

Wherein, which further includes the tool interface system (8) being connect with the ventilation unit (7), the tool interface system (8) For connecting the necessary instrument (9) outside flow control chip system, the air pressure that the necessary instrument (9) provides passes through the ventilation unit (7) it is provided to the micro-fluidic chip system；

Wherein, the necessary instrument (9) include for provide the pulsometer of air pressure (91), gas-guide tube (92) and with the ventilation The air-tightness interface (93) of unit (7) connection；The air-tightness interface (93) is tubaeform.

9. biochemistry detection micro-fluidic chip according to claim 1, which is characterized in that the sample injection unit (1) and described Filter element (13) is additionally provided between first dosing unit (5) to be originally filtered to treat sample.

10. a kind of biochemistry detection micro-fluidic chip system, which is characterized in that the micro-fluidic chip system by upper layer, middle level and under Layer composition；

Wherein, media layer damage is the biochemistry detection micro-fluidic chip described in any one of claim 1-9；

Wherein, superstructure and understructure close the middle level for covering；It is provided in superstructure and sample injection unit (1) The sample holes of connection are further preferably provided with and correspond to the relief hole of liquid storage unit (2), for providing external impetus to discharge storage Reaction reagent in liquid unit (2).