CN110426526A - A kind of multi-layer micro-fluidic chips for detection of heavy metal ion - Google Patents
A kind of multi-layer micro-fluidic chips for detection of heavy metal ion Download PDFInfo
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- CN110426526A CN110426526A CN201910731301.4A CN201910731301A CN110426526A CN 110426526 A CN110426526 A CN 110426526A CN 201910731301 A CN201910731301 A CN 201910731301A CN 110426526 A CN110426526 A CN 110426526A
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
- B01F33/00—Other mixers; Mixing plants; Combinations of mixers
- B01F33/30—Micromixers
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/00029—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor provided with flat sample substrates, e.g. slides
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/10—Integrating sample preparation and analysis in single entity, e.g. lab-on-a-chip concept
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/00029—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor provided with flat sample substrates, e.g. slides
- G01N2035/00099—Characterised by type of test elements
- G01N2035/00108—Test strips, e.g. paper
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Abstract
The present invention provides a kind of multi-layer micro-fluidic chips for detection of heavy metal ion, have double-layer structure, and first layer is made of proportioning device, indicator bin device, mixing arrangement, detection zone device；The second layer is made of waste liquid device, gasket, it is characterised in that: the proportioning device of first layer make waste water to be measured be divided into five parts, indicator bin device deposit indicator, U-shaped mixing arrangement mix solution effectively, detect zone device and be lower than mixed zone, prevent from flowing backwards；The waste liquid device of the second layer be it is detachable, gasket prevents waste liquid from leaking out.The technology of the present invention feature: the proportioning device of chip meets the quantitative requirement of spectrophotometer, realizes the quantitative proportioning between waste liquid and indicator to be measured, the hybrid channel of first layer setting and anti-backflow device, improves result accuracy.Whole device overcomes the characteristics of existing volume is big, the device is complicated, the convenient and efficient real-time detection that can be used for outdoor environment in conjunction with Five-channel portable spectrophotometer.
Description
Technical field
The present invention relates to optical analysis detecting instrument technical field, more particularly to a kind of for detection of heavy metal ion
Multi-layer micro-fluidic chips.
Background technique
As time goes on, the progress of science and technology, people's living standard are gradually increased, many problems also generate therewith.Society
Problem of environmental pollution is the most serious, and industrial pollution is one of primary pollution source of today's society, is looked forward in industrial pollution with petroleum
Industry petroleum wastewater produced is the most serious.Heavy metal ion has become a factor critically important in contaminated wastewater.
Currently, the unified definition that heavy metal is not still stringent, refer generally to the metal that specific gravity is greater than 5, there are about 45 kinds, as copper,
Lead, zinc, iron, cobalt, nickel, manganese, cadmium, mercury, tungsten, molybdenum, gold, silver etc..Although the heavy metals such as manganese, copper, zinc are required for vital movement
Microelement, but not vital movement institute is required for most of heavy metal such as mercury, lead, cadmium etc., and all heavy metals are more than certain
Concentration is all toxic to human body.Heavy metal in terms of environmental pollution is primarily referred to as the biologies such as mercury, cadmium, lead, chromium and metalloid arsenic
The significant heavy metal element of toxicity.It is directly discharged into river, lake or ocean if heavy metal element is unprocessed, or enters
In soil, it is contaminated these rivers, lake, ocean and soil since they cannot be biodegradable.In food chain
Under biological magnification, their thousands of hundred times of ground enrichments finally enter human body.If the heavy metal of fish or shellfish accumulation is by the mankind
It is eaten or heavy metal is eaten after the absorption of the crops such as paddy, wheat by the mankind, heavy metal will enter human body, make one to generate
Heavy metal poisoning gently then occurs strange illness (minamata disease, Itai-itai diseases etc.), heavy then lead to death.Secondly, heavy metal in human body can and
The strong interaction of the generations such as protein and enzyme, makes them lose activity, and can also accumulate, cause in some organs of the human body
Slow poisoning, poisoning caused by heavy metals exceeding standard are too numerous to enumerate.
Traditional Heavy Metal Pollution analysis detection is tried after generalling use field sampling by laboratory chemical both at home and abroad
The method that agent and instrument are analyzed, the result of analysis detection is to project feasibility studies, contamination control, environmental warning etc.
