CN116399861B - Device and method applied to synchronous detection of heavy metal ions - Google Patents
Device and method applied to synchronous detection of heavy metal ions Download PDFInfo
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- CN116399861B CN116399861B CN202310664394.XA CN202310664394A CN116399861B CN 116399861 B CN116399861 B CN 116399861B CN 202310664394 A CN202310664394 A CN 202310664394A CN 116399861 B CN116399861 B CN 116399861B
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- 238000001514 detection method Methods 0.000 title claims abstract description 105
- 229910001385 heavy metal Inorganic materials 0.000 title claims abstract description 47
- 150000002500 ions Chemical class 0.000 title claims abstract description 37
- 238000000034 method Methods 0.000 title claims abstract description 25
- 230000001360 synchronised effect Effects 0.000 title abstract description 7
- 239000007788 liquid Substances 0.000 claims abstract description 53
- 230000003287 optical effect Effects 0.000 claims abstract description 35
- 238000012545 processing Methods 0.000 claims abstract description 9
- 238000004891 communication Methods 0.000 claims abstract description 7
- 239000003153 chemical reaction reagent Substances 0.000 claims description 25
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 14
- 108091023037 Aptamer Proteins 0.000 claims description 13
- 239000000243 solution Substances 0.000 claims description 13
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 claims description 10
- 229910052737 gold Inorganic materials 0.000 claims description 10
- 239000010931 gold Substances 0.000 claims description 10
- 239000000758 substrate Substances 0.000 claims description 8
- 239000011780 sodium chloride Substances 0.000 claims description 7
- 238000002835 absorbance Methods 0.000 claims description 6
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 6
- 239000011259 mixed solution Substances 0.000 claims description 5
- 230000008569 process Effects 0.000 claims description 5
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- 238000011534 incubation Methods 0.000 claims description 4
- WFKWXMTUELFFGS-UHFFFAOYSA-N tungsten Chemical compound [W] WFKWXMTUELFFGS-UHFFFAOYSA-N 0.000 claims description 4
- 229910052721 tungsten Inorganic materials 0.000 claims description 4
- 239000010937 tungsten Substances 0.000 claims description 4
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Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
- G01N21/78—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a change of colour
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/50273—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the means or forces applied to move the fluids
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
- G01N21/31—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2400/00—Moving or stopping fluids
- B01L2400/04—Moving fluids with specific forces or mechanical means
- B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
- B01L2400/0409—Moving fluids with specific forces or mechanical means specific forces centrifugal forces
Abstract
The application provides a device and a method applied to synchronous detection of heavy metal ions, comprising the following steps: the microfluidic chip comprises a structural layer, at least N detection units are arranged in an annular array mode relative to the rotation center of the structural layer, the detection units comprise a first liquid storage chamber and a second liquid storage chamber, the first liquid storage chamber and the second liquid storage chamber are respectively communicated with the detection chambers, a low-rotation-speed capillary valve and a high-rotation-speed capillary valve are arranged on communication paths of the first liquid storage chamber, the second liquid storage chamber and the detection chambers, and N is a positive integer; the centrifugal rotation module is used for driving the microfluidic chip to perform rotary motion; the optical detection module comprises a light source, an emission light collimating lens arranged above the micro-fluidic chip and a receiving light collimating lens arranged below the micro-fluidic chip, wherein the light of the light source passes through the emission light collimating lens and the detection chamber and irradiates the receiving light collimating lens; and the control and processing module is used for controlling the centrifugal rotation module and receiving the micro spectrometer signals.
Description
Technical Field
The application relates to the field of heavy metal detection, in particular to a device and a method for synchronously detecting heavy metal ions.
Background
With the acceleration of industrialization progress, water quality safety is receiving more and more attention. Among them, heavy metal pollution has become a serious threat to water quality safety. Heavy metal pollutants in water are difficult to degrade in the environment, can be accumulated in animals and plants, are gradually enriched through a food chain, have the concentration of thousands of or even millions of times and finally enter the human body to cause harm.
