CN207779891U - Copper ion colorimetric method sensor - Google Patents
Copper ion colorimetric method sensor Download PDFInfo
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- CN207779891U CN207779891U CN201721429029.7U CN201721429029U CN207779891U CN 207779891 U CN207779891 U CN 207779891U CN 201721429029 U CN201721429029 U CN 201721429029U CN 207779891 U CN207779891 U CN 207779891U
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- copper ion
- light source
- reaction vessel
- colorimetric method
- method sensor
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Abstract
The utility model copper ion colorimetric method sensor, belongs to heavy metal analysis technical field.It is a kind of of low cost its purpose is to provide, it is easy to operate, it is not necessarily to the copper ion colorimetric method sensor of detection device.The utility model copper ion colorimetric method sensor includes shell, reaction vessel and light source；The light source and reaction vessel are set in shell；The reaction vessel is located at bottom in shell, and the light source is located above reaction vessel；The reaction vessel is equipped with multiple reactive tanks；The light source is the light source of the ultraviolet light less than 350nm containing wavelength.Copper ion colorimetric method sensor provided by the utility model is of low cost, simply, qualitative detection can be carried out without detection device, the sensitivity with superelevation.
Description
Technical field
The utility model heavy metal analysis technical field, more particularly to a kind of colorimetric sensing for detecting copper ion
Device.
Background technology
Copper is trace element necessary to one kind in a kind of element and human body being widely present in nature, in human body
It plays an important role in metabolic processes.Can cause deficiency disease when human body copper intake deficiency, but excess intake but
It may cause to be poisoned, it is serious to result even in death.Due to the exploitation of copper zinc ore, intermetallic composite coating, electroplating process, machine-building
And the discharge of sanitary sewage and house refuse, make copper pollution on the environment problem getting worse.At present both at home and abroad to ring
In border copper ion there are levels strict requirements, as Environmental Protection Agency provide copper in drinking water ion it is a concentration of
1.3mg/L, the maximum the amount of migration of copper ion is 156mg/kg, China's life in European Union's toy new command 2009/48/EC regulation toys
It is also provided in sanitary standard for drinking water GB 5749-2006 living, the limit value of copper is 1mg/L.Therefore, highly sensitive, high selection is developed
Property, method that is simple, quickly measuring copper ion has great importance.
The detection method of copper ion mainly has cold atomic absorption spectrometry, inductively coupled plasma spectrometry method, electrochemistry
Method, fluorescence detection and colorimetric method etc..In these methods, colorimetric method has and can directly visually observe, simple, quick, cost
It is cheap, without advantages such as professional detecting instruments.Traditional colorimetric sensor is mainly based upon chemical probe, such as spreading out for rhodamine B
Biology, triazolyl azo analog derivative, pyridine derivatives, 1,8-naphthalimide derivative etc., pass through copper ion and change
Probe is detected in conjunction with the color change of rear product.However, it is limited by stoichiometric relationship, conventional colorimetric sensor
Sensitivity is generally relatively low.It is also widely used in improve sensitivity, various nano-particles, such as Au, Ag, ZnO nano-particle
The probe of colorimetric determination copper ion.Quantum dot (Quantum dots) has as a kind of emerging nano semiconductor material
The characteristics such as fluorescence quantum yield is high, photochemical stability is good, are a kind of extremely potential fluorescence probe, fluorescence, light
Electroactive and chemiluminescence etc. has been applied to the fields such as fluorescent marker and chemical analysis.Currently, also based on quantum dot light catalysis
There is not been reported for the copper ion colorimetric sensor of originality.
Utility model content
It is easy to operate the technical problem to be solved by the present invention is to provide a kind of of low cost, without detection device
Copper ion colorimetric method sensor.
The utility model is related to a kind of copper ion colorimetric method sensors, it is characterised in that：The sensor includes shell, anti-
Answer container and light source；
The light source and reaction vessel are set in shell；
The reaction vessel is located at bottom in shell, and the light source is located above reaction vessel；
The reaction vessel is equipped with multiple reactive tanks；
The light source is the light source of the ultraviolet light less than 350nm containing wavelength.
