CN109540842B - Double-fluorescence signal and water quality monitoring probe based on LED light source and use method - Google Patents
Double-fluorescence signal and water quality monitoring probe based on LED light source and use method Download PDFInfo
- Publication number
- CN109540842B CN109540842B CN201910035691.1A CN201910035691A CN109540842B CN 109540842 B CN109540842 B CN 109540842B CN 201910035691 A CN201910035691 A CN 201910035691A CN 109540842 B CN109540842 B CN 109540842B
- Authority
- CN
- China
- Prior art keywords
- light source
- led light
- signal
- signals
- module
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/47—Scattering, i.e. diffuse reflection
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
Landscapes
- Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
Abstract
The invention discloses a double-fluorescent signal and turbidity water quality monitoring probe based on an LED light source and a use method thereof, and belongs to the technical field of online water quality monitoring. The fluorescent light-scattering lamp comprises a shell, an internal support, an optical component and an electronic circuit system, wherein the internal support, the optical component and the electronic circuit system are arranged in the shell, the optical component provides a light source and converts fluorescent light or fluorescent and scattering light signals into electric signals, and the electronic circuit system processes the electric signals and outputs the processed electric signals. The probe of the invention simultaneously excites and detects protein fluorescence and humus fluorescence through the deep ultraviolet LED, calculates the ratio between the two signals to reflect the composition types and concentration changes of the soluble organic matters in the water body, and can also reflect the turbidity of the water body through the scattered light of the blue light LED to assist in judging the pollution condition of the water body.
Description
Technical Field
The invention relates to an online water quality monitoring technology in the field of environmental protection, in particular to a water quality monitoring probe for detecting soluble organic matters by a fluorescent method based on an LED light source and detecting turbidity by assisting scattered light.
Background
The soluble organic matters (dissolved organic matter, DOM) existing in the natural water body mainly comprise macromolecular proteins, humic acid with medium molecular weight, fulvic acid and other small molecular substances. In the drinking water treatment process, DOM can generate disinfection byproducts with cancerogenic action in the chlorination disinfection process; in the terminal pipe network, DOM can provide a carbon source for the growth of microorganisms in the pipeline to form a biological film; in natural water environments, DOM is a major contributor to chemical oxygen demand (Chemical Oxygen Demand, COD) and affects the migratory conversion of other contaminants.
Methods for analytically detecting DOM concentration levels mainly include chemical methods and spectroscopic methods. Wherein the chemical method mainly comprises a chemical oxygen demand test and a total organic carbon test, and the spectroscopic method mainly comprises an ultraviolet-visible absorbance method and a fluorescence spectroscopic method. Although widely adopted in national or industry standards, the chemical method has the advantages of complex structure, larger volume, high price, long test period, chemical reagent requirement, secondary chemical pollution and high operation and maintenance and waste liquid treatment cost of on-line monitoring equipment; the spectrometry has the advantages of quick sensitivity, no need of chemical reagents and the like. The ultraviolet absorbance method mainly detects aromatic structures such as benzene rings and derivatives thereof in water, and macromolecular proteins, humic acid, fulvic acid and some small molecular compounds containing benzene rings in water can be detected by the ultraviolet absorbance method; the fluorescence of the soluble organic matters mainly comprises protein fluorescence, humus fluorescence, chlorophyll and other pigment fluorescence. The emission wavelength range of the protein fluorescence is 310-370nm; the emission wavelength range of the humus fluorescence is 400-500nm; the intracellular substances released by blue algae death comprise protein fluorescence, humus fluorescence and chlorophyll fluorescence, and the peak wavelength of the chlorophyll fluorescence is about 685 nm. The protein fluorescent signal is mainly used for detecting substances containing phenol or aniline structures in water and comprises macromolecular proteins, humus, fulvic acid, tryptophan and tyrosine and some small molecular compounds containing phenol or aniline structures; humic substances fluorescence mainly detects substances containing polycyclic aromatic structures such as humic acid, fulvic acid, naphthol, naphthylamine, quinine, pterin and the like in water. To a certain extent, ultraviolet absorbance UV254, protein fluorescence and humus fluorescence can reflect the soluble organic species and concentration levels thereof in water. With the rapid development of intelligent water affairs in recent years, water environment monitoring industry urgent needs water quality sensors or monitoring equipment with small volume, low cost, easy maintenance and the like so as to realize wide arrangement of monitoring points. Under the background, the online monitoring of DOM concentration level by the spectrometry is gradually accepted by the water quality industry, and is widely applied to a series of 'river length system' water quality monitoring projects.
Currently, commercially applied spectroscopy equipment reflecting DOM concentration levels is mainly based on ultraviolet absorbance or ultraviolet-visible absorbance spectroscopy. For example, using a low pressure mercury lamp as a light source, ultraviolet absorbance at a wavelength of 254nm (UV 254) was tested as an alternative indicator of chemical oxygen demand; by adopting a pulse xenon lamp light source, analysis and detection of indexes such as nitrate concentration, DOM concentration, turbidity and the like are realized by testing ultraviolet-visible absorption luminosity of a wave band of 200-750 nm. However, these spectroscopy water quality monitoring devices or probes based on low-pressure mercury lamps or pulsed xenon lamp light sources still have the problems of large volume and high power consumption.
The patent 201410502662.9 of China patent application publication No. 201510738667.6 discloses an ultraviolet fluorescence three-signal water quality sensor with a single UV-LED as a light source and application thereof, wherein the publication No. 2014 is 12 and 10, the application publication No. 201510738667.6 discloses an ultraviolet fluorescence two-signal water quality monitoring device with an LED as a light source, the application publication No. 2015 is 12 and 23, the application publication adopts an ultraviolet LED as a light source, a photodiode at the relative position of a light path is adopted to detect ultraviolet absorbance value, and a bandpass filter and a photodiode at the position perpendicular to the light path of the ultraviolet LED are adopted to detect two fluorescence signals such as proteins or humus. However, the above invention still has the following problems to be overcome: 1) Natural light interferes with fluorescent signal detection, if a light shield is added on the probe, water circulation is affected, electronic circuit design needs to be further optimized, natural light interference is eliminated, and signal to noise ratio is improved; 2) The U-shaped probe or the flow cell is designed, so that the quartz optical window is difficult to clean after being polluted by biological films and the like; 3) Some natural water bodies show higher ultraviolet absorbance or chemical oxygen demand values by receiving humic acid flushed by surrounding mountain soil, but the biological availability of organic matters in the water bodies is lower, and the water bodies are not black and odorous water bodies or polluted water bodies; 4) Under the influence of rainfall events, soil particles and humus substances in the soil are flushed into a river of a person, so that turbidity is increased, chemical oxygen demand is high, and ultraviolet absorbance or absolute value increase of a humus fluorescent signal caused by the rainfall events cannot be classified as a water pollution event because humus is low in bioavailability and harmless to the water environment, and turbidity needs to be detected while the fluorescent signal is detected. Research shows that the ratio of protein fluorescence to humus fluorescence signals of a water body affected by urban sewage discharge is obviously higher than that of a water body which is not polluted due to the abundance of microorganisms; in addition, tyrosine and tryptophan constituting proteins have key fluorescent structures of phenol and aniline derivatives, and phenol or aniline and its derivatives are often contained in some chemical pollution events, so that a high fluorescent signal of proteins is caused.