Traditional heavy metal detection methods comprise an Atomic Absorption Spectrometry (AAS), an inductively coupled plasma mass spectrometry (ICP-MS), an inductively coupled plasma atomic emission spectrometry (ICP-AES), an Atomic Fluorescence Spectrometry (AFS) and the like, and the methods have high sensitivity and good accuracy, but the methods need to rely on large detection equipment and professional operators for operation, have higher detection and maintenance cost and longer detection period.
The optical sensor has become a good platform for detecting the concentration of heavy metals due to small volume, chemical inertness and strong electromagnetic interference resistance. Colorimetric methods based on gold nanometers (AU NPS) are widely developed and utilized due to their direct color indication and high sensitivity.
The conventional gold nano (AU NPS) colorimetric method detection system requires complicated reagent mixing, reaction and incubation processes, requires professional personnel to operate, and requires a special detection environment, so that the portable rapid detection of heavy metal ions is difficult to realize.
Disclosure of Invention
The application provides a device and a method for synchronously detecting heavy metal ions, which aim to solve the problem that a professional is required to mix reagents when a gold nano detection method is adopted and reduce the difficulties of mixing, reacting and incubating the reagents.
In order to achieve the above object, an embodiment of the present application provides a device for synchronous detection of heavy metal ions, including:
the microfluidic chip comprises a substrate layer, a structural layer and a cover plate layer which are stacked from bottom to top, wherein at least N detection units are arranged on the structural layer, the N detection units are distributed in an annular array with respect to the rotation center of the structural layer, each detection unit comprises a first liquid storage chamber and a second liquid storage chamber, the first liquid storage chamber and the second liquid storage chamber are respectively communicated with the detection chambers, a low-rotation-speed capillary valve and a high-rotation-speed capillary valve are respectively arranged on communication paths of the first liquid storage chamber, the second liquid storage chamber and the detection chambers, and N is a positive integer less than or equal to 6;
the centrifugal rotation module is used for connecting the rotation center of the microfluidic chip and driving the microfluidic chip to perform rotary motion;
the optical detection module comprises a light source, an emission light collimating lens arranged above the micro-fluidic chip and a receiving light collimating lens arranged below the micro-fluidic chip, wherein the emission light collimating lens and the receiving light collimating lens are collinear in the vertical direction, and the light of the light source passes through the emission light collimating lens, the detection chamber and irradiates on the receiving light collimating lens;
the control and processing module comprises a PLC and a micro spectrometer, wherein the PLC is respectively connected with the centrifugal rotating module and the micro spectrometer in a signal mode, and the micro spectrometer acquires the spectrum of the received light collimating lens.
Preferably, the substrate layer, the structural layer and the cover plate layer are all made of transparent plates, the cover plate layer is provided with a first air inlet hole formed above the first liquid storage chamber and the second liquid storage chamber, and the cover plate layer is provided with a second air inlet hole and a second air outlet hole formed above the detection chamber.
Preferably, the centrifugal rotating module comprises a servo motor, a motor support and a chip clamp, wherein the servo motor is arranged on the motor support, the chip clamp is arranged at the output end of the servo motor and is used for clamping the microfluidic chip, and the servo motor is connected with the PLC through signals.
The application also provides a method for synchronously detecting the heavy metal ions, which adopts the device for synchronously detecting the heavy metal ions and comprises the following steps:
s1, respectively adding an aptamer reagent, a sodium chloride reagent and a gold nano reagent into a first liquid storage chamber, a second liquid storage chamber and a detection chamber;
s2, dripping a solution to be detected into the first liquid storage chamber, and clamping the microfluidic chip on the centrifugal rotation module;
s3, the centrifugal rotating module rotates at a first rotating speed R1 until the solution to be detected and the aptamer reagent are uniformly mixed with each other in a first state of uniform circular motion; the centrifugal rotating module rotates at a second rotating speed R2, the low rotating speed capillary valve is opened, and the mixed solution in the first liquid storage chamber enters the detection chamber and is mixed in the detection chamber; the centrifugal rotating module rotates at a third rotating speed R3, a high rotating speed capillary valve is opened, and sodium chloride reagent enters a detection chamber to be mixed;
s4, the optical detection module acquires optical data in the detection chamber, brings the optical data into a linear fitting curve of the absorbance value of the detection system corresponding to the concentration of the heavy metal ions, and finally converts the linear fitting curve into a digital signal corresponding to the concentration of the heavy metal ions of the detection unit and displays the digital signal.
Preferably, before the step S2 starts, 10ML, 20ML and 50ML of deionized water are dropped into the first liquid storage chamber, the second liquid storage chamber and the detection chamber, respectively.
Preferably, the relationship among the first rotation speed, the second rotation speed and the third rotation speed is R1 < R2 < R3.
Preferably, the first rotation speed R1 is 300RMP, the rotation time is 60S, and the microfluidic chip is stationary for 15min after rotation to complete the incubation process of the aptamer and the heavy metal ions; the second rotating speed R2 is 500RMP, the rotating time is 60S, and the micro-fluidic chip is static for 10min after rotating to complete the reaction process of the aptamer and the gold nanometer; the third rotational speed R3 is 800RMP and the rotational time is 60S.
Preferably, the centrifugal rotating module is initialized and rotated in a second state, the second state is intermittent fixed-angle rotation, the fixed-angle rotation angle is 360 °/N, the time between adjacent fixed-angle rotations is 2S, during the period that the centrifugal rotating module is stopped, the light source vertically irradiates the detection chamber through the emission light collimating lens, the receiving light collimating lens vertically receives the transmitted light, and the detected optical signal is transmitted to the spectrum driver to obtain optical data, and the PLC converts the obtained optical data into a digital signal corresponding to the concentration of the heavy metal ions of the detection unit by processing the obtained optical data and bringing the optical data into a linear fitting curve corresponding to the absorbance value of the detection system.
Preferably, the light source is a halogen tungsten lamp with the wavelength of 380-2500nm.
The scheme of the application has the following beneficial effects:
according to the application, different capillary valves are opened by using the detection unit at different rotating speeds, so that various reagents can sequentially enter the detection chamber, an experimenter can realize the mixing of a solution to be detected and the reagents by only adjusting the rotating speed of the centrifugal rotating module, the difficulty of reagent mixing is reduced, and the heavy metal detection can be realized conveniently and rapidly.
Additional features and advantages of the application will be set forth in the detailed description which follows.
Drawings
FIG. 1 is an external schematic view of a device for synchronous detection of heavy metal ions;
FIG. 2 is a schematic diagram of the interior of the device for synchronous detection of heavy metal ions;
FIG. 3 is a schematic diagram of a microfluidic chip and a centrifugal spin module;
FIG. 4A is a schematic diagram of a microfluidic chip structure;
FIG. 4B is a schematic diagram of a structural layer;
fig. 5 is a schematic diagram of a chip holder.
[ reference numerals description ]
Wherein, 1 front baffle; 2, a bottom plate; 3, a left side plate; 4, a rear baffle; 5, a baffle plate is arranged; 6, a microfluidic chip; 61 cover sheet layers; 62 structural layers; 63 substrate layers; 621 a first reservoir; 622 low rotational speed capillary valve; 623 a second reservoir; 624 high rotational speed capillary valve; 625 detection chamber; 8, a chip clamp; 81 clamping caps on the clamps; 82 clamp positioning blocks; 83 clamp motor connecting cylinder; 9, touching a display screen baffle; 10 touch display screen; 11 a device power supply main switch; 12 micro spectrometer power switch; 13 a light source power switch; 14 corner fixing members; 17 light sources; 18 miniature spectrometer support; 19 micro spectrometer; 21 raspberry group development board; 22 power charging interfaces; a 23 power supply; a 24 power supply bracket; 25 servo motors; 26 motor support; 27 receiving a light collimating lens; 28 an emission light collimating lens;
Detailed Description
In order to make the technical problems, technical solutions and advantages to be solved more apparent, the following detailed description will be given with reference to the accompanying drawings and specific embodiments.
As shown in fig. 1-5, an embodiment of the present application provides a device for synchronous detection of heavy metal ions, which includes a microfluidic chip 6, a centrifugal rotation module, an optical detection module, and a control and processing module, where the microfluidic chip 6 includes a substrate layer 63 glued from bottom to top, a structure layer 62, and a cover layer 61, where N detection units are disposed on the structure layer 62, and the N detection units are arranged in an annular array about a rotation axis of the structure layer 62. Each detection unit comprises a first liquid storage chamber 621, a second liquid storage chamber 623 and a detection chamber 625, wherein the first liquid storage chamber 621 is communicated with the detection chamber 625 through a first communication pipeline, the second liquid storage chamber 623 is communicated with the detection chamber 625 through a second communication pipeline, a low-rotation-speed capillary valve 622 is arranged on the first communication pipeline, a high-rotation-speed capillary valve 624 is arranged on the second communication pipeline, and the opening threshold value of the low-rotation-speed capillary valve 622 is smaller than that of the high-rotation-speed capillary valve 624. N is a positive integer and N is less than or equal to 6, in this embodiment n=6.
In this embodiment, the detection chamber 625 is further from the center of rotation of the structural layer 62 than the first liquid storage chamber 621 or the second liquid storage chamber 623.
The centrifugal rotation module is used for connecting the rotation center of the microfluidic chip 6 and driving the microfluidic chip 6 to perform rotary motion.
The optical detection module comprises a light source 17, an emission light collimating lens 28 and a receiving light collimating lens 27, wherein the emission light collimating lens 28 and the receiving light collimating lens 27 are respectively arranged above and below the microfluidic chip 6, and the emission light collimating lens 28 and the receiving light collimating lens 27 are collinear in the vertical direction, when the light source 17 emits light, the light passes through the detection chamber 625 through refraction of the emission light collimating lens 28, and the receiving light collimating lens 27 receives the light below the detection chamber 625.
The control and processing module comprises a PLC and a micro spectrometer 19, the PLC is connected with the centrifugal rotation module and the micro spectrometer 19 through signals, and the micro spectrometer 19 acquires the spectrum of the receiving light collimating lens 27.
In the application, different reagents can be sequentially dripped into the detection chamber 625 by utilizing the microfluidic chip 6 at different rotating speeds, so that the sequential dripping of the reagents is realized, and the heavy metal detection can be realized without a professional technology.
Specifically, the substrate layer 63, the structure layer 62 and the cover plate layer 61 are colorless and transparent PMMA plates, wherein the substrate layer 63 is made of PMMA plates with a thickness of 0.55MM and a diameter of 78MM, the lowermost layer of the microfluidic chip 6 is located, the structure layer 62 is made of PMMA plates with a thickness of 2MM and a diameter of 78MM, 6 detection units are disposed on the structure layer 62, and each detection unit is annularly arranged with respect to the rotation center of the structure layer 62. The cover plate layer 61 is made of PMMA plate with thickness of 0.5MM and diameter of 78MM and is positioned at the uppermost layer of the microfluidic chip 6. The cover plate layer 61 is formed with first air inlet holes above the first and second liquid storage chambers 621 and 623, and the cover plate layer 61 is formed with second air inlet holes and second air outlet holes above the detection chamber 625.
The application further comprises a box body, wherein the box body comprises a front baffle plate 1, a bottom plate 2, a left side plate 3, a right side plate, a rear baffle plate 4, an upper baffle plate 5 and a touch display screen baffle plate 9, all the plate surfaces are connected through corner fixing pieces 14 and surround to form the box body, a touch display screen 10 is arranged on the touch display screen baffle plate 9, and the touch display screen 10 is in signal connection with a PLC.
Set up centrifugal rotatory module, optical detection module and control and processing module in the box, centrifugal rotatory module includes servo motor 25, chip anchor clamps 8 and motor support 26, wherein motor support 26 sets up on bottom plate 2, servo motor 25 installs on motor support 26, chip anchor clamps 8 include clamp on the anchor clamps tight cap 81, anchor clamps locating piece 82 and anchor clamps motor connecting cylinder 83, anchor clamps locating piece 82 sets up in the top of anchor clamps motor connecting cylinder 83, when microfluidic chip 6 installs on anchor clamps locating piece 82, clamp on the anchor clamps tight cap 81 with microfluidic chip 6 crimping on anchor clamps locating piece 82. The clamp motor connecting cylinder 83 is mounted on the output shaft of the servo motor 25, and transmits the power of the servo motor 25 to the micro-fluidic chip 6 to drive the micro-fluidic chip 6 to perform rotary motion.
The optical detection module includes a light source power switch 13, and the light source power switch 13 is electrically connected to the light source 17 to control on/off of the light source 17. The light source 17 is a halogen tungsten lamp, and can emit initial light with a wavelength range of 380-2500nm, and the initial light passes through each detection chamber 625 and then is collected and recorded by a micro spectrometer 19 through which light intensity signals with a wavelength range of 350-800nm are transmitted.
Preferably, the micro-spectrometer 19 is mounted by a micro-spectrometer mount 18.
The control and processing module comprises a PLC, in this embodiment, the PLC adopts a raspberry group development board 21, a servo motor driving unit is arranged on the raspberry group development board 21 to control the working state of a servo motor 25, and a spectrometer driving unit is used for controlling the working state of a micro spectrometer 19. The PLC further comprises a receiving unit for receiving the spectral information obtained by the micro spectrometer 19 and an arithmetic unit for converting the spectral information into readable data.
The application also comprises a power supply module which comprises a power supply 23, a power supply bracket 24, a device power supply main switch 11, a micro spectrometer power supply switch 12, a light source power supply switch 13 and a power supply charging port interface 22. The power module is used for providing power for each module and can be assembled and connected according to the prior art.
The application also provides a method for synchronously detecting the heavy metal ions, which adopts the device for synchronously detecting the heavy metal ions and comprises the following steps:
step one: aptamer reagent, sodium chloride reagent and gold nano-reagent are added to the first liquid storage chamber 621, the second liquid storage chamber 623 and the detection chamber 625, respectively, and freeze-drying treatment is performed.
Before dropping the solution to be measured into the first liquid storage chamber 621, 10ML, 20ML and 50ML of deionized water are added to the first liquid storage chamber 621, the second liquid storage chamber 623 and the detection chamber 625, respectively, for dissolving the lyophilized reagent.
And step two, dripping the solution to be tested into the first liquid storage chamber 621, and clamping the microfluidic chip 6 on the centrifugal rotation module.
And thirdly, the centrifugal rotating module works in a first state, and the first state is uniform circular motion. The centrifugal rotating module rotates at a first rotating speed R1 until the solution to be detected and the aptamer reagent are uniformly mixed; the centrifugal rotation module rotates at a second rotation speed R2 to reach the threshold value that the low-rotation-speed capillary valve 622 is opened, and the mixed solution in the first liquid storage chamber 621 enters the detection chamber 625 to be mixed; finally, the centrifugal rotating module rotates at the third rotating speed R3, the opening threshold of the high-rotating-speed capillary valve 624 is reached, and the sodium chloride reagent enters the detection chamber 625 to be mixed.
Specifically, the centrifugal rotation module rotates for 60S at the first rotation speed R1, so that the solution to be detected in the first liquid storage chamber 621 is uniformly mixed with the aptamer solution, and the incubation process of the aptamer and the heavy metal ions is completed in a static state for 15 minutes; and then rotating for 60S at the second rotation speed R2, the mixed solution in the first liquid storage chamber 621 breaks through the low rotation speed capillary valve 622 to realize that the mixed solution in the first liquid storage chamber 621 flows to the detection chamber 625 and is uniformly mixed in the detection chamber 625, and the reaction process of the aptamer and the gold nanometer is completed in a static state for 10 minutes. Under the condition that the rotating speed R3 rotates for 60S, the sodium chloride solution in the second liquid storage chamber 623 breaks through the high-rotating-speed capillary valve 624, so that the solution in the second liquid storage chamber 623 flows to the detection chamber 625 and induces gold nano aggregation.
The relationship among the first rotation speed, the second rotation speed and the third rotation speed is that R1 < R2 < R3, in this embodiment, R1 is 300RMP, R2 is 500RMP, and R3 is 800RMP.
And step four, the optical detection module acquires optical data in the detection chamber 625, the optical data is brought into a linear fitting curve of the absorbance value of the detection system corresponding to the concentration of the heavy metal ions, and finally the linear fitting curve is converted into a digital signal corresponding to the concentration of the heavy metal ions of the detection unit and displayed.
Specifically, the centrifugal rotation module is initialized and continues to rotate in a second state, the second state is intermittent fixed-angle rotation, the fixed-angle rotation angle is 360 degrees/N, and the time of the adjacent fixed-angle rotation interval is 2S.
In this embodiment, the centrifugal rotation module rotates in the second state, after the first rotation, the detection chamber 625 is located under the emission collimating lens 28 during the stay 2S, the light source 17 irradiates the detection chamber 625 vertically through the emission collimating lens 28, and the light source receives the transmitted light vertically by the receiving collimating lens 27, and transmits the detected optical signal to the micro spectrometer 19 to obtain optical data, and the PLC converts the obtained optical data into a digital signal corresponding to the concentration of heavy metal ions in the detection unit by processing the obtained optical data and bringing the obtained optical data into a linear fitting curve corresponding to the concentration of heavy metal ions in the absorbance value of the detection system, thereby achieving the final detection purpose.
In this embodiment, the light source 17 is a halogen tungsten lamp with a wavelength of 380-2500nm.
The heavy metal detection device and method provided by the application can be used for detecting heavy metals such as lead, mercury and cadmium, and the like, the micro-fluidic chip 6 is clamped on the centrifugal rotation module, the heavy metal detection reagents of different chambers in the micro-fluidic chip 6 are sequentially sampled, mixed and reacted under the action of centrifugal force by controlling the rotation speed and the rotation time regulation and control of the servo motor 25, the optical signals of the detection chambers 625 of each detection unit are collected through the optical signals, the collected optical signals are converted into the concentration information of heavy metal ions by the PLC, and finally the concentration of the heavy metal ions is output.
While the foregoing is directed to the preferred embodiments of the present application, it will be appreciated by those skilled in the art that various modifications and adaptations can be made without departing from the principles of the present application, and such modifications and adaptations are intended to be comprehended within the scope of the present application.
Claims (5)
1. The method for synchronously detecting the heavy metal ions is characterized by comprising a device applied to synchronously detecting the heavy metal ions, wherein the device applied to synchronously detecting the heavy metal ions comprises the following steps:
the micro-fluidic chip (6) comprises a substrate layer (63), a structural layer (62) and a cover plate layer (61) which are stacked from bottom to top, wherein at least N detection units are arranged on the structural layer (62), the N detection units are arranged in an annular array with respect to the rotation center of the structural layer (62), each detection unit comprises a first liquid storage chamber (621) and a second liquid storage chamber (623), the first liquid storage chambers (621) and the second liquid storage chambers (623) are respectively communicated with a detection chamber (625), and a low-rotation-speed capillary valve (622) and a high-rotation-speed capillary valve (624) are respectively arranged on the communication paths of the first liquid storage chambers (621), the second liquid storage chambers (623) and the detection chambers (625), and N is a positive integer less than or equal to 6;
the centrifugal rotation module is used for connecting the rotation center of the micro-fluidic chip (6) and driving the micro-fluidic chip (6) to perform rotary motion;
the optical detection module comprises a light source (17), an emission light collimating lens (28) arranged above the microfluidic chip (6) and a receiving light collimating lens (27) arranged below the microfluidic chip (6), wherein the emission light collimating lens (28) and the receiving light collimating lens (27) are collinear in the vertical direction, and the light of the light source (17) passes through the emission light collimating lens (28) and the detection chamber (625) and irradiates the receiving light collimating lens (27);
the control and processing module comprises a PLC and a micro spectrometer (19), wherein the PLC is respectively connected with the centrifugal rotation module and the micro spectrometer (19) in a signal mode, and the micro spectrometer (19) acquires the spectrum of the received light collimating lens (27);
the substrate layer (63), the structural layer (62) and the cover plate layer (61) are all made of transparent plates, the cover plate layer (61) is provided with a first air inlet hole above the first liquid storage chamber (621) and the second liquid storage chamber (623), and the cover plate layer (61) is provided with a second air inlet hole and a second air outlet hole above the detection chamber (625);
the centrifugal rotation module comprises a servo motor (25), a motor bracket (26) and a chip clamp (8), wherein the servo motor (25) is arranged on the motor bracket (26), the chip clamp (8) is arranged at the output end of the servo motor, the chip clamp (8) is used for clamping the micro-fluidic chip (6), and the servo motor (25) is in signal connection with the PLC;
the method comprises the following steps:
s1, respectively adding an aptamer reagent, a sodium chloride reagent and a gold nano reagent into a first liquid storage chamber (621), a second liquid storage chamber (623) and a detection chamber (625);
s2, dripping a solution to be detected into the first liquid storage chamber (621), and clamping the microfluidic chip (6) on the centrifugal rotation module;
s3, the centrifugal rotating module rotates at a first rotating speed R1 until the solution to be detected and the aptamer reagent are uniformly mixed with each other in a first state of uniform circular motion; the centrifugal rotation module rotates at a second rotation speed R2, the low-rotation-speed capillary valve (622) is opened, and the mixed solution in the first liquid storage chamber (621) enters the detection chamber (625) and is mixed in the detection chamber (625); the centrifugal rotating module rotates at a third rotating speed R3, a high rotating speed capillary valve (624) is opened, and sodium chloride reagent enters a detection chamber (625) to be mixed;
the relation among the first rotating speed, the second rotating speed and the third rotating speed is R1 < R2 < R3;
s4, the optical detection module acquires optical data in the detection chamber (625), brings the optical data into a linear fitting curve of the absorbance value of the detection system corresponding to the concentration of the heavy metal ions, and finally converts the linear fitting curve into a digital signal corresponding to the concentration of the heavy metal ions of the detection unit and displays the digital signal.
2. The method for synchronously detecting heavy metal ions according to claim 1, wherein the method comprises the following steps: before starting step S2, 10ML, 20ML and 50ML of deionized water are dropped into the first liquid storage chamber (621), the second liquid storage chamber (623) and the detection chamber (625), respectively.
3. The method for synchronously detecting heavy metal ions according to claim 2, wherein the method comprises the following steps: the first rotating speed R1 is 300RMP, the rotating time is 60S, and the microfluidic chip (6) is static for 15min after rotating to complete the incubation process of the aptamer and the heavy metal ions; the second rotating speed R2 is 500RMP, the rotating time is 60S, and the micro-fluidic chip (6) is static for 10min after rotating to complete the reaction process of the aptamer and the gold nanometer; the third rotational speed R3 is 800RMP and the rotational time is 60S.
4. The method for synchronously detecting heavy metal ions according to claim 3, wherein the method comprises the following steps: the centrifugal rotating module is initialized and rotated in a second state, the second state is intermittent fixed-angle rotation, the fixed-angle rotation angle is 360 degrees/N, the time between adjacent fixed-angle rotation intervals is 2S, during the period that the centrifugal rotating module stops, the light source (17) vertically irradiates the detection chamber (625) through the emission light collimating lens (28), the receiving light collimating lens (27) vertically receives transmitted light, and detected optical signals are transmitted to the spectrum driver to acquire optical data, and the PLC processes the acquired optical data and brings the acquired optical data into a linear fitting curve corresponding to the concentration of heavy metal ions in the absorbance value of the detection system and converts the optical data into digital signals corresponding to the concentration of the heavy metal ions in the detection unit.
5. The method for synchronously detecting heavy metal ions according to claim 4, wherein the method comprises the following steps: the light source (17) is a halogen tungsten lamp with the wavelength of 380-2500nm.
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