Preferably, it is additionally provided with camera above the sensor.
Preferably, the reactor is white or transparent.
Preferably, the reactor is whiteware color board or transparent glass colorimetric cylinder.
Preferably, the light source is the ultraviolet lamp tube of 254nm, power 10W.
Preferably, the light source is the sun.
The utility model copper ion colorimetric method sensor difference from prior art is：
1, copper ion colorimetric method sensor provided by the utility model is of low cost, simply, can be into without detection device
Row qualitative detection, the sensitivity with superelevation；
2, photo catalytic reduction of the testing principle of colorimetric sensor provided by the utility model based on quantum dot, in correlation
There is not been reported in field；
3, using ultraviolet light as sensor control condition in the utility model, sun optical drive can be directly utilized, is suitble to
It is detected in field condition.
Description of the drawings
Fig. 1 is the structure for the copper ion colorimetric sensor that ultraviolet lamp triggers in embodiment 1；
Fig. 2 is the structure for the copper ion colorimetric sensor that ultraviolet lamp triggers in embodiment 4；
Fig. 3 is the structural schematic diagram of reaction vessel in copper ion colorimetric method sensor in embodiment 1；
Fig. 4 is the structural schematic diagram of reaction vessel in copper ion colorimetric method sensor in embodiment 2；
Fig. 5 is the sensor mechanism schematic diagram of the utility model copper ion colorimetric sensor；
Fig. 6 is that the copper ion colorimetric sensor that ultraviolet lamp triggers in embodiment 1 measures various concentration copper ion standard solution
Uv absorption spectra；
Fig. 7 is that the copper ion colorimetric sensor that ultraviolet lamp triggers in embodiment 1 measures various concentration copper ion standard solution
Color diagram；
Fig. 8 is that the copper ion colorimetric sensor that ultraviolet lamp triggers in embodiment 1 measures various concentration copper ion standard solution
Standard curve；
Fig. 9 is that the light-triggered copper ion colorimetric sensor of the sun measures various concentration copper ion standard solution in embodiment 2
Color diagram；
Wherein, 1 is shell, and 2 be light source, and 3 be reaction vessel, and 4 be camera, and 3a is the reaction vessel of embodiment 1, and 3b is real
Apply the reaction vessel of example 2.
Specific implementation mode
The copper ion colorimetric method sensor of the utility model is made further by following embodiment and verification test
It is bright.
Embodiment 1
As shown in Figure 1, Figure 3, the copper ion colorimetric method sensor of the present embodiment includes shell 1, reaction vessel 3 and light source 2；
Light source 2 and reaction vessel 3 are set in shell 1；
Reaction vessel 3 is whiteware cuvette 3a, and reaction vessel 3 is located at bottom in shell 1, and the light source 2 is located at anti-
Answer 3 top of container；
Whiteware cuvette 3a is equipped with 6 reactive tanks 5；
Light source 2 is the ultraviolet lamp tube of 254nm, power 10W.
Embodiment 2
As shown in figure 4, the copper ion colorimetric method sensor of the present embodiment difference from example 1 is that：This implementation
The reaction vessel 3 of example is transparent glass colorimetric cylinder 3b, and transparent glass colorimetric cylinder 3b is equipped with 6 reactive tanks 5.
Embodiment 3
The copper ion colorimetric method sensor of the present embodiment difference from example 1 is that：The light source 2 of the present embodiment is
The sun.
Embodiment 4
As shown in Fig. 2, the copper ion colorimetric method sensor of the present embodiment difference from example 1 is that：This implementation
It is additionally provided with camera 4 above the reaction vessel of example, the color for recording reaction solution in reaction vessel 3.
It, can be to copper ion in sample to be tested using color developing agent using the copper ion colorimetric method sensor of the utility model
Content is measured.Color developing agent includes quantum dot solution, 3,3', 5,5'- tetramethyl biphenyl amine aqueous solutions and acidic buffer, wherein
Quantum dot solution is the solution of CdTe/CdS quantum dots, ZnSe/ZnS quantum dots or Mn/ZnS quantum dots, 3,3', 5,5'- tetramethyls
The solvent of base biphenyl amine aqueous solution is dimethyl sub-maple, and acidic buffer is citrate buffer solution, hac buffer, phosphoric acid buffer
The pH value of solution or glycine-HCI buffer solution, acidic buffer solution is 3-6.
The application method of the utility model copper ion colorimetric method sensor includes the following steps：
(1) it mixes：Color developing agent, testing liquid are uniformly mixed and obtain mixed liquor, mixed liquor is placed in the anti-of reaction vessel 3
It answers in slot 5, the volume of the mixed liquor is 100~600 μ L；
(2) it reacts：After irradiating 3 3~5min of reaction vessel with light source 2, stops irradiation and stop reaction；
(3) it records：With the color after the record reaction of camera 4.
The sensor operating principles (as shown in Figure 5) of the utility model：In an acidic solution, TMB is irradiated with
It can be oxidized to the oxide (TMBox) of TMB afterwards, solution becomes blue from colourless；Under same radiation parameter, quantum dot is again
TMBox can be reduced to TMB, suitable in quantum dot dosage, solution will present colourless again, reach a dynamic
Balance；And the presence of copper ion can reduce the reducing power of quantum dot so that a part of TMBox can not be reduced, and copper ion is dense
Degree is higher, and remaining TMBox is more, and the blue of solution is deeper, can determine whether the dense of copper ion by observing solution colour
Degree.Based on above-mentioned principle, under the irradiation of light source 2, the sample to be tested and color developing agent of different content of copper ion are in reaction vessel 3
Reaction, obtain different colours in a linear relationship, according to camera 4 record copper ion standard solution reaction after color with it is to be measured
Color after example reaction is compared, and obtains the content of copper ion in sample to be tested.
Using the optical filter of different wave length on xenon source, TMB citric acid solutions are irradiated, are found only
There is the ultraviolet light of 350nm wavelength below that can aoxidize TMB, therefore the utility model uses and contains 350nm wavelength below
Light source is measured content of copper ion.Following operation is carried out using the copper ion colorimetric method sensor in embodiment 1.
Into one, the study show that, the utility model copper ion colorimetric sensor has linear response for copper ion.
It is as follows：
(1) accurate formulation ion concentration is respectively 2,5,10,20,30,40,50 μM of copper ion standard solution；
(2) by 20 μ L, 1 μM of CdTe/CdS quantum dots, the TMB solution of 20 μ L, 1500mg/L, the copper of 20 μ L various concentrations
Ion and a certain amount of citrate buffer solution solution (0.1M, pH4.5) are respectively placed in the different holes of color board, mixing, overall
Product is 400 μ L.
(3) color board equipped with above-mentioned developing solution is placed in irradiation reaction 3min under the ultraviolet lamp of 254nm wavelength, taken out,
With the color after digital camera record reaction, as shown in fig. 7, a to f refers respectively to be not added with copper ion, copper ion concentration is
0.1,0.25,0.5,1,1.5,2,2.5 μM when corresponding system color.
(4) ultraviolet absorption peak of the solution in 800-300nm wave-length coverages is measured with ultraviolet specrophotometer.Selection
Absorption peak at 650nm is as detection signal, as shown in fig. 6, the size of ultraviolet absorption peak is related with ion concentration, a to f difference
Refer to being not added with copper ion, copper ion concentration 0.1,0.25,0.5,1,1.5,2,2.5 μM when corresponding ultraviolet absorption peak.
Linear relationship as shown in figure 8, copper ion in 0.1-2.5uM concentration ranges, in a linear relationship, linear equation Y
=0.18187X+0.01328 (unit μM), related coefficient 0.99516.
Therefore, the application method of the copper ion colorimetric method sensor of the present embodiment carries out according to the following steps：
(1) it mixes：Color developing agent, testing liquid are uniformly mixed and obtain mixed liquor, mixed liquor is placed in the anti-of reaction vessel
It answers in slot, the volume of the mixed liquor is 100~600 μ L；
(2) it reacts：After 3~5min of light source irradiation reaction vessel, stops irradiation and stop reaction；
(3) it detects：Mixed liquor absorbance at 650nm after reacting is measured with ultraviolet specrophotometer；
(4) it calculates：The absorbance that standard solution is measured according to step (3) makes the standard curve of various concentration copper ion,
Then the concentration of copper ion in sample solution is calculated with the absorbance for measuring sample solution according to standard curve；
The testing liquid is standard solution or sample solution；
The light source is the ultraviolet lamp of 254nm wavelength.
Because also containing 350nm wavelength below in sunlight, we can be directly using sunlight as triggering light
Source；Color board containing color developing agent is placed directly under sunny sunlight and is irradiated to measure the copper ion of various concentration.Using reality
It applies the copper ion colorimetric method sensor in example 4 and carries out following operation.
It is as follows：
(1) accurate formulation ion concentration is respectively 5 μM, 10 μM, 20 μM, 40 μM and 100 μM of copper ion standard solution；
(2) by 30 μ L, 1 μM of CdTe/CdS quantum dots, the TMB solution of 20 μ L, 1500mg/L, the copper of 20 μ L various concentrations
Ion and a certain amount of citrate buffer solution solution (0.1M, pH4.5) are respectively placed in the different holes of color board, mixing, overall
Product is 400 μ L.
(3) color board equipped with above-mentioned developing solution is placed in irradiation reaction 20min under sunlight, takes out, uses digital camera
Color after record reaction, as shown in figure 9, a to f refers respectively to be not added with copper ion, copper ion concentration 0,0.25,0.5,1,
2,5 μM when corresponding system color.
Therefore, the color after being reacted with copper ion standard solution by the color after the reaction by sample to be tested carries out pair
Than, you can rough judgement with the naked eye is carried out to copper ion concentration in sample to be tested, realizes that simple and quick measurement copper ion contains
The purpose of amount.
Although the foregoing describe specific embodiment of the present utility model, it will be appreciated by those of skill in the art that
These are merely examples, and the scope of protection of the utility model is defined by the appended claims.Those skilled in the art
Member can make these embodiments numerous variations or repair under the premise of without departing substantially from the principles of the present invention and essence
Change, but these change and modification each fall within the scope of protection of the utility model.
Claims (5)
1. a kind of copper ion colorimetric method sensor, it is characterised in that：The sensor includes shell, reaction vessel and light source；
The light source and reaction vessel are set in shell；
The reaction vessel is located at bottom in shell, and the light source is located above reaction vessel；
The reaction vessel is equipped with multiple reactive tanks；
The light source is the light source of the ultraviolet light less than 350nm containing wavelength.
2. copper ion colorimetric method sensor according to claim 1, it is characterised in that：It is additionally provided with phase above the sensor
Machine.
3. copper ion colorimetric method sensor according to claim 1, it is characterised in that：The reactor is white or transparent
's.
4. copper ion colorimetric method sensor according to claim 3, it is characterised in that：The reactor is whiteware ratio
Colour table or transparent glass colorimetric cylinder.
5. copper ion colorimetric method sensor according to claim 1, it is characterised in that：The light source is the ultraviolet of 254nm
Fluorescent tube, power 10W.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201721429029.7U CN207779891U (en) | 2017-10-31 | 2017-10-31 | Copper ion colorimetric method sensor |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201721429029.7U CN207779891U (en) | 2017-10-31 | 2017-10-31 | Copper ion colorimetric method sensor |
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| Publication Number | Publication Date |
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| CN207779891U true CN207779891U (en) | 2018-08-28 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201721429029.7U Expired - Fee Related CN207779891U (en) | 2017-10-31 | 2017-10-31 | Copper ion colorimetric method sensor |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107655844A (en) * | 2017-10-31 | 2018-02-02 | 中国检验检疫科学研究院 | Developer, copper ion colorimetric method sensor and its application method and purposes for copper ion colorimetric method |
-
2017
- 2017-10-31 CN CN201721429029.7U patent/CN207779891U/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107655844A (en) * | 2017-10-31 | 2018-02-02 | 中国检验检疫科学研究院 | Developer, copper ion colorimetric method sensor and its application method and purposes for copper ion colorimetric method |
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| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20180828 Termination date: 20211031 |
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| CF01 | Termination of patent right due to non-payment of annual fee |