The application of Chinese patent application number 201521042180.6, publication date is 2016, 6 and 1, discloses an immersed type oil-in-water monitoring probe, which comprises a shell with a closed structure, an ultraviolet light source, a fluorescent filter, a photoelectric detector, a main board and a driving circuit board, wherein the ultraviolet light source, the fluorescent filter, the photoelectric detector, the main board and the driving circuit board are arranged in the shell, and the front end of the shell is opened and is connected with a glass window in an embedded mode at the opening; the inner side of the glass window is correspondingly provided with a photoelectric detector and an ultraviolet light source, the photoelectric detector and the ultraviolet light source are connected to a driving circuit board, and the driving circuit board is connected with the main board; the waterproof connector is arranged on the shell, and has the advantages that (1) the high-frequency modulation light source is adopted, so that the environment light interference resistance is stronger; (2) The design of a large-aperture light path is adopted, so that the luminous flux is larger, and the interference capability of suspended matters in a water sample is stronger; (3) The light path is simpler, the cost is lower, and the light path is suitable for wide application and popularization. However, the invention is characterized in that a plurality of LED light sources are annularly distributed around one photodiode, and one probe can only measure fluorescent signals in one wavelength range. According to the principle of fluorescence, for a fluorescent substance, the emission wavelength of fluorescence is fixed, and electrons are caused to transit to a first excited state or higher, the electrons in the higher excited state fall back to the first excited state through a molecular relaxation process, which does not generate fluorescence, and only the process of returning to the ground state from the first excited state emits fluorescence, depending on the energy difference between the first excited state and the ground state, i.e., regardless of the wavelength of the excitation light source. It is therefore unnecessary to use a multiple wavelength uv light source for excitation of fluorescence; the probe design can only be used for one fluorescence signal, can not be used for simultaneously measuring two fluorescence signals of protein fluorescence and humus fluorescence, and can not be used for obtaining the ratio of the two fluorescence signals. In addition, the probe disclosed by the invention cannot analyze the turbidity in water, so that whether the change of the fluorescent signal in water is influenced by the turbidity cannot be judged.
The Chinese patent application number 201510166525.7, the application with publication date 2015, 6 and 24, discloses an integrated probe type photoelectric water quality multi-parameter online measurement system, which comprises a control and data acquisition unit and an optical system unit; the control and data acquisition unit comprises an upper computer, a control and data acquisition unit and an acquisition device; the optical system unit comprises a light source and a light path, wherein the light source comprises a left light source and a right light source, and the left light source is an LED array formed by a group of LED light sources with different wavelengths; the ultraviolet LED light source and the near infrared LED light source form a right light source; the upper computer controls the light source to emit light with different wavelengths required by the control and data acquisition unit to irradiate the water body to be detected, and excites the substances in the water body to be detected to emit fluorescent and scattered light signals; the invention has the advantages that: no chemical reagent is needed for direct measurement, the detection data is fast, and complicated steps are avoided; meanwhile, the classification and concentration detection of various algae in water quality, and the on-line detection of hydrocarbon content and turbidity are completed; can carry out independent accurate detection and meet the requirements of different environmental monitoring. However, the invention mainly distinguishes the algae type in water and reflects the algae concentration by testing chlorophyll or other pigment forms, the light source used is a visible light LED combined light source with the wavelength of more than 370nm, and the visible light with the wavelength of more than 370nm can not be used for exciting protein substances and humus substances in water to generate fluorescence due to the lower energy, and it is noted that the filter B502 in the embodiment of the invention can be a 360nm filter for hydrocarbon testing, which is contrary to the fluorescence principle, because of energy loss in the electronic transition process, the emission wavelength of fluorescence is necessarily larger than the excitation wavelength, and is usually larger than the excitation wavelength by more than 30 nm.
In view of the above, it is necessary to design a water quality monitoring probe for simultaneously monitoring protein fluorescence, humus fluorescence and turbidity signals.
Disclosure of Invention
1. Problems to be solved
Aiming at the technical requirements that the water environment monitoring industry needs to rapidly and sensitively monitor the concentration of soluble organic matters in water and accurately reflect the water pollution condition, the invention aims to provide a double-fluorescence signal and turbidity water quality monitoring probe based on an LED light source and a use method thereof.
2. Technical proposal
In order to solve the problems, the technical scheme adopted by the invention is as follows:
the double-fluorescence signal and turbidity water quality monitoring probe based on the LED light source consists of a shell, an internal support, an optical component and an electronic circuit system, wherein the shell comprises a front cover with waterproof and light-transmitting functions, a barrel for accommodating and supporting a photoelectric device and a tail cover with a cable, one end of the shell is provided with a quartz plate light outlet, and the shell is internally provided with the internal support, the optical component and the electronic circuit system; the internal support is used for bearing an optical component and an electronic circuit system and fixing positions and angles, wherein the optical component comprises an LED light source and two groups of detection components, and the detection components convert two fluorescent or two fluorescent plus blue light scattering light signals into electric signals; the electronic circuit system processes the received electric signals and outputs the processed electric signals.
Furthermore, the double fluorescent signals and turbidity water quality monitoring probe based on the LED light source can detect protein fluorescent signals and humus fluorescent signals by exciting soluble organic matters in water through deep ultraviolet light, and can detect turbidity by assisting blue light to irradiate particles in water to generate stray light.
As a further illustration of the invention, the front cover comprises an annular base, a sealing ring and a quartz plate, wherein the side surface of the annular base is provided with internal threads and the bottom is provided with a groove for embedding the sealing ring; the cylinder body comprises a sealing ring, front-end external threads, an annular support and rear-end internal threads; the tail cover comprises a sealing ring, an annular tail cover main body, a sealing gasket, a hollow screw and a cable; the annular base is in sealing connection with the front end of the cylinder body through threads and the sealing ring, so that the sealing ring on the groove of the annular base is extruded by the quartz plate, and waterproof and light-transmitting sealing is realized.
Furthermore, the LED light source is formed by compositely packaging a deep ultraviolet LED chip with the center wavelength of 280+/-10 nm and a blue LED chip with the center wavelength of 465+/-10 nm on the same base, and a quartz lens is packaged above the chip base to concentrate light, so that the light emitting angle of the LED light source is smaller than 30 degrees, and the deep ultraviolet LED chip and the blue LED chip are respectively connected with respective driving circuits by adopting respective independent pins or common anode or common cathode pins, so that independent switching control is realized, and the LED light source is controlled to output blue light or deep ultraviolet light in a time-sharing multiplexing mode.
Furthermore, the LED light source adopts a deep ultraviolet LED chip with the center wavelength of 280+/-10 nm, and a quartz lens is packaged above the LED chip for condensing light, so that the light emitting angle of the LED light source is smaller than 30 degrees.
Furthermore, the two sets of detection components are a first detection component and a second detection component, wherein the first detection component is formed by packaging a band-pass filter A and a photodiode A, and the second detection component is formed by packaging a band-pass filter B and a photodiode B.
Further, the wavelength range of the band-pass filter A is 330-370nm, the wavelength range of the band-pass filter B is 400-500nm, the cut-off rate of the band-pass filter A and the band-pass filter B to the light intensity outside the band-pass wavelength range is more than 99.9%, the photodiode A and the photodiode B are silicon photodiodes with higher linear response to ultraviolet-visible light in the range of 300-500 nm so as to realize detection of fluorescence light intensity or scattered light intensity, and the band-pass filter is fixed above the photodiodes.
Furthermore, the positions of the LED light source and the two groups of detection assemblies are fixed through the internal support, the two groups of detection assemblies are respectively positioned at two sides of the LED light source, an included angle alpha between the axes of the two groups of detection assemblies and the axis of the LED light source is 45+/-15 degrees, and the axis of the LED light source and the axes of the two groups of detection assemblies are intersected at the outer side of the quartz plate.
Furthermore, the positions of the LED light source and the two groups of detection components are fixed through the internal support, the LED light source and the two groups of detection components are respectively positioned at three vertexes A, B and C at the bottom of the tetrahedron, the axes of the three are intersected at an upper vertex D of the tetrahedron, namely, the included angle of any two lines of the three edge lines AD, BD and CD is between 45 and 135 degrees, and the vertex D is positioned at the outer side of the quartz plate.
Further, the electronic circuit system comprises a singlechip, a power supply module, a photoelectric signal amplifying circuit, an AD analog-to-digital conversion module and a communication module; the photoelectric signal amplifying circuit preferably adopts a phase-locked amplifying technology, and further comprises a photoelectric signal operational amplifier module, a band-pass filtering module, a phase-sensitive detection module and a low-pass filtering module for realizing phase-locked amplification.
The power module supplies power for all components of the electronic circuit system. The singlechip controls the driving circuit, the driving circuit controls the LED light source to switch according to the frequency of 10 Hz-10 kHz, the organic matters in the water body are irradiated to generate fluorescence or scattered light signals with the same frequency, the deep ultraviolet light excites the soluble organic matters in the water to generate protein fluorescence or humus fluorescence with the same frequency, and the blue light irradiates the particulate matters in the water to generate weak stray light with the same frequency. The photoelectric signal operational amplifier module processes the electric signal output by the photoelectric diode by adopting a photovoltaic mode with zero bias voltage, and the operational amplifier adopts a transimpedance amplification design, and the electric signal output by the photoelectric signal operational amplifier module is processed by the band-pass filter module and then is input to the phase-sensitive detection module and is compared with a reference signal with the same frequency as the LED light source switch, which is sent by the singlechip; the phase-sensitive detection module inputs the electric signal into the low-pass filtering module, the obtained signal is converted into a digital signal by the AD analog-to-digital conversion module and is input into the singlechip, and the singlechip is communicated with the upper computer.
Furthermore, the blue light scattered light for detecting turbidity and the humus fluorescent signal are amplified and processed in the same way by time division multiplexing; only the fluorescent signal or scattered light signal with specific frequency is processed by the phase sensitive detection module and then changed into direct current, the direct current can pass through the low-pass filtering module, and the signal generated by natural light is still an alternating current signal after passing through the phase sensitive detection module and is eliminated by the low-pass filtering module; the obtained signals are converted into digital signals through an AD analog-to-digital conversion module and are input into a singlechip, and the singlechip adopts a MODBUS-RTU communication protocol to carry out data transmission with an upper computer.
Furthermore, the LED light source is formed by compositely packaging a deep ultraviolet LED chip with the center wavelength of 280+/-10 nm and a blue LED chip with the center wavelength of 465+/-10 nm, the LED light source is controlled to alternately output blue light or deep ultraviolet light in a time-sharing multiplexing mode, and the set frequency of the LED light source switch is 10Hz to 10kHz.
The use method of the double fluorescent signal and turbidity water quality monitoring probe based on the LED light source comprises the following steps:
(1) Immersing the probe into a water sample to be detected;
(2) The power supply module is turned on, the singlechip controls the driving circuit, the driving circuit controls the LED light source to switch according to the frequency of 10Hz to 10kHz, and when the LED light source is a light source formed by compositely packaging a deep ultraviolet LED chip with the center wavelength of 280+/-10 nm and a blue LED chip with the center wavelength of 465+/-10 nm, the LED light source is controlled to alternately output blue light or deep ultraviolet light in a time-sharing multiplexing mode;
(3) When the LED light source outputs deep ultraviolet light with the center wavelength of 280+/-10 nm, the deep ultraviolet light irradiates soluble organic matters in a water sample through a quartz plate and generates protein fluorescence and/or humus fluorescence signals, the protein fluorescence signals are transmitted to the photodiode A through the band-pass filter A, and the humus fluorescence signals are transmitted to the photodiode B through the band-pass filter B;
(4) When the LED light source outputs blue light, the blue light irradiates particles in a water sample through the quartz plate to form scattered light signals, and the scattered light signals of the blue light are transmitted to the photodiode B through the band-pass filter B;
(5) Photodiode a and/or photodiode B converts the optical signal into an electrical signal for output;
(6) The photoelectric signal operational amplifier module processes the electric signals output by the photodiode A and/or the photodiode B, amplifies the electric signals and transmits the amplified electric signals to the band-pass filter module; the band-pass filtering module processes the electric signal and inputs the electric signal to the phase-sensitive detection module, then transmits the electric signal to the low-pass filtering module, and then converts the electric signal into a digital signal through the AD analog-to-digital conversion module;
(7) The singlechip collects the digital signals and communicates with the upper computer to obtain on-line monitoring data of protein fluorescence, humus fluorescence and scattered light intensity signals, and calculates the ratio of the protein fluorescence signals to the humus fluorescence signals.
3. Advantageous effects
Compared with the prior art, the invention has the beneficial effects that:
(1) The double-fluorescence signal and turbidity water quality monitoring probe based on the LED light source has the advantages that the same deep ultraviolet LED is adopted for excitation, two groups of photodiodes and band-pass filters are adopted for respectively and simultaneously detecting protein fluorescence signals and humus fluorescence signals, and compared with the existing on-line spectrum monitoring equipment of a xenon lamp or mercury lamp light source, the double-fluorescence signal and turbidity water quality monitoring probe based on the LED light source has the advantages of being small in size, low in power consumption, low in cost, simple in structure and the like.
(2) The dual-fluorescence signal and turbidity water quality monitoring probe based on the LED light source has the advantages that the characteristic that the LED chip can be rapidly and frequently switched is utilized to work at a set frequency, and the fluorescent signal with a specific frequency is excited to be generated, so that the weak fluorescent signal with the specific frequency can be demodulated from a stronger natural light interference signal by using a phase-locked amplification technology, and the dual-fluorescence signal and turbidity water quality monitoring probe has the advantage of high anti-interference capability.
(3) The dual-fluorescence signal and turbidity water quality monitoring probe based on the LED light source has the advantages that the fluorescence signal is detected, and meanwhile, the turbidity signal is detected, namely, when the blue light LED chip works, a detection device and a circuit of the humus fluorescence signal can be used for detecting blue light stray light caused by particles in water, so that time-sharing multiplexing of light paths is realized.
(4) The dual-fluorescence signal and turbidity water quality monitoring probe based on the LED light source has the advantages that the front cover of the probe only adopts one quartz plate as a common light window of the LED light source and two sets of photodiodes, the waterproof sealing and light transmission effects are realized, and the cleaning and the maintenance are more convenient.
(5) Compared with the ultraviolet spectroscopy water quality monitoring probe, the LED light source-based dual-fluorescence signal and turbidity water quality monitoring probe has the advantages that protein fluorescence, humus fluorescence and turbidity signals are monitored simultaneously, whether the water quality monitoring probe is polluted by domestic sewage or phenolic compounds can be judged according to the relative ratio of the protein fluorescence and the humus fluorescence signals, and the turbidity signals can assist in judging whether the rise of the fluorescence signals in the water body is from events such as rainfall.
Drawings
FIG. 1 is an exploded view of a dual fluorescent signal and turbidity water quality monitoring probe based on an LED light source;
FIG. 2 is a cross-sectional view of a dual fluorescent signal and turbidity water quality monitoring probe based on an LED light source of the present invention;
FIG. 3 is a first spatial layout design of the front-end optics of the probe of the present invention;
FIG. 4 shows a first embodiment of the probe insert holder according to the invention in (a) top view, (b) bottom view and (c) side view;
FIG. 5 is a diagram of an electronic circuitry architecture of the present invention;
FIG. 6 is a schematic diagram of the principle of fluorescence and turbidity detection according to the present invention: (a) A schematic drawing of deep ultraviolet excited protein fluorescence and humus fluorescence, and a schematic drawing of scattered light detection turbidity;
FIG. 7 is a linear regression curve of fluorescence and scattered light signals from a probe of the present invention versus standard solution: a linear regression curve between (a) the protein fluorescent signal and tryptophan concentration, (b) the humus fluorescent signal and quinine sulfate concentration, and (c) the scattered light signal and turbidity;
FIG. 8 is a second spatial layout design of the probe optic of the present invention: (a) A front top view, (b) a schematic view of the spatial vertical relationship of the LED light source and the two photodiodes;
FIG. 9 is a graph showing the continuous monitoring data of the protein fluorescence, humus fluorescence and blue light scattering intensity of a hydrazine sulfate turbidity reagent added with 4NTU units after a certain city river water sample is treated by a 0.45 μm filter membrane;
FIG. 10 is the continuous monitoring data of the fluorescence of proteins, the fluorescence of humus and the scattered light intensity of blue light of a hydrazine sulfate turbidity reagent added with 4NTU units after the effluent of a secondary sedimentation tank of a domestic sewage treatment plant is treated by a 0.45 mu m filter membrane.
In the figure:
1. a front cover; 101. an annular base; 102. a first sealing ring; 103. quartz plates; 2. a cylinder; 201. a second sealing ring; 202. front external threads; 203. a ring support; 204. internal threads at the rear end; 3. a tail cover; 301. a third sealing ring; 302. an annular tail cap body; 303. a sealing gasket; 304. a hollow screw; 305. a cable; 4. an inner support; 401. a central bore; 402. a side hole; 5. detecting a first component; 501. a bandpass filter A; 502. a photodiode A; 6. a second detection component; 601. a band-pass filter B; 602. a photodiode B; 7. an LED light source; 8. an electronic circuitry; 801. a single chip microcomputer; 802. a power module; 803. the photoelectric signal operational amplifier module; 804. a band-pass filtering module; 805. a phase sensitive detection module; 806. a low pass filtering module; 807. an AD analog-to-digital conversion module; 808. a communication module; 809. a temperature sensor; 810. and a driving circuit.
Detailed Description
The invention is further described below in connection with specific embodiments.
Example 1
As shown in fig. 1, the dual fluorescent signal and turbidity water quality monitoring probe based on the LED light source of the present embodiment comprises a housing, an inner support 4, an optical component and an electronic circuit system 8.
The shell comprises a front cover 1 with waterproof and light-transmitting functions, a barrel 2 for accommodating and supporting photoelectric devices and a tail cover 3 with a cable 305, the optical component comprises two detection assemblies and an LED light source 7 which is formed by compositely packaging a deep ultraviolet LED chip with the center wavelength of 280+/-10 nm and a blue LED chip with the center wavelength of 465+/-10 nm on the same base, and the internal support 4 provides support for the optical component and fixes the position and the angle; the electronic circuit system 8 controls the switch of the compound packaging LED light source 7, the amplification of the signals of the photodiode A502 and the photodiode B602, data communication and the like; the probe can detect protein fluorescent signals and humus fluorescent signals simultaneously by exciting soluble organic matters in water through deep ultraviolet light, and can detect turbidity by blue light irradiating particles in water to generate stray light.
As shown in fig. 1 and 2, the front cover 1 includes an annular base 101, a first sealing ring 102 and a quartz plate 103, wherein the side surface of the annular base 101 is provided with internal threads and the bottom is provided with a groove for embedding the sealing ring; the cylinder body 2 comprises a second sealing ring 201, front-end external threads 202, an annular support 203 and rear-end internal threads 204; the tail cover 3 comprises a sealing ring III 301, an annular tail cover main body 302, a sealing gasket 303, a hollow screw 304 and a cable 305; the annular base 101 is in sealing connection with the front end of the cylinder body 2 through threads and the first sealing ring 102, so that the first sealing ring 102 on the groove of the annular base 101 is extruded by the quartz plate 103, and waterproof and light-transmitting sealing is realized. The main bodies of the front cover 1, the cylinder body 2 and the tail cover 3 are made of stainless steel materials and are processed by a numerical control lathe.
The two circular band-pass filters A501 and the band-pass filter B601 respectively adopt (i) a band-pass filter A501 with the wavelength of 330-370nm for detecting protein fluorescent signals and (ii) a band-pass filter B601 with the wavelength of 400-500nm for detecting humus fluorescent signals, wherein the cut-off rate of the two band-pass filters on light outside the band-pass wavelength range is more than 99.9%, the diameter is 8mm, and the thickness is 2.2mm.
The two photodiodes adopt a violet-blue light enhanced high-precision linear response silicon photodiode, the packaging form is TO-18 metal packaging, the photosensitive area is 3.7mm multiplied by 3.7mm, and as shown in fig. 2, a band-pass filter A501 and a band-pass filter B601 are respectively fixed above the photodiode A502 and the photodiode B602.
The deep ultraviolet and blue light composite packaged LED light source 7 is characterized in that a deep ultraviolet LED chip with a center wavelength of 280+/-10 nm and a blue light LED chip with a center wavelength of 465+/-10 nm are compositely packaged in a TO39 metal package, the ultraviolet LED chip and the blue light LED chip adopt independent anode pins and cathode pins respectively TO be connected with a driving circuit 810, a singlechip 801 controls the on-off of the driving circuit 810 of an LED by outputting high and low levels, independent switching control of the two LED chips is realized, a TO39 is packaged with a quartz condensing lens, and the light emitting angle of the TO39 is measured TO be about 7 degrees.
The position relationship between the deep ultraviolet and blue light combined LED light source 7 and the two groups of detection components is fixed through the internal support 4, as shown in fig. 3, the LED light source 7 is positioned in the middle, the axes of the detection components I5 and II 6 positioned at two sides are 45 degrees with the axis alpha of the LED light source 7, and the intersection of the axis of the LED light source 7 and the axes of the two groups of detection components on the outer side of the quartz plate 103 is ensured; the structure of the inner support 4 corresponding to the first design is shown in fig. 4, with a central hole 401 for the fixation of the LED light source 7 and two side holes 402 for the fixation of the bandpass filter and the photodiode.
The components in the electronic circuit system 8 are all welded on a printed circuit board, as shown in fig. 5, and include a singlechip 801, a power supply module 802, a photoelectric signal operational amplifier module 803, a band-pass filter module 804, a phase-sensitive detection module 805, a low-pass filter module 806, an AD analog-to-digital conversion module 807, a communication module 808 and a temperature sensor 809.
Wherein the power module 802 provides power to components of the electronic circuitry 8; the singlechip 801 outputs a digital signal to control the driving circuit 810, the driving circuit 810 controls the time-sharing multiplexing of a deep ultraviolet LED chip and a blue LED chip in the LED light source 7, the deep ultraviolet LED chip is switched on and off at the frequency of 1kHz within 0-499ms by taking 1s as a test period, the deep ultraviolet light of 1kHz is emitted, and the blue LED chip is switched on and off at the frequency of 1kHz within 500-999ms, and the blue light of 1kHz is emitted; wherein, the deep ultraviolet light excites the soluble organic matters in the water to generate protein fluorescence with the frequency of 1kHz, which can be detected by the photodiode A502 through the band-pass filter A501, the generated humus fluorescence can be detected by the photodiode B602 through the band-pass filter B601, and the weak blue light stray light with the frequency of 1kHz generated by the blue light irradiated on the particles in the water can be detected by the photodiode B602 through the band-pass filter B601, the principle of which is shown in figure 6; the photoelectric signal operational amplifier module 803 adopts a photovoltaic mode with zero bias voltage for the photoelectric diode, the operational amplifier adopts an OPA129 chip of TI company to carry out transimpedance amplification design, a channel for measuring protein fluorescent signals adopts 500MΩ resistance, a channel for measuring humus fluorescent signals and turbidity signals adopts 100MΩ resistance, and an output signal is processed by the band-pass filter module 804 and then is input into the phase sensitive detection module 805, and is subjected to frequency and phase comparison with a reference signal of 1kHz sent by the singlechip 801; the stray light of the detection turbidity and the fluorescence signal of the detection humus adopt the same signal amplifying and processing circuit in a time-sharing multiplexing mode; the fluorescent signal or stray light signal with the frequency of only 1kHz is processed by the phase sensitive detection module 805 and then is changed into direct current, the direct current can pass through the low-pass filtering module 806, and the signal generated by natural light is an alternating current signal and is eliminated by the low-pass filtering module 806; the obtained signals are converted into digital signals through an AD analog-to-digital conversion module 807 and are input into a singlechip 801; the band-pass filtering module 804 and the low-pass filtering module 806 both adopt NE5532 chips of TI company, the phase-sensitive detection module 805 adopts AD630 chips of TI company, and the singlechip 801 adopts STM32F103C8T6 chips; the singlechip 801 respectively carries out average processing on the values of the protein fluorescent signal and the humus fluorescent signal within 0-499ms and the turbidity signal within 500-999ms, the communication module 808 carries out TTL and RS485 bidirectional signal conversion, and the singlechip 801 and the upper computer carry out signal transmission by adopting a MODBUS-RTU communication protocol. The singlechip 801 monitors the temperature of the circuit board through the patch type temperature sensor 809 and performs temperature compensation calibration on the obtained signal.
If a series of tryptophan aqueous solution, quinine sulfate aqueous solution and hydrazine sulfate turbidity reagent with concentration are respectively used as calibration reagents, the protein fluorescent signal, humus fluorescent signal and turbidity signal of the probe are tested to obtain a linear regression curve as shown in figure 7, R 2 All greater than 0.99, indicating that there isGood accuracy.
Example 2
This embodiment is basically the same as embodiment 1 except that:
the front cover 1, the cylinder 2 and the tail cover 3 of the shell structure are formed by adopting POM plastics through numerical control lathe processing.
The design of the internal support 4 is shown in fig. 8, and the fluorescent detection device formed by the LED light source 7, the first detection component 5 and the second detection component 6 is respectively located at three vertexes A, B and C at the bottom of the right-angle tetrahedron, and the axes of the three vertexes intersect at the vertex D above the right-angle tetrahedron, that is, any two lines of AD, BD and CD are mutually perpendicular. The tail cover 3 with the cable 305 can adopt an M12-specification nylon bending-resistant fixed waterproof cable connector, and only a round hole with the diameter of 12mm is formed in the center of the annular tail cover main body 302, so that the sealing gasket 303 and the hollow screw 304 can be omitted.
The pre-operational amplifier of the photoelectric signal operational amplifier module 803 in the electronic circuit system 8 adopts an LMP7721 chip of TI company, and the band-pass filter module 804 and the low-pass filter module 806 both adopt OPA227 chips of TI company.
The two-sedimentation tank effluent of a river water sample of a city and a domestic sewage plant is treated by a 0.45 mu m filter membrane and then added with a hydrazine sulfate turbidity standard reagent of 4NTU units, and the two fluorescent signals based on the composite LED light source and the turbidity water quality monitoring probe are adopted for detection in the embodiment to obtain on-line monitoring data of 0-1000 seconds, as shown in figure 9. The protein fluorescence, humus fluorescence and scattered light intensity signals of the river water sample are 39, 90 and 261AU respectively, and the ratio of the protein fluorescence signal to the humus fluorescence signal is 0.43; the protein fluorescence, humus fluorescence and scattered light intensity signals of the secondary submerged water sample of the domestic sewage plant are respectively 99, 235 and 265AU, and the ratio of the protein fluorescence signal to the humus fluorescence signal is-0.42. The ratio of the protein fluorescent signal/humus fluorescent signal of the river water sample to that of the domestic sewage secondary sedimentation tank is similar, which shows that more biodegradable organic carbon exists in the river and a large amount of microorganisms are metabolized to produce protein extracellular polymers, which is consistent with the eutrophication state of the river.
Example 3
This embodiment is basically the same as embodiment 1 except that:
the LED light source 7 is a TO39 metal-packaged deep ultraviolet LED light source, the central wavelength of the LED light source is 280+/-10 nm, the singlechip 801 outputs digital signals TO control the driving circuit 810, the driving circuit 810 controls the LED light source 7 TO switch at 1kHz frequency TO emit deep ultraviolet light, the deep ultraviolet light excites soluble organic matters in water TO generate protein fluorescence or humus fluorescence at 1kHz frequency, and the detection component I5 and the detection component II 6 respectively realize detection of protein fluorescence and humus fluorescence signals. In this example, the haze was detected without using blue scattered light, which is a simplified design of example 1.
And respectively treating a river water sample of a certain city and effluent water of a secondary sedimentation tank of a domestic sewage plant through a 0.45 mu m filter membrane, adding a hydrazine sulfate turbidity standard reagent with a unit of 4NTU, and detecting by adopting a double fluorescent signal based on a composite LED light source and a turbidity water quality monitoring probe in the embodiment to obtain on-line monitoring data of 0-1000 seconds. The protein fluorescence signal and the humus fluorescence signal of the river water sample are 38 AU and 91AU respectively, and the ratio of the protein fluorescence signal to the humus fluorescence signal is 0.42; the protein fluorescence and humus fluorescence signals of the secondary submerged water sample of the domestic sewage plant are respectively 98 AU and 233AU, and the ratio of the protein fluorescence signal to the humus fluorescence signal is 0.42. The ratio of the protein fluorescent signal/humus fluorescent signal of the river water sample to that of the domestic sewage secondary sedimentation tank is similar, which shows that more biodegradable organic carbon exists in the river and a large amount of microorganisms are metabolized to produce protein extracellular polymers, which is consistent with the eutrophication state of the river.
Claims (9)
1. The utility model provides a two fluorescence signal and turbidity water quality monitoring probe based on LED light source, comprises shell, interior support (4), optical component and electronic circuit system (8), its characterized in that: the LED fluorescent lamp is characterized in that a quartz plate (103) is arranged at one end of the shell and used as a light outlet, an inner support (4), an optical component and an electronic circuit system (8) are arranged in the shell, the inner support (4) is used for bearing the optical component and the electronic circuit system (8), the optical component comprises an LED light source (7) and two groups of detection components, the light emitting angle of the LED light source (7) is smaller than 30 degrees, the positions of the LED light source (7) and the two groups of detection components are fixed through the inner support (4), the two groups of detection components are respectively positioned at two sides of the LED light source (7), an included angle alpha between the axes of the two groups of detection components and the axis of the LED light source (7) is 45+/-15 degrees, the axes of the LED light source (7) and the axes of the two groups of detection components are intersected at the outer side of the quartz plate (103), and the detection components convert two fluorescent or two fluorescent and blue light scattering light signals into electric signals; the electronic circuit system (8) processes the received electric signals and outputs the processed electric signals.
2. The dual fluorescent signal and turbidity water quality monitoring probe based on an LED light source of claim 1, wherein: the LED light source (7) is formed by compositely packaging a deep ultraviolet LED chip with the center wavelength of 280+/-10 nm and a blue LED chip with the center wavelength of 465+/-10 nm on the same base, a quartz lens is packaged above the chip base, the deep ultraviolet LED chip and the blue LED chip are respectively connected with respective driving circuits (810) by adopting respective independent pins or shared anodes or shared cathode pins, independent switching control is realized, and the LED light source (7) is controlled to output blue light or deep ultraviolet light in a time-sharing multiplexing mode.
3. The dual fluorescent signal and turbidity water quality monitoring probe based on an LED light source of claim 1, wherein: the LED light source (7) adopts a deep ultraviolet LED chip with the central wavelength of 280+/-10 nm, and a quartz lens is packaged above the LED chip.
4. The dual fluorescent signal and turbidity water quality monitoring probe based on an LED light source of claim 1, wherein: the two groups of detection components are a first detection component (5) and a second detection component (6), wherein the first detection component (5) is formed by packaging a band-pass filter A (501) and a photodiode A (502), and the second detection component (6) is formed by packaging a band-pass filter B (601) and a photodiode B (602).
5. The dual fluorescent signal and turbidity water quality monitoring probe based on an LED light source of claim 4, wherein: the wavelength range of the band-pass filter A (501) is 330-370nm, the wavelength range of the band-pass filter B (601) is 400-500nm, the cut-off rate of the band-pass filter A (501) and the band-pass filter B (601) to the light intensity outside the band-pass wavelength range is more than 99.9%, and the photodiode A (502) and the photodiode B (602) are silicon photodiodes with higher linear response to ultraviolet-visible light in the range of 300-500 nm.
6. The dual fluorescent signal and turbidity water quality monitoring probe based on an LED light source of claim 1, wherein: the positions of the LED light source (7) and the two groups of detection components are fixed through the internal support (4), the LED light source (7) and the two groups of detection components are respectively located at three vertexes A, B and C at the bottom of the tetrahedron, the axes of the three vertexes are intersected at an upper vertex D of the tetrahedron, namely the included angle of any two of the three edge lines AD, BD and CD is between 45 and 135 degrees, and the vertex D is located outside the quartz plate (103).
7. The dual fluorescent signal and turbidity water quality monitoring probe based on an LED light source of claim 1, wherein: the electronic circuit system (8) comprises a singlechip (801), a power supply module (802), an optoelectronic signal amplifying circuit, an AD analog-to-digital conversion module (807) and a communication module (808).
8. The dual fluorescent signal and turbidity water quality monitoring probe based on an LED light source of claim 7, wherein: the photoelectric signal amplifying circuit adopts a phase-locked amplifying technology and comprises a photoelectric signal operational amplifier module (803), a band-pass filtering module (804), a phase-sensitive detection module (805) and a low-pass filtering module (806); the power module (802) supplies power for all components of the electronic circuit system (8); the singlechip (801) controls the driving circuit (810), the driving circuit (810) controls the LED light source (7) to switch according to the frequency of 10 Hz-10 kHz, the organic matters in the water body are irradiated to generate fluorescence or scattered light signals with the same frequency, the photodiode receives light transmitted through the band-pass filter and converts the light into electric signals to be output, the photoelectric signal operational amplifier module (803) processes the electric signals output by the photodiode, the electric signals output by the photoelectric signal operational amplifier module (803) are processed by the band-pass filter module (804) and then are input into the phase-sensitive detection module (805), and the electric signals are compared with reference signals which are sent by the singlechip (801) and have the same frequency as the switching of the LED light source (7); the phase-sensitive detection module (805) inputs the electric signal into the low-pass filtering module (806), the obtained signal is converted into a digital signal by the AD analog-to-digital conversion module (807) and is input into the singlechip (801), and the singlechip (801) is communicated with the upper computer.
9. A method for using the dual fluorescent signal and turbidity water quality monitoring probe based on the LED light source according to any one of claims 1 to 8, characterized in that: the method comprises the following steps:
(1) Immersing the probe into a water sample to be detected;
(2) The power supply module (802) is turned on, the singlechip (801) controls the driving circuit (810), the driving circuit (810) controls the LED light source (7) to switch according to the frequency of 10Hz to 10kHz, and when the LED light source (7) is a light source formed by compositely packaging a deep ultraviolet LED chip with the center wavelength of 280+/-10 nm and a blue LED chip with the center wavelength of 465+/-10 nm, the LED light source (7) is controlled to alternately output blue light or deep ultraviolet light in a time-sharing multiplexing mode;
(3) When the LED light source (7) outputs deep ultraviolet light with the center wavelength of 280+/-10 nm, the deep ultraviolet light irradiates soluble organic matters in a water sample through the quartz plate (103) and generates protein fluorescence and/or humus fluorescence signals, the protein fluorescence signals are transmitted to the photodiode A (502) through the band-pass filter A (501), and the humus fluorescence signals are transmitted to the photodiode B (602) through the band-pass filter B (601);
(4) When the LED light source (7) outputs blue light, the blue light irradiates particles in a water sample through the quartz plate (103) to form scattered light signals, and the scattered light signals are transmitted into the photodiode B (602) through the band-pass filter B (601);
(5) Photodiode a (502) and/or photodiode B (602) convert the optical signal to an electrical signal output;
(6) The photoelectric signal operational amplifier module (803) processes the electric signals output by the photoelectric diode A (502) and/or the photoelectric diode B (602), amplifies the electric signals and transmits the amplified electric signals to the band-pass filter module (804); the band-pass filtering module (804) inputs the electric signal to the phase-sensitive detection module (805), then transmits the electric signal to the low-pass filtering module (806), and then converts the electric signal into a digital signal through the AD analog-to-digital conversion module (807);
(7) The singlechip (801) is used for collecting the digital signals and communicating with the upper computer to obtain on-line monitoring data of protein fluorescence, humus fluorescence and scattered light intensity signals, and calculating the ratio of the protein fluorescence signals to the humus fluorescence signals.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910035691.1A CN109540842B (en) | 2019-01-15 | 2019-01-15 | Double-fluorescence signal and water quality monitoring probe based on LED light source and use method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910035691.1A CN109540842B (en) | 2019-01-15 | 2019-01-15 | Double-fluorescence signal and water quality monitoring probe based on LED light source and use method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN109540842A CN109540842A (en) | 2019-03-29 |
| CN109540842B true CN109540842B (en) | 2023-09-19 |
Family
ID=65835268
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910035691.1A Active CN109540842B (en) | 2019-01-15 | 2019-01-15 | Double-fluorescence signal and water quality monitoring probe based on LED light source and use method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109540842B (en) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110542667B (en) * | 2019-09-29 | 2024-07-05 | 南京同开环保科技有限公司 | Portable rapid water quality detector and water quality detection method |
| CN114279979A (en) * | 2021-12-28 | 2022-04-05 | 长沙巨翊医疗科技有限公司 | Photoelectric probe and bilirubin concentration detector |
Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2430461A2 (en) * | 2009-05-15 | 2012-03-21 | Biomerieux, Inc | Combined detection instrument for culture specimen containers and instrument for identification and/or characterization of a microbial agent in a sample |
| CN102539091A (en) * | 2011-12-21 | 2012-07-04 | 合肥工业大学 | Filter bag breakage detector for bag type dust remover |
| CN104198391A (en) * | 2014-09-26 | 2014-12-10 | 南京大学 | Ultraviolet fluorescence double-signal water quality monitoring device taking LED (light emitting diode) as light source and application method of device |
| CN104730054A (en) * | 2015-04-10 | 2015-06-24 | 中国科学院烟台海岸带研究所 | Integrated probe type photoelectric water-quality multi-parameter online measuring system |
| CN104865231A (en) * | 2015-05-25 | 2015-08-26 | 南京信息工程大学 | Multichannel optical dissolved oxygen measurement device and measurement method |
| CN106092895A (en) * | 2016-08-23 | 2016-11-09 | 中国电子科技集团公司第四十九研究所 | A kind of water body chlorophyll concentration in situ detection device and detection method thereof |
| CN109085149A (en) * | 2018-10-24 | 2018-12-25 | 南京大学 | A kind of spectroscopic methodology water quality monitoring module and its application method based on LED light source |
| CN209400419U (en) * | 2019-01-15 | 2019-09-17 | 南京大学 | A kind of double fluorescence signals based on LED light source and Turbidity Water matter monitoring probe |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| IL155154A0 (en) * | 2000-09-30 | 2003-10-31 | Diversa Corp | Whole cell engineering by mutagenizing a substantial portion of a starting genome, combining mutations, and optionally repeating |
| US7319522B2 (en) * | 2004-05-27 | 2008-01-15 | Finesse Solutions Llc. | Systems and methods for in situ spectroscopic measurements |
-
2019
- 2019-01-15 CN CN201910035691.1A patent/CN109540842B/en active Active
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2430461A2 (en) * | 2009-05-15 | 2012-03-21 | Biomerieux, Inc | Combined detection instrument for culture specimen containers and instrument for identification and/or characterization of a microbial agent in a sample |
| CN102539091A (en) * | 2011-12-21 | 2012-07-04 | 合肥工业大学 | Filter bag breakage detector for bag type dust remover |
| CN104198391A (en) * | 2014-09-26 | 2014-12-10 | 南京大学 | Ultraviolet fluorescence double-signal water quality monitoring device taking LED (light emitting diode) as light source and application method of device |
| CN104730054A (en) * | 2015-04-10 | 2015-06-24 | 中国科学院烟台海岸带研究所 | Integrated probe type photoelectric water-quality multi-parameter online measuring system |
| CN104865231A (en) * | 2015-05-25 | 2015-08-26 | 南京信息工程大学 | Multichannel optical dissolved oxygen measurement device and measurement method |
| CN106092895A (en) * | 2016-08-23 | 2016-11-09 | 中国电子科技集团公司第四十九研究所 | A kind of water body chlorophyll concentration in situ detection device and detection method thereof |
| CN109085149A (en) * | 2018-10-24 | 2018-12-25 | 南京大学 | A kind of spectroscopic methodology water quality monitoring module and its application method based on LED light source |
| CN209400419U (en) * | 2019-01-15 | 2019-09-17 | 南京大学 | A kind of double fluorescence signals based on LED light source and Turbidity Water matter monitoring probe |
Non-Patent Citations (6)
| Title |
|---|
| Experimental study on the dynamic behavior of sandstone with coplanar elliptical flaws from macro, meso, and micro viewpoints;Han ZY et al;《RSC ADVANCES》;第120卷;1-12 * |
| Real-time self-tracking of an anticancer small molecule nanodrug based on colorful fluorescence variations;Wang ST et al;《RSC ADVANCES》;第6卷(第15期);12472-12478 * |
| Ultraviolet C upconversion fluorescence of trivalent erbium in BaGd2ZnO5 phosphor excited by a visible commercial light-emitting diode;Yang YM et al;《OPTICS LETTERS》;第39卷(第7期);2000-2003 * |
| 一种嵌入式净水系统在线水质监测装置的研发;张光延等;《电子器件》;第43卷(第2期);427-431 * |
| 深紫外水中溶解性有机物检测技术及其装置研究;张光延;《中国优秀硕士学位论文全文数据库 (工程科技Ⅱ辑)》(第2期);C038-2004 * |
| 红色荧光体增强型硅基光电二极管的频谱响应研究;李上宾等;《新能源进展》;第4卷(第5期);341-344 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN109540842A (en) | 2019-03-29 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US11092584B2 (en) | Composite LED module and water quality monitoring device using the same | |
| CN104198391B (en) | Ultraviolet fluorescence double-signal water quality monitoring device taking LED (light emitting diode) as light source and application method of device | |
| WO2020082628A1 (en) | Led light source-based spectrometric water quality monitoring module and use method thereof | |
| CN100541171C (en) | The method of ultraviolet cooperating with ozone measuring water body total nitrogen and total phosphorous by digestion spectrophotometry | |
| CN103323400A (en) | Multi-parameter integrated water quality on-line monitoring sensing system | |
| KR101748367B1 (en) | System for monitering Water Quality | |
| CN104880429A (en) | Online simultaneous monitoring sensor and online simultaneous monitoring method for COD (Chemical Oxygen Demand) and nitrogen content of nitrate | |
| CN105004701A (en) | Intelligent water quality monitor combining ultraviolet method and fluorescence method and usage method thereof | |
| CN106053421B (en) | Content of organic matter on-line checking and filter core/film breakdown early warning method and apparatus in water | |
| CN109540842B (en) | Double-fluorescence signal and water quality monitoring probe based on LED light source and use method | |
| US20090046287A1 (en) | Zero angle photo spectrophotometer for monitoring of water systems | |
| CN111678899A (en) | Fluorescence method dissolved oxygen sensor | |
| CN215066128U (en) | Drinking water quality detection device | |
| CN108507955A (en) | The device and method of multispectral synchronous detection chemical oxygen demand of water body | |
| CN209014471U (en) | A kind of spectroscopic methodology water quality monitoring module based on LED light source | |
| CN209387061U (en) | Composite package LED light source and water monitoring device based on the light source | |
| US20170205346A1 (en) | A fluorescent substance detection system | |
| CN209400419U (en) | A kind of double fluorescence signals based on LED light source and Turbidity Water matter monitoring probe | |
| CN112285047A (en) | Chemical oxygen demand and nitrate nitrogen water quality monitoring probe and method | |
| CN210720145U (en) | Portable quick water quality testing appearance | |
| JP2006300858A (en) | Water examination device | |
| Kang et al. | An ultra-high sensitivity fluorescence sensor based on fluorescence technology | |
| CN212780522U (en) | Portable water quality analyzer for soluble organic matters and turbidity | |
| CN213302014U (en) | Fluorescence method dissolved oxygen sensor | |
| CN112147101A (en) | Portable water quality analyzer and method for soluble organic matters and nitrate nitrogen |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |