CN107655844A - Developer, copper ion colorimetric method sensor and its application method and purposes for copper ion colorimetric method - Google Patents
Developer, copper ion colorimetric method sensor and its application method and purposes for copper ion colorimetric method Download PDFInfo
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- CN107655844A CN107655844A CN201711051348.3A CN201711051348A CN107655844A CN 107655844 A CN107655844 A CN 107655844A CN 201711051348 A CN201711051348 A CN 201711051348A CN 107655844 A CN107655844 A CN 107655844A
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- JPVYNHNXODAKFH-UHFFFAOYSA-N Cu2+ Chemical compound [Cu+2] JPVYNHNXODAKFH-UHFFFAOYSA-N 0.000 title claims abstract description 90
- 229910001431 copper ion Inorganic materials 0.000 title claims abstract description 89
- 238000004737 colorimetric analysis Methods 0.000 title claims abstract description 44
- 238000000034 method Methods 0.000 title claims abstract description 21
- 238000006243 chemical reaction Methods 0.000 claims abstract description 55
- 239000002096 quantum dot Substances 0.000 claims abstract description 40
- 239000000243 solution Substances 0.000 claims abstract description 36
- 239000007864 aqueous solution Substances 0.000 claims abstract description 18
- 239000007788 liquid Substances 0.000 claims description 30
- 239000000872 buffer Substances 0.000 claims description 20
- 230000002378 acidificating effect Effects 0.000 claims description 16
- 239000012488 sample solution Substances 0.000 claims description 15
- 238000012360 testing method Methods 0.000 claims description 15
- IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 claims description 12
- 238000002835 absorbance Methods 0.000 claims description 9
- CMZYGFLOKOQMKF-UHFFFAOYSA-N 1-(3,5-dimethylphenyl)-3,5-dimethylbenzene Chemical group CC1=CC(C)=CC(C=2C=C(C)C=C(C)C=2)=C1 CMZYGFLOKOQMKF-UHFFFAOYSA-N 0.000 claims description 8
- 239000000203 mixture Substances 0.000 claims description 7
- 239000002904 solvent Substances 0.000 claims description 7
- 229910004613 CdTe Inorganic materials 0.000 claims description 6
- 239000006193 liquid solution Substances 0.000 claims description 5
- SBIBMFFZSBJNJF-UHFFFAOYSA-N selenium;zinc Chemical class [Se]=[Zn] SBIBMFFZSBJNJF-UHFFFAOYSA-N 0.000 claims description 5
- 238000001514 detection method Methods 0.000 abstract description 9
- 230000035945 sensitivity Effects 0.000 abstract description 4
- 238000004458 analytical method Methods 0.000 abstract description 3
- 229910001385 heavy metal Inorganic materials 0.000 abstract description 2
- 239000007979 citrate buffer Substances 0.000 description 8
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 description 7
- 229910052802 copper Inorganic materials 0.000 description 7
- 239000010949 copper Substances 0.000 description 7
- 239000000523 sample Substances 0.000 description 6
- 150000002500 ions Chemical class 0.000 description 5
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 4
- 235000010290 biphenyl Nutrition 0.000 description 4
- 239000008055 phosphate buffer solution Substances 0.000 description 4
- 241000208140 Acer Species 0.000 description 3
- 238000010521 absorption reaction Methods 0.000 description 3
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 description 3
- 238000010586 diagram Methods 0.000 description 3
- 125000000118 dimethyl group Chemical group [H]C([H])([H])* 0.000 description 3
- 239000011521 glass Substances 0.000 description 3
- IOVCWXUNBOPUCH-UHFFFAOYSA-M Nitrite anion Chemical compound [O-]N=O IOVCWXUNBOPUCH-UHFFFAOYSA-M 0.000 description 2
- 229910021529 ammonia Inorganic materials 0.000 description 2
- 239000007853 buffer solution Substances 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 235000009508 confectionery Nutrition 0.000 description 2
- 239000003651 drinking water Substances 0.000 description 2
- 235000020188 drinking water Nutrition 0.000 description 2
- 238000009472 formulation Methods 0.000 description 2
- VEXZGXHMUGYJMC-UHFFFAOYSA-N hydrochloric acid Substances Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 239000002105 nanoparticle Substances 0.000 description 2
- 229940005654 nitrite ion Drugs 0.000 description 2
- 230000003287 optical effect Effects 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 208000027219 Deficiency disease Diseases 0.000 description 1
- 238000000862 absorption spectrum Methods 0.000 description 1
- 239000003929 acidic solution Substances 0.000 description 1
- 238000001479 atomic absorption spectroscopy Methods 0.000 description 1
- XJHABGPPCLHLLV-UHFFFAOYSA-N benzo[de]isoquinoline-1,3-dione Chemical class C1=CC(C(=O)NC2=O)=C3C2=CC=CC3=C1 XJHABGPPCLHLLV-UHFFFAOYSA-N 0.000 description 1
- 238000006555 catalytic reaction Methods 0.000 description 1
- 238000010531 catalytic reduction reaction Methods 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 239000011248 coating agent Substances 0.000 description 1
- 238000000576 coating method Methods 0.000 description 1
- 239000002131 composite material Substances 0.000 description 1
- TVZPLCNGKSPOJA-UHFFFAOYSA-N copper zinc Chemical compound [Cu].[Zn] TVZPLCNGKSPOJA-UHFFFAOYSA-N 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 230000005518 electrochemistry Effects 0.000 description 1
- 238000009713 electroplating Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000001917 fluorescence detection Methods 0.000 description 1
- 239000007850 fluorescent dye Substances 0.000 description 1
- 238000001215 fluorescent labelling Methods 0.000 description 1
- 229910052737 gold Inorganic materials 0.000 description 1
- 238000009616 inductively coupled plasma Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 230000005012 migration Effects 0.000 description 1
- 238000013508 migration Methods 0.000 description 1
- RECVMTHOQWMYFX-UHFFFAOYSA-N oxygen(1+) dihydride Chemical compound [OH2+] RECVMTHOQWMYFX-UHFFFAOYSA-N 0.000 description 1
- 230000001699 photocatalysis Effects 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 150000003222 pyridines Chemical class 0.000 description 1
- 238000006862 quantum yield reaction Methods 0.000 description 1
- 230000005855 radiation Effects 0.000 description 1
- 230000004044 response Effects 0.000 description 1
- PYWVYCXTNDRMGF-UHFFFAOYSA-N rhodamine B Chemical compound [Cl-].C=12C=CC(=[N+](CC)CC)C=C2OC2=CC(N(CC)CC)=CC=C2C=1C1=CC=CC=C1C(O)=O PYWVYCXTNDRMGF-UHFFFAOYSA-N 0.000 description 1
- 229940043267 rhodamine b Drugs 0.000 description 1
- 239000004065 semiconductor Substances 0.000 description 1
- 239000010865 sewage Substances 0.000 description 1
- 229910052709 silver Inorganic materials 0.000 description 1
- 238000004611 spectroscopical analysis Methods 0.000 description 1
- 230000007480 spreading Effects 0.000 description 1
- 238000003892 spreading Methods 0.000 description 1
- 230000001960 triggered effect Effects 0.000 description 1
- 238000005303 weighing Methods 0.000 description 1
- 229910052724 xenon Inorganic materials 0.000 description 1
- FHNFHKCVQCLJFQ-UHFFFAOYSA-N xenon atom Chemical compound [Xe] FHNFHKCVQCLJFQ-UHFFFAOYSA-N 0.000 description 1
- XLOMVQKBTHCTTD-UHFFFAOYSA-N zinc oxide Inorganic materials [Zn]=O XLOMVQKBTHCTTD-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
- G01N21/31—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
- G01N21/33—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry using ultraviolet light
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
- G01N21/78—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a change of colour
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
- G01N2021/775—Indicator and selective membrane
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- Physics & Mathematics (AREA)
- Chemical & Material Sciences (AREA)
- Biochemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Analytical Chemistry (AREA)
- Spectroscopy & Molecular Physics (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Engineering & Computer Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Plasma & Fusion (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
Abstract
The present invention is used for the developer, copper ion colorimetric method sensor and its application method and purposes of copper ion colorimetric method, belongs to heavy metal analysis technical field.It is cheap its purpose is to provide a kind of cost, simple to operate, the developer, copper ion colorimetric method sensor and its application method and purposes for copper ion colorimetric method without detection device.The developer that the present invention is used for copper ion colorimetric method includes quantum dot solution, and the quantum dot solution is the aqueous solution of quantum dot.Copper ion colorimetric method sensor of the present invention includes housing, reaction vessel and light source；The light source and reaction vessel are in housing；The reaction vessel is located at housing inner bottom part, and the light source is located above reaction vessel；The reaction vessel is provided with multiple reactive tanks；The light source is the light source containing ultraviolet light of the wavelength less than 350nm.Copper ion colorimetric method sensor cost provided by the invention is cheap, simple to operate, and qualitative detection can be carried out without detection device, has the sensitivity of superelevation.
Description
Technical field
The invention belongs to heavy metal analysis technical field, more particularly to a kind of colorimetric sensor for being used to detect copper ion
(colorimetric sensor) and preparation method thereof and purposes.
Background technology
Copper is a kind of element being widely present in nature, and in human body it is micro- necessary to one kind, in human body
Played an important role in metabolic processes.Can cause deficiency disease when human body copper intake deficiency, but excess intake but
It may cause to be poisoned, serious results even in death.Due to the exploitation of copper zinc ore, intermetallic composite coating, electroplating process, machine-building
And the discharge of sanitary sewage and house refuse, make copper pollution on the environment problem getting worse.At present both at home and abroad to ring
The presence level of copper ion has strict requirements in border, as Environmental Protection Agency provides that the concentration of copper in drinking water ion is
1.3mg/L, the maximum migration amount of copper ion is 156mg/kg in European Union's toy new command 2009/48/EC regulation toys, China's life
Also provided in sanitary standard for drinking water GB 5749-2006 living, the limit value of copper is 1mg/L.Therefore, highly sensitive, high selection is developed
Property, simple, quickly measure copper ion method has great importance.
The detection method of copper ion mainly has cold atomic absorption spectrometry, inductively coupled plasma spectrometry method, electrochemistry
Method, fluorescence detection and colorimetric method etc..In these methods, colorimetric method has and can directly visually observed, simple, quick, cost
It is cheap, without advantages such as professional detecting instruments.Traditional colorimetric sensor is mainly based upon chemical probe, such as spreading out for rhodamine B
Biology, triazolyl azo analog derivative, pyridine derivatives, 1,8-naphthalimide derivative etc., pass through copper ion and change
Probe is detected with reference to the color change of rear product.However, stoichiometric relationship is limited by, conventional colorimetric sensor
Sensitivity is typically relatively low.To improve sensitivity, various nano-particles, such as Au, Ag, ZnO nano-particle is also widely used in
The probe of colorimetric determination copper ion.Quantum dot (Quantum dots) has as a kind of emerging nano semiconductor material
The characteristics such as fluorescence quantum yield is high, photochemical stability is good, are a kind of extremely potential fluorescence probes, its fluorescence, light
Electroactive and chemiluminescence etc. is applied to the fields such as fluorescence labeling and chemical analysis.At present, based on quantum dot light catalysis also
There is not been reported for the copper ion colorimetric sensor of originality.
The content of the invention
It is simple to operate the technical problem to be solved in the present invention is to provide a kind of cost is cheap, without being used for for detection device
The developer of copper ion colorimetric method, copper ion colorimetric method sensor and its application method and purposes.
The present invention relates to a kind of developer for copper ion colorimetric method, the developer includes quantum dot solution, described
Quantum dot solution is the aqueous solution of quantum dot.
Preferably, the quantum dot includes CdTe/CdS quantum dots, ZnSe/ZnS quantum dots or Mn/ZnS quantum dots.
Preferably, the developer also include 3,3', 5,5'- tetramethyl biphenyl amine aqueous solutions, acidic buffer, described 3,
The solvent of 3', 5,5'- tetramethyl biphenyl amine aqueous solution is DMSO；The pH value of the acidic buffer is 3-6.
Preferably, the acidic buffer is citrate buffer solution, hac buffer, phosphate buffer solution or sweet ammonia
Acid-hydrochloric acid buffer solution.
Preferably, the concentration of the citrate buffer solution is 0.05-0.2M.
The invention further relates to a kind of copper ion colorimetric method sensor, the sensor includes housing, reaction vessel and light source；
The light source and reaction vessel are in housing；
The reaction vessel is located at housing inner bottom part, and the light source is located above reaction vessel；
The reaction vessel is provided with multiple reactive tanks；
The light source is the light source containing ultraviolet light of the wavelength less than 350nm.
Preferably, camera is additionally provided with above the reaction vessel, for recording the color of reaction solution in reaction vessel.
Preferably, the reaction vessel is white or transparent.
Preferably, the reaction vessel is whiteware cuvette or clear glass colorimetric cylinder.
Preferably, the light source be 254nm ultraviolet lamp tube, power 10W.
The invention further relates to a kind of application method of copper ion colorimetric method sensor, the described method comprises the following steps：
(1) mix：Developer, testing liquid are well mixed and obtain mixed liquor, mixed liquor is placed in the anti-of reaction vessel
Answer in groove, the volume of the mixed liquor is 100~600 μ L；The testing liquid is standard liquid or sample solution；
(2) react：After 3~5min of light source irradiation reaction vessel, stop irradiation and stop reaction；The light source is to contain ripple
The light source of the long ultraviolet light less than 350nm.
(3) record：With the reacted color of cameras record testing liquid；
(6) contrast：The reacted color of sample solution of step (3) record and the reacted color of standard liquid are carried out
Contrast, you can draw the concentration of copper ion in sample solution.
The invention further relates to a kind of application method of copper ion colorimetric method sensor, the described method comprises the following steps：
(1) mix：Developer, testing liquid are well mixed and obtain mixed liquor, mixed liquor is placed in the anti-of reaction vessel
Answer in groove, the volume of the mixed liquor is 100~600 μ L；
(2) react：After 3~5min of light source irradiation reaction vessel, stop irradiation and stop reaction；
(3) detect：With mixed liquor absorbance at 650nm after ultraviolet specrophotometer measure reaction；
(4) calculate：The standard curve of the absorbance making various concentrations copper ion of standard liquid is measured according to step (3),
Then the concentration of copper ion in sample solution is calculated with the absorbance for measuring sample solution according to standard curve；
The testing liquid is standard liquid or sample solution；
The light source is the light source containing ultraviolet light of the wavelength less than 350nm.
Preferably, content of copper ion is 0.1-2.5 μm of ol/L in the mixed liquor.
Preferably, the developer includes quantum dot solution, and the quantum dot solution is the aqueous solution of quantum dot.
Preferably, the quantum dot includes CdTe/CdS quantum dots, ZnSe/ZnS quantum dots or Mn/ZnS quantum dots.
Preferably, the developer also include 3,3', 5,5'- tetramethyl biphenyl amine aqueous solutions, acidic buffer, described 3,
The solvent of 3', 5,5'- tetramethyl biphenyl amine aqueous solution is DMSO；The pH value of the acidic buffer is 3-6；
The concentration of quantum dot is 30-80nM in the mixed liquor；
The concentration of 3,3' in the mixed liquor, 5,5'- tetramethyl benzidine is 50-100mg/L；
The pH value of the mixed liquor is 3-6.
Preferably, the acidic buffer is citrate buffer solution, hac buffer, phosphate buffer solution or sweet ammonia
Acid-hydrochloric acid buffer solution.
Preferably, the concentration of the citrate buffer solution is 0.05-0.2M.
Sensor of the invention operation principle (as shown in Figure 1)：In an acidic solution, meeting after TMB is irradiated with
TMB oxide (TMBox) is oxidized to, solution is changed into blueness from colourless；Under same radiation parameter, quantum dot and can reaches
TMBox is reduced to TMB, in the case of quantum dot dosage is suitable, solution will present colourless again, reach a dynamic and put down
Weighing apparatus；And the presence of copper ion can reduce the reducing power of quantum dot so that a part of TMBox can not be reduced, copper ion concentration
Higher, remaining TMBox is more, and the blueness of solution is deeper, and the concentration of copper ion is can determine whether by observing solution colour.
The invention further relates to a kind of purposes of copper ion colorimetric method sensor, the sensor be used for detect cupric from
Son.
The present invention be used for copper ion colorimetric method developer, copper ion colorimetric method sensor and its application method and purposes with
Prior art difference is：
1st, copper ion colorimetric method sensor cost provided by the invention is cheap, simply, can be determined without detection device
Property detection, have superelevation sensitivity；
2nd, photo catalytic reduction of the Cleaning Principle of colorimetric sensor provided by the invention based on quantum dot, in association area
There is not been reported；
3rd, sun optical drive can be directly utilized, is suitable for open country using ultraviolet as sensor control condition in the present invention
Outer Site Detection.
Brief description of the drawings
Fig. 1 is the sensor mechanism schematic diagram of copper ion colorimetric sensor of the present invention；
Fig. 2 is the structure of the copper ion colorimetric sensor that uviol lamp triggers in embodiment 1；
Fig. 3 is the structure of the copper ion colorimetric sensor that uviol lamp triggers in embodiment 4；
Fig. 4 is the structural representation of reaction vessel in copper ion colorimetric method sensor in embodiment 1；
Fig. 5 is the structural representation of reaction vessel in copper ion colorimetric method sensor in embodiment 2；
Fig. 6 is that the copper ion colorimetric sensor that uviol lamp triggers in embodiment 9 determines various concentrations copper ion standard liquid
Uv absorption spectra；
Fig. 7 is that the copper ion colorimetric sensor that uviol lamp triggers in embodiment 9 determines various concentrations copper ion standard liquid
Color diagram；
Fig. 8 is that the copper ion colorimetric sensor that uviol lamp triggers in embodiment 9 determines various concentrations copper ion standard liquid
Standard curve；
Fig. 9 is that the light-triggered copper ion colorimetric sensor of the sun determines various concentrations copper ion standard liquid in embodiment 10
Color diagram；
Wherein, 1 is housing, and 2 be light source, and 3 be reaction vessel, and 4 be camera, and 5 be reactive tank, and 3a is the reaction of embodiment 1
Container, 3b are the reaction vessel of embodiment 2.
Embodiment
Pass through the developer for copper ion colorimetric method of following examples and checking test to the present invention, copper ion colorimetric
Method sensor and its application method and purposes are further described.
Embodiment 1
As shown in Figure 2, Figure 4 shows, the copper ion colorimetric method sensor of the present embodiment includes housing 1, reaction vessel 3 and light source 2；
Light source 2 and reaction vessel 3 are in housing 1；
Reaction vessel 3 is whiteware cuvette 3a, and reaction vessel 3 is located at the inner bottom part of housing 1, and the light source 2 is positioned at anti-
Answer the top of container 3；
Whiteware cuvette 3a is provided with 6 reactive tanks 5；
Light source 2 be 254nm ultraviolet lamp tube, power 10W.
Embodiment 2
As shown in figure 5, the copper ion colorimetric method sensor and the difference of embodiment 1 of the present embodiment are：This implementation
The reaction vessel 3 of example is clear glass colorimetric cylinder 3b, and clear glass colorimetric cylinder 3b is provided with 6 reactive tanks 5.
Embodiment 3
The copper ion colorimetric method sensor and the difference of embodiment 1 of the present embodiment are：The light source 2 of the present embodiment is
The sun.
Embodiment 4
As shown in figure 3, the copper ion colorimetric method sensor and the difference of embodiment 1 of the present embodiment are：This implementation
Camera 4 is additionally provided with above the reaction vessel of example, for recording the color of reaction solution in reaction vessel 3.
Embodiment 5
The developer of the present embodiment includes quantum dot solution, 3,3', 5,5'- tetramethyl biphenyls amine aqueous solution and acidic buffer
Liquid, wherein quantum dot solution are ZnSe/ZnS quantum dots, and the solvent of 3,3', 5,5'- tetramethyl biphenyl amine aqueous solutions is sub- for dimethyl
Maple, acidic buffer are citrate buffer solution, and the pH value of citrate buffer solution is 4.
Embodiment 6
The developer of the present embodiment includes quantum dot solution, 3,3', 5,5'- tetramethyl biphenyls amine aqueous solution and acidic buffer
Liquid, wherein quantum dot solution are CdTe/CdS quantum dots, and the solvent of 3,3', 5,5'- tetramethyl biphenyl amine aqueous solutions is sub- for dimethyl
Maple, acidic buffer are hac buffer, and the pH value of hac buffer is 5.
Embodiment 7
The developer of the present embodiment includes quantum dot solution, 3,3', 5,5'- tetramethyl biphenyls amine aqueous solution and acidic buffer
Liquid, wherein quantum dot solution are Mn/ZnS quantum dots, and the solvent of 3,3', 5,5'- tetramethyl biphenyl amine aqueous solutions is DMSO,
Acidic buffer is phosphate buffer solution, and the pH value of phosphate buffer solution is 6.
Embodiment 8
The developer of the present embodiment includes quantum dot solution, 3,3', 5,5'- tetramethyl biphenyls amine aqueous solution and acidic buffer
Liquid, wherein quantum dot solution are ZnSe/ZnS quantum dots, and the solvent of 3,3', 5,5'- tetramethyl biphenyl amine aqueous solutions is sub- for dimethyl
Maple, acidic buffer are glycine-HCI cushioning liquid, and the pH value of glycine-HCI cushioning liquid is 3.
Embodiment 9
Using the optical filter of different wave length on xenon source, TMB citric acid solutions are irradiated, found only
The ultraviolet light for having below 350nm wavelength can aoxidize TMB, therefore the present invention is using the light source of the wavelength containing below 350nm
Content of copper ion is measured.Following operate is carried out using the copper ion colorimetric method sensor in embodiment 1.
Enter one the study show that, copper ion colorimetric sensor of the present invention has linear response for copper ion.
Comprise the following steps that：
(1) accurate formulation ion concentration is respectively 2,5,10,20,30,40,50 μM of copper ion standard liquid；
(2) by 20 μ L, 1 μM of CdTe/CdS quantum dots, 20 μ L, 1500mg/L TMB solution, the copper of 20 μ L various concentrations
Ion and a certain amount of citrate buffer solution solution (0.1M, pH4.5) are respectively placed in the different holes of color board, are mixed, overall
Product is 400 μ L.
(3) color board equipped with above-mentioned nitrite ion is placed in irradiation reaction 3min under the uviol lamp of 254nm wavelength, taken out,
Reacted color is recorded with digital camera, as shown in fig. 7, a to f refers respectively to be not added with copper ion, copper ion concentration is
0.1st, 0.25,0.5,1,1.5,2,2.5 μM when corresponding system color.
(4) ultraviolet absorption peak of the solution in 800-300nm wave-length coverages is determined with ultraviolet specrophotometer.Selection
Absworption peak at 650nm is as detection signal, as shown in fig. 6, the size of ultraviolet absorption peak is relevant with ion concentration, a to f difference
Refer to being not added with copper ion, copper ion concentration 0.1,0.25,0.5,1,1.5,2,2.5 μM when corresponding ultraviolet absorption peak.
Linear relationship as shown in figure 8, copper ion in 0.1-2.5uM concentration ranges, linear, linear equation Y
=0.18187X+0.01328 (unit μM), coefficient correlation 0.99516.
Therefore, the application method of the copper ion colorimetric method sensor of the present embodiment is carried out according to the following steps：
(1) mix：Developer, testing liquid are well mixed and obtain mixed liquor, mixed liquor is placed in the anti-of reaction vessel
Answer in groove, the volume of the mixed liquor is 100~600 μ L；
(2) react：After 3~5min of light source irradiation reaction vessel, stop irradiation and stop reaction；
(3) detect：With mixed liquor absorbance at 650nm after ultraviolet specrophotometer measure reaction；
(4) calculate：The standard curve of the absorbance making various concentrations copper ion of standard liquid is measured according to step (3),
Then the concentration of copper ion in sample solution is calculated with the absorbance for measuring sample solution according to standard curve；
The testing liquid is standard liquid or sample solution；
The light source is the uviol lamp of 254nm wavelength.
Embodiment 10
Because the also wavelength containing below 350nm in sunshine, we directly can be used as triggering light using sunshine
Source；Color board containing developer is placed directly within sunny sunlight and irradiated to determine the copper ion of various concentrations.Using reality
Apply the copper ion colorimetric method sensor in example 4 and carry out following operate.
Comprise the following steps that：
(1) accurate formulation ion concentration is respectively 5 μM, 10 μM, 20 μM, 40 μM and 100 μM of copper ion standard liquid；
(2) by 30 μ L, 1 μM of CdTe/CdS quantum dots, 20 μ L, 1500mg/L TMB solution, the copper of 20 μ L various concentrations
Ion and a certain amount of citrate buffer solution solution (0.1M, pH4.5) are respectively placed in the different holes of color board, are mixed, overall
Product is 400 μ L.
(3) color board equipped with above-mentioned nitrite ion is placed in irradiation reaction 20min under sunshine, takes out, use digital camera
Record reacted color, as shown in figure 9, a to f refers respectively to be not added with copper ion, copper ion concentration 0,0.25,0.5,1,
2nd, 5 μM when corresponding system color.
Therefore, by the way that the reacted color of testing sample and the reacted color of copper ion standard liquid are carried out pair
Than, you can judgement substantially is with the naked eye carried out to copper ion concentration in testing sample, realizes that simple and quick measure copper ion contains
The purpose of amount.
Although the foregoing describing the embodiment of the present invention, it will be appreciated by those of skill in the art that these
It is merely illustrative of, protection scope of the present invention is defined by the appended claims.Those skilled in the art is not carrying on the back
On the premise of principle and essence from the present invention, various changes or modifications can be made to these embodiments, but these are changed
Protection scope of the present invention is each fallen within modification.
Claims (10)
- A kind of 1. developer for copper ion colorimetric method, it is characterised in that：The developer includes quantum dot solution, the amount Son point solution is the aqueous solution of quantum dot.
- 2. the developer according to claim 1 for copper ion colorimetric method, it is characterised in that：The quantum dot includes CdTe/CdS quantum dots, ZnSe/ZnS quantum dots or Mn/ZnS quantum dots.
- 3. the developer according to claim 2 for copper ion colorimetric method, it is characterised in that：The developer also includes 3,3', 5,5'- tetramethyl biphenyl amine aqueous solutions, acidic buffer, the solvent of described 3,3', 5,5'- tetramethyl biphenyl amine aqueous solutions are DMSO；The pH value of the acidic buffer is 3-6.
- A kind of 4. copper ion colorimetric method sensor, it is characterised in that：The sensor includes housing, reaction vessel and light source；The light source and reaction vessel are in housing；The reaction vessel is located at housing inner bottom part, and the light source is located above reaction vessel；The reaction vessel is provided with multiple reactive tanks；The light source is the light source containing ultraviolet light of the wavelength less than 350nm.
- 5. copper ion colorimetric method sensor according to claim 4, it is characterised in that：It is additionally provided with above the reaction vessel Camera.
- 6. copper ion colorimetric method sensor according to claim 4, it is characterised in that：The reaction vessel is for white or thoroughly Bright.
- 7. copper ion colorimetric method sensor according to claim 4, it is characterised in that：The light source is the ultraviolet of 254nm Fluorescent tube, power 10W.
- A kind of 8. application method of copper ion colorimetric method sensor, it is characterised in that：It the described method comprises the following steps：(1) mix：Developer, testing liquid are well mixed and obtain mixed liquor, mixed liquor is placed in the reactive tank of reaction vessel In, the volume of the mixed liquor is 100~600 μ L；The testing liquid is standard liquid or sample solution；(2) react：After 3~5min of light source irradiation reaction vessel, stop irradiation and stop reaction；The light source is small containing wavelength In the light source of 350nm ultraviolet light.(3) record：With the reacted color of cameras record testing liquid；(6) contrast：The reacted color of sample solution and the reacted color of standard liquid of step (3) record are carried out pair Than, you can draw the concentration of copper ion in sample solution.
- A kind of 9. application method of copper ion colorimetric method sensor, it is characterised in that：It the described method comprises the following steps：(1) mix：Developer, testing liquid are well mixed and obtain mixed liquor, mixed liquor is placed in the reactive tank of reaction vessel In, the volume of the mixed liquor is 100~600 μ L；(2) react：After 3~5min of light source irradiation reaction vessel, stop irradiation and stop reaction；(3) detect：With mixed liquor absorbance at 650nm after ultraviolet specrophotometer measure reaction；(4) calculate：The standard curve of the absorbance making various concentrations copper ion of standard liquid is measured according to step (3), then The concentration of copper ion in sample solution is calculated according to standard curve and the absorbance for measuring sample solution；The testing liquid is standard liquid or sample solution；The light source is the light source containing ultraviolet light of the wavelength less than 350nm.
- A kind of 10. purposes of copper ion colorimetric method sensor described in claim 4, it is characterised in that：The sensor is used for Detect bivalent cupric ion.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108717049A (en) * | 2018-04-17 | 2018-10-30 | 太原师范学院 | The detection method of micro copper ion concentration |
| CN110907589A (en) * | 2019-11-21 | 2020-03-24 | 福建师范大学福清分校 | Visible Cu detection based on GQDs photocatalysis2+Method (2) |
| CN112924442A (en) * | 2021-01-25 | 2021-06-08 | 中国检验检疫科学研究院 | Copper ion nano colorimetric detection method based on carbon quantum dot photocatalytic activity |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103529022A (en) * | 2012-07-06 | 2014-01-22 | 南京大学 | Method for quick visual detection of copper ion content based on quantum photoelectric effect |
| CN103969258A (en) * | 2014-05-21 | 2014-08-06 | 山西大学 | Test paper and method for rapidly detecting Cd<2+> by virtue of naked eyes |
| CN207779891U (en) * | 2017-10-31 | 2018-08-28 | 中国检验检疫科学研究院 | Copper ion colorimetric method sensor |
-
2017
- 2017-10-31 CN CN201711051348.3A patent/CN107655844A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103529022A (en) * | 2012-07-06 | 2014-01-22 | 南京大学 | Method for quick visual detection of copper ion content based on quantum photoelectric effect |
| CN103969258A (en) * | 2014-05-21 | 2014-08-06 | 山西大学 | Test paper and method for rapidly detecting Cd<2+> by virtue of naked eyes |
| CN207779891U (en) * | 2017-10-31 | 2018-08-28 | 中国检验检疫科学研究院 | Copper ion colorimetric method sensor |
Non-Patent Citations (2)
| Title |
|---|
| 刘声燕;王益林;杨昆;: "近红外发射CdSeTe量子点测定铜离子", 发光学报, no. 02, 15 February 2014 (2014-02-15) * |
| 苏中华;王亚静;赵环;胡娅梅;唐春然;李霞飞;李进;曾云龙;: "以CdS量子点为荧光探针测定痕量铜", 应用化学, no. 07, 10 July 2011 (2011-07-10) * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108717049A (en) * | 2018-04-17 | 2018-10-30 | 太原师范学院 | The detection method of micro copper ion concentration |
| CN110907589A (en) * | 2019-11-21 | 2020-03-24 | 福建师范大学福清分校 | Visible Cu detection based on GQDs photocatalysis2+Method (2) |
| CN110907589B (en) * | 2019-11-21 | 2022-07-15 | 福建师范大学福清分校 | Visible Cu detection based on GQDs photocatalysis2+Method (2) |
| CN112924442A (en) * | 2021-01-25 | 2021-06-08 | 中国检验检疫科学研究院 | Copper ion nano colorimetric detection method based on carbon quantum dot photocatalytic activity |
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