Positive directive function is played, but traditional detecting instrument has that volume is big, detection time is long, cannot be detected immediately at the scene
The shortcomings that.It and is using colorimetric method mostly in micro-fluidic core currently based on method of the microflow control technique to detection of heavy metal ion
On piece is detected, but colorimetric method haves the shortcomings that detection sensitivity is low, accuracy is low, and in addition paper chip intensity is lower, for
The sample of low surface tension, the hydrophobic region of paper chip not necessarily have enough hydrophobicitys, and sample may leak.
Summary of the invention
The technical problem to be solved in the present invention is to overcome the shortcomings of the prior art, provides a kind of for heavy metal ion
The multi-layer micro-fluidic chips of detection, it is therefore intended that design one kind using organic glass and quartz capillary as material, in conjunction with photoetching skill
Art production chip matched with Five-channel portable spectrophotometer, using spectrophotometry to the heavy metal in waste water from
Son is detected, and solves the problems, such as quantitative proportioning between spectrophotometry waste liquid to be measured and indicator.So as to improve heavy metal ion
Accuracy, the portability of detection realize outdoor instant detection.
In order to solve the above-mentioned technical problem, the invention adopts the following technical scheme:
A kind of multi-layer micro-fluidic chips for detection of heavy metal ion have double-layer structure, wherein
First layer includes proportioning device, indicator storehouse, mixing arrangement, detection zone, and waste liquid to be measured is entered and led to by injection port
It crosses certain gradient pipeline and enters to the proportioning device, the proportioning device includes multiple quantification areas, each quantification area connection
There are 3 pipelines, be respectively as follows: sample introduction connecting pipe, proportioning device and mixing arrangement connecting pipe and be used for waste liquid and is quantitative rear more
The waste liquid effuser road of waste liquid outflow；Wherein sample introduction connecting pipe is connected with quantification area lower end, waste liquid effuser road and fixed
It measures area top to be connected, waste liquid effuser road and quantification area are with centainly to descending slope to prevent extra waste liquid from flowing backwards；It is fixed
It measures device and is equipped with valve with mixing arrangement connecting pipe, before waste liquid to be measured is entered by injection port, close proportioning device and mix
The valve between setting is attached together, waste liquid to be measured is flowed into after proportioning device by sample introduction connecting pipe since valve hinders, waste liquid to be measured
Proportioning device is filled, extra waste liquid enters waste liquid pool by waste liquid effuser road；Waste liquid to be measured finishes proportioning device filling
Afterwards, indicator is blown by mixing arrangement pipeline by the stomata in indicator storehouse using ear washing bulb, then opens valve, waste liquid to be measured
It is uniformly mixed with indicator in mixing arrangement, finally enters detection zone and detected；
The second layer includes waste liquid pool, and the waste liquid effuser road of the first layer is located above waste liquid pool, the waste liquid pool
Diameter is much smaller than chip diameter, prevents extra waste liquid Interference Detection area；
The first layer, gasket, the second layer successively assemble, and the first layer and the second layer are detachably arranged.
The proportioning device and mixing arrangement connecting pipe are U-shaped mixing tube as a preferred technical solution,.
The pipeline inner surface that multi-layer micro-fluidic chips are included as a preferred technical solution, carries out hydrophobic treatment.
The quantification area and detection zone >=5 as a preferred technical solution,.Detection zone is arranged with circular arrangement or rectangle
Column row, with matching for the portable spectrophotometer of suitable path channels difference arrangement.
Beneficial effect is:
Micro-fluidic chip in the present invention is matched with Five-channel portable spectrophotometer, can detect simultaneously 5 kinds with
On heavy metal ion, have the characteristics that portable, high-throughput；The unique proportioning device of chip can realize waste liquid to be measured and instruction
Quantitative proportioning between agent meets the requirement of spectrophotometric standard measure；U-shaped hybrid channel mixes solution uniformly, each pipeline at
Certain slope declines the refluence for effectively preventing solution, improves the accuracy of micro-fluidic detection；The pipeline of all pipelines of chip
Inner surface all carries out hydrophobic treatment, it is therefore prevented that micro solution attaches phenomenon；The present invention is small in size, has convenient, high-throughput spy
Put the instant detection, it can be achieved that outdoor.
Detailed description of the invention
It, below will be to specific in order to illustrate more clearly of the specific embodiment of the invention or technical solution in the prior art
Embodiment or attached drawing needed to be used in the description of the prior art are briefly described.In all the appended drawings, similar element
Or part is generally identified by similar appended drawing reference.In attached drawing, each element or part might not be drawn according to actual ratio.
Fig. 1 is structural representation of one of the embodiment of the present invention for the multi-layer micro-fluidic chips of detection of heavy metal ion
Figure；
Fig. 2 is that first layer of one of the embodiment of the present invention for the multi-layer micro-fluidic chips of detection of heavy metal ion is bowed
Depending on structural schematic diagram；
Fig. 3 is that the second layer of one of the embodiment of the present invention for the multi-layer micro-fluidic chips of detection of heavy metal ion is bowed
Depending on structural schematic diagram；
Fig. 4 is side view of one of the embodiment of the present invention for the multi-layer micro-fluidic chips of detection of heavy metal ion；
Fig. 5 is proportioning device of one of the embodiment of the present invention for the multi-layer micro-fluidic chips of detection of heavy metal ion
Structural schematic diagram；
Fig. 6 is indicator storehouse of one of the embodiment of the present invention for the multi-layer micro-fluidic chips of detection of heavy metal ion
Structural schematic diagram；
Fig. 7 is valve mechanism of one of the embodiment of the present invention for the multi-layer micro-fluidic chips of detection of heavy metal ion
Schematic diagram；
Fig. 8 is the structure of one of the embodiment of the present invention portable spectrophotometer of Heavy Metals in Waters ion detection
Schematic diagram；
Fig. 9 is the convex lens of one of the embodiment of the present invention portable spectrophotometer of Heavy Metals in Waters ion detection
Mirror partial structural diagram；
Figure 10 is the filter of one of the embodiment of the present invention portable spectrophotometer of Heavy Metals in Waters ion detection
Mating plate partial structural diagram.
Figure 11 is the convex of one of the embodiment of the present invention portable spectrophotometer of Heavy Metals in Waters ion detection
Lens partial structural diagram；
Figure 12 is that structure of one of the embodiment of the present invention for the multi-layer micro-fluidic chips of detection of heavy metal ion is shown
It is intended to；
In figure: 1. ontologies；2. flexible shock reducing support leg；3. power supply unit；4.A/D converter；5. photoelectric conversion device；6. light
Quick resistance；7.MSP430 single-chip microcontroller；8. outside connected switch；9. the fixed bracket in inside；10. micro-fluidic chip card slot；11. display screen；
12 micro-fluidic chip detection zone；13. path channels；14. harness；15.LED lamp；16. convex lens；17. 18. sample presentation of optical filter fills
It sets；19. plane mirror；20. fixed block；21. indicator storehouse；22. detection zone；23. injection port；24. quantification area；25. connecting pipe；
26. waste liquid effuser road；27. valve；28. waste liquid pool；29. stomata；30. mixing arrangement；31. gasket；32. valve control dress
It sets；33. sample outflux；34. valve slot.
Specific embodiment
The technical scheme in the embodiments of the invention will be clearly and completely described below, it is clear that described implementation
Example is only a part of the embodiments of the present invention, instead of all the embodiments.Based on the embodiments of the present invention, this field is general
Logical technical staff every other embodiment obtained without creative efforts belongs to what the present invention protected
Range.
It should be noted that the directional instruction (such as up, down, left, right, before and after ...) of institute in the embodiment of the present invention
It is only used for explaining in relative positional relationship, the motion conditions etc. under a certain particular pose (as shown in the picture) between each component, such as
When the fruit particular pose changes, then directionality instruction also correspondingly changes correspondingly.
In addition, the description for being such as related to " first ", " second " in the present invention is used for description purposes only, and should not be understood as
Its relative importance of indication or suggestion or the quantity for implicitly indicating indicated technical characteristic.Define as a result, " first ",
The feature of " second " can explicitly or implicitly include at least one of the features.In the description of the present invention, " multiple " contain
Justice is at least two, such as two, three etc., unless otherwise specifically defined.
The embodiment of the present invention is a kind of multi-layer micro-fluidic chips for detection of heavy metal ion.The multi-layer micro-fluidic core
Piece is inserted on the protractor specially designed by micro-fluidic chip card slot and carries out detection of heavy metal ion.
A kind of multi-layer micro-fluidic chips for detection of heavy metal ion, please refer to shown in Fig. 1~7, have double-layer structure,
Wherein,
First layer includes proportioning device, indicator storehouse 21, mixing arrangement 30, detection zone 22, and waste liquid to be measured is by injection port 23
It enters into and through certain gradient pipeline and enters to the proportioning device, the proportioning device includes multiple quantification areas 24, each
Quantification area 24 is connected with 3 pipelines, is respectively as follows: sample introduction connecting pipe, proportioning device and mixing arrangement connecting pipe 25 and uses
The waste liquid effuser road 26 of extra waste liquid outflow after waste liquid is quantitative；Wherein sample introduction connecting pipe and 24 lower end phase of quantification area
Even, waste liquid effuser road 26 is connected with 24 top of quantification area, and waste liquid effuser road 26 and quantification area 24 are with centainly to descending
Degree is to prevent extra waste liquid from flowing backwards；Proportioning device and mixing arrangement connecting pipe are equipped with valve 27, and waste liquid to be measured passes through
Before injection port 23 enters, the valve 27 between proportioning device and mixing arrangement is closed, waste liquid to be measured passes through sample introduction connecting pipe stream
Enter since valve hinders after proportioning device, waste liquid to be measured fills proportioning device, extra waste liquid by waste liquid effuser road 26 into
Enter waste liquid pool 28；After waste liquid to be measured fills proportioning device, it will be referred to using ear washing bulb by the stomata 29 in indicator storehouse 21
Show that agent is blown into mixing arrangement pipeline, then opens valve, waste liquid to be measured is uniformly mixed with indicator in mixing arrangement 30, most laggard
Enter detection zone 22 to be detected；
The second layer includes waste liquid pool 28, and the waste liquid effuser road 26 of the first layer is located at 28 top of waste liquid pool, described useless
The diameter of liquid pool 28 is much smaller than chip diameter, prevents extra waste liquid Interference Detection area 22；
The first layer, gasket 31, the second layer successively assemble, and the first layer and the second layer are detachably arranged.
Further, the proportioning device and mixing arrangement connecting pipe are U-shaped mixing tube.
Further, the pipeline outer layer that multi-layer micro-fluidic chips are included is wrapped up by quartz capillary.It prevents micro molten
The capillary phenomenon that liquid generates
Further, the quantification area 24 is 5.Cooperation Five-channel spectrophotometer carry out using.
Detection heavy metal ion Cu is first prepared before experiment2+、Fe2+、Cr6+、Cd2+And Pb2+Etc. corresponding to heavy metal ion
Indicator, quantified.
In the specific implementation, which has double-layer structure to the multi-layer micro-fluidic chips of the embodiment of the present invention, and Fig. 1 is
First layer, Fig. 2 are the second layers.First layer and the second layer are linked together with gasket 31 before instilling waste liquid to be measured, it will
The indicator prepared in advance is added in indicator storehouse 21, closes valve 27.Waste liquid to be measured is added from injection port 23, waste liquid to be measured
Quantification area 24 is flowed into through quartz capillary road, after waste liquid to be measured fills quantification area 24, extra waste liquid passes through waste liquor stream
Pipeline 26 flows into waste liquid pool 28 out, and indicator is blown into mixing arrangement 30 with ear washing bulb by the stomata 29 in indicator storehouse 22, is connect
Opening valve 27, waste liquid to be measured outflow flow into detection zone 22 after mixing in mixing arrangement 30 with indicator and examined
It surveys, the numerical value of the obtained numerical value that finally will test and standard compares the concentration for obtaining each ion.
It please referring to shown in Fig. 7, proportioning device and mixing arrangement connecting pipe 25 open up valve slot 34 for installing valve, and
Valve 27 controls its aperture by valve control device 32, and after finally detecting, sample passes through the sample flow that detection zone 22 opens up
33 outflow of outlet.
The embodiment of the present invention cooperates Five-channel portable spectrophotometer and eight channel portable spectrophotometers to use,
Shape and structure of the reference Fig. 8 into Figure 12, a kind of portable spectrophotometer of Heavy Metals in Waters ion detection,
Including ontology 1,1 bottom of ontology is equipped with flexible shock reducing support leg 2, can be realized the free extension of instrument stabilizer blade, can be according to not
It is adjusted accordingly with environment, so that the detection position of instrument is in horizontality, detection data is more accurate；It presses external
Switch 8, by the light on and off of 7 process control LED light 15 of MSP430 single-chip microcontroller, thus detect the heavy metal in respective optical path channel 13 from
Sub- concentration；LED lamplight passes through 16 optically focused of convex lens, obtains detecting the light of required specific wavelength in light transmission optical filter 17, realizes
Front light splitting；It will test micro-fluidic chip used (multi-layer micro-fluidic chips provided by the embodiment of the present invention) and be stuck in sample conveying device
Detecting instrument is sent on 18 micro-fluidic chip card slot 10；The light of specific wavelength is focused on corresponding micro-fluidic by convex lens 16
Chip detection zone 12, light pass through micro-fluidic chip detection zone 12 and are reflected into photo resistance 6 via plane mirror 19；Photoelectric conversion dress
It sets 5 and converts optical signals to voltage signal, operational amplifier again amplifies signal, and A/D converter 4 converts analog voltage signal
It for digital signal, gives MSP430 single-chip microcontroller 7 and carries out processing calculating, finally will test as the result is shown on display screen 11；Power supply
Device 3 is the power supply of entire instrument；Harness 14 is connected by two fixed blocks 20, can used harness 14 equipment is carried out
Back is carried on the arm, so that equipment uses more safe and convenient；The apparatus structure is simple, convenient to use, and balanced capacity is good, temperature control effect
It is good, long service life.
For the accuracy for guaranteeing detection data, whole device is protected from light processing, excludes external interference.
Detecting instrument uses matched micro-fluidic chip, substitutes traditional cuvette, further realizes convenient, quick mesh
's.
When monochromatic light radiation passes through measured matter solution, by the amount of the material absorbing and the concentration of the substance and the thickness of liquid layer
Degree (optical path length) is directly proportional, relationship such as following formula:
A=-lg (I/I0)=- lgT=KLc
Wherein, A: absorbance；I0: the intensity of incident light；It: the intensity of transmitted light；K: molar absorption coefficient, unit: L
mol-1·cm-1；L: the thickness of light absorbing medium, unit: cm；C: the concentration of extinction material, unit: mol/L.
Substance is to the selective absorbing wavelength of light, and corresponding absorption coefficient is the physical constant of the substance.When known
Its trap can be measured with similarity condition by the test sample wiring solution-forming after the absorption coefficient of certain pure material under certain condition,
The content of the substance in test sample can be calculated by above formula.
About light source, it is seen that the light source needs of light spectrophotometer provide continuous spoke within the scope of the service band of instrument
It penetrates, i.e., light source can emit continuous spectrum, to record a complete absorption spectrum.The embodiment of the present invention selects LED light source,
Its wave-length coverage in 380~780nm and present hand-held, for making the optical instruments such as the spectrophotometer of Quick portable detection more
Light source is done with the white light LEDs of high brightness.There is LED light source cheap, easy acquisition, light source output to stablize compared with conventional light source
Etc. a series of clear advantages.
The light that light source is injected is directional light, and in the portable spectrophotometer that the present invention designs, when light is through filter
The solution that the light that mating plate obtains required wavelength is then passed through detection later needs to get to light into photo resistance, therefore, before this
It needs to converge light for a bit, and convex lens can just make directional light through superrefraction by light-ray condensing, convex lens can root
Select according to focal length length.
Optical filter is a kind of wavelength selector of simple and inexpensive, and effect selectively passes through a wavelength range
Light.The maximum transmission wavelength of optical filter refers to that radiation has maximum transmission rate at the wavelength, and half bandwidth refers to maximum transmission
The wave-length coverage of bands of a spectrum at the half of rate.Half bandwidth is smaller, then monochromatic purity is higher.In spectrophotometer of the present invention
In, optical filter can be used to eliminate veiling glare, to balance different wave length beam intensity in dual-wavelength spectrophotometry.
Plane mirror can be passed through by the filtered light of optical filter, subsequent photo resistance directly generates after receiving illumination
Electric current (be similar to solar battery), unstable due to current signal, present invention employs collection IV conversions, amplification, noise reduction one
The circuit board of body handles low current signal, to guarantee the accuracy of the invention measured to greatest extent.
The embodiment of the present invention selects the two visible light protractor sold in the market and the spectrophotometer of the application to carry out
Experiment.Experiment content are as follows: the reagent of same known each concentration of heavy metal ion is surveyed using three above-mentioned protractors
The different each concentration of heavy metal ion of examination, each instrument test are 20 times to the testing time of same reagent.For the 20 of acquisition
The same concentration of heavy metal ion of a same reagent removes a maximum value, removes a minimum value, and remaining 18 numerical value ask equal
Value, the numerical value of the concentration of heavy metal ion for the reagent measured for the instrument.Many experiments test is to be avoided as much as
Contingency bring error.
Two visible light protractor, is indicated with protractor I and protractor II respectively.
Wherein protractor I is selected as Shanghai instrument electroanalysis 723G, technical indicator:
Wave-length coverage: 320nm~1100nm
Wavelength allowable error: ± 2nm
Wavelength repeatability :≤1nm
Spectral bandwidth: 4nm
Transmittance allowable error: ± 0.5% (T)
Transmittance repeatability :≤0.2% (T)
Transmittance ranges: 0~200.0 (T)
Range of absorbency: -0.301~3.000 (A)
Concentration indication range: 0~9999 (C)
Stray light :≤1% (T) is (at 360nm, with NaNO2Measurement)
Power supply: AC90V~250V.
Protractor II is selected as DR500, technical parameter:
The data of test are as shown in the table.
1. comparative experimental data table of table
As it can be seen from table 1 the embodiment of the present invention, compared with the protractor in the prior art, effect is similar, energy
It is enough accurately to measure each concentration of heavy metal ion.
Presently in connection with Figure of description, the present invention is described further.The above various embodiments is only to illustrate the present invention
Technical solution, rather than its limitations；Although present invention has been described in detail with reference to the aforementioned embodiments, this field
Those of ordinary skill is it is understood that it is still possible to modify the technical solutions described in the foregoing embodiments or right
Some or all of the technical features are equivalently replaced；And these are modified or replaceed, and do not make corresponding technical solution
Essence departs from the scope of the technical solutions of the embodiments of the present invention, and should all cover the model in claim and specification of the invention
In enclosing.
Claims (4)
1. a kind of multi-layer micro-fluidic chips for detection of heavy metal ion, which is characterized in that have double-layer structure, wherein
First layer includes proportioning device, indicator storehouse, mixing arrangement, detection zone, and waste liquid to be measured enters into and through one by injection port
Fixed gradient pipeline enters to the proportioning device, and the proportioning device includes multiple quantification areas, and each quantification area is connected with 3
Pipeline is respectively as follows: sample introduction connecting pipe, proportioning device and mixing arrangement connecting pipe and for the quantitative rear extra waste liquid of waste liquid
The waste liquid effuser road of outflow；Wherein sample introduction connecting pipe is connected with quantification area lower end, and waste liquid effuser road and quantification area are pushed up
End is connected, and waste liquid effuser road and quantification area are with centainly to descending slope to prevent extra waste liquid from flowing backwards；Proportioning device
It is equipped with valve with mixing arrangement connecting pipe, before waste liquid to be measured is entered by injection port, closes proportioning device and mixing arrangement
Between valve, waste liquid to be measured flowed into after proportioning device by sample introduction connecting pipe since valve hinders, and waste liquid to be measured filling is fixed
Device is measured, extra waste liquid enters waste liquid pool by waste liquid effuser road；After waste liquid to be measured fills proportioning device, use
Indicator is blown into mixing arrangement pipeline by the stomata in indicator storehouse by ear washing bulb, then opens valve, waste liquid to be measured and instruction
Agent is uniformly mixed in mixing arrangement, is finally entered detection zone and is detected；
The second layer includes waste liquid pool, and the waste liquid effuser road of the first layer is located above waste liquid pool, the diameter of the waste liquid pool
Much smaller than chip diameter, extra waste liquid Interference Detection area is prevented；
The first layer, gasket, the second layer successively assemble, and the first layer and the second layer are detachably arranged.
2. a kind of multi-layer micro-fluidic chips for detection of heavy metal ion according to claim 1, which is characterized in that institute
It states proportioning device and mixing arrangement connecting pipe is U-shaped mixing tube.
3. a kind of multi-layer micro-fluidic chips for detection of heavy metal ion according to claim 1, which is characterized in that more
The pipeline inner surface that layer micro-fluidic chip is included carries out hydrophobic treatment.
4. a kind of multi-layer micro-fluidic chips for detection of heavy metal ion according to claim 1, which is characterized in that institute
It states quantification area and detection zone is 5-8, detection zone is arranged with circular arrangement or rectangle column row.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116399861A (en) * | 2023-06-07 | 2023-07-07 | 中南大学 | Device and method applied to synchronous detection of heavy metal ions